FvH4_6g10090

YLS9-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0013696
Physical Location & Seq
Forward (+)
5975726 .. 5977340
1615 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g10090.t1

Sequence Viewer

Length: 945 bp
ATGGGTTTACTGAAGTCTCTGCGTCAGAAGATCTTAAGAAAGCTATGTTTTGGCGGAAGCAAGTTCAGCAGTGATTCCAACCACTTCAGGCGACTTGACGATCGTGCCGAAACCAAAACCTGCCGAACTCGGGGAGGTTGTGTACCTGTGTACATCGGTGAACAGAAGATGAGATATGAAGTCCCGCTGCAATATCTCTCTTTTAACAAATTCCAAGATTTGATTCTAGAATCGTTTGGAGGCGATCTTGAATTCGTCAAGAATGAAGGACCTATCGAGCTTCCGAATTGCACCACTGAGAAGTTCTATGAAATATTAAACGAGGCCGAGAAAAACTACAGAAAAATCATGATCGGAAAGTGTTGTTGCATCGTGATCGGCATCATTACCGGATGTGTTCTTATCTTCGGCGTTTCCATTTCAGCCTACATTCTCCATTTTGCCAAGTCCTTGCCTAAGGGGATGGTCCATTATCAGGTCACCAATGCCTCCCTGACCAAGTTCAACCTCACTACCAATAGCACCCTTCTCGATTACGACCTTAAACTCGATCTAACCCTTGAAAACCCTAGCCCCTTCGACTACAACTTCACCCGACTGGATGCCGTGCCGGTTTTCAAGAGCCAAAACCTAAATGACATAACCAATGTTTCGAAACCTTTCGACCTAGTTCACGAGACCAATGTGAAACTCCCGGCATTGTTCTCCGGGAACCATTCGGTGGCTCTAGGCGCTCAGGAGGCTTCTGATTTTGGGAAGGCTGGTGTCTTTGATATTGGTTTGAAGATTAACAGCGAGTTTTTGGCTGAGAAAAAGAAGAAGAAGGTGAGTTTTAAGCATGAGGTGACATGCGACTTGAAGGTCCCTCTGAATTCCAACGGAACAAATGTTGGTGGGAATTTTCAGATTACGAAGTGTAAGGTGGGTAAAGTCGACGTTAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_2

Protein Analysis

315

Amino Acids

35.11

Weight (kDa)

8.87

Isoelectric Point (pI)

