RchiOBHm_Chr3g0460501

B3 domain-containing

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0001663
Physical Location & Seq
Forward (+)
8575134 .. 8581194
6061 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ42701

Sequence Viewer

Length: 2616 bp
ATGGATCCGAGTGCTTGTATGAACGCGTATTGCGGGAGCTCGAGCTCGATCGAATGGAAAAAAGGCTGGGCTTTGCGATCTGGTAGATTCGCCAATCTGTGTCACAAGTGCGGGACTGCATATGAGGAATCACTTTTTTGTGATGTTTTCCATTCAAAAGAATCTGGTTGGAGGGAGTGCGCTCAATGTGGAAAGCATCTTCATTGTGGGTGCATTGCTTCCAGGAGTCTGCTTGATTTCCTTGACAGTGGTGGTGTCAAGTGTAAACACTGCACGAAAAATTCAGAACCTCACCCTATTGCTAGTGACGAAAAGCCTGATGGACCTGGCATATCCAAAATCAGTGATCTGAAATTAGCTCCTTCAGACAATCAATTGGATAGTAACAATGTCGATAAAGTGAAGCTTATGCAGATGGAAAATAATAAAGAGTGTAATGGGCTCAGGAACTTGCTTCAATCTCAGAACAATGAAACAGTTGGGTTATTGCAGAAAATGAAACAGGATGATGTTCCAGCTCCTGTGGTTGAGATTGGAGGCACAAGTCTGTCAGTTTTTAATCAGACATCCAATGTATCATCTGAAAGTTCCAAACCAGAAATTTATAAAGCAAACTTGGGGATAAATGATATATATGAATCCCTTCCGCACACAAATTTGAGTATGAGCCTAGGTGCACCTGCAGGATATGCAAATCCTTTTCCTGGTATCGGTGTTGATGAACATGCTAGGACATCCTCTCTGTTCTTACAGGGAGCTAGGTCTCGTCATCTTTTGCCAAAGCCTCCAAAGTTGGCCCTTGCCACAGGTTTGGAGGAAAATTCTACAATGGCTTCACAGGCACGTGTTGCACGGCCACCTGCTGAAGGACGGGGCCGGAATCAGTTACTTCCCCGATACTGGCCCAGGATTACGGACCAAGAGTTGCAGCAAATATCTGGAGATTCAAATTCCACAATTGTACCATTGTTTGAAAAGATGTTGAGTGCCAGTGATGCGGGTCGTATTGGTCGTTTAGTGCTCCCTAAAGCATGTGCTGAAGCATATTTTCCTTCTATCTCTCAACCTGAGGGCCTTCCTTTAAGGATTCAAGATGTGAAGGGAAAAGAATGGGTGTTTCAGTTCAGATTCTGGCCTAACAATAATAGTAGGATGTATGTGTTAGAGGGTGTAACTCCCTGCATACAGTCAATGCAGTTGCAGGCTGGAGATACTGTGACTTTCAGTCGTATGGAACCTGAAGGAAAGCTTATTATGGGGTTTCGAAAGGCATCAAATTCTGTTGCAATGCAGTCAACGAAGGGCATGGATCCGCATCTAAGTGCACTGCCCAAACAATTAACTGCAGCTACTGGTGATCCGAGCTGGCATAAATCTGAAAAGCATGAAATCAGGACAAGGGAAGGCTTGCTGCTGCAATCGTTAGTGGTTCCGGAGAGAAAAAGAACTAGAAATATTGGATCCAAGAGTAAAAGGTTGCTCATTGATAGCCAAGATGCTCTAGAGCTGAAGCTTACTTGGGAAGAGGCTCAGGATTTGCTTCGTCCACCTCCAGCTGTTAAGCCAAGCACTGTCATGATCGAGGATCTTGAATTCGAAGAATATGAAGAACCTCCTGTGTTTGGAAAAAGGAGTATCTTCATAGTCCGGGCAACTGGGGAACATGAGCAATGGGCTCAGTGTGATGGTTGCTCTAAATGGCGAAGGTTGCCGGTTGATGTACTACTTCCATCTAAGTGGATGTGTACGGACAATGTTTGGGATCAAAACAGGTGTTCGTGCTCCGCACCAGATGAATTAACCCCTAGGGAACTGGAAAGTTTTCTCAGACTCAGTAAAGAATTCAAGAAAAGGAGAATGGCAACAAACCATAGGCCAATCCAGGAACATGAATCTTCTGGCCTTGATGCTTTGGCCAATGCTGCAATCCTTGGAGACAATGTGGCTGACCCAGGAACTGCATCAGTTGCTACAACAACAAAACATCCCAGGCATCGTCCTGGCTGCTCATGCATTGTGTGCATCCAGCCCCCAAGTGGGAAGGGCAAGCATAAGCCTTCATGCACATGCCAGGTGTGCATGACAGTTAAACGCCGTTTTAAAACCCTAATGATAAACAAGAAGAAACGTCAATCAGAACGTGAAGCAGAGATTGCCTGTAGAAACCAGCTTGCATGGGGCCCTAGGGATGAAGCAGAAGTAGACAGCACTTCTAGGCATGTATCATCACACCTTGATCCTTCAGACAATGAAGCCAAGTCTCCAAATGAATCAGAATCTAAGAGCCAACTTAAATTGGCGGAAAGTGGGAAGGGAAAGTTAGACTTGAATTGCCATCCTGGCCGAGAAGTAGACTTGCCAGCGGGGCCAAGTCAGTTGAGCATGATGTCCCTTCTTCAAGTAGCAACCCTTCCTCTACAGAGTTATTTGAAACAAACTGGTCTTACCAGCTTGGTAACTGAACAGCAGACAAGCTCAACATCACCTGTGCCGCTGCAAGCTACTGAGGGGAATGAGGAACAACTTAATGAGGATCAATGTGTTGTTTCTGTTGTTCAAGACGGGGAAAGTGGAGGTGACGAAAGGCAGGATCAAAGCCAAGAGGATCCTCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3 zf-CW

Protein Analysis

871

Amino Acids

96.23

Weight (kDa)

6.62

Isoelectric Point (pI)

59.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf_VAL1_N PF25813 49 - 97 9.8e-22 VAL1-like, N-terminal zinc finger domain
B3 PF02362 324 - 423 2.8e-15 B3 DNA binding domain
zf-CW PF07496 557 - 599 1.6e-10 CW-type Zinc Finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 606
AarI CACCTGC 2 cut(s) 688, 868
Acc36I ACCTGC 2 cut(s) 688, 868
AccI GTMKAC 2 cut(s) 2202, 2352
AccII CGCG 1 cut(s) 26
AccIII TCCGGA 1 cut(s) 1432
AciI CCGC 9 cut(s) 33, 111, 647, 998, 1313, 1785, 2300, 2363, 2492
AcoI YGGCCR 3 cut(s) 854, 1914, 2341
AcsI RAATTY 8 cut(s) 280, 600, 655, 820, 949, 1276, 1592, 1841
AcuI CTGAAG 6 cut(s) 348, 885, 1059, 1260, 1529, 2226
AcvI CACGTG 1 cut(s) 845
AfaI GTAC 3 cut(s) 963, 1722, 1747
AfiI CCNNNNNNNGG 7 cut(s) 704, 710, 866, 900, 1622, 2036, 2037
AflIII ACRYGT 2 cut(s) 24, 844
AhdI GACNNNNNGTC 1 cut(s) 1224
Alw21I GWGCWC 6 cut(s) 41, 47, 679, 1023, 1327, 1784
Alw26I GTCTC 3 cut(s) 768, 1929, 2265
Alw44I GTGCAC 2 cut(s) 675, 1323
AlwNI CAGNNNCTG 4 cut(s) 521, 1131, 1352, 1958
