FvH4_6g14030

RING-H2 finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0012772
Physical Location & Seq
Reverse (-)
8598911 .. 8599429
519 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g14030.t1

Sequence Viewer

Length: 519 bp
ATGGAGAGCTCACCGGCCATCCTAAACAAACCACCCCCGCCCTTCCCTGCGCCACCGCGCAGCGTCGACTTCTCCGCCCTCGAGTTCATCCTCGGCCTTCTCGCCGTCATCACCGTCCCCGCCTTAATATACGCTGTCTTCTTCGCCATCAAGTGCCCTCCCAGCCTCTTCCGCTATCGACCCCGCCGGAGCTCCACCACCCCCCACGGCGACCCTTCACAAGCTCTTCCGGAGGTGATGTCCGACCTCAAGTACCAGAAGGACTCACACGTCAAGGAAATCGGGACCGAGTGCCCGGTTTGCTTATCGGCCTTTGACGACGGCGAAGAGGTGAGGCAGCTCAGTGTGTGTAAGCACTCGTTTCATAGTACGTGCATCAACATGTGGCTCAAGGACCACTCTAATTGCCCGATTTGCCGCGCGAACATCGCCGTCGATCGGAGTAGAACGGCGGCGTCCGGTAGAGAACATCGGGATGATCTGCAACAAGGTTTGCCGGATGCTTCCGCCATGGTTTGA

Protein Analysis

173

Amino Acids

18.98

Weight (kDa)

6.58

Isoelectric Point (pI)

