Rorug03G0043100

B3 domain-containing

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0001663
Physical Location & Seq
Forward (+)
3322831 .. 3325515
2685 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0043100.1

Sequence Viewer

Length: 2685 bp
ATGGAGAAGATGACATTGGATTCGAAGGAATATGAGTTGAAGAAGAACAGTATGAGAAAGATGTACGAGAGCTTGCACTCCCAAGCATCTTCCGTGCTAGTCTTCACCCTTCAGTGGAAGGAACTGGAGGGATGCTTAGATTCGATCCGGGATGCGACCCAGACCCGACTCGAAGAGCTCCATGAACGAGAGCAGCAGTTGGATGCCAAAGAGTTAAAGATGGAGTCCAAAGCAAATGAGTTCCAGGAACGAGAGAAGCAGTTGGAGGCCAAAGAGTTAAAGATCCAGTCCGAAGTCAATGAGGCGTTTGGACTCGAAAAGCTGATTAAGGAGAAACAAAAAGAGGCTCTAGACAGCAAGAACTACTTGAATTCGCTCCAGTTATCGATCAAAGAGCATACCGAATCAGAGAAAGAGCTTGGAGCCAAGGAAAGACAAATAGAGAGACGAATTCAAGAATTGAATGGGGTTGAGAGGAATATAGAAGAGAAGTTGAAGAAATCTCGTGAGGAACTGAATCAGATTCAGAGGGGGATTAAGGAGCAGGGTGTAGAATGTGATTTGAAAGAAAAGCAAATTAAAGCGAAACAGGAGAAATTGAATCAGATTGAGAAGAATATCGAAGAGAAGTTGAAATTGGCAATTGAGGAGGAGAAGAAAGCATTTGATCTGAAACAAGAGCGTGTTAAAGAAGAGCAGAAACTGAGAGATGAGAGACTGCAAGAGGAGGAGAAGTTGAAAGAGCACAAGAAATCAGTAGTGGAAGCCTCTAAGACACTCAAGTCAAGAGAGGAAATAAAGAGGGAACTAGAACTGAAAGTGAAAGATCATTGCTCGCGAAAGAAATCAATGGAGGAGTGGTCCTGTAATCTCGAACGTAAAGAGAGAGAATTAGAATTGAAAGAGAAGCAAGTTTTGTCAAAAGCTGAGGAACTTGACTCGCTTAATAAAAGGACCCTCAATGAGTTTCAGTTGATGGAAAAGGAACTTAGTTCAGTCGAAAGTACGTTACAAGAACGTGAAAAGAAATTGGAAGCAGACTCCCAGCGACTTCAAGTGAAACAGAGGCAACTTGAAGTGAAGAGTGAAACAATGGAAAAGTTGCAGCAAGAATGCAAAAAGCAACTTGAAGCGAGAGAGAGACAACTTGAAGTAAAAAGTAATACAATGGAAAAATTGCATCAAGAACGCGAAAAGAAACTTGAAGTAAAAGAGAGACTACTTGAAGAGCAGAGCAAAGAGCTTGAAATGACAAAGAAACAATTTGGTTGTCACATGAAAGTCAAAAGTGAGCCACTGGAAAATACCCCAGCTGCTAACAATGCCATTGTTTCTGCATCTGCAAGTGATCATTCCAAAATCAATATCAGGGATGGTAGAGGCTTGCAACTATTCATGTATGAGCATATGAAGAGAAATGATTCAGTGAGCAAAGAAATCTCAGCTGTTCTTCAACAAGAGTCATTAGAGCCAGCAAAATTGGTTTTGGATGCAATGGAAGGGTTTCACCCTTCAAATTCGATTGTGGACAATAGGGAGTTTGATTTGGTAATTATTAGAAGGACATGTATTCTTTTGTTGGAAGAGTTAAAGAGAGTCTCGGCGCAAATTAATCCTCAGGTGAGAGGACAAGCAATGAAGTTGGCCGGTGATTGGAAGGCTAAGATGACGGTGGGGACTGAGAATTGGTTAGAGGTTTTGGGATTTTTGCGGCTTGTTACTACATATGAATTGACCTCTGCCTATGATGCAAATGAGCTTCAAGGTCTTCTTGATATAGTAGCTCAACATGAACAAGCAACTGAATTATCCCGAGCCCTTGGTACCACAGATAAGGCAGCTGCCAACAACGTTATTGTTGCATCTGTCAAAAATGAGAAAGCAGAATCGCCTGCACGAGCAACTTTTTCTTCTCCAATTCTTCAACCAACTGCCAAGAACAACATTATTGTTGCTCCTGTCACAACTAAGAAAGCTCCTGATGTCGAAACTAAGAATGCAAAAACTTCTGCAGCAGCAATCTTTTCTTCTCCTAATCTTCAACCAGCTACCACTGTCCCAGCTAATGCTGTTATTGCTATTCAGAATTTTATTACAGAGAAGAAACTGGTTAAGGCTTATGGACGCATTCACAAGTTCAAGTTATTAGACAAGTTCCCTCCAGCTCAAGTCTTAAAAGAATATGTTGAGAAGGCCATGAAGTGCATGGAAAATAGCGAGAGAAAAGAAATGCTCCATCAACAGGATCAGGTTGTAGACGAAGGAATATCTGATCTGCGACGTGCTATTGAATGCATCAAGCATCACAACCTTGAGTCTGAATACCCTTCTGAGAGCATTGAGAAACAAATAGTTGTGCTACAGAAATTTATGGAGGATCGGAGAAACCAAAATCAGAAAAAACGCTCATGCAGCACAATTGATTCCATCTTTCAACAGCAGGAACATAAATTTCAGCGGACATCCTTATCAGCGGGGAGACCATATGGATTGCACCCTGTCTATCCGAATCCAGGTTCATCATTTTACAATGGACAGTTTAGTATTCCTTCCAACAATCCCGTGCAGTTTAGCATGCCTGCCAACGGCGTTAATTATGAGTTCCCTAGAATATATCCGTTCCCTCCATACCCTCGCCCTCTTGTATATAGACACCCTGGGTATGGCCTTCAAAAGCCATATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3 zf-CW

Protein Analysis

894

Amino Acids

103.57

Weight (kDa)

8.03

Isoelectric Point (pI)

55.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Frigida PF07899 458 - 626 1.2e-33 Frigida-like protein
Frigida PF07899 694 - 797 3.1e-18 Frigida-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 781
Acc65I GGTACC 1 cut(s) 1823
AccB1I GGYRCC 1 cut(s) 1823
AccI GTMKAC 1 cut(s) 2256
AccII CGCG 2 cut(s) 838, 1191
AciI CCGC 3 cut(s) 1711, 2458, 2474
AclI AACGTT 1 cut(s) 1851
AclWI GGATC 4 cut(s) 139, 277, 2253, 2385
AcoI YGGCCR 1 cut(s) 1644
AcsI RAATTY 6 cut(s) 370, 450, 1516, 2086, 2366, 2450
AcuI CTGAAG 1 cut(s) 95
AfaI GTAC 3 cut(s) 65, 1006, 1825
AfiI CCNNNNNNNGG 6 cut(s) 114, 1653, 2242, 2513, 2585, 2663
AflIII ACRYGT 1 cut(s) 1565
AjiI CACGTC 1 cut(s) 2282
AjnI CCWGG 3 cut(s) 243, 2512, 2656
AjuI GAANNNNNNNTTGG 3 cut(s) 31, 620, 652
Alw21I GWGCWC 2 cut(s) 180, 747
Alw26I GTCTC 6 cut(s) 439, 709, 1135, 1210, 1603, 2473
AlwI GGATC 4 cut(s) 139, 277, 2253, 2385
AlwNI CAGNNNCTG 1 cut(s) 703
Ama87I CYCGRG 1 cut(s) 1812
