MD14G1021900.v1.1

YLS9-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0013696
Physical Location & Seq
Forward (+)
2025868 .. 2026437
570 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1021900.v1.1.491

Sequence Viewer

Length: 570 bp
ATGGCTTGCAATTGGAAAAAATGTTGCTGCTTTTGTTGCATCCTCATGATAGTTTTCATCATTGCCCTCAGCGTCGCTGTTATCGTTGTTCGCGAATACAGCCCTAAACGTGGGATCAAGTACGAGGTGACTGATGCGTCTCTGACCCAATTCAACCTGACGAGCGATAAAATCCTCAAATTCAACTTAGCGGTCACGATAAATGTCGAAAACCCTAACAAAAGGTCGGACTTCCACTACGAGAAATTCGAAGCCGTTGCTTCTTACAAGCACGACAGTTTAATGAGCGTTAGCATCGACCCCTTCGAGGTAGAGCACAAGGATAAGCATCCGGTGAGTGCATTGTTCAAGGGGGAGCAAAAGATGTCTCTTCCGAATGATGAGGTTTCAAAGATGAAGACTAGTACGGTTTATGATATCGTTTTGAAGTTGAAGGTCAAGCATTGGGCAAAGTATGCTACAATCAAGATTCAAGAGAATATGAAGATGGCATGCAACTTGAAGGTCCCTTTGAATTCTAACGGAAAATCTGCTGTAAAATTTGAGGTTGCCAAGTGTGAGAAGGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_2

Protein Analysis

190

Amino Acids

21.51

Weight (kDa)

9.1

Isoelectric Point (pI)

