MD12G1078700.v1.1

RING-H2 finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0012772
Physical Location & Seq
Reverse (-)
9597412 .. 9597963
552 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1078700.v1.1.491

Sequence Viewer

Length: 552 bp
ATGGACAGCCCACCTACCGTTACCGTTCTGAACAAACCACCGCCACCTTTCCCAGCACCGCCGCGAAGCGTCGACTTCTCTGCCCTCGAATTCATCCTTGGCCTCATCGCCGTCATCACCATCCCCGCTCTCGTCTACGCCTTCTTCTTCGCCATCAAGTGCCCTTCCAACCCTTTCCGCCGGCGACCCCGCCGGACCTCCGCCACCGTCCCCAGCCCCACCCAACACGAACCCAACACCGAAAACAAAAACGACGTCATTTCGGAGCTCAAGTACCAGAAGGAAACTCACTTTAAGGAAATCGGAACTGAGTGCCCTGTTTGCTTGTCTCTGTTTGCTGACGGCGAAGAGGTCCGGCAGCTCTGCGTCTGCAAACACTCTTTCCACGCTCTGTGCATAAACATGTGGCTTAAGGAACACTCCAACTGCCCCATTTGTCGAGCTTCGGTCGCCGTTGATCGGAGTAGTAGTAATAATCGGACGGCGGGTGCTGCAGCTAGAGGTAGTGATGATCTGCAACAAGGTTTGCGTGATGCTTCCGCCATGGTTTGA

Protein Analysis

184

Amino Acids

20.17

Weight (kDa)

8.14

Isoelectric Point (pI)

