B3 domain-containing
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 1593 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 114, 1500 |
| AccBSI | CCGCTC | 1 cut(s) | 2173 |
| AccI | GTMKAC | 1 cut(s) | 702 |
| AccII | CGCG | 1 cut(s) | 1020 |
| AccIII | TCCGGA | 1 cut(s) | 159 |
| AciI | CCGC | 5 cut(s) | 418, 1020, 1558, 2173, 2439 |
| AclI | AACGTT | 1 cut(s) | 806 |
| AclWI | GGATC | 2 cut(s) | 151, 1454 |
| AcoI | YGGCCR | 2 cut(s) | 651, 1491 |
| AcsI | RAATTY | 7 cut(s) | 776, 938, 995, 1033, 1363, 1810, 2537 |
| AcuI | CTGAAG | 4 cut(s) | 164, 486, 527, 1763 |
| AfaI | GTAC | 8 cut(s) | 65, 288, 369, 515, 893, 1959, 2250, 2650 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 114, 1426, 1500 |
| AjnI | CCWGG | 2 cut(s) | 126, 2498 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 344, 376, 1516, 1548 |
| Alw21I | GWGCWC | 1 cut(s) | 843 |
| Alw26I | GTCTC | 3 cut(s) | 62, 1450, 2170 |
| AlwI | GGATC | 2 cut(s) | 151, 1454 |
| Aor13HI | TCCGGA | 1 cut(s) | 159 |
| AoxI | GGCC | 5 cut(s) | 651, 1083, 1142, 1491, 1662 |
| ApaI | GGGCCC | 1 cut(s) | 1666 |
| ApeKI | GCWGC | 2 cut(s) | 1787, 1998 |
| ApoI | RAATTY | 7 cut(s) | 776, 938, 995, 1033, 1363, 1810, 2537 |
| ArsI | GACNNNNNNTTYG | 4 cut(s) | 209, 239, 241, 271 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 177, 1267 |
| AspS9I | GGNCC | 4 cut(s) | 924, 1142, 1662, 1663 |
| AsuC2I | CCSGG | 1 cut(s) | 148 |
| AsuHPI | GGTGA | 4 cut(s) | 97, 1440, 2294, 2501 |
| AsuII | TTCGAA | 1 cut(s) | 171 |
| AvaII | GGWCC | 1 cut(s) | 924 |
| AxyI | CCTNAGG | 1 cut(s) | 2127 |
| BaeGI | GKGCMC | 1 cut(s) | 1666 |
| BaeI | ACNNNNGTAYC | 3 cut(s) | 674, 707, 2640 |
| BalI | TGGCCA | 1 cut(s) | 1493 |
| BanII | GRGCYC | 2 cut(s) | 843, 1666 |
| BbsI | GAAGAC | 2 cut(s) | 81, 1607 |
| Bbv12I | GWGCWC | 1 cut(s) | 843 |
| BbvI | GCAGC | 2 cut(s) | 1774, 1985 |
| BccI | CCATC | 3 cut(s) | 226, 554, 1214 |
| BceAI | ACGGC | 1 cut(s) | 2639 |
| BciT130I | CCWGG | 2 cut(s) | 128, 2500 |
| BciVI | GTATCC | 1 cut(s) | 2624 |
| BclI | TGATCA | 2 cut(s) | 1195, 2506 |
| BcnI | CCSGG | 1 cut(s) | 148 |
| BcoDI | GTCTC | 3 cut(s) | 62, 1450, 2170 |
| BfaI | CTAG | 2 cut(s) | 53, 548 |
| BfmI | CTRYAG | 1 cut(s) | 1999 |
| BfuI | GTATCC | 1 cut(s) | 2624 |
| BglII | AGATCT | 2 cut(s) | 1284, 2068 |
| BisI | GCNGC | 4 cut(s) | 1559, 1788, 1999, 2439 |
| BlpI | GCTNAGC | 1 cut(s) | 1632 |
| BlsI | GCNGC | 4 cut(s) | 1560, 1789, 2000, 2440 |
| Bme1390I | CCNGG | 3 cut(s) | 128, 148, 2500 |
| Bme18I | GGWCC | 1 cut(s) | 924 |
| BmgT120I | GGNCC | 4 cut(s) | 924, 1142, 1662, 1663 |
| BmiI | GGNNCC | 1 cut(s) | 1664 |
