Rroxscaffold_6G00415210

Aldo-keto reductase family 4 member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0014906
Physical Location & Seq
Forward (+)
37452230 .. 37454615
2386 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00415210.1

Sequence Viewer

Length: 948 bp
ATGGCGAATGAAATTCAGTTCTTTGAGTTGAACACCGGAGCCAAGATCCCGTCTTTGGGATTAGGGACCTGGCAATCCAGCCCTGGTGTCGTCGGCAACGCCGTTGCCACCGCCGTCAAGGCAGGGTATCGCCACATTGATTGTGCTCAACGCTATGGAAATGAAAAGGAGATTGGTTTGGTTCTGAAGAAATTGTTTGAAGATGGTGTGGTGAAGCGCGAGGATTTGTTTATTACTTCCAAACTCTGGTGTACTGATCATGCCCCAGAAGATGTAACAGTGGCATTGGAAAAGACTTTGAGAGACTTGCAGCTGGATTATGTTGACTTGTACCTTATGCATTGGCCAGTACGCTTGAAGAAGGGCTCAGTAGATTTTAGTCCCGAAAACCTTATACAACCAGATATCCCTGCCACATGGAGAGCAATGGAATCTCTCTATGATTTGGGTAAGGCTCTAGCTATTGGTGTGAGCAATTTCTCTACAAAGAAGCTGTCAGATTTGCTGGATGTGGCACGCGTTCCTCCTGCAGTTAACCAAGTGGAGTGCCATCCTTCATGGCAGCAGACCAAGCTACATTTATTCTGTAAATCCAAGGGAGTTCATGTATCTGGATATTCACCGCTTGGTTCTCCTGGCACAACATGGATCAAAAGTGACGTCCTTGAGAATCCTGTTCTGAACACGGTTGCAGAGAAACTAGGGAAAACTGCTGCACAAGTTTCTCTTCGATGGGGTCTGCAAACAGGTCATAGTGTTCTTCCAAAGAGTACTAATGAAGCAAGGATCAAGCAGAACTTCGATGTCTTTGGCTGGTCTATCCCAGAGGACTTGTTCACCAAGCTTTCTCAAATTGAGCAGGCTAGACTGGTAAGAGGGACTTCATTTGTTCATGACACTTTTGGTCCCTACAGGAGTGTTGAGGAATTGTGGGATGGCGAAATCTAG

Protein Analysis

315

Amino Acids

35.06

Weight (kDa)

6.26

Isoelectric Point (pI)

