MD12G1188100.v1.1

B3 domain-containing

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0001663
Physical Location & Seq
Reverse (-)
27007285 .. 27012760
5476 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1188100.v1.1.491

Sequence Viewer

Length: 2697 bp
ATGAACGCTGCTTGCGGGACCTCGACGTCGATCGAGTGGAAGAGAGGTTGGGCTTTGCGATCTGGCGGATTCGCCAATCTCTGTCTCAAGTGCTGGTCTGCGTATGAGCAATCAATTTATTGTGAGGTTTTCCACTCAAATGAATCCGGTTGGAGGGAGTGCGGAGTATGCGGCAAGCGTCTACATTGCGGATGCATTGCTTCCACGTTACTTCTTGATCTTCTTGACTGTGGTGGTGTGAAGTGCATAAAATGTGCAAAAGATTCGGGACCTCACCCTATTTCAAGTGATGAAAAGCCTGATGTTCTTGGCACGCCCAAAATGAGTGAGCCGCAGTCAAATATTACAAACAATCAATTGGATGGACATAGTGTTGAAAAGTTGAAGCTTGTGCAGTTGGGAAATAATAAAGATTGTAATGGACTTATGAACTTGCTTCAACTTCGGAACAATGACACAAAAGGGTTAATGCTCAAATGGAAACATGATGAGGTTCCACCTCTTGGGGGTGAAATTGGAGGCACATGTTTTTCAAATTTCAATCAGGCATCCAATGGATCAGCTGAAGCTTCCACAGCAGAAGCTTTCAAGGCAAACTTGGGGATAAATAATTTATATGAATCACTTCCACGTACGAATTTGAGTATGACCCTCGGTTCACCTTTGGGAAAAGAAAATCCTTTTCCTAGTGCCATTGTTGATGAAAGGGAACACAGCATGACATCCTCTCCACTCCCACCAGGAGCTAGGTCTCGACATCTTTTTCCAAAGGCCCCCAAATTGGCCCTTTCCACAGGTTTGGAGGAAAATTCTACAATGGTTTCACATGCACGTGTGGCAAGGCCACCTACCGAAGGACGGGGACGGAATCAGCTACTTCCCCGCTATTGGCCCAGGATTACAGACCAGGAATTACAGCAAATATCTGGAGATTCAAATTCTACAATTGTTCCTTTGTTTGAAAAAATGCTAAGTGCCAGTGATGCTGGTCGAATTGGTCGTTTGGTGCTCCCTAAAGCTTGTGCTGAAGCATATTTTCCTCCCATCTCTCAACCCGAGGGCCTTCCTTTAAGGATTCAAGATGTGAAGGGAAAAGAATGGGTGTTTCAATTCAGATTCTGGCCGAATAACAACAGTAGGATGTATGTTTTGGAGGGTGTAACGCCCTGCATACAGTCCATGCAGTTGCAGGCTGGAGATACTGTGACTTTCAGTCGTATGGATCCTGAAGGAAAACTAATTATGGGGTTTCGAAAAGCATCAAATACTGTTTCCATGCAGGATACCCATCTTACTGCCATTCAGAATGGCCCTCACTCAAGTGAAACTTTGTTTACGGGTGTTTTTGAGAACCTTCCTGTAATAAGTGGTTACCCTGGCCTTCTTCAGTCACTTAAGGGAAGCATGGATCCCCATCTAAATGCACTGTCGAAACACTTGACTACAGCTAGTGGTGATATTAGCTGGAATAAAACCGAGAAGCAGGAAGGGTGGACAAGGGAAGGATTGCTGATGCCATCATTAATTCCTGAGAGGAAAAGAACTAGAAATATAGGATCCAAGAGTAAGAGGATGCTCATTGATAGCCAAGATGCTCTAGAGCTGAAGCTTACTTGGGAAGAGGCTCAGGATTTGCTTCGTCCACCTCCAGCTTCCAAGCCAAGCACTGTCGTGATTGAGGATCTGGAGTTCGAAGAATATGAAGAACCTCCAGTGTTTGGAAAGAGGAGTATTTTCACAGTCCGGTCAACTGGGGAACAAGAGCAATGGGTGCAGTGTGATAGTTGCTCTAAATGGCGAAGATTGCCAGCTGATGCACTACTTTCATCTAAGTGGATATGCGCAGACAATGCTTGGGATCGAAGCAGGTCTTTGTGCTCTATACCAGATGAATTGAGCCCAAGGGAACTGGAAAATTTTCTCAGAATATGTAAGGAATCCAAGAAAAGGAGAATAGCGGCAGACCCTAGGCCAACCCCGGAGCACGAAGCTTCTGGCCTTGATGCTTTGGCCAATGCTGCAATACTCGGAGGCAGTGTGGCCAATCCAGAAGCTGCATCAGTTGCTACGACAACAAAACATCCCAGGCATCGTCCTGGCTGCTCATGCATTGTGTGCATCCAGCCCCCAAGTGGAAAGGGCAAACATAAGCCTACATGCACATGCAACGTGTGCTTGACCGTCAAACGCCGTTTCAAAACCATGATGATCAACAAGAAGAAACGCCAATCAGAGCGGGAAGCAGAGATTGCGTACAGAAGCCAGCACACATGGACCCCTAGGGATGAAGCAGAAGTAGACAGCACTTCTAGGCTTGTATCATCACACGTTGATCCTTCAGACAATGAGGCCAAGTCTGCAAATGAATCAGAAACCAAGAGCCAAAGCAAACTGGCTGAAACCGGGAAGGGAATACTAGACCTGAATTCCCATCCAGGCCGAGAAGGAGACTTACAAGCAGGGCCAGACCATGTGAGTATGATATCTCTTGTACAAGTAGCAACTCTTCCTCTTGAGACATATATGAAACACAATGGTATTACCAGCTTGATATCTGACCAACGGGAAAGTTCGACATCACATGTTCCCCCACAAGCCGCTAATGAGACCGAGGAACAACTCGATGAGAATCGCTGTCCAGAGCAGGAAAGCGGGGGTGAAAAAAGGCAGGATAAAAGCCTAGATGACCCTCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3 zf-CW

Protein Analysis

899

Amino Acids

99.08

Weight (kDa)

6.65

Isoelectric Point (pI)

50.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf_VAL1_N PF25813 43 - 90 5.8e-22 VAL1-like, N-terminal zinc finger domain
B3 PF02362 320 - 419 3.7e-15 B3 DNA binding domain
zf-CW PF07496 589 - 631 9.8e-12 CW-type Zinc Finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 25
AatII GACGTC 1 cut(s) 29
Acc16I TGCGCA 1 cut(s) 1843
Acc36I ACCTGC 1 cut(s) 1857
AccB7I CCANNNNNTGG 2 cut(s) 503, 1718
AccBSI CCGCTC 1 cut(s) 2236
AccI GTMKAC 2 cut(s) 181, 2298
AcoI YGGCCR 3 cut(s) 1121, 2010, 2040
AcsI RAATTY 6 cut(s) 535, 637, 808, 937, 1915, 2425
AcuI CTGAAG 6 cut(s) 585, 1047, 1248, 1370, 1625, 2322
AcvI CACGTG 1 cut(s) 833
AcyI GRCGYC 1 cut(s) 26
AfaI GTAC 3 cut(s) 634, 2255, 2493
AflII CTTAAG 1 cut(s) 1394
AflIII ACRYGT 5 cut(s) 524, 832, 2169, 2326, 2581
AhdI GACNNNNNGTC 1 cut(s) 1212
AjnI CCWGG 7 cut(s) 739, 893, 906, 1375, 2084, 2095, 2434
AleI CACNNNNGTG 2 cut(s) 1320, 1670
AloI GAACNNNNNNTCC 2 cut(s) 1673, 1705
Alw21I GWGCWC 3 cut(s) 1011, 1880, 1986
Alw26I GTCTC 5 cut(s) 89, 756, 2442, 2510, 2600
AlwNI CAGNNNCTG 2 cut(s) 1119, 2054
Ama87I CYCGRG 1 cut(s) 1055
ApeKI GCWGC 4 cut(s) 8, 2018, 2054, 2100
ApoI RAATTY 6 cut(s) 535, 637, 808, 937, 1915, 2425
AseI ATTAAT 1 cut(s) 1523
Asp700I GAANNNNTTC 2 cut(s) 624, 1917
AspA2I CCTAGG 2 cut(s) 1967, 2279
AspLEI GCGC 1 cut(s) 1844
AspS9I GGNCC 9 cut(s) 18, 269, 772, 784, 891, 1060, 1310, 2274, 2462
AsuC2I CCSGG 2 cut(s) 1979, 2404
