FvH4_6g09070

Bifunctional protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0006558
Physical Location & Seq
Forward (+)
5368744 .. 5372337
3594 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g09070.t2

Sequence Viewer

Length: 660 bp
ATGTGGCTCACCTGCCAGAGCATTGTACAAAAGATCAGCTTCTTAAAGCTTTGTCAAAGTTTGATGTGGATCCCACTGTTCATGCATTTGGATGAGAGGAAGATTATGGATGTGCTGAGTCCAGAGAAAGACGTGGATGGCTTCCATCCAATTAATATCGGAAATCTGGCCATGAGAGGAAGGGAGCCTATGTTTATTCCGTGCACTCCAAAGGGTTGCATTGAGTTGTTGCTCAGGTCTGGTGTGGAAATAGTTGGGAAGAAAGCTGCTGTCATTGGCAGAAGTAATATTGTTGGATTGCCCGCATCATTGCTTTTGCAGAGACACCATGCAACAGTCAGTATCGTACATGCGTTCACAAAGAACCCAGAACATATCACCTGTGAAGCTGACATTGTGGTCACAGCTGCAGGAGTGCCTAATCTTGTCCGTGGAAATTGGCTAAAACCTGGAGCAGTTGTCATTGATGTTGGGACCAATCCAGTTGAGGACCCTAGCTGTGAGCGAGGTTATCGTCTCATAGGAGATGTTTGCTATGAAGAAGCAGTGCGGGTGGTATCAGCCATTACCCCAGTTCCTGGAGGTGTTGGACCTATGACAATTGCTATGCTTCTATCAAACACACTTGATTCAGCAAAGCGAGCCTATGCCTTTACCTGA

Protein Analysis

220

Amino Acids

23.84

Weight (kDa)

7.62

Isoelectric Point (pI)