AoxI GGCC 4 cut(s) 267, 1644, 2193, 2665
ApeKI GCWGC 8 cut(s) 193, 1105, 1313, 1838, 1841, 2012, 2015, 2412
ApoI RAATTY 6 cut(s) 370, 450, 1516, 2086, 2366, 2450
ArsI GACNNNNNNTTYG 1 cut(s) 36
AseI ATTAAT 1 cut(s) 1611
Asp700I GAANNNNTTC 2 cut(s) 1420, 1503
Asp718I GGTACC 1 cut(s) 1823
AspLEI GCGC 1 cut(s) 1606
AspS9I GGNCC 2 cut(s) 861, 954
AsuC2I CCSGG 1 cut(s) 149
AsuHPI GGTGA 4 cut(s) 97, 1499, 1633, 1661
AsuII TTCGAA 1 cut(s) 23
AvaI CYCGRG 1 cut(s) 1812
AvaII GGWCC 2 cut(s) 861, 954
AxyI CCTNAGG 1 cut(s) 1617
BanI GGYRCC 1 cut(s) 1823
BanII GRGCYC 2 cut(s) 180, 1819
BauI CACGAG 2 cut(s) 504, 1896
BbsI GAAGAC 2 cut(s) 94, 1760
Bbv12I GWGCWC 2 cut(s) 180, 747
BbvCI CCTCAGC 1 cut(s) 927
BbvI GCAGC 8 cut(s) 205, 1117, 1300, 1828, 1850, 2024, 2027, 2424
BccI CCATC 5 cut(s) 214, 970, 1367, 2244, 2435
BceAI ACGGC 1 cut(s) 2602
BciT130I CCWGG 3 cut(s) 245, 2514, 2658
BclI TGATCA 1 cut(s) 1348
BcnI CCSGG 1 cut(s) 149
BcoDI GTCTC 6 cut(s) 439, 709, 1135, 1210, 1603, 2473
BfaI CTAG 4 cut(s) 98, 350, 809, 2607
BfmI CTRYAG 2 cut(s) 2010, 2360
BisI GCNGC 9 cut(s) 194, 1106, 1314, 1712, 1839, 1842, 2013, 2016, 2413
BlsI GCNGC 9 cut(s) 195, 1107, 1315, 1713, 1840, 1843, 2014, 2017, 2414
Bme1390I CCNGG 4 cut(s) 149, 245, 2514, 2658
Bme18I GGWCC 2 cut(s) 861, 954
BmeT110I CYCGRG 1 cut(s) 1812
BmgBI CACGTC 1 cut(s) 2282
BmgT120I GGNCC 2 cut(s) 861, 954
BmiI GGNNCC 3 cut(s) 424, 956, 1825
BmrFI CCNGG 4 cut(s) 149, 245, 2514, 2658
BpiI GAAGAC 2 cut(s) 94, 1760
BpmI CTGGAG 3 cut(s) 146, 362, 2145
Bpu10I CCTNAGC 1 cut(s) 927
Bpu14I TTCGAA 1 cut(s) 23
BpuEI CTTGAG 3 cut(s) 764, 2151, 2333
BpuMI CCSGG 1 cut(s) 149
Bsa29I ATCGAT 1 cut(s) 386
BsaI GGTCTC 1 cut(s) 2473
BsaJI CCNNGG 4 cut(s) 426, 1819, 2656, 2657
BsaXI ACNNNNNCTCC 4 cut(s) 719, 749, 845, 875
Bsc4I CCNNNNNNNGG 6 cut(s) 114, 1653, 2242, 2513, 2585, 2663
Bse118I RCCGGY 1 cut(s) 1646
Bse1I ACTGG 5 cut(s) 129, 286, 379, 1302, 2112
Bse21I CCTNAGG 1 cut(s) 1617
Bse3DI GCAATG 3 cut(s) 829, 1500, 1641
BseBI CCWGG 3 cut(s) 245, 2514, 2658
