Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0014906

Physical Location & Seq

Genomic Coordinates Forward (+)

23054870 .. 23057536

Gene Structure 2667 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1150400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 948 bp

ATGGCGGACGATATTCGTTTCTTCCGGTTGAACACCGGATCTAAGATCCCTTCAGTAGGGTTAGGGACCTGGCAGTCGAGCCCTGGCCTCGTCGGAGACGCCGTGGCCGCCGCCATCAAGGCAGGATATCGCCATATCGATTGTGCTCAACGCTATGGCAATGAGAAGGAGATTGGTTTGGTTCTCAAGAAGTTGTTTGAAGATGGCATGGTGAAGCGCGAGGATTTGTTTATTACCTCCAAACTCTGGAGTACAGATCATGCACCAGAAGATGTATTAGTGGCAATGGACAGGACTTTGAGAGACTTGCAGCTTGATTATGTTGACTTGTATCTGATGCATTGGCCAGTCCGGTTGAAGAAGGGGTCAGCAAGTTTTGATCCTGAAAACCTGGTTCAACCAGATATCCCTTGCACTTGGAGAGCAATGGAAACCCTCTATGATTCGGGTAAGGCTCGAGCTATTGGCGTAAGCAATTTCTCTACGAAGAAACTGTCAGATTTGCTGGATGTGGCACATATTCCTCCAGCTGCTGTCCAAGTGGAGTGTCATCCTTCGTGGCAGCAAGGCAAGCTACATTCTTTCTGTAAATCCAAGGGAGTTCATTTACAGGGATACTCACCACTGGGTTCTCCGGGCACATCATGGATCAAAGGTGACGTCCTTCAGAATCCAATCCTTCTCATGGTCGCAGAAAAACTAGGCAAAACTCCTGCACAAGTTGCTCTTAGGTGGGGACTGCAAATGGGGCACAGTGTTCTTCCTAAGAGTACTAATGAAACAAGGATCAAGGAGAACTTCAATGTCTTTGGCTGGTCTATCCCGGAGGACTTGTTCGCCAAGCTTTCTGAGATTCAGCAGGCAAGATTGGTTAGAGGGACTCAATTTGTTCATGATACGTATGGGGCCTACAGGACGCTTGAGGAATTGTGGGACGGAGAAATTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0001101 GO:0003674 GO:0003824 GO:0004033 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006950 GO:0006970 GO:0008106 GO:0008150 GO:0008152 GO:0009266 GO:0009409 GO:0009414 GO:0009415 GO:0009507 GO:0009536 GO:0009628 GO:0009651 GO:0010035 GO:0016229 GO:0016491 GO:0016614 GO:0016616 GO:0036094 GO:0042221 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0048037 GO:0050661 GO:0050662 GO:0050896 GO:0055114 GO:0070401 GO:0097159 GO:1901265 GO:1901363 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko00010 ko00040 ko00561 ko00930 ko01100 ko01110 ko01120 ko01130 ko01220 map00010 map00040 map00561 map00930 map01100 map01110 map01120 map01130 map01220

Pfam Domains

Protein Families

Aldo_ket_red

Protein Analysis

316

Amino Acids

35.22

Weight (kDa)

6.76

Isoelectric Point (pI)

35.23

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Aldo_ket_red	PF00248	19 - 285	7.9e-52	Aldo/keto reductase family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014906)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G37770 AT2G37790 AT3G53880 AT3G53880
fragaria_vesca	FvH4_6g13012 FvH4_6g13012
malus_domestica	MD12G1150400.v1.1
prunus_persica	Prupe.6G262000_v2.0.a1
pyrus_communis	pycom12g14420
rosa_chinensis	RchiOBHm_Chr3g0465141
rosa_multiflora	Rmu_sc0003386.1_g000011
rosa_roxburghii	Rroxscaffold_6G00415210
rosa_rugosa	Rorug03G0075700
rosa_samantha	Rh3BG139400 Rh3DG140700
rosa_wichuraiana	Rw3G011410