37.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldo_ket_red PF00248 19 - 285 2.4e-51 Aldo/keto reductase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014906)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G37770 AT2G37790 AT3G53880 AT3G53880
fragaria_vesca FvH4_6g13012 FvH4_6g13012
malus_domestica MD12G1150400.v1.1
prunus_persica Prupe.6G262000_v2.0.a1
pyrus_communis pycom12g14420
rosa_chinensis RchiOBHm_Chr3g0465141
rosa_multiflora Rmu_sc0003386.1_g000011
rosa_roxburghii Rroxscaffold_6G00415210
rosa_rugosa Rorug03G0075700
rosa_samantha Rh3BG139400 Rh3DG140700
rosa_wichuraiana Rw3G011410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 663
AccB7I CCANNNNNTGG 1 cut(s) 246
AccII CGCG 2 cut(s) 219, 519
AciI CCGC 2 cut(s) 111, 623
AclWI GGATC 3 cut(s) 40, 656, 794
AcoI YGGCCR 1 cut(s) 344
AcsI RAATTY 1 cut(s) 12
AcuI CTGAAG 1 cut(s) 206
AcyI GRCGYC 1 cut(s) 660
AfaI GTAC 4 cut(s) 253, 332, 351, 772
AfiI CCNNNNNNNGG 3 cut(s) 55, 56, 246
AflIII ACRYGT 1 cut(s) 517
AgsI TTSAA 3 cut(s) 31, 200, 358
AjnI CCWGG 3 cut(s) 68, 82, 634
AjuI GAANNNNNNNTTGG 2 cut(s) 156, 188
AluBI AGCT 5 cut(s) 313, 461, 493, 574, 844
AluI AGCT 5 cut(s) 313, 461, 493, 574, 844
Alw21I GWGCWC 1 cut(s) 148
Alw26I GTCTC 1 cut(s) 297
AlwI GGATC 3 cut(s) 40, 656, 794
AoxI GGCC 1 cut(s) 344
ApeKI GCWGC 3 cut(s) 310, 562, 713
ApoI RAATTY 1 cut(s) 12
ArsI GACNNNNNNTTYG 4 cut(s) 479, 511, 645, 677
AspLEI GCGC 1 cut(s) 219
AspS9I GGNCC 2 cut(s) 66, 905
AsuHPI GGTGA 3 cut(s) 223, 612, 829
AvaII GGWCC 2 cut(s) 66, 905
BalI TGGCCA 1 cut(s) 346
BanII GRGCYC 1 cut(s) 368
Bbv12I GWGCWC 1 cut(s) 148
BbvI GCAGC 3 cut(s) 322, 574, 700
BccI CCATC 4 cut(s) 197, 558, 726, 929
BceAI ACGGC 2 cut(s) 86, 98
BciT130I CCWGG 3 cut(s) 70, 84, 636
BclI TGATCA 1 cut(s) 256
BcoDI GTCTC 1 cut(s) 297
BfaI CTAG 4 cut(s) 458, 701, 864, 946
BfmI CTRYAG 2 cut(s) 528, 910
BglI GCCNNNNNGGC 1 cut(s) 119
BisI GCNGC 3 cut(s) 311, 563, 714
BlsI GCNGC 3 cut(s) 312, 564, 715
BmcAI AGTACT 1 cut(s) 772
Bme1390I CCNGG 3 cut(s) 70, 84, 636
Bme18I GGWCC 2 cut(s) 66, 905
BmgT120I GGNCC 2 cut(s) 66, 905
BmiI GGNNCC 3 cut(s) 40, 67, 907
BmrFI CCNGG 3 cut(s) 70, 84, 636
BpuEI CTTGAG 1 cut(s) 686
BsaHI GRCGYC 1 cut(s) 660
BsaJI CCNNGG 2 cut(s) 82, 594
BsaWI WCCGGW 1 cut(s) 35