Ama87I CYCGRG 1 cut(s) 40
Aor13HI TCCGGA 1 cut(s) 1432
ApaI GGGCCC 1 cut(s) 2183
ApaLI GTGCAC 2 cut(s) 675, 1323
ApeKI GCWGC 7 cut(s) 928, 1346, 1411, 1414, 1922, 2004, 2494
ApoI RAATTY 8 cut(s) 280, 600, 655, 820, 949, 1276, 1592, 1841
Asp700I GAANNNNTTC 2 cut(s) 642, 1821
AspA2I CCTAGG 3 cut(s) 670, 1805, 2183
AspLEI GCGC 1 cut(s) 182
AspS9I GGNCC 9 cut(s) 323, 796, 874, 903, 916, 1072, 2179, 2180, 2366
AsuC2I CCSGG 1 cut(s) 1649
AsuHPI GGTGA 4 cut(s) 284, 1367, 2475, 2588
AsuII TTCGAA 2 cut(s) 1264, 1596
AvaI CYCGRG 1 cut(s) 40
AvaII GGWCC 2 cut(s) 323, 916
AvrII CCTAGG 3 cut(s) 670, 1805, 2183
AxyI CCTNAGG 1 cut(s) 1068
BaeGI GKGCMC 3 cut(s) 679, 1327, 2183
BaeI ACNNNNGTAYC 2 cut(s) 945, 978
BalI TGGCCA 1 cut(s) 1916
BamHI GGATCC 4 cut(s) 4, 1309, 1460, 2605
BanII GRGCYC 5 cut(s) 41, 47, 444, 1678, 2183
BarI GAAGNNNNNNTAC 2 cut(s) 2394, 2426
BbrPI CACGTG 1 cut(s) 845
Bbv12I GWGCWC 6 cut(s) 41, 47, 679, 1023, 1327, 1784
BbvI GCAGC 7 cut(s) 940, 1358, 1398, 1401, 1909, 1991, 2481
BccI CCATC 5 cut(s) 314, 409, 1679, 1738, 2343
BceAI ACGGC 2 cut(s) 869, 2079
BcnI CCSGG 1 cut(s) 1649
BcoDI GTCTC 3 cut(s) 768, 1929, 2265
BfaI CTAG 9 cut(s) 303, 671, 729, 759, 1449, 1502, 1806, 2184, 2214
BfmI CTRYAG 4 cut(s) 681, 1344, 2158, 2417
BfuAI ACCTGC 2 cut(s) 688, 868
BglI GCCNNNNNGGC 2 cut(s) 2340, 2365
BisI GCNGC 8 cut(s) 929, 1347, 1412, 1415, 1923, 2005, 2492, 2495
BlnI CCTAGG 3 cut(s) 670, 1805, 2183
BlsI GCNGC 8 cut(s) 930, 1348, 1413, 1416, 1924, 2006, 2493, 2496
Bme18I GGWCC 2 cut(s) 323, 916
BmeRI GACNNNNNGTC 1 cut(s) 1224
BmeT110I CYCGRG 1 cut(s) 40
BmgT120I GGNCC 9 cut(s) 323, 796, 874, 903, 916, 1072, 2179, 2180, 2366
BmrI ACTGGG 1 cut(s) 1665
BmsI GCATC 9 cut(s) 205, 985, 1280, 1324, 1486, 1897, 1970, 2002, 2031
BmuI ACTGGG 1 cut(s) 1665
BpmI CTGGAG 3 cut(s) 960, 1227, 1536
Bpu10I CCTNAGC 2 cut(s) 443, 1530
Bpu14I TTCGAA 2 cut(s) 1264, 1596
BpuMI CCSGG 1 cut(s) 1649
BsaAI YACGTR 1 cut(s) 845
BsaBI GATNNNNATC 1 cut(s) 1314
BsaI GGTCTC 1 cut(s) 768