36.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
THF_DHG_CYH_C PF02882 32 - 180 2.5e-57 Tetrahydrofolate dehydrogenase/cyclohydrolase, NAD(P)-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 20
AasI GACNNNNNNGTC 1 cut(s) 398
Acc36I ACCTGC 1 cut(s) 20
AccB7I CCANNNNNTGG 1 cut(s) 578
AciI CCGC 2 cut(s) 303, 550
AclWI GGATC 2 cut(s) 64, 77
AcoI YGGCCR 1 cut(s) 168
AfaI GTAC 2 cut(s) 27, 348
AfiI CCNNNNNNNGG 1 cut(s) 578
AjiI CACGTC 1 cut(s) 133
AjnI CCWGG 2 cut(s) 448, 577
AluBI AGCT 6 cut(s) 39, 49, 266, 389, 407, 498
AluI AGCT 6 cut(s) 39, 49, 266, 389, 407, 498
Alw21I GWGCWC 1 cut(s) 206
Alw26I GTCTC 2 cut(s) 316, 521
Alw44I GTGCAC 1 cut(s) 202
AlwI GGATC 2 cut(s) 64, 77
AlwNI CAGNNNCTG 1 cut(s) 578
AoxI GGCC 1 cut(s) 168
ApaLI GTGCAC 1 cut(s) 202
ApeKI GCWGC 2 cut(s) 266, 407
AseI ATTAAT 1 cut(s) 153
AspS9I GGNCC 3 cut(s) 474, 490, 590
AsuHPI GGTGA 1 cut(s) 370
AvaII GGWCC 3 cut(s) 474, 490, 590
BaeGI GKGCMC 1 cut(s) 206
BalI TGGCCA 1 cut(s) 170
BamHI GGATCC 1 cut(s) 69
Bbv12I GWGCWC 1 cut(s) 206
BbvI GCAGC 2 cut(s) 253, 394
BccI CCATC 2 cut(s) 131, 153
BciT130I CCWGG 2 cut(s) 450, 579
BcoDI GTCTC 2 cut(s) 316, 521
BfaI CTAG 1 cut(s) 495
BfmI CTRYAG 1 cut(s) 408
BfuAI ACCTGC 1 cut(s) 20
BisI GCNGC 2 cut(s) 267, 408
BlsI GCNGC 2 cut(s) 268, 409
Bme1390I CCNGG 2 cut(s) 450, 579
Bme18I GGWCC 3 cut(s) 474, 490, 590
BmgBI CACGTC 1 cut(s) 133
BmgT120I GGNCC 3 cut(s) 474, 490, 590
BmiI GGNNCC 4 cut(s) 71, 186, 475, 492
BmrFI CCNGG 2 cut(s) 450, 579
BmrI ACTGGG 1 cut(s) 566
BmsI GCATC 1 cut(s) 314
BmuI ACTGGG 1 cut(s) 566
BpmI CTGGAG 2 cut(s) 471, 600
Bpu10I CCTNAGC 1 cut(s) 233
BsaBI GATNNNNATC 1 cut(s) 68
BsaJI CCNNGG 1 cut(s) 430
BsaXI ACNNNNNCTCC 4 cut(s) 176, 206, 444, 474
Bsc4I CCNNNNNNNGG 1 cut(s) 578
Bse1I ACTGG 2 cut(s) 482, 572
Bse3DI GCAATG 1 cut(s) 308
Bse8I GATNNNNATC 1 cut(s) 68
BseBI CCWGG 2 cut(s) 450, 579
BseDI CCNNGG 1 cut(s) 430
BseGI GGATG 4 cut(s) 97, 115, 142, 145
BseJI GATNNNNATC 1 cut(s) 68
BseLI CCNNNNNNNGG 1 cut(s) 578
BseMI GCAATG 1 cut(s) 308
BseMII CTCAG 2 cut(s) 107, 247
BseNI ACTGG 2 cut(s) 482, 572
BseSI GKGCMC 1 cut(s) 206
BseXI GCAGC 2 cut(s) 253, 394
BshFI GGCC 1 cut(s) 170
BsiHKAI GWGCWC 1 cut(s) 206
BslFI GGGAC 1 cut(s) 487
BslI CCNNNNNNNGG 1 cut(s) 578
BsmAI GTCTC 2 cut(s) 316, 521
BsmBI CGTCTC 1 cut(s) 521
BsmFI GGGAC 1 cut(s) 487
BsnI GGCC 1 cut(s) 170
Bsp1286I GDGCHC 1 cut(s) 206
Bsp1407I TGTACA 1 cut(s) 25
Bsp143I GATC 2 cut(s) 33, 69
BspACI CCGC 2 cut(s) 303, 550
BspANI GGCC 1 cut(s) 170
BspCNI CTCAG 2 cut(s) 108, 246
BspLI GGNNCC 4 cut(s) 71, 186, 475, 492