AsuHPI GGTGA 5 cut(s) 266, 521, 651, 1466, 2669
AsuII TTCGAA 2 cut(s) 1252, 1692
AvaI CYCGRG 1 cut(s) 1055
AvaII GGWCC 3 cut(s) 18, 269, 2274
AvrII CCTAGG 2 cut(s) 1967, 2279
BalI TGGCCA 2 cut(s) 2012, 2042
BamHI GGATCC 3 cut(s) 1222, 1408, 1556
BanII GRGCYC 1 cut(s) 1901
BarI GAAGNNNNNNTAC 4 cut(s) 2436, 2468, 2490, 2522
BbrPI CACGTG 1 cut(s) 833
Bbv12I GWGCWC 3 cut(s) 1011, 1880, 1986
BbvI GCAGC 3 cut(s) 2005, 2041, 2087
BccI CCATC 6 cut(s) 356, 1052, 1296, 1422, 1525, 2439
BceAI ACGGC 1 cut(s) 2175
BcgI CGANNNNNNTGC 2 cut(s) 246, 280
BciT130I CCWGG 7 cut(s) 741, 895, 908, 1377, 2086, 2097, 2436
BciVI GTATCC 1 cut(s) 1276
BclI TGATCA 1 cut(s) 2208
BcnI CCSGG 2 cut(s) 1979, 2404
BcoDI GTCTC 5 cut(s) 89, 756, 2442, 2510, 2600
BfmI CTRYAG 1 cut(s) 1443
BfrI CTTAAG 1 cut(s) 1394
BfuAI ACCTGC 1 cut(s) 1857
BfuI GTATCC 1 cut(s) 1276
BisI GCNGC 8 cut(s) 9, 172, 332, 1959, 2019, 2055, 2101, 2598
BlnI CCTAGG 2 cut(s) 1967, 2279
BlsI GCNGC 8 cut(s) 10, 173, 333, 1960, 2020, 2056, 2102, 2599
Bme1390I CCNGG 9 cut(s) 741, 895, 908, 1377, 1979, 2086, 2097, 2404, 2436
Bme18I GGWCC 3 cut(s) 18, 269, 2274
BmeRI GACNNNNNGTC 1 cut(s) 1212
BmeT110I CYCGRG 1 cut(s) 1055
BmgT120I GGNCC 9 cut(s) 18, 269, 772, 784, 891, 1060, 1310, 2274, 2462
BmiI GGNNCC 8 cut(s) 19, 270, 495, 774, 1224, 1410, 1558, 2276
BmrFI CCNGG 9 cut(s) 741, 895, 908, 1377, 1979, 2086, 2097, 2404, 2436
BmrI ACTGGG 1 cut(s) 1761
BmuI ACTGGG 1 cut(s) 1761
BpmI CTGGAG 5 cut(s) 948, 1215, 1632, 1695, 1706
Bpu10I CCTNAGC 1 cut(s) 1626
Bpu14I TTCGAA 2 cut(s) 1252, 1692
BpuEI CTTGAG 3 cut(s) 71, 1303, 2534
BpuMI CCSGG 2 cut(s) 1979, 2404
BsaAI YACGTR 2 cut(s) 632, 833
BsaBI GATNNNNATC 3 cut(s) 1287, 1413, 2628
BsaHI GRCGYC 1 cut(s) 26
BsaI GGTCTC 2 cut(s) 756, 2600
BsaWI WCCGGW 2 cut(s) 146, 1743
BsaXI ACNNNNNCTCC 6 cut(s) 712, 742, 1188, 1218, 2022, 2052
Bse1I ACTGG 5 cut(s) 978, 1712, 1756, 1914, 2397
Bse3DI GCAATG 3 cut(s) 184, 195, 1772
Bse8I GATNNNNATC 3 cut(s) 1287, 1413, 2628
BseBI CCWGG 7 cut(s) 741, 895, 908, 1377, 2086, 2097, 2436
BseJI GATNNNNATC 3 cut(s) 1287, 1413, 2628
BseMI GCAATG 3 cut(s) 184, 195, 1772
BseMII CTCAG 3 cut(s) 1521, 1640, 1936
BseNI ACTGG 5 cut(s) 978, 1712, 1756, 1914, 2397
BseRI GAGGAG 1 cut(s) 1741
BseXI GCAGC 3 cut(s) 2005, 2041, 2087
BsgI GTGCAG 2 cut(s) 413, 1793
Bsh1285I CGRYCG 1 cut(s) 33
BsiEI CGRYCG 1 cut(s) 33
BsiHKAI GWGCWC 3 cut(s) 1011, 1880, 1986
BsiHKCI CYCGRG 1 cut(s) 1055
BsiSI CCGG 4 cut(s) 147, 1744, 1979, 2403
BsiWI CGTACG 1 cut(s) 632
BslFI GGGAC 3 cut(s) 31, 282, 876
BsmAI GTCTC 5 cut(s) 89, 756, 2442, 2510, 2600
BsmFI GGGAC 3 cut(s) 31, 282, 876
Bso31I GGTCTC 2 cut(s) 756, 2600
BsoBI CYCGRG 1 cut(s) 1055
Bsp119I TTCGAA 2 cut(s) 1252, 1692
Bsp1286I GDGCHC 4 cut(s) 1011, 1880, 1901, 1986
Bsp1407I TGTACA 1 cut(s) 2491
BspCNI CTCAG 3 cut(s) 1522, 1639, 1935
BspLI GGNNCC 8 cut(s) 19, 270, 495, 774, 1224, 1410, 1558, 2276
BspMI ACCTGC 1 cut(s) 1857
BspT104I TTCGAA 2 cut(s) 1252, 1692
BspTI CTTAAG 1 cut(s) 1394
BspTNI GGTCTC 2 cut(s) 756, 2600
BsrBI CCGCTC 1 cut(s) 2236
BsrDI GCAATG 3 cut(s) 184, 195, 1772
BsrGI TGTACA 1 cut(s) 2491
BsrI ACTGG 5 cut(s) 978, 1712, 1756, 1914, 2397
BssNI GRCGYC 1 cut(s) 26
BssT1I CCWWGG 3 cut(s) 1901, 1967, 2279
Bst2UI CCWGG 7 cut(s) 741, 895, 908, 1377, 2086, 2097, 2436
Bst4CI ACNGT 9 cut(s) 230, 1136, 1176, 1204, 1270, 1428, 1669, 1741, 2182
Bst6I CTCTTC 3 cut(s) 35, 1614, 2511
BstACI GRCGYC 1 cut(s) 26
BstAFI CTTAAG 1 cut(s) 1394
BstAPI GCANNNNNTGC 6 cut(s) 1771, 1850, 2063, 2115, 2172, 2249
BstAUI TGTACA 1 cut(s) 2491
BstBAI YACGTR 2 cut(s) 632, 833
BstBI TTCGAA 2 cut(s) 1252, 1692
BstC8I GCNNGC 6 cut(s) 13, 176, 314, 1191, 1809, 2264
BstDEI CTNAG 5 cut(s) 971, 1530, 1626, 1830, 1922
BstEII GGTNACC 1 cut(s) 1370
BstENI CCTNNNNNAGG 1 cut(s) 852
BstHHI GCGC 1 cut(s) 1844
BstMAI GTCTC 5 cut(s) 89, 756, 2442, 2510, 2600
BstMCI CGRYCG 1 cut(s) 33
BstNI CCWGG 7 cut(s) 741, 895, 908, 1377, 2086, 2097, 2436
BstNSI RCATGY 5 cut(s) 528, 830, 2160, 2166, 2585
BstPI GGTNACC 1 cut(s) 1370
BstSCI CCNGG 9 cut(s) 739, 893, 906, 1375, 1977, 2084, 2095, 2402, 2434
BstSFI CTRYAG 1 cut(s) 1443
BstV1I GCAGC 3 cut(s) 2005, 2041, 2087
BstX2I RGATCY 4 cut(s) 1222, 1408, 1556, 1681
BstXI CCANNNNNNTGG 1 cut(s) 799
BstYI RGATCY 4 cut(s) 1222, 1408, 1556, 1681
BsuI GTATCC 1 cut(s) 1276
BtsI GCAGTG 2 cut(s) 1781, 2041
BtsIMutI CAGTG 6 cut(s) 985, 1424, 1665, 1719, 1781, 2041
BveI ACCTGC 1 cut(s) 1857
Cac8I GCNNGC 6 cut(s) 13, 176, 314, 1191, 1809, 2264
CaiI CAGNNNCTG 2 cut(s) 1119, 2054
CfoI GCGC 1 cut(s) 1844
