Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000006

Orthogroup ID

OG0006558

Physical Location & Seq

Genomic Coordinates Reverse (-)

41589998 .. 41593441

Gene Structure 3444 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_6G00420300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1215 bp

ATGGGGCTGGTTTCTACAGGAAGTAAATTCTGGAATGGTGAAGCGCAAAGCGAGGAGGCTCAGAATAATACAAATTACAAGTTTAGGGGGGCAGAGAGAAAGGGAGAGCGCAAAGCCACAATGATGCCAAGAGTGGCCATGTCATGGCGCAATGGACTCAGAATGAGAATCCCATGGAGAACCAGAGCCCTCAACACCTTAAACGACGACGTTCACTCCTCCCGCCCAATTATCGTGTCTCCTCCTCTTGTCTCTCTAGACATTCCTGACGATTGGACTCCTTCTCCACCCTCTTCACATTTTGATTTTCCTATCAATTGCTCCCATGAGCAGACTGCTGCTGTTATTGATGGGAAGTCTATTGCGGACGATATTAGGTCGAGAATAGGGAGTGAGGTAAGGAGGATGAAAAAGAGCATTGGAATGGTGCCTGGGTTGGGTGTAATTATGGTTGGTCAGAGAAAGGACTCTGAGACTTATGTCAGGAGCAAGATCATGGCTTGTGAAGAAGTTGGAATTAAATCAAATGTGGCTCACCTGCCTGAGCATTGTACAAAAGATCAGCTTCTTAAAGCTTTGTCAAAGTTTGATGTGGATCCCTCTGTTCATGGTATTCTCGTGCAACTTCCTCTACCACAACATTTGGATGAGAGAAAAATTATGGATGTGCTGAGTCCGGAGAAAGATGTGGATGGCTTCCATCCAATTAATATGGGAAATCTTGCCATGAGAGGAAGGGAGCCTATGTTTATTCCATGCACTCCAAAGGGTTGCATAGAGTTGTTGCTCAGGTCTGGCGTGGAAATAGTTGGGAAGAAAGCTGCTGTCATTGGCAGAAGTAATATTGTAGGATTGCCCGCATCGTTGCTTTTGCAGAGGCACCATGCAACAGTCAGCATTGTACATGCATTCACAAAGAACCCAGAACATATCACCTGTGAAGCTGACATTGTGGTCACCGCTGCAGGAGTGCCTAATCTTGTCCGTGGAAATTGGCTAAAACCTGGAGCGGTTGTCATCGACGTTGGGACCAATCCAGTTGAGGACCCTAGCTGTGAGCGAGGTTATCGCCTCATAGGAGATGTTTGCTATGAAGAAGCAGTGCGGGTGGTGTCAGCCATCACCCCAGTCCCTGGAGGAGTTGGACCCATGACAATTGCTATGCTTCTATCAAATACTCTTGATTCAGCAAAGCGAGCCTACGCCTTTACTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004477 GO:0004488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006730 GO:0008150 GO:0008152 GO:0009987 GO:0016491 GO:0016645 GO:0016646 GO:0016787 GO:0016810 GO:0016814 GO:0019238 GO:0044237 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0055114

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

THF_DHG_CYH THF_DHG_CYH_C

Protein Analysis

404

Amino Acids

44.21

Weight (kDa)

8.56

Isoelectric Point (pI)

38.73

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
THF_DHG_CYH	PF00763	116 - 231	2.8e-39	Tetrahydrofolate dehydrogenase/cyclohydrolase, catalytic domain
THF_DHG_CYH_C	PF02882	252 - 400	2.1e-56	Tetrahydrofolate dehydrogenase/cyclohydrolase, NAD(P)-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006558)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G38660 AT2G38660 AT2G38660 AT2G38660 AT2G38660 AT2G38660 AT2G38660 AT2G38660
fragaria_vesca	FvH4_6g09070 FvH4_6g09070 FvH4_6g09070 FvH4_6g09070 FvH4_6g09070
malus_domestica	MD04G1181400.v1.1
prunus_persica	Prupe.6G300800_v2.0.a1
rosa_chinensis	RchiOBHm_Chr3g0459301
rosa_laevigata	RLG00000025095
rosa_multiflora	Rmu_co8497659.1_g000001 Rmu_sc0032631.1_g000001
rosa_roxburghii	Rroxscaffold_6G00420300
rosa_rugosa	Rorug03G0035100
rosa_samantha	Rh3AG093000 Rh3BG096100 Rh3CG097100 Rh3DG096700
rosa_wichuraiana	Rw3G008000