BseCI ATCGAT 1 cut(s) 386
BseDI CCNNGG 4 cut(s) 426, 1819, 2656, 2657
BseGI GGATG 6 cut(s) 137, 157, 208, 1378, 1495, 2462
BseLI CCNNNNNNNGG 6 cut(s) 114, 1653, 2242, 2513, 2585, 2663
BseMI GCAATG 3 cut(s) 829, 1500, 1641
BseMII CTCAG 6 cut(s) 695, 918, 1455, 1631, 1671, 2322
BseNI ACTGG 5 cut(s) 129, 286, 379, 1302, 2112
BseRI GAGGAG 5 cut(s) 662, 665, 740, 743, 869
BseXI GCAGC 8 cut(s) 205, 1117, 1300, 1828, 1850, 2024, 2027, 2424
BseYI CCCAGC 3 cut(s) 1044, 1309, 2059
BsgI GTGCAG 2 cut(s) 1878, 2585
Bsh1236I CGCG 2 cut(s) 838, 1191
BshFI GGCC 4 cut(s) 269, 1646, 2195, 2667
BshNI GGYRCC 1 cut(s) 1823
BshVI ATCGAT 1 cut(s) 386
BsiHKAI GWGCWC 2 cut(s) 180, 747
BsiHKCI CYCGRG 1 cut(s) 1812
BsiSI CCGG 2 cut(s) 148, 1647
BslFI GGGAC 2 cut(s) 1690, 2042
BslI CCNNNNNNNGG 6 cut(s) 114, 1653, 2242, 2513, 2585, 2663
BsmAI GTCTC 6 cut(s) 439, 709, 1135, 1210, 1603, 2473
BsmBI CGTCTC 1 cut(s) 439
BsmFI GGGAC 2 cut(s) 1690, 2042
BsmI GAATGC 4 cut(s) 1118, 2002, 2127, 2297
BsnI GGCC 4 cut(s) 269, 1646, 2195, 2667
Bso31I GGTCTC 1 cut(s) 2473
BsoBI CYCGRG 1 cut(s) 1812
Bsp119I TTCGAA 1 cut(s) 23
Bsp1286I GDGCHC 3 cut(s) 180, 747, 1819
Bsp143I GATC 9 cut(s) 144, 282, 387, 667, 826, 1348, 2245, 2272, 2377
Bsp68I TCGCGA 1 cut(s) 838
BspACI CCGC 3 cut(s) 1711, 2458, 2474
BspANI GGCC 4 cut(s) 269, 1646, 2195, 2667
BspCNI CTCAG 6 cut(s) 696, 919, 1454, 1630, 1672, 2323
BspDI ATCGAT 1 cut(s) 386
BspFNI CGCG 2 cut(s) 838, 1191
BspLI GGNNCC 3 cut(s) 424, 956, 1825
BspMAI CTGCAG 1 cut(s) 2014
BspPI GGATC 4 cut(s) 139, 277, 2253, 2385
BspQI GCTCTTC 3 cut(s) 168, 687, 1221
BspT104I TTCGAA 1 cut(s) 23
BspT107I GGYRCC 1 cut(s) 1823
BspTNI GGTCTC 1 cut(s) 2473
BsrDI GCAATG 3 cut(s) 829, 1500, 1641
BsrFI RCCGGY 1 cut(s) 1646
BsrI ACTGG 5 cut(s) 129, 286, 379, 1302, 2112
BssAI RCCGGY 1 cut(s) 1646
BssECI CCNNGG 4 cut(s) 426, 1819, 2656, 2657
BssMI GATC 9 cut(s) 144, 282, 387, 667, 826, 1348, 2245, 2272, 2377
BssSI CACGAG 2 cut(s) 504, 1896
BssT1I CCWWGG 2 cut(s) 426, 1819
Bst2BI CACGAG 2 cut(s) 504, 1896