Cfr13I GGNCC 9 cut(s) 18, 269, 772, 784, 891, 1060, 1310, 2274, 2462
CseI GACGC 1 cut(s) 167
Csp6I GTAC 3 cut(s) 633, 2254, 2492
CviQI GTAC 3 cut(s) 633, 2254, 2492
DdeI CTNAG 5 cut(s) 971, 1530, 1626, 1830, 1922
DrdI GACNNNNNNGTC 1 cut(s) 25
DriI GACNNNNNGTC 1 cut(s) 1212
DseDI GACNNNNNNGTC 1 cut(s) 25
EaeI YGGCCR 3 cut(s) 1121, 2010, 2040
Eam1104I CTCTTC 3 cut(s) 35, 1614, 2511
Eam1105I GACNNNNNGTC 1 cut(s) 1212
EarI CTCTTC 3 cut(s) 35, 1614, 2511
EciI GGCGGA 1 cut(s) 81
Eco130I CCWWGG 3 cut(s) 1901, 1967, 2279
Eco24I GRGCYC 1 cut(s) 1901
Eco31I GGTCTC 2 cut(s) 756, 2600
Eco32I GATATC 2 cut(s) 2484, 2553
Eco47I GGWCC 3 cut(s) 18, 269, 2274
Eco57I CTGAAG 6 cut(s) 585, 1047, 1248, 1370, 1625, 2322
Eco72I CACGTG 1 cut(s) 833
Eco88I CYCGRG 1 cut(s) 1055
Eco91I GGTNACC 1 cut(s) 1370
EcoNI CCTNNNNNAGG 1 cut(s) 852
EcoO109I RGGNCCY 4 cut(s) 18, 269, 772, 1060
EcoO65I GGTNACC 1 cut(s) 1370
EcoRI GAATTC 1 cut(s) 2425
EcoRII CCWGG 7 cut(s) 739, 893, 906, 1375, 2084, 2095, 2434
EcoRV GATATC 2 cut(s) 2484, 2553
EcoT14I CCWWGG 3 cut(s) 1901, 1967, 2279
EcoT22I ATGCAT 2 cut(s) 197, 2111
EcoT38I GRGCYC 1 cut(s) 1901
ErhI CCWWGG 3 cut(s) 1901, 1967, 2279
FalI AAGNNNNNCTT 6 cut(s) 581, 613, 1312, 1344, 1855, 1887
FaqI GGGAC 3 cut(s) 31, 282, 876
FauI CCCGC 4 cut(s) 8, 890, 2229, 2645
FbaI TGATCA 1 cut(s) 2208
FblI GTMKAC 2 cut(s) 181, 2298
Fnu4HI GCNGC 8 cut(s) 9, 172, 332, 1959, 2019, 2055, 2101, 2598
FriOI GRGCYC 1 cut(s) 1901
Fsp4HI GCNGC 8 cut(s) 9, 172, 332, 1959, 2019, 2055, 2101, 2598
FspI TGCGCA 1 cut(s) 1843
GlaI GCGC 1 cut(s) 1843
GluI GCNGC 8 cut(s) 9, 172, 332, 1959, 2019, 2055, 2101, 2598
GsuI CTGGAG 5 cut(s) 948, 1215, 1632, 1695, 1706
HapII CCGG 4 cut(s) 147, 1744, 1979, 2403
HgaI GACGC 1 cut(s) 167
HhaI GCGC 1 cut(s) 1844
Hin1I GRCGYC 1 cut(s) 26
Hin6I GCGC 1 cut(s) 1842
HinP1I GCGC 1 cut(s) 1842
HincII GTYRAC 1 cut(s) 1749
HindII GTYRAC 1 cut(s) 1749
HindIII AAGCTT 6 cut(s) 386, 567, 582, 1017, 1607, 1989
HpaII CCGG 4 cut(s) 147, 1744, 1979, 2403
HphI GGTGA 5 cut(s) 266, 521, 651, 1466, 2669
Hpy166II GTNNAC 7 cut(s) 182, 659, 1335, 1494, 1643, 1749, 2299
Hpy188I TCNGA 9 cut(s) 447, 1115, 1305, 1925, 2030, 2233, 2341, 2371, 2557
Hpy8I GTNNAC 7 cut(s) 182, 659, 1335, 1494, 1643, 1749, 2299
Hpy99I CGWCG 2 cut(s) 28, 31
