MD04G1174500.v1.1

B3 domain-containing

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0001663
Physical Location & Seq
Reverse (-)
26593508 .. 26598877
5370 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1174500.v1.1.491

Sequence Viewer

Length: 2697 bp
ATGAACGCAGCTTGCGGGACCTCGACGTCGATCGAGTGGAAGAGAGGTTGGGCTTTGCGATCTGGCGGATTCGCCAATCTCTGCATCAAGTGCTGGTCTGTATATGAGCAATCGATTTATTGTGATGTATACCACTCAGAGGAATCTGGTTGGAGGGAATGCGGAGTATGTGGCAAGCATCTACATTGTGGATGCATTGCTTCCACGTTACTGCTCGATCTTCTTGATGGTGGTGGTGTGAAGTGCATAAAATGTGCAAAGGATTCGGGACCTCACCCTATTTCAAGTGATGAAAAGCCTGATGGTCTTGGAACATCCAAAATCAGTGAACCGCAGTCAAATATTACAGACAATCAATTGGATGGACGTGATGTTGAAAAGTTGAAGCTTGTGCAGTTGGGAAATAATAAAGATTCTAATGGACTTATGAACTTGCTTCAACTTCGGAACGACAACACAAATGGGCTAATGCTCAAATTGAAACATGATGATGTTCCACCTCCTGGGGGTGAAATTGGAGGGGCATGTTTTTCAAATTTTAATCAGGCACCCCATGGATCTTCTGAAGCTTCCAAAGCAGAAGTTTTCAAGGCAAACTTAGGGATCAAGAATTTATATGAATCACTTCCGCATACAAATTTGAGTATGACCCTCGGTTCACCTTTGGGAAAAGCAAATCCTTTTCCTAGTGCCATTGTTGATGAAAGGGAACACAGCAAGACATCCTCTCCACTCCCACTAGGAGTTAGGCCTCAACATCTTTTTCCAAAGCCCCCAAAATTGGCCCTTTCCACAGGTTTGGAGGAAAAATCTACAATGGTTTCACATGTACGTGTGGCAAGGCCACCTGCCGAAGGACGGGGACGGAATCAGCTACTTCCCCGCTATTGGCCCAGGATTACAGACCAGGAATTACAGCAAATATCTGGAGATTCAAATTCCACAATTGTTCCCTTGTTTGAAAAGATGCTAAGTGCCAGTGATGCTGGTCGAATTGGTCGTTTGGTGCTTCCTAAAGCTTGTGCTGAGGCATATTTTCCTCCCATCTCTCAACCAGAGGGGCTTCCGTTAAGGATTCAAGATGTGAAGGGAAAAGAATGGATGTTTCAGTTCAGATTCTGGCCGAATAACAACAGTAGGATGTATGTTTTGGAGGGTGTAACCCCCTGCATACAGTCCATGCAGTTGCAGGCTGGAGATACTGTGACTTTTAGTCGTATGGATCCTGAAGGAAAACTAATTATGGGGTTTCGGAAAGCATCGAATACTGTTGCAATGCAGGATTCCCATCTTACTGCCATTCAGAATGGCCCTCACTCAAGTGAAACTTTGTTTTCGGGTGTTTTTGAGAACCTTCCTGTAATAAGTGGTTACCCTGGCCTTCTTCAGTCATTTAAGGGAAGCATGGATCCCCATCTAAATGCACTGTCGAAACACTTGACTACATCTAGTGGTGATATTAGCTGGAATAAAACTGAGAAGCAGGAAGGGAGGACAAGGGAGGGATTGCTGCTGCCGTCATTAGTTCCTGAGAGGAAAAGAACTAGAAATATAGGGTCCAAGAGTAAGAGGTTGCTCATTGATAACCAAGATGCTCTAGAGCTGAAGCTTACTTGGGAAGAGGCTCAGGATTTGCTTCGTCCACCTCCAGCTTCCAAGCCAAGCACTGTCGTGATTGAAGATCTTGAGTTTGAAGAATATGAAGAACCTCCAGTGTTTGGAAAGAGGAGTATTTTCACAGTCCGGTCAACTGGGGAACAAGAGCAATGGGTACAGTGTGATAGTTGCTCTAAATGGCGAAGATTGCCAGCTGATGCACTACTTTCATCCAAGTGGATATGTGCAGACAATGCTTGGGATCGAAGCAGGTCTTCGTGCTCTATGCCAGATGAATTGAGCCCAAGGGAACTGGAAAATTTTCTGAGAATGAGTAAAGAATTGAAGAAAAGGAGAATAGCGGCAGACCCTAGGCCAACCCCGGAGCATGAAGCTTCTGGCCTTGATGCTTTGGCCAATGCCGCAATCCTCGGAGACAGTGTGGCTGATCCAGAAGCTGCATTAGTTGCTACAACAACAAAACATCCCAGGCATCGTCCTGGCTGCTCATGCATTGTGTGCATTCAGCCCCCAAGTGGAAAGGGCAAACATAAGCCTACATGCACGTGCAACGTGTGCATGACAGTCAAACGCCGTTTTAAAACCATGATGATCAACAAGAAGAAACGTCAGTCGGAGCGGGAAGCAGAGATTGCATGCAGAAGCCAGCACACCTGGGCCCCTAGGGATGAAGCAGAAGTAGACAGCACTTCTAGGCTTGTATCGTCGCACGTTGATCCTTCAGATAACGAGGCCAGGTCTGCAAATGAATCAGAATCCAAGAGCCAAAGCAAATTGGCTGAAACCGGGAAGGGAATTTTAGACTTGAATTCCCATCCGGGCCGAGAAGGAGACTTGCAAGCAGGGCCAGACCATGTGAGTATGATGTCCCTTGTACAGGTTGCAACCCTTCCTCTTGAGACATATTTGAAACACAATGGTATTACCAGCTTGATATCTGAGCAACAGGAAAGTTCGACGTCACATGTTCCGCCACAAGTCGCTAATGAGACCGACGAACAACTTGACGATAATCACCGTCTGGAGCGAGAAACCGGGGGTGAAGAAAGGCCGGATCAAATCCAAGATGACCCTCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3 zf-CW

Protein Analysis

899

Amino Acids

99.1

Weight (kDa)

6.3

Isoelectric Point (pI)

52.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf_VAL1_N PF25813 43 - 90 3.1e-21 VAL1-like, N-terminal zinc finger domain
B3 PF02362 320 - 419 2.2e-15 B3 DNA binding domain
zf-CW PF07496 589 - 631 2.7e-12 CW-type Zinc Finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 856
AasI GACNNNNNNGTC 1 cut(s) 25
AatII GACGTC 2 cut(s) 29, 2579
Acc36I ACCTGC 2 cut(s) 856, 1857
AccB1I GGYRCC 1 cut(s) 547
AccB7I CCANNNNNTGG 2 cut(s) 503, 1718
AccBSI CCGCTC 1 cut(s) 2236
AccI GTMKAC 2 cut(s) 129, 2298
AcoI YGGCCR 2 cut(s) 1121, 2010
AcsI RAATTY 7 cut(s) 535, 610, 637, 937, 1915, 2412, 2425
AcuI CTGAAG 5 cut(s) 585, 1248, 1370, 1625, 2322
AcvI CACGTG 1 cut(s) 2163
AcyI GRCGYC 2 cut(s) 26, 2576
AfaI GTAC 3 cut(s) 831, 1773, 2493
AflIII ACRYGT 4 cut(s) 826, 832, 2169, 2581
AhdI GACNNNNNGTC 1 cut(s) 1212
AjiI CACGTC 1 cut(s) 368
AjnI CCWGG 8 cut(s) 502, 893, 906, 1375, 2084, 2095, 2270, 2351
AleI CACNNNNGTG 2 cut(s) 1320, 1670
Alw21I GWGCWC 1 cut(s) 1880
Alw26I GTCTC 4 cut(s) 2025, 2442, 2510, 2600
AlwNI CAGNNNCTG 2 cut(s) 1119, 2054
ApaI GGGCCC 1 cut(s) 2278
ApeKI GCWGC 5 cut(s) 8, 1510, 1513, 2054, 2100
ApoI RAATTY 7 cut(s) 535, 610, 637, 937, 1915, 2412, 2425
Asp700I GAANNNNTTC 2 cut(s) 624, 1917