| BmrFI | CCNGG | 3 cut(s) | 128, 148, 2500 |
| BmrI | ACTGGG | 1 cut(s) | 1949 |
| BmsI | GCATC | 5 cut(s) | 23, 514, 1327, 1425, 1792 |
| BmuI | ACTGGG | 1 cut(s) | 1949 |
| BpiI | GAAGAC | 2 cut(s) | 81, 1607 |
| BpmI | CTGGAG | 3 cut(s) | 110, 1143, 1722 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1632 |
| Bpu14I | TTCGAA | 1 cut(s) | 171 |
| BpuEI | CTTGAG | 4 cut(s) | 770, 1113, 1115, 2129 |
| BpuMI | CCSGG | 1 cut(s) | 148 |
| Bsa29I | ATCGAT | 1 cut(s) | 314 |
| BsaI | GGTCTC | 1 cut(s) | 62 |
| BsaJI | CCNNGG | 5 cut(s) | 207, 1044, 2403, 2498, 2635 |
| BsaWI | WCCGGW | 1 cut(s) | 159 |
| BsaXI | ACNNNNNCTCC | 6 cut(s) | 383, 413, 908, 938, 1720, 1750 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 114, 1426, 1500 |
| Bse1I | ACTGG | 3 cut(s) | 1494, 1739, 1955 |
| Bse21I | CCTNAGG | 1 cut(s) | 2127 |
| Bse3DI | GCAATG | 2 cut(s) | 1347, 1488 |
| BseAI | TCCGGA | 1 cut(s) | 159 |
| BseBI | CCWGG | 2 cut(s) | 128, 2500 |
| BseCI | ATCGAT | 1 cut(s) | 314 |
| BseDI | CCNNGG | 5 cut(s) | 207, 1044, 2403, 2498, 2635 |
| BseGI | GGATG | 7 cut(s) | 14, 529, 833, 1289, 1342, 1416, 2038 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 114, 1426, 1500 |
| BseMI | GCAATG | 2 cut(s) | 1347, 1488 |
| BseMII | CTCAG | 4 cut(s) | 434, 1646, 2118, 2310 |
| BseNI | ACTGG | 3 cut(s) | 1494, 1739, 1955 |
| BseRI | GAGGAG | 8 cut(s) | 347, 617, 680, 869, 932, 1033, 1613, 1616 |
| BseSI | GKGCMC | 1 cut(s) | 1666 |
| BseXI | GCAGC | 2 cut(s) | 1774, 1985 |
| BseYI | CCCAGC | 1 cut(s) | 1816 |
| Bsh1236I | CGCG | 1 cut(s) | 1020 |
| Bsh1285I | CGRYCG | 1 cut(s) | 692 |
| BshFI | GGCC | 5 cut(s) | 653, 1085, 1144, 1493, 1664 |
| BshVI | ATCGAT | 1 cut(s) | 314 |
| BsiEI | CGRYCG | 1 cut(s) | 692 |
| BsiHKAI | GWGCWC | 1 cut(s) | 843 |
| BsiSI | CCGG | 2 cut(s) | 148, 160 |
| BsiWI | CGTACG | 1 cut(s) | 63 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 114, 1426, 1500 |
| BsmAI | GTCTC | 3 cut(s) | 62, 1450, 2170 |
| BsmBI | CGTCTC | 1 cut(s) | 2170 |
| BsmI | GAATGC | 1 cut(s) | 157 |
| BsnI | GGCC | 5 cut(s) | 653, 1085, 1144, 1493, 1664 |
| Bso31I | GGTCTC | 1 cut(s) | 62 |
| Bsp119I | TTCGAA | 1 cut(s) | 171 |
| Bsp120I | GGGCCC | 1 cut(s) | 1662 |
| Bsp1286I | GDGCHC | 2 cut(s) | 843, 1666 |
| Bsp13I | TCCGGA | 1 cut(s) | 159 |
| Bsp143I | GATC | 9 cut(s) | 156, 311, 408, 1195, 1284, 1459, 1879, 2068, 2506 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1632 |
| BspACI | CCGC | 5 cut(s) | 418, 1020, 1558, 2173, 2439 |
| BspANI | GGCC | 5 cut(s) | 653, 1085, 1144, 1493, 1664 |
| BspCNI | CTCAG | 4 cut(s) | 435, 1645, 2119, 2311 |