HpyCH4III ACNGT 9 cut(s) 230, 1136, 1176, 1204, 1270, 1428, 1669, 1741, 2182
HpyCH4IV ACGT 6 cut(s) 26, 206, 631, 832, 2169, 2328
HpyF3I CTNAG 5 cut(s) 971, 1530, 1626, 1830, 1922
HpySE526I ACGT 6 cut(s) 26, 206, 631, 832, 2169, 2328
Hsp92I GRCGYC 1 cut(s) 26
HspAI GCGC 1 cut(s) 1842
Ksp22I TGATCA 1 cut(s) 2208
LmnI GCTCC 3 cut(s) 743, 1014, 1981
Lsp1109I GCAGC 3 cut(s) 2005, 2041, 2087
MaeII ACGT 6 cut(s) 26, 206, 631, 832, 2169, 2328
MaeIII GTNAC 5 cut(s) 207, 1159, 1204, 1370, 1389
MbiI CCGCTC 1 cut(s) 2236
MboII GAAGA 9 cut(s) 52, 212, 1376, 1631, 1706, 1715, 1812, 2230, 2498
MfeI CAATTG 2 cut(s) 356, 945
MflI RGATCY 4 cut(s) 1222, 1408, 1556, 1681
MhlI GDGCHC 4 cut(s) 1011, 1880, 1901, 1986
MlsI TGGCCA 2 cut(s) 2012, 2042
MluNI TGGCCA 2 cut(s) 2012, 2042
MmeI TCCRAC 1 cut(s) 131
Mox20I TGGCCA 2 cut(s) 2012, 2042
Mph1103I ATGCAT 2 cut(s) 197, 2111
MroXI GAANNNNTTC 2 cut(s) 624, 1917
MscI TGGCCA 2 cut(s) 2012, 2042
MseI TTAA 4 cut(s) 467, 1070, 1395, 1523
MslI CAYNNNNRTG 7 cut(s) 138, 831, 1320, 1419, 1670, 1831, 2161
Msp20I TGGCCA 2 cut(s) 2012, 2042
MspA1I CMGCKG 2 cut(s) 563, 1811
MspCI CTTAAG 1 cut(s) 1394
MspI CCGG 4 cut(s) 147, 1744, 1979, 2403
MspR9I CCNGG 9 cut(s) 741, 895, 908, 1377, 1979, 2086, 2097, 2404, 2436
MunI CAATTG 2 cut(s) 356, 945
MvaI CCWGG 7 cut(s) 741, 895, 908, 1377, 2086, 2097, 2436
NciI CCSGG 2 cut(s) 1979, 2404
NlaIV GGNNCC 8 cut(s) 19, 270, 495, 774, 1224, 1410, 1558, 2276
NmeAIII GCCGAG 1 cut(s) 2465
NmuCI GTSAC 2 cut(s) 1204, 1389
NsbI TGCGCA 1 cut(s) 1843
NsiI ATGCAT 2 cut(s) 197, 2111
NspI RCATGY 5 cut(s) 528, 830, 2160, 2166, 2585
NspV TTCGAA 2 cut(s) 1252, 1692
OliI CACNNNNGTG 2 cut(s) 1320, 1670
PciI ACATGT 2 cut(s) 524, 2581
PcsI WCGNNNNNNNCGW 1 cut(s) 997
PdmI GAANNNNTTC 2 cut(s) 624, 1917
Pfl23II CGTACG 1 cut(s) 632
PflMI CCANNNNNTGG 2 cut(s) 503, 1718
PkrI GCNGC 8 cut(s) 10, 173, 333, 1960, 2020, 2056, 2102, 2599
Ple19I CGATCG 1 cut(s) 33
PmaCI CACGTG 1 cut(s) 833
PmlI CACGTG 1 cut(s) 833
Ppu21I YACGTR 2 cut(s) 632, 833
PpuMI RGGWCCY 2 cut(s) 18, 269
PscI ACATGT 2 cut(s) 524, 2581
PshBI ATTAAT 1 cut(s) 1523
Psp5II RGGWCCY 2 cut(s) 18, 269
Psp6I CCWGG 7 cut(s) 739, 893, 906, 1375, 2084, 2095, 2434
PspCI CACGTG 1 cut(s) 833
PspEI GGTNACC 1 cut(s) 1370
