FvH4_6g13012

Aldo-keto reductase family 4 member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0014906
Physical Location & Seq
Reverse (-)
7892966 .. 7895644
2679 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g13012.t1

Sequence Viewer

Length: 876 bp
ATGGCACACGAAATTCAATTCTTTGAGCTGAACACCGGAGCCAAGATCCCCTCGTTGGGCTTAGGGACCTGGCAATCCAGCCCTGGCGTCGTCGGCGACGCCGTCGCCACCGCCGTTAAGGCAGGGTACCGCCATATTGATTGTGCTCAACGCTATGGAAATGAGAAGGAGATTGGCCTAGTTCTGAAGAAGTTGTTTGAACATGGTGTGGTGAAGCGCGAGGAGTTGTGGATTACTTCCAAACTCTGGTGCACTGATCATGCCCCAGAGGATGTAACAGTGGCATTGGAAAAGACTTTGAGAGACTTGCAGCTGGACTATGTCGACTTGCACCTTATGCATTGGCCAGTACGGTTGAAGAAGGGTTCAGTAGATTTTAGTCCCGAAAACCTTATGCAACCAGATATCCCTGCCACATGGAGGGCAATGGAATCTCTCTTTGATCTGGGTAAGGCTCGAGCTATTGGTGTGAGCAATTTCTCTACAAAGAAGCTGTCAGATTTGCTGGATGTGGCACGTGTTCCTCCTGCTGTTAACCAAGTGGAGTGCCATCCTTCATGGCAGCAGGCCAAGCTACATTCATTCTGTAAATCCAATGGAGTTCATGTTTCTGGATATTCACCCCTTGGTTCTCCAGGCACAACATGGATCAAAAGTGACGTCCTTGAGAATCCTATTCTGAACATGGTTGCAGAGAAACTAGGCAAAACTGCTGCACAAGTTTCTCTTCGATGGGGACTGCAAAAGGGTCACAGTGTTCTTCCAAAGAGTACAAATGAAGCAAGGATCAAGCAGAACTTTGATGTCTTTGGGTGGTCTATCCCAGAGGACTTGTTCACCAAGCTTTCTCAAATTGGGCAGGCAAGTACTCAATGA

Protein Analysis

292

Amino Acids

32.28

Weight (kDa)

7.73

Isoelectric Point (pI)