33.56

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 136
AccII CGCG 1 cut(s) 93
AciI CCGC 1 cut(s) 191
AclWI GGATC 1 cut(s) 122
AcsI RAATTY 4 cut(s) 179, 245, 514, 539
AfaI GTAC 2 cut(s) 122, 406
AfiI CCNNNNNNNGG 2 cut(s) 110, 307
AgsI TTSAA 9 cut(s) 154, 184, 349, 390, 427, 433, 473, 502, 514
AhlI ACTAGT 1 cut(s) 401
Alw21I GWGCWC 1 cut(s) 318
Alw26I GTCTC 2 cut(s) 144, 372
AlwI GGATC 1 cut(s) 122
ApeKI GCWGC 1 cut(s) 27
ApoI RAATTY 4 cut(s) 179, 245, 514, 539
AspS9I GGNCC 1 cut(s) 505
AsuHPI GGTGA 2 cut(s) 139, 346
AsuII TTCGAA 1 cut(s) 249
AvaII GGWCC 1 cut(s) 505
BbsI GAAGAC 1 cut(s) 404
Bbv12I GWGCWC 1 cut(s) 318
BbvCI CCTCAGC 1 cut(s) 68
BbvI GCAGC 1 cut(s) 14
BccI CCATC 1 cut(s) 481
BceAI ACGGC 1 cut(s) 239
BcgI CGANNNNNNTGC 2 cut(s) 239, 273
BcoDI GTCTC 2 cut(s) 144, 372
BcuI ACTAGT 1 cut(s) 401
BfaI CTAG 1 cut(s) 402
BisI GCNGC 1 cut(s) 28
BlsI GCNGC 1 cut(s) 29
Bme18I GGWCC 1 cut(s) 505
BmgT120I GGNCC 1 cut(s) 505
BmiI GGNNCC 1 cut(s) 507
BmsI GCATC 4 cut(s) 48, 124, 303, 337
BpiI GAAGAC 1 cut(s) 404
Bpu10I CCTNAGC 1 cut(s) 68
Bpu14I TTCGAA 1 cut(s) 249
BsaBI GATNNNNATC 1 cut(s) 327
BsaWI WCCGGW 1 cut(s) 331
Bsc4I CCNNNNNNNGG 2 cut(s) 110, 307
Bse3DI GCAATG 1 cut(s) 60
Bse8I GATNNNNATC 1 cut(s) 327
BseGI GGATG 2 cut(s) 39, 328
BseJI GATNNNNATC 1 cut(s) 327
BseLI CCNNNNNNNGG 2 cut(s) 110, 307
BseMI GCAATG 1 cut(s) 60
BseMII CTCAG 1 cut(s) 82
BseXI GCAGC 1 cut(s) 14
Bsh1236I CGCG 1 cut(s) 93
BsiHKAI GWGCWC 1 cut(s) 318
BsiSI CCGG 1 cut(s) 332
BslFI GGGAC 1 cut(s) 491
BslI CCNNNNNNNGG 2 cut(s) 110, 307
BsmAI GTCTC 2 cut(s) 144, 372
BsmBI CGTCTC 1 cut(s) 144
BsmFI GGGAC 1 cut(s) 491
Bsp119I TTCGAA 1 cut(s) 249
Bsp1286I GDGCHC 1 cut(s) 318
Bsp143I GATC 1 cut(s) 114
Bsp68I TCGCGA 1 cut(s) 93
BspACI CCGC 1 cut(s) 191
BspCNI CTCAG 1 cut(s) 81
BspFNI CGCG 1 cut(s) 93
BspHI TCATGA 1 cut(s) 45
BspLI GGNNCC 1 cut(s) 507
BspPI GGATC 1 cut(s) 122
BspT104I TTCGAA 1 cut(s) 249
BsrDI GCAATG 1 cut(s) 60
BssMI GATC 1 cut(s) 114
Bst4CI ACNGT 2 cut(s) 278, 409
Bst6I CTCTTC 1 cut(s) 375
BstAPI GCANNNNNTGC 1 cut(s) 455
BstBI TTCGAA 1 cut(s) 249
BstC8I GCNNGC 2 cut(s) 7, 493
BstDEI CTNAG 3 cut(s) 68, 187, 567
BstF5I GGATG 2 cut(s) 39, 328
BstFNI CGCG 1 cut(s) 93
BstKTI GATC 1 cut(s) 117
BstMAI GTCTC 2 cut(s) 144, 372
BstMBI GATC 1 cut(s) 114
BstMWI GCNNNNNNNGC 3 cut(s) 36, 99, 455
BstNSI RCATGY 1 cut(s) 495
BstUI CGCG 1 cut(s) 93
BstV1I GCAGC 1 cut(s) 14
BstV2I GAAGAC 1 cut(s) 404
BtsCI GGATG 2 cut(s) 39, 328
BtuMI TCGCGA 1 cut(s) 93
Cac8I GCNNGC 2 cut(s) 7, 493
CciI TCATGA 1 cut(s) 45
Cfr13I GGNCC 1 cut(s) 505
CseI GACGC 2 cut(s) 61, 126
Csp6I GTAC 2 cut(s) 121, 405
CviAII CATG 2 cut(s) 46, 492
CviJI RGCY 4 cut(s) 5, 102, 254, 566
CviKI_1 RGCY 4 cut(s) 5, 102, 254, 566
CviQI GTAC 2 cut(s) 121, 405
DdeI CTNAG 3 cut(s) 68, 187, 567
DpnI GATC 1 cut(s) 116
DpnII GATC 1 cut(s) 114
DrdI GACNNNNNNGTC 1 cut(s) 136
DseDI GACNNNNNNGTC 1 cut(s) 136
Eam1104I CTCTTC 1 cut(s) 375
EarI CTCTTC 1 cut(s) 375
Eco32I GATATC 1 cut(s) 418
Eco47I GGWCC 1 cut(s) 505
EcoO109I RGGNCCY 1 cut(s) 505