BsaJI CCNNGG 7 cut(s) 670, 905, 1805, 1930, 1951, 1988, 2183
BsaWI WCCGGW 1 cut(s) 1432
BsaXI ACNNNNNCTCC 4 cut(s) 1200, 1230, 1926, 1956
Bsc4I CCNNNNNNNGG 7 cut(s) 704, 710, 866, 900, 1622, 2036, 2037
Bse118I RCCGGY 1 cut(s) 1711
Bse1I ACTGG 6 cut(s) 905, 990, 1357, 1660, 1818, 2443
Bse21I CCTNAGG 1 cut(s) 1068
Bse3DI GCAATG 3 cut(s) 213, 1293, 1676
Bse8I GATNNNNATC 1 cut(s) 1314
BseAI TCCGGA 1 cut(s) 1432
BseDI CCNNGG 7 cut(s) 670, 905, 1805, 1930, 1951, 1988, 2183
BseGI GGATG 9 cut(s) 511, 566, 734, 1158, 1746, 1984, 2022, 2194, 2335
BseJI GATNNNNATC 1 cut(s) 1314
BseLI CCNNNNNNNGG 7 cut(s) 704, 710, 866, 900, 1622, 2036, 2037
BseMI GCAATG 3 cut(s) 213, 1293, 1676
BseMII CTCAG 8 cut(s) 457, 476, 1059, 1544, 1691, 1840, 1846, 2496
BseNI ACTGG 6 cut(s) 905, 990, 1357, 1660, 1818, 2443
BseSI GKGCMC 3 cut(s) 679, 1327, 2183
BseXI GCAGC 7 cut(s) 940, 1358, 1398, 1401, 1909, 1991, 2481
BseYI CCCAGC 1 cut(s) 66
BsgI GTGCAG 1 cut(s) 256
Bsh1236I CGCG 1 cut(s) 26
Bsh1285I CGRYCG 1 cut(s) 51
BsiEI CGRYCG 1 cut(s) 51
BsiHKAI GWGCWC 6 cut(s) 41, 47, 679, 1023, 1327, 1784
BsiHKCI CYCGRG 1 cut(s) 40
BsiSI CCGG 4 cut(s) 877, 1433, 1648, 1712
BslFI GGGAC 2 cut(s) 127, 2374
BslI CCNNNNNNNGG 7 cut(s) 704, 710, 866, 900, 1622, 2036, 2037
BsmAI GTCTC 3 cut(s) 768, 1929, 2265
BsmFI GGGAC 2 cut(s) 127, 2374
Bso31I GGTCTC 1 cut(s) 768
BsoBI CYCGRG 1 cut(s) 40
Bsp119I TTCGAA 2 cut(s) 1264, 1596
Bsp120I GGGCCC 1 cut(s) 2179
Bsp1286I GDGCHC 9 cut(s) 41, 47, 444, 679, 1023, 1327, 1678, 1784, 2183
Bsp13I TCCGGA 1 cut(s) 1432
BspACI CCGC 9 cut(s) 33, 111, 647, 998, 1313, 1785, 2300, 2363, 2492
BspCNI CTCAG 8 cut(s) 456, 475, 1060, 1543, 1690, 1839, 1845, 2497
BspEI TCCGGA 1 cut(s) 1432
BspFNI CGCG 1 cut(s) 26
BspHI TCATGA 1 cut(s) 1575
BspMAI CTGCAG 2 cut(s) 685, 1348
BspMI ACCTGC 2 cut(s) 688, 868
BspT104I TTCGAA 2 cut(s) 1264, 1596
BspTNI GGTCTC 1 cut(s) 768
BsrDI GCAATG 3 cut(s) 213, 1293, 1676
BsrFI RCCGGY 1 cut(s) 1711
BsrI ACTGG 6 cut(s) 905, 990, 1357, 1660, 1818, 2443
BssAI RCCGGY 1 cut(s) 1711