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	73
AatII	GACGTC	1 cut(s)	663
AccB7I	CCANNNNNTGG	1 cut(s)	246
AccII	CGCG	1 cut(s)	219
AciI	CCGC	3 cut(s)	5, 108, 111
AclWI	GGATC	5 cut(s)	40, 46, 374, 656, 794
AcoI	YGGCCR	2 cut(s)	105, 344
AcsI	RAATTY	1 cut(s)	942
AcuI	CTGAAG	2 cut(s)	36, 650
AcyI	GRCGYC	2 cut(s)	99, 660
AfaI	GTAC	2 cut(s)	253, 772
AfiI	CCNNNNNNNGG	3 cut(s)	56, 246, 685
AgsI	TTSAA	5 cut(s)	31, 200, 358, 398, 802
AjnI	CCWGG	3 cut(s)	68, 82, 390
AloI	GAACNNNNNNTCC	2 cut(s)	818, 850
AluBI	AGCT	5 cut(s)	313, 461, 530, 574, 844
AluI	AGCT	5 cut(s)	313, 461, 530, 574, 844
Alw21I	GWGCWC	1 cut(s)	148
Alw26I	GTCTC	2 cut(s)	90, 297
AlwI	GGATC	5 cut(s)	40, 46, 374, 656, 794
AlwNI	CAGNNNCTG	1 cut(s)	533
Ama87I	CYCGRG	1 cut(s)	456
AoxI	GGCC	4 cut(s)	85, 105, 344, 906
ApeKI	GCWGC	3 cut(s)	310, 530, 562
ApoI	RAATTY	1 cut(s)	942
ArsI	GACNNNNNNTTYG	4 cut(s)	479, 511, 645, 677
AspLEI	GCGC	1 cut(s)	219
AspS9I	GGNCC	2 cut(s)	66, 906
AsuC2I	CCSGG	2 cut(s)	636, 824
AsuHPI	GGTGA	3 cut(s)	223, 612, 668
AvaI	CYCGRG	1 cut(s)	456
AvaII	GGWCC	1 cut(s)	66
BaeGI	GKGCMC	2 cut(s)	641, 753
BalI	TGGCCA	1 cut(s)	346
BanII	GRGCYC	1 cut(s)	83
Bbv12I	GWGCWC	1 cut(s)	148
BbvI	GCAGC	3 cut(s)	322, 517, 574
BccI	CCATC	2 cut(s)	122, 197
BceAI	ACGGC	1 cut(s)	86
BciT130I	CCWGG	3 cut(s)	70, 84, 392
BciVI	GTATCC	1 cut(s)	608
BcnI	CCSGG	2 cut(s)	636, 824
BcoDI	GTCTC	2 cut(s)	90, 297
BfaI	CTAG	1 cut(s)	701
BfmI	CTRYAG	1 cut(s)	910
BfuI	GTATCC	1 cut(s)	608
BglI	GCCNNNNNGGC	1 cut(s)	119
BisI	GCNGC	5 cut(s)	108, 111, 311, 531, 563
BlsI	GCNGC	5 cut(s)	109, 112, 312, 532, 564
BmcAI	AGTACT	1 cut(s)	772
Bme1390I	CCNGG	5 cut(s)	70, 84, 392, 636, 824
Bme18I	GGWCC	1 cut(s)	66
BmeT110I	CYCGRG	1 cut(s)	456
BmgT120I	GGNCC	2 cut(s)	66, 906
BmiI	GGNNCC	2 cut(s)	67, 907
BmrFI	CCNGG	5 cut(s)	70, 84, 392, 636, 824
BmrI	ACTGGG	1 cut(s)	635
BmsI	GCATC	1 cut(s)	327
BmuI	ACTGGG	1 cut(s)	635
BpmI	CTGGAG	2 cut(s)	268, 510
BpuEI	CTTGAG	2 cut(s)	170, 941
BpuMI	CCSGG	2 cut(s)	636, 824
Bsa29I	ATCGAT	1 cut(s)	138
BsaAI	YACGTR	1 cut(s)	900
BsaHI	GRCGYC	2 cut(s)	99, 660