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	546
AasI	GACNNNNNNGTC	1 cut(s)	953
Acc36I	ACCTGC	1 cut(s)	546
AccB1I	GGYRCC	2 cut(s)	427, 879
AccB7I	CCANNNNNTGG	2 cut(s)	144, 1133
AccBSI	CCGCTC	1 cut(s)	1010
AccIII	TCCGGA	1 cut(s)	676
AciI	CCGC	6 cut(s)	223, 365, 858, 960, 1010, 1105
AclWI	GGATC	2 cut(s)	590, 603
AcoI	YGGCCR	1 cut(s)	135
AcsI	RAATTY	1 cut(s)	26
AfaI	GTAC	2 cut(s)	553, 903
AfiI	CCNNNNNNNGG	3 cut(s)	144, 437, 1133
AjnI	CCWGG	3 cut(s)	430, 1003, 1132
AluBI	AGCT	5 cut(s)	565, 575, 821, 944, 1053
AluI	AGCT	5 cut(s)	565, 575, 821, 944, 1053
Alw26I	GTCTC	3 cut(s)	243, 256, 467
AlwI	GGATC	2 cut(s)	590, 603
AlwNI	CAGNNNCTG	1 cut(s)	1133
Aor13HI	TCCGGA	1 cut(s)	676
AoxI	GGCC	1 cut(s)	135
ApeKI	GCWGC	3 cut(s)	338, 821, 962
ApoI	RAATTY	1 cut(s)	26
AseI	ATTAAT	1 cut(s)	708
AspLEI	GCGC	3 cut(s)	46, 111, 150
AspS9I	GGNCC	3 cut(s)	1029, 1045, 1145
AsuHPI	GGTGA	5 cut(s)	50, 527, 925, 949, 1114
AvaII	GGWCC	3 cut(s)	1029, 1045, 1145
BalI	TGGCCA	1 cut(s)	137
BamHI	GGATCC	1 cut(s)	595
BanI	GGYRCC	2 cut(s)	427, 879
BanII	GRGCYC	1 cut(s)	190
BauI	CACGAG	1 cut(s)	617
BbvI	GCAGC	3 cut(s)	325, 808, 949
BccI	CCATC	4 cut(s)	344, 686, 708, 1127
BciT130I	CCWGG	3 cut(s)	432, 1005, 1134
BcoDI	GTCTC	3 cut(s)	243, 256, 467
BfaI	CTAG	2 cut(s)	257, 1050
BfmI	CTRYAG	2 cut(s)	15, 963
BfuAI	ACCTGC	1 cut(s)	546
BisI	GCNGC	3 cut(s)	339, 822, 963
BlsI	GCNGC	3 cut(s)	340, 823, 964
Bme1390I	CCNGG	3 cut(s)	432, 1005, 1134
Bme18I	GGWCC	3 cut(s)	1029, 1045, 1145
BmgT120I	GGNCC	3 cut(s)	1029, 1045, 1145
BmiI	GGNNCC	7 cut(s)	429, 597, 741, 881, 1030, 1047, 1147
BmrFI	CCNGG	3 cut(s)	432, 1005, 1134
BmrI	ACTGGG	1 cut(s)	1121
BmsI	GCATC	2 cut(s)	114, 869
BmuI	ACTGGG	1 cut(s)	1121
BoxI	GACNNNNGTC	1 cut(s)	479
BpmI	CTGGAG	2 cut(s)	1026, 1155
Bpu10I	CCTNAGC	2 cut(s)	543, 788
BsaBI	GATNNNNATC	1 cut(s)	594
BsaJI	CCNNGG	4 cut(s)	173, 431, 985, 1132
BsaWI	WCCGGW	1 cut(s)	676
BsaXI	ACNNNNNCTCC	8 cut(s)	200, 230, 268, 298, 731, 761, 999, 1029