61.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 104 - 147 2.2e-11 Ring finger domain
zf-C3HC4_2 PF13923 104 - 146 1.1e-07 Zinc finger, C3HC4 type (RING finger)
zf-RING_11 PF17123 104 - 133 6.5e-06 RING-like zinc finger
zf-rbx1 PF12678 105 - 147 7.3e-07 RING-H2 zinc finger domain
zf-C3HC4 PF00097 105 - 146 8.9e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012772)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G37580
fragaria_vesca FvH4_6g14030
malus_domestica MD12G1078700.v1.1
prunus_persica Prupe.6G253000_v2.0.a1
pyrus_communis pycom04g11330 pycom12g13480
rosa_chinensis RchiOBHm_Chr3g0466911
rosa_laevigata RLG00000020757 RLG00000024546
rosa_multiflora Rmu_co8052150.1_g000001 Rmu_sc0001193.1_g000017
rosa_roxburghii Rroxscaffold_6G00414070
rosa_rugosa Rorug03G0084200
rosa_samantha Rh3AG132100 Rh3BG153200 Rh3CG153200 Rh3DG154100
rosa_wichuraiana Rw3G012470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 258
AccBSI CCGCTC 1 cut(s) 128
AccI GTMKAC 2 cut(s) 72, 135
AccII CGCG 1 cut(s) 64
AciI CCGC 9 cut(s) 41, 59, 62, 126, 178, 190, 201, 485, 540
AcsI RAATTY 1 cut(s) 89
AcyI GRCGYC 1 cut(s) 255
AfaI GTAC 1 cut(s) 275
AfiI CCNNNNNNNGG 1 cut(s) 459
AflII CTTAAG 1 cut(s) 410
AflIII ACRYGT 1 cut(s) 402
AjuI GAANNNNNNNTTGG 2 cut(s) 81, 113
AluBI AGCT 4 cut(s) 268, 361, 443, 497
AluI AGCT 4 cut(s) 268, 361, 443, 497
Alw21I GWGCWC 1 cut(s) 270
Alw26I GTCTC 1 cut(s) 333
AoxI GGCC 1 cut(s) 100
ApeKI GCWGC 3 cut(s) 358, 491, 494
ApoI RAATTY 1 cut(s) 89
ArsI GACNNNNNNTTYG 2 cut(s) 240, 272
AspS9I GGNCC 2 cut(s) 195, 352
AsuHPI GGTGA 1 cut(s) 109
AvaII GGWCC 2 cut(s) 195, 352
BaeGI GKGCMC 2 cut(s) 164, 317
BanII GRGCYC 1 cut(s) 270
Bbv12I GWGCWC 1 cut(s) 270
BbvI GCAGC 3 cut(s) 370, 478, 506
BccI CCATC 2 cut(s) 128, 161
BceAI ACGGC 4 cut(s) 95, 358, 437, 498
BcgI CGANNNNNNTGC 2 cut(s) 62, 96
BcoDI GTCTC 1 cut(s) 333
BfaI CTAG 1 cut(s) 498
BfmI CTRYAG 1 cut(s) 492
BfrI CTTAAG 1 cut(s) 410
BisI GCNGC 4 cut(s) 62, 359, 492, 495
BlsI GCNGC 4 cut(s) 63, 360, 493, 496
Bme18I GGWCC 2 cut(s) 195, 352
BmgT120I GGNCC 2 cut(s) 195, 352
BmsI GCATC 1 cut(s) 523
BpuEI CTTGAG 1 cut(s) 254
BsaHI GRCGYC 1 cut(s) 255
BsaJI CCNNGG 2 cut(s) 97, 543
BsaXI ACNNNNNCTCC 4 cut(s) 257, 287, 454, 484
Bsc4I CCNNNNNNNGG 1 cut(s) 459
Bse118I RCCGGY 1 cut(s) 180
BseDI CCNNGG 2 cut(s) 97, 543
BseGI GGATG 2 cut(s) 93, 120
BseLI CCNNNNNNNGG 1 cut(s) 459
BseMII CTCAG 1 cut(s) 300
BseSI GKGCMC 2 cut(s) 164, 317
BseXI GCAGC 3 cut(s) 370, 478, 506
BseYI CCCAGC 2 cut(s) 52, 212
Bsh1236I CGCG 1 cut(s) 64
Bsh1285I CGRYCG 1 cut(s) 450
BshFI GGCC 1 cut(s) 102
BsiEI CGRYCG 1 cut(s) 450
BsiHKAI GWGCWC 1 cut(s) 270
BsiSI CCGG 3 cut(s) 181, 193, 355
BslFI GGGAC 1 cut(s) 194
BslI CCNNNNNNNGG 1 cut(s) 459
BsmAI GTCTC 1 cut(s) 333
BsmFI GGGAC 1 cut(s) 194
BsnI GGCC 1 cut(s) 102
Bsp1286I GDGCHC 3 cut(s) 164, 270, 317
Bsp143I GATC 2 cut(s) 457, 511
Bsp19I CCATGG 1 cut(s) 543
BspACI CCGC 9 cut(s) 41, 59, 62, 126, 178, 190, 201, 485, 540
BspANI GGCC 1 cut(s) 102
BspCNI CTCAG 1 cut(s) 301
BspFNI CGCG 1 cut(s) 64
BspMAI CTGCAG 1 cut(s) 496
BspTI CTTAAG 1 cut(s) 410
BsrBI CCGCTC 1 cut(s) 128
BsrFI RCCGGY 1 cut(s) 180
BssAI RCCGGY 1 cut(s) 180
BssECI CCNNGG 2 cut(s) 97, 543
BssMI GATC 2 cut(s) 457, 511
BssNI GRCGYC 1 cut(s) 255
BssT1I CCWWGG 2 cut(s) 97, 543
Bst4CI ACNGT 3 cut(s) 19, 25, 208
Bst6I CTCTTC 1 cut(s) 342
BstACI GRCGYC 1 cut(s) 255
BstAFI CTTAAG 1 cut(s) 410
BstC8I GCNNGC 1 cut(s) 182
BstDEI CTNAG 1 cut(s) 309
BstDSI CCRYGG 1 cut(s) 543
BstF5I GGATG 2 cut(s) 93, 120
BstFNI CGCG 1 cut(s) 64
BstKTI GATC 2 cut(s) 460, 514
BstMAI GTCTC 1 cut(s) 333
BstMBI GATC 2 cut(s) 457, 511
BstMCI CGRYCG 1 cut(s) 450
BstMWI GCNNNNNNNGC 3 cut(s) 321, 449, 491
BstNSI RCATGY 1 cut(s) 406
BstSFI CTRYAG 1 cut(s) 492
BstSLI GKGCMC 2 cut(s) 164, 317
BstUI CGCG 1 cut(s) 64
BstV1I GCAGC 3 cut(s) 370, 478, 506
BsuRI GGCC 1 cut(s) 102
BtgI CCRYGG 1 cut(s) 543
BtgZI GCGATG 1 cut(s) 91
BtsCI GGATG 2 cut(s) 93, 120
Cac8I GCNNGC 1 cut(s) 182
Cfr10I RCCGGY 1 cut(s) 180
Cfr13I GGNCC 2 cut(s) 195, 352
CseI GACGC 2 cut(s) 58, 355
Csp6I GTAC 1 cut(s) 274
CviAII CATG 2 cut(s) 403, 544
CviJI RGCY 8 cut(s) 9, 102, 216, 268, 361, 409, 443, 497
CviKI_1 RGCY 8 cut(s) 9, 102, 216, 268, 361, 409, 443, 497
CviQI GTAC 1 cut(s) 274
DdeI CTNAG 1 cut(s) 309
DpnI GATC 2 cut(s) 459, 513
DpnII GATC 2 cut(s) 457, 511
Eam1104I CTCTTC 1 cut(s) 342
EarI CTCTTC 1 cut(s) 342
EciI GGCGGA 3 cut(s) 167, 190, 529
Ecl136II GAGCTC 1 cut(s) 268
Eco130I CCWWGG 2 cut(s) 97, 543
Eco24I GRGCYC 1 cut(s) 270
Eco47I GGWCC 2 cut(s) 195, 352
Eco53kI GAGCTC 1 cut(s) 268
EcoICRI GAGCTC 1 cut(s) 268
EcoRI GAATTC 1 cut(s) 89
EcoT14I CCWWGG 2 cut(s) 97, 543
EcoT38I GRGCYC 1 cut(s) 270
ErhI CCWWGG 2 cut(s) 97, 543
FaeI CATG 2 cut(s) 406, 547
FaiI YATR 3 cut(s) 398, 404, 545
FaqI GGGAC 1 cut(s) 194
FatI CATG 2 cut(s) 402, 543
FauI CCCGC 3 cut(s) 133, 197, 478
FblI GTMKAC 2 cut(s) 72, 135
Fnu4HI GCNGC 4 cut(s) 62, 359, 492, 495
FokI GGATG 2 cut(s) 80, 107
FriOI GRGCYC 1 cut(s) 270
Fsp4HI GCNGC 4 cut(s) 62, 359, 492, 495
FspBI CTAG 1 cut(s) 498
GluI GCNGC 4 cut(s) 62, 359, 492, 495
GsaI CCCAGC 2 cut(s) 56, 216
HaeIII GGCC 1 cut(s) 102
HapII CCGG 3 cut(s) 181, 193, 355
HgaI GACGC 2 cut(s) 58, 355
Hin1I GRCGYC 1 cut(s) 255
Hin1II CATG 2 cut(s) 406, 547
HincII GTYRAC 1 cut(s) 73
HindII GTYRAC 1 cut(s) 73
HpaII CCGG 3 cut(s) 181, 193, 355
HphI GGTGA 1 cut(s) 109
Hpy166II GTNNAC 2 cut(s) 73, 136