25.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Auxin_inducible PF02519 34 - 106 1.1e-07 Auxin responsive protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 860
Acc36I ACCTGC 1 cut(s) 128
AccB7I CCANNNNNTGG 1 cut(s) 721
AccI GTMKAC 1 cut(s) 933
AciI CCGC 2 cut(s) 54, 185
AcsI RAATTY 4 cut(s) 209, 251, 871, 898
AcuI CTGAAG 2 cut(s) 32, 70
AfaI GTAC 2 cut(s) 144, 152
AfiI CCNNNNNNNGG 3 cut(s) 130, 475, 721
AflII CTTAAG 1 cut(s) 34
AgsI TTSAA 6 cut(s) 251, 505, 563, 619, 784, 859
AjuI GAANNNNNNNTTGG 2 cut(s) 207, 239
AluBI AGCT 2 cut(s) 43, 280
AluI AGCT 2 cut(s) 43, 280
Alw26I GTCTC 2 cut(s) 21, 671
Ama87I CYCGRG 1 cut(s) 129
AoxI GGCC 1 cut(s) 324
ApeKI GCWGC 1 cut(s) 187
ApoI RAATTY 4 cut(s) 209, 251, 871, 898
Asp700I GAANNNNTTC 1 cut(s) 659
AspLEI GCGC 1 cut(s) 734
AspS9I GGNCC 3 cut(s) 269, 466, 862
AsuC2I CCSGG 2 cut(s) 695, 709
AsuHPI GGTGA 5 cut(s) 170, 472, 583, 838, 856
AsuII TTCGAA 1 cut(s) 653
AvaI CYCGRG 1 cut(s) 129
AvaII GGWCC 3 cut(s) 269, 466, 862
AxyI CCTNAGG 1 cut(s) 456
BauI CACGAG 1 cut(s) 674
BbvI GCAGC 1 cut(s) 174
BccI CCATC 1 cut(s) 457
BceAI ACGGC 1 cut(s) 590
BcgI CGANNNNNNTGC 4 cut(s) 358, 392, 511, 545
BcnI CCSGG 2 cut(s) 695, 709
BcoDI GTCTC 2 cut(s) 21, 671
BfaI CTAG 4 cut(s) 227, 570, 668, 728
BfmI CTRYAG 1 cut(s) 337
BfoI RGCGCY 1 cut(s) 735
BfrI CTTAAG 1 cut(s) 34
BfuAI ACCTGC 1 cut(s) 128
BglII AGATCT 1 cut(s) 30
BisI GCNGC 1 cut(s) 188
BlsI GCNGC 1 cut(s) 189
Bme1390I CCNGG 2 cut(s) 695, 709
Bme18I GGWCC 3 cut(s) 269, 466, 862
BmeT110I CYCGRG 1 cut(s) 129
BmgT120I GGNCC 3 cut(s) 269, 466, 862
BmiI GGNNCC 2 cut(s) 713, 864
BmrFI CCNGG 2 cut(s) 695, 709
BmsI GCATC 3 cut(s) 378, 390, 592
Bpu10I CCTNAGC 1 cut(s) 735
Bpu14I TTCGAA 1 cut(s) 653
BpuMI CCSGG 2 cut(s) 695, 709
BsaBI GATNNNNATC 1 cut(s) 380
BsaI GGTCTC 1 cut(s) 671
BsaWI WCCGGW 1 cut(s) 389
BsaXI ACNNNNNCTCC 2 cut(s) 126, 156
Bsc4I CCNNNNNNNGG 3 cut(s) 130, 475, 721
Bse118I RCCGGY 1 cut(s) 610
Bse1I ACTGG 1 cut(s) 603
Bse21I CCTNAGG 1 cut(s) 456
Bse8I GATNNNNATC 1 cut(s) 380
BseGI GGATG 3 cut(s) 398, 468, 607
BseJI GATNNNNATC 1 cut(s) 380
BseLI CCNNNNNNNGG 3 cut(s) 130, 475, 721
BseMII CTCAG 3 cut(s) 288, 749, 798
BseNI ACTGG 1 cut(s) 603
BseXI GCAGC 1 cut(s) 174
Bsh1285I CGRYCG 1 cut(s) 103
BshFI GGCC 1 cut(s) 326
BsiEI CGRYCG 1 cut(s) 103
BsiHKCI CYCGRG 1 cut(s) 129
BsiSI CCGG 4 cut(s) 390, 611, 695, 708
BslFI GGGAC 2 cut(s) 167, 848
BslI CCNNNNNNNGG 3 cut(s) 130, 475, 721
BsmAI GTCTC 2 cut(s) 21, 671
BsmFI GGGAC 2 cut(s) 167, 848
BsnI GGCC 1 cut(s) 326
Bso31I GGTCTC 1 cut(s) 671
BsoBI CYCGRG 1 cut(s) 129
Bsp119I TTCGAA 1 cut(s) 653
Bsp1407I TGTACA 1 cut(s) 150
Bsp143I GATC 6 cut(s) 30, 100, 244, 351, 375, 550
BspACI CCGC 2 cut(s) 54, 185
BspANI GGCC 1 cut(s) 326
BspCNI CTCAG 3 cut(s) 289, 748, 799
BspHI TCATGA 1 cut(s) 348
BspLI GGNNCC 2 cut(s) 713, 864
BspMI ACCTGC 1 cut(s) 128
BspT104I TTCGAA 1 cut(s) 653
BspTI CTTAAG 1 cut(s) 34
BspTNI GGTCTC 1 cut(s) 671
BsrFI RCCGGY 1 cut(s) 610