Bsc4I	CCNNNNNNNGG	3 cut(s)	144, 437, 1133
Bse1I	ACTGG	2 cut(s)	1037, 1127
Bse3DI	GCAATG	1 cut(s)	157
Bse8I	GATNNNNATC	1 cut(s)	594
BseAI	TCCGGA	1 cut(s)	676
BseBI	CCWGG	3 cut(s)	432, 1005, 1134
BseDI	CCNNGG	4 cut(s)	173, 431, 985, 1132
BseGI	GGATG	5 cut(s)	411, 652, 670, 697, 700
BseJI	GATNNNNATC	1 cut(s)	594
BseLI	CCNNNNNNNGG	3 cut(s)	144, 437, 1133
BseMI	GCAATG	1 cut(s)	157
BseMII	CTCAG	6 cut(s)	74, 172, 462, 534, 662, 802
BseNI	ACTGG	2 cut(s)	1037, 1127
BseRI	GAGGAG	5 cut(s)	68, 208, 231, 234, 1152
BseXI	GCAGC	3 cut(s)	325, 808, 949
BshFI	GGCC	1 cut(s)	137
BshNI	GGYRCC	2 cut(s)	427, 879
BsiSI	CCGG	1 cut(s)	677
BslFI	GGGAC	2 cut(s)	1042, 1115
BslI	CCNNNNNNNGG	3 cut(s)	144, 437, 1133
BsmAI	GTCTC	3 cut(s)	243, 256, 467
BsmFI	GGGAC	2 cut(s)	1042, 1115
BsmI	GAATGC	1 cut(s)	908
BsnI	GGCC	1 cut(s)	137
Bsp1286I	GDGCHC	1 cut(s)	190
Bsp13I	TCCGGA	1 cut(s)	676
Bsp1407I	TGTACA	2 cut(s)	551, 901
Bsp143I	GATC	3 cut(s)	492, 559, 595
Bsp19I	CCATGG	1 cut(s)	173
BspACI	CCGC	6 cut(s)	223, 365, 858, 960, 1010, 1105
BspANI	GGCC	1 cut(s)	137
BspCNI	CTCAG	6 cut(s)	73, 171, 463, 535, 663, 801
BspEI	TCCGGA	1 cut(s)	676
BspLI	GGNNCC	7 cut(s)	429, 597, 741, 881, 1030, 1047, 1147
BspMAI	CTGCAG	1 cut(s)	967
BspMI	ACCTGC	1 cut(s)	546
BspPI	GGATC	2 cut(s)	590, 603
BspT107I	GGYRCC	2 cut(s)	427, 879
BsrBI	CCGCTC	1 cut(s)	1010
BsrDI	GCAATG	1 cut(s)	157
BsrGI	TGTACA	2 cut(s)	551, 901
BsrI	ACTGG	2 cut(s)	1037, 1127
BssECI	CCNNGG	4 cut(s)	173, 431, 985, 1132
BssMI	GATC	3 cut(s)	492, 559, 595
BssSI	CACGAG	1 cut(s)	617
BssT1I	CCWWGG	1 cut(s)	173
Bst2BI	CACGAG	1 cut(s)	617
Bst2UI	CCWGG	3 cut(s)	432, 1005, 1134
Bst4CI	ACNGT	1 cut(s)	892
Bst6I	CTCTTC	1 cut(s)	298
BstAUI	TGTACA	2 cut(s)	551, 901
BstC8I	GCNNGC	2 cut(s)	858, 1197
BstDEI	CTNAG	6 cut(s)	60, 158, 471, 543, 671, 788
BstDSI	CCRYGG	2 cut(s)	173, 985
BstEII	GGTNACC	1 cut(s)	955
BstF5I	GGATG	5 cut(s)	411, 652, 670, 697, 700
BstHHI	GCGC	3 cut(s)	46, 111, 150
