B3 domain-containing
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 648 |
| AarI | CACCTGC | 1 cut(s) | 910 |
| Acc36I | ACCTGC | 1 cut(s) | 910 |
| AccI | GTMKAC | 2 cut(s) | 2352, 2502 |
| AccII | CGCG | 2 cut(s) | 68, 317 |
| AccIII | TCCGGA | 1 cut(s) | 1582 |
| AciI | CCGC | 8 cut(s) | 75, 153, 689, 1040, 1463, 1697, 2450, 2513 |
| AcoI | YGGCCR | 3 cut(s) | 896, 2064, 2491 |
| AcsI | RAATTY | 8 cut(s) | 322, 642, 697, 862, 991, 1318, 1742, 1991 |
| AcuI | CTGAAG | 8 cut(s) | 390, 708, 927, 1101, 1302, 1424, 1679, 2376 |
| AcvI | CACGTG | 1 cut(s) | 887 |
| AfaI | GTAC | 2 cut(s) | 1872, 1897 |
| AfiI | CCNNNNNNNGG | 8 cut(s) | 213, 746, 752, 908, 942, 1772, 2186, 2187 |
| AflIII | ACRYGT | 2 cut(s) | 66, 886 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 1266 |
| AleI | CACNNNNGTG | 1 cut(s) | 1374 |
| Alw21I | GWGCWC | 7 cut(s) | 83, 721, 768, 1065, 1477, 1934, 2216 |
| Alw26I | GTCTC | 3 cut(s) | 810, 2079, 2415 |
| Alw44I | GTGCAC | 3 cut(s) | 717, 1473, 2212 |
| AlwNI | CAGNNNCTG | 4 cut(s) | 563, 1173, 1502, 2108 |
| Aor13HI | TCCGGA | 1 cut(s) | 1582 |
| ApaI | GGGCCC | 1 cut(s) | 2333 |
| ApaLI | GTGCAC | 3 cut(s) | 717, 1473, 2212 |
| ApeKI | GCWGC | 5 cut(s) | 970, 1561, 1564, 2072, 2154 |
| ApoI | RAATTY | 8 cut(s) | 322, 642, 697, 862, 991, 1318, 1742, 1991 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 684, 1971 |
| AspA2I | CCTAGG | 3 cut(s) | 712, 1955, 2333 |
| AspLEI | GCGC | 3 cut(s) | 224, 317, 1367 |
| AsuHPI | GGTGA | 5 cut(s) | 326, 1517, 2617, 2625, 2738 |
| AsuII | TTCGAA | 2 cut(s) | 1306, 1746 |
| AvaII | GGWCC | 4 cut(s) | 365, 958, 1276, 1611 |
| AvrII | CCTAGG | 3 cut(s) | 712, 1955, 2333 |
| AxyI | CCTNAGG | 1 cut(s) | 1110 |
| BaeGI | GKGCMC | 4 cut(s) | 721, 1477, 2216, 2333 |
| BalI | TGGCCA | 1 cut(s) | 2066 |
| BamHI | GGATCC | 3 cut(s) | 46, 1459, 2755 |
| BanII | GRGCYC | 4 cut(s) | 83, 486, 1828, 2333 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 2544, 2576 |
| BauI | CACGAG | 1 cut(s) | 1814 |
| BbrPI | CACGTG | 1 cut(s) | 887 |
| BbsI | GAAGAC | 2 cut(s) | 233, 1427 |
| Bbv12I | GWGCWC | 7 cut(s) | 83, 721, 768, 1065, 1477, 1934, 2216 |
| BbvI | GCAGC | 5 cut(s) | 982, 1548, 1551, 2059, 2141 |
| BccI | CCATC | 7 cut(s) | 37, 356, 451, 1350, 1829, 1888, 2493 |
| BceAI | ACGGC | 2 cut(s) | 911, 2229 |
| BcoDI | GTCTC | 3 cut(s) | 810, 2079, 2415 |
| BfmI | CTRYAG | 2 cut(s) | 2308, 2567 |
| BfoI | RGCGCY | 1 cut(s) | 1368 |
