Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0008791

Physical Location & Seq

Genomic Coordinates Reverse (-)

27786602 .. 27791052

Gene Structure 4451 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1196800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2298 bp

ATGACTTGTATTGTTGGAGTTTCGTCTGTATATGCCCAGAGTCCGTGCAGCGAGCTGTACAGAAGAGCGGCGGCGCCGGCTGCGGCAACGACGAGCAGTGCTACTCTTTCGTTGGCGTTTCCCGAGAGGCCTCACTTCAACAGTGTTTTGAGAGGCAAGAGTGGGTTGGTCAGGCAGGGCTCCGGTGTCGTTTGTTCGGCCATTGCCACTCCGAACTCGGTGCTCAGTGAAGAGGCGTTTAAGGGATTCGGTGACTTTTCTAAGGACTCGCTTGACTCCGACTCGGAGTACGAGTCGGAAACTGAGTCGGGTTCCGCTGGTAGTGACGACGATGAGCTCGCTCTTTCCAAGCTGGGGTTGCCTCAGCGGCTTGTTGATAGCCTTGAGAGACGTGGCATTTCGAGCCTTTTCCCAATTCAGAGAGCTGTGCTAATACCTGCACTGGAAGGTCGAGATATCATTGCTCGTGCGAAGACGGGGACTGGAAAGACATTAGCCTTCGGAATCCCAATACTCAAGCGTCTCACAGAAGATGATGAACAGAGAGGCTCTCATAGGCGGACCGGTTATCTTCCTAGAGTTTTGGTGCTTGCACCTACACGGGAGTTGGCAAAGCAAGTTGAAAAGGAGATGAAAGAATCTGCACCTTACCTAAACACTGTTTGTGTCTATGGGGGAGTTTCCTACATAACACAACAAAGTGCTCTCTCCCGTGGAGTTGATGTAGTTGTTGGAACTCCTGGTCGGATTATTGACCTGATAAACGGGAACAGTCTCAAATTGGGCGAAGTTCAATATTTGGTACTTGATGAAGCTGATTCGATGCTTGCTGTTGGATTTGAGGAGGATGTGGAAGTGATTCTACAAAAGCTTCCAGCTCAGAGGCAGAGCATGCTTTTTTCTGCAACCATGCCTGGTTGGGTGAAAAAATTATCACGGAAATATTTGGACAATCCATTGACAATTGACTTGGTTGGCGATCAAGACGAGAAGCTTGCAGAAGGGATCAAACTTTATGCTTTATCAACCACTTCAGCTTCAAAGAAGACCATTCTTAGTGATCTTATAACAGTGTATGCAAAGGGTGGGAAGACCATTGTATTTACACAAACGAAACGAGATGCTGATGCAGTCTCAATGTCATTAACAAGTAGCATAGCTTCTGAGGCATTGCATGGAGATATATCACAGAATCAAAGAGAGAGAACATTAAATGGTTTTCGGCAAGGGAAATTCACTGTGCTTGTTGCCACTGATGTTGCATCCCGGGGACTTGATATTCCCAATGTCGATTTGGTTATCCACTACGAGCTTCCCAATGATTCAGAGACATTTGTGCATCGCTCTGGGCGTACTGGACGTGCAGGGAAACAAGGTACTGCCGTTCTGATGTTTACGAACAACCAGAGGAGAACAATTAAAACTCTTGAGCGTGATGTGGGGTGCAAGTTTGAGTTTGCTAGTCCACCAACTATGGAGGAGGTTTTGGAGTCATCTGCTCAGCACGTTGTTGCTACTCTTAGCGGAGTGCATCCTGAATCTGTACAGTTTTTCACACCAACGGCACAGAGATTGATTGATGAACAGGGAACAAGTGCTCTTGCTGCTGCACTTGCACAGCTCAGTGGATTTTCTCGTCCTCCATCATCCAAGTCCCTTATCACTCATGAGGCGGGATGGACGACATTGCAACTTATCCGAGATCCATCGTTTGCCAGAGGGTTCCTGTCTGCAAGATCTGTCACTGGGTTTCTTTCCGATGTTTATTCTACAGCTGCTGATGAAGTTGGAAAAATACATATAATTGCAGATGAACGGATTCAAGGAGCAGTTTTTGATCTTCCAGAGGAGATTGCCAAAGAGTTGCTGACCAGGCAAATACCCCCTGGAAATACTATTTCCAAGATATCCAAGTTGCCAGCCCTGCAAGATGATGGGCCGGTGAGTGACAACTATGGCAGGTTTTCAGGCAGAGATCGCCGGGGAGGAGGCCGTGGAGGAGGCCGGGGTGGAGGTTTTAGGGATCGTCAAGGATCATCAGGTGGTTTTAGAAGCTCCAGGGGTTGGGGTAGTGATGGTGGTGCCGATGATTCATTTAGGAGTAGTGGCCGAAGTTATGGATCTAGCAACAGCAGACCTCGGAGTCCAAGAAGCATCGATGACGATTGGCTTATTGGAGGCCAACGATCAAGCAGGTCACCATCCCGAGACAGTTCCAGAAGCTTTGGAGGTTCCTGCTTCAATTGTGGGCGCTCCGGCCACAGGGCATCAGATTGCCCTACCAAACAAGGATTCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000373 GO:0000375 GO:0000377 GO:0003674 GO:0003676 GO:0003723 GO:0003724 GO:0003729 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0006970 GO:0006996 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0008380 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009628 GO:0009651 GO:0009657 GO:0009658 GO:0009941 GO:0009987 GO:0010467 GO:0010501 GO:0016020 GO:0016043 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0022613 GO:0031967 GO:0031974 GO:0031975 GO:0031981 GO:0034641 GO:0042254 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0050896 GO:0070013 GO:0070035 GO:0071704 GO:0071840 GO:0090304 GO:0097159 GO:0140098 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