BssECI CCNNGG 7 cut(s) 670, 905, 1805, 1930, 1951, 1988, 2183
BssT1I CCWWGG 4 cut(s) 670, 1805, 1930, 2183
Bst4CI ACNGT 6 cut(s) 248, 478, 1188, 1216, 1573, 2086
Bst6I CTCTTC 1 cut(s) 1518
BstAPI GCANNNNNTGC 5 cut(s) 689, 848, 1967, 2019, 2153
BstBAI YACGTR 1 cut(s) 845
BstBI TTCGAA 2 cut(s) 1264, 1596
BstC8I GCNNGC 7 cut(s) 1203, 1367, 1409, 2048, 2172, 2361, 2499
BstENI CCTNNNNNAGG 1 cut(s) 864
BstF5I GGATG 9 cut(s) 511, 566, 734, 1158, 1746, 1984, 2022, 2194, 2335
BstFNI CGCG 1 cut(s) 26
BstHHI GCGC 1 cut(s) 182
BstMAI GTCTC 3 cut(s) 768, 1929, 2265
BstMCI CGRYCG 1 cut(s) 51
BstNSI RCATGY 4 cut(s) 728, 1035, 2070, 2222
BstSFI CTRYAG 4 cut(s) 681, 1344, 2158, 2417
BstSLI GKGCMC 3 cut(s) 679, 1327, 2183
BstUI CGCG 1 cut(s) 26
BstV1I GCAGC 7 cut(s) 940, 1358, 1398, 1401, 1909, 1991, 2481
BstX2I RGATCY 5 cut(s) 4, 1309, 1460, 1585, 2605
BstXI CCANNNNNNTGG 2 cut(s) 811, 1737
BstYI RGATCY 5 cut(s) 4, 1309, 1460, 1585, 2605
Bsu36I CCTNAGG 1 cut(s) 1068
BtsCI GGATG 9 cut(s) 511, 566, 734, 1158, 1746, 1984, 2022, 2194, 2335
BtsI GCAGTG 2 cut(s) 268, 1325
BtsIMutI CAGTG 7 cut(s) 253, 268, 349, 997, 1325, 1569, 1685
BveI ACCTGC 2 cut(s) 688, 868
Cac8I GCNNGC 7 cut(s) 1203, 1367, 1409, 2048, 2172, 2361, 2499
CaiI CAGNNNCTG 4 cut(s) 521, 1131, 1352, 1958
CciI TCATGA 1 cut(s) 1575
CfoI GCGC 1 cut(s) 182
Cfr10I RCCGGY 1 cut(s) 1711
Cfr13I GGNCC 9 cut(s) 323, 796, 874, 903, 916, 1072, 2179, 2180, 2366
Csp6I GTAC 3 cut(s) 962, 1721, 1746
CviQI GTAC 3 cut(s) 962, 1721, 1746
DraI TTTAAA 1 cut(s) 2101
DriI GACNNNNNGTC 1 cut(s) 1224
EaeI YGGCCR 3 cut(s) 854, 1914, 2341
Eam1104I CTCTTC 1 cut(s) 1518
Eam1105I GACNNNNNGTC 1 cut(s) 1224
EarI CTCTTC 1 cut(s) 1518
EciI GGCGGA 1 cut(s) 2315
Ecl136II GAGCTC 2 cut(s) 39, 45
Eco130I CCWWGG 4 cut(s) 670, 1805, 1930, 2183
Eco24I GRGCYC 5 cut(s) 41, 47, 444, 1678, 2183
Eco31I GGTCTC 1 cut(s) 768
Eco47I GGWCC 2 cut(s) 323, 916
Eco53kI GAGCTC 2 cut(s) 39, 45
Eco57I CTGAAG 6 cut(s) 348, 885, 1059, 1260, 1529, 2226
Eco72I CACGTG 1 cut(s) 845
Eco81I CCTNAGG 1 cut(s) 1068