AspA2I CCTAGG 2 cut(s) 1967, 2279
AsuC2I CCSGG 4 cut(s) 1979, 2404, 2436, 2653
AsuHPI GGTGA 6 cut(s) 266, 521, 651, 1466, 2624, 2669
AvaII GGWCC 3 cut(s) 18, 269, 1557
AvrII CCTAGG 2 cut(s) 1967, 2279
BaeGI GKGCMC 1 cut(s) 2278
BalI TGGCCA 1 cut(s) 2012
BamHI GGATCC 2 cut(s) 1222, 1408
BanI GGYRCC 1 cut(s) 547
BanII GRGCYC 2 cut(s) 1901, 2278
BbrPI CACGTG 1 cut(s) 2163
BbsI GAAGAC 1 cut(s) 1863
Bbv12I GWGCWC 1 cut(s) 1880
BbvCI CCTCAGC 1 cut(s) 1026
BbvI GCAGC 5 cut(s) 20, 1497, 1500, 2041, 2087
BccI CCATC 7 cut(s) 221, 296, 356, 1052, 1296, 1422, 2439
BceAI ACGGC 2 cut(s) 1501, 2175
BcgI CGANNNNNNTGC 2 cut(s) 246, 280
BciT130I CCWGG 8 cut(s) 504, 895, 908, 1377, 2086, 2097, 2272, 2353
BclI TGATCA 1 cut(s) 2208
BcnI CCSGG 4 cut(s) 1979, 2404, 2436, 2653
BcoDI GTCTC 4 cut(s) 2025, 2442, 2510, 2600
BfaI CTAG 8 cut(s) 687, 740, 1449, 1545, 1598, 1968, 2280, 2310
BfuAI ACCTGC 2 cut(s) 856, 1857
BglII AGATCT 1 cut(s) 1681
BisI GCNGC 7 cut(s) 9, 1511, 1514, 1959, 2019, 2055, 2101
BlnI CCTAGG 2 cut(s) 1967, 2279
BlsI GCNGC 7 cut(s) 10, 1512, 1515, 1960, 2020, 2056, 2102
Bme18I GGWCC 3 cut(s) 18, 269, 1557
BmeRI GACNNNNNGTC 1 cut(s) 1212
BmgBI CACGTC 1 cut(s) 368
BmiI GGNNCC 8 cut(s) 19, 270, 549, 1224, 1410, 1558, 2276, 2277
BmrI ACTGGG 1 cut(s) 1761
BmuI ACTGGG 1 cut(s) 1761
BpiI GAAGAC 1 cut(s) 1863
BpmI CTGGAG 5 cut(s) 948, 1215, 1632, 1695, 2660
Bpu10I CCTNAGC 2 cut(s) 1026, 1626
BpuEI CTTGAG 3 cut(s) 1303, 1706, 2534
BpuMI CCSGG 4 cut(s) 1979, 2404, 2436, 2653
Bsa29I ATCGAT 1 cut(s) 113
BsaAI YACGTR 2 cut(s) 833, 2163
BsaBI GATNNNNATC 2 cut(s) 1287, 1413
BsaHI GRCGYC 2 cut(s) 26, 2576
BsaI GGTCTC 1 cut(s) 2600
BsaWI WCCGGW 1 cut(s) 1743
BsaXI ACNNNNNCTCC 6 cut(s) 712, 742, 1188, 1218, 2022, 2052
Bse1I ACTGG 4 cut(s) 978, 1712, 1756, 1914
Bse3DI GCAATG 3 cut(s) 195, 1281, 1772
Bse8I GATNNNNATC 2 cut(s) 1287, 1413
BseBI CCWGG 8 cut(s) 504, 895, 908, 1377, 2086, 2097, 2272, 2353
BseCI ATCGAT 1 cut(s) 113
BseJI GATNNNNATC 2 cut(s) 1287, 1413
BseMI GCAATG 3 cut(s) 195, 1281, 1772
BseMII CTCAG 7 cut(s) 150, 1017, 1467, 1521, 1640, 1913, 2547
BseNI ACTGG 4 cut(s) 978, 1712, 1756, 1914
BseRI GAGGAG 1 cut(s) 1741