| BfuAI | ACCTGC | 1 cut(s) | 910 |
| BglI | GCCNNNNNGGC | 2 cut(s) | 2490, 2515 |
| BisI | GCNGC | 5 cut(s) | 971, 1562, 1565, 2073, 2155 |
| BlnI | CCTAGG | 3 cut(s) | 712, 1955, 2333 |
| BlsI | GCNGC | 5 cut(s) | 972, 1563, 1566, 2074, 2156 |
| Bme18I | GGWCC | 4 cut(s) | 365, 958, 1276, 1611 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 1266 |
| BmrI | ACTGGG | 1 cut(s) | 1815 |
| BmsI | GCATC | 8 cut(s) | 1027, 1322, 1474, 1636, 2047, 2120, 2152, 2181 |
| BmuI | ACTGGG | 1 cut(s) | 1815 |
| BpiI | GAAGAC | 2 cut(s) | 233, 1427 |
| BpmI | CTGGAG | 3 cut(s) | 1002, 1269, 1686 |
| Bpu10I | CCTNAGC | 2 cut(s) | 485, 1680 |
| Bpu14I | TTCGAA | 2 cut(s) | 1306, 1746 |
| BpuEI | CTTGAG | 2 cut(s) | 67, 1357 |
| BsaAI | YACGTR | 1 cut(s) | 887 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1464 |
| BsaI | GGTCTC | 1 cut(s) | 810 |
| BsaJI | CCNNGG | 7 cut(s) | 712, 947, 1955, 2080, 2101, 2138, 2333 |
| BsaWI | WCCGGW | 2 cut(s) | 206, 1582 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 1242, 1272, 2076, 2106 |
| Bsc4I | CCNNNNNNNGG | 8 cut(s) | 213, 746, 752, 908, 942, 1772, 2186, 2187 |
| Bse1I | ACTGG | 8 cut(s) | 947, 1032, 1507, 1810, 1862, 1968, 2027, 2593 |
| Bse21I | CCTNAGG | 1 cut(s) | 1110 |
| Bse3DI | GCAATG | 3 cut(s) | 255, 1335, 1826 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1464 |
| BseAI | TCCGGA | 1 cut(s) | 1582 |
| BseDI | CCNNGG | 7 cut(s) | 712, 947, 1955, 2080, 2101, 2138, 2333 |
| BseGI | GGATG | 9 cut(s) | 553, 608, 776, 1200, 1896, 2134, 2172, 2344, 2485 |
| BseJI | GATNNNNATC | 1 cut(s) | 1464 |
| BseLI | CCNNNNNNNGG | 8 cut(s) | 213, 746, 752, 908, 942, 1772, 2186, 2187 |
| BseMI | GCAATG | 3 cut(s) | 255, 1335, 1826 |
| BseMII | CTCAG | 8 cut(s) | 499, 518, 1101, 1694, 1841, 1990, 1996, 2646 |
| BseNI | ACTGG | 8 cut(s) | 947, 1032, 1507, 1810, 1862, 1968, 2027, 2593 |
| BseSI | GKGCMC | 4 cut(s) | 721, 1477, 2216, 2333 |
| BseXI | GCAGC | 5 cut(s) | 982, 1548, 1551, 2059, 2141 |
| BseYI | CCCAGC | 1 cut(s) | 108 |
| BsgI | GTGCAG | 1 cut(s) | 1920 |
| Bsh1236I | CGCG | 2 cut(s) | 68, 317 |
| Bsh1285I | CGRYCG | 1 cut(s) | 93 |
| BsiEI | CGRYCG | 1 cut(s) | 93 |
| BsiHKAI | GWGCWC | 7 cut(s) | 83, 721, 768, 1065, 1477, 1934, 2216 |
| BsiSI | CCGG | 3 cut(s) | 207, 919, 1583 |
| BslFI | GGGAC | 1 cut(s) | 2524 |
| BslI | CCNNNNNNNGG | 8 cut(s) | 213, 746, 752, 908, 942, 1772, 2186, 2187 |
| BsmAI | GTCTC | 3 cut(s) | 810, 2079, 2415 |
| BsmFI | GGGAC | 1 cut(s) | 2524 |
