Prupe.6G291900_v2.0.a1

YLS9-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0013696
Physical Location & Seq
Forward (+)
26909462 .. 26910058
597 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G291900.1

Sequence Viewer

Length: 597 bp
ATGGGTATGTGCACTTTTTGCTGTTGCTCCATCATCCTTGTGGCCCTAGTTTTTGCCCTAGGCATCACTGGTTTTGTGTCCTGGATCATAGCTGGCTTTGTTCGTGTTTCTCATTTGAAACGCATCAACTACCAAGTGACCGATGCCTCACTTCTCCAATTCAACGTGACAAACAATAACACCACCCTCGAATACAATCTAGCCCTCAACATAACCCTTCAAAACCCTAACGACAAGATTGGTCTCCACTTTGACCGAATCGAAGCCAGTGCCATTTTCATGAAGCAAACCCTAAATAATGTTAGCTTGAGCTCCTCCTCCTACCTAGGGCACAAGAAGACGGTTTTATTACCAGCATTGTTCAAGGGGCAGCGACAAGTGGCTCTCAGGGCTGATGAGGTCTCCAACTTTAAGAATGCTGGGGATTTTGATATCATGTTGAACCTGGATGTTAAGTACTGGGTCAAGTTTCTTGCATCGAAGGTTAAAATTAAGCAACCCCATATAGCATGCTCTTTGAAGGTCCCTCTAAACAACGGGAAACCTGCCGAAGGTTTTAAGGTTACGCAATGCAAAGTTCAATCATCGGACAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_2

Protein Analysis

199

Amino Acids

22.0

Weight (kDa)

9.45

Isoelectric Point (pI)