ko01110 map01110

Pfam Domains

Protein Families

DEAD GUCT Helicase_C zf-CCHC

Protein Analysis

766

Amino Acids

82.31

Weight (kDa)

8.27

Isoelectric Point (pI)

46.04

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	138 - 310	9.5e-45	DEAD/DEAH box helicase
ResIII	PF04851	151 - 304	1.2e-06	Type III restriction enzyme, res subunit
Helicase_C	PF00271	348 - 456	6e-32	Helicase conserved C-terminal domain
DD_DDX21-DDX50	PF26142	492 - 540	9.7e-16	DDX21/DDX50 dimerisation domain
GUCT	PF08152	543 - 643	8.2e-15	GUCT (NUC152) domain
zf-CCHC	PF00098	745 - 760	2.7e-06	Zinc knuckle

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008791)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G26742 AT5G26742 AT5G26742
fragaria_vesca	FvH4_6g08810 FvH4_6g08810 FvH4_6g08810 FvH4_6g08810
malus_domestica	MD04G1183400.v1.1 MD12G1196800.v1.1
prunus_persica	Prupe.6G301900_v2.0.a1
pyrus_communis	pycom04g16190 pycom12g18300
rosa_chinensis	RchiOBHm_Chr3g0459081
rosa_laevigata	RLG00000025121
rosa_multiflora	Rmu_sc0000466.1_g000002 Rmu_sc0003515.1_g000001
rosa_roxburghii	Rroxscaffold_6G00420530
rosa_rugosa	Rorug03G0033400
rosa_samantha	Rh3AG091300 Rh3BG094400 Rh3CG094800 Rh3DG094800
rosa_wichuraiana	Rw3G007840

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1067
AasI	GACNNNNNNGTC	1 cut(s)	2142
Acc36I	ACCTGC	3 cut(s)	445, 1950, 2184
AccB1I	GGYRCC	2 cut(s)	73, 2081
AccB7I	CCANNNNNTGG	1 cut(s)	2064
AccBSI	CCGCTC	1 cut(s)	68
AciI	CCGC	8 cut(s)	68, 71, 83, 315, 367, 559, 1524, 1673
AclWI	GGATC	5 cut(s)	1013, 1697, 2031, 2041, 2128
AcoI	YGGCCR	3 cut(s)	198, 2107, 2257
AcsI	RAATTY	1 cut(s)	1232
AcuI	CTGAAG	1 cut(s)	1017
AcyI	GRCGYC	1 cut(s)	74
AfaI	GTAC	6 cut(s)	59, 290, 804, 1354, 1378, 1545
AfiI	CCNNNNNNNGG	3 cut(s)	354, 2064, 2262
AgeI	ACCGGT	1 cut(s)	563
AgsI	TTSAA	6 cut(s)	139, 623, 794, 1041, 1823, 2242
AjiI	CACGTC	2 cut(s)	392, 1361
AjnI	CCWGG	5 cut(s)	739, 913, 1871, 1885, 2057
AjuI	GAANNNNNNNTTGG	1 cut(s)	2276
Alw21I	GWGCWC	4 cut(s)	225, 339, 706, 1600
Alw26I	GTCTC	6 cut(s)	382, 527, 779, 1138, 1322, 2202
AlwI	GGATC	5 cut(s)	1013, 1697, 2031, 2041, 2128
AlwNI	CAGNNNCTG	1 cut(s)	1778
Ama87I	CYCGRG	3 cut(s)	122, 1266, 2205
AoxI	GGCC	8 cut(s)	128, 198, 1937, 1990, 2002, 2107, 2179, 2257
ApeKI	GCWGC	5 cut(s)	48, 80, 1604, 1607, 1775
ApoI	RAATTY	1 cut(s)	1232
AsiGI	ACCGGT	1 cut(s)	563
Asp700I	GAANNNNTTC	2 cut(s)	858, 1818
AspLEI	GCGC	2 cut(s)	76, 2253
AspS9I	GGNCC	2 cut(s)	561, 1937
AsuC2I	CCSGG	4 cut(s)	1267, 1268, 1982, 2006
AsuHPI	GGTGA	4 cut(s)	263, 934, 1954, 2190
AvaI	CYCGRG	3 cut(s)	122, 1266, 2205
AvaII	GGWCC	1 cut(s)	561
BaeI	ACNNNNGTAYC	2 cut(s)	1368, 1401
BanI	GGYRCC	2 cut(s)	73, 2081
BanII	GRGCYC	2 cut(s)	182, 339
BarI	GAAGNNNNNNTAC	4 cut(s)	846, 878, 1144, 1176
BauI	CACGAG	1 cut(s)	465
BbsI	GAAGAC	3 cut(s)	479, 1052, 1097
Bbv12I	GWGCWC	4 cut(s)	225, 339, 706, 1600
BbvCI	CCTCAGC	1 cut(s)	363
BbvI	GCAGC	5 cut(s)	60, 67, 1591, 1594, 1762
BccI	CCATC	6 cut(s)	1651, 1671, 1714, 1928, 2069, 2209
BceAI	ACGGC	3 cut(s)	1367, 1578, 1977
BcgI	CGANNNNNNTGC	4 cut(s)	202, 236, 977, 1011
BciT130I	CCWGG	5 cut(s)	741, 915, 1873, 1887, 2059
BcnI	CCSGG	4 cut(s)	1267, 1268, 1982, 2006
BcoDI	GTCTC	6 cut(s)	382, 527, 779, 1138, 1322, 2202
BfaI	CTAG	4 cut(s)	576, 1461, 2124, 2296
BfmI	CTRYAG	1 cut(s)	1770
BfoI	RGCGCY	2 cut(s)	77, 2254
BfuAI	ACCTGC	3 cut(s)	445, 1950, 2184
BglI	GCCNNNNNGGC	1 cut(s)	367
BglII	AGATCT	1 cut(s)	1736
BisI	GCNGC	9 cut(s)	49, 69, 72, 81, 84, 368, 1605, 1608, 1776
BlpI	GCTNAGC	1 cut(s)	1500
BlsI	GCNGC	9 cut(s)	50, 70, 73, 82, 85, 369, 1606, 1609, 1777
Bme1390I	CCNGG	9 cut(s)	741, 915, 1267, 1268, 1873, 1887, 1982, 2006, 2059
Bme18I	GGWCC	1 cut(s)	561
BmeT110I	CYCGRG	3 cut(s)	122, 1266, 2205
BmgBI	CACGTC	2 cut(s)	392, 1361
BmgT120I	GGNCC	2 cut(s)	561, 1937
BmiI	GGNNCC	6 cut(s)	75, 181, 313, 1724, 2083, 2233
BmrFI	CCNGG	9 cut(s)	741, 915, 1267, 1268, 1873, 1887, 1982, 2006, 2059
BmrI	ACTGGG	1 cut(s)	1755
BmsI	GCATC	8 cut(s)	813, 1111, 1117, 1271, 1348, 1540, 2163, 2276
BmuI	ACTGGG	1 cut(s)	1755
BpiI	GAAGAC	3 cut(s)	479, 1052, 1097
BplI	GAGNNNNNCTC	2 cut(s)	535, 567
BpmI	CTGGAG	1 cut(s)	2041
Bpu10I	CCTNAGC	1 cut(s)	363
Bpu1102I	GCTNAGC	1 cut(s)	1500
BpuEI	CTTGAG	3 cut(s)	404, 500, 1448
BpuMI	CCSGG	4 cut(s)	1267, 1268, 1982, 2006
Bsa29I	ATCGAT	1 cut(s)	2157
BsaHI	GRCGYC	1 cut(s)	74
BsaJI	CCNNGG	9 cut(s)	712, 1266, 1267, 1885, 1981, 1993, 2005, 2058, 2138
BsaWI	WCCGGW	2 cut(s)	182, 563
BsaXI	ACNNNNNCTCC	4 cut(s)	278, 308, 708, 738
Bsc4I	CCNNNNNNNGG	3 cut(s)	354, 2064, 2262
Bse118I	RCCGGY	3 cut(s)	76, 563, 1939
Bse1I	ACTGG	4 cut(s)	447, 487, 1360, 1750
Bse3DI	GCAATG	4 cut(s)	201, 459, 1169, 1685
BseBI	CCWGG	5 cut(s)	741, 915, 1873, 1887, 2059
BseCI	ATCGAT	1 cut(s)	2157
BseDI	CCNNGG	9 cut(s)	712, 1266, 1267, 1885, 1981, 1993, 2005, 2058, 2138
BseGI	GGATG	6 cut(s)	853, 1262, 1531, 1646, 1682, 2201
BseLI	CCNNNNNNNGG	3 cut(s)	354, 2064, 2262
BseMI	GCAATG	4 cut(s)	201, 459, 1169, 1685
BseMII	CTCAG	7 cut(s)	238, 294, 377, 893, 1155, 1514, 1636
BseNI	ACTGG	4 cut(s)	447, 487, 1360, 1750
BseRI	GAGGAG	6 cut(s)	857, 1423, 1493, 1862, 2001, 2013
BseXI	GCAGC	5 cut(s)	60, 67, 1591, 1594, 1762
BseYI	CCCAGC	1 cut(s)	352
BsgI	GTGCAG	5 cut(s)	67, 423, 627, 1383, 1593
BshFI	GGCC	8 cut(s)	130, 200, 1939, 1992, 2004, 2109, 2181, 2259
BshNI	GGYRCC	2 cut(s)	73, 2081
BshTI	ACCGGT	1 cut(s)	563
BshVI	ATCGAT	1 cut(s)	2157
BsiHKAI	GWGCWC	4 cut(s)	225, 339, 706, 1600
BsiHKCI	CYCGRG	3 cut(s)	122, 1266, 2205
BsiSI	CCGG	8 cut(s)	77, 183, 564, 1267, 1940, 1981, 2005, 2256
BslFI	GGGAC	3 cut(s)	493, 1284, 1639
BslI	CCNNNNNNNGG	3 cut(s)	354, 2064, 2262
BsmAI	GTCTC	6 cut(s)	382, 527, 779, 1138, 1322, 2202
BsmBI	CGTCTC	2 cut(s)	382, 527
BsmFI	GGGAC	3 cut(s)	493, 1284, 1639
BsnI	GGCC	8 cut(s)	130, 200, 1939, 1992, 2004, 2109, 2181, 2259
BsoBI	CYCGRG	3 cut(s)	122, 1266, 2205
Bsp1286I	GDGCHC	5 cut(s)	182, 225, 339, 706, 1600
