MD12G1035100.v1.1

YLS9-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0013696
Physical Location & Seq
Forward (+)
3838291 .. 3838863
573 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1035100.v1.1.491

Sequence Viewer

Length: 573 bp
ATGCTAAGAAAATGTTGCTGCTGTTTCTGCATCATTGTTGTAGTACTCGGTGCCCTCGCGTTCACCGCTTGGTTCGTGATTCGTCAATTCAGCCCTTCTAAACCGATGATTTACCAGGTCACCGATGCCTTGCTGACGCAATTCAACCTGACGGCAACCAACAACACCATCTTTTACAATCTGTCCCTCAAGATGACTGTGGAAAACCGTAACAAATGGAATGACTTCCACTATGAACATTTTGAAGCCGTTCCTAGTTATAAGAACCAGGATTTGAGTACCAGTATTCTAGGACCATTCGGCGTAGCGCACAGGGATAAGTACGAGTTGAATCCATTGTTCAAAGGACACCGAACGGTGACGGCTCTTAGAAATGGGGAGGCTTCAAATTTTAGGAATAGTTTGGTTTTTGATATCGCGTTGAAGATCTATTTTAAGTATTGGACCAAGATTGCTATGTACAAGGATGAGAAGGAGCTCCAGATGGCTTGCTATTTGAAGGTTCCATTGAGTTCTAGCGGAAAACCAGCTGAAATTTTTCAGAGCACCAAGTGTGACAAGGTCGACCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_2

Protein Analysis

191

Amino Acids

21.97

Weight (kDa)

9.03

Isoelectric Point (pI)