26.52

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 553
AclWI GGATC 1 cut(s) 92
AfaI GTAC 1 cut(s) 458
AfiI CCNNNNNNNGG 2 cut(s) 327, 551
AgsI TTSAA 7 cut(s) 118, 163, 221, 364, 442, 520, 581
AjnI CCWGG 2 cut(s) 80, 444
AluBI AGCT 3 cut(s) 92, 306, 312
AluI AGCT 3 cut(s) 92, 306, 312
Alw21I GWGCWC 2 cut(s) 14, 314
Alw26I GTCTC 2 cut(s) 248, 406
Alw44I GTGCAC 1 cut(s) 10
AlwI GGATC 1 cut(s) 92
AoxI GGCC 1 cut(s) 42
ApaLI GTGCAC 1 cut(s) 10
ApeKI GCWGC 1 cut(s) 370
AspA2I CCTAGG 2 cut(s) 58, 325
AspS9I GGNCC 2 cut(s) 43, 523
AvaII GGWCC 1 cut(s) 523
AvrII CCTAGG 2 cut(s) 58, 325
BaeGI GKGCMC 2 cut(s) 14, 333
BanII GRGCYC 1 cut(s) 314
BbsI GAAGAC 1 cut(s) 344
Bbv12I GWGCWC 2 cut(s) 14, 314
BbvI GCAGC 1 cut(s) 382
BccI CCATC 1 cut(s) 38
BcgI CGANNNNNNTGC 2 cut(s) 251, 285
BciT130I CCWGG 2 cut(s) 82, 446
BcoDI GTCTC 2 cut(s) 248, 406
BfaI CTAG 4 cut(s) 47, 59, 200, 326
BfuAI ACCTGC 1 cut(s) 553
BisI GCNGC 1 cut(s) 371
BlnI CCTAGG 2 cut(s) 58, 325
BlsI GCNGC 1 cut(s) 372
BmcAI AGTACT 1 cut(s) 458
Bme1390I CCNGG 2 cut(s) 82, 446
Bme18I GGWCC 1 cut(s) 523
BmgT120I GGNCC 2 cut(s) 43, 523
BmiI GGNNCC 1 cut(s) 525
BmrFI CCNGG 2 cut(s) 82, 446
BmrI ACTGGG 1 cut(s) 469
BmsI GCATC 4 cut(s) 72, 132, 133, 485
BmuI ACTGGG 1 cut(s) 469
BpiI GAAGAC 1 cut(s) 344
BpuEI CTTGAG 1 cut(s) 328
BsaI GGTCTC 2 cut(s) 248, 406
BsaJI CCNNGG 2 cut(s) 58, 325
Bsc4I CCNNNNNNNGG 2 cut(s) 327, 551
Bse1I ACTGG 3 cut(s) 73, 267, 464
Bse3DI GCAATG 1 cut(s) 575
BseBI CCWGG 2 cut(s) 82, 446
BseDI CCNNGG 2 cut(s) 58, 325
BseGI GGATG 2 cut(s) 33, 454
BseLI CCNNNNNNNGG 2 cut(s) 327, 551
BseMI GCAATG 1 cut(s) 575
BseMII CTCAG 1 cut(s) 400
BseNI ACTGG 3 cut(s) 73, 267, 464
BseRI GAGGAG 2 cut(s) 304, 307
BseSI GKGCMC 2 cut(s) 14, 333
BseXI GCAGC 1 cut(s) 382
BseYI CCCAGC 1 cut(s) 419
BshFI GGCC 1 cut(s) 44
BsiHKAI GWGCWC 2 cut(s) 14, 314
BslFI GGGAC 1 cut(s) 509
BslI CCNNNNNNNGG 2 cut(s) 327, 551
BsmAI GTCTC 2 cut(s) 248, 406
BsmFI GGGAC 1 cut(s) 509
BsmI GAATGC 1 cut(s) 421
BsnI GGCC 1 cut(s) 44
Bso31I GGTCTC 2 cut(s) 248, 406
Bsp1286I GDGCHC 3 cut(s) 14, 314, 333
Bsp143I GATC 1 cut(s) 84
BspANI GGCC 1 cut(s) 44
BspCNI CTCAG 1 cut(s) 399
BspHI TCATGA 1 cut(s) 279
BspLI GGNNCC 1 cut(s) 525
BspMI ACCTGC 1 cut(s) 553
BspPI GGATC 1 cut(s) 92
BspTNI GGTCTC 2 cut(s) 248, 406
BsrDI GCAATG 1 cut(s) 575
BsrI ACTGG 3 cut(s) 73, 267, 464
BssECI CCNNGG 2 cut(s) 58, 325
BssMI GATC 1 cut(s) 84
