FvH4_6g09910

B3 domain-containing

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0001663
Physical Location & Seq
Forward (+)
5883382 .. 5889262
5881 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g09910.t8

Sequence Viewer

Length: 2475 bp
ATGGATCCGAGTGCTTGTATGAACGCCTACTGCGGCAGCTCGAGCTCGATCGAATGGAAGAAAGGCTGGGCTTTGCGATCTGGTAGATTCGCCAATCTATGTCACAAGTGCGGGTCGGCATACGAGCAATCTGTTTTTTGTGATGTTTTCCATTCAAAAGAATCCGGTTGGAGGGAGTGTGCTCAATGTGGAAAGCGTCTTCATTGTGGGTGCATTGCTTCTAGGAGTCTGCTTGATTTCCTTGATGGTGGTGGTGTCAAGTGTACACACTGCACGAAAAATTCGGAACCTCACCCTATTGCAAGTGATGAAAAGCCTGATGGACCCGGCACATCCAAAATCAGTGAGCTGAAATCAACTCCTTCAGACAATCACTTGGATAGAAGCAATGTTGATAATGTGAAGCTTATCCAGTTGGAAAATGATAAAGAGTGTAATGGGCTCAGGAACTTGCTTCAATCTCAGAACAATGAAACAGTTGGGTTATTGCAGAAAATGAAGCAGGATGATGTTCCAGCACCTGTGGTTGAGATTGGAGGCACAGGATATGCAAATCCTTTTCCTGGTATCGTTGTTGACGAACACACTAGGACATCCTCCCTGTTCTTACAGGGAGCTAGGTCTCGTCATCTTTTACCCAAGCCCCCAAAGTTGGCCCTTGCCACAGGTTTGGAGGAAAATTCTACAATGGCTTCACAGTCACGTGTTGCACGGCCACCTGCTGAAGGACGGGGGAGGAATCAGTTACTTCCCCGATACTGGCCCAGGATCACGGACCAAGAGTTACAGCAAATATCTGGAGATCCAAATTCCACAATTGTACCGTTGTTTGAAAAGATGCTGAGTGCCAGTGATGCTGGTCGTATTGGTCGTTTGGTGCTCCCTAAAGCATGTGCTGAAGCGTATTTTCCTCCTATCTCTCAACCCGAGGGCCTTCCTTTGAGGATTCAAGATGTTAAGGGAAAGGAATGGGTGTTTCAGTTCAGATTCTGGCCTAACAATAACAGTAGGATGTACGTGTTAGAGGGTGTAACCCCTTGCATACAGTCGATGCAGTTGCAAGCTGGAGATACTGTGACATTCAGTCGTATGGACCCTGAAGGAAAGCTTATTATGGGGTTTCGAAAGGCATCTAATTCAGCTTCAATGCAGTCAACGAAGGGCATGGATCCGCATCTAAGTGCACTGTCCAAGCAATTGACTACAGCTCATGGTGATCTGAGCTGGCATAAATCTGAAAATCCTGAAAGCAGGGCAAGGGAAGGCTTGCTGCTGCAATCATTAGTGGTTCCGGAGAGAAAAAGAACTAGGAATATTGGGTCTAAGAGTAAAAGGTTGCTCATTGATAGCCAAGATGTTCTAGAGGTGAAGCTTACTTGGGAAGAGGCTCAGGATTTACTTCGTCCTCCTCCAGCTGTTAACCCAAGCACTGTCATGATCGAGGATCTTGAATTCGAAGAATATGAAGAACCTCCTGTGTTTGGCAAAAGGAGTATTTTCATAGTCCGGTCAACTGGGGAACATGAGCAATGGGCTCAGTGTGATGGTTGCTCTAAATGGCGAAGGTTGCCAGTTGATGTGCTACTTCCATCTAAGTGGATGTGTACAGACAATGTTTGGGATCAAAACAGGTGTTCGTGCTCTGCACCAGATGAATTAACCCCTAAGGAACTGGAAAGTTTTCTCAGACTGAGCAAGGAATTCAAGAAAAGGAGAATGGCAACAAACCATAATCCAACCCAGGAACATGAATCTTCTGGGCTTGATGCTTTGGCCAATGCTGCAATACTCGGAGACAATGTGGCTGACCCAGGAACTGCATCAGTTGCTACAACAACAAAACATCCCAGGCATCGTCCTGGCTGCTCATGCATTGTGTGCATCCAGCCCCCAAGTGGGAAGGGCAAGCATAAGCCTTCATGCACGTGCAATGTGTGCATGACAGTTAAACGACGTTTTAAAACCCTAATGATAAACAAGAAGAAACGTCAATCAGAACGTGAAGCAGAGATTGCCGGTAGAAACCAGCTTGCATGGGGTCCTAGGGATGATGCAGAAGTAGACAGCACTTCTAGGCATCTATCATCACACCTTGATCCTTCAGACAACGAAGCTAAGTCTCCAAATGAATTAGAATCTAAGAGCCAACTTAAAATGGCCGAAAGTGGGAAGGGAAAGTTAGACTTGAATTGCCATCCTGGCCGAGAAGTAGACTTGCCAGCGGAGCCAAGTCAGTTGAGCATGATGTCCCTTCTTCAAGTAGCAACGCTTCCTCTAGATAGTTATTTGAAACAAACCGGTCTTACCAGCTTGGTAACTGAACAGCAGACAAGCTCATCACCTCCTGTGCCACCACAAGCGACCGAGGAGAATGAGGAACAGCTTAATGGGGATCAATGTCTTGTTTCTATTGTTCAAGATCAGGAAAGTGGAGGTGAAGAAAGGCAGGATCAAAGCCAAGAGGATCCTCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3 zf-CW

Protein Analysis

825

Amino Acids

91.29

Weight (kDa)

6.33

Isoelectric Point (pI)

62.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 159 - 258 2.8e-15 B3 DNA binding domain
zf-CW PF07496 428 - 470 1.3e-10 CW-type Zinc Finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 727
Acc36I ACCTGC 1 cut(s) 727
AccI GTMKAC 2 cut(s) 2061, 2211
AccIII TCCGGA 1 cut(s) 1291
AciI CCGC 4 cut(s) 33, 111, 1172, 2222
AcoI YGGCCR 4 cut(s) 713, 1773, 2157, 2200
AcsI RAATTY 5 cut(s) 280, 679, 808, 1451, 1700
AcuI CTGAAG 5 cut(s) 348, 744, 918, 1119, 2085
AcvI CACGTG 2 cut(s) 704, 1926
AfaI GTAC 4 cut(s) 265, 822, 1016, 1606
AfiI CCNNNNNNNGG 9 cut(s) 171, 563, 652, 725, 759, 1481, 1895, 1896, 2166
AflIII ACRYGT 2 cut(s) 703, 1017
AgeI ACCGGT 1 cut(s) 2297
AhdI GACNNNNNGTC 1 cut(s) 1083
AjnI CCWGG 7 cut(s) 562, 764, 1740, 1810, 1847, 1858, 2197
Alw21I GWGCWC 5 cut(s) 47, 184, 882, 1186, 1643
Alw26I GTCTC 3 cut(s) 627, 1788, 2124
Alw44I GTGCAC 1 cut(s) 1182
AlwNI CAGNNNCTG 3 cut(s) 521, 990, 1817
Ama87I CYCGRG 2 cut(s) 40, 926
Aor13HI TCCGGA 1 cut(s) 1291
AoxI GGCC 8 cut(s) 654, 713, 761, 931, 992, 1773, 2157, 2200
ApaLI GTGCAC 1 cut(s) 1182
ApeKI GCWGC 5 cut(s) 36, 1270, 1273, 1781, 1863
ApoI RAATTY 5 cut(s) 280, 679, 808, 1451, 1700
AsiGI ACCGGT 1 cut(s) 2297
Asp700I GAANNNNTTC 1 cut(s) 1680
AspA2I CCTAGG 1 cut(s) 2042
AspS9I GGNCC 7 cut(s) 323, 655, 762, 775, 931, 1093, 2039