BseSI GKGCMC 1 cut(s) 2278
BseXI GCAGC 5 cut(s) 20, 1497, 1500, 2041, 2087
BsgI GTGCAG 2 cut(s) 413, 1863
Bsh1285I CGRYCG 1 cut(s) 33
BshNI GGYRCC 1 cut(s) 547
BshVI ATCGAT 1 cut(s) 113
BsiEI CGRYCG 1 cut(s) 33
BsiHKAI GWGCWC 1 cut(s) 1880
BsiSI CCGG 6 cut(s) 1744, 1979, 2403, 2435, 2652, 2669
BslFI GGGAC 4 cut(s) 31, 282, 876, 2470
BsmAI GTCTC 4 cut(s) 2025, 2442, 2510, 2600
BsmFI GGGAC 4 cut(s) 31, 282, 876, 2470
BsmI GAATGC 2 cut(s) 164, 2118
Bso31I GGTCTC 1 cut(s) 2600
Bsp120I GGGCCC 1 cut(s) 2274
Bsp1286I GDGCHC 3 cut(s) 1880, 1901, 2278
Bsp1407I TGTACA 1 cut(s) 2491
Bsp19I CCATGG 1 cut(s) 553
BspCNI CTCAG 7 cut(s) 149, 1018, 1468, 1522, 1639, 1914, 2548
BspDI ATCGAT 1 cut(s) 113
BspLI GGNNCC 8 cut(s) 19, 270, 549, 1224, 1410, 1558, 2276, 2277
BspMI ACCTGC 2 cut(s) 856, 1857
BspT107I GGYRCC 1 cut(s) 547
BspTNI GGTCTC 1 cut(s) 2600
BsrBI CCGCTC 1 cut(s) 2236
BsrDI GCAATG 3 cut(s) 195, 1281, 1772
BsrGI TGTACA 1 cut(s) 2491
BsrI ACTGG 4 cut(s) 978, 1712, 1756, 1914
BssNAI GTATAC 1 cut(s) 130
BssNI GRCGYC 2 cut(s) 26, 2576
BssT1I CCWWGG 4 cut(s) 553, 1901, 1967, 2279
Bst1107I GTATAC 1 cut(s) 130
Bst2UI CCWGG 8 cut(s) 504, 895, 908, 1377, 2086, 2097, 2272, 2353
Bst6I CTCTTC 2 cut(s) 35, 1614
BstACI GRCGYC 2 cut(s) 26, 2576
BstAPI GCANNNNNTGC 6 cut(s) 90, 1850, 2063, 2115, 2172, 2249
BstAUI TGTACA 1 cut(s) 2491
BstBAI YACGTR 2 cut(s) 833, 2163
BstC8I GCNNGC 7 cut(s) 13, 176, 1191, 1809, 2254, 2264, 2457
BstDEI CTNAG 9 cut(s) 136, 598, 971, 1026, 1476, 1530, 1626, 1922, 2556
BstDSI CCRYGG 1 cut(s) 553
BstEII GGTNACC 1 cut(s) 1370
BstENI CCTNNNNNAGG 2 cut(s) 852, 2492
BstMAI GTCTC 4 cut(s) 2025, 2442, 2510, 2600
BstMCI CGRYCG 1 cut(s) 33
BstNI CCWGG 8 cut(s) 504, 895, 908, 1377, 2086, 2097, 2272, 2353
BstNSI RCATGY 5 cut(s) 528, 830, 2160, 2256, 2585
BstPI GGTNACC 1 cut(s) 1370
BstSLI GKGCMC 1 cut(s) 2278
BstV1I GCAGC 5 cut(s) 20, 1497, 1500, 2041, 2087
BstV2I GAAGAC 1 cut(s) 1863
BstX2I RGATCY 4 cut(s) 557, 1222, 1408, 1681
BstXI CCANNNNNNTGG 1 cut(s) 799
BstYI RGATCY 4 cut(s) 557, 1222, 1408, 1681
BstZ17I GTATAC 1 cut(s) 130
Bsu15I ATCGAT 1 cut(s) 113
BsuTUI ATCGAT 1 cut(s) 113
BtgI CCRYGG 1 cut(s) 553
BtrI CACGTC 1 cut(s) 368