68.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 94 - 140 7.1e-09 RING-H2 zinc finger domain
zf-RING_2 PF13639 97 - 140 1.1e-13 Ring finger domain
zf-RING_11 PF17123 97 - 126 6.8e-09 RING-like zinc finger
zf-C3HC4_2 PF13923 97 - 139 3.3e-09 Zinc finger, C3HC4 type (RING finger)
zf-ANAPC11 PF12861 97 - 145 6.4e-07 Anaphase-promoting complex subunit 11 RING-H2 finger
zf-C3HC4 PF00097 98 - 139 1.9e-07 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 98 - 137 1.2e-06 RING-type zinc-finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012772)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G37580
fragaria_vesca FvH4_6g14030
malus_domestica MD12G1078700.v1.1
prunus_persica Prupe.6G253000_v2.0.a1
pyrus_communis pycom04g11330 pycom12g13480
rosa_chinensis RchiOBHm_Chr3g0466911
rosa_laevigata RLG00000020757 RLG00000024546
rosa_multiflora Rmu_co8052150.1_g000001 Rmu_sc0001193.1_g000017
rosa_roxburghii Rroxscaffold_6G00414070
rosa_rugosa Rorug03G0084200
rosa_samantha Rh3AG132100 Rh3BG153200 Rh3CG153200 Rh3DG154100
rosa_wichuraiana Rw3G012470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 269
AccI GTMKAC 1 cut(s) 66
AccII CGCG 3 cut(s) 58, 420, 422
AccIII TCCGGA 1 cut(s) 229
AciI CCGC 9 cut(s) 38, 56, 75, 120, 172, 184, 418, 452, 507
AcoI YGGCCR 1 cut(s) 15
AcyI GRCGYC 1 cut(s) 455
AfaI GTAC 2 cut(s) 254, 370
AfiI CCNNNNNNNGG 1 cut(s) 438
AflIII ACRYGT 2 cut(s) 268, 381
AjiI CACGTC 1 cut(s) 271
AluBI AGCT 4 cut(s) 9, 192, 224, 340
AluI AGCT 4 cut(s) 9, 192, 224, 340
Alw21I GWGCWC 2 cut(s) 11, 194
Ama87I CYCGRG 1 cut(s) 80
Aor13HI TCCGGA 1 cut(s) 229
AoxI GGCC 3 cut(s) 15, 94, 309
ApeKI GCWGC 2 cut(s) 60, 337
AspLEI GCGC 3 cut(s) 52, 60, 422
AspS9I GGNCC 2 cut(s) 285, 394
AsuC2I CCSGG 1 cut(s) 296
AsuHPI GGTGA 4 cut(s) 3, 103, 247, 343
AvaI CYCGRG 1 cut(s) 80
AvaII GGWCC 2 cut(s) 285, 394
BaeGI GKGCMC 2 cut(s) 158, 296
BanII GRGCYC 2 cut(s) 11, 194
BbsI GAAGAC 1 cut(s) 130
Bbv12I GWGCWC 2 cut(s) 11, 194
BbvI GCAGC 2 cut(s) 72, 349
BccI CCATC 2 cut(s) 26, 155
BceAI ACGGC 5 cut(s) 89, 223, 337, 416, 465
BcnI CCSGG 1 cut(s) 296
BisI GCNGC 4 cut(s) 61, 338, 418, 453
BlsI GCNGC 4 cut(s) 62, 339, 419, 454
Bme1390I CCNGG 1 cut(s) 296
Bme18I GGWCC 2 cut(s) 285, 394
BmeT110I CYCGRG 1 cut(s) 80
BmgBI CACGTC 1 cut(s) 271
BmgT120I GGNCC 2 cut(s) 285, 394
BmiI GGNNCC 1 cut(s) 286
BmrFI CCNGG 1 cut(s) 296
BmsI GCATC 2 cut(s) 384, 490
BpiI GAAGAC 1 cut(s) 130
BpuEI CTTGAG 2 cut(s) 233, 374
BpuMI CCSGG 1 cut(s) 296
BsaAI YACGTR 1 cut(s) 372
BsaHI GRCGYC 1 cut(s) 455
BsaJI CCNNGG 3 cut(s) 91, 205, 510
BsaWI WCCGGW 2 cut(s) 229, 458
BsaXI ACNNNNNCTCC 2 cut(s) 224, 254
Bsc4I CCNNNNNNNGG 1 cut(s) 438
Bse118I RCCGGY 1 cut(s) 13
BseAI TCCGGA 1 cut(s) 229
BseDI CCNNGG 3 cut(s) 91, 205, 510
BseGI GGATG 4 cut(s) 18, 87, 481, 505
BseLI CCNNNNNNNGG 1 cut(s) 438
BseMII CTCAG 1 cut(s) 355
BseSI GKGCMC 2 cut(s) 158, 296
BseXI GCAGC 2 cut(s) 72, 349
BseYI CCCAGC 1 cut(s) 161
Bsh1236I CGCG 3 cut(s) 58, 420, 422
Bsh1285I CGRYCG 1 cut(s) 439
BshFI GGCC 3 cut(s) 17, 96, 311
BsiEI CGRYCG 1 cut(s) 439
BsiHKAI GWGCWC 2 cut(s) 11, 194
BsiHKCI CYCGRG 1 cut(s) 80
BsiSI CCGG 6 cut(s) 14, 187, 230, 296, 459, 497