| BspDI | ATCGAT | 1 cut(s) | 314 |
| BspEI | TCCGGA | 1 cut(s) | 159 |
| BspFNI | CGCG | 1 cut(s) | 1020 |
| BspLI | GGNNCC | 1 cut(s) | 1664 |
| BspMAI | CTGCAG | 1 cut(s) | 2003 |
| BspPI | GGATC | 2 cut(s) | 151, 1454 |
| BspQI | GCTCTTC | 2 cut(s) | 168, 786 |
| BspT104I | TTCGAA | 1 cut(s) | 171 |
| BspTNI | GGTCTC | 1 cut(s) | 62 |
| BsrBI | CCGCTC | 1 cut(s) | 2173 |
| BsrDI | GCAATG | 2 cut(s) | 1347, 1488 |
| BsrI | ACTGG | 3 cut(s) | 1494, 1739, 1955 |
| BssECI | CCNNGG | 5 cut(s) | 207, 1044, 2403, 2498, 2635 |
| BssMI | GATC | 9 cut(s) | 156, 311, 408, 1195, 1284, 1459, 1879, 2068, 2506 |
| BssNAI | GTATAC | 1 cut(s) | 703 |
| BssT1I | CCWWGG | 2 cut(s) | 207, 2403 |
| Bst1107I | GTATAC | 1 cut(s) | 703 |
| Bst2UI | CCWGG | 2 cut(s) | 128, 2500 |
| Bst4CI | ACNGT | 8 cut(s) | 135, 690, 707, 896, 1372, 2588, 2636, 2643 |
| Bst6I | CTCTTC | 8 cut(s) | 168, 342, 347, 462, 620, 786, 1253, 1771 |
| BstBI | TTCGAA | 1 cut(s) | 171 |
| BstC8I | GCNNGC | 3 cut(s) | 432, 932, 1785 |
| BstDSI | CCRYGG | 1 cut(s) | 2635 |
| BstF5I | GGATG | 7 cut(s) | 14, 529, 833, 1289, 1342, 1416, 2038 |
| BstFNI | CGCG | 1 cut(s) | 1020 |
| BstKTI | GATC | 9 cut(s) | 159, 314, 411, 1198, 1287, 1462, 1882, 2071, 2509 |
| BstMAI | GTCTC | 3 cut(s) | 62, 1450, 2170 |
| BstMBI | GATC | 9 cut(s) | 156, 311, 408, 1195, 1284, 1459, 1879, 2068, 2506 |
| BstMCI | CGRYCG | 1 cut(s) | 692 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 1169, 2230, 2389 |
| BstNI | CCWGG | 2 cut(s) | 128, 2500 |
| BstNSI | RCATGY | 2 cut(s) | 1416, 1691 |
| BstSCI | CCNGG | 3 cut(s) | 126, 146, 2498 |
| BstSFI | CTRYAG | 1 cut(s) | 1999 |
| BstSLI | GKGCMC | 1 cut(s) | 1666 |
| BstUI | CGCG | 1 cut(s) | 1020 |
| BstV1I | GCAGC | 2 cut(s) | 1774, 1985 |
| BstV2I | GAAGAC | 2 cut(s) | 81, 1607 |
| BstX2I | RGATCY | 2 cut(s) | 1284, 2068 |
| BstYI | RGATCY | 2 cut(s) | 1284, 2068 |
| BstZ17I | GTATAC | 1 cut(s) | 703 |
| Bsu15I | ATCGAT | 1 cut(s) | 314 |
| Bsu36I | CCTNAGG | 1 cut(s) | 2127 |
| BsuI | GTATCC | 1 cut(s) | 2624 |
| BsuRI | GGCC | 5 cut(s) | 653, 1085, 1144, 1493, 1664 |
| BsuTUI | ATCGAT | 1 cut(s) | 314 |
| BtgI | CCRYGG | 1 cut(s) | 2635 |
| BtsCI | GGATG | 7 cut(s) | 14, 529, 833, 1289, 1342, 1416, 2038 |
| BtsI | GCAGTG | 2 cut(s) | 2525, 2531 |
| BtsIMutI | CAGTG | 4 cut(s) | 875, 1501, 2525, 2531 |
| Cac8I | GCNNGC | 3 cut(s) | 432, 932, 1785 |
| Cfr13I | GGNCC | 4 cut(s) | 924, 1142, 1662, 1663 |
| ClaI | ATCGAT | 1 cut(s) | 314 |
| CseI | GACGC | 1 cut(s) | 75 |
| Csp6I | GTAC | 8 cut(s) | 64, 287, 368, 514, 892, 1958, 2249, 2649 |
| CspCI | CAANNNNNGTGG | 4 cut(s) | 1780, 1815, 2501, 2536 |