Eco88I CYCGRG 1 cut(s) 40
EcoICRI GAGCTC 2 cut(s) 39, 45
EcoNI CCTNNNNNAGG 1 cut(s) 864
EcoO109I RGGNCCY 3 cut(s) 1072, 2179, 2180
EcoRI GAATTC 2 cut(s) 1592, 1841
EcoT14I CCWWGG 4 cut(s) 670, 1805, 1930, 2183
EcoT22I ATGCAT 1 cut(s) 2015
EcoT38I GRGCYC 5 cut(s) 41, 47, 444, 1678, 2183
ErhI CCWWGG 4 cut(s) 670, 1805, 1930, 2183
FalI AAGNNNNNCTT 6 cut(s) 390, 422, 1233, 1265, 2309, 2341
FaqI GGGAC 2 cut(s) 127, 2374
FauI CCCGC 4 cut(s) 26, 104, 991, 2356
FauNDI CATATG 1 cut(s) 121
FblI GTMKAC 2 cut(s) 2202, 2352
Fnu4HI GCNGC 8 cut(s) 929, 1347, 1412, 1415, 1923, 2005, 2492, 2495
FokI GGATG 9 cut(s) 518, 553, 721, 1165, 1753, 1971, 2009, 2201, 2322
FriOI GRGCYC 5 cut(s) 41, 47, 444, 1678, 2183
Fsp4HI GCNGC 8 cut(s) 929, 1347, 1412, 1415, 1923, 2005, 2492, 2495
FspBI CTAG 9 cut(s) 303, 671, 729, 759, 1449, 1502, 1806, 2184, 2214
GlaI GCGC 1 cut(s) 181
GluI GCNGC 8 cut(s) 929, 1347, 1412, 1415, 1923, 2005, 2492, 2495
GsaI CCCAGC 1 cut(s) 70
GsuI CTGGAG 3 cut(s) 960, 1227, 1536
HapII CCGG 4 cut(s) 877, 1433, 1648, 1712
HhaI GCGC 1 cut(s) 182
Hin6I GCGC 1 cut(s) 180
HinP1I GCGC 1 cut(s) 180
HincII GTYRAC 1 cut(s) 1296
HindII GTYRAC 1 cut(s) 1296
HindIII AAGCTT 3 cut(s) 404, 1247, 1511
HpaII CCGG 4 cut(s) 877, 1433, 1648, 1712
HphI GGTGA 4 cut(s) 284, 1367, 2475, 2588
Hpy166II GTNNAC 8 cut(s) 266, 677, 1296, 1325, 1547, 1746, 2203, 2353
Hpy8I GTNNAC 8 cut(s) 266, 677, 1296, 1325, 1547, 1746, 2203, 2353
HpyCH4III ACNGT 6 cut(s) 248, 478, 1188, 1216, 1573, 2086
HpyCH4IV ACGT 3 cut(s) 844, 2128, 2140
HpySE526I ACGT 3 cut(s) 844, 2128, 2140
HspAI GCGC 1 cut(s) 180
Kpn2I TCCGGA 1 cut(s) 1432
LmnI GCTCC 6 cut(s) 36, 364, 523, 755, 1026, 1787
Lsp1109I GCAGC 7 cut(s) 940, 1358, 1398, 1401, 1909, 1991, 2481
LweI GCATC 9 cut(s) 205, 985, 1280, 1324, 1486, 1897, 1970, 2002, 2031
MaeI CTAG 9 cut(s) 303, 671, 729, 759, 1449, 1502, 1806, 2184, 2214
MaeII ACGT 3 cut(s) 844, 2128, 2140
MaeIII GTNAC 8 cut(s) 101, 305, 383, 885, 1171, 1216, 2455, 2576
MboII GAAGA 8 cut(s) 191, 1535, 1610, 1619, 1630, 1887, 2134, 2387