BsrGI TGTACA 1 cut(s) 150
BsrI ACTGG 1 cut(s) 603
BssAI RCCGGY 1 cut(s) 610
BssMI GATC 6 cut(s) 30, 100, 244, 351, 375, 550
BssSI CACGAG 1 cut(s) 674
Bst2BI CACGAG 1 cut(s) 674
BstAFI CTTAAG 1 cut(s) 34
BstAUI TGTACA 1 cut(s) 150
BstBI TTCGAA 1 cut(s) 653
BstDEI CTNAG 4 cut(s) 297, 456, 735, 807
BstEII GGTNACC 1 cut(s) 478
BstF5I GGATG 3 cut(s) 398, 468, 607
BstH2I RGCGCY 1 cut(s) 735
BstHHI GCGC 1 cut(s) 734
BstKTI GATC 6 cut(s) 33, 103, 247, 354, 378, 553
BstMAI GTCTC 2 cut(s) 21, 671
BstMBI GATC 6 cut(s) 30, 100, 244, 351, 375, 550
BstMCI CGRYCG 1 cut(s) 103
BstMWI GCNNNNNNNGC 3 cut(s) 66, 731, 740
BstNSI RCATGY 1 cut(s) 852
BstPI GGTNACC 1 cut(s) 478
BstSCI CCNGG 2 cut(s) 693, 707
BstSFI CTRYAG 1 cut(s) 337
BstV1I GCAGC 1 cut(s) 174
BstX2I RGATCY 1 cut(s) 30
BstYI RGATCY 1 cut(s) 30
Bsu36I CCTNAGG 1 cut(s) 456
BsuRI GGCC 1 cut(s) 326
BtsCI GGATG 3 cut(s) 398, 468, 607
BtsI GCAGTG 1 cut(s) 76
BtsIMutI CAGTG 2 cut(s) 76, 294
BveI ACCTGC 1 cut(s) 128
CciI TCATGA 1 cut(s) 348
CfoI GCGC 1 cut(s) 734
Cfr10I RCCGGY 1 cut(s) 610
Cfr13I GGNCC 3 cut(s) 269, 466, 862
CseI GACGC 1 cut(s) 11
Csp6I GTAC 2 cut(s) 143, 151
CviAII CATG 3 cut(s) 349, 839, 849
CviQI GTAC 2 cut(s) 143, 151
DdeI CTNAG 4 cut(s) 297, 456, 735, 807
DpnI GATC 6 cut(s) 32, 102, 246, 353, 377, 552
DpnII GATC 6 cut(s) 30, 100, 244, 351, 375, 550
DrdI GACNNNNNNGTC 1 cut(s) 860
DseDI GACNNNNNNGTC 1 cut(s) 860
EciI GGCGGA 1 cut(s) 69
Eco31I GGTCTC 1 cut(s) 671
Eco47I GGWCC 3 cut(s) 269, 466, 862
Eco57I CTGAAG 2 cut(s) 32, 70
Eco81I CCTNAGG 1 cut(s) 456
Eco88I CYCGRG 1 cut(s) 129
Eco91I GGTNACC 1 cut(s) 478
EcoO109I RGGNCCY 2 cut(s) 269, 862
EcoO65I GGTNACC 1 cut(s) 478
EcoRI GAATTC 2 cut(s) 251, 871
FaeI CATG 3 cut(s) 352, 842, 852
FaiI YATR 7 cut(s) 46, 177, 309, 350, 641, 840, 850
FaqI GGGAC 2 cut(s) 167, 848
FatI CATG 3 cut(s) 348, 838, 848
FauI CCCGC 1 cut(s) 192
FblI GTMKAC 1 cut(s) 933
Fnu4HI GCNGC 1 cut(s) 188
FokI GGATG 3 cut(s) 405, 475, 614
Fsp4HI GCNGC 1 cut(s) 188
FspBI CTAG 4 cut(s) 227, 570, 668, 728
GlaI GCGC 1 cut(s) 733
GluI GCNGC 1 cut(s) 188
HaeII RGCGCY 1 cut(s) 735
HaeIII GGCC 1 cut(s) 326
HapII CCGG 4 cut(s) 390, 611, 695, 708
HgaI GACGC 1 cut(s) 11
HhaI GCGC 1 cut(s) 734
Hin1II CATG 3 cut(s) 352, 842, 852
Hin6I GCGC 1 cut(s) 732
HinP1I GCGC 1 cut(s) 732
HincII GTYRAC 1 cut(s) 934
HindII GTYRAC 1 cut(s) 934
HinfI GANTC 3 cut(s) 74, 223, 230
HpaII CCGG 4 cut(s) 390, 611, 695, 708
HphI GGTGA 5 cut(s) 170, 472, 583, 838, 856
Hpy166II GTNNAC 6 cut(s) 8, 143, 151, 161, 673, 934
Hpy188I TCNGA 6 cut(s) 27, 285, 356, 748, 870, 906
Hpy188III TCNNGA 9 cut(s) 227, 248, 259, 349, 373, 530, 619, 674, 737
Hpy8I GTNNAC 6 cut(s) 8, 143, 151, 161, 673, 934
Hpy99I CGWCG 1 cut(s) 938
HpyAV CCTTC 6 cut(s) 260, 536, 586, 751, 817, 853
HpyCH4IV ACGT 1 cut(s) 936
HpyCH4V TGCA 3 cut(s) 190, 291, 369
HpyF10VI GCNNNNNNNGC 3 cut(s) 66, 731, 740
HpyF3I CTNAG 4 cut(s) 297, 456, 735, 807
HpySE526I ACGT 1 cut(s) 936
Hsp92II CATG 3 cut(s) 352, 842, 852