| CviAII | CATG | 6 cut(s) | 518, 1413, 1637, 1688, 1930, 2618 |
| CviQI | GTAC | 8 cut(s) | 64, 287, 368, 514, 892, 1958, 2249, 2649 |
| DpnI | GATC | 9 cut(s) | 158, 313, 410, 1197, 1286, 1461, 1881, 2070, 2508 |
| DpnII | GATC | 9 cut(s) | 156, 311, 408, 1195, 1284, 1459, 1879, 2068, 2506 |
| DraI | TTTAAA | 2 cut(s) | 943, 1000 |
| EaeI | YGGCCR | 2 cut(s) | 651, 1491 |
| Eam1104I | CTCTTC | 8 cut(s) | 168, 342, 347, 462, 620, 786, 1253, 1771 |
| EarI | CTCTTC | 8 cut(s) | 168, 342, 347, 462, 620, 786, 1253, 1771 |
| EciI | GGCGGA | 1 cut(s) | 433 |
| Ecl136II | GAGCTC | 1 cut(s) | 841 |
| Eco130I | CCWWGG | 2 cut(s) | 207, 2403 |
| Eco24I | GRGCYC | 2 cut(s) | 843, 1666 |
| Eco31I | GGTCTC | 1 cut(s) | 62 |
| Eco47I | GGWCC | 1 cut(s) | 924 |
| Eco53kI | GAGCTC | 1 cut(s) | 841 |
| Eco57I | CTGAAG | 4 cut(s) | 164, 486, 527, 1763 |
| Eco81I | CCTNAGG | 1 cut(s) | 2127 |
| EcoICRI | GAGCTC | 1 cut(s) | 841 |
| EcoO109I | RGGNCCY | 1 cut(s) | 1663 |
| EcoRI | GAATTC | 1 cut(s) | 1033 |
| EcoRII | CCWGG | 2 cut(s) | 126, 2498 |
| EcoT14I | CCWWGG | 2 cut(s) | 207, 2403 |
| EcoT22I | ATGCAT | 1 cut(s) | 1418 |
| EcoT38I | GRGCYC | 2 cut(s) | 843, 1666 |
| ErhI | CCWWGG | 2 cut(s) | 207, 2403 |
| Esp3I | CGTCTC | 1 cut(s) | 2170 |
| FaeI | CATG | 6 cut(s) | 521, 1416, 1640, 1691, 1933, 2621 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 344, 376 |
| FatI | CATG | 6 cut(s) | 517, 1412, 1636, 1687, 1929, 2617 |
| FauNDI | CATATG | 1 cut(s) | 1254 |
| FbaI | TGATCA | 2 cut(s) | 1195, 2506 |
| FblI | GTMKAC | 1 cut(s) | 702 |
| Fnu4HI | GCNGC | 4 cut(s) | 1559, 1788, 1999, 2439 |
| FokI | GGATG | 6 cut(s) | 536, 840, 1276, 1349, 1403, 2045 |
| FriOI | GRGCYC | 2 cut(s) | 843, 1666 |
| Fsp4HI | GCNGC | 4 cut(s) | 1559, 1788, 1999, 2439 |
| FspBI | CTAG | 2 cut(s) | 53, 548 |
| GluI | GCNGC | 4 cut(s) | 1559, 1788, 1999, 2439 |
| GsaI | CCCAGC | 1 cut(s) | 1820 |
| GsuI | CTGGAG | 3 cut(s) | 110, 1143, 1722 |
| HaeIII | GGCC | 5 cut(s) | 653, 1085, 1144, 1493, 1664 |
| HapII | CCGG | 2 cut(s) | 148, 160 |
| HgaI | GACGC | 1 cut(s) | 75 |
| Hin1II | CATG | 6 cut(s) | 521, 1416, 1640, 1691, 1933, 2621 |
| HincII | GTYRAC | 2 cut(s) | 1070, 1101 |
| HindII | GTYRAC | 2 cut(s) | 1070, 1101 |
| HindIII | AAGCTT | 1 cut(s) | 932 |
| HpaII | CCGG | 2 cut(s) | 148, 160 |
| HphI | GGTGA | 4 cut(s) | 97, 1440, 2294, 2501 |
| Hpy166II | GTNNAC | 5 cut(s) | 703, 1070, 1101, 2285, 2639 |
| Hpy188I | TCNGA | 8 cut(s) | 19, 34, 229, 321, 1048, 1315, 2116, 2320 |
| Hpy188III | TCNNGA | 9 cut(s) | 160, 182, 425, 749, 1375, 1463, 1619, 1841, 2628 |
