Rh3DG154100

RING-H2 finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0012772
Physical Location & Seq
Reverse (-)
13244661 .. 13246309
1649 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG154100.1

Sequence Viewer

Length: 621 bp
ATGGAGAGCTCACCGGCCATCCTAAACAAACCACCCCCGCCCTTCCCGGCGCCACCGCGCAGCGTCGACTTCTCCGCCCTCGAGTTCATCCTCGGCCTCCTCGCCGTCATCACCGTCCCTGCCTTAATCTACGCTGTCTTCTTCACCATCAAGTGTCCTCCCAACCTTTTCCGCCATCGACCCCGCCGGAGCTCCACCACCGCCCCCGCCGACCCTGCCCATGCCCGGCCCGAGGTGATTTCCGACCTCAAATACCAGAAGGACGCCCACGTCAAGGAAATCGGGACCGAGTGCCCGGTTTGCTTATCGGTCTTTGCCGACGGCGAAGAAGTGAGGCAACTCAGTGTGTGCAAGCACTCGTTTCATGGCACGTGCATTAACATGTGGCTCAAGGATCACTCGAATTGCCCGATATGCCGCGCGAACATCGCCGTTGATCGGAGTAGTAGTAACAGCAGAACGGCGGCGTCCGGTAGAGAATATCGGGATGATCTGCAACAAGGTTTGCCCGATGCTTCCGGCTTGTTTCAGAAACTTACCGACAGAGTTTTAAATTTTTTTCTTGTCAAAACAAATGCAAAGGGTAAGAACAATGATGACATACAAATAGTGAGCGGGTGA

Protein Analysis

206

Amino Acids

22.69

Weight (kDa)

8.48

Isoelectric Point (pI)

53.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 97 - 140 8.8e-13 Ring finger domain
zf-C3HC4_2 PF13923 97 - 139 3.1e-09 Zinc finger, C3HC4 type (RING finger)
zf-RING_11 PