Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr5

Orthogroup ID

OG0008791

Physical Location & Seq

Genomic Coordinates Reverse (-)

42088909 .. 42094518

Gene Structure 5610 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000025121

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2286 bp

ATGGCGTCGATTATCGGAGTTTCCTCTATATACTACCAGACTCCATCTGAGTTGTATAAAAGAGCTGCCGCGCCTGTAGCTACCACGACGGCCCCGACGACGACGAGCAGTAGCGGCGGCGGCGGCGGCGTTTCGTTGGCTTTTCCGGAGAGGCAGAGGGCTCACTTCAACAGTGTTTTGAGAGCGAGGAGTGTTTTCGCTACTCAGGCGATTGCGACGCCTAATTCTGTGCTCAGTGAGGAGGCCTTCAAGGGGCTAGGGGACTTTTCGAAGGGGGAGCTGGACTCGGAGTCGGAGTATGAGTCGGAGAGTGATGTGGTTGAGCCGGTTAGTAAAGACGAGCTGGCTCTTTCGAAATTGGGCCTGCCTCAGCGGCTTGTGGATAGTCTTGAGAGGCGTGGCATTTCTTCGCTTTTTCCCATTCAGAGAGCTGTGCTAGTGCCGGCACTGGAAGGTCGAGACATCATAGCTCGTGCAAAGACTGGGACTGGGAAGACATTGGCCTTTGGGATTCCCATACTTAAACGCCTTACAGAAGATGATGCACAGAGAGGTTCAAGGCGGTCTGGTTATCTTCCTAGAGTTCTGGTCCTTGCACCTACTCGGGAGTTAGCGAAGCAAGTGGACAAGGAGATTAAAGAATCTGCACCTTATCTGAACACTGTTTGCGTCTATGGGGGAGTTTCATACATAACACAACAGAGTGCTCTTTCTCGCGGGGTTGATGTAGTGGTTGGAACTCCTGGTCGGCTTATTGACCTGATAAACGGCGGCAGCCTCAGGTTGGGTGAAGTTCAATATTTGGTACTTGATGAAGCTGATCAGATGCTTGCTGTTGGATTTGAGGAAGATGTGGAAACAATTCTACAAAGGCTCCCAGCTCAGAGGCAGAGCATGCTTTTTTCTGCAACTATGCCCACTTGGGTGAAAAAACTAGCACGAAAATATTTGGACAATCCATTGACGATTGATTTGGTTGGTGATCAAGATGAGAAGCTTGCAGAAGGGATCAAACTCTATGCTCTATCAACCACAAACTCTTCAAAGCGGACTATTCTTAGTGATCTTATAACAGTTTATGCAAAGGGTGGGAAGACCATTGTTTTTACACAAACGAAACGTGATGCTGATGAAGTGTCAATGTCATTAACGACTAGCATAGCTTCTGAGGCATTGCATGGAGATATATCACAGCATCAAAGGGAGAGAACCCTTAATGGTTTTCGGCAAGGAAAATTCACTGTGCTCGTTGCCACTGATGTTGCATCCCGTGGACTTGATATTCCCAATGTTGACTTGGTTATCCACTATGAACTTCCCAATGATTCAGAGACATTTGTACATCGCTCTGGTCGTACTGGACGTGCAGGGAAACTCGGTAATGCCGTTCTGATGTTTACTAATAACCAGAGGAGAACAGTCAAAACCCTTGAGCGTGATGTGGGGTGCAGGTTTGAGTTTGTTACTCCGCCAACCGTTGAAGAGGTTTTGGAGTCATCTGCTTCACATGTGGTTGCTACTCTCAATGGGGTTCATCCTGAATCCATACAGTTTTTTACACCGACGGCACAGAGGCTGATTGAAGAACAGGGAACAACTGCTCTTGCTGCTGCGCTGGCACAGCTAAGTGGATTTTCCCGACCTCCGTCATCCCGGTCTCTAATCAGTCATGAGCCGGGGTGGACGACATTGCAAATAATTCGGGATCCAGCTTTTGCCAGAGGTTACCTGTCAGCTAGATCTGTCACTGGGTTTCTTTCAGATGTTTATTCTGCAGCTGCTGATGAACTTGGAAAAATATATTTAATTGCAGATGAAAGGGTTCAAGGAGCAGTTTTTGATCTTCCAGAGGAGATTGCAAAAGAGTTGCTGAAGAGGCAAATACCTCCTGGAAACAGTATTTCCAAGATCAGCAAGTTGCCTCCTCTGCAAGATGACGGGCCAGTGAGTGACAACTATGGCAGGTTTTCAGCTAGGGATCGGAGTAGCCGACGGGGAAGTTTTAGTAGTGGACGGGGAGGTTTCAGAAGTAGCTCTAGGGGTGGATGGGGAGGCGGCCGAGGTTCTAGTGATGGTGATGATGATTTTAGGAGTAGCAGAGGAAGTTTTAATAGATCTAGCAGCAGCCGACCTCGGATGACAAGAAGTATGGATGATGATTGGCTTATTGGAGGTACACGAACAGGCAGAATACCACCAAGAGACAGAAGCTTTGGAGGTTCCTGTTTCAATTGTGGGCAGTCTGGACACAGGGCATCAGAGTGTCCTCAGAAACAAGGATATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000373 GO:0000375 GO:0000377 GO:0003674 GO:0003676 GO:0003723 GO:0003724 GO:0003729 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0006970 GO:0006996 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0008380 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009628 GO:0009651 GO:0009657 GO:0009658 GO:0009941 GO:0009987 GO:0010467 GO:0010501 GO:0016020 GO:0016043 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0022613 GO:0031967 GO:0031974 GO:0031975 GO:0031981 GO:0034641 GO:0042254 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0050896 GO:0070013 GO:0070035 GO:0071704 GO:0071840 GO:0090304 GO:0097159 GO:0140098 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

ko01110 map01110

Pfam Domains

Protein Families

DEAD GUCT Helicase_C zf-CCHC

Protein Analysis

762

Amino Acids

82.59

Weight (kDa)

8.71

Isoelectric Point (pI)

46.5

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	140 - 311	2.2e-45	DEAD/DEAH box helicase
ResIII	PF04851	153 - 305	8e-06	Type III restriction enzyme, res subunit
Helicase_C	PF00271	349 - 457	8e-32	Helicase conserved C-terminal domain
DD_DDX21-DDX50	PF26142	492 - 541	1.1e-16	DDX21/DDX50 dimerisation domain
GUCT	PF08152	544 - 644	1.9e-14	GUCT (NUC152) domain
zf-CCHC	PF00098	741 - 757	1.2e-06	Zinc knuckle

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0008791)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G26742 AT5G26742 AT5G26742
fragaria_vesca	FvH4_6g08810 FvH4_6g08810 FvH4_6g08810 FvH4_6g08810
malus_domestica	MD04G1183400.v1.1 MD12G1196800.v1.1
prunus_persica	Prupe.6G301900_v2.0.a1
pyrus_communis	pycom04g16190 pycom12g18300
rosa_chinensis	RchiOBHm_Chr3g0459081
rosa_laevigata	RLG00000025121
rosa_multiflora	Rmu_sc0000466.1_g000002 Rmu_sc0003515.1_g000001
rosa_roxburghii	Rroxscaffold_6G00420530
rosa_rugosa	Rorug03G0033400
rosa_samantha	Rh3AG091300 Rh3BG094400 Rh3CG094800 Rh3DG094800
rosa_wichuraiana	Rw3G007840

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1070
Acc36I	ACCTGC	2 cut(s)	1440, 1953
AccII	CGCG	2 cut(s)	71, 717
AccIII	TCCGGA	1 cut(s)	145
AclWI	GGATC	4 cut(s)	1016, 1700, 1713, 1986
AcoI	YGGCCR	1 cut(s)	2056
AcsI	RAATTY	1 cut(s)	1235
AcuI	CTGAAG	1 cut(s)	1892
AcyI	GRCGYC	2 cut(s)	5, 218
AfaI	GTAC	4 cut(s)	807, 1341, 1357, 2176
AfiI	CCNNNNNNNGG	2 cut(s)	252, 784
AflIII	ACRYGT	1 cut(s)	1507
AgsI	TTSAA	9 cut(s)	169, 250, 558, 797, 1044, 1481, 1583, 1826, 2230
AhdI	GACNNNNNGTC	1 cut(s)	289
AjiI	CACGTC	1 cut(s)	1364
AjnI	CCWGG	2 cut(s)	742, 1888
AleI	CACNNNNGTG	1 cut(s)	923
Alw21I	GWGCWC	3 cut(s)	234, 709, 1248
Alw26I	GTCTC	4 cut(s)	453, 1325, 1662, 2196
AlwI	GGATC	4 cut(s)	1016, 1700, 1713, 1986
AlwNI	CAGNNNCTG	2 cut(s)	1576, 1781
Ama87I	CYCGRG	1 cut(s)	603
Aor13HI	TCCGGA	1 cut(s)	145
AoxI	GGCC	6 cut(s)	90, 243, 361, 501, 1940, 2056
ApeKI	GCWGC	8 cut(s)	65, 774, 1607, 1610, 1775, 1778, 2121, 2124
ApoI	RAATTY	1 cut(s)	1235
ArsI	GACNNNNNNTTYG	4 cut(s)	955, 987, 2195, 2227
Asp700I	GAANNNNTTC	2 cut(s)	861, 1821
AspLEI	GCGC	2 cut(s)	73, 1615
AspS9I	GGNCC	4 cut(s)	91, 361, 589, 1940
AsuC2I	CCSGG	2 cut(s)	1654, 1677
AsuHPI	GGTGA	4 cut(s)	800, 937, 992, 2087
AsuII	TTCGAA	2 cut(s)	269, 353
AvaI	CYCGRG	1 cut(s)	603
AvaII	GGWCC	1 cut(s)	589
AxyI	CCTNAGG	1 cut(s)	779
BamHI	GGATCC	1 cut(s)	1705
BanII	GRGCYC	1 cut(s)	163
BauI	CACGAG	1 cut(s)	471
BbsI	GAAGAC	2 cut(s)	500, 1100
Bbv12I	GWGCWC	3 cut(s)	234, 709, 1248
BbvCI	CCTCAGC	1 cut(s)	369
BbvI	GCAGC	8 cut(s)	52, 786, 1594, 1597, 1765, 1787, 2133, 2136
BccI	CCATC	3 cut(s)	52, 2040, 2066
BceAI	ACGGC	4 cut(s)	105, 784, 1370, 1581
BcgI	CGANNNNNNTGC	2 cut(s)	1682, 1716
BciT130I	CCWGG	2 cut(s)	744, 1890
BclI	TGATCA	2 cut(s)	820, 982
BcnI	CCSGG	2 cut(s)	1654, 1677
BcoDI	GTCTC	4 cut(s)	453, 1325, 1662, 2196
BfmI	CTRYAG	2 cut(s)	75, 1773
BfuAI	ACCTGC	2 cut(s)	1440, 1953
BglI	GCCNNNNNGGC	1 cut(s)	373
BglII	AGATCT	2 cut(s)	1739, 2114
Bme1390I	CCNGG	4 cut(s)	744, 1654, 1677, 1890
Bme18I	GGWCC	1 cut(s)	589
BmeRI	GACNNNNNGTC	1 cut(s)	289
BmeT110I	CYCGRG	1 cut(s)	603
BmgBI	CACGTC	1 cut(s)	1364
BmgT120I	GGNCC	4 cut(s)	91, 361, 589, 1940
BmiI	GGNNCC	4 cut(s)	93, 875, 1707, 2221
BmrFI	CCNGG	4 cut(s)	744, 1654, 1677, 1890
BmrI	ACTGGG	3 cut(s)	492, 498, 1758
BmsI	GCATC	6 cut(s)	532, 816, 1114, 1204, 1274, 2264
BmuI	ACTGGG	3 cut(s)	492, 498, 1758
BoxI	GACNNNNGTC	1 cut(s)	1645
BpiI	GAAGAC	2 cut(s)	500, 1100
BplI	GAGNNNNNCTC	2 cut(s)	269, 301
Bpu10I	CCTNAGC	1 cut(s)	369
Bpu14I	TTCGAA	2 cut(s)	269, 353
BpuEI	CTTGAG	2 cut(s)	410, 1451
BpuMI	CCSGG	2 cut(s)	1654, 1677
BsaHI	GRCGYC	2 cut(s)	5, 218
BsaI	GGTCTC	1 cut(s)	1662
BsaJI	CCNNGG	4 cut(s)	1270, 1676, 2059, 2132
BsaWI	WCCGGW	1 cut(s)	145
BsaXI	ACNNNNNCTCC	6 cut(s)	281, 287, 311, 317, 858, 888
Bsc4I	CCNNNNNNNGG	2 cut(s)	252, 784
Bse118I	RCCGGY	2 cut(s)	325, 442
Bse1I	ACTGG	6 cut(s)	453, 487, 493, 1363, 1753, 1943
Bse21I	CCTNAGG	1 cut(s)	779
Bse3DI	GCAATG	2 cut(s)	1172, 1688
BseAI	TCCGGA	1 cut(s)	145
BseBI	CCWGG	2 cut(s)	744, 1890
BseDI	CCNNGG	4 cut(s)	1270, 1676, 2059, 2132
BseGI	GGATG	6 cut(s)	1265, 1534, 1649, 2051, 2142, 2158
BseLI	CCNNNNNNNGG	2 cut(s)	252, 784
BseMI	GCAATG	2 cut(s)	1172, 1688
BseMII	CTCAG	8 cut(s)	39, 218, 247, 383, 793, 896, 1158, 2282
BseNI	ACTGG	6 cut(s)	453, 487, 493, 1363, 1753, 1943
BseRI	GAGGAG	5 cut(s)	202, 254, 1426, 1865, 1914
BseX3I	CGGCCG	1 cut(s)	2056
BseXI	GCAGC	8 cut(s)	52, 786, 1594, 1597, 1765, 1787, 2133, 2136
BseYI	CCCAGC	1 cut(s)	877
BsgI	GTGCAG	3 cut(s)	630, 1386, 1468
Bsh1236I	CGCG	2 cut(s)	71, 717
Bsh1285I	CGRYCG	1 cut(s)	2059
BshFI	GGCC	6 cut(s)	92, 245, 363, 503, 1942, 2058
BsiEI	CGRYCG	1 cut(s)	2059
BsiHKAI	GWGCWC	3 cut(s)	234, 709, 1248
BsiHKCI	CYCGRG	1 cut(s)	603
BsiSI	CCGG	5 cut(s)	146, 326, 443, 1654, 1676
BslFI	GGGAC	2 cut(s)	275, 499
BslI	CCNNNNNNNGG	2 cut(s)	252, 784
BsmAI	GTCTC	4 cut(s)	453, 1325, 1662, 2196
BsmFI	GGGAC	2 cut(s)	275, 499
BsnI	GGCC	6 cut(s)	92, 245, 363, 503, 1942, 2058
Bso31I	GGTCTC	1 cut(s)	1662
BsoBI	CYCGRG	1 cut(s)	603
Bsp119I	TTCGAA	2 cut(s)	269, 353
Bsp1286I	GDGCHC	4 cut(s)	163, 234, 709, 1248
Bsp13I	TCCGGA	1 cut(s)	145
Bsp1407I	TGTACA	1 cut(s)	1339
BspANI	GGCC	6 cut(s)	92, 245, 363, 503, 1942, 2058
BspCNI	CTCAG	8 cut(s)	40, 217, 246, 382, 792, 895, 1159, 2281
BspEI	TCCGGA	1 cut(s)	145
BspFNI	CGCG	2 cut(s)	71, 717
BspHI	TCATGA	1 cut(s)	1669
BspLI	GGNNCC	4 cut(s)	93, 875, 1707, 2221
BspMAI	CTGCAG	1 cut(s)	1777
BspMI	ACCTGC	2 cut(s)	1440, 1953
BspPI	GGATC	4 cut(s)	1016, 1700, 1713, 1986
BspT104I	TTCGAA	2 cut(s)	269, 353
BspTNI	GGTCTC	1 cut(s)	1662
BsrDI	GCAATG	2 cut(s)	1172, 1688
BsrFI	RCCGGY	2 cut(s)	325, 442
BsrGI	TGTACA	1 cut(s)	1339
BsrI	ACTGG	6 cut(s)	453, 487, 493, 1363, 1753, 1943
BssAI	RCCGGY	2 cut(s)	325, 442
BssECI	CCNNGG	4 cut(s)	1270, 1676, 2059, 2132
BssNI	GRCGYC	2 cut(s)	5, 218
BssSI	CACGAG	1 cut(s)	471
Bst2BI	CACGAG	1 cut(s)	471
Bst2UI	CCWGG	2 cut(s)	744, 1890
Bst4CI	ACNGT	8 cut(s)	173, 664, 1075, 1243, 1420, 1477, 1551, 1898
Bst6I	CTCTTC	3 cut(s)	1045, 1476, 1868
BstACI	GRCGYC	2 cut(s)	5, 218
BstAPI	GCANNNNNTGC	1 cut(s)	895
BstAUI	TGTACA	1 cut(s)	1339
BstBI	TTCGAA	2 cut(s)	269, 353
BstC8I	GCNNGC	7 cut(s)	345, 365, 444, 831, 896, 999, 1617
BstDSI	CCRYGG	1 cut(s)	1270
BstEII	GGTNACC	1 cut(s)	1724
BstF5I	GGATG	6 cut(s)	1265, 1534, 1649, 2051, 2142, 2158
BstFNI	CGCG	2 cut(s)	71, 717
BstHHI	GCGC	2 cut(s)	73, 1615
BstMAI	GTCTC	4 cut(s)	453, 1325, 1662, 2196
BstMCI	CGRYCG	1 cut(s)	2059
BstNI	CCWGG	2 cut(s)	744, 1890
BstNSI	RCATGY	2 cut(s)	898, 1511
BstPAI	GACNNNNGTC	1 cut(s)	1645
BstPI	GGTNACC	1 cut(s)	1724
BstSCI	CCNGG	4 cut(s)	742, 1652, 1675, 1888
BstSFI	CTRYAG	2 cut(s)	75, 1773
BstUI	CGCG	2 cut(s)	71, 717
BstV1I	GCAGC	8 cut(s)	52, 786, 1594, 1597, 1765, 1787, 2133, 2136
BstV2I	GAAGAC	2 cut(s)	500, 1100
BstX2I	RGATCY	3 cut(s)	1705, 1739, 2114
BstYI	RGATCY	3 cut(s)	1705, 1739, 2114
BstZI	CGGCCG	1 cut(s)	2056
Bsu36I	CCTNAGG	1 cut(s)	779
BsuRI	GGCC	6 cut(s)	92, 245, 363, 503, 1942, 2058
BtgI	CCRYGG	1 cut(s)	1270
BtgZI	GCGATG	1 cut(s)	1328
BtrI	CACGTC	1 cut(s)	1364
BtsCI	GGATG	6 cut(s)	1265, 1534, 1649, 2051, 2142, 2158
BtsIMutI	CAGTG	8 cut(s)	178, 241, 446, 660, 1239, 1254, 1746, 1950
BveI	ACCTGC	2 cut(s)	1440, 1953
Cac8I	GCNNGC	7 cut(s)	345, 365, 444, 831, 896, 999, 1617