| Hpy8I | GTNNAC | 5 cut(s) | 703, 1070, 1101, 2285, 2639 |
| HpyAV | CCTTC | 9 cut(s) | 83, 238, 755, 859, 1401, 1498, 2200, 2272, 2365 |
| HpyCH4III | ACNGT | 8 cut(s) | 135, 690, 707, 896, 1372, 2588, 2636, 2643 |
| HpyCH4IV | ACGT | 4 cut(s) | 343, 806, 2179, 2476 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 1169, 2230, 2389 |
| HpySE526I | ACGT | 4 cut(s) | 343, 806, 2179, 2476 |
| Hsp92II | CATG | 6 cut(s) | 521, 1416, 1640, 1691, 1933, 2621 |
| Kpn2I | TCCGGA | 1 cut(s) | 159 |
| Ksp22I | TGATCA | 2 cut(s) | 1195, 2506 |
| Kzo9I | GATC | 9 cut(s) | 156, 311, 408, 1195, 1284, 1459, 1879, 2068, 2506 |
| LguI | GCTCTTC | 2 cut(s) | 168, 786 |
| LmnI | GCTCC | 4 cut(s) | 541, 574, 1603, 2170 |
| Lsp1109I | GCAGC | 2 cut(s) | 1774, 1985 |
| LweI | GCATC | 5 cut(s) | 23, 514, 1327, 1425, 1792 |
| MaeI | CTAG | 2 cut(s) | 53, 548 |
| MaeII | ACGT | 4 cut(s) | 343, 806, 2179, 2476 |
| MaeIII | GTNAC | 5 cut(s) | 578, 731, 1468, 1564, 1896 |
| MalI | GATC | 9 cut(s) | 158, 313, 410, 1197, 1286, 1461, 1881, 2070, 2508 |
| MbiI | CCGCTC | 1 cut(s) | 2173 |
| MboI | GATC | 9 cut(s) | 156, 311, 408, 1195, 1284, 1459, 1879, 2068, 2506 |
| MflI | RGATCY | 2 cut(s) | 1284, 2068 |
| MhlI | GDGCHC | 2 cut(s) | 843, 1666 |
| MlsI | TGGCCA | 1 cut(s) | 1493 |
| MluNI | TGGCCA | 1 cut(s) | 1493 |
| MmeI | TCCRAC | 8 cut(s) | 180, 207, 596, 1227, 1324, 1407, 1518, 1894 |
| Mox20I | TGGCCA | 1 cut(s) | 1493 |
| Mph1103I | ATGCAT | 1 cut(s) | 1418 |
| MroI | TCCGGA | 1 cut(s) | 159 |
| MroXI | GAANNNNTTC | 2 cut(s) | 177, 1267 |
| MscI | TGGCCA | 1 cut(s) | 1493 |
| MslI | CAYNNNNRTG | 1 cut(s) | 522 |
| Msp20I | TGGCCA | 1 cut(s) | 1493 |
| MspI | CCGG | 2 cut(s) | 148, 160 |
| MspR9I | CCNGG | 3 cut(s) | 128, 148, 2500 |
| Mva1269I | GAATGC | 1 cut(s) | 157 |
| MvaI | CCWGG | 2 cut(s) | 128, 2500 |
| MvnI | CGCG | 1 cut(s) | 1020 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 1169, 2230, 2389 |
| NciI | CCSGG | 1 cut(s) | 148 |
| NdeI | CATATG | 1 cut(s) | 1254 |
| NdeII | GATC | 9 cut(s) | 156, 311, 408, 1195, 1284, 1459, 1879, 2068, 2506 |
| NlaIII | CATG | 6 cut(s) | 521, 1416, 1640, 1691, 1933, 2621 |
| NlaIV | GGNNCC | 1 cut(s) | 1664 |
| NmuCI | GTSAC | 2 cut(s) | 731, 1468 |
| NsiI | ATGCAT | 1 cut(s) | 1418 |
| NspI | RCATGY | 2 cut(s) | 1416, 1691 |
| NspV | TTCGAA | 1 cut(s) | 171 |
| PciSI | GCTCTTC | 2 cut(s) | 168, 786 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 588 |
| PctI | GAATGC | 1 cut(s) | 157 |
| PdmI | GAANNNNTTC | 2 cut(s) | 177, 1267 |
| Pfl23II | CGTACG | 1 cut(s) | 63 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 114, 1500 |