BslFI GGGAC 2 cut(s) 101, 298
BslI CCNNNNNNNGG 1 cut(s) 438
BsmFI GGGAC 2 cut(s) 101, 298
BsnI GGCC 3 cut(s) 17, 96, 311
BsoBI CYCGRG 1 cut(s) 80
Bsp1286I GDGCHC 4 cut(s) 11, 158, 194, 296
Bsp13I TCCGGA 1 cut(s) 229
Bsp143I GATC 2 cut(s) 436, 478
Bsp19I CCATGG 1 cut(s) 510
BspACI CCGC 9 cut(s) 38, 56, 75, 120, 172, 184, 418, 452, 507
BspANI GGCC 3 cut(s) 17, 96, 311
BspCNI CTCAG 1 cut(s) 354
BspEI TCCGGA 1 cut(s) 229
BspFNI CGCG 3 cut(s) 58, 420, 422
BspLI GGNNCC 1 cut(s) 286
BspQI GCTCTTC 1 cut(s) 231
BsrFI RCCGGY 1 cut(s) 13
BssAI RCCGGY 1 cut(s) 13
BssECI CCNNGG 3 cut(s) 91, 205, 510
BssMI GATC 2 cut(s) 436, 478
BssNI GRCGYC 1 cut(s) 455
BssT1I CCWWGG 1 cut(s) 510
Bst4CI ACNGT 1 cut(s) 115
Bst6I CTCTTC 3 cut(s) 173, 231, 321
BstACI GRCGYC 1 cut(s) 455
BstBAI YACGTR 1 cut(s) 372
BstDEI CTNAG 1 cut(s) 341
BstDSI CCRYGG 2 cut(s) 205, 510
BstF5I GGATG 4 cut(s) 18, 87, 481, 505
BstFNI CGCG 3 cut(s) 58, 420, 422
BstHHI GCGC 3 cut(s) 52, 60, 422
BstKTI GATC 2 cut(s) 439, 481
BstMBI GATC 2 cut(s) 436, 478
BstMCI CGRYCG 1 cut(s) 439
BstMWI GCNNNNNNNGC 5 cut(s) 162, 171, 300, 414, 428
BstNSI RCATGY 1 cut(s) 385
BstSCI CCNGG 1 cut(s) 294
BstSLI GKGCMC 2 cut(s) 158, 296
BstUI CGCG 3 cut(s) 58, 420, 422
BstV1I GCAGC 2 cut(s) 72, 349
BstV2I GAAGAC 1 cut(s) 130
BsuRI GGCC 3 cut(s) 17, 96, 311
BtgI CCRYGG 2 cut(s) 205, 510
BtgZI GCGATG 1 cut(s) 412
BtrI CACGTC 1 cut(s) 271
BtsCI GGATG 4 cut(s) 18, 87, 481, 505
BtsIMutI CAGTG 1 cut(s) 349
CfoI GCGC 3 cut(s) 52, 60, 422
Cfr10I RCCGGY 1 cut(s) 13
Cfr13I GGNCC 2 cut(s) 285, 394
CseI GACGC 2 cut(s) 52, 444
Csp6I GTAC 2 cut(s) 253, 369
CspCI CAANNNNNGTGG 2 cut(s) 386, 421
CviAII CATG 2 cut(s) 382, 511
CviJI RGCY 9 cut(s) 9, 17, 96, 165, 192, 224, 311, 340, 388
CviKI_1 RGCY 9 cut(s) 9, 17, 96, 165, 192, 224, 311, 340, 388
CviQI GTAC 2 cut(s) 253, 369
DdeI CTNAG 1 cut(s) 341
DpnI GATC 2 cut(s) 438, 480
DpnII GATC 2 cut(s) 436, 478
DrdI GACNNNNNNGTC 1 cut(s) 269
DseDI GACNNNNNNGTC 1 cut(s) 269
EaeI YGGCCR 1 cut(s) 15
Eam1104I CTCTTC 3 cut(s) 173, 231, 321
EarI CTCTTC 3 cut(s) 173, 231, 321
EciI GGCGGA 2 cut(s) 64, 496
Ecl136II GAGCTC 2 cut(s) 9, 192
Eco130I CCWWGG 1 cut(s) 510
Eco24I GRGCYC 2 cut(s) 11, 194
Eco47I GGWCC 2 cut(s) 285, 394
Eco53kI GAGCTC 2 cut(s) 9, 192
Eco88I CYCGRG 1 cut(s) 80
EcoICRI GAGCTC 2 cut(s) 9, 192
EcoT14I CCWWGG 1 cut(s) 510
EcoT38I GRGCYC 2 cut(s) 11, 194
ErhI CCWWGG 1 cut(s) 510
FaeI CATG 2 cut(s) 385, 514
FaiI YATR 4 cut(s) 130, 366, 383, 512
FaqI GGGAC 2 cut(s) 101, 298
FatI CATG 2 cut(s) 381, 510
FauI CCCGC 3 cut(s) 45, 127, 191
FblI GTMKAC 1 cut(s) 66
Fnu4HI GCNGC 4 cut(s) 61, 338, 418, 453
FokI GGATG 4 cut(s) 5, 74, 488, 512
FriOI GRGCYC 2 cut(s) 11, 194
Fsp4HI GCNGC 4 cut(s) 61, 338, 418, 453
GlaI GCGC 3 cut(s) 51, 59, 421
GluI GCNGC 4 cut(s) 61, 338, 418, 453
GsaI CCCAGC 1 cut(s) 165
HaeIII GGCC 3 cut(s) 17, 96, 311
HapII CCGG 6 cut(s) 14, 187, 230, 296, 459, 497
HgaI GACGC 2 cut(s) 52, 444
HhaI GCGC 3 cut(s) 52, 60, 422
Hin1I GRCGYC 1 cut(s) 455
Hin1II CATG 2 cut(s) 385, 514
Hin6I GCGC 3 cut(s) 50, 58, 420
HinP1I GCGC 3 cut(s) 50, 58, 420
HincII GTYRAC 1 cut(s) 67
HindII GTYRAC 1 cut(s) 67
HinfI GANTC 1 cut(s) 263
HpaII CCGG 6 cut(s) 14, 187, 230, 296, 459, 497