| BsmI | GAATGC | 1 cut(s) | 224 |
| Bso31I | GGTCTC | 1 cut(s) | 810 |
| Bsp119I | TTCGAA | 2 cut(s) | 1306, 1746 |
| Bsp120I | GGGCCC | 1 cut(s) | 2329 |
| Bsp13I | TCCGGA | 1 cut(s) | 1582 |
| BspACI | CCGC | 8 cut(s) | 75, 153, 689, 1040, 1463, 1697, 2450, 2513 |
| BspCNI | CTCAG | 8 cut(s) | 498, 517, 1102, 1693, 1840, 1989, 1995, 2647 |
| BspEI | TCCGGA | 1 cut(s) | 1582 |
| BspFNI | CGCG | 2 cut(s) | 68, 317 |
| BspMI | ACCTGC | 1 cut(s) | 910 |
| BspT104I | TTCGAA | 2 cut(s) | 1306, 1746 |
| BspTNI | GGTCTC | 1 cut(s) | 810 |
| BsrDI | GCAATG | 3 cut(s) | 255, 1335, 1826 |
| BsrI | ACTGG | 8 cut(s) | 947, 1032, 1507, 1810, 1862, 1968, 2027, 2593 |
| BssECI | CCNNGG | 7 cut(s) | 712, 947, 1955, 2080, 2101, 2138, 2333 |
| BssSI | CACGAG | 1 cut(s) | 1814 |
| BssT1I | CCWWGG | 4 cut(s) | 712, 1955, 2080, 2333 |
| Bst2BI | CACGAG | 1 cut(s) | 1814 |
| Bst4CI | ACNGT | 6 cut(s) | 290, 520, 1230, 1258, 1723, 2236 |
| Bst6I | CTCTTC | 1 cut(s) | 1668 |
| BstAPI | GCANNNNNTGC | 4 cut(s) | 890, 2117, 2169, 2303 |
| BstBAI | YACGTR | 1 cut(s) | 887 |
| BstBI | TTCGAA | 2 cut(s) | 1306, 1746 |
| BstC8I | GCNNGC | 7 cut(s) | 1245, 1517, 1559, 2198, 2322, 2511, 2649 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 906 |
| BstF5I | GGATG | 9 cut(s) | 553, 608, 776, 1200, 1896, 2134, 2172, 2344, 2485 |
| BstFNI | CGCG | 2 cut(s) | 68, 317 |
| BstH2I | RGCGCY | 1 cut(s) | 1368 |
| BstHHI | GCGC | 3 cut(s) | 224, 317, 1367 |
| BstMAI | GTCTC | 3 cut(s) | 810, 2079, 2415 |
| BstMCI | CGRYCG | 1 cut(s) | 93 |
| BstNSI | RCATGY | 3 cut(s) | 1077, 2220, 2372 |
| BstSFI | CTRYAG | 2 cut(s) | 2308, 2567 |
| BstSLI | GKGCMC | 4 cut(s) | 721, 1477, 2216, 2333 |
| BstUI | CGCG | 2 cut(s) | 68, 317 |
| BstV1I | GCAGC | 5 cut(s) | 982, 1548, 1551, 2059, 2141 |
| BstV2I | GAAGAC | 2 cut(s) | 233, 1427 |
| BstX2I | RGATCY | 4 cut(s) | 46, 1459, 1735, 2755 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 853, 1887 |
| BstYI | RGATCY | 4 cut(s) | 46, 1459, 1735, 2755 |
| Bsu36I | CCTNAGG | 1 cut(s) | 1110 |
| BtsCI | GGATG | 9 cut(s) | 553, 608, 776, 1200, 1896, 2134, 2172, 2344, 2485 |
| BtsI | GCAGTG | 3 cut(s) | 310, 1475, 2642 |
| BtsIMutI | CAGTG | 8 cut(s) | 295, 310, 391, 1039, 1475, 1719, 1835, 2642 |
| BveI | ACCTGC | 1 cut(s) | 910 |
| Cac8I | GCNNGC | 7 cut(s) | 1245, 1517, 1559, 2198, 2322, 2511, 2649 |
| CaiI | CAGNNNCTG | 4 cut(s) | 563, 1173, 1502, 2108 |
| CfoI | GCGC | 3 cut(s) | 224, 317, 1367 |