BssT1I CCWWGG 2 cut(s) 58, 325
Bst2UI CCWGG 2 cut(s) 82, 446
Bst4CI ACNGT 1 cut(s) 343
BstAPI GCANNNNNTGC 1 cut(s) 18
BstC8I GCNNGC 2 cut(s) 94, 511
BstDEI CTNAG 1 cut(s) 386
BstENI CCTNNNNNAGG 1 cut(s) 549
BstF5I GGATG 2 cut(s) 33, 454
BstKTI GATC 1 cut(s) 87
BstMAI GTCTC 2 cut(s) 248, 406
BstMBI GATC 1 cut(s) 84
BstMWI GCNNNNNNNGC 2 cut(s) 18, 389
BstNI CCWGG 2 cut(s) 82, 446
BstNSI RCATGY 1 cut(s) 513
BstSCI CCNGG 2 cut(s) 80, 444
BstSLI GKGCMC 2 cut(s) 14, 333
BstV1I GCAGC 1 cut(s) 382
BstV2I GAAGAC 1 cut(s) 344
BsuRI GGCC 1 cut(s) 44
BtsCI GGATG 2 cut(s) 33, 454
BtsIMutI CAGTG 2 cut(s) 66, 274
BveI ACCTGC 1 cut(s) 553
Cac8I GCNNGC 2 cut(s) 94, 511
CciI TCATGA 1 cut(s) 279
Cfr13I GGNCC 2 cut(s) 43, 523
Csp6I GTAC 1 cut(s) 457
CviAII CATG 3 cut(s) 280, 436, 510
CviJI RGCY 9 cut(s) 44, 92, 96, 203, 266, 306, 312, 383, 392
CviKI_1 RGCY 9 cut(s) 44, 92, 96, 203, 266, 306, 312, 383, 392
CviQI GTAC 1 cut(s) 457
DdeI CTNAG 1 cut(s) 386
DpnI GATC 1 cut(s) 86
DpnII GATC 1 cut(s) 84
Ecl136II GAGCTC 1 cut(s) 312
Eco130I CCWWGG 2 cut(s) 58, 325
Eco24I GRGCYC 1 cut(s) 314
Eco31I GGTCTC 2 cut(s) 248, 406
Eco32I GATATC 1 cut(s) 433
Eco47I GGWCC 1 cut(s) 523
Eco53kI GAGCTC 1 cut(s) 312
EcoICRI GAGCTC 1 cut(s) 312
EcoNI CCTNNNNNAGG 1 cut(s) 549
EcoO109I RGGNCCY 1 cut(s) 523
EcoRII CCWGG 2 cut(s) 80, 444
EcoRV GATATC 1 cut(s) 433
EcoT14I CCWWGG 2 cut(s) 58, 325
EcoT38I GRGCYC 1 cut(s) 314
ErhI CCWWGG 2 cut(s) 58, 325
FaeI CATG 3 cut(s) 283, 439, 513
FaiI YATR 8 cut(s) 8, 89, 212, 281, 437, 504, 506, 511
FaqI GGGAC 1 cut(s) 509
FatI CATG 3 cut(s) 279, 435, 509
Fnu4HI GCNGC 1 cut(s) 371
FokI GGATG 2 cut(s) 20, 461
FriOI GRGCYC 1 cut(s) 314
Fsp4HI GCNGC 1 cut(s) 371
FspBI CTAG 4 cut(s) 47, 59, 200, 326
GluI GCNGC 1 cut(s) 371
GsaI CCCAGC 1 cut(s) 423
HaeIII GGCC 1 cut(s) 44
Hin1II CATG 3 cut(s) 283, 439, 513
HinfI GANTC 1 cut(s) 258
Hpy166II GTNNAC 1 cut(s) 12
Hpy188I TCNGA 1 cut(s) 589
Hpy188III TCNNGA 1 cut(s) 280
Hpy8I GTNNAC 1 cut(s) 12
HpyAV CCTTC 4 cut(s) 227, 475, 514, 545
HpyCH4III ACNGT 1 cut(s) 343
HpyCH4IV ACGT 1 cut(s) 165
HpyCH4V TGCA 3 cut(s) 12, 476, 573
HpyF10VI GCNNNNNNNGC 2 cut(s) 18, 389
HpyF3I CTNAG 1 cut(s) 386
HpySE526I ACGT 1 cut(s) 165
Hsp92II CATG 3 cut(s) 283, 439, 513
Kzo9I GATC 1 cut(s) 84
LmnI GCTCC 2 cut(s) 32, 317
Lsp1109I GCAGC 1 cut(s) 382
LweI GCATC 4 cut(s) 72, 132, 133, 485
MaeI CTAG 4 cut(s) 47, 59, 200, 326