BtsIMutI CAGTG 7 cut(s) 331, 985, 1424, 1665, 1719, 1781, 2041
BveI ACCTGC 2 cut(s) 856, 1857
Cac8I GCNNGC 7 cut(s) 13, 176, 1191, 1809, 2254, 2264, 2457
CaiI CAGNNNCTG 2 cut(s) 1119, 2054
ClaI ATCGAT 1 cut(s) 113
Csp6I GTAC 3 cut(s) 830, 1772, 2492
CviQI GTAC 3 cut(s) 830, 1772, 2492
DdeI CTNAG 9 cut(s) 136, 598, 971, 1026, 1476, 1530, 1626, 1922, 2556
DraI TTTAAA 1 cut(s) 2197
DrdI GACNNNNNNGTC 1 cut(s) 25
DriI GACNNNNNGTC 1 cut(s) 1212
DseDI GACNNNNNNGTC 1 cut(s) 25
EaeI YGGCCR 2 cut(s) 1121, 2010
Eam1104I CTCTTC 2 cut(s) 35, 1614
Eam1105I GACNNNNNGTC 1 cut(s) 1212
EarI CTCTTC 2 cut(s) 35, 1614
EciI GGCGGA 2 cut(s) 81, 2577
Eco130I CCWWGG 4 cut(s) 553, 1901, 1967, 2279
Eco147I AGGCCT 1 cut(s) 751
Eco24I GRGCYC 2 cut(s) 1901, 2278
Eco31I GGTCTC 1 cut(s) 2600
Eco32I GATATC 1 cut(s) 2553
Eco47I GGWCC 3 cut(s) 18, 269, 1557
Eco57I CTGAAG 5 cut(s) 585, 1248, 1370, 1625, 2322
Eco72I CACGTG 1 cut(s) 2163
Eco91I GGTNACC 1 cut(s) 1370
EcoNI CCTNNNNNAGG 2 cut(s) 852, 2492
EcoO109I RGGNCCY 3 cut(s) 18, 269, 2275
EcoO65I GGTNACC 1 cut(s) 1370
EcoRI GAATTC 1 cut(s) 2425
EcoRII CCWGG 8 cut(s) 502, 893, 906, 1375, 2084, 2095, 2270, 2351
EcoRV GATATC 1 cut(s) 2553
EcoT14I CCWWGG 4 cut(s) 553, 1901, 1967, 2279
EcoT22I ATGCAT 2 cut(s) 197, 2111
EcoT38I GRGCYC 2 cut(s) 1901, 2278
ErhI CCWWGG 4 cut(s) 553, 1901, 1967, 2279
FalI AAGNNNNNCTT 6 cut(s) 581, 613, 1312, 1344, 1855, 1887
FaqI GGGAC 4 cut(s) 31, 282, 876, 2470
FauI CCCGC 3 cut(s) 8, 890, 2229
FbaI TGATCA 1 cut(s) 2208
FblI GTMKAC 2 cut(s) 129, 2298
Fnu4HI GCNGC 7 cut(s) 9, 1511, 1514, 1959, 2019, 2055, 2101
FriOI GRGCYC 2 cut(s) 1901, 2278
Fsp4HI GCNGC 7 cut(s) 9, 1511, 1514, 1959, 2019, 2055, 2101
FspBI CTAG 8 cut(s) 687, 740, 1449, 1545, 1598, 1968, 2280, 2310
GluI GCNGC 7 cut(s) 9, 1511, 1514, 1959, 2019, 2055, 2101
GsuI CTGGAG 5 cut(s) 948, 1215, 1632, 1695, 2660
HapII CCGG 6 cut(s) 1744, 1979, 2403, 2435, 2652, 2669
Hin1I GRCGYC 2 cut(s) 26, 2576
HincII GTYRAC 1 cut(s) 1749
HindII GTYRAC 1 cut(s) 1749
HindIII AAGCTT 5 cut(s) 386, 567, 1017, 1607, 1989
HpaII CCGG 6 cut(s) 1744, 1979, 2403, 2435, 2652, 2669
HphI GGTGA 6 cut(s) 266, 521, 651, 1466, 2624, 2669