Bst2UI CCWGG 3 cut(s) 245, 2514, 2658
Bst4CI ACNGT 4 cut(s) 50, 1672, 2056, 2538
Bst6I CTCTTC 8 cut(s) 168, 480, 618, 687, 1076, 1221, 1406, 1578
BstBI TTCGAA 1 cut(s) 23
BstC8I GCNNGC 7 cut(s) 74, 836, 1385, 1473, 1893, 2576, 2580
BstF5I GGATG 6 cut(s) 137, 157, 208, 1378, 1495, 2462
BstFNI CGCG 2 cut(s) 838, 1191
BstHHI GCGC 1 cut(s) 1606
BstKTI GATC 9 cut(s) 147, 285, 390, 670, 829, 1351, 2248, 2275, 2380
BstMAI GTCTC 6 cut(s) 439, 709, 1135, 1210, 1603, 2473
BstMBI GATC 9 cut(s) 144, 282, 387, 667, 826, 1348, 2245, 2272, 2377
BstMWI GCNNNNNNNGC 4 cut(s) 1322, 1748, 2075, 2412
BstNI CCWGG 3 cut(s) 245, 2514, 2658
BstNSI RCATGY 2 cut(s) 1569, 2578
BstSCI CCNGG 4 cut(s) 147, 243, 2512, 2656
BstSFI CTRYAG 2 cut(s) 2010, 2360
BstUI CGCG 2 cut(s) 838, 1191
BstV1I GCAGC 8 cut(s) 205, 1117, 1300, 1828, 1850, 2024, 2027, 2424
BstV2I GAAGAC 2 cut(s) 94, 1760
BstX2I RGATCY 1 cut(s) 282
BstYI RGATCY 1 cut(s) 282
Bsu15I ATCGAT 1 cut(s) 386
Bsu36I CCTNAGG 1 cut(s) 1617
BsuRI GGCC 4 cut(s) 269, 1646, 2195, 2667
BsuTUI ATCGAT 1 cut(s) 386
BtrI CACGTC 1 cut(s) 2282
BtsCI GGATG 6 cut(s) 137, 157, 208, 1378, 1495, 2462
BtsIMutI CAGTG 4 cut(s) 119, 1295, 1431, 2052
BtuMI TCGCGA 1 cut(s) 838
Cac8I GCNNGC 7 cut(s) 74, 836, 1385, 1473, 1893, 2576, 2580
CaiI CAGNNNCTG 1 cut(s) 703
CfoI GCGC 1 cut(s) 1606
Cfr10I RCCGGY 1 cut(s) 1646
Cfr13I GGNCC 2 cut(s) 861, 954
ClaI ATCGAT 1 cut(s) 386
CseI GACGC 1 cut(s) 2133
Csp6I GTAC 3 cut(s) 64, 1005, 1824
CviAII CATG 9 cut(s) 182, 1276, 1396, 1566, 1790, 2197, 2206, 2409, 2575
CviQI GTAC 3 cut(s) 64, 1005, 1824
DpnI GATC 9 cut(s) 146, 284, 389, 669, 828, 1350, 2247, 2274, 2379
DpnII GATC 9 cut(s) 144, 282, 387, 667, 826, 1348, 2245, 2272, 2377
DrdI GACNNNNNNGTC 1 cut(s) 781
DseDI GACNNNNNNGTC 1 cut(s) 781
EaeI YGGCCR 1 cut(s) 1644
Eam1104I CTCTTC 8 cut(s) 168, 480, 618, 687, 1076, 1221, 1406, 1578
EarI CTCTTC 8 cut(s) 168, 480, 618, 687, 1076, 1221, 1406, 1578
Ecl136II GAGCTC 1 cut(s) 178
Eco130I CCWWGG 2 cut(s) 426, 1819
Eco24I GRGCYC 2 cut(s) 180, 1819