BsaXI ACNNNNNCTCC 2 cut(s) 591, 621
Bsc4I CCNNNNNNNGG 3 cut(s) 55, 56, 246
Bse1I ACTGG 2 cut(s) 347, 873
Bse3DI GCAATG 1 cut(s) 432
BseBI CCWGG 3 cut(s) 70, 84, 636
BseDI CCNNGG 2 cut(s) 82, 594
BseGI GGATG 3 cut(s) 514, 550, 940
BseLI CCNNNNNNNGG 3 cut(s) 55, 56, 246
BseMI GCAATG 1 cut(s) 432
BseMII CTCAG 1 cut(s) 381
BseNI ACTGG 2 cut(s) 347, 873
BseXI GCAGC 3 cut(s) 322, 574, 700
BsgI GTGCAG 1 cut(s) 699
Bsh1236I CGCG 2 cut(s) 219, 519
BshFI GGCC 1 cut(s) 346
BsiHKAI GWGCWC 1 cut(s) 148
BsiSI CCGG 1 cut(s) 36
BslFI GGGAC 4 cut(s) 79, 366, 891, 892
BslI CCNNNNNNNGG 3 cut(s) 55, 56, 246
BsmAI GTCTC 1 cut(s) 297
BsmFI GGGAC 4 cut(s) 79, 366, 891, 892
BsnI GGCC 1 cut(s) 346
Bsp1286I GDGCHC 2 cut(s) 148, 368
Bsp143I GATC 4 cut(s) 45, 256, 648, 786
BspACI CCGC 2 cut(s) 111, 623
BspANI GGCC 1 cut(s) 346
BspCNI CTCAG 1 cut(s) 380
BspFNI CGCG 2 cut(s) 219, 519
BspHI TCATGA 1 cut(s) 892
BspLI GGNNCC 3 cut(s) 40, 67, 907
BspMAI CTGCAG 1 cut(s) 532
BspPI GGATC 3 cut(s) 40, 656, 794
BsrDI GCAATG 1 cut(s) 432
BsrI ACTGG 2 cut(s) 347, 873
BssECI CCNNGG 2 cut(s) 82, 594
BssMI GATC 4 cut(s) 45, 256, 648, 786
BssNI GRCGYC 1 cut(s) 660
BssT1I CCWWGG 1 cut(s) 594
Bst2UI CCWGG 3 cut(s) 70, 84, 636
Bst4CI ACNGT 2 cut(s) 280, 688
Bst6I CTCTTC 1 cut(s) 732
BstACI GRCGYC 1 cut(s) 660
BstC8I GCNNGC 2 cut(s) 517, 861
BstDEI CTNAG 1 cut(s) 367
BstF5I GGATG 3 cut(s) 514, 550, 940
BstFNI CGCG 2 cut(s) 219, 519
BstHHI GCGC 1 cut(s) 219
BstKTI GATC 4 cut(s) 48, 259, 651, 789
BstMAI GTCTC 1 cut(s) 297
BstMBI GATC 4 cut(s) 45, 256, 648, 786
BstMWI GCNNNNNNNGC 2 cut(s) 119, 571
BstNI CCWGG 3 cut(s) 70, 84, 636
BstSCI CCNGG 3 cut(s) 68, 82, 634
BstSFI CTRYAG 2 cut(s) 528, 910
BstUI CGCG 2 cut(s) 219, 519
BstV1I GCAGC 3 cut(s) 322, 574, 700
BstX2I RGATCY 1 cut(s) 45
BstYI RGATCY 1 cut(s) 45
BsuRI GGCC 1 cut(s) 346
BtsCI GGATG 3 cut(s) 514, 550, 940
BtsIMutI CAGTG 1 cut(s) 285
Cac8I GCNNGC 2 cut(s) 517, 861
CciI TCATGA 1 cut(s) 892
CfoI GCGC 1 cut(s) 219
Cfr13I GGNCC 2 cut(s) 66, 905
Csp6I GTAC 4 cut(s) 252, 331, 350, 771
CspCI CAANNNNNGTGG 2 cut(s) 122, 157
CviAII CATG 6 cut(s) 260, 417, 558, 605, 645, 893
CviQI GTAC 4 cut(s) 252, 331, 350, 771
DdeI CTNAG 1 cut(s) 367
DpnI GATC 4 cut(s) 47, 258, 650, 788
DpnII GATC 4 cut(s) 45, 256, 648, 786