CaiI	CAGNNNCTG	2 cut(s)	1576, 1781
CciI	TCATGA	1 cut(s)	1669
CfoI	GCGC	2 cut(s)	73, 1615
Cfr10I	RCCGGY	2 cut(s)	325, 442
Cfr13I	GGNCC	4 cut(s)	91, 361, 589, 1940
CseI	GACGC	2 cut(s)	226, 658
Csp6I	GTAC	4 cut(s)	806, 1340, 1356, 2175
CspCI	CAANNNNNGTGG	2 cut(s)	1243, 1278
CviAII	CATG	4 cut(s)	895, 1178, 1508, 1670
CviQI	GTAC	4 cut(s)	806, 1340, 1356, 2175
DriI	GACNNNNNGTC	1 cut(s)	289
EaeI	YGGCCR	1 cut(s)	2056
EagI	CGGCCG	1 cut(s)	2056
Eam1104I	CTCTTC	3 cut(s)	1045, 1476, 1868
Eam1105I	GACNNNNNGTC	1 cut(s)	289
EarI	CTCTTC	3 cut(s)	1045, 1476, 1868
EciI	GGCGGA	1 cut(s)	1458
EclXI	CGGCCG	1 cut(s)	2056
Eco147I	AGGCCT	1 cut(s)	245
Eco24I	GRGCYC	1 cut(s)	163
Eco31I	GGTCTC	1 cut(s)	1662
Eco47I	GGWCC	1 cut(s)	589
Eco52I	CGGCCG	1 cut(s)	2056
Eco57I	CTGAAG	1 cut(s)	1892
Eco81I	CCTNAGG	1 cut(s)	779
Eco88I	CYCGRG	1 cut(s)	603
Eco91I	GGTNACC	1 cut(s)	1724
EcoO65I	GGTNACC	1 cut(s)	1724
EcoRII	CCWGG	2 cut(s)	742, 1888
EcoT38I	GRGCYC	1 cut(s)	163
FaeI	CATG	4 cut(s)	898, 1181, 1511, 1673
FaqI	GGGAC	2 cut(s)	275, 499
FatI	CATG	4 cut(s)	894, 1177, 1507, 1669
FauI	CCCGC	1 cut(s)	710
FbaI	TGATCA	2 cut(s)	820, 982
FokI	GGATG	6 cut(s)	1252, 1521, 1636, 2058, 2149, 2165
FriOI	GRGCYC	1 cut(s)	163
GlaI	GCGC	2 cut(s)	72, 1614
GsaI	CCCAGC	1 cut(s)	881
HaeIII	GGCC	6 cut(s)	92, 245, 363, 503, 1942, 2058
HapII	CCGG	5 cut(s)	146, 326, 443, 1654, 1676
HgaI	GACGC	2 cut(s)	226, 658
HhaI	GCGC	2 cut(s)	73, 1615
Hin1I	GRCGYC	2 cut(s)	5, 218
Hin1II	CATG	4 cut(s)	898, 1181, 1511, 1673
Hin6I	GCGC	2 cut(s)	71, 1613
HinP1I	GCGC	2 cut(s)	71, 1613
HincII	GTYRAC	1 cut(s)	1294
HindII	GTYRAC	1 cut(s)	1294
HindIII	AAGCTT	2 cut(s)	995, 2209
HinfI	GANTC	9 cut(s)	40, 284, 290, 302, 511, 641, 1325, 1493, 1541
HpaII	CCGG	5 cut(s)	146, 326, 443, 1654, 1676
HphI	GGTGA	4 cut(s)	800, 937, 992, 2087
Hpy166II	GTNNAC	7 cut(s)	625, 1274, 1294, 1398, 1683, 2012, 2177
Hpy8I	GTNNAC	7 cut(s)	625, 1274, 1294, 1398, 1683, 2012, 2177
Hpy99I	CGWCG	8 cut(s)	10, 91, 100, 103, 106, 220, 1567, 1995
HpyAV	CCTTC	4 cut(s)	256, 265, 446, 998