AsuC2I CCSGG 1 cut(s) 327
AsuHPI GGTGA 5 cut(s) 284, 1226, 1378, 2331, 2447
AsuII TTCGAA 2 cut(s) 1123, 1455
AvaI CYCGRG 2 cut(s) 40, 926
AvaII GGWCC 4 cut(s) 323, 775, 1093, 2039
AvrII CCTAGG 1 cut(s) 2042
AxyI CCTNAGG 1 cut(s) 1665
BaeGI GKGCMC 1 cut(s) 1186
BaeI ACNNNNGTAYC 2 cut(s) 804, 837
BalI TGGCCA 1 cut(s) 1775
BamHI GGATCC 3 cut(s) 4, 1168, 2464
BanII GRGCYC 3 cut(s) 47, 444, 1537
BarI GAAGNNNNNNTAC 2 cut(s) 2253, 2285
BbrPI CACGTG 2 cut(s) 704, 1926
BbsI GAAGAC 1 cut(s) 191
Bbv12I GWGCWC 5 cut(s) 47, 184, 882, 1186, 1643
BbvI GCAGC 5 cut(s) 48, 1257, 1260, 1768, 1850
BccI CCATC 5 cut(s) 239, 314, 1538, 1597, 2202
BceAI ACGGC 1 cut(s) 728
BcgI CGANNNNNNTGC 2 cut(s) 1039, 1073
BciT130I CCWGG 7 cut(s) 564, 766, 1742, 1812, 1849, 1860, 2199
BcnI CCSGG 1 cut(s) 327
BcoDI GTCTC 3 cut(s) 627, 1788, 2124
BfaI CTAG 8 cut(s) 222, 588, 618, 1308, 1361, 2043, 2073, 2276
BfmI CTRYAG 1 cut(s) 1203
BfuAI ACCTGC 1 cut(s) 727
BglI GCCNNNNNGGC 1 cut(s) 2199
BisI GCNGC 6 cut(s) 34, 37, 1271, 1274, 1782, 1864
BlnI CCTAGG 1 cut(s) 2042
BlsI GCNGC 6 cut(s) 35, 38, 1272, 1275, 1783, 1865
Bme1390I CCNGG 8 cut(s) 327, 564, 766, 1742, 1812, 1849, 1860, 2199
Bme18I GGWCC 4 cut(s) 323, 775, 1093, 2039
BmeRI GACNNNNNGTC 1 cut(s) 1083
BmeT110I CYCGRG 2 cut(s) 40, 926
BmgT120I GGNCC 7 cut(s) 323, 655, 762, 775, 931, 1093, 2039
BmiI GGNNCC 9 cut(s) 6, 288, 325, 1095, 1170, 1290, 2040, 2226, 2466
BmrFI CCNGG 8 cut(s) 327, 564, 766, 1742, 1812, 1849, 1860, 2199
BmrI ACTGGG 1 cut(s) 1524
BmuI ACTGGG 1 cut(s) 1524
BpiI GAAGAC 1 cut(s) 191
BpmI CTGGAG 3 cut(s) 819, 1086, 1395
Bpu10I CCTNAGC 2 cut(s) 443, 1389
Bpu14I TTCGAA 2 cut(s) 1123, 1455
BpuMI CCSGG 1 cut(s) 327
BsaAI YACGTR 3 cut(s) 704, 1018, 1926
BsaBI GATNNNNATC 1 cut(s) 1173
BsaI GGTCTC 1 cut(s) 627
BsaJI CCNNGG 7 cut(s) 764, 927, 1740, 1810, 1847, 2042, 2364
BsaWI WCCGGW 4 cut(s) 164, 1291, 1506, 2297
BsaXI ACNNNNNCTCC 6 cut(s) 163, 193, 1059, 1089, 1785, 1815
Bsc4I CCNNNNNNNGG 9 cut(s) 171, 563, 652, 725, 759, 1481, 1895, 1896, 2166
Bse118I RCCGGY 2 cut(s) 2015, 2297
