pycom04g15400

B3 domain-containing

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0001663
Physical Location & Seq
Reverse (-)
18299696 .. 18302672
2977 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g15400.5

Sequence Viewer

Length: 1308 bp
ATGAACGCAGCTTGCGGGACCTCGACGTCGATCGAGTGGAAGAGAGGTTGGGTTTTGCGATCTGGCGGATTCGCCAATCTCTGCATCAAGTGCTGGTCTGTATATGAGCAATCGATTTATTGTGATGTATACCACTCAGAGGAATCTGGTTGGAGGGAATGCGGAGTATGTGGCAAGCGTCTACATTGCGGATGCATTGCTTCCACGTTACTGCTCGATCTTCTTGATGGTGGTGGTGTGAAGTGCATAAAATGTGCAAAGGATTCGGGACCTCACCCTATTTCAAGTGATGAAAAGCCTGATGGTCTTGGAACATCCAAAATCAGTGAACCGCAATCAAATATTACAGACAATCAATTGGATGGACGTGATGTTGAAAAGTTGAAGCTTGTGCAGTTGGGAAATAATAAAGATTCTAATGGACTTATGAACTTGCTTCAACTTCGGAACGACAACACAAATGGGCTAATGCTCAAATTGAAACATGATGATGTTCCACCTCCTGGGGGTGAAATTGGAGGCGCATGTTTTTCAAATTTTAATCAGGCACCCCATGGATCATCTGAAGCTTCCAAAGCAGAAGTTTTCAAGGCAAACTTAGGGATAAATAATTTATATGAATCACTTCCACATACAAATTTGAGTATGACCCTCGGTTCACCTTTGGGAAAGGCATATCCTTTTCCTGGTGCCGTTGTTGATGAAAGGGAACACAGCAAGACACCCTCTCCACTCCCTCTAGGAGCTAGGCCTCAACATCTTTTTCCAAAGCCCCCAAAATTGGCCCTTTCCACAGGTTTGGAGGAAAAATCTACAATGGTTTCACATGTACGTGTGGCAAGGCCACCTGCCGAAGGACGGGGACGGAATCAGCTACTTCCCCGCTATTGGCCCAGGATTACAGACCAGGAATTACAGCAAATATCTGGAGATTCAAATTCCACAATTGTTCCCTTGTTTGAAAAGATGCTAAGTGCCAGTGATGCTGGTCGAATTGGTCGTTTGGTGCTTCCTAAAGCTTGTGCTGAGGCATATTTTCCTCCCATCTCTCAACCAGAGGGGCTTCCGTTAAGGATTCAAGATGTGAAGGGAAAAGAATGGATGTTTCAGTTCAGATTCTGGCCGAATAACAACAGTAGGATGTATGTTTTGGAGGGTGTAACCCCCTGCATACAGTCCATGCAGTTGCAGGCTGGAGATACTGTGACTTTCAGTCGTATGGATCCTGAAGGAAAACTAATTATGGGGTTTCGGAAAGCATCGAATACTGTTGCAATTCATTTAAGGGAAGGATGGATCCCCATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3 zf-CW

Protein Analysis

436

Amino Acids

47.72

Weight (kDa)

8.16

Isoelectric Point (pI)