PspGI CCWGG 7 cut(s) 739, 893, 906, 1375, 2084, 2095, 2434
PspLI CGTACG 1 cut(s) 632
PspN4I GGNNCC 8 cut(s) 19, 270, 495, 774, 1224, 1410, 1558, 2276
PspPI GGNCC 9 cut(s) 18, 269, 772, 784, 891, 1060, 1310, 2274, 2462
PspPPI RGGWCCY 2 cut(s) 18, 269
PstNI CAGNNNCTG 2 cut(s) 1119, 2054
PsuI RGATCY 4 cut(s) 1222, 1408, 1556, 1681
PvuI CGATCG 1 cut(s) 33
PvuII CAGCTG 2 cut(s) 563, 1811
RsaI GTAC 3 cut(s) 634, 2255, 2493
RsaNI GTAC 3 cut(s) 633, 2254, 2492
RseI CAYNNNNRTG 7 cut(s) 138, 831, 1320, 1419, 1670, 1831, 2161
SaqAI TTAA 4 cut(s) 467, 1070, 1395, 1523
SatI GCNGC 8 cut(s) 9, 172, 332, 1959, 2019, 2055, 2101, 2598
Sau96I GGNCC 9 cut(s) 18, 269, 772, 784, 891, 1060, 1310, 2274, 2462
ScrFI CCNGG 9 cut(s) 741, 895, 908, 1377, 1979, 2086, 2097, 2404, 2436
SduI GDGCHC 4 cut(s) 1011, 1880, 1901, 1986
SfcI CTRYAG 1 cut(s) 1443
SfuI TTCGAA 2 cut(s) 1252, 1692
SinI GGWCC 3 cut(s) 18, 269, 2274
SmiMI CAYNNNNRTG 7 cut(s) 138, 831, 1320, 1419, 1670, 1831, 2161
SmlI CTYRAG 4 cut(s) 86, 1318, 1394, 2513
SmoI CTYRAG 4 cut(s) 86, 1318, 1394, 2513
SspI AATATT 1 cut(s) 343
StyD4I CCNGG 9 cut(s) 739, 893, 906, 1375, 1977, 2084, 2095, 2402, 2434
StyI CCWWGG 3 cut(s) 1901, 1967, 2279
TaaI ACNGT 9 cut(s) 230, 1136, 1176, 1204, 1270, 1428, 1669, 1741, 2182
TaiI ACGT 6 cut(s) 29, 209, 634, 835, 2172, 2331
TaqII GACCGA 1 cut(s) 2624
TatI WGTACW 1 cut(s) 2491
TauI GCSGC 4 cut(s) 174, 334, 1961, 2600
Tru1I TTAA 4 cut(s) 467, 1070, 1395, 1523
Tru9I TTAA 4 cut(s) 467, 1070, 1395, 1523
TscAI CASTG 6 cut(s) 985, 1431, 1672, 1719, 1781, 2041
TseFI GTSAC 2 cut(s) 1204, 1389
TseI GCWGC 4 cut(s) 8, 2018, 2054, 2100
Tsp45I GTSAC 2 cut(s) 1204, 1389
TspGWI ACGGA 1 cut(s) 880
TspRI CASTG 6 cut(s) 985, 1431, 1672, 1719, 1781, 2041
Van91I CCANNNNNTGG 2 cut(s) 503, 1718
Vha464I CTTAAG 1 cut(s) 1394
VpaK11BI GGWCC 3 cut(s) 18, 269, 2274
VspI ATTAAT 1 cut(s) 1523
XagI CCTNNNNNAGG 1 cut(s) 852
XapI RAATTY 6 cut(s) 535, 637, 808, 937, 1915, 2425
XbaI TCTAGA 1 cut(s) 1597
XceI RCATGY 5 cut(s) 528, 830, 2160, 2166, 2585
XcmI CCANNNNNNNNNTGG 1 cut(s) 2129
XmaJI CCTAGG 2 cut(s) 1967, 2279
XmiI GTMKAC 2 cut(s) 181, 2298
XmnI GAANNNNTTC 2 cut(s) 624, 1917
ZraI GACGTC 1 cut(s) 27
Zsp2I ATGCAT 2 cut(s) 197, 2111
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.