Hpy166II GTNNAC 6 cut(s) 130, 329, 659, 1643, 1749, 2299
Hpy8I GTNNAC 6 cut(s) 130, 329, 659, 1643, 1749, 2299
Hpy99I CGWCG 5 cut(s) 28, 31, 2326, 2578, 2615
HpyCH4IV ACGT 9 cut(s) 26, 206, 367, 832, 2162, 2169, 2224, 2328, 2576
HpyF3I CTNAG 9 cut(s) 136, 598, 971, 1026, 1476, 1530, 1626, 1922, 2556
HpySE526I ACGT 9 cut(s) 26, 206, 367, 832, 2162, 2169, 2224, 2328, 2576
Hsp92I GRCGYC 2 cut(s) 26, 2576
Ksp22I TGATCA 1 cut(s) 2208
LmnI GCTCC 3 cut(s) 1981, 2233, 2641
Lsp1109I GCAGC 5 cut(s) 20, 1497, 1500, 2041, 2087
MaeI CTAG 8 cut(s) 687, 740, 1449, 1545, 1598, 1968, 2280, 2310
MaeII ACGT 9 cut(s) 26, 206, 367, 832, 2162, 2169, 2224, 2328, 2576
MaeIII GTNAC 5 cut(s) 207, 1159, 1204, 1370, 2577
MbiI CCGCTC 1 cut(s) 2236
MfeI CAATTG 2 cut(s) 356, 945
MflI RGATCY 4 cut(s) 557, 1222, 1408, 1681
MhlI GDGCHC 3 cut(s) 1880, 1901, 2278
MlsI TGGCCA 1 cut(s) 2012
MluNI TGGCCA 1 cut(s) 2012
MmeI TCCRAC 2 cut(s) 131, 2211
Mox20I TGGCCA 1 cut(s) 2012
Mph1103I ATGCAT 2 cut(s) 197, 2111
MroXI GAANNNNTTC 2 cut(s) 624, 1917
MscI TGGCCA 1 cut(s) 2012
MseI TTAA 4 cut(s) 540, 1070, 1395, 2196
MslI CAYNNNNRTG 7 cut(s) 489, 831, 1320, 1419, 1670, 1831, 2161
Msp20I TGGCCA 1 cut(s) 2012
MspA1I CMGCKG 1 cut(s) 1811
MspI CCGG 6 cut(s) 1744, 1979, 2403, 2435, 2652, 2669
MunI CAATTG 2 cut(s) 356, 945
Mva1269I GAATGC 2 cut(s) 164, 2118
MvaI CCWGG 8 cut(s) 504, 895, 908, 1377, 2086, 2097, 2272, 2353
NciI CCSGG 4 cut(s) 1979, 2404, 2436, 2653
NcoI CCATGG 1 cut(s) 553
NlaIV GGNNCC 8 cut(s) 19, 270, 549, 1224, 1410, 1558, 2276, 2277
NmeAIII GCCGAG 1 cut(s) 2465
NmuCI GTSAC 2 cut(s) 1204, 2577
NsiI ATGCAT 2 cut(s) 197, 2111
NspI RCATGY 5 cut(s) 528, 830, 2160, 2256, 2585
OliI CACNNNNGTG 2 cut(s) 1320, 1670
PaeI GCATGC 1 cut(s) 2256
PaqCI CACCTGC 1 cut(s) 856
PceI AGGCCT 1 cut(s) 751
PciI ACATGT 2 cut(s) 826, 2581
PcsI WCGNNNNNNNCGW 1 cut(s) 997
PctI GAATGC 2 cut(s) 164, 2118
PdmI GAANNNNTTC 2 cut(s) 624, 1917
PflMI CCANNNNNTGG 2 cut(s) 503, 1718
PkrI GCNGC 7 cut(s) 10, 1512, 1515, 1960, 2020, 2056, 2102
Ple19I CGATCG 1 cut(s) 33
PmaCI CACGTG 1 cut(s) 2163
PmlI CACGTG 1 cut(s) 2163
Ppu21I YACGTR 2 cut(s) 833, 2163
PpuMI RGGWCCY 2 cut(s) 18, 269
PscI ACATGT 2 cut(s) 826, 2581