Bse1I ACTGG 6 cut(s) 412, 764, 849, 1519, 1571, 1677
Bse21I CCTNAGG 1 cut(s) 1665
Bse3DI GCAATG 4 cut(s) 213, 394, 1535, 1936
Bse8I GATNNNNATC 1 cut(s) 1173
BseAI TCCGGA 1 cut(s) 1291
BseBI CCWGG 7 cut(s) 564, 766, 1742, 1812, 1849, 1860, 2199
BseDI CCNNGG 7 cut(s) 764, 927, 1740, 1810, 1847, 2042, 2364
BseGI GGATG 9 cut(s) 332, 511, 593, 1017, 1605, 1843, 1881, 2053, 2194
BseJI GATNNNNATC 1 cut(s) 1173
BseLI CCNNNNNNNGG 9 cut(s) 171, 563, 652, 725, 759, 1481, 1895, 1896, 2166
BseMI GCAATG 4 cut(s) 213, 394, 1535, 1936
BseMII CTCAG 8 cut(s) 457, 476, 833, 1211, 1403, 1550, 1682, 1699
BseNI ACTGG 6 cut(s) 412, 764, 849, 1519, 1571, 1677
BseRI GAGGAG 2 cut(s) 1398, 2381
BseSI GKGCMC 1 cut(s) 1186
BseXI GCAGC 5 cut(s) 48, 1257, 1260, 1768, 1850
BseYI CCCAGC 1 cut(s) 66
BsgI GTGCAG 2 cut(s) 256, 1629
Bsh1285I CGRYCG 2 cut(s) 51, 2364
BshFI GGCC 8 cut(s) 656, 715, 763, 933, 994, 1775, 2159, 2202
BshTI ACCGGT 1 cut(s) 2297
BsiEI CGRYCG 2 cut(s) 51, 2364
BsiHKAI GWGCWC 5 cut(s) 47, 184, 882, 1186, 1643
BsiHKCI CYCGRG 2 cut(s) 40, 926
BsiSI CCGG 6 cut(s) 165, 327, 1292, 1507, 2016, 2298
BslFI GGGAC 1 cut(s) 2233
BslI CCNNNNNNNGG 9 cut(s) 171, 563, 652, 725, 759, 1481, 1895, 1896, 2166
BsmAI GTCTC 3 cut(s) 627, 1788, 2124
BsmFI GGGAC 1 cut(s) 2233
BsnI GGCC 8 cut(s) 656, 715, 763, 933, 994, 1775, 2159, 2202
Bso31I GGTCTC 1 cut(s) 627
BsoBI CYCGRG 2 cut(s) 40, 926
Bsp119I TTCGAA 2 cut(s) 1123, 1455
Bsp1286I GDGCHC 7 cut(s) 47, 184, 444, 882, 1186, 1537, 1643
Bsp13I TCCGGA 1 cut(s) 1291
Bsp1407I TGTACA 2 cut(s) 263, 1604
BspACI CCGC 4 cut(s) 33, 111, 1172, 2222
BspANI GGCC 8 cut(s) 656, 715, 763, 933, 994, 1775, 2159, 2202
BspCNI CTCAG 8 cut(s) 456, 475, 834, 1212, 1402, 1549, 1683, 1698
BspEI TCCGGA 1 cut(s) 1291
BspHI TCATGA 1 cut(s) 1434
BspLI GGNNCC 9 cut(s) 6, 288, 325, 1095, 1170, 1290, 2040, 2226, 2466
BspMI ACCTGC 1 cut(s) 727
BspT104I TTCGAA 2 cut(s) 1123, 1455
BspTNI GGTCTC 1 cut(s) 627
BsrDI GCAATG 4 cut(s) 213, 394, 1535, 1936
BsrFI RCCGGY 2 cut(s) 2015, 2297
BsrGI TGTACA 2 cut(s) 263, 1604
BsrI ACTGG 6 cut(s) 412, 764, 849, 1519, 1571, 1677