42.49

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 856
AasI GACNNNNNNGTC 1 cut(s) 25
AatII GACGTC 1 cut(s) 29
Acc36I ACCTGC 1 cut(s) 856
AccB1I GGYRCC 2 cut(s) 547, 689
AccB7I CCANNNNNTGG 1 cut(s) 503
AccI GTMKAC 2 cut(s) 129, 181
AciI CCGC 6 cut(s) 15, 66, 162, 189, 332, 883
AclWI GGATC 5 cut(s) 565, 1217, 1230, 1291, 1304
AcoI YGGCCR 1 cut(s) 1121
AcsI RAATTY 3 cut(s) 535, 637, 937
AcuI CTGAAG 2 cut(s) 585, 1248
AcyI GRCGYC 1 cut(s) 26
AfaI GTAC 1 cut(s) 831
AfiI CCNNNNNNNGG 8 cut(s) 139, 503, 506, 686, 781, 854, 858, 888
AflIII ACRYGT 2 cut(s) 826, 832
AhdI GACNNNNNGTC 1 cut(s) 1212
AjiI CACGTC 1 cut(s) 368
AjnI CCWGG 4 cut(s) 502, 685, 893, 906
AluBI AGCT 6 cut(s) 11, 388, 569, 746, 874, 1019
AluI AGCT 6 cut(s) 11, 388, 569, 746, 874, 1019
AlwI GGATC 5 cut(s) 565, 1217, 1230, 1291, 1304
AlwNI CAGNNNCTG 1 cut(s) 1119
AoxI GGCC 5 cut(s) 749, 783, 842, 890, 1121
ApeKI GCWGC 1 cut(s) 8
ApoI RAATTY 3 cut(s) 535, 637, 937
Asp700I GAANNNNTTC 1 cut(s) 624
AspLEI GCGC 1 cut(s) 524
AspS9I GGNCC 4 cut(s) 18, 269, 784, 891
AsuHPI GGTGA 3 cut(s) 266, 521, 651
AvaII GGWCC 2 cut(s) 18, 269
BamHI GGATCC 2 cut(s) 1222, 1296
BanI GGYRCC 2 cut(s) 547, 689
BbvCI CCTCAGC 1 cut(s) 1026
BbvI GCAGC 1 cut(s) 20
BccI CCATC 5 cut(s) 221, 296, 356, 1052, 1287
BceAI ACGGC 1 cut(s) 677
BcgI CGANNNNNNTGC 2 cut(s) 246, 280
BciT130I CCWGG 4 cut(s) 504, 687, 895, 908
BfaI CTAG 2 cut(s) 740, 747
BfuAI ACCTGC 1 cut(s) 856
BisI GCNGC 1 cut(s) 9
BlsI GCNGC 1 cut(s) 10
Bme1390I CCNGG 4 cut(s) 504, 687, 895, 908
Bme18I GGWCC 2 cut(s) 18, 269
BmeRI GACNNNNNGTC 1 cut(s) 1212
BmgBI CACGTC 1 cut(s) 368
BmgT120I GGNCC 4 cut(s) 18, 269, 784, 891
BmiI GGNNCC 6 cut(s) 19, 270, 549, 691, 1224, 1298
BmrFI CCNGG 4 cut(s) 504, 687, 895, 908
BmsI GCATC 5 cut(s) 93, 182, 957, 973, 1268
BpmI CTGGAG 2 cut(s) 948, 1215
Bpu10I CCTNAGC 1 cut(s) 1026
Bsa29I ATCGAT 1 cut(s) 113
BsaAI YACGTR 1 cut(s) 833
BsaBI GATNNNNATC 1 cut(s) 1301
BsaHI GRCGYC 1 cut(s) 26
BsaJI CCNNGG 4 cut(s) 503, 553, 652, 893
BsaXI ACNNNNNCTCC 4 cut(s) 712, 742, 1188, 1218
Bsc4I CCNNNNNNNGG 8 cut(s) 139, 503, 506, 686, 781, 854, 858, 888
Bse1I ACTGG 1 cut(s) 978
Bse3DI GCAATG 2 cut(s) 184, 195
Bse8I GATNNNNATC 1 cut(s) 1301
BseBI CCWGG 4 cut(s) 504, 687, 895, 908
BseCI ATCGAT 1 cut(s) 113
BseDI CCNNGG 4 cut(s) 503, 553, 652, 893
BseGI GGATG 6 cut(s) 197, 314, 367, 1107, 1146, 1298
BseJI GATNNNNATC 1 cut(s) 1301
BseLI CCNNNNNNNGG 8 cut(s) 139, 503, 506, 686, 781, 854, 858, 888
BseMI GCAATG 2 cut(s) 184, 195
BseMII CTCAG 2 cut(s) 150, 1017
BseNI ACTGG 1 cut(s) 978
BseXI GCAGC 1 cut(s) 20