Hpy188I TCNGA 5 cut(s) 30, 265, 305, 462, 480
Hpy8I GTNNAC 2 cut(s) 73, 136
Hpy99I CGWCG 2 cut(s) 74, 257
HpyAV CCTTC 3 cut(s) 151, 174, 274
HpyCH4III ACNGT 3 cut(s) 19, 25, 208
HpyCH4IV ACGT 1 cut(s) 255
HpyCH4V TGCA 4 cut(s) 372, 396, 494, 517
HpyF10VI GCNNNNNNNGC 3 cut(s) 321, 449, 491
HpyF3I CTNAG 1 cut(s) 309
HpySE526I ACGT 1 cut(s) 255
Hsp92I GRCGYC 1 cut(s) 255
Hsp92II CATG 2 cut(s) 406, 547
KroI GCCGGC 1 cut(s) 180
KroNI GCCGGC 1 cut(s) 182
Kzo9I GATC 2 cut(s) 457, 511
LmnI GCTCC 1 cut(s) 265
LpnPI CCDG 7 cut(s) 66, 194, 206, 226, 290, 330, 368
Lsp1109I GCAGC 3 cut(s) 370, 478, 506
LweI GCATC 1 cut(s) 523
MaeI CTAG 1 cut(s) 498
MaeII ACGT 1 cut(s) 255
MaeIII GTNAC 1 cut(s) 19
MalI GATC 2 cut(s) 459, 513
MbiI CCGCTC 1 cut(s) 128
MboI GATC 2 cut(s) 457, 511
MboII GAAGA 3 cut(s) 136, 139, 359
MhlI GDGCHC 3 cut(s) 164, 270, 317
MluCI AATT 1 cut(s) 89
MmeI TCCRAC 2 cut(s) 192, 447
MnlI CCTC 5 cut(s) 95, 113, 208, 343, 494
MreI CGCCGGCG 1 cut(s) 180
MroNI GCCGGC 1 cut(s) 180
MseI TTAA 2 cut(s) 294, 411
MslI CAYNNNNRTG 1 cut(s) 401
MspCI CTTAAG 1 cut(s) 410
MspI CCGG 3 cut(s) 181, 193, 355
MvnI CGCG 1 cut(s) 64
MwoI GCNNNNNNNGC 3 cut(s) 321, 449, 491
NaeI GCCGGC 1 cut(s) 182
NcoI CCATGG 1 cut(s) 543
NdeII GATC 2 cut(s) 457, 511
NgoMIV GCCGGC 1 cut(s) 180
NlaIII CATG 2 cut(s) 406, 547
NspI RCATGY 1 cut(s) 406
PciI ACATGT 1 cut(s) 402
PdiI GCCGGC 1 cut(s) 182
PkrI GCNGC 4 cut(s) 63, 360, 493, 496
PscI ACATGT 1 cut(s) 402
Psp124BI GAGCTC 1 cut(s) 270
PspFI CCCAGC 2 cut(s) 52, 212
PspPI GGNCC 2 cut(s) 195, 352
PstI CTGCAG 1 cut(s) 496
RsaI GTAC 1 cut(s) 275
RsaNI GTAC 1 cut(s) 274
RseI CAYNNNNRTG 1 cut(s) 401
SacI GAGCTC 1 cut(s) 270
SalI GTCGAC 1 cut(s) 71
SaqAI TTAA 2 cut(s) 294, 411
SatI GCNGC 4 cut(s) 62, 359, 492, 495
Sau3AI GATC 2 cut(s) 457, 511
Sau96I GGNCC 2 cut(s) 195, 352
SduI GDGCHC 3 cut(s) 164, 270, 317
SfaNI GCATC 1 cut(s) 523
SfcI CTRYAG 1 cut(s) 492
SgrAI CRCCGGYG 1 cut(s) 180
SinI GGWCC 2 cut(s) 195, 352
SmiMI CAYNNNNRTG 1 cut(s) 401
SmlI CTYRAG 2 cut(s) 269, 410
SmoI CTYRAG 2 cut(s) 269, 410
Sse9I AATT 1 cut(s) 89
SsiI CCGC 9 cut(s) 41, 59, 62, 126, 178, 190, 201, 485, 540
SspMI CTAG 1 cut(s) 498
SstI GAGCTC 1 cut(s) 270
StyI CCWWGG 2 cut(s) 97, 543
TaaI ACNGT 3 cut(s) 19, 25, 208
TaiI ACGT 1 cut(s) 258
TaqI TCGA 3 cut(s) 72, 87, 439
TaqII GACCGA 1 cut(s) 436
TasI AATT 1 cut(s) 89
TauI GCSGC 1 cut(s) 64
Tru1I TTAA 2 cut(s) 294, 411
Tru9I TTAA 2 cut(s) 294, 411
TseI GCWGC 3 cut(s) 358, 491, 494
TspDTI ATGAA 1 cut(s) 82
Vha464I CTTAAG 1 cut(s) 410
VpaK11BI GGWCC 2 cut(s) 195, 352
XapI RAATTY 1 cut(s) 89
XceI RCATGY 1 cut(s) 406
XmiI GTMKAC 2 cut(s) 72, 135
XspI CTAG 1 cut(s) 498
ZraI GACGTC 1 cut(s) 256
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.