BsaJI	CCNNGG	3 cut(s)	82, 102, 594
BsaWI	WCCGGW	3 cut(s)	24, 35, 351
BsaXI	ACNNNNNCTCC	4 cut(s)	87, 117, 818, 848
Bsc4I	CCNNNNNNNGG	3 cut(s)	56, 246, 685
Bse1I	ACTGG	2 cut(s)	347, 630
Bse3DI	GCAATG	3 cut(s)	166, 291, 432
BseBI	CCWGG	3 cut(s)	70, 84, 392
BseCI	ATCGAT	1 cut(s)	138
BseDI	CCNNGG	3 cut(s)	82, 102, 594
BseGI	GGATG	2 cut(s)	514, 550
BseLI	CCNNNNNNNGG	3 cut(s)	56, 246, 685
BseMI	GCAATG	3 cut(s)	166, 291, 432
BseMII	CTCAG	1 cut(s)	840
BseNI	ACTGG	2 cut(s)	347, 630
BseSI	GKGCMC	2 cut(s)	641, 753
BseXI	GCAGC	3 cut(s)	322, 517, 574
BsgI	GTGCAG	1 cut(s)	699
Bsh1236I	CGCG	1 cut(s)	219
BshFI	GGCC	4 cut(s)	87, 107, 346, 908
BshVI	ATCGAT	1 cut(s)	138
BsiHKAI	GWGCWC	1 cut(s)	148
BsiHKCI	CYCGRG	1 cut(s)	456
BsiSI	CCGG	5 cut(s)	25, 36, 352, 635, 824
BslFI	GGGAC	3 cut(s)	79, 750, 892
BslI	CCNNNNNNNGG	3 cut(s)	56, 246, 685
BsmAI	GTCTC	2 cut(s)	90, 297
BsmBI	CGTCTC	1 cut(s)	90
BsmFI	GGGAC	3 cut(s)	79, 750, 892
BsnI	GGCC	4 cut(s)	87, 107, 346, 908
BsoBI	CYCGRG	1 cut(s)	456
Bsp1286I	GDGCHC	4 cut(s)	83, 148, 641, 753
Bsp143I	GATC	6 cut(s)	38, 45, 256, 379, 648, 786
BspACI	CCGC	3 cut(s)	5, 108, 111
BspANI	GGCC	4 cut(s)	87, 107, 346, 908
BspCNI	CTCAG	1 cut(s)	841
BspDI	ATCGAT	1 cut(s)	138
BspFNI	CGCG	1 cut(s)	219
BspHI	TCATGA	1 cut(s)	892
BspLI	GGNNCC	2 cut(s)	67, 907
BspPI	GGATC	5 cut(s)	40, 46, 374, 656, 794
BsrDI	GCAATG	3 cut(s)	166, 291, 432
BsrI	ACTGG	2 cut(s)	347, 630
BssECI	CCNNGG	3 cut(s)	82, 102, 594
BssMI	GATC	6 cut(s)	38, 45, 256, 379, 648, 786
BssNI	GRCGYC	2 cut(s)	99, 660
BssT1I	CCWWGG	1 cut(s)	594
Bst2UI	CCWGG	3 cut(s)	70, 84, 392
Bst4CI	ACNGT	2 cut(s)	495, 755
BstACI	GRCGYC	2 cut(s)	99, 660
BstAPI	GCANNNNNTGC	1 cut(s)	722
BstBAI	YACGTR	1 cut(s)	900
BstC8I	GCNNGC	2 cut(s)	572, 861
BstDEI	CTNAG	4 cut(s)	42, 728, 765, 849
BstDSI	CCRYGG	1 cut(s)	102
BstENI	CCTNNNNNAGG	1 cut(s)	54
BstF5I	GGATG	2 cut(s)	514, 550
BstFNI	CGCG	1 cut(s)	219
BstHHI	GCGC	1 cut(s)	219
BstKTI	GATC	6 cut(s)	41, 48, 259, 382, 651, 789
BstMAI	GTCTC	2 cut(s)	90, 297
BstMBI	GATC	6 cut(s)	38, 45, 256, 379, 648, 786
BstMWI	GCNNNNNNNGC	5 cut(s)	107, 119, 571, 722, 748