BsgI GTGCAG 1 cut(s) 413
Bsh1285I CGRYCG 1 cut(s) 33
BshFI GGCC 5 cut(s) 751, 785, 844, 892, 1123
BshNI GGYRCC 2 cut(s) 547, 689
BshVI ATCGAT 1 cut(s) 113
BsiEI CGRYCG 1 cut(s) 33
BslFI GGGAC 3 cut(s) 31, 282, 876
BslI CCNNNNNNNGG 8 cut(s) 139, 503, 506, 686, 781, 854, 858, 888
BsmFI GGGAC 3 cut(s) 31, 282, 876
BsmI GAATGC 1 cut(s) 164
BsnI GGCC 5 cut(s) 751, 785, 844, 892, 1123
Bsp143I GATC 6 cut(s) 30, 59, 217, 557, 1222, 1296
Bsp19I CCATGG 1 cut(s) 553
BspACI CCGC 6 cut(s) 15, 66, 162, 189, 332, 883
BspANI GGCC 5 cut(s) 751, 785, 844, 892, 1123
BspCNI CTCAG 2 cut(s) 149, 1018
BspDI ATCGAT 1 cut(s) 113
BspLI GGNNCC 6 cut(s) 19, 270, 549, 691, 1224, 1298
BspMI ACCTGC 1 cut(s) 856
BspPI GGATC 5 cut(s) 565, 1217, 1230, 1291, 1304
BspT107I GGYRCC 2 cut(s) 547, 689
BsrDI GCAATG 2 cut(s) 184, 195
BsrI ACTGG 1 cut(s) 978
BssECI CCNNGG 4 cut(s) 503, 553, 652, 893
BssMI GATC 6 cut(s) 30, 59, 217, 557, 1222, 1296
BssNAI GTATAC 1 cut(s) 130
BssNI GRCGYC 1 cut(s) 26
BssT1I CCWWGG 1 cut(s) 553
Bst1107I GTATAC 1 cut(s) 130
Bst2UI CCWGG 4 cut(s) 504, 687, 895, 908
Bst4CI ACNGT 4 cut(s) 1136, 1176, 1204, 1270
Bst6I CTCTTC 1 cut(s) 35
BstACI GRCGYC 1 cut(s) 26
BstAPI GCANNNNNTGC 1 cut(s) 90
BstBAI YACGTR 1 cut(s) 833
BstC8I GCNNGC 3 cut(s) 13, 176, 1191
BstDEI CTNAG 4 cut(s) 136, 598, 971, 1026
BstDSI CCRYGG 1 cut(s) 553
BstENI CCTNNNNNAGG 1 cut(s) 852
BstF5I GGATG 6 cut(s) 197, 314, 367, 1107, 1146, 1298
BstHHI GCGC 1 cut(s) 524
BstKTI GATC 6 cut(s) 33, 62, 220, 560, 1225, 1299
BstMBI GATC 6 cut(s) 30, 59, 217, 557, 1222, 1296
BstMCI CGRYCG 1 cut(s) 33
BstMWI GCNNNNNNNGC 3 cut(s) 90, 575, 983
BstNI CCWGG 4 cut(s) 504, 687, 895, 908
BstNSI RCATGY 2 cut(s) 528, 830
BstSCI CCNGG 4 cut(s) 502, 685, 893, 906
BstV1I GCAGC 1 cut(s) 20
BstX2I RGATCY 2 cut(s) 1222, 1296
BstXI CCANNNNNNTGG 1 cut(s) 799
BstYI RGATCY 2 cut(s) 1222, 1296
BstZ17I GTATAC 1 cut(s) 130
Bsu15I ATCGAT 1 cut(s) 113
BsuRI GGCC 5 cut(s) 751, 785, 844, 892, 1123
BsuTUI ATCGAT 1 cut(s) 113
BtgI CCRYGG 1 cut(s) 553
BtrI CACGTC 1 cut(s) 368
BtsCI GGATG 6 cut(s) 197, 314, 367, 1107, 1146, 1298
BtsIMutI CAGTG 2 cut(s) 331, 985
BveI ACCTGC 1 cut(s) 856
Cac8I GCNNGC 3 cut(s) 13, 176, 1191
CaiI CAGNNNCTG 1 cut(s) 1119
CfoI GCGC 1 cut(s) 524
Cfr13I GGNCC 4 cut(s) 18, 269, 784, 891
ClaI ATCGAT 1 cut(s) 113
CseI GACGC 1 cut(s) 167
Csp6I GTAC 1 cut(s) 830
CviAII CATG 5 cut(s) 485, 525, 554, 827, 1180
CviQI GTAC 1 cut(s) 830
DdeI CTNAG 4 cut(s) 136, 598, 971, 1026
DpnI GATC 6 cut(s) 32, 61, 219, 559, 1224, 1298
DpnII GATC 6 cut(s) 30, 59, 217, 557, 1222, 1296