HpyCH4III	ACNGT	8 cut(s)	173, 664, 1075, 1243, 1420, 1477, 1551, 1898
HpyCH4IV	ACGT	2 cut(s)	1120, 1363
HpySE526I	ACGT	2 cut(s)	1120, 1363
Hsp92I	GRCGYC	2 cut(s)	5, 218
Hsp92II	CATG	4 cut(s)	898, 1181, 1511, 1673
HspAI	GCGC	2 cut(s)	71, 1613
Kpn2I	TCCGGA	1 cut(s)	145
KroI	GCCGGC	1 cut(s)	442
KroNI	GCCGGC	1 cut(s)	444
Ksp22I	TGATCA	2 cut(s)	820, 982
LmnI	GCTCC	3 cut(s)	277, 879, 1829
Lsp1109I	GCAGC	8 cut(s)	52, 786, 1594, 1597, 1765, 1787, 2133, 2136
LweI	GCATC	6 cut(s)	532, 816, 1114, 1204, 1274, 2264
MaeII	ACGT	2 cut(s)	1120, 1363
MaeIII	GTNAC	4 cut(s)	1462, 1724, 1744, 1949
MfeI	CAATTG	1 cut(s)	2230
MflI	RGATCY	3 cut(s)	1705, 1739, 2114
MhlI	GDGCHC	4 cut(s)	163, 234, 709, 1248
MluCI	AATT	7 cut(s)	223, 356, 861, 1235, 1698, 1806, 2230
MlyI	GAGTC	5 cut(s)	34, 278, 299, 311, 1502
MmeI	TCCRAC	4 cut(s)	273, 285, 715, 817
MroI	TCCGGA	1 cut(s)	145
MroNI	GCCGGC	1 cut(s)	442
MroXI	GAANNNNTTC	2 cut(s)	861, 1821
MseI	TTAA	6 cut(s)	522, 636, 1148, 1215, 1805, 2109
MslI	CAYNNNNRTG	2 cut(s)	923, 2260
MspA1I	CMGCKG	2 cut(s)	373, 1778
MspI	CCGG	5 cut(s)	146, 326, 443, 1654, 1676
MspR9I	CCNGG	4 cut(s)	744, 1654, 1677, 1890
MunI	CAATTG	1 cut(s)	2230
MvaI	CCWGG	2 cut(s)	744, 1890
MvnI	CGCG	2 cut(s)	71, 717
NaeI	GCCGGC	1 cut(s)	444
NciI	CCSGG	2 cut(s)	1654, 1677
NgoMIV	GCCGGC	1 cut(s)	442
NlaIII	CATG	4 cut(s)	898, 1181, 1511, 1673
NlaIV	GGNNCC	4 cut(s)	93, 875, 1707, 2221
NmeAIII	GCCGAG	1 cut(s)	2084
NmuCI	GTSAC	2 cut(s)	1744, 1949
NspI	RCATGY	2 cut(s)	898, 1511
NspV	TTCGAA	2 cut(s)	269, 353
OliI	CACNNNNGTG	1 cut(s)	923
PaeI	GCATGC	1 cut(s)	898
PagI	TCATGA	1 cut(s)	1669
PceI	AGGCCT	1 cut(s)	245
PciI	ACATGT	1 cut(s)	1507
PcsI	WCGNNNNNNNCGW	6 cut(s)	92, 95, 1351, 1360, 1383, 1987
PdiI	GCCGGC	1 cut(s)	444
PdmI	GAANNNNTTC	2 cut(s)	861, 1821
PfeI	GAWTC	4 cut(s)	511, 641, 1325, 1541
PfoI	TCCNGGA	1 cut(s)	1888
PleI	GAGTC	5 cut(s)	34, 278, 298, 310, 1501
PpsI	GAGTC	5 cut(s)	34, 278, 298, 310, 1501
PscI	ACATGT	1 cut(s)	1507
PshAI	GACNNNNGTC	1 cut(s)	1645
PsiI	TTATAA	1 cut(s)	1070
Psp6I	CCWGG	2 cut(s)	742, 1888
PspEI	GGTNACC	1 cut(s)	1724
PspFI	CCCAGC	1 cut(s)	877