BssAI RCCGGY 2 cut(s) 2015, 2297
BssECI CCNNGG 7 cut(s) 764, 927, 1740, 1810, 1847, 2042, 2364
BssT1I CCWWGG 1 cut(s) 2042
Bst2UI CCWGG 7 cut(s) 564, 766, 1742, 1812, 1849, 1860, 2199
Bst4CI ACNGT 9 cut(s) 478, 699, 825, 1007, 1047, 1075, 1188, 1432, 1945
Bst6I CTCTTC 1 cut(s) 1377
BstAPI GCANNNNNTGC 4 cut(s) 1826, 1878, 1935, 2012
BstAUI TGTACA 2 cut(s) 263, 1604
BstBAI YACGTR 3 cut(s) 704, 1018, 1926
BstBI TTCGAA 2 cut(s) 1123, 1455
BstC8I GCNNGC 6 cut(s) 1062, 1226, 1268, 1907, 2031, 2220
BstENI CCTNNNNNAGG 1 cut(s) 723
BstF5I GGATG 9 cut(s) 332, 511, 593, 1017, 1605, 1843, 1881, 2053, 2194
BstMAI GTCTC 3 cut(s) 627, 1788, 2124
BstMCI CGRYCG 2 cut(s) 51, 2364
BstNI CCWGG 7 cut(s) 564, 766, 1742, 1812, 1849, 1860, 2199
BstNSI RCATGY 1 cut(s) 894
BstSCI CCNGG 8 cut(s) 325, 562, 764, 1740, 1810, 1847, 1858, 2197
BstSFI CTRYAG 1 cut(s) 1203
BstSLI GKGCMC 1 cut(s) 1186
BstV1I GCAGC 5 cut(s) 48, 1257, 1260, 1768, 1850
BstV2I GAAGAC 1 cut(s) 191
BstX2I RGATCY 5 cut(s) 4, 802, 1168, 1444, 2464
BstXI CCANNNNNNTGG 2 cut(s) 670, 1596
BstYI RGATCY 5 cut(s) 4, 802, 1168, 1444, 2464
Bsu36I CCTNAGG 1 cut(s) 1665
BsuRI GGCC 8 cut(s) 656, 715, 763, 933, 994, 1775, 2159, 2202
BtsCI GGATG 9 cut(s) 332, 511, 593, 1017, 1605, 1843, 1881, 2053, 2194
BtsI GCAGTG 1 cut(s) 268
BtsIMutI CAGTG 6 cut(s) 268, 349, 856, 1184, 1428, 1544
BveI ACCTGC 1 cut(s) 727
Cac8I GCNNGC 6 cut(s) 1062, 1226, 1268, 1907, 2031, 2220
CaiI CAGNNNCTG 3 cut(s) 521, 990, 1817
CciI TCATGA 1 cut(s) 1434
Cfr10I RCCGGY 2 cut(s) 2015, 2297
Cfr13I GGNCC 7 cut(s) 323, 655, 762, 775, 931, 1093, 2039
CseI GACGC 1 cut(s) 185
Csp6I GTAC 4 cut(s) 264, 821, 1015, 1605
CspAI ACCGGT 1 cut(s) 2297
CviQI GTAC 4 cut(s) 264, 821, 1015, 1605
DraI TTTAAA 1 cut(s) 1960
DriI GACNNNNNGTC 1 cut(s) 1083
EaeI YGGCCR 4 cut(s) 713, 1773, 2157, 2200
Eam1104I CTCTTC 1 cut(s) 1377
Eam1105I GACNNNNNGTC 1 cut(s) 1083
EarI CTCTTC 1 cut(s) 1377
Ecl136II GAGCTC 1 cut(s) 45
Eco130I CCWWGG 1 cut(s) 2042
Eco24I GRGCYC 3 cut(s) 47, 444, 1537
Eco31I GGTCTC 1 cut(s) 627
Eco47I GGWCC 4 cut(s) 323, 775, 1093, 2039
Eco53kI GAGCTC 1 cut(s) 45