26.25

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 261
AccB1I GGYRCC 1 cut(s) 50
AccI GTMKAC 1 cut(s) 564
AccII CGCG 2 cut(s) 59, 419
AciI CCGC 2 cut(s) 66, 519
AcsI RAATTY 2 cut(s) 388, 534
AdeI CACNNNGTG 1 cut(s) 552
AfaI GTAC 4 cut(s) 45, 280, 323, 461
AgsI TTSAA 7 cut(s) 145, 245, 331, 343, 387, 424, 499
AjnI CCWGG 2 cut(s) 114, 267
AluBI AGCT 2 cut(s) 478, 530
AluI AGCT 2 cut(s) 478, 530
Alw21I GWGCWC 2 cut(s) 480, 548
ApeKI GCWGC 1 cut(s) 18
ApoI RAATTY 2 cut(s) 388, 534
Asp700I GAANNNNTTC 3 cut(s) 224, 249, 537
AspLEI GCGC 1 cut(s) 310
AspS9I GGNCC 2 cut(s) 293, 444
AsuHPI GGTGA 3 cut(s) 55, 112, 370
AvaII GGWCC 2 cut(s) 293, 444
BaeGI GKGCMC 1 cut(s) 55
BanI GGYRCC 1 cut(s) 50
BanII GRGCYC 1 cut(s) 480
Bbv12I GWGCWC 2 cut(s) 480, 548
BbvI GCAGC 1 cut(s) 5
BccI CCATC 2 cut(s) 176, 478
BceAI ACGGC 3 cut(s) 168, 233, 378
BciT130I CCWGG 2 cut(s) 116, 269
BfaI CTAG 3 cut(s) 255, 290, 516
BglII AGATCT 1 cut(s) 426
BisI GCNGC 1 cut(s) 19
BlsI GCNGC 1 cut(s) 20
BmcAI AGTACT 1 cut(s) 45
Bme1390I CCNGG 2 cut(s) 116, 269
Bme18I GGWCC 2 cut(s) 293, 444
BmgT120I GGNCC 2 cut(s) 293, 444
BmiI GGNNCC 2 cut(s) 52, 504
BmrFI CCNGG 2 cut(s) 116, 269
BmsI GCATC 2 cut(s) 39, 115
BpmI CTGGAG 1 cut(s) 464
BpuEI CTTGAG 1 cut(s) 173
Bse1I ACTGG 1 cut(s) 282
BseBI CCWGG 2 cut(s) 116, 269
BseGI GGATG 1 cut(s) 472
BseNI ACTGG 1 cut(s) 282
BseSI GKGCMC 1 cut(s) 55
BseXI GCAGC 1 cut(s) 5
Bsh1236I CGCG 2 cut(s) 59, 419
BshNI GGYRCC 1 cut(s) 50
BsiHKAI GWGCWC 2 cut(s) 480, 548
BslFI GGGAC 1 cut(s) 169
BsmFI GGGAC 1 cut(s) 169
Bsp1286I GDGCHC 3 cut(s) 55, 480, 548
Bsp1407I TGTACA 1 cut(s) 459
Bsp143I GATC 1 cut(s) 426
BspACI CCGC 2 cut(s) 66, 519
BspFNI CGCG 2 cut(s) 59, 419
BspLI GGNNCC 2 cut(s) 52, 504
BspT107I GGYRCC 1 cut(s) 50
BsrGI TGTACA 1 cut(s) 459
BsrI ACTGG 1 cut(s) 282
BssMI GATC 1 cut(s) 426
Bst2UI CCWGG 2 cut(s) 116, 269
Bst4CI ACNGT 3 cut(s) 199, 209, 358
BstAUI TGTACA 1 cut(s) 459
BstC8I GCNNGC 1 cut(s) 490
BstDEI CTNAG 2 cut(s) 5, 368
BstEII GGTNACC 1 cut(s) 118
BstF5I GGATG 1 cut(s) 472
BstFNI CGCG 2 cut(s) 59, 419
BstHHI GCGC 1 cut(s) 310
BstKTI GATC 1 cut(s) 429
BstMBI GATC 1 cut(s) 426
BstMWI GCNNNNNNNGC 2 cut(s) 27, 65
BstNI CCWGG 2 cut(s) 116, 269
BstPI GGTNACC 1 cut(s) 118
BstSCI CCNGG 2 cut(s) 114, 267
BstSLI GKGCMC 1 cut(s) 55
BstUI CGCG 2 cut(s) 59, 419
BstV1I GCAGC 1 cut(s) 5
BstX2I RGATCY 1 cut(s) 426
BstYI RGATCY 1 cut(s) 426
BtsCI GGATG 1 cut(s) 472
Cac8I GCNNGC 1 cut(s) 490
CfoI GCGC 1 cut(s) 310
Cfr13I GGNCC 2 cut(s) 293, 444
CseI GACGC 1 cut(s) 145
CsiI ACCWGGT 1 cut(s) 114
Csp6I GTAC 4 cut(s) 44, 279, 322, 460
CviJI RGCY 7 cut(s) 93, 248, 365, 383, 478, 488, 530
CviKI_1 RGCY 7 cut(s) 93, 248, 365, 383, 478, 488, 530
CviQI GTAC 4 cut(s) 44, 279, 322, 460
DdeI CTNAG 2 cut(s) 5, 368
DpnI GATC 1 cut(s) 428
DpnII GATC 1 cut(s) 426
DraIII CACNNNGTG 1 cut(s) 552
Ecl136II GAGCTC 1 cut(s) 478
Eco24I GRGCYC 1 cut(s) 480
Eco32I GATATC 1 cut(s) 415
Eco47I GGWCC 2 cut(s) 293, 444
Eco53kI GAGCTC 1 cut(s) 478
Eco91I GGTNACC 1 cut(s) 118
EcoICRI GAGCTC 1 cut(s) 478
EcoO65I GGTNACC 1 cut(s) 118
EcoRII CCWGG 2 cut(s) 114, 267
EcoRV GATATC 1 cut(s) 415
EcoT38I GRGCYC 1 cut(s) 480
FaiI YATR 3 cut(s) 234, 261, 458
FaqI GGGAC 1 cut(s) 169