EaeI YGGCCR 1 cut(s) 344
Eam1104I CTCTTC 1 cut(s) 732
EarI CTCTTC 1 cut(s) 732
Eco130I CCWWGG 1 cut(s) 594
Eco24I GRGCYC 1 cut(s) 368
Eco32I GATATC 1 cut(s) 406
Eco47I GGWCC 2 cut(s) 66, 905
Eco57I CTGAAG 1 cut(s) 206
EcoO109I RGGNCCY 1 cut(s) 66
EcoRII CCWGG 3 cut(s) 68, 82, 634
EcoRV GATATC 1 cut(s) 406
EcoT14I CCWWGG 1 cut(s) 594
EcoT22I ATGCAT 1 cut(s) 342
EcoT38I GRGCYC 1 cut(s) 368
ErhI CCWWGG 1 cut(s) 594
FaeI CATG 6 cut(s) 263, 420, 561, 608, 648, 896
FalI AAGNNNNNCTT 6 cut(s) 711, 743, 782, 814, 865, 897
FaqI GGGAC 4 cut(s) 79, 366, 891, 892
FatI CATG 6 cut(s) 259, 416, 557, 604, 644, 892
FbaI TGATCA 1 cut(s) 256
Fnu4HI GCNGC 3 cut(s) 311, 563, 714
FokI GGATG 2 cut(s) 521, 537
FriOI GRGCYC 1 cut(s) 368
Fsp4HI GCNGC 3 cut(s) 311, 563, 714
FspBI CTAG 4 cut(s) 458, 701, 864, 946
GlaI GCGC 1 cut(s) 218
GluI GCNGC 3 cut(s) 311, 563, 714
HaeIII GGCC 1 cut(s) 346
HapII CCGG 1 cut(s) 36
HhaI GCGC 1 cut(s) 219
Hin1I GRCGYC 1 cut(s) 660
Hin1II CATG 6 cut(s) 263, 420, 561, 608, 648, 896
Hin6I GCGC 1 cut(s) 217
HinP1I GCGC 1 cut(s) 217
HincII GTYRAC 2 cut(s) 325, 535
HindII GTYRAC 2 cut(s) 325, 535
HindIII AAGCTT 1 cut(s) 842
HinfI GANTC 2 cut(s) 431, 670
HpaI GTTAAC 1 cut(s) 535
HpaII CCGG 1 cut(s) 36
HphI GGTGA 3 cut(s) 223, 612, 829
Hpy166II GTNNAC 4 cut(s) 252, 325, 535, 837
Hpy188I TCNGA 3 cut(s) 186, 499, 681
Hpy188III TCNNGA 3 cut(s) 383, 612, 893
Hpy8I GTNNAC 4 cut(s) 252, 325, 535, 837
Hpy99I CGWCG 1 cut(s) 95
HpyAV CCTTC 2 cut(s) 355, 564
HpyCH4III ACNGT 2 cut(s) 280, 688
HpyCH4IV ACGT 1 cut(s) 660
HpyCH4V TGCA 6 cut(s) 310, 340, 530, 692, 716, 742
HpyF10VI GCNNNNNNNGC 2 cut(s) 119, 571
HpyF3I CTNAG 1 cut(s) 367
HpySE526I ACGT 1 cut(s) 660
Hsp92I GRCGYC 1 cut(s) 660
Hsp92II CATG 6 cut(s) 263, 420, 561, 608, 648, 896
HspAI GCGC 1 cut(s) 217
Ksp22I TGATCA 1 cut(s) 256
KspAI GTTAAC 1 cut(s) 535
Kzo9I GATC 4 cut(s) 45, 256, 648, 786
LmnI GCTCC 1 cut(s) 38
Lsp1109I GCAGC 3 cut(s) 322, 574, 700
MaeI CTAG 4 cut(s) 458, 701, 864, 946
MaeII ACGT 1 cut(s) 660
MaeIII GTNAC 2 cut(s) 274, 656
MalI GATC 4 cut(s) 47, 258, 650, 788
MboI GATC 4 cut(s) 45, 256, 648, 786
MboII GAAGA 6 cut(s) 199, 212, 281, 370, 719, 752
MflI RGATCY 1 cut(s) 45
MhlI GDGCHC 2 cut(s) 148, 368
MlsI TGGCCA 1 cut(s) 346
MluCI AATT 5 cut(s) 12, 191, 475, 852, 926