PspGI	CCWGG	2 cut(s)	742, 1888
PspN4I	GGNNCC	4 cut(s)	93, 875, 1707, 2221
PspPI	GGNCC	4 cut(s)	91, 361, 589, 1940
PstI	CTGCAG	1 cut(s)	1777
PstNI	CAGNNNCTG	2 cut(s)	1576, 1781
PsuI	RGATCY	3 cut(s)	1705, 1739, 2114
PvuII	CAGCTG	1 cut(s)	1778
RsaI	GTAC	4 cut(s)	807, 1341, 1357, 2176
RsaNI	GTAC	4 cut(s)	806, 1340, 1356, 2175
RseI	CAYNNNNRTG	2 cut(s)	923, 2260
SaqAI	TTAA	6 cut(s)	522, 636, 1148, 1215, 1805, 2109
Sau96I	GGNCC	4 cut(s)	91, 361, 589, 1940
SchI	GAGTC	5 cut(s)	34, 278, 299, 311, 1502
ScrFI	CCNGG	4 cut(s)	744, 1654, 1677, 1890
SduI	GDGCHC	4 cut(s)	163, 234, 709, 1248
SfaNI	GCATC	6 cut(s)	532, 816, 1114, 1204, 1274, 2264
SfcI	CTRYAG	2 cut(s)	75, 1773
SfuI	TTCGAA	2 cut(s)	269, 353
SinI	GGWCC	1 cut(s)	589
SmiMI	CAYNNNNRTG	2 cut(s)	923, 2260
SmlI	CTYRAG	2 cut(s)	389, 1430
SmoI	CTYRAG	2 cut(s)	389, 1430
SphI	GCATGC	1 cut(s)	898
Sse9I	AATT	7 cut(s)	223, 356, 861, 1235, 1698, 1806, 2230
SseBI	AGGCCT	1 cut(s)	245
SspI	AATATT	2 cut(s)	800, 947
StuI	AGGCCT	1 cut(s)	245
StyD4I	CCNGG	4 cut(s)	742, 1652, 1675, 1888
TaaI	ACNGT	8 cut(s)	173, 664, 1075, 1243, 1420, 1477, 1551, 1898
TaiI	ACGT	2 cut(s)	1123, 1366
TaqI	TCGA	4 cut(s)	8, 269, 353, 457
TasI	AATT	7 cut(s)	223, 356, 861, 1235, 1698, 1806, 2230
TatI	WGTACW	1 cut(s)	1339
TauI	GCSGC	9 cut(s)	71, 117, 120, 123, 126, 129, 376, 774, 2058
TfiI	GAWTC	4 cut(s)	511, 641, 1325, 1541
Tru1I	TTAA	6 cut(s)	522, 636, 1148, 1215, 1805, 2109
Tru9I	TTAA	6 cut(s)	522, 636, 1148, 1215, 1805, 2109
TscAI	CASTG	8 cut(s)	178, 241, 453, 667, 1246, 1261, 1753, 1950
TseFI	GTSAC	2 cut(s)	1744, 1949
TseI	GCWGC	8 cut(s)	65, 774, 1607, 1610, 1775, 1778, 2121, 2124
Tsp45I	GTSAC	2 cut(s)	1744, 1949
TspDTI	ATGAA	7 cut(s)	675, 828, 1146, 1326, 1523, 1800, 1830
TspGWI	ACGGA	1 cut(s)	1635
TspRI	CASTG	8 cut(s)	178, 241, 453, 667, 1246, 1261, 1753, 1950
VpaK11BI	GGWCC	1 cut(s)	589
XapI	RAATTY	1 cut(s)	1235
XceI	RCATGY	2 cut(s)	898, 1511
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1294
XmnI	GAANNNNTTC	2 cut(s)	861, 1821

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.