BstKTI	GATC	3 cut(s)	495, 562, 598
BstMAI	GTCTC	3 cut(s)	243, 256, 467
BstMBI	GATC	3 cut(s)	492, 559, 595
BstMWI	GCNNNNNNNGC	1 cut(s)	1196
BstNI	CCWGG	3 cut(s)	432, 1005, 1134
BstNSI	RCATGY	1 cut(s)	908
BstPAI	GACNNNNGTC	1 cut(s)	479
BstPI	GGTNACC	1 cut(s)	955
BstSCI	CCNGG	3 cut(s)	430, 1003, 1132
BstSFI	CTRYAG	2 cut(s)	15, 963
BstV1I	GCAGC	3 cut(s)	325, 808, 949
BstX2I	RGATCY	1 cut(s)	595
BstYI	RGATCY	1 cut(s)	595
BsuRI	GGCC	1 cut(s)	137
BtgI	CCRYGG	2 cut(s)	173, 985
BtsCI	GGATG	5 cut(s)	411, 652, 670, 697, 700
BtsI	GCAGTG	1 cut(s)	1107
BtsIMutI	CAGTG	1 cut(s)	1107
BveI	ACCTGC	1 cut(s)	546
Cac8I	GCNNGC	2 cut(s)	858, 1197
CaiI	CAGNNNCTG	1 cut(s)	1133
CfoI	GCGC	3 cut(s)	46, 111, 150
Cfr13I	GGNCC	3 cut(s)	1029, 1045, 1145
Csp6I	GTAC	2 cut(s)	552, 902
CspCI	CAANNNNNGTGG	2 cut(s)	624, 659
CviQI	GTAC	2 cut(s)	552, 902
DdeI	CTNAG	6 cut(s)	60, 158, 471, 543, 671, 788
DpnI	GATC	3 cut(s)	494, 561, 597
DpnII	GATC	3 cut(s)	492, 559, 595
DrdI	GACNNNNNNGTC	1 cut(s)	953
DseDI	GACNNNNNNGTC	1 cut(s)	953
EaeI	YGGCCR	1 cut(s)	135
Eam1104I	CTCTTC	1 cut(s)	298
EarI	CTCTTC	1 cut(s)	298
Eco130I	CCWWGG	1 cut(s)	173
Eco24I	GRGCYC	1 cut(s)	190
Eco47I	GGWCC	3 cut(s)	1029, 1045, 1145
Eco91I	GGTNACC	1 cut(s)	955
EcoO109I	RGGNCCY	1 cut(s)	1045
EcoO65I	GGTNACC	1 cut(s)	955
EcoRII	CCWGG	3 cut(s)	430, 1003, 1132
EcoT14I	CCWWGG	1 cut(s)	173
EcoT22I	ATGCAT	1 cut(s)	910
EcoT38I	GRGCYC	1 cut(s)	190
ErhI	CCWWGG	1 cut(s)	173
FalI	AAGNNNNNCTT	2 cut(s)	549, 581
FaqI	GGGAC	2 cut(s)	1042, 1115
FauI	CCCGC	3 cut(s)	230, 865, 1098
Fnu4HI	GCNGC	3 cut(s)	339, 822, 963
FokI	GGATG	5 cut(s)	418, 659, 677, 687, 704
FriOI	GRGCYC	1 cut(s)	190
Fsp4HI	GCNGC	3 cut(s)	339, 822, 963
FspBI	CTAG	2 cut(s)	257, 1050
GlaI	GCGC	3 cut(s)	45, 110, 149
GluI	GCNGC	3 cut(s)	339, 822, 963
GsuI	CTGGAG	2 cut(s)	1026, 1155
HaeIII	GGCC	1 cut(s)	137
HapII	CCGG	1 cut(s)	677
HhaI	GCGC	3 cut(s)	46, 111, 150
Hin6I	GCGC	3 cut(s)	44, 109, 148
HinP1I	GCGC	3 cut(s)	44, 109, 148