34.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldo_ket_red PF00248 19 - 285 1.8e-50 Aldo/keto reductase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014906)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G37770 AT2G37790 AT3G53880 AT3G53880
fragaria_vesca FvH4_6g13012 FvH4_6g13012
malus_domestica MD12G1150400.v1.1
prunus_persica Prupe.6G262000_v2.0.a1
pyrus_communis pycom12g14420
rosa_chinensis RchiOBHm_Chr3g0465141
rosa_multiflora Rmu_sc0003386.1_g000011
rosa_roxburghii Rroxscaffold_6G00415210
rosa_rugosa Rorug03G0075700
rosa_samantha Rh3BG139400 Rh3DG140700
rosa_wichuraiana Rw3G011410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 663
Acc65I GGTACC 1 cut(s) 126
AccB1I GGYRCC 1 cut(s) 126
AccB7I CCANNNNNTGG 1 cut(s) 246
AccI GTMKAC 1 cut(s) 324
AccII CGCG 1 cut(s) 219
AciI CCGC 2 cut(s) 111, 130
AclWI GGATC 3 cut(s) 40, 656, 794
AcoI YGGCCR 1 cut(s) 344
AcsI RAATTY 1 cut(s) 12
AcuI CTGAAG 1 cut(s) 206
AcvI CACGTG 1 cut(s) 518
AcyI GRCGYC 3 cut(s) 87, 99, 660
AfaI GTAC 4 cut(s) 128, 351, 772, 868
AfiI CCNNNNNNNGG 4 cut(s) 55, 56, 246, 420
AflIII ACRYGT 1 cut(s) 517
AgsI TTSAA 3 cut(s) 17, 200, 358
AjnI CCWGG 3 cut(s) 68, 82, 634
AluBI AGCT 6 cut(s) 28, 313, 461, 493, 574, 844
AluI AGCT 6 cut(s) 28, 313, 461, 493, 574, 844
Alw21I GWGCWC 2 cut(s) 148, 254
Alw26I GTCTC 1 cut(s) 297
Alw44I GTGCAC 1 cut(s) 250
AlwI GGATC 3 cut(s) 40, 656, 794
Ama87I CYCGRG 1 cut(s) 456
AoxI GGCC 3 cut(s) 175, 344, 567
ApaLI GTGCAC 1 cut(s) 250
ApeKI GCWGC 3 cut(s) 310, 562, 713
ApoI RAATTY 1 cut(s) 12
ArsI GACNNNNNNTTYG 4 cut(s) 479, 511, 645, 677
Asp718I GGTACC 1 cut(s) 126
AspLEI GCGC 1 cut(s) 219
AspS9I GGNCC 1 cut(s) 66
AsuHPI GGTGA 3 cut(s) 223, 612, 829
AvaI CYCGRG 1 cut(s) 456
AvaII GGWCC 1 cut(s) 66
BaeGI GKGCMC 1 cut(s) 254
BalI TGGCCA 1 cut(s) 346
BanI GGYRCC 1 cut(s) 126
BbrPI CACGTG 1 cut(s) 518
Bbv12I GWGCWC 2 cut(s) 148, 254
BbvI GCAGC 3 cut(s) 322, 574, 700
BccI CCATC 2 cut(s) 558, 726
BceAI ACGGC 2 cut(s) 86, 98
BciT130I CCWGG 3 cut(s) 70, 84, 636
BclI TGATCA 1 cut(s) 256
BcoDI GTCTC 1 cut(s) 297
BfaI CTAG 2 cut(s) 179, 701
BglI GCCNNNNNGGC 1 cut(s) 119
BisI GCNGC 3 cut(s) 311, 563, 714
BlsI GCNGC 3 cut(s) 312, 564, 715
BmcAI AGTACT 1 cut(s) 868
Bme1390I CCNGG 3 cut(s) 70, 84, 636
Bme18I GGWCC 1 cut(s) 66
BmeT110I CYCGRG 1 cut(s) 456
BmgT120I GGNCC 1 cut(s) 66
BmiI GGNNCC 3 cut(s) 40, 67, 128
BmrFI CCNGG 3 cut(s) 70, 84, 636
BpmI CTGGAG 1 cut(s) 618
Bpu10I CCTNAGC 1 cut(s) 61
BpuEI CTTGAG 1 cut(s) 686
BsaAI YACGTR 1 cut(s) 518
BsaHI GRCGYC 3 cut(s) 87, 99, 660