Eco57I CTGAAG 5 cut(s) 348, 744, 918, 1119, 2085
Eco72I CACGTG 2 cut(s) 704, 1926
Eco81I CCTNAGG 1 cut(s) 1665
Eco88I CYCGRG 2 cut(s) 40, 926
EcoICRI GAGCTC 1 cut(s) 45
EcoNI CCTNNNNNAGG 1 cut(s) 723
EcoO109I RGGNCCY 2 cut(s) 931, 2039
EcoRI GAATTC 2 cut(s) 1451, 1700
EcoRII CCWGG 7 cut(s) 562, 764, 1740, 1810, 1847, 1858, 2197
EcoT14I CCWWGG 1 cut(s) 2042
EcoT22I ATGCAT 1 cut(s) 1874
EcoT38I GRGCYC 3 cut(s) 47, 444, 1537
ErhI CCWWGG 1 cut(s) 2042
FalI AAGNNNNNCTT 4 cut(s) 1092, 1124, 2168, 2200
FaqI GGGAC 1 cut(s) 2233
FauI CCCGC 1 cut(s) 104
FblI GTMKAC 2 cut(s) 2061, 2211
Fnu4HI GCNGC 6 cut(s) 34, 37, 1271, 1274, 1782, 1864
FokI GGATG 9 cut(s) 319, 518, 580, 1024, 1612, 1830, 1868, 2060, 2181
FriOI GRGCYC 3 cut(s) 47, 444, 1537
Fsp4HI GCNGC 6 cut(s) 34, 37, 1271, 1274, 1782, 1864
FspBI CTAG 8 cut(s) 222, 588, 618, 1308, 1361, 2043, 2073, 2276
GluI GCNGC 6 cut(s) 34, 37, 1271, 1274, 1782, 1864
GsaI CCCAGC 1 cut(s) 70
GsuI CTGGAG 3 cut(s) 819, 1086, 1395
HaeIII GGCC 8 cut(s) 656, 715, 763, 933, 994, 1775, 2159, 2202
HapII CCGG 6 cut(s) 165, 327, 1292, 1507, 2016, 2298
HgaI GACGC 1 cut(s) 185
HincII GTYRAC 4 cut(s) 577, 1155, 1420, 1512
HindII GTYRAC 4 cut(s) 577, 1155, 1420, 1512
HindIII AAGCTT 3 cut(s) 404, 1106, 1370
HinfI GANTC 8 cut(s) 87, 161, 226, 739, 946, 987, 1751, 2135
HpaI GTTAAC 1 cut(s) 1420
HpaII CCGG 6 cut(s) 165, 327, 1292, 1507, 2016, 2298
HphI GGTGA 5 cut(s) 284, 1226, 1378, 2331, 2447
Hpy99I CGWCG 1 cut(s) 1956
HpyCH4III ACNGT 9 cut(s) 478, 699, 825, 1007, 1047, 1075, 1188, 1432, 1945
HpyCH4IV ACGT 6 cut(s) 703, 1017, 1925, 1954, 1987, 1999
HpySE526I ACGT 6 cut(s) 703, 1017, 1925, 1954, 1987, 1999
Kpn2I TCCGGA 1 cut(s) 1291
KspAI GTTAAC 1 cut(s) 1420
LmnI GCTCC 3 cut(s) 614, 885, 2224
Lsp1109I GCAGC 5 cut(s) 48, 1257, 1260, 1768, 1850
MaeI CTAG 8 cut(s) 222, 588, 618, 1308, 1361, 2043, 2073, 2276
MaeII ACGT 6 cut(s) 703, 1017, 1925, 1954, 1987, 1999
MaeIII GTNAC 7 cut(s) 101, 699, 744, 783, 1030, 1075, 2314
MboII GAAGA 9 cut(s) 70, 191, 1394, 1469, 1478, 1746, 1993, 2246, 2450
MfeI CAATTG 2 cut(s) 816, 1196