DrdI GACNNNNNNGTC 1 cut(s) 25
DriI GACNNNNNGTC 1 cut(s) 1212
DseDI GACNNNNNNGTC 1 cut(s) 25
EaeI YGGCCR 1 cut(s) 1121
Eam1104I CTCTTC 1 cut(s) 35
Eam1105I GACNNNNNGTC 1 cut(s) 1212
EarI CTCTTC 1 cut(s) 35
EciI GGCGGA 1 cut(s) 81
Eco130I CCWWGG 1 cut(s) 553
Eco147I AGGCCT 1 cut(s) 751
Eco47I GGWCC 2 cut(s) 18, 269
Eco57I CTGAAG 2 cut(s) 585, 1248
EcoNI CCTNNNNNAGG 1 cut(s) 852
EcoO109I RGGNCCY 2 cut(s) 18, 269
EcoRII CCWGG 4 cut(s) 502, 685, 893, 906
EcoT14I CCWWGG 1 cut(s) 553
EcoT22I ATGCAT 1 cut(s) 197
ErhI CCWWGG 1 cut(s) 553
FaeI CATG 5 cut(s) 488, 528, 557, 830, 1183
FalI AAGNNNNNCTT 2 cut(s) 581, 613
FaqI GGGAC 3 cut(s) 31, 282, 876
FatI CATG 5 cut(s) 484, 524, 553, 826, 1179
FauI CCCGC 2 cut(s) 8, 890
FblI GTMKAC 2 cut(s) 129, 181
Fnu4HI GCNGC 1 cut(s) 9
FokI GGATG 5 cut(s) 204, 301, 374, 1114, 1153
Fsp4HI GCNGC 1 cut(s) 9
FspBI CTAG 2 cut(s) 740, 747
GlaI GCGC 1 cut(s) 523
GluI GCNGC 1 cut(s) 9
GsuI CTGGAG 2 cut(s) 948, 1215
HaeIII GGCC 5 cut(s) 751, 785, 844, 892, 1123
HgaI GACGC 1 cut(s) 167
HhaI GCGC 1 cut(s) 524
Hin1I GRCGYC 1 cut(s) 26
Hin1II CATG 5 cut(s) 488, 528, 557, 830, 1183
Hin6I GCGC 1 cut(s) 522
HinP1I GCGC 1 cut(s) 522
HindIII AAGCTT 3 cut(s) 386, 567, 1017
HinfI GANTC 9 cut(s) 69, 143, 263, 413, 620, 868, 932, 1075, 1116
HphI GGTGA 3 cut(s) 266, 521, 651
Hpy166II GTNNAC 4 cut(s) 130, 182, 329, 659
Hpy188I TCNGA 5 cut(s) 139, 447, 565, 1115, 1254
Hpy188III TCNNGA 5 cut(s) 224, 267, 927, 1079, 1226
Hpy8I GTNNAC 4 cut(s) 130, 182, 329, 659
Hpy99I CGWCG 2 cut(s) 28, 31
HpyAV CCTTC 4 cut(s) 848, 1081, 1223, 1283
HpyCH4III ACNGT 4 cut(s) 1136, 1176, 1204, 1270
HpyCH4IV ACGT 4 cut(s) 26, 206, 367, 832
HpyCH4V TGCA 9 cut(s) 84, 195, 246, 257, 394, 1170, 1183, 1189, 1274
HpyF10VI GCNNNNNNNGC 3 cut(s) 90, 575, 983
HpyF3I CTNAG 4 cut(s) 136, 598, 971, 1026
HpySE526I ACGT 4 cut(s) 26, 206, 367, 832
Hsp92I GRCGYC 1 cut(s) 26
Hsp92II CATG 5 cut(s) 488, 528, 557, 830, 1183
HspAI GCGC 1 cut(s) 522
Kzo9I GATC 6 cut(s) 30, 59, 217, 557, 1222, 1296
LmnI GCTCC 1 cut(s) 743
Lsp1109I GCAGC 1 cut(s) 20
LweI GCATC 5 cut(s) 93, 182, 957, 973, 1268
MaeI CTAG 2 cut(s) 740, 747
MaeII ACGT 4 cut(s) 26, 206, 367, 832
MaeIII GTNAC 3 cut(s) 207, 1159, 1204
MalI GATC 6 cut(s) 32, 61, 219, 559, 1224, 1298
MboI GATC 6 cut(s) 30, 59, 217, 557, 1222, 1296
MboII GAAGA 2 cut(s) 52, 212
MfeI CAATTG 2 cut(s) 356, 945
MflI RGATCY 2 cut(s) 1222, 1296
MmeI TCCRAC 1 cut(s) 131
Mph1103I ATGCAT 1 cut(s) 197
MroXI GAANNNNTTC 1 cut(s) 624
MseI TTAA 3 cut(s) 540, 1070, 1283
MslI CAYNNNNRTG 2 cut(s) 489, 831
MspR9I CCNGG 4 cut(s) 504, 687, 895, 908