MfeI CAATTG 2 cut(s) 374, 957
MflI RGATCY 5 cut(s) 4, 1309, 1460, 1585, 2605
MhlI GDGCHC 9 cut(s) 41, 47, 444, 679, 1023, 1327, 1678, 1784, 2183
MlsI TGGCCA 1 cut(s) 1916
MluI ACGCGT 1 cut(s) 24
MluNI TGGCCA 1 cut(s) 1916
MlyI GAGTC 2 cut(s) 235, 1824
MmeI TCCRAC 1 cut(s) 149
Mox20I TGGCCA 1 cut(s) 1916
Mph1103I ATGCAT 1 cut(s) 2015
MroI TCCGGA 1 cut(s) 1432
MroXI GAANNNNTTC 2 cut(s) 642, 1821
MscI TGGCCA 1 cut(s) 1916
MseI TTAA 9 cut(s) 558, 1082, 1340, 1560, 1799, 2088, 2100, 2292, 2526
MslI CAYNNNNRTG 4 cut(s) 1320, 1574, 1735, 2065
Msp20I TGGCCA 1 cut(s) 1916
MspA1I CMGCKG 3 cut(s) 1556, 2363, 2494
MspI CCGG 4 cut(s) 877, 1433, 1648, 1712
MunI CAATTG 2 cut(s) 374, 957
MvnI CGCG 1 cut(s) 26
NciI CCSGG 1 cut(s) 1649
NdeI CATATG 1 cut(s) 121
NmeAIII GCCGAG 1 cut(s) 2369
NmuCI GTSAC 4 cut(s) 101, 305, 1216, 2576
NsiI ATGCAT 1 cut(s) 2015
NspI RCATGY 4 cut(s) 728, 1035, 2070, 2222
NspV TTCGAA 2 cut(s) 1264, 1596
PaeR7I CTCGAG 1 cut(s) 40
PagI TCATGA 1 cut(s) 1575
PaqCI CACCTGC 2 cut(s) 688, 868
PcsI WCGNNNNNNNCGW 1 cut(s) 1009
PdmI GAANNNNTTC 2 cut(s) 642, 1821
PfoI TCCNGGA 2 cut(s) 221, 1881
PkrI GCNGC 8 cut(s) 930, 1348, 1413, 1416, 1924, 2006, 2493, 2496
Ple19I CGATCG 1 cut(s) 51
PleI GAGTC 2 cut(s) 234, 1824
PmaCI CACGTG 1 cut(s) 845
PmlI CACGTG 1 cut(s) 845
PpsI GAGTC 2 cut(s) 234, 1824
Ppu21I YACGTR 1 cut(s) 845
PsiI TTATAA 1 cut(s) 606
Psp124BI GAGCTC 2 cut(s) 41, 47
PspCI CACGTG 1 cut(s) 845
PspFI CCCAGC 1 cut(s) 66
PspOMI GGGCCC 1 cut(s) 2179
PspPI GGNCC 9 cut(s) 323, 796, 874, 903, 916, 1072, 2179, 2180, 2366
PspXI VCTCGAGB 1 cut(s) 40
PstI CTGCAG 2 cut(s) 685, 1348
PstNI CAGNNNCTG 4 cut(s) 521, 1131, 1352, 1958
PsuI RGATCY 5 cut(s) 4, 1309, 1460, 1585, 2605
PvuI CGATCG 1 cut(s) 51
PvuII CAGCTG 1 cut(s) 1556
RsaI GTAC 3 cut(s) 963, 1722, 1747
RsaNI GTAC 3 cut(s) 962, 1721, 1746
RseI CAYNNNNRTG 4 cut(s) 1320, 1574, 1735, 2065
SacI GAGCTC 2 cut(s) 41, 47
SaqAI TTAA 9 cut(s) 558, 1082, 1340, 1560, 1799, 2088, 2100, 2292, 2526
SatI GCNGC 8 cut(s) 929, 1347, 1412, 1415, 1923, 2005, 2492, 2495