Bsp1407I	TGTACA	2 cut(s)	57, 1543
Bsp1720I	GCTNAGC	1 cut(s)	1500
BspACI	CCGC	8 cut(s)	68, 71, 83, 315, 367, 559, 1524, 1673
BspANI	GGCC	8 cut(s)	130, 200, 1939, 1992, 2004, 2109, 2181, 2259
BspCNI	CTCAG	7 cut(s)	237, 295, 376, 892, 1156, 1513, 1635
BspDI	ATCGAT	1 cut(s)	2157
BspHI	TCATGA	1 cut(s)	1666
BspLI	GGNNCC	6 cut(s)	75, 181, 313, 1724, 2083, 2233
BspMI	ACCTGC	3 cut(s)	445, 1950, 2184
BspPI	GGATC	5 cut(s)	1013, 1697, 2031, 2041, 2128
BspQI	GCTCTTC	1 cut(s)	58
BspT107I	GGYRCC	2 cut(s)	73, 2081
BsrBI	CCGCTC	1 cut(s)	68
BsrDI	GCAATG	4 cut(s)	201, 459, 1169, 1685
BsrFI	RCCGGY	3 cut(s)	76, 563, 1939
BsrGI	TGTACA	2 cut(s)	57, 1543
BsrI	ACTGG	4 cut(s)	447, 487, 1360, 1750
BssAI	RCCGGY	3 cut(s)	76, 563, 1939
BssECI	CCNNGG	9 cut(s)	712, 1266, 1267, 1885, 1981, 1993, 2005, 2058, 2138
BssNI	GRCGYC	1 cut(s)	74
BssSI	CACGAG	1 cut(s)	465
Bst2BI	CACGAG	1 cut(s)	465
Bst2UI	CCWGG	5 cut(s)	741, 915, 1873, 1887, 2059
Bst4CI	ACNGT	7 cut(s)	143, 661, 773, 1072, 1240, 1548, 2213
Bst6I	CTCTTC	2 cut(s)	58, 225
BstACI	GRCGYC	1 cut(s)	74
BstAPI	GCANNNNNTGC	1 cut(s)	892
BstAUI	TGTACA	2 cut(s)	57, 1543
BstC8I	GCNNGC	8 cut(s)	53, 78, 339, 591, 828, 893, 996, 1920
BstDSI	CCRYGG	2 cut(s)	712, 1993
BstEII	GGTNACC	1 cut(s)	2196
BstF5I	GGATG	6 cut(s)	853, 1262, 1531, 1646, 1682, 2201
BstH2I	RGCGCY	2 cut(s)	77, 2254
BstHHI	GCGC	2 cut(s)	76, 2253
BstMAI	GTCTC	6 cut(s)	382, 527, 779, 1138, 1322, 2202
BstNI	CCWGG	5 cut(s)	741, 915, 1873, 1887, 2059
BstNSI	RCATGY	1 cut(s)	895
BstPI	GGTNACC	1 cut(s)	2196
BstSCI	CCNGG	9 cut(s)	739, 913, 1265, 1266, 1871, 1885, 1980, 2004, 2057
BstSFI	CTRYAG	1 cut(s)	1770
BstV1I	GCAGC	5 cut(s)	60, 67, 1591, 1594, 1762
BstV2I	GAAGAC	3 cut(s)	479, 1052, 1097
BstX2I	RGATCY	3 cut(s)	1702, 1736, 2120
BstYI	RGATCY	3 cut(s)	1702, 1736, 2120
Bsu15I	ATCGAT	1 cut(s)	2157
BsuRI	GGCC	8 cut(s)	130, 200, 1939, 1992, 2004, 2109, 2181, 2259
BsuTUI	ATCGAT	1 cut(s)	2157
BtgI	CCRYGG	2 cut(s)	712, 1993
BtgZI	GCGATG	1 cut(s)	1325
BtrI	CACGTC	2 cut(s)	392, 1361
BtsCI	GGATG	6 cut(s)	853, 1262, 1531, 1646, 1682, 2201
BtsI	GCAGTG	1 cut(s)	103
BveI	ACCTGC	3 cut(s)	445, 1950, 2184
Cac8I	GCNNGC	8 cut(s)	53, 78, 339, 591, 828, 893, 996, 1920
CaiI	CAGNNNCTG	1 cut(s)	1778
CciI	TCATGA	1 cut(s)	1666
CfoI	GCGC	2 cut(s)	76, 2253
Cfr10I	RCCGGY	3 cut(s)	76, 563, 1939
Cfr13I	GGNCC	2 cut(s)	561, 1937