BsaJI CCNNGG 2 cut(s) 82, 625
BsaWI WCCGGW 1 cut(s) 35
BsaXI ACNNNNNCTCC 2 cut(s) 591, 621
Bsc4I CCNNNNNNNGG 4 cut(s) 55, 56, 246, 420
Bse1I ACTGG 1 cut(s) 347
Bse3DI GCAATG 1 cut(s) 432
BseBI CCWGG 3 cut(s) 70, 84, 636
BseDI CCNNGG 2 cut(s) 82, 625
BseGI GGATG 3 cut(s) 277, 514, 550
BseLI CCNNNNNNNGG 4 cut(s) 55, 56, 246, 420
BseMI GCAATG 1 cut(s) 432
BseNI ACTGG 1 cut(s) 347
BseRI GAGGAG 1 cut(s) 236
BseSI GKGCMC 1 cut(s) 254
BseXI GCAGC 3 cut(s) 322, 574, 700
BsgI GTGCAG 1 cut(s) 699
Bsh1236I CGCG 1 cut(s) 219
BshFI GGCC 3 cut(s) 177, 346, 569
BshNI GGYRCC 1 cut(s) 126
BsiHKAI GWGCWC 2 cut(s) 148, 254
BsiHKCI CYCGRG 1 cut(s) 456
BsiSI CCGG 1 cut(s) 36
BslFI GGGAC 3 cut(s) 79, 366, 750
BslI CCNNNNNNNGG 4 cut(s) 55, 56, 246, 420
BsmAI GTCTC 1 cut(s) 297
BsmFI GGGAC 3 cut(s) 79, 366, 750
BsnI GGCC 3 cut(s) 177, 346, 569
BsoBI CYCGRG 1 cut(s) 456
Bsp1286I GDGCHC 2 cut(s) 148, 254
Bsp143I GATC 5 cut(s) 45, 256, 442, 648, 786
BspACI CCGC 2 cut(s) 111, 130
BspANI GGCC 3 cut(s) 177, 346, 569
BspFNI CGCG 1 cut(s) 219
BspLI GGNNCC 3 cut(s) 40, 67, 128
BspPI GGATC 3 cut(s) 40, 656, 794
BspT107I GGYRCC 1 cut(s) 126
BsrDI GCAATG 1 cut(s) 432
BsrI ACTGG 1 cut(s) 347
BssECI CCNNGG 2 cut(s) 82, 625
BssMI GATC 5 cut(s) 45, 256, 442, 648, 786
BssNI GRCGYC 3 cut(s) 87, 99, 660
BssT1I CCWWGG 1 cut(s) 625
Bst2UI CCWGG 3 cut(s) 70, 84, 636
Bst4CI ACNGT 3 cut(s) 280, 354, 755
Bst6I CTCTTC 1 cut(s) 732
BstACI GRCGYC 3 cut(s) 87, 99, 660
BstAPI GCANNNNNTGC 1 cut(s) 337
BstBAI YACGTR 1 cut(s) 518
BstC8I GCNNGC 2 cut(s) 567, 861
BstDEI CTNAG 1 cut(s) 61
BstF5I GGATG 3 cut(s) 277, 514, 550
BstFNI CGCG 1 cut(s) 219
BstHHI GCGC 1 cut(s) 219
BstKTI GATC 5 cut(s) 48, 259, 445, 651, 789
BstMAI GTCTC 1 cut(s) 297
BstMBI GATC 5 cut(s) 45, 256, 442, 648, 786
BstMWI GCNNNNNNNGC 4 cut(s) 93, 119, 337, 571
BstNI CCWGG 3 cut(s) 70, 84, 636
BstSCI CCNGG 3 cut(s) 68, 82, 634
BstSLI GKGCMC 1 cut(s) 254
BstUI CGCG 1 cut(s) 219
BstV1I GCAGC 3 cut(s) 322, 574, 700
BstX2I RGATCY 1 cut(s) 45
BstYI RGATCY 1 cut(s) 45
BsuRI GGCC 3 cut(s) 177, 346, 569
BtsCI GGATG 3 cut(s) 277, 514, 550
BtsIMutI CAGTG 3 cut(s) 252, 285, 760
Cac8I GCNNGC 2 cut(s) 567, 861
CfoI GCGC 1 cut(s) 219
Cfr13I GGNCC 1 cut(s) 66
CseI GACGC 2 cut(s) 76, 107
Csp6I GTAC 4 cut(s) 127, 350, 771, 867
CviAII CATG 7 cut(s) 203, 260, 417, 558, 605, 645, 685
CviQI GTAC 4 cut(s) 127, 350, 771, 867
DdeI CTNAG 1 cut(s) 61
DpnI GATC 5 cut(s) 47, 258, 444, 650, 788
DpnII GATC 5 cut(s) 45, 256, 442, 648, 786
EaeI YGGCCR 1 cut(s) 344