Eco31I GGTCTC 1 cut(s) 2473
Eco47I GGWCC 2 cut(s) 861, 954
Eco53kI GAGCTC 1 cut(s) 178
Eco57I CTGAAG 1 cut(s) 95
Eco81I CCTNAGG 1 cut(s) 1617
Eco88I CYCGRG 1 cut(s) 1812
EcoICRI GAGCTC 1 cut(s) 178
EcoO109I RGGNCCY 1 cut(s) 954
EcoRI GAATTC 2 cut(s) 370, 450
EcoRII CCWGG 3 cut(s) 243, 2512, 2656
EcoT14I CCWWGG 2 cut(s) 426, 1819
EcoT22I ATGCAT 1 cut(s) 2297
EcoT38I GRGCYC 2 cut(s) 180, 1819
ErhI CCWWGG 2 cut(s) 426, 1819
Esp3I CGTCTC 1 cut(s) 439
FaeI CATG 9 cut(s) 185, 1279, 1399, 1569, 1793, 2200, 2209, 2412, 2578
FalI AAGNNNNNCTT 4 cut(s) 350, 382, 1755, 1787
FaqI GGGAC 2 cut(s) 1690, 2042
FatI CATG 9 cut(s) 181, 1275, 1395, 1565, 1789, 2196, 2205, 2408, 2574
FauI CCCGC 1 cut(s) 2467
FauNDI CATATG 3 cut(s) 1407, 1726, 2485
FbaI TGATCA 1 cut(s) 1348
FblI GTMKAC 1 cut(s) 2256
Fnu4HI GCNGC 9 cut(s) 194, 1106, 1314, 1712, 1839, 1842, 2013, 2016, 2413
FokI GGATG 6 cut(s) 144, 164, 215, 1385, 1502, 2449
FriOI GRGCYC 2 cut(s) 180, 1819
Fsp4HI GCNGC 9 cut(s) 194, 1106, 1314, 1712, 1839, 1842, 2013, 2016, 2413
FspBI CTAG 4 cut(s) 98, 350, 809, 2607
GlaI GCGC 1 cut(s) 1605
GluI GCNGC 9 cut(s) 194, 1106, 1314, 1712, 1839, 1842, 2013, 2016, 2413
GsaI CCCAGC 3 cut(s) 1048, 1313, 2063
GsuI CTGGAG 3 cut(s) 146, 362, 2145
HaeIII GGCC 4 cut(s) 269, 1646, 2195, 2667
HapII CCGG 2 cut(s) 148, 1647
HgaI GACGC 1 cut(s) 2133
HhaI GCGC 1 cut(s) 1606
Hin1II CATG 9 cut(s) 185, 1279, 1399, 1569, 1793, 2200, 2209, 2412, 2578
Hin6I GCGC 1 cut(s) 1604
HinP1I GCGC 1 cut(s) 1604
HpaII CCGG 2 cut(s) 148, 1647
HphI GGTGA 4 cut(s) 97, 1499, 1633, 1661
Hpy166II GTNNAC 2 cut(s) 1528, 2257
Hpy8I GTNNAC 2 cut(s) 1528, 2257
Hpy99I CGWCG 1 cut(s) 2283
HpyCH4III ACNGT 4 cut(s) 50, 1672, 2056, 2538
HpyCH4IV ACGT 5 cut(s) 877, 1007, 1018, 1851, 2281
HpyF10VI GCNNNNNNNGC 4 cut(s) 1322, 1748, 2075, 2412
HpySE526I ACGT 5 cut(s) 877, 1007, 1018, 1851, 2281
Hsp92II CATG 9 cut(s) 185, 1279, 1399, 1569, 1793, 2200, 2209, 2412, 2578
HspAI GCGC 1 cut(s) 1604
KpnI GGTACC 1 cut(s) 1827
Ksp22I TGATCA 1 cut(s) 1348