Sau96I GGNCC 9 cut(s) 323, 796, 874, 903, 916, 1072, 2179, 2180, 2366
SbfI CCTGCAGG 1 cut(s) 685
SchI GAGTC 2 cut(s) 235, 1824
SdaI CCTGCAGG 1 cut(s) 685
SduI GDGCHC 9 cut(s) 41, 47, 444, 679, 1023, 1327, 1678, 1784, 2183
SfaNI GCATC 9 cut(s) 205, 985, 1280, 1324, 1486, 1897, 1970, 2002, 2031
SfcI CTRYAG 4 cut(s) 681, 1344, 2158, 2417
Sfr274I CTCGAG 1 cut(s) 40
SfuI TTCGAA 2 cut(s) 1264, 1596
SinI GGWCC 2 cut(s) 323, 916
SlaI CTCGAG 1 cut(s) 40
SmiMI CAYNNNNRTG 4 cut(s) 1320, 1574, 1735, 2065
SmlI CTYRAG 1 cut(s) 40
SmoI CTYRAG 1 cut(s) 40
Sse8387I CCTGCAGG 1 cut(s) 685
SsiI CCGC 9 cut(s) 33, 111, 647, 998, 1313, 1785, 2300, 2363, 2492
SspI AATATT 1 cut(s) 1456
SspMI CTAG 9 cut(s) 303, 671, 729, 759, 1449, 1502, 1806, 2184, 2214
SstI GAGCTC 2 cut(s) 41, 47
StyI CCWWGG 4 cut(s) 670, 1805, 1930, 2183
TaaI ACNGT 6 cut(s) 248, 478, 1188, 1216, 1573, 2086
TaiI ACGT 3 cut(s) 847, 2131, 2143
TaqI TCGA 7 cut(s) 41, 47, 51, 393, 1264, 1581, 1596
TatI WGTACW 1 cut(s) 1720
TauI GCSGC 1 cut(s) 2494
Tru1I TTAA 9 cut(s) 558, 1082, 1340, 1560, 1799, 2088, 2100, 2292, 2526
Tru9I TTAA 9 cut(s) 558, 1082, 1340, 1560, 1799, 2088, 2100, 2292, 2526
TscAI CASTG 7 cut(s) 253, 275, 349, 997, 1332, 1576, 1685
TseFI GTSAC 4 cut(s) 101, 305, 1216, 2576
TseI GCWGC 7 cut(s) 928, 1346, 1411, 1414, 1922, 2004, 2494
Tsp45I GTSAC 4 cut(s) 101, 305, 1216, 2576
TspGWI ACGGA 2 cut(s) 929, 1763
TspRI CASTG 7 cut(s) 253, 275, 349, 997, 1332, 1576, 1685
VneI GTGCAC 2 cut(s) 675, 1323
VpaK11BI GGWCC 2 cut(s) 323, 916
XagI CCTNNNNNAGG 1 cut(s) 864
XapI RAATTY 8 cut(s) 280, 600, 655, 820, 949, 1276, 1592, 1841
XbaI TCTAGA 1 cut(s) 1501
XceI RCATGY 4 cut(s) 728, 1035, 2070, 2222
XcmI CCANNNNNNNNNTGG 1 cut(s) 2033
XhoI CTCGAG 1 cut(s) 40
XmaJI CCTAGG 3 cut(s) 670, 1805, 2183
XmiI GTMKAC 2 cut(s) 2202, 2352
XmnI GAANNNNTTC 2 cut(s) 642, 1821
XspI CTAG 9 cut(s) 303, 671, 729, 759, 1449, 1502, 1806, 2184, 2214
Zsp2I ATGCAT 1 cut(s) 2015
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.