| CseI | GACGC | 2 cut(s) | 30, 227 |
| Csp6I | GTAC | 2 cut(s) | 1871, 1896 |
| CviQI | GTAC | 2 cut(s) | 1871, 1896 |
| DraI | TTTAAA | 1 cut(s) | 2251 |
| DriI | GACNNNNNGTC | 1 cut(s) | 1266 |
| EaeI | YGGCCR | 3 cut(s) | 896, 2064, 2491 |
| Eam1104I | CTCTTC | 1 cut(s) | 1668 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 1266 |
| EarI | CTCTTC | 1 cut(s) | 1668 |
| EciI | GGCGGA | 2 cut(s) | 1686, 2465 |
| Ecl136II | GAGCTC | 1 cut(s) | 81 |
| Eco130I | CCWWGG | 4 cut(s) | 712, 1955, 2080, 2333 |
| Eco24I | GRGCYC | 4 cut(s) | 83, 486, 1828, 2333 |
| Eco31I | GGTCTC | 1 cut(s) | 810 |
| Eco47I | GGWCC | 4 cut(s) | 365, 958, 1276, 1611 |
| Eco53kI | GAGCTC | 1 cut(s) | 81 |
| Eco57I | CTGAAG | 8 cut(s) | 390, 708, 927, 1101, 1302, 1424, 1679, 2376 |
| Eco72I | CACGTG | 1 cut(s) | 887 |
| Eco81I | CCTNAGG | 1 cut(s) | 1110 |
| EcoICRI | GAGCTC | 1 cut(s) | 81 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 906 |
| EcoO109I | RGGNCCY | 3 cut(s) | 1114, 2329, 2330 |
| EcoRI | GAATTC | 2 cut(s) | 1742, 1991 |
| EcoT14I | CCWWGG | 4 cut(s) | 712, 1955, 2080, 2333 |
| EcoT22I | ATGCAT | 1 cut(s) | 2165 |
| EcoT38I | GRGCYC | 4 cut(s) | 83, 486, 1828, 2333 |
| ErhI | CCWWGG | 4 cut(s) | 712, 1955, 2080, 2333 |
| FalI | AAGNNNNNCTT | 8 cut(s) | 432, 464, 1275, 1307, 1366, 1398, 2459, 2491 |
| FaqI | GGGAC | 1 cut(s) | 2524 |
| FauI | CCCGC | 4 cut(s) | 68, 146, 1033, 2506 |
| FauNDI | CATATG | 1 cut(s) | 163 |
| FblI | GTMKAC | 2 cut(s) | 2352, 2502 |
| Fnu4HI | GCNGC | 5 cut(s) | 971, 1562, 1565, 2073, 2155 |
| FokI | GGATG | 9 cut(s) | 560, 595, 763, 1207, 1903, 2121, 2159, 2351, 2472 |
| FriOI | GRGCYC | 4 cut(s) | 83, 486, 1828, 2333 |
| Fsp4HI | GCNGC | 5 cut(s) | 971, 1562, 1565, 2073, 2155 |
| GlaI | GCGC | 3 cut(s) | 223, 316, 1366 |
| GluI | GCNGC | 5 cut(s) | 971, 1562, 1565, 2073, 2155 |
| GsaI | CCCAGC | 1 cut(s) | 112 |
| GsuI | CTGGAG | 3 cut(s) | 1002, 1269, 1686 |
| HaeII | RGCGCY | 1 cut(s) | 1368 |
| HapII | CCGG | 3 cut(s) | 207, 919, 1583 |
| HgaI | GACGC | 2 cut(s) | 30, 227 |
| HhaI | GCGC | 3 cut(s) | 224, 317, 1367 |
| Hin6I | GCGC | 3 cut(s) | 222, 315, 1365 |
| HinP1I | GCGC | 3 cut(s) | 222, 315, 1365 |
| HincII | GTYRAC | 1 cut(s) | 1446 |
| HindII | GTYRAC | 1 cut(s) | 1446 |
| HindIII | AAGCTT | 4 cut(s) | 30, 446, 1289, 1661 |
| HpaII | CCGG | 3 cut(s) | 207, 919, 1583 |
| HphI | GGTGA | 5 cut(s) | 326, 1517, 2617, 2625, 2738 |