BstNI	CCWGG	3 cut(s)	70, 84, 392
BstSCI	CCNGG	5 cut(s)	68, 82, 390, 634, 822
BstSFI	CTRYAG	1 cut(s)	910
BstSLI	GKGCMC	2 cut(s)	641, 753
BstSNI	TACGTA	1 cut(s)	900
BstUI	CGCG	1 cut(s)	219
BstV1I	GCAGC	3 cut(s)	322, 517, 574
BstX2I	RGATCY	2 cut(s)	38, 45
BstYI	RGATCY	2 cut(s)	38, 45
Bsu15I	ATCGAT	1 cut(s)	138
BsuI	GTATCC	1 cut(s)	608
BsuRI	GGCC	4 cut(s)	87, 107, 346, 908
BsuTUI	ATCGAT	1 cut(s)	138
BtgI	CCRYGG	1 cut(s)	102
BtsCI	GGATG	2 cut(s)	514, 550
BtsIMutI	CAGTG	2 cut(s)	623, 760
Cac8I	GCNNGC	2 cut(s)	572, 861
CaiI	CAGNNNCTG	1 cut(s)	533
CciI	TCATGA	1 cut(s)	892
CfoI	GCGC	1 cut(s)	219
Cfr13I	GGNCC	2 cut(s)	66, 906
ClaI	ATCGAT	1 cut(s)	138
CseI	GACGC	2 cut(s)	107, 925
CsiI	ACCWGGT	1 cut(s)	390
Csp6I	GTAC	2 cut(s)	252, 771
CviAII	CATG	5 cut(s)	208, 260, 645, 685, 893
CviQI	GTAC	2 cut(s)	252, 771
DdeI	CTNAG	4 cut(s)	42, 728, 765, 849
DpnI	GATC	6 cut(s)	40, 47, 258, 381, 650, 788
DpnII	GATC	6 cut(s)	38, 45, 256, 379, 648, 786
DrdI	GACNNNNNNGTC	1 cut(s)	73
DseDI	GACNNNNNNGTC	1 cut(s)	73
EaeI	YGGCCR	2 cut(s)	105, 344
EciI	GGCGGA	1 cut(s)	20
Eco105I	TACGTA	1 cut(s)	900
Eco130I	CCWWGG	1 cut(s)	594
Eco24I	GRGCYC	1 cut(s)	83
Eco32I	GATATC	2 cut(s)	128, 406
Eco47I	GGWCC	1 cut(s)	66
Eco57I	CTGAAG	2 cut(s)	36, 650
Eco88I	CYCGRG	1 cut(s)	456
EcoNI	CCTNNNNNAGG	1 cut(s)	54
EcoO109I	RGGNCCY	2 cut(s)	66, 906
EcoRII	CCWGG	3 cut(s)	68, 82, 390
EcoRV	GATATC	2 cut(s)	128, 406
EcoT14I	CCWWGG	1 cut(s)	594
EcoT22I	ATGCAT	1 cut(s)	342
EcoT38I	GRGCYC	1 cut(s)	83
ErhI	CCWWGG	1 cut(s)	594
Esp3I	CGTCTC	1 cut(s)	90
FaeI	CATG	5 cut(s)	211, 263, 648, 688, 896
FalI	AAGNNNNNCTT	4 cut(s)	711, 743, 782, 814
FaqI	GGGAC	3 cut(s)	79, 750, 892
FatI	CATG	5 cut(s)	207, 259, 644, 684, 892
Fnu4HI	GCNGC	5 cut(s)	108, 111, 311, 531, 563
FokI	GGATG	2 cut(s)	521, 537
FriOI	GRGCYC	1 cut(s)	83
Fsp4HI	GCNGC	5 cut(s)	108, 111, 311, 531, 563
FspBI	CTAG	1 cut(s)	701
GlaI	GCGC	1 cut(s)	218
GluI	GCNGC	5 cut(s)	108, 111, 311, 531, 563
GsuI	CTGGAG	2 cut(s)	268, 510
HaeIII	GGCC	4 cut(s)	87, 107, 346, 908
HapII	CCGG	5 cut(s)	25, 36, 352, 635, 824