FblI GTMKAC 1 cut(s) 564
Fnu4HI GCNGC 1 cut(s) 19
FokI GGATG 1 cut(s) 479
FriOI GRGCYC 1 cut(s) 480
Fsp4HI GCNGC 1 cut(s) 19
FspBI CTAG 3 cut(s) 255, 290, 516
GlaI GCGC 1 cut(s) 309
GluI GCNGC 1 cut(s) 19
GsuI CTGGAG 1 cut(s) 464
HgaI GACGC 1 cut(s) 145
HhaI GCGC 1 cut(s) 310
Hin6I GCGC 1 cut(s) 308
HinP1I GCGC 1 cut(s) 308
HincII GTYRAC 1 cut(s) 565
HindII GTYRAC 1 cut(s) 565
HinfI GANTC 2 cut(s) 79, 331
HphI GGTGA 3 cut(s) 55, 112, 370
Hpy166II GTNNAC 2 cut(s) 63, 565
Hpy188I TCNGA 1 cut(s) 543
Hpy188III TCNNGA 3 cut(s) 76, 190, 481
Hpy8I GTNNAC 2 cut(s) 63, 565
HpyAV CCTTC 3 cut(s) 105, 466, 493
HpyCH4III ACNGT 3 cut(s) 199, 209, 358
HpyCH4V TGCA 1 cut(s) 30
HpyF10VI GCNNNNNNNGC 2 cut(s) 27, 65
HpyF3I CTNAG 2 cut(s) 5, 368
HspAI GCGC 1 cut(s) 308
Kzo9I GATC 1 cut(s) 426
LmnI GCTCC 2 cut(s) 475, 483
LpnPI CCDG 9 cut(s) 101, 128, 161, 254, 281, 295, 298, 494, 540
Lsp1109I GCAGC 1 cut(s) 5
LweI GCATC 2 cut(s) 39, 115
MabI ACCWGGT 1 cut(s) 114
MaeI CTAG 3 cut(s) 255, 290, 516
MaeIII GTNAC 4 cut(s) 118, 209, 358, 554
MalI GATC 1 cut(s) 428
MboI GATC 1 cut(s) 426
MboII GAAGA 1 cut(s) 436
MflI RGATCY 1 cut(s) 426
MhlI GDGCHC 3 cut(s) 55, 480, 548
MluCI AATT 4 cut(s) 86, 140, 388, 534
MnlI CCTC 3 cut(s) 65, 197, 373
MroXI GAANNNNTTC 3 cut(s) 224, 249, 537
MseI TTAA 2 cut(s) 435, 571
MspA1I CMGCKG 1 cut(s) 530
MspR9I CCNGG 2 cut(s) 116, 269
MvaI CCWGG 2 cut(s) 116, 269
MvnI CGCG 2 cut(s) 59, 419
MwoI GCNNNNNNNGC 2 cut(s) 27, 65
NdeII GATC 1 cut(s) 426
NlaIV GGNNCC 2 cut(s) 52, 504
NmuCI GTSAC 3 cut(s) 118, 358, 554
PdmI GAANNNNTTC 3 cut(s) 224, 249, 537
PfeI GAWTC 2 cut(s) 79, 331
PflFI GACNNNGTC 1 cut(s) 560
PkrI GCNGC 1 cut(s) 20
PsiI TTATAA 1 cut(s) 261
Psp124BI GAGCTC 1 cut(s) 480
Psp6I CCWGG 2 cut(s) 114, 267
PspEI GGTNACC 1 cut(s) 118
PspGI CCWGG 2 cut(s) 114, 267
PspN4I GGNNCC 2 cut(s) 52, 504
PspPI GGNCC 2 cut(s) 293, 444
PsuI RGATCY 1 cut(s) 426
PsyI GACNNNGTC 1 cut(s) 560
PvuII CAGCTG 1 cut(s) 530
RsaI GTAC 4 cut(s) 45, 280, 323, 461
RsaNI GTAC 4 cut(s) 44, 279, 322, 460
SacI GAGCTC 1 cut(s) 480
SalI GTCGAC 1 cut(s) 563
SaqAI TTAA 2 cut(s) 435, 571
SatI GCNGC 1 cut(s) 19
Sau3AI GATC 1 cut(s) 426
Sau96I GGNCC 2 cut(s) 293, 444
ScaI AGTACT 1 cut(s) 45
ScrFI CCNGG 2 cut(s) 116, 269
SduI GDGCHC 3 cut(s) 55, 480, 548
SetI ASST 6 cut(s) 120, 150, 480, 504, 532, 564
SexAI ACCWGGT 1 cut(s) 114
SfaNI GCATC 2 cut(s) 39, 115
SinI GGWCC 2 cut(s) 293, 444
SmlI CTYRAG 1 cut(s) 188
SmoI CTYRAG 1 cut(s) 188
Sse9I AATT 4 cut(s) 86, 140, 388, 534
SsiI CCGC 2 cut(s) 66, 519
SspMI CTAG 3 cut(s) 255, 290, 516
SstI GAGCTC 1 cut(s) 480
StyD4I CCNGG 2 cut(s) 114, 267
TaaI ACNGT 3 cut(s) 199, 209, 358
TaqI TCGA 1 cut(s) 564
TasI AATT 4 cut(s) 86, 140, 388, 534
TatI WGTACW 2 cut(s) 43, 459
TfiI GAWTC 2 cut(s) 79, 331
Tru1I TTAA 2 cut(s) 435, 571
Tru9I TTAA 2 cut(s) 435, 571
TseFI GTSAC 3 cut(s) 118, 358, 554
TseI GCWGC 1 cut(s) 18
Tsp45I GTSAC 3 cut(s) 118, 358, 554
TspDTI ATGAA 1 cut(s) 249
Tth111I GACNNNGTC 1 cut(s) 560
VpaK11BI GGWCC 2 cut(s) 293, 444
XapI RAATTY 2 cut(s) 388, 534
XmiI GTMKAC 1 cut(s) 564
XmnI GAANNNNTTC 3 cut(s) 224, 249, 537
XspI CTAG 3 cut(s) 255, 290, 516
ZrmI AGTACT 1 cut(s) 45
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.