| Hpy166II | GTNNAC | 8 cut(s) | 308, 719, 1446, 1475, 1896, 2214, 2353, 2503 |
| Hpy8I | GTNNAC | 8 cut(s) | 308, 719, 1446, 1475, 1896, 2214, 2353, 2503 |
| HpyCH4III | ACNGT | 6 cut(s) | 290, 520, 1230, 1258, 1723, 2236 |
| HpyCH4IV | ACGT | 3 cut(s) | 886, 2278, 2290 |
| HpySE526I | ACGT | 3 cut(s) | 886, 2278, 2290 |
| HspAI | GCGC | 3 cut(s) | 222, 315, 1365 |
| Kpn2I | TCCGGA | 1 cut(s) | 1582 |
| LmnI | GCTCC | 5 cut(s) | 78, 406, 565, 797, 1068 |
| Lsp1109I | GCAGC | 5 cut(s) | 982, 1548, 1551, 2059, 2141 |
| LweI | GCATC | 8 cut(s) | 1027, 1322, 1474, 1636, 2047, 2120, 2152, 2181 |
| MaeII | ACGT | 3 cut(s) | 886, 2278, 2290 |
| MaeIII | GTNAC | 9 cut(s) | 143, 347, 425, 927, 1213, 1258, 1424, 2605, 2726 |
| MfeI | CAATTG | 2 cut(s) | 416, 999 |
| MflI | RGATCY | 4 cut(s) | 46, 1459, 1735, 2755 |
| MlsI | TGGCCA | 1 cut(s) | 2066 |
| MluI | ACGCGT | 1 cut(s) | 66 |
| MluNI | TGGCCA | 1 cut(s) | 2066 |
| MlyI | GAGTC | 2 cut(s) | 277, 1974 |
| MmeI | TCCRAC | 1 cut(s) | 191 |
| Mox20I | TGGCCA | 1 cut(s) | 2066 |
| Mph1103I | ATGCAT | 1 cut(s) | 2165 |
| MroI | TCCGGA | 1 cut(s) | 1582 |
| MroXI | GAANNNNTTC | 2 cut(s) | 684, 1971 |
| MscI | TGGCCA | 1 cut(s) | 2066 |
| MseI | TTAA | 9 cut(s) | 600, 1124, 1490, 1710, 1949, 2238, 2250, 2442, 2676 |
| MslI | CAYNNNNRTG | 4 cut(s) | 1374, 1470, 1724, 1885 |
| Msp20I | TGGCCA | 1 cut(s) | 2066 |
| MspA1I | CMGCKG | 2 cut(s) | 1706, 2513 |
| MspI | CCGG | 3 cut(s) | 207, 919, 1583 |
| MunI | CAATTG | 2 cut(s) | 416, 999 |
| Mva1269I | GAATGC | 1 cut(s) | 224 |
| MvnI | CGCG | 2 cut(s) | 68, 317 |
| NdeI | CATATG | 1 cut(s) | 163 |
| NmeAIII | GCCGAG | 1 cut(s) | 2519 |
| NmuCI | GTSAC | 5 cut(s) | 143, 347, 1258, 2605, 2726 |
| NsiI | ATGCAT | 1 cut(s) | 2165 |
| NspI | RCATGY | 3 cut(s) | 1077, 2220, 2372 |
| NspV | TTCGAA | 2 cut(s) | 1306, 1746 |
| OliI | CACNNNNGTG | 1 cut(s) | 1374 |
| PaqCI | CACCTGC | 1 cut(s) | 910 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 1051 |
| PctI | GAATGC | 1 cut(s) | 224 |
| PdmI | GAANNNNTTC | 2 cut(s) | 684, 1971 |
| PfoI | TCCNGGA | 2 cut(s) | 263, 2031 |
| PkrI | GCNGC | 5 cut(s) | 972, 1563, 1566, 2074, 2156 |
| Ple19I | CGATCG | 1 cut(s) | 93 |
| PleI | GAGTC | 2 cut(s) | 276, 1974 |
| PmaCI | CACGTG | 1 cut(s) | 887 |
| PmlI | CACGTG | 1 cut(s) | 887 |
| PpsI | GAGTC | 2 cut(s) | 276, 1974 |
| Ppu21I | YACGTR | 1 cut(s) | 887 |
| PsiI | TTATAA | 1 cut(s) | 648 |
| Psp124BI | GAGCTC | 1 cut(s) | 83 |
| PspCI | CACGTG | 1 cut(s) | 887 |