MflI RGATCY 5 cut(s) 4, 802, 1168, 1444, 2464
MhlI GDGCHC 7 cut(s) 47, 184, 444, 882, 1186, 1537, 1643
MlsI TGGCCA 1 cut(s) 1775
MluNI TGGCCA 1 cut(s) 1775
MlyI GAGTC 1 cut(s) 235
MmeI TCCRAC 3 cut(s) 149, 396, 1760
Mox20I TGGCCA 1 cut(s) 1775
Mph1103I ATGCAT 1 cut(s) 1874
MroI TCCGGA 1 cut(s) 1291
MroXI GAANNNNTTC 1 cut(s) 1680
MscI TGGCCA 1 cut(s) 1775
MseI TTAA 7 cut(s) 957, 1419, 1658, 1947, 1959, 2151, 2385
MslI CAYNNNNRTG 4 cut(s) 1179, 1433, 1594, 1924
Msp20I TGGCCA 1 cut(s) 1775
MspA1I CMGCKG 2 cut(s) 1415, 2222
MspI CCGG 6 cut(s) 165, 327, 1292, 1507, 2016, 2298
MspR9I CCNGG 8 cut(s) 327, 564, 766, 1742, 1812, 1849, 1860, 2199
MunI CAATTG 2 cut(s) 816, 1196
MvaI CCWGG 7 cut(s) 564, 766, 1742, 1812, 1849, 1860, 2199
NciI CCSGG 1 cut(s) 327
NlaIV GGNNCC 9 cut(s) 6, 288, 325, 1095, 1170, 1290, 2040, 2226, 2466
NmeAIII GCCGAG 1 cut(s) 2228
NmuCI GTSAC 3 cut(s) 101, 699, 1075
NsiI ATGCAT 1 cut(s) 1874
NspI RCATGY 1 cut(s) 894
NspV TTCGAA 2 cut(s) 1123, 1455
PaeR7I CTCGAG 1 cut(s) 40
PagI TCATGA 1 cut(s) 1434
PaqCI CACCTGC 1 cut(s) 727
PcsI WCGNNNNNNNCGW 2 cut(s) 576, 868
PdmI GAANNNNTTC 1 cut(s) 1680
PfeI GAWTC 7 cut(s) 87, 161, 739, 946, 987, 1751, 2135
PinAI ACCGGT 1 cut(s) 2297
PkrI GCNGC 6 cut(s) 35, 38, 1272, 1275, 1783, 1865
Ple19I CGATCG 1 cut(s) 51
PleI GAGTC 1 cut(s) 234
PmaCI CACGTG 2 cut(s) 704, 1926
PmlI CACGTG 2 cut(s) 704, 1926
PpsI GAGTC 1 cut(s) 234
Ppu21I YACGTR 3 cut(s) 704, 1018, 1926
PpuMI RGGWCCY 1 cut(s) 2039
Psp124BI GAGCTC 1 cut(s) 47
Psp5II RGGWCCY 1 cut(s) 2039
Psp6I CCWGG 7 cut(s) 562, 764, 1740, 1810, 1847, 1858, 2197
PspCI CACGTG 2 cut(s) 704, 1926
PspFI CCCAGC 1 cut(s) 66
PspGI CCWGG 7 cut(s) 562, 764, 1740, 1810, 1847, 1858, 2197
PspN4I GGNNCC 9 cut(s) 6, 288, 325, 1095, 1170, 1290, 2040, 2226, 2466
PspPI GGNCC 7 cut(s) 323, 655, 762, 775, 931, 1093, 2039
PspPPI RGGWCCY 1 cut(s) 2039
PspXI VCTCGAGB 1 cut(s) 40
PstNI CAGNNNCTG 3 cut(s) 521, 990, 1817
PsuI RGATCY 5 cut(s) 4, 802, 1168, 1444, 2464
PvuI CGATCG 1 cut(s) 51
PvuII CAGCTG 1 cut(s) 1415
RsaI GTAC 4 cut(s) 265, 822, 1016, 1606
RsaNI GTAC 4 cut(s) 264, 821, 1015, 1605