HgaI	GACGC	2 cut(s)	107, 925
HhaI	GCGC	1 cut(s)	219
Hin1I	GRCGYC	2 cut(s)	99, 660
Hin1II	CATG	5 cut(s)	211, 263, 648, 688, 896
Hin6I	GCGC	1 cut(s)	217
HinP1I	GCGC	1 cut(s)	217
HincII	GTYRAC	1 cut(s)	325
HindII	GTYRAC	1 cut(s)	325
HindIII	AAGCTT	1 cut(s)	842
HinfI	GANTC	4 cut(s)	443, 670, 853, 880
HpaII	CCGG	5 cut(s)	25, 36, 352, 635, 824
HphI	GGTGA	3 cut(s)	223, 612, 668
Hpy166II	GTNNAC	1 cut(s)	325
Hpy188I	TCNGA	5 cut(s)	95, 336, 499, 669, 850
Hpy188III	TCNNGA	4 cut(s)	187, 247, 383, 893
Hpy8I	GTNNAC	1 cut(s)	325
Hpy99I	CGWCG	1 cut(s)	95
HpyAV	CCTTC	6 cut(s)	60, 160, 355, 564, 674, 689
HpyCH4III	ACNGT	2 cut(s)	495, 755
HpyCH4IV	ACGT	2 cut(s)	660, 899
HpyCH4V	TGCA	6 cut(s)	263, 310, 340, 414, 716, 742
HpyF10VI	GCNNNNNNNGC	5 cut(s)	107, 119, 571, 722, 748
HpyF3I	CTNAG	4 cut(s)	42, 728, 765, 849
HpySE526I	ACGT	2 cut(s)	660, 899
Hsp92I	GRCGYC	2 cut(s)	99, 660
Hsp92II	CATG	5 cut(s)	211, 263, 648, 688, 896
HspAI	GCGC	1 cut(s)	217
Kzo9I	GATC	6 cut(s)	38, 45, 256, 379, 648, 786
Lsp1109I	GCAGC	3 cut(s)	322, 517, 574
LweI	GCATC	1 cut(s)	327
MabI	ACCWGGT	1 cut(s)	390
MaeI	CTAG	1 cut(s)	701
MaeII	ACGT	2 cut(s)	660, 899
MaeIII	GTNAC	1 cut(s)	656
MalI	GATC	6 cut(s)	40, 47, 258, 381, 650, 788
MboI	GATC	6 cut(s)	38, 45, 256, 379, 648, 786
MboII	GAAGA	6 cut(s)	13, 212, 281, 370, 499, 752
MflI	RGATCY	2 cut(s)	38, 45
MhlI	GDGCHC	4 cut(s)	83, 148, 641, 753
MlsI	TGGCCA	1 cut(s)	346
MluCI	AATT	4 cut(s)	475, 884, 926, 942
MluNI	TGGCCA	1 cut(s)	346
MlyI	GAGTC	1 cut(s)	874
MmeI	TCCRAC	1 cut(s)	73
MnlI	CCTC	8 cut(s)	98, 214, 247, 446, 534, 820, 869, 916
Mox20I	TGGCCA	1 cut(s)	346
Mph1103I	ATGCAT	1 cut(s)	342
MscI	TGGCCA	1 cut(s)	346
Msp20I	TGGCCA	1 cut(s)	346
MspA1I	CMGCKG	1 cut(s)	530
MspI	CCGG	5 cut(s)	25, 36, 352, 635, 824
MspR9I	CCNGG	5 cut(s)	70, 84, 392, 636, 824
MvaI	CCWGG	3 cut(s)	70, 84, 392
MvnI	CGCG	1 cut(s)	219
MwoI	GCNNNNNNNGC	5 cut(s)	107, 119, 571, 722, 748
NciI	CCSGG	2 cut(s)	636, 824
NdeII	GATC	6 cut(s)	38, 45, 256, 379, 648, 786
NlaIII	CATG	5 cut(s)	211, 263, 648, 688, 896
NlaIV	GGNNCC	2 cut(s)	67, 907
NmuCI	GTSAC	1 cut(s)	656