Cfr9I	CCCGGG	1 cut(s)	1266
ClaI	ATCGAT	1 cut(s)	2157
CpoI	CGGWCCG	1 cut(s)	561
CseI	GACGC	1 cut(s)	509
Csp6I	GTAC	6 cut(s)	58, 289, 803, 1353, 1377, 1544
CspAI	ACCGGT	1 cut(s)	563
CspCI	CAANNNNNGTGG	2 cut(s)	1240, 1275
CspI	CGGWCCG	1 cut(s)	561
CviAII	CATG	4 cut(s)	892, 910, 1175, 1667
CviQI	GTAC	6 cut(s)	58, 289, 803, 1353, 1377, 1544
DinI	GGCGCC	1 cut(s)	75
DrdI	GACNNNNNNGTC	1 cut(s)	2142
DseDI	GACNNNNNNGTC	1 cut(s)	2142
EaeI	YGGCCR	3 cut(s)	198, 2107, 2257
Eam1104I	CTCTTC	2 cut(s)	58, 225
EarI	CTCTTC	2 cut(s)	58, 225
EciI	GGCGGA	1 cut(s)	574
Ecl136II	GAGCTC	1 cut(s)	337
Eco147I	AGGCCT	1 cut(s)	130
Eco24I	GRGCYC	2 cut(s)	182, 339
Eco32I	GATATC	2 cut(s)	457, 1908
Eco47I	GGWCC	1 cut(s)	561
Eco53kI	GAGCTC	1 cut(s)	337
Eco57I	CTGAAG	1 cut(s)	1017
Eco88I	CYCGRG	3 cut(s)	122, 1266, 2205
Eco91I	GGTNACC	1 cut(s)	2196
EcoICRI	GAGCTC	1 cut(s)	337
EcoO65I	GGTNACC	1 cut(s)	2196
EcoRII	CCWGG	5 cut(s)	739, 913, 1871, 1885, 2057
EcoRV	GATATC	2 cut(s)	457, 1908
EcoT38I	GRGCYC	2 cut(s)	182, 339
EgeI	GGCGCC	1 cut(s)	75
EheI	GGCGCC	1 cut(s)	75
Esp3I	CGTCTC	2 cut(s)	382, 527
FaeI	CATG	4 cut(s)	895, 913, 1178, 1670
FaqI	GGGAC	3 cut(s)	493, 1284, 1639
FatI	CATG	4 cut(s)	891, 909, 1174, 1666
FauI	CCCGC	1 cut(s)	1666
Fnu4HI	GCNGC	9 cut(s)	49, 69, 72, 81, 84, 368, 1605, 1608, 1776
FokI	GGATG	6 cut(s)	860, 1249, 1518, 1633, 1689, 2188
FriOI	GRGCYC	2 cut(s)	182, 339
Fsp4HI	GCNGC	9 cut(s)	49, 69, 72, 81, 84, 368, 1605, 1608, 1776
FspBI	CTAG	4 cut(s)	576, 1461, 2124, 2296
GlaI	GCGC	2 cut(s)	75, 2252
GluI	GCNGC	9 cut(s)	49, 69, 72, 81, 84, 368, 1605, 1608, 1776
GsaI	CCCAGC	1 cut(s)	356
GsuI	CTGGAG	1 cut(s)	2041
HaeII	RGCGCY	2 cut(s)	77, 2254
HaeIII	GGCC	8 cut(s)	130, 200, 1939, 1992, 2004, 2109, 2181, 2259
HapII	CCGG	8 cut(s)	77, 183, 564, 1267, 1940, 1981, 2005, 2256
HgaI	GACGC	1 cut(s)	509
HhaI	GCGC	2 cut(s)	76, 2253
Hin1I	GRCGYC	1 cut(s)	74
Hin1II	CATG	4 cut(s)	895, 913, 1178, 1670
Hin6I	GCGC	2 cut(s)	74, 2251
HinP1I	GCGC	2 cut(s)	74, 2251
HindIII	AAGCTT	3 cut(s)	869, 992, 2221
HpaII	CCGG	8 cut(s)	77, 183, 564, 1267, 1940, 1981, 2005, 2256
HphI	GGTGA	4 cut(s)	263, 934, 1954, 2190
Hpy166II	GTNNAC	2 cut(s)	1395, 1466
Hpy188III	TCNNGA	9 cut(s)	122, 452, 983, 1427, 1535, 1667, 1844, 2205, 2217
Hpy8I	GTNNAC	2 cut(s)	1395, 1466
Hpy99I	CGWCG	2 cut(s)	94, 332
HpyAV	CCTTC	3 cut(s)	440, 508, 995