Eam1104I CTCTTC 1 cut(s) 732
EarI CTCTTC 1 cut(s) 732
Eco130I CCWWGG 1 cut(s) 625
Eco32I GATATC 1 cut(s) 406
Eco47I GGWCC 1 cut(s) 66
Eco57I CTGAAG 1 cut(s) 206
Eco72I CACGTG 1 cut(s) 518
Eco88I CYCGRG 1 cut(s) 456
EcoO109I RGGNCCY 1 cut(s) 66
EcoRII CCWGG 3 cut(s) 68, 82, 634
EcoRV GATATC 1 cut(s) 406
EcoT14I CCWWGG 1 cut(s) 625
EcoT22I ATGCAT 1 cut(s) 342
ErhI CCWWGG 1 cut(s) 625
FaeI CATG 7 cut(s) 206, 263, 420, 561, 608, 648, 688
FalI AAGNNNNNCTT 4 cut(s) 711, 743, 782, 814
FaqI GGGAC 3 cut(s) 79, 366, 750
FatI CATG 7 cut(s) 202, 259, 416, 557, 604, 644, 684
FbaI TGATCA 1 cut(s) 256
FblI GTMKAC 1 cut(s) 324
Fnu4HI GCNGC 3 cut(s) 311, 563, 714
FokI GGATG 3 cut(s) 284, 521, 537
Fsp4HI GCNGC 3 cut(s) 311, 563, 714
FspBI CTAG 2 cut(s) 179, 701
GlaI GCGC 1 cut(s) 218
GluI GCNGC 3 cut(s) 311, 563, 714
GsuI CTGGAG 1 cut(s) 618
HaeIII GGCC 3 cut(s) 177, 346, 569
HapII CCGG 1 cut(s) 36
HgaI GACGC 2 cut(s) 76, 107
HhaI GCGC 1 cut(s) 219
Hin1I GRCGYC 3 cut(s) 87, 99, 660
Hin1II CATG 7 cut(s) 206, 263, 420, 561, 608, 648, 688
Hin6I GCGC 1 cut(s) 217
HinP1I GCGC 1 cut(s) 217
HincII GTYRAC 2 cut(s) 325, 535
HindII GTYRAC 2 cut(s) 325, 535
HindIII AAGCTT 1 cut(s) 842
HinfI GANTC 2 cut(s) 431, 670
HpaI GTTAAC 1 cut(s) 535
HpaII CCGG 1 cut(s) 36
HphI GGTGA 3 cut(s) 223, 612, 829
Hpy166II GTNNAC 4 cut(s) 252, 325, 535, 837
Hpy188I TCNGA 3 cut(s) 186, 499, 681
Hpy188III TCNNGA 2 cut(s) 383, 612
Hpy8I GTNNAC 4 cut(s) 252, 325, 535, 837
Hpy99I CGWCG 4 cut(s) 92, 95, 101, 107
HpyAV CCTTC 3 cut(s) 160, 355, 564
HpyCH4III ACNGT 3 cut(s) 280, 354, 755
HpyCH4IV ACGT 2 cut(s) 517, 660
HpyCH4V TGCA 8 cut(s) 252, 310, 331, 340, 397, 692, 716, 742
HpyF10VI GCNNNNNNNGC 4 cut(s) 93, 119, 337, 571
HpyF3I CTNAG 1 cut(s) 61
HpySE526I ACGT 2 cut(s) 517, 660
Hsp92I GRCGYC 3 cut(s) 87, 99, 660
Hsp92II CATG 7 cut(s) 206, 263, 420, 561, 608, 648, 688
HspAI GCGC 1 cut(s) 217
KpnI GGTACC 1 cut(s) 130
Ksp22I TGATCA 1 cut(s) 256
KspAI GTTAAC 1 cut(s) 535
Kzo9I GATC 5 cut(s) 45, 256, 442, 648, 786
LmnI GCTCC 1 cut(s) 38
Lsp1109I GCAGC 3 cut(s) 322, 574, 700
MaeI CTAG 2 cut(s) 179, 701
MaeII ACGT 2 cut(s) 517, 660
MaeIII GTNAC 3 cut(s) 274, 656, 749
MalI GATC 5 cut(s) 47, 258, 444, 650, 788
MboI GATC 5 cut(s) 45, 256, 442, 648, 786
MboII GAAGA 4 cut(s) 199, 370, 719, 752
MflI RGATCY 1 cut(s) 45
MhlI GDGCHC 2 cut(s) 148, 254
MlsI TGGCCA 1 cut(s) 346
MluCI AATT 4 cut(s) 12, 17, 475, 852
MluNI TGGCCA 1 cut(s) 346
MnlI CCTC 6 cut(s) 61, 214, 262, 414, 534, 820
Mox20I TGGCCA 1 cut(s) 346
Mph1103I ATGCAT 1 cut(s) 342