HindIII	AAGCTT	1 cut(s)	573
HinfI	GANTC	6 cut(s)	156, 168, 277, 467, 673, 1184
HpaII	CCGG	1 cut(s)	677
HphI	GGTGA	5 cut(s)	50, 527, 925, 949, 1114
Hpy166II	GTNNAC	1 cut(s)	214
Hpy188I	TCNGA	4 cut(s)	63, 161, 459, 472
Hpy188III	TCNNGA	7 cut(s)	31, 257, 266, 381, 484, 677, 1181
Hpy8I	GTNNAC	1 cut(s)	214
Hpy99I	CGWCG	3 cut(s)	209, 212, 1025
HpyAV	CCTTC	2 cut(s)	291, 729
HpyCH4III	ACNGT	1 cut(s)	892
HpyCH4IV	ACGT	2 cut(s)	210, 1023
HpyCH4V	TGCA	7 cut(s)	622, 759, 774, 874, 887, 908, 965
HpyF10VI	GCNNNNNNNGC	1 cut(s)	1196
HpyF3I	CTNAG	6 cut(s)	60, 158, 471, 543, 671, 788
HpySE526I	ACGT	2 cut(s)	210, 1023
HspAI	GCGC	3 cut(s)	44, 109, 148
Kpn2I	TCCGGA	1 cut(s)	676
Kzo9I	GATC	3 cut(s)	492, 559, 595
LmnI	GCTCC	4 cut(s)	326, 486, 739, 1007
Lsp1109I	GCAGC	3 cut(s)	325, 808, 949
LweI	GCATC	2 cut(s)	114, 869
MaeI	CTAG	2 cut(s)	257, 1050
MaeII	ACGT	2 cut(s)	210, 1023
MaeIII	GTNAC	1 cut(s)	955
MalI	GATC	3 cut(s)	494, 561, 597
MbiI	CCGCTC	1 cut(s)	1010
MboI	GATC	3 cut(s)	492, 559, 595
MboII	GAAGA	4 cut(s)	285, 518, 826, 1106
MfeI	CAATTG	2 cut(s)	316, 1155
MflI	RGATCY	1 cut(s)	595
MhlI	GDGCHC	1 cut(s)	190
MlsI	TGGCCA	1 cut(s)	137
MluNI	TGGCCA	1 cut(s)	137
MlyI	GAGTC	4 cut(s)	150, 271, 461, 682
MmeI	TCCRAC	2 cut(s)	493, 1123
Mox20I	TGGCCA	1 cut(s)	137
Mph1103I	ATGCAT	1 cut(s)	910
MroI	TCCGGA	1 cut(s)	676
MscI	TGGCCA	1 cut(s)	137
MseI	TTAA	4 cut(s)	200, 519, 570, 708
MslI	CAYNNNNRTG	3 cut(s)	122, 422, 645
Msp20I	TGGCCA	1 cut(s)	137
MspA1I	CMGCKG	1 cut(s)	962
MspI	CCGG	1 cut(s)	677
MspR9I	CCNGG	3 cut(s)	432, 1005, 1134
MunI	CAATTG	2 cut(s)	316, 1155
Mva1269I	GAATGC	1 cut(s)	908
MvaI	CCWGG	3 cut(s)	432, 1005, 1134
MwoI	GCNNNNNNNGC	1 cut(s)	1196
NcoI	CCATGG	1 cut(s)	173
NdeII	GATC	3 cut(s)	492, 559, 595
NlaIV	GGNNCC	7 cut(s)	429, 597, 741, 881, 1030, 1047, 1147
NmuCI	GTSAC	1 cut(s)	955
NsiI	ATGCAT	1 cut(s)	910
NspI	RCATGY	1 cut(s)	908
PaqCI	CACCTGC	1 cut(s)	546
PctI	GAATGC	1 cut(s)	908
PfeI	GAWTC	2 cut(s)	168, 1184