HpyCH4III	ACNGT	7 cut(s)	143, 661, 773, 1072, 1240, 1548, 2213
HpyCH4IV	ACGT	3 cut(s)	391, 1360, 1506
HpySE526I	ACGT	3 cut(s)	391, 1360, 1506
Hsp92I	GRCGYC	1 cut(s)	74
Hsp92II	CATG	4 cut(s)	895, 913, 1178, 1670
HspAI	GCGC	2 cut(s)	74, 2251
KasI	GGCGCC	1 cut(s)	73
KroI	GCCGGC	1 cut(s)	76
KroNI	GCCGGC	1 cut(s)	78
LguI	GCTCTTC	1 cut(s)	58
LmnI	GCTCC	4 cut(s)	185, 1826, 2060, 2258
Lsp1109I	GCAGC	5 cut(s)	60, 67, 1591, 1594, 1762
LweI	GCATC	8 cut(s)	813, 1111, 1117, 1271, 1348, 1540, 2163, 2276
MaeI	CTAG	4 cut(s)	576, 1461, 2124, 2296
MaeII	ACGT	3 cut(s)	391, 1360, 1506
MaeIII	GTNAC	5 cut(s)	251, 323, 1741, 1946, 2196
MbiI	CCGCTC	1 cut(s)	68
MboII	GAAGA	8 cut(s)	75, 242, 484, 542, 563, 1057, 1102, 1832
MfeI	CAATTG	2 cut(s)	963, 2242
MflI	RGATCY	3 cut(s)	1702, 1736, 2120
MhlI	GDGCHC	5 cut(s)	182, 225, 339, 706, 1600
MluCI	AATT	8 cut(s)	414, 779, 929, 963, 1232, 1416, 1803, 2242
Mly113I	GGCGCC	1 cut(s)	74
MlyI	GAGTC	8 cut(s)	49, 260, 269, 275, 302, 314, 1499, 2152
MmeI	TCCRAC	6 cut(s)	276, 303, 712, 725, 814, 1768
MroNI	GCCGGC	1 cut(s)	76
MroXI	GAANNNNTTC	2 cut(s)	858, 1818
MseI	TTAA	4 cut(s)	240, 1145, 1211, 1419
MspA1I	CMGCKG	3 cut(s)	317, 367, 1775
MspI	CCGG	8 cut(s)	77, 183, 564, 1267, 1940, 1981, 2005, 2256
MspR9I	CCNGG	9 cut(s)	741, 915, 1267, 1268, 1873, 1887, 1982, 2006, 2059
MunI	CAATTG	2 cut(s)	963, 2242
MvaI	CCWGG	5 cut(s)	741, 915, 1873, 1887, 2059
NaeI	GCCGGC	1 cut(s)	78
NarI	GGCGCC	1 cut(s)	74
NciI	CCSGG	4 cut(s)	1267, 1268, 1982, 2006
NgoMIV	GCCGGC	1 cut(s)	76
NlaIII	CATG	4 cut(s)	895, 913, 1178, 1670
NlaIV	GGNNCC	6 cut(s)	75, 181, 313, 1724, 2083, 2233
NmuCI	GTSAC	5 cut(s)	251, 323, 1741, 1946, 2196
NspI	RCATGY	1 cut(s)	895
PaeI	GCATGC	1 cut(s)	895
PagI	TCATGA	1 cut(s)	1666
PceI	AGGCCT	1 cut(s)	130
PciSI	GCTCTTC	1 cut(s)	58
PcsI	WCGNNNNNNNCGW	1 cut(s)	1348
PdiI	GCCGGC	1 cut(s)	78
PdmI	GAANNNNTTC	2 cut(s)	858, 1818
PflMI	CCANNNNNTGG	1 cut(s)	2064
PinAI	ACCGGT	1 cut(s)	563
PkrI	GCNGC	9 cut(s)	50, 70, 73, 82, 85, 369, 1606, 1609, 1777
PleI	GAGTC	8 cut(s)	48, 260, 269, 275, 301, 313, 1498, 2151
PluTI	GGCGCC	1 cut(s)	77
PpsI	GAGTC	8 cut(s)	48, 260, 269, 275, 301, 313, 1498, 2151
PsiI	TTATAA	1 cut(s)	1067
Psp124BI	GAGCTC	1 cut(s)	339
Psp6I	CCWGG	5 cut(s)	739, 913, 1871, 1885, 2057
PspEI	GGTNACC	1 cut(s)	2196
PspFI	CCCAGC	1 cut(s)	352