Kzo9I GATC 9 cut(s) 144, 282, 387, 667, 826, 1348, 2245, 2272, 2377
LguI GCTCTTC 3 cut(s) 168, 687, 1221
LmnI GCTCC 7 cut(s) 183, 381, 422, 541, 1960, 1981, 2238
Lsp1109I GCAGC 8 cut(s) 205, 1117, 1300, 1828, 1850, 2024, 2027, 2424
MaeI CTAG 4 cut(s) 98, 350, 809, 2607
MaeII ACGT 5 cut(s) 877, 1007, 1018, 1851, 2281
MaeIII GTNAC 4 cut(s) 1008, 1271, 1717, 1960
MalI GATC 9 cut(s) 146, 284, 389, 669, 828, 1350, 2247, 2274, 2379
MboI GATC 9 cut(s) 144, 282, 387, 667, 826, 1348, 2245, 2272, 2377
MfeI CAATTG 2 cut(s) 642, 2418
MflI RGATCY 1 cut(s) 282
MhlI GDGCHC 3 cut(s) 180, 747, 1819
MlyI GAGTC 8 cut(s) 162, 233, 306, 932, 1034, 1469, 1605, 2324
MmeI TCCRAC 4 cut(s) 180, 243, 1560, 2577
Mph1103I ATGCAT 1 cut(s) 2297
MroXI GAANNNNTTC 2 cut(s) 1420, 1503
MspA1I CMGCKG 5 cut(s) 1313, 1445, 1841, 2458, 2474
MspI CCGG 2 cut(s) 148, 1647
MspR9I CCNGG 4 cut(s) 149, 245, 2514, 2658
MunI CAATTG 2 cut(s) 642, 2418
Mva1269I GAATGC 4 cut(s) 1118, 2002, 2127, 2297
MvaI CCWGG 3 cut(s) 245, 2514, 2658
MvnI CGCG 2 cut(s) 838, 1191
MwoI GCNNNNNNNGC 4 cut(s) 1322, 1748, 2075, 2412
NciI CCSGG 1 cut(s) 149
NdeI CATATG 3 cut(s) 1407, 1726, 2485
NdeII GATC 9 cut(s) 144, 282, 387, 667, 826, 1348, 2245, 2272, 2377
NlaIII CATG 9 cut(s) 185, 1279, 1399, 1569, 1793, 2200, 2209, 2412, 2578
NlaIV GGNNCC 3 cut(s) 424, 956, 1825
NmeAIII GCCGAG 1 cut(s) 1580
NmuCI GTSAC 2 cut(s) 1271, 1960
NruI TCGCGA 1 cut(s) 838
NsiI ATGCAT 1 cut(s) 2297
NspI RCATGY 2 cut(s) 1569, 2578
NspV TTCGAA 1 cut(s) 23
PaeI GCATGC 1 cut(s) 2578
PasI CCCWGGG 1 cut(s) 2657
PciI ACATGT 1 cut(s) 1565
PciSI GCTCTTC 3 cut(s) 168, 687, 1221
PctI GAATGC 4 cut(s) 1118, 2002, 2127, 2297
PdmI GAANNNNTTC 2 cut(s) 1420, 1503
PfoI TCCNGGA 2 cut(s) 147, 243
PkrI GCNGC 9 cut(s) 195, 1107, 1315, 1713, 1840, 1843, 2014, 2017, 2414
PleI GAGTC 8 cut(s) 162, 232, 306, 932, 1034, 1468, 1604, 2323
PpsI GAGTC 8 cut(s) 162, 232, 306, 932, 1034, 1468, 1604, 2323
PpuMI RGGWCCY 1 cut(s) 954
PscI ACATGT 1 cut(s) 1565
PshBI ATTAAT 1 cut(s) 1611
Psp124BI GAGCTC 1 cut(s) 180
Psp1406I AACGTT 1 cut(s) 1851