PflMI	CCANNNNNTGG	2 cut(s)	144, 1133
PkrI	GCNGC	3 cut(s)	340, 823, 964
PleI	GAGTC	4 cut(s)	150, 271, 461, 681
PpsI	GAGTC	4 cut(s)	150, 271, 461, 681
PpuMI	RGGWCCY	1 cut(s)	1045
PshAI	GACNNNNGTC	1 cut(s)	479
PshBI	ATTAAT	1 cut(s)	708
Psp5II	RGGWCCY	1 cut(s)	1045
Psp6I	CCWGG	3 cut(s)	430, 1003, 1132
PspEI	GGTNACC	1 cut(s)	955
PspGI	CCWGG	3 cut(s)	430, 1003, 1132
PspN4I	GGNNCC	7 cut(s)	429, 597, 741, 881, 1030, 1047, 1147
PspPI	GGNCC	3 cut(s)	1029, 1045, 1145
PspPPI	RGGWCCY	1 cut(s)	1045
PstI	CTGCAG	1 cut(s)	967
PstNI	CAGNNNCTG	1 cut(s)	1133
PsuI	RGATCY	1 cut(s)	595
RsaI	GTAC	2 cut(s)	553, 903
RsaNI	GTAC	2 cut(s)	552, 902
RseI	CAYNNNNRTG	3 cut(s)	122, 422, 645
SaqAI	TTAA	4 cut(s)	200, 519, 570, 708
SatI	GCNGC	3 cut(s)	339, 822, 963
Sau3AI	GATC	3 cut(s)	492, 559, 595
Sau96I	GGNCC	3 cut(s)	1029, 1045, 1145
SchI	GAGTC	4 cut(s)	150, 271, 461, 682
ScrFI	CCNGG	3 cut(s)	432, 1005, 1134
SduI	GDGCHC	1 cut(s)	190
SfaNI	GCATC	2 cut(s)	114, 869
SfcI	CTRYAG	2 cut(s)	15, 963
SinI	GGWCC	3 cut(s)	1029, 1045, 1145
SmiMI	CAYNNNNRTG	3 cut(s)	122, 422, 645
SsiI	CCGC	6 cut(s)	223, 365, 858, 960, 1010, 1105
SspI	AATATT	1 cut(s)	844
SspMI	CTAG	2 cut(s)	257, 1050
StyD4I	CCNGG	3 cut(s)	430, 1003, 1132
StyI	CCWWGG	1 cut(s)	173
TaaI	ACNGT	1 cut(s)	892
TaiI	ACGT	2 cut(s)	213, 1026
TaqI	TCGA	2 cut(s)	380, 1020
TatI	WGTACW	2 cut(s)	551, 901
TfiI	GAWTC	2 cut(s)	168, 1184
Tru1I	TTAA	4 cut(s)	200, 519, 570, 708
Tru9I	TTAA	4 cut(s)	200, 519, 570, 708
TscAI	CASTG	1 cut(s)	1107
TseFI	GTSAC	1 cut(s)	955
TseI	GCWGC	3 cut(s)	338, 821, 962
Tsp45I	GTSAC	1 cut(s)	955
TspDTI	ATGAA	3 cut(s)	422, 596, 1107
TspGWI	ACGGA	1 cut(s)	974
TspRI	CASTG	1 cut(s)	1107
Van91I	CCANNNNNTGG	2 cut(s)	144, 1133
VpaK11BI	GGWCC	3 cut(s)	1029, 1045, 1145
VspI	ATTAAT	1 cut(s)	708
XapI	RAATTY	1 cut(s)	26
XbaI	TCTAGA	1 cut(s)	256
XceI	RCATGY	1 cut(s)	908
XspI	CTAG	2 cut(s)	257, 1050
Zsp2I	ATGCAT	1 cut(s)	910

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.