MluI ACGCGT 1 cut(s) 517
MluNI TGGCCA 1 cut(s) 346
MnlI CCTC 5 cut(s) 214, 534, 820, 869, 916
Mox20I TGGCCA 1 cut(s) 346
Mph1103I ATGCAT 1 cut(s) 342
MscI TGGCCA 1 cut(s) 346
MseI TTAA 1 cut(s) 534
Msp20I TGGCCA 1 cut(s) 346
MspA1I CMGCKG 1 cut(s) 313
MspI CCGG 1 cut(s) 36
MspR9I CCNGG 3 cut(s) 70, 84, 636
MvaI CCWGG 3 cut(s) 70, 84, 636
MvnI CGCG 2 cut(s) 219, 519
MwoI GCNNNNNNNGC 2 cut(s) 119, 571
NdeII GATC 4 cut(s) 45, 256, 648, 786
NlaIII CATG 6 cut(s) 263, 420, 561, 608, 648, 896
NlaIV GGNNCC 3 cut(s) 40, 67, 907
NmuCI GTSAC 1 cut(s) 656
NsiI ATGCAT 1 cut(s) 342
PagI TCATGA 1 cut(s) 892
PcsI WCGNNNNNNNCGW 1 cut(s) 99
PfeI GAWTC 2 cut(s) 431, 670
PflMI CCANNNNNTGG 1 cut(s) 246
PkrI GCNGC 3 cut(s) 312, 564, 715
PpuMI RGGWCCY 1 cut(s) 66
Psp5II RGGWCCY 1 cut(s) 66
Psp6I CCWGG 3 cut(s) 68, 82, 634
PspGI CCWGG 3 cut(s) 68, 82, 634
PspN4I GGNNCC 3 cut(s) 40, 67, 907
PspPI GGNCC 2 cut(s) 66, 905
PspPPI RGGWCCY 1 cut(s) 66
PstI CTGCAG 1 cut(s) 532
PsuI RGATCY 1 cut(s) 45
PvuII CAGCTG 1 cut(s) 313
RsaI GTAC 4 cut(s) 253, 332, 351, 772
RsaNI GTAC 4 cut(s) 252, 331, 350, 771
SaqAI TTAA 1 cut(s) 534
SatI GCNGC 3 cut(s) 311, 563, 714
Sau3AI GATC 4 cut(s) 45, 256, 648, 786
Sau96I GGNCC 2 cut(s) 66, 905
ScaI AGTACT 1 cut(s) 772
ScrFI CCNGG 3 cut(s) 70, 84, 636
SduI GDGCHC 2 cut(s) 148, 368
SfcI CTRYAG 2 cut(s) 528, 910
SinI GGWCC 2 cut(s) 66, 905
SmlI CTYRAG 1 cut(s) 665
SmoI CTYRAG 1 cut(s) 665
Sse9I AATT 5 cut(s) 12, 191, 475, 852, 926
SsiI CCGC 2 cut(s) 111, 623
SspMI CTAG 4 cut(s) 458, 701, 864, 946
StyD4I CCNGG 3 cut(s) 68, 82, 634
StyI CCWWGG 1 cut(s) 594
TaaI ACNGT 2 cut(s) 280, 688
TaiI ACGT 1 cut(s) 663
TaqI TCGA 2 cut(s) 730, 801
TasI AATT 5 cut(s) 12, 191, 475, 852, 926
TatI WGTACW 2 cut(s) 251, 770
TfiI GAWTC 2 cut(s) 431, 670
Tru1I TTAA 1 cut(s) 534
Tru9I TTAA 1 cut(s) 534
TscAI CASTG 1 cut(s) 285
TseFI GTSAC 1 cut(s) 656
TseI GCWGC 3 cut(s) 310, 562, 713
Tsp45I GTSAC 1 cut(s) 656
TspDTI ATGAA 7 cut(s) 24, 177, 546, 593, 792, 873, 881
TspRI CASTG 1 cut(s) 285
Van91I CCANNNNNTGG 1 cut(s) 246
VpaK11BI GGWCC 2 cut(s) 66, 905
XapI RAATTY 1 cut(s) 12
XspI CTAG 4 cut(s) 458, 701, 864, 946
ZraI GACGTC 1 cut(s) 661
ZrmI AGTACT 1 cut(s) 772
Zsp2I ATGCAT 1 cut(s) 342
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.