| PfoI | TCCNGGA | 1 cut(s) | 126 |
| PkrI | GCNGC | 4 cut(s) | 1560, 1789, 2000, 2440 |
| PsiI | TTATAA | 1 cut(s) | 1593 |
| Psp124BI | GAGCTC | 1 cut(s) | 843 |
| Psp1406I | AACGTT | 1 cut(s) | 806 |
| Psp6I | CCWGG | 2 cut(s) | 126, 2498 |
| PspFI | CCCAGC | 1 cut(s) | 1816 |
| PspGI | CCWGG | 2 cut(s) | 126, 2498 |
| PspLI | CGTACG | 1 cut(s) | 63 |
| PspN4I | GGNNCC | 1 cut(s) | 1664 |
| PspOMI | GGGCCC | 1 cut(s) | 1662 |
| PspPI | GGNCC | 4 cut(s) | 924, 1142, 1662, 1663 |
| PstI | CTGCAG | 1 cut(s) | 2003 |
| PsuI | RGATCY | 2 cut(s) | 1284, 2068 |
| RsaI | GTAC | 8 cut(s) | 65, 288, 369, 515, 893, 1959, 2250, 2650 |
| RsaNI | GTAC | 8 cut(s) | 64, 287, 368, 514, 892, 1958, 2249, 2649 |
| RseI | CAYNNNNRTG | 1 cut(s) | 522 |
| SacI | GAGCTC | 1 cut(s) | 843 |
| SapI | GCTCTTC | 2 cut(s) | 168, 786 |
| SatI | GCNGC | 4 cut(s) | 1559, 1788, 1999, 2439 |
| Sau3AI | GATC | 9 cut(s) | 156, 311, 408, 1195, 1284, 1459, 1879, 2068, 2506 |
| Sau96I | GGNCC | 4 cut(s) | 924, 1142, 1662, 1663 |
| ScrFI | CCNGG | 3 cut(s) | 128, 148, 2500 |
| SduI | GDGCHC | 2 cut(s) | 843, 1666 |
| SfaNI | GCATC | 5 cut(s) | 23, 514, 1327, 1425, 1792 |
| SfcI | CTRYAG | 1 cut(s) | 1999 |
| SfuI | TTCGAA | 1 cut(s) | 171 |
| SinI | GGWCC | 1 cut(s) | 924 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 522 |
| SmlI | CTYRAG | 4 cut(s) | 749, 1094, 1128, 2108 |
| SmoI | CTYRAG | 4 cut(s) | 749, 1094, 1128, 2108 |
| SsiI | CCGC | 5 cut(s) | 418, 1020, 1558, 2173, 2439 |
| SspI | AATATT | 1 cut(s) | 1213 |
| SspMI | CTAG | 2 cut(s) | 53, 548 |
| SstI | GAGCTC | 1 cut(s) | 843 |
| StyD4I | CCNGG | 3 cut(s) | 126, 146, 2498 |
| StyI | CCWWGG | 2 cut(s) | 207, 2403 |
| TaaI | ACNGT | 8 cut(s) | 135, 690, 707, 896, 1372, 2588, 2636, 2643 |
| TaiI | ACGT | 4 cut(s) | 346, 809, 2182, 2479 |
| TaqI | TCGA | 8 cut(s) | 143, 171, 314, 411, 591, 755, 1374, 2276 |
| TatI | WGTACW | 5 cut(s) | 286, 367, 891, 1957, 2248 |
| TauI | GCSGC | 2 cut(s) | 1561, 2441 |
| TscAI | CASTG | 4 cut(s) | 882, 1501, 2525, 2531 |
| TseFI | GTSAC | 2 cut(s) | 731, 1468 |
| TseI | GCWGC | 2 cut(s) | 1787, 1998 |
| Tsp45I | GTSAC | 2 cut(s) | 731, 1468 |
| TspGWI | ACGGA | 1 cut(s) | 1590 |
| TspRI | CASTG | 4 cut(s) | 882, 1501, 2525, 2531 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 114, 1500 |
| VpaK11BI | GGWCC | 1 cut(s) | 924 |
| XapI | RAATTY | 7 cut(s) | 776, 938, 995, 1033, 1363, 1810, 2537 |
| XceI | RCATGY | 2 cut(s) | 1416, 1691 |
| XmiI | GTMKAC | 1 cut(s) | 702 |
| XmnI | GAANNNNTTC | 2 cut(s) | 177, 1267 |
| XspI | CTAG | 2 cut(s) | 53, 548 |
| Zsp2I | ATGCAT | 1 cut(s) | 1418 |