Psp5II RGGWCCY 2 cut(s) 18, 269
Psp6I CCWGG 8 cut(s) 502, 893, 906, 1375, 2084, 2095, 2270, 2351
PspCI CACGTG 1 cut(s) 2163
PspEI GGTNACC 1 cut(s) 1370
PspGI CCWGG 8 cut(s) 502, 893, 906, 1375, 2084, 2095, 2270, 2351
PspN4I GGNNCC 8 cut(s) 19, 270, 549, 1224, 1410, 1558, 2276, 2277
PspOMI GGGCCC 1 cut(s) 2274
PspPPI RGGWCCY 2 cut(s) 18, 269
PstNI CAGNNNCTG 2 cut(s) 1119, 2054
PsuI RGATCY 4 cut(s) 557, 1222, 1408, 1681
PvuI CGATCG 1 cut(s) 33
PvuII CAGCTG 1 cut(s) 1811
RsaI GTAC 3 cut(s) 831, 1773, 2493
RsaNI GTAC 3 cut(s) 830, 1772, 2492
RseI CAYNNNNRTG 7 cut(s) 489, 831, 1320, 1419, 1670, 1831, 2161
SaqAI TTAA 4 cut(s) 540, 1070, 1395, 2196
SatI GCNGC 7 cut(s) 9, 1511, 1514, 1959, 2019, 2055, 2101
SduI GDGCHC 3 cut(s) 1880, 1901, 2278
SinI GGWCC 3 cut(s) 18, 269, 1557
SmiMI CAYNNNNRTG 7 cut(s) 489, 831, 1320, 1419, 1670, 1831, 2161
SmlI CTYRAG 3 cut(s) 1318, 1685, 2513
SmoI CTYRAG 3 cut(s) 1318, 1685, 2513
SphI GCATGC 1 cut(s) 2256
SseBI AGGCCT 1 cut(s) 751
SspI AATATT 1 cut(s) 343
SspMI CTAG 8 cut(s) 687, 740, 1449, 1545, 1598, 1968, 2280, 2310
StuI AGGCCT 1 cut(s) 751
StyI CCWWGG 4 cut(s) 553, 1901, 1967, 2279
TaiI ACGT 9 cut(s) 29, 209, 370, 835, 2165, 2172, 2227, 2331, 2579
TaqII GACCGA 1 cut(s) 2624
TatI WGTACW 1 cut(s) 2491
TauI GCSGC 2 cut(s) 1961, 2021
Tru1I TTAA 4 cut(s) 540, 1070, 1395, 2196
Tru9I TTAA 4 cut(s) 540, 1070, 1395, 2196
TscAI CASTG 7 cut(s) 331, 985, 1431, 1672, 1719, 1781, 2041
TseFI GTSAC 2 cut(s) 1204, 2577
TseI GCWGC 5 cut(s) 8, 1510, 1513, 2054, 2100
Tsp45I GTSAC 2 cut(s) 1204, 2577
TspGWI ACGGA 2 cut(s) 880, 1056
TspRI CASTG 7 cut(s) 331, 985, 1431, 1672, 1719, 1781, 2041
Van91I CCANNNNNTGG 2 cut(s) 503, 1718
VpaK11BI GGWCC 3 cut(s) 18, 269, 1557
XagI CCTNNNNNAGG 2 cut(s) 852, 2492
XapI RAATTY 7 cut(s) 535, 610, 637, 937, 1915, 2412, 2425
XbaI TCTAGA 1 cut(s) 1597
XceI RCATGY 5 cut(s) 528, 830, 2160, 2256, 2585
XmaJI CCTAGG 2 cut(s) 1967, 2279
XmiI GTMKAC 2 cut(s) 129, 2298
XmnI GAANNNNTTC 2 cut(s) 624, 1917
XspI CTAG 8 cut(s) 687, 740, 1449, 1545, 1598, 1968, 2280, 2310
ZraI GACGTC 2 cut(s) 27, 2577
Zsp2I ATGCAT 2 cut(s) 197, 2111
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.