RseI CAYNNNNRTG 4 cut(s) 1179, 1433, 1594, 1924
SacI GAGCTC 1 cut(s) 47
SaqAI TTAA 7 cut(s) 957, 1419, 1658, 1947, 1959, 2151, 2385
SatI GCNGC 6 cut(s) 34, 37, 1271, 1274, 1782, 1864
Sau96I GGNCC 7 cut(s) 323, 655, 762, 775, 931, 1093, 2039
SchI GAGTC 1 cut(s) 235
ScrFI CCNGG 8 cut(s) 327, 564, 766, 1742, 1812, 1849, 1860, 2199
SduI GDGCHC 7 cut(s) 47, 184, 444, 882, 1186, 1537, 1643
SfcI CTRYAG 1 cut(s) 1203
Sfr274I CTCGAG 1 cut(s) 40
SfuI TTCGAA 2 cut(s) 1123, 1455
SinI GGWCC 4 cut(s) 323, 775, 1093, 2039
SlaI CTCGAG 1 cut(s) 40
SmiMI CAYNNNNRTG 4 cut(s) 1179, 1433, 1594, 1924
SmlI CTYRAG 1 cut(s) 40
SmoI CTYRAG 1 cut(s) 40
SsiI CCGC 4 cut(s) 33, 111, 1172, 2222
SspI AATATT 1 cut(s) 1315
SspMI CTAG 8 cut(s) 222, 588, 618, 1308, 1361, 2043, 2073, 2276
SstI GAGCTC 1 cut(s) 47
StyD4I CCNGG 8 cut(s) 325, 562, 764, 1740, 1810, 1847, 1858, 2197
StyI CCWWGG 1 cut(s) 2042
TaaI ACNGT 9 cut(s) 478, 699, 825, 1007, 1047, 1075, 1188, 1432, 1945
TaiI ACGT 6 cut(s) 706, 1020, 1928, 1957, 1990, 2002
TaqI TCGA 7 cut(s) 41, 47, 51, 1049, 1123, 1440, 1455
TaqII GACCGA 1 cut(s) 2378
TatI WGTACW 2 cut(s) 263, 1604
TauI GCSGC 1 cut(s) 36
TfiI GAWTC 7 cut(s) 87, 161, 739, 946, 987, 1751, 2135
Tru1I TTAA 7 cut(s) 957, 1419, 1658, 1947, 1959, 2151, 2385
Tru9I TTAA 7 cut(s) 957, 1419, 1658, 1947, 1959, 2151, 2385
TscAI CASTG 6 cut(s) 275, 349, 856, 1191, 1435, 1544
TseFI GTSAC 3 cut(s) 101, 699, 1075
TseI GCWGC 5 cut(s) 36, 1270, 1273, 1781, 1863
Tsp45I GTSAC 3 cut(s) 101, 699, 1075
TspGWI ACGGA 1 cut(s) 788
TspRI CASTG 6 cut(s) 275, 349, 856, 1191, 1435, 1544
VneI GTGCAC 1 cut(s) 1182
VpaK11BI GGWCC 4 cut(s) 323, 775, 1093, 2039
XagI CCTNNNNNAGG 1 cut(s) 723
XapI RAATTY 5 cut(s) 280, 679, 808, 1451, 1700
XbaI TCTAGA 2 cut(s) 1360, 2275
XceI RCATGY 1 cut(s) 894
XcmI CCANNNNNNNNNTGG 1 cut(s) 1892
XhoI CTCGAG 1 cut(s) 40
XmaJI CCTAGG 1 cut(s) 2042
XmiI GTMKAC 2 cut(s) 2061, 2211
XmnI GAANNNNTTC 1 cut(s) 1680
XspI CTAG 8 cut(s) 222, 588, 618, 1308, 1361, 2043, 2073, 2276
Zsp2I ATGCAT 1 cut(s) 1874
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.