BspMAI CTGCAG 1 cut(s) 412
BspMI ACCTGC 1 cut(s) 20
BspPI GGATC 2 cut(s) 64, 77
BsrDI GCAATG 1 cut(s) 308
BsrGI TGTACA 1 cut(s) 25
BsrI ACTGG 2 cut(s) 482, 572
BssECI CCNNGG 1 cut(s) 430
BssMI GATC 2 cut(s) 33, 69
Bst2UI CCWGG 2 cut(s) 450, 579
Bst4CI ACNGT 2 cut(s) 78, 337
BstAUI TGTACA 1 cut(s) 25
BstC8I GCNNGC 2 cut(s) 303, 642
BstDEI CTNAG 2 cut(s) 116, 233
BstDSI CCRYGG 1 cut(s) 430
BstF5I GGATG 4 cut(s) 97, 115, 142, 145
BstKTI GATC 2 cut(s) 36, 72
BstMAI GTCTC 2 cut(s) 316, 521
BstMBI GATC 2 cut(s) 33, 69
BstMWI GCNNNNNNNGC 1 cut(s) 641
BstNI CCWGG 2 cut(s) 450, 579
BstNSI RCATGY 1 cut(s) 353
BstSCI CCNGG 2 cut(s) 448, 577
BstSFI CTRYAG 1 cut(s) 408
BstSLI GKGCMC 1 cut(s) 206
BstV1I GCAGC 2 cut(s) 253, 394
BstX2I RGATCY 1 cut(s) 69
BstYI RGATCY 1 cut(s) 69
BsuRI GGCC 1 cut(s) 170
BtgI CCRYGG 1 cut(s) 430
BtrI CACGTC 1 cut(s) 133
BtsCI GGATG 4 cut(s) 97, 115, 142, 145
BtsI GCAGTG 1 cut(s) 552
BtsIMutI CAGTG 2 cut(s) 74, 552
BveI ACCTGC 1 cut(s) 20
Cac8I GCNNGC 2 cut(s) 303, 642
CaiI CAGNNNCTG 1 cut(s) 578
Cfr13I GGNCC 3 cut(s) 474, 490, 590
Csp6I GTAC 2 cut(s) 26, 347
CviAII CATG 4 cut(s) 82, 172, 329, 350
CviQI GTAC 2 cut(s) 26, 347
DdeI CTNAG 2 cut(s) 116, 233
DpnI GATC 2 cut(s) 35, 71
DpnII GATC 2 cut(s) 33, 69
DrdI GACNNNNNNGTC 1 cut(s) 398
DseDI GACNNNNNNGTC 1 cut(s) 398
EaeI YGGCCR 1 cut(s) 168
Eco47I GGWCC 3 cut(s) 474, 490, 590
EcoO109I RGGNCCY 1 cut(s) 490
EcoRII CCWGG 2 cut(s) 448, 577
EcoT22I ATGCAT 1 cut(s) 87
Esp3I CGTCTC 1 cut(s) 521
FaeI CATG 4 cut(s) 85, 175, 332, 353
FalI AAGNNNNNCTT 2 cut(s) 23, 55
FaqI GGGAC 1 cut(s) 487
FatI CATG 4 cut(s) 81, 171, 328, 349
FauI CCCGC 2 cut(s) 310, 543
Fnu4HI GCNGC 2 cut(s) 267, 408
FokI GGATG 4 cut(s) 104, 122, 132, 149
Fsp4HI GCNGC 2 cut(s) 267, 408
FspBI CTAG 1 cut(s) 495
GluI GCNGC 2 cut(s) 267, 408
GsuI CTGGAG 2 cut(s) 471, 600
HaeIII GGCC 1 cut(s) 170
Hin1II CATG 4 cut(s) 85, 175, 332, 353
HindIII AAGCTT 1 cut(s) 47
HinfI GANTC 2 cut(s) 118, 629
HphI GGTGA 1 cut(s) 370
Hpy166II GTNNAC 2 cut(s) 204, 357
Hpy188I TCNGA 1 cut(s) 161
Hpy188III TCNNGA 1 cut(s) 122
Hpy8I GTNNAC 2 cut(s) 204, 357
HpyAV CCTTC 1 cut(s) 174
HpyCH4III ACNGT 2 cut(s) 78, 337
HpyCH4IV ACGT 1 cut(s) 132
HpyCH4V TGCA 6 cut(s) 85, 204, 219, 319, 332, 410
HpyF10VI GCNNNNNNNGC 1 cut(s) 641
HpyF3I CTNAG 2 cut(s) 116, 233
HpySE526I ACGT 1 cut(s) 132
Hsp92II CATG 4 cut(s) 85, 175, 332, 353
Kzo9I GATC 2 cut(s) 33, 69
LmnI GCTCC 2 cut(s) 184, 452
Lsp1109I GCAGC 2 cut(s) 253, 394
LweI GCATC 1 cut(s) 314
MaeI CTAG 1 cut(s) 495
MaeII ACGT 1 cut(s) 132
MaeIII GTNAC 1 cut(s) 400