HspAI GCGC 1 cut(s) 732
Kzo9I GATC 6 cut(s) 30, 100, 244, 351, 375, 550
Lsp1109I GCAGC 1 cut(s) 174
LweI GCATC 3 cut(s) 378, 390, 592
MaeI CTAG 4 cut(s) 227, 570, 668, 728
MaeII ACGT 1 cut(s) 936
MaeIII GTNAC 2 cut(s) 478, 844
MalI GATC 6 cut(s) 32, 102, 246, 353, 377, 552
MboI GATC 6 cut(s) 30, 100, 244, 351, 375, 550
MboII GAAGA 6 cut(s) 40, 178, 397, 796, 829, 832
MflI RGATCY 1 cut(s) 30
MluCI AATT 6 cut(s) 209, 251, 286, 871, 898, 940
MmeI TCCRAC 2 cut(s) 102, 900
MnlI CCTC 8 cut(s) 128, 233, 316, 499, 518, 733, 835, 876
MroXI GAANNNNTTC 1 cut(s) 659
MseI TTAA 7 cut(s) 35, 204, 317, 543, 789, 834, 939
MspA1I CMGCKG 1 cut(s) 187
MspCI CTTAAG 1 cut(s) 34
MspI CCGG 4 cut(s) 390, 611, 695, 708
MspR9I CCNGG 2 cut(s) 695, 709
MwoI GCNNNNNNNGC 3 cut(s) 66, 731, 740
NciI CCSGG 2 cut(s) 695, 709
NdeII GATC 6 cut(s) 30, 100, 244, 351, 375, 550
NlaIII CATG 3 cut(s) 352, 842, 852
NlaIV GGNNCC 2 cut(s) 713, 864
NmeAIII GCCGAG 1 cut(s) 352
NmuCI GTSAC 2 cut(s) 478, 844
NspI RCATGY 1 cut(s) 852
NspV TTCGAA 1 cut(s) 653
PagI TCATGA 1 cut(s) 348
PcsI WCGNNNNNNNCGW 1 cut(s) 105
PdmI GAANNNNTTC 1 cut(s) 659
PfeI GAWTC 3 cut(s) 74, 223, 230
PflMI CCANNNNNTGG 1 cut(s) 721
PfoI TCCNGGA 1 cut(s) 707
PkrI GCNGC 1 cut(s) 189
Ple19I CGATCG 1 cut(s) 103
PpuMI RGGWCCY 2 cut(s) 269, 862
Psp5II RGGWCCY 2 cut(s) 269, 862
PspEI GGTNACC 1 cut(s) 478
PspN4I GGNNCC 2 cut(s) 713, 864
PspPI GGNCC 3 cut(s) 269, 466, 862
PspPPI RGGWCCY 2 cut(s) 269, 862
PsuI RGATCY 1 cut(s) 30
PvuI CGATCG 1 cut(s) 103
RsaI GTAC 2 cut(s) 144, 152
RsaNI GTAC 2 cut(s) 143, 151
SalI GTCGAC 1 cut(s) 932
SaqAI TTAA 7 cut(s) 35, 204, 317, 543, 789, 834, 939
SatI GCNGC 1 cut(s) 188
Sau3AI GATC 6 cut(s) 30, 100, 244, 351, 375, 550
Sau96I GGNCC 3 cut(s) 269, 466, 862
ScrFI CCNGG 2 cut(s) 695, 709
SfaNI GCATC 3 cut(s) 378, 390, 592
SfcI CTRYAG 1 cut(s) 337
SfuI TTCGAA 1 cut(s) 653
SinI GGWCC 3 cut(s) 269, 466, 862
SmlI CTYRAG 1 cut(s) 34
SmoI CTYRAG 1 cut(s) 34
Sse9I AATT 6 cut(s) 209, 251, 286, 871, 898, 940
SsiI CCGC 2 cut(s) 54, 185
SspI AATATT 1 cut(s) 315
SspMI CTAG 4 cut(s) 227, 570, 668, 728
StyD4I CCNGG 2 cut(s) 693, 707
TaiI ACGT 1 cut(s) 939
TaqI TCGA 7 cut(s) 276, 531, 549, 579, 653, 663, 933
TasI AATT 6 cut(s) 209, 251, 286, 871, 898, 940
TatI WGTACW 1 cut(s) 150
TfiI GAWTC 3 cut(s) 74, 223, 230
Tru1I TTAA 7 cut(s) 35, 204, 317, 543, 789, 834, 939
Tru9I TTAA 7 cut(s) 35, 204, 317, 543, 789, 834, 939
TscAI CASTG 2 cut(s) 76, 301
TseFI GTSAC 2 cut(s) 478, 844
TseI GCWGC 1 cut(s) 187
Tsp45I GTSAC 2 cut(s) 478, 844
TspDTI ATGAA 3 cut(s) 192, 279, 324
TspGWI ACGGA 1 cut(s) 894
TspRI CASTG 2 cut(s) 76, 301
Van91I CCANNNNNTGG 1 cut(s) 721
Vha464I CTTAAG 1 cut(s) 34
VpaK11BI GGWCC 3 cut(s) 269, 466, 862
XapI RAATTY 4 cut(s) 209, 251, 871, 898
XbaI TCTAGA 1 cut(s) 226
XceI RCATGY 1 cut(s) 852
XmiI GTMKAC 1 cut(s) 933
XmnI GAANNNNTTC 1 cut(s) 659
XspI CTAG 4 cut(s) 227, 570, 668, 728
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.