| PspFI | CCCAGC | 1 cut(s) | 108 |
| PspOMI | GGGCCC | 1 cut(s) | 2329 |
| PstNI | CAGNNNCTG | 4 cut(s) | 563, 1173, 1502, 2108 |
| PsuI | RGATCY | 4 cut(s) | 46, 1459, 1735, 2755 |
| PvuI | CGATCG | 1 cut(s) | 93 |
| PvuII | CAGCTG | 1 cut(s) | 1706 |
| RsaI | GTAC | 2 cut(s) | 1872, 1897 |
| RsaNI | GTAC | 2 cut(s) | 1871, 1896 |
| RseI | CAYNNNNRTG | 4 cut(s) | 1374, 1470, 1724, 1885 |
| SacI | GAGCTC | 1 cut(s) | 83 |
| SaqAI | TTAA | 9 cut(s) | 600, 1124, 1490, 1710, 1949, 2238, 2250, 2442, 2676 |
| SatI | GCNGC | 5 cut(s) | 971, 1562, 1565, 2073, 2155 |
| SchI | GAGTC | 2 cut(s) | 277, 1974 |
| SfaNI | GCATC | 8 cut(s) | 1027, 1322, 1474, 1636, 2047, 2120, 2152, 2181 |
| SfcI | CTRYAG | 2 cut(s) | 2308, 2567 |
| SfuI | TTCGAA | 2 cut(s) | 1306, 1746 |
| SinI | GGWCC | 4 cut(s) | 365, 958, 1276, 1611 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 1374, 1470, 1724, 1885 |
| SmlI | CTYRAG | 2 cut(s) | 82, 1372 |
| SmoI | CTYRAG | 2 cut(s) | 82, 1372 |
| SsiI | CCGC | 8 cut(s) | 75, 153, 689, 1040, 1463, 1697, 2450, 2513 |
| SspI | AATATT | 1 cut(s) | 1606 |
| SstI | GAGCTC | 1 cut(s) | 83 |
| StyI | CCWWGG | 4 cut(s) | 712, 1955, 2080, 2333 |
| TaaI | ACNGT | 6 cut(s) | 290, 520, 1230, 1258, 1723, 2236 |
| TaiI | ACGT | 3 cut(s) | 889, 2281, 2293 |
| TaqI | TCGA | 6 cut(s) | 35, 89, 93, 435, 1306, 1746 |
| TatI | WGTACW | 1 cut(s) | 1870 |
| Tru1I | TTAA | 9 cut(s) | 600, 1124, 1490, 1710, 1949, 2238, 2250, 2442, 2676 |
| Tru9I | TTAA | 9 cut(s) | 600, 1124, 1490, 1710, 1949, 2238, 2250, 2442, 2676 |
| TscAI | CASTG | 8 cut(s) | 295, 317, 391, 1039, 1482, 1726, 1835, 2649 |
| TseFI | GTSAC | 5 cut(s) | 143, 347, 1258, 2605, 2726 |
| TseI | GCWGC | 5 cut(s) | 970, 1561, 1564, 2072, 2154 |
| Tsp45I | GTSAC | 5 cut(s) | 143, 347, 1258, 2605, 2726 |
| TspGWI | ACGGA | 2 cut(s) | 971, 1913 |
| TspRI | CASTG | 8 cut(s) | 295, 317, 391, 1039, 1482, 1726, 1835, 2649 |
| VneI | GTGCAC | 3 cut(s) | 717, 1473, 2212 |
| VpaK11BI | GGWCC | 4 cut(s) | 365, 958, 1276, 1611 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 906 |
| XapI | RAATTY | 8 cut(s) | 322, 642, 697, 862, 991, 1318, 1742, 1991 |
| XbaI | TCTAGA | 1 cut(s) | 1651 |
| XceI | RCATGY | 3 cut(s) | 1077, 2220, 2372 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 2183 |
| XmaJI | CCTAGG | 3 cut(s) | 712, 1955, 2333 |
| XmiI | GTMKAC | 2 cut(s) | 2352, 2502 |
| XmnI | GAANNNNTTC | 2 cut(s) | 684, 1971 |
| Zsp2I | ATGCAT | 1 cut(s) | 2165 |