EcoRI GAATTC 1 cut(s) 514
EcoRV GATATC 1 cut(s) 418
Esp3I CGTCTC 1 cut(s) 144
FaeI CATG 2 cut(s) 49, 495
FaiI YATR 5 cut(s) 47, 414, 456, 482, 493
FaqI GGGAC 1 cut(s) 491
FatI CATG 2 cut(s) 45, 491
Fnu4HI GCNGC 1 cut(s) 28
FokI GGATG 2 cut(s) 26, 315
Fsp4HI GCNGC 1 cut(s) 28
FspBI CTAG 1 cut(s) 402
GluI GCNGC 1 cut(s) 28
HapII CCGG 1 cut(s) 332
HgaI GACGC 2 cut(s) 61, 126
Hin1II CATG 2 cut(s) 49, 495
HinfI GANTC 1 cut(s) 469
HpaII CCGG 1 cut(s) 332
HphI GGTGA 2 cut(s) 139, 346
Hpy188I TCNGA 3 cut(s) 144, 229, 375
Hpy188III TCNNGA 5 cut(s) 46, 92, 196, 466, 473
Hpy99I CGWCG 1 cut(s) 77
HpyAV CCTTC 4 cut(s) 313, 427, 496, 556
HpyCH4III ACNGT 2 cut(s) 278, 409
HpyCH4IV ACGT 1 cut(s) 109
HpyCH4V TGCA 4 cut(s) 9, 39, 341, 495
HpyF10VI GCNNNNNNNGC 3 cut(s) 36, 99, 455
HpyF3I CTNAG 3 cut(s) 68, 187, 567
HpySE526I ACGT 1 cut(s) 109
Hsp92II CATG 2 cut(s) 49, 495
Kzo9I GATC 1 cut(s) 114
LmnI GCTCC 1 cut(s) 355
LpnPI CCDG 2 cut(s) 170, 345
Lsp1109I GCAGC 1 cut(s) 14
LweI GCATC 4 cut(s) 48, 124, 303, 337
MaeI CTAG 1 cut(s) 402
MaeII ACGT 1 cut(s) 109
MaeIII GTNAC 2 cut(s) 127, 193
MalI GATC 1 cut(s) 116
MboI GATC 1 cut(s) 114
MboII GAAGA 3 cut(s) 362, 409, 496
MfeI CAATTG 1 cut(s) 10
MhlI GDGCHC 1 cut(s) 318
MluCI AATT 6 cut(s) 10, 149, 179, 245, 514, 539
MmeI TCCRAC 1 cut(s) 207
MnlI CCTC 7 cut(s) 53, 77, 118, 185, 301, 376, 538
MseI TTAA 1 cut(s) 281
MslI CAYNNNNRTG 1 cut(s) 44
MspI CCGG 1 cut(s) 332
MunI CAATTG 1 cut(s) 10
MvnI CGCG 1 cut(s) 93
MwoI GCNNNNNNNGC 3 cut(s) 36, 99, 455
NdeII GATC 1 cut(s) 114
NlaIII CATG 2 cut(s) 49, 495
NlaIV GGNNCC 1 cut(s) 507
NmuCI GTSAC 2 cut(s) 127, 193
NruI TCGCGA 1 cut(s) 93
NspI RCATGY 1 cut(s) 495
NspV TTCGAA 1 cut(s) 249
PaeI GCATGC 1 cut(s) 495
PagI TCATGA 1 cut(s) 45
PcsI WCGNNNNNNNCGW 4 cut(s) 81, 90, 246, 303
PfeI GAWTC 1 cut(s) 469
PkrI GCNGC 1 cut(s) 29
PpuMI RGGWCCY 1 cut(s) 505
Psp5II RGGWCCY 1 cut(s) 505
PspN4I GGNNCC 1 cut(s) 507
PspPI GGNCC 1 cut(s) 505
PspPPI RGGWCCY 1 cut(s) 505
RruI TCGCGA 1 cut(s) 93
RsaI GTAC 2 cut(s) 122, 406
RsaNI GTAC 2 cut(s) 121, 405
RseI CAYNNNNRTG 1 cut(s) 44
SaqAI TTAA 1 cut(s) 281
SatI GCNGC 1 cut(s) 28
Sau3AI GATC 1 cut(s) 114
Sau96I GGNCC 1 cut(s) 505
SduI GDGCHC 1 cut(s) 318
SetI ASST 9 cut(s) 112, 129, 159, 227, 312, 387, 438, 507, 549
SfaNI GCATC 4 cut(s) 48, 124, 303, 337
SfuI TTCGAA 1 cut(s) 249
SinI GGWCC 1 cut(s) 505
SmiMI CAYNNNNRTG 1 cut(s) 44
SpeI ACTAGT 1 cut(s) 401
SphI GCATGC 1 cut(s) 495
Sse9I AATT 6 cut(s) 10, 149, 179, 245, 514, 539
SsiI CCGC 1 cut(s) 191
SspMI CTAG 1 cut(s) 402
TaaI ACNGT 2 cut(s) 278, 409
TaiI ACGT 1 cut(s) 112
TaqI TCGA 4 cut(s) 207, 249, 297, 306
TasI AATT 6 cut(s) 10, 149, 179, 245, 514, 539
TfiI GAWTC 1 cut(s) 469
Tru1I TTAA 1 cut(s) 281
Tru9I TTAA 1 cut(s) 281
TseFI GTSAC 2 cut(s) 127, 193
TseI GCWGC 1 cut(s) 27
Tsp45I GTSAC 2 cut(s) 127, 193
TspDTI ATGAA 3 cut(s) 46, 410, 497
TspGWI ACGGA 1 cut(s) 537
VpaK11BI GGWCC 1 cut(s) 505
XapI RAATTY 4 cut(s) 179, 245, 514, 539
XceI RCATGY 1 cut(s) 495
XspI CTAG 1 cut(s) 402
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.