MaeII ACGT 1 cut(s) 165
MaeIII GTNAC 3 cut(s) 136, 166, 562
MalI GATC 1 cut(s) 86
MboI GATC 1 cut(s) 84
MboII GAAGA 1 cut(s) 349
MhlI GDGCHC 3 cut(s) 14, 314, 333
MluCI AATT 2 cut(s) 158, 489
MmeI TCCRAC 1 cut(s) 429
MnlI CCTC 7 cut(s) 157, 197, 215, 325, 328, 391, 537
MseI TTAA 5 cut(s) 411, 453, 486, 492, 558
MslI CAYNNNNRTG 2 cut(s) 38, 278
MspR9I CCNGG 2 cut(s) 82, 446
Mva1269I GAATGC 1 cut(s) 421
MvaI CCWGG 2 cut(s) 82, 446
MwoI GCNNNNNNNGC 2 cut(s) 18, 389
NdeII GATC 1 cut(s) 84
NlaIII CATG 3 cut(s) 283, 439, 513
NlaIV GGNNCC 1 cut(s) 525
NmuCI GTSAC 2 cut(s) 136, 166
NspI RCATGY 1 cut(s) 513
PaeI GCATGC 1 cut(s) 513
PagI TCATGA 1 cut(s) 279
PctI GAATGC 1 cut(s) 421
PfeI GAWTC 1 cut(s) 258
PfoI TCCNGGA 1 cut(s) 80
PkrI GCNGC 1 cut(s) 372
PpuMI RGGWCCY 1 cut(s) 523
Psp124BI GAGCTC 1 cut(s) 314
Psp5II RGGWCCY 1 cut(s) 523
Psp6I CCWGG 2 cut(s) 80, 444
PspFI CCCAGC 1 cut(s) 419
PspGI CCWGG 2 cut(s) 80, 444
PspN4I GGNNCC 1 cut(s) 525
PspPI GGNCC 2 cut(s) 43, 523
PspPPI RGGWCCY 1 cut(s) 523
RsaI GTAC 1 cut(s) 458
RsaNI GTAC 1 cut(s) 457
RseI CAYNNNNRTG 2 cut(s) 38, 278
SacI GAGCTC 1 cut(s) 314
SaqAI TTAA 5 cut(s) 411, 453, 486, 492, 558
SatI GCNGC 1 cut(s) 371
Sau3AI GATC 1 cut(s) 84
Sau96I GGNCC 2 cut(s) 43, 523
ScaI AGTACT 1 cut(s) 458
ScrFI CCNGG 2 cut(s) 82, 446
SduI GDGCHC 3 cut(s) 14, 314, 333
SfaNI GCATC 4 cut(s) 72, 132, 133, 485
SinI GGWCC 1 cut(s) 523
SmiMI CAYNNNNRTG 2 cut(s) 38, 278
SmlI CTYRAG 1 cut(s) 307
SmoI CTYRAG 1 cut(s) 307
SphI GCATGC 1 cut(s) 513
Sse9I AATT 2 cut(s) 158, 489
SspMI CTAG 4 cut(s) 47, 59, 200, 326
SstI GAGCTC 1 cut(s) 314
StyD4I CCNGG 2 cut(s) 80, 444
StyI CCWWGG 2 cut(s) 58, 325
TaaI ACNGT 1 cut(s) 343
TaiI ACGT 1 cut(s) 168
TaqI TCGA 3 cut(s) 189, 261, 479
TaqII GACCGA 2 cut(s) 155, 270
TasI AATT 2 cut(s) 158, 489
TatI WGTACW 1 cut(s) 456
TfiI GAWTC 1 cut(s) 258
Tru1I TTAA 5 cut(s) 411, 453, 486, 492, 558
Tru9I TTAA 5 cut(s) 411, 453, 486, 492, 558
TscAI CASTG 2 cut(s) 73, 274
TseFI GTSAC 2 cut(s) 136, 166
TseI GCWGC 1 cut(s) 370
Tsp45I GTSAC 2 cut(s) 136, 166
TspDTI ATGAA 2 cut(s) 268, 296
TspRI CASTG 2 cut(s) 73, 274
VneI GTGCAC 1 cut(s) 10
VpaK11BI GGWCC 1 cut(s) 523
XagI CCTNNNNNAGG 1 cut(s) 549
XceI RCATGY 1 cut(s) 513
XcmI CCANNNNNNNNNTGG 1 cut(s) 37
XmaJI CCTAGG 2 cut(s) 58, 325
XspI CTAG 4 cut(s) 47, 59, 200, 326
ZrmI AGTACT 1 cut(s) 458
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.