NsiI	ATGCAT	1 cut(s)	342
PaeR7I	CTCGAG	1 cut(s)	456
PagI	TCATGA	1 cut(s)	892
PcsI	WCGNNNNNNNCGW	1 cut(s)	99
PfeI	GAWTC	3 cut(s)	443, 670, 853
PflMI	CCANNNNNTGG	1 cut(s)	246
PfoI	TCCNGGA	1 cut(s)	822
PkrI	GCNGC	5 cut(s)	109, 112, 312, 532, 564
PleI	GAGTC	1 cut(s)	874
PpsI	GAGTC	1 cut(s)	874
Ppu21I	YACGTR	1 cut(s)	900
PpuMI	RGGWCCY	1 cut(s)	66
Psp5II	RGGWCCY	1 cut(s)	66
Psp6I	CCWGG	3 cut(s)	68, 82, 390
PspGI	CCWGG	3 cut(s)	68, 82, 390
PspN4I	GGNNCC	2 cut(s)	67, 907
PspPI	GGNCC	2 cut(s)	66, 906
PspPPI	RGGWCCY	1 cut(s)	66
PspXI	VCTCGAGB	1 cut(s)	456
PstNI	CAGNNNCTG	1 cut(s)	533
PsuI	RGATCY	2 cut(s)	38, 45
PvuII	CAGCTG	1 cut(s)	530
RsaI	GTAC	2 cut(s)	253, 772
RsaNI	GTAC	2 cut(s)	252, 771
SatI	GCNGC	5 cut(s)	108, 111, 311, 531, 563
Sau3AI	GATC	6 cut(s)	38, 45, 256, 379, 648, 786
Sau96I	GGNCC	2 cut(s)	66, 906
ScaI	AGTACT	1 cut(s)	772
SchI	GAGTC	1 cut(s)	874
ScrFI	CCNGG	5 cut(s)	70, 84, 392, 636, 824
SduI	GDGCHC	4 cut(s)	83, 148, 641, 753
SexAI	ACCWGGT	1 cut(s)	390
SfaNI	GCATC	1 cut(s)	327
SfcI	CTRYAG	1 cut(s)	910
Sfr274I	CTCGAG	1 cut(s)	456
SinI	GGWCC	1 cut(s)	66
SlaI	CTCGAG	1 cut(s)	456
SmlI	CTYRAG	3 cut(s)	185, 456, 920
SmoI	CTYRAG	3 cut(s)	185, 456, 920
SnaBI	TACGTA	1 cut(s)	900
Sse9I	AATT	4 cut(s)	475, 884, 926, 942
SsiI	CCGC	3 cut(s)	5, 108, 111
SspMI	CTAG	1 cut(s)	701
StyD4I	CCNGG	5 cut(s)	68, 82, 390, 634, 822
StyI	CCWWGG	1 cut(s)	594
TaaI	ACNGT	2 cut(s)	495, 755
TaiI	ACGT	2 cut(s)	663, 902
TaqI	TCGA	3 cut(s)	77, 138, 457
TasI	AATT	4 cut(s)	475, 884, 926, 942
TatI	WGTACW	2 cut(s)	251, 770
TauI	GCSGC	2 cut(s)	110, 113
TfiI	GAWTC	3 cut(s)	443, 670, 853
TscAI	CASTG	2 cut(s)	630, 760
TseFI	GTSAC	1 cut(s)	656
TseI	GCWGC	3 cut(s)	310, 530, 562
Tsp45I	GTSAC	1 cut(s)	656
TspDTI	ATGAA	3 cut(s)	593, 792, 881
TspRI	CASTG	2 cut(s)	630, 760
Van91I	CCANNNNNTGG	1 cut(s)	246
VpaK11BI	GGWCC	1 cut(s)	66
XagI	CCTNNNNNAGG	1 cut(s)	54
XapI	RAATTY	1 cut(s)	942
XhoI	CTCGAG	1 cut(s)	456
XspI	CTAG	1 cut(s)	701
ZraI	GACGTC	1 cut(s)	661
ZrmI	AGTACT	1 cut(s)	772
Zsp2I	ATGCAT	1 cut(s)	342

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.