HphI GGTGA 4 cut(s) 3, 103, 247, 343
Hpy166II GTNNAC 1 cut(s) 67
Hpy188I TCNGA 2 cut(s) 244, 441
Hpy188III TCNNGA 3 cut(s) 230, 283, 473
Hpy8I GTNNAC 1 cut(s) 67
Hpy99I CGWCG 3 cut(s) 68, 323, 437
HpyAV CCTTC 4 cut(s) 52, 107, 225, 253
HpyCH4III ACNGT 1 cut(s) 115
HpyCH4IV ACGT 2 cut(s) 270, 371
HpyCH4V TGCA 2 cut(s) 375, 484
HpyF10VI GCNNNNNNNGC 5 cut(s) 162, 171, 300, 414, 428
HpyF3I CTNAG 1 cut(s) 341
HpySE526I ACGT 2 cut(s) 270, 371
Hsp92I GRCGYC 1 cut(s) 455
Hsp92II CATG 2 cut(s) 385, 514
HspAI GCGC 3 cut(s) 50, 58, 420
Kpn2I TCCGGA 1 cut(s) 229
Kzo9I GATC 2 cut(s) 436, 478
LguI GCTCTTC 1 cut(s) 231
LmnI GCTCC 2 cut(s) 189, 197
LpnPI CCDG 9 cut(s) 27, 60, 175, 200, 243, 269, 309, 472, 510
Lsp1109I GCAGC 2 cut(s) 72, 349
LweI GCATC 2 cut(s) 384, 490
MaeII ACGT 2 cut(s) 270, 371
MalI GATC 2 cut(s) 438, 480
MboI GATC 2 cut(s) 436, 478
MboII GAAGA 5 cut(s) 130, 133, 160, 218, 338
MhlI GDGCHC 4 cut(s) 11, 158, 194, 296
MluCI AATT 1 cut(s) 403
MlyI GAGTC 1 cut(s) 257
MmeI TCCRAC 1 cut(s) 267
MnlI CCTC 8 cut(s) 89, 101, 168, 176, 226, 257, 322, 327
MroI TCCGGA 1 cut(s) 229
MseI TTAA 1 cut(s) 125
MslI CAYNNNNRTG 2 cut(s) 380, 474
MspI CCGG 6 cut(s) 14, 187, 230, 296, 459, 497
MspR9I CCNGG 1 cut(s) 296
MvnI CGCG 3 cut(s) 58, 420, 422
MwoI GCNNNNNNNGC 5 cut(s) 162, 171, 300, 414, 428
NciI CCSGG 1 cut(s) 296
NcoI CCATGG 1 cut(s) 510
NdeII GATC 2 cut(s) 436, 478
NlaIII CATG 2 cut(s) 385, 514
NlaIV GGNNCC 1 cut(s) 286
NmeAIII GCCGAG 1 cut(s) 72
NspI RCATGY 1 cut(s) 385
PaeR7I CTCGAG 1 cut(s) 80
PciI ACATGT 1 cut(s) 381
PciSI GCTCTTC 1 cut(s) 231
PkrI GCNGC 4 cut(s) 62, 339, 419, 454
Ple19I CGATCG 1 cut(s) 439
PleI GAGTC 1 cut(s) 257
PpsI GAGTC 1 cut(s) 257
Ppu21I YACGTR 1 cut(s) 372
PscI ACATGT 1 cut(s) 381
Psp124BI GAGCTC 2 cut(s) 11, 194
PspFI CCCAGC 1 cut(s) 161
PspN4I GGNNCC 1 cut(s) 286
PspPI GGNCC 2 cut(s) 285, 394
PspXI VCTCGAGB 1 cut(s) 80
PvuI CGATCG 1 cut(s) 439
RsaI GTAC 2 cut(s) 254, 370
RsaNI GTAC 2 cut(s) 253, 369
RseI CAYNNNNRTG 2 cut(s) 380, 474
SacI GAGCTC 2 cut(s) 11, 194
SalI GTCGAC 1 cut(s) 65
SapI GCTCTTC 1 cut(s) 231
SaqAI TTAA 1 cut(s) 125
SatI GCNGC 4 cut(s) 61, 338, 418, 453
Sau3AI GATC 2 cut(s) 436, 478
Sau96I GGNCC 2 cut(s) 285, 394
SchI GAGTC 1 cut(s) 257
ScrFI CCNGG 1 cut(s) 296
SduI GDGCHC 4 cut(s) 11, 158, 194, 296
SfaNI GCATC 2 cut(s) 384, 490
Sfr274I CTCGAG 1 cut(s) 80
SinI GGWCC 2 cut(s) 285, 394
SlaI CTCGAG 1 cut(s) 80
SmiMI CAYNNNNRTG 2 cut(s) 380, 474
SmlI CTYRAG 3 cut(s) 80, 248, 389
SmoI CTYRAG 3 cut(s) 80, 248, 389
Sse9I AATT 1 cut(s) 403
SsiI CCGC 9 cut(s) 38, 56, 75, 120, 172, 184, 418, 452, 507
SstI GAGCTC 2 cut(s) 11, 194
StyD4I CCNGG 1 cut(s) 294
StyI CCWWGG 1 cut(s) 510
TaaI ACNGT 1 cut(s) 115
TaiI ACGT 2 cut(s) 273, 374
TaqI TCGA 4 cut(s) 66, 81, 178, 435
TaqII GACCGA 1 cut(s) 302
TasI AATT 1 cut(s) 403
TauI GCSGC 2 cut(s) 420, 455
Tru1I TTAA 1 cut(s) 125
Tru9I TTAA 1 cut(s) 125
TscAI CASTG 1 cut(s) 349
TseI GCWGC 2 cut(s) 60, 337
TspDTI ATGAA 2 cut(s) 76, 353
TspRI CASTG 1 cut(s) 349
VpaK11BI GGWCC 2 cut(s) 285, 394
XceI RCATGY 1 cut(s) 385
XhoI CTCGAG 1 cut(s) 80
XmiI GTMKAC 1 cut(s) 66
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.