MunI CAATTG 2 cut(s) 356, 945
Mva1269I GAATGC 1 cut(s) 164
MvaI CCWGG 4 cut(s) 504, 687, 895, 908
MwoI GCNNNNNNNGC 3 cut(s) 90, 575, 983
NcoI CCATGG 1 cut(s) 553
NdeII GATC 6 cut(s) 30, 59, 217, 557, 1222, 1296
NlaIII CATG 5 cut(s) 488, 528, 557, 830, 1183
NlaIV GGNNCC 6 cut(s) 19, 270, 549, 691, 1224, 1298
NmuCI GTSAC 1 cut(s) 1204
NsiI ATGCAT 1 cut(s) 197
NspI RCATGY 2 cut(s) 528, 830
PaqCI CACCTGC 1 cut(s) 856
PceI AGGCCT 1 cut(s) 751
PciI ACATGT 1 cut(s) 826
PcsI WCGNNNNNNNCGW 1 cut(s) 997
PctI GAATGC 1 cut(s) 164
PdmI GAANNNNTTC 1 cut(s) 624
PfeI GAWTC 9 cut(s) 69, 143, 263, 413, 620, 868, 932, 1075, 1116
PflMI CCANNNNNTGG 1 cut(s) 503
PkrI GCNGC 1 cut(s) 10
Ple19I CGATCG 1 cut(s) 33
Ppu21I YACGTR 1 cut(s) 833
PpuMI RGGWCCY 2 cut(s) 18, 269
PscI ACATGT 1 cut(s) 826
Psp5II RGGWCCY 2 cut(s) 18, 269
Psp6I CCWGG 4 cut(s) 502, 685, 893, 906
PspGI CCWGG 4 cut(s) 502, 685, 893, 906
PspN4I GGNNCC 6 cut(s) 19, 270, 549, 691, 1224, 1298
PspPI GGNCC 4 cut(s) 18, 269, 784, 891
PspPPI RGGWCCY 2 cut(s) 18, 269
PstNI CAGNNNCTG 1 cut(s) 1119
PsuI RGATCY 2 cut(s) 1222, 1296
PvuI CGATCG 1 cut(s) 33
RsaI GTAC 1 cut(s) 831
RsaNI GTAC 1 cut(s) 830
RseI CAYNNNNRTG 2 cut(s) 489, 831
SaqAI TTAA 3 cut(s) 540, 1070, 1283
SatI GCNGC 1 cut(s) 9
Sau3AI GATC 6 cut(s) 30, 59, 217, 557, 1222, 1296
Sau96I GGNCC 4 cut(s) 18, 269, 784, 891
ScrFI CCNGG 4 cut(s) 504, 687, 895, 908
SfaNI GCATC 5 cut(s) 93, 182, 957, 973, 1268
SinI GGWCC 2 cut(s) 18, 269
SmiMI CAYNNNNRTG 2 cut(s) 489, 831
SseBI AGGCCT 1 cut(s) 751
SsiI CCGC 6 cut(s) 15, 66, 162, 189, 332, 883
SspI AATATT 1 cut(s) 343
SspMI CTAG 2 cut(s) 740, 747
StuI AGGCCT 1 cut(s) 751
StyD4I CCNGG 4 cut(s) 502, 685, 893, 906
StyI CCWWGG 1 cut(s) 553
TaaI ACNGT 4 cut(s) 1136, 1176, 1204, 1270
TaiI ACGT 4 cut(s) 29, 209, 370, 835
TaqI TCGA 7 cut(s) 23, 29, 33, 113, 216, 991, 1262
TfiI GAWTC 9 cut(s) 69, 143, 263, 413, 620, 868, 932, 1075, 1116
Tru1I TTAA 3 cut(s) 540, 1070, 1283
Tru9I TTAA 3 cut(s) 540, 1070, 1283
TscAI CASTG 2 cut(s) 331, 985
TseFI GTSAC 1 cut(s) 1204
TseI GCWGC 1 cut(s) 8
Tsp45I GTSAC 1 cut(s) 1204
TspDTI ATGAA 6 cut(s) 17, 306, 443, 633, 717, 1268
TspGWI ACGGA 2 cut(s) 880, 1056
TspRI CASTG 2 cut(s) 331, 985
Van91I CCANNNNNTGG 1 cut(s) 503
VpaK11BI GGWCC 2 cut(s) 18, 269
XagI CCTNNNNNAGG 1 cut(s) 852
XapI RAATTY 3 cut(s) 535, 637, 937
XceI RCATGY 2 cut(s) 528, 830
XmiI GTMKAC 2 cut(s) 129, 181
XmnI GAANNNNTTC 1 cut(s) 624
XspI CTAG 2 cut(s) 740, 747
ZraI GACGTC 1 cut(s) 27
Zsp2I ATGCAT 1 cut(s) 197
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.