MscI TGGCCA 1 cut(s) 346
MseI TTAA 2 cut(s) 117, 534
Msp20I TGGCCA 1 cut(s) 346
MspA1I CMGCKG 1 cut(s) 313
MspI CCGG 1 cut(s) 36
MspR9I CCNGG 3 cut(s) 70, 84, 636
MvaI CCWGG 3 cut(s) 70, 84, 636
MvnI CGCG 1 cut(s) 219
MwoI GCNNNNNNNGC 4 cut(s) 93, 119, 337, 571
NdeII GATC 5 cut(s) 45, 256, 442, 648, 786
NlaIII CATG 7 cut(s) 206, 263, 420, 561, 608, 648, 688
NlaIV GGNNCC 3 cut(s) 40, 67, 128
NmuCI GTSAC 2 cut(s) 656, 749
NsiI ATGCAT 1 cut(s) 342
PaeR7I CTCGAG 1 cut(s) 456
PcsI WCGNNNNNNNCGW 2 cut(s) 99, 111
PfeI GAWTC 2 cut(s) 431, 670
PflFI GACNNNGTC 2 cut(s) 101, 320
PflMI CCANNNNNTGG 1 cut(s) 246
PkrI GCNGC 3 cut(s) 312, 564, 715
PmaCI CACGTG 1 cut(s) 518
PmlI CACGTG 1 cut(s) 518
Ppu21I YACGTR 1 cut(s) 518
PpuMI RGGWCCY 1 cut(s) 66
Psp5II RGGWCCY 1 cut(s) 66
Psp6I CCWGG 3 cut(s) 68, 82, 634
PspCI CACGTG 1 cut(s) 518
PspGI CCWGG 3 cut(s) 68, 82, 634
PspN4I GGNNCC 3 cut(s) 40, 67, 128
PspPI GGNCC 1 cut(s) 66
PspPPI RGGWCCY 1 cut(s) 66
PspXI VCTCGAGB 1 cut(s) 456
PsuI RGATCY 1 cut(s) 45
PsyI GACNNNGTC 2 cut(s) 101, 320
PvuII CAGCTG 1 cut(s) 313
RsaI GTAC 4 cut(s) 128, 351, 772, 868
RsaNI GTAC 4 cut(s) 127, 350, 771, 867
SalI GTCGAC 1 cut(s) 323
SaqAI TTAA 2 cut(s) 117, 534
SatI GCNGC 3 cut(s) 311, 563, 714
Sau3AI GATC 5 cut(s) 45, 256, 442, 648, 786
Sau96I GGNCC 1 cut(s) 66
ScaI AGTACT 1 cut(s) 868
ScrFI CCNGG 3 cut(s) 70, 84, 636
SduI GDGCHC 2 cut(s) 148, 254
Sfr274I CTCGAG 1 cut(s) 456
SinI GGWCC 1 cut(s) 66
SlaI CTCGAG 1 cut(s) 456
SmlI CTYRAG 2 cut(s) 456, 665
SmoI CTYRAG 2 cut(s) 456, 665
Sse9I AATT 4 cut(s) 12, 17, 475, 852
SsiI CCGC 2 cut(s) 111, 130
SspMI CTAG 2 cut(s) 179, 701
StyD4I CCNGG 3 cut(s) 68, 82, 634
StyI CCWWGG 1 cut(s) 625
TaaI ACNGT 3 cut(s) 280, 354, 755
TaiI ACGT 2 cut(s) 520, 663
TaqI TCGA 3 cut(s) 324, 457, 730
TasI AATT 4 cut(s) 12, 17, 475, 852
TatI WGTACW 2 cut(s) 770, 866
TfiI GAWTC 2 cut(s) 431, 670
Tru1I TTAA 2 cut(s) 117, 534
Tru9I TTAA 2 cut(s) 117, 534
TscAI CASTG 3 cut(s) 259, 285, 760
TseFI GTSAC 2 cut(s) 656, 749
TseI GCWGC 3 cut(s) 310, 562, 713
Tsp45I GTSAC 2 cut(s) 656, 749
TspDTI ATGAA 4 cut(s) 546, 570, 593, 792
TspRI CASTG 3 cut(s) 259, 285, 760
Tth111I GACNNNGTC 2 cut(s) 101, 320
Van91I CCANNNNNTGG 1 cut(s) 246
VneI GTGCAC 1 cut(s) 250
VpaK11BI GGWCC 1 cut(s) 66
XapI RAATTY 1 cut(s) 12
XcmI CCANNNNNNNNNTGG 1 cut(s) 642
XhoI CTCGAG 1 cut(s) 456
XmiI GTMKAC 1 cut(s) 324
XspI CTAG 2 cut(s) 179, 701
ZraI GACGTC 1 cut(s) 661
ZrmI AGTACT 1 cut(s) 868
Zsp2I ATGCAT 1 cut(s) 342
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.