PspGI	CCWGG	5 cut(s)	739, 913, 1871, 1885, 2057
PspN4I	GGNNCC	6 cut(s)	75, 181, 313, 1724, 2083, 2233
PspPI	GGNCC	2 cut(s)	561, 1937
PstNI	CAGNNNCTG	1 cut(s)	1778
PsuI	RGATCY	3 cut(s)	1702, 1736, 2120
PvuII	CAGCTG	1 cut(s)	1775
RsaI	GTAC	6 cut(s)	59, 290, 804, 1354, 1378, 1545
RsaNI	GTAC	6 cut(s)	58, 289, 803, 1353, 1377, 1544
Rsr2I	CGGWCCG	1 cut(s)	561
RsrII	CGGWCCG	1 cut(s)	561
SacI	GAGCTC	1 cut(s)	339
SapI	GCTCTTC	1 cut(s)	58
SaqAI	TTAA	4 cut(s)	240, 1145, 1211, 1419
SatI	GCNGC	9 cut(s)	49, 69, 72, 81, 84, 368, 1605, 1608, 1776
Sau96I	GGNCC	2 cut(s)	561, 1937
SchI	GAGTC	8 cut(s)	49, 260, 269, 275, 302, 314, 1499, 2152
ScrFI	CCNGG	9 cut(s)	741, 915, 1267, 1268, 1873, 1887, 1982, 2006, 2059
SduI	GDGCHC	5 cut(s)	182, 225, 339, 706, 1600
SfaNI	GCATC	8 cut(s)	813, 1111, 1117, 1271, 1348, 1540, 2163, 2276
SfcI	CTRYAG	1 cut(s)	1770
SfoI	GGCGCC	1 cut(s)	75
SinI	GGWCC	1 cut(s)	561
SmaI	CCCGGG	1 cut(s)	1268
SmlI	CTYRAG	3 cut(s)	383, 515, 1427
SmoI	CTYRAG	3 cut(s)	383, 515, 1427
SphI	GCATGC	1 cut(s)	895
Sse9I	AATT	8 cut(s)	414, 779, 929, 963, 1232, 1416, 1803, 2242
SseBI	AGGCCT	1 cut(s)	130
SsiI	CCGC	8 cut(s)	68, 71, 83, 315, 367, 559, 1524, 1673
SspDI	GGCGCC	1 cut(s)	73
SspI	AATATT	2 cut(s)	797, 944
SspMI	CTAG	4 cut(s)	576, 1461, 2124, 2296
SstI	GAGCTC	1 cut(s)	339
StuI	AGGCCT	1 cut(s)	130
StyD4I	CCNGG	9 cut(s)	739, 913, 1265, 1266, 1871, 1885, 1980, 2004, 2057
TaaI	ACNGT	7 cut(s)	143, 661, 773, 1072, 1240, 1548, 2213
TaiI	ACGT	3 cut(s)	394, 1363, 1509
TaqI	TCGA	5 cut(s)	401, 451, 821, 1290, 2157
TasI	AATT	8 cut(s)	414, 779, 929, 963, 1232, 1416, 1803, 2242
TatI	WGTACW	2 cut(s)	57, 1543
TauI	GCSGC	4 cut(s)	71, 74, 86, 370
Tru1I	TTAA	4 cut(s)	240, 1145, 1211, 1419
Tru9I	TTAA	4 cut(s)	240, 1145, 1211, 1419
TseFI	GTSAC	5 cut(s)	251, 323, 1741, 1946, 2196
TseI	GCWGC	5 cut(s)	48, 80, 1604, 1607, 1775
Tsp45I	GTSAC	5 cut(s)	251, 323, 1741, 1946, 2196
TspDTI	ATGAA	7 cut(s)	552, 647, 825, 1596, 1797, 1827, 2082
TspGWI	ACGGA	3 cut(s)	33, 952, 1831
TspMI	CCCGGG	1 cut(s)	1266
Van91I	CCANNNNNTGG	1 cut(s)	2064
VpaK11BI	GGWCC	1 cut(s)	561
XapI	RAATTY	1 cut(s)	1232
XceI	RCATGY	1 cut(s)	895
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1291
XmaI	CCCGGG	1 cut(s)	1266
XmnI	GAANNNNTTC	2 cut(s)	858, 1818
XspI	CTAG	4 cut(s)	576, 1461, 2124, 2296

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.