MalI GATC 2 cut(s) 35, 71
MboI GATC 2 cut(s) 33, 69
MboII GAAGA 3 cut(s) 112, 271, 551
MfeI CAATTG 1 cut(s) 600
MflI RGATCY 1 cut(s) 69
MhlI GDGCHC 1 cut(s) 206
MlsI TGGCCA 1 cut(s) 170
MluCI AATT 3 cut(s) 150, 436, 600
MluNI TGGCCA 1 cut(s) 170
MlyI GAGTC 1 cut(s) 127
MmeI TCCRAC 2 cut(s) 274, 568
MnlI CCTC 5 cut(s) 90, 170, 481, 500, 575
Mox20I TGGCCA 1 cut(s) 170
Mph1103I ATGCAT 1 cut(s) 87
MscI TGGCCA 1 cut(s) 170
MseI TTAA 2 cut(s) 44, 153
MslI CAYNNNNRTG 1 cut(s) 90
Msp20I TGGCCA 1 cut(s) 170
MspA1I CMGCKG 1 cut(s) 407
MspR9I CCNGG 2 cut(s) 450, 579
MunI CAATTG 1 cut(s) 600
MvaI CCWGG 2 cut(s) 450, 579
MwoI GCNNNNNNNGC 1 cut(s) 641
NdeII GATC 2 cut(s) 33, 69
NlaIII CATG 4 cut(s) 85, 175, 332, 353
NlaIV GGNNCC 4 cut(s) 71, 186, 475, 492
NmuCI GTSAC 1 cut(s) 400
NsiI ATGCAT 1 cut(s) 87
NspI RCATGY 1 cut(s) 353
PaqCI CACCTGC 1 cut(s) 20
PfeI GAWTC 1 cut(s) 629
PflMI CCANNNNNTGG 1 cut(s) 578
PfoI TCCNGGA 1 cut(s) 577
PkrI GCNGC 2 cut(s) 268, 409
PleI GAGTC 1 cut(s) 126
PpsI GAGTC 1 cut(s) 126
PpuMI RGGWCCY 1 cut(s) 490
PshBI ATTAAT 1 cut(s) 153
Psp5II RGGWCCY 1 cut(s) 490
Psp6I CCWGG 2 cut(s) 448, 577
PspGI CCWGG 2 cut(s) 448, 577
PspN4I GGNNCC 4 cut(s) 71, 186, 475, 492
PspPI GGNCC 3 cut(s) 474, 490, 590
PspPPI RGGWCCY 1 cut(s) 490
PstI CTGCAG 1 cut(s) 412
PstNI CAGNNNCTG 1 cut(s) 578
PsuI RGATCY 1 cut(s) 69
PvuII CAGCTG 1 cut(s) 407
RsaI GTAC 2 cut(s) 27, 348
RsaNI GTAC 2 cut(s) 26, 347
RseI CAYNNNNRTG 1 cut(s) 90
SaqAI TTAA 2 cut(s) 44, 153
SatI GCNGC 2 cut(s) 267, 408
Sau3AI GATC 2 cut(s) 33, 69
Sau96I GGNCC 3 cut(s) 474, 490, 590
SchI GAGTC 1 cut(s) 127
ScrFI CCNGG 2 cut(s) 450, 579
SduI GDGCHC 1 cut(s) 206
SfaNI GCATC 1 cut(s) 314
SfcI CTRYAG 1 cut(s) 408
SinI GGWCC 3 cut(s) 474, 490, 590
SmiMI CAYNNNNRTG 1 cut(s) 90
Sse9I AATT 3 cut(s) 150, 436, 600
SsiI CCGC 2 cut(s) 303, 550
SspI AATATT 1 cut(s) 289
SspMI CTAG 1 cut(s) 495
StyD4I CCNGG 2 cut(s) 448, 577
TaaI ACNGT 2 cut(s) 78, 337
TaiI ACGT 1 cut(s) 135
TasI AATT 3 cut(s) 150, 436, 600
TatI WGTACW 1 cut(s) 25
TfiI GAWTC 1 cut(s) 629
Tru1I TTAA 2 cut(s) 44, 153
Tru9I TTAA 2 cut(s) 44, 153
TscAI CASTG 2 cut(s) 81, 552
TseFI GTSAC 1 cut(s) 400
TseI GCWGC 2 cut(s) 266, 407
Tsp45I GTSAC 1 cut(s) 400
TspDTI ATGAA 2 cut(s) 70, 552
TspGWI ACGGA 2 cut(s) 189, 419
TspRI CASTG 2 cut(s) 81, 552
Van91I CCANNNNNTGG 1 cut(s) 578
VneI GTGCAC 1 cut(s) 202
VpaK11BI GGWCC 3 cut(s) 474, 490, 590
VspI ATTAAT 1 cut(s) 153
XceI RCATGY 1 cut(s) 353
XspI CTAG 1 cut(s) 495
Zsp2I ATGCAT 1 cut(s) 87
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.