Psp5II RGGWCCY 1 cut(s) 954
Psp6I CCWGG 3 cut(s) 243, 2512, 2656
PspFI CCCAGC 3 cut(s) 1044, 1309, 2059
PspGI CCWGG 3 cut(s) 243, 2512, 2656
PspN4I GGNNCC 3 cut(s) 424, 956, 1825
PspPI GGNCC 2 cut(s) 861, 954
PspPPI RGGWCCY 1 cut(s) 954
PstI CTGCAG 1 cut(s) 2014
PstNI CAGNNNCTG 1 cut(s) 703
PsuI RGATCY 1 cut(s) 282
PvuII CAGCTG 3 cut(s) 1313, 1445, 1841
RruI TCGCGA 1 cut(s) 838
RsaI GTAC 3 cut(s) 65, 1006, 1825
RsaNI GTAC 3 cut(s) 64, 1005, 1824
SacI GAGCTC 1 cut(s) 180
SapI GCTCTTC 3 cut(s) 168, 687, 1221
SatI GCNGC 9 cut(s) 194, 1106, 1314, 1712, 1839, 1842, 2013, 2016, 2413
Sau3AI GATC 9 cut(s) 144, 282, 387, 667, 826, 1348, 2245, 2272, 2377
Sau96I GGNCC 2 cut(s) 861, 954
SchI GAGTC 8 cut(s) 162, 233, 306, 932, 1034, 1469, 1605, 2324
ScrFI CCNGG 4 cut(s) 149, 245, 2514, 2658
SduI GDGCHC 3 cut(s) 180, 747, 1819
SfcI CTRYAG 2 cut(s) 2010, 2360
SfuI TTCGAA 1 cut(s) 23
SinI GGWCC 2 cut(s) 861, 954
SmlI CTYRAG 3 cut(s) 779, 2166, 2312
SmoI CTYRAG 3 cut(s) 779, 2166, 2312
SphI GCATGC 1 cut(s) 2578
SsiI CCGC 3 cut(s) 1711, 2458, 2474
SspMI CTAG 4 cut(s) 98, 350, 809, 2607
SstI GAGCTC 1 cut(s) 180
StyD4I CCNGG 4 cut(s) 147, 243, 2512, 2656
StyI CCWWGG 2 cut(s) 426, 1819
TaaI ACNGT 4 cut(s) 50, 1672, 2056, 2538
TaiI ACGT 5 cut(s) 880, 1010, 1021, 1854, 2284
TauI GCSGC 1 cut(s) 1714
TscAI CASTG 4 cut(s) 119, 1302, 1431, 2059
TseFI GTSAC 2 cut(s) 1271, 1960
TseI GCWGC 8 cut(s) 193, 1105, 1313, 1838, 1841, 2012, 2015, 2412
Tsp45I GTSAC 2 cut(s) 1271, 1960
TspDTI ATGAA 9 cut(s) 198, 1292, 1384, 1424, 1652, 1743, 1806, 2213, 2508
TspGWI ACGGA 2 cut(s) 82, 2607
TspRI CASTG 4 cut(s) 119, 1302, 1431, 2059
VpaK11BI GGWCC 2 cut(s) 861, 954
VspI ATTAAT 1 cut(s) 1611
XapI RAATTY 6 cut(s) 370, 450, 1516, 2086, 2366, 2450
XbaI TCTAGA 1 cut(s) 349
XceI RCATGY 2 cut(s) 1569, 2578
XcmI CCANNNNNNNNNTGG 1 cut(s) 2203
XmiI GTMKAC 1 cut(s) 2256
XmnI GAANNNNTTC 2 cut(s) 1420, 1503
XspI CTAG 4 cut(s) 98, 350, 809, 2607
Zsp2I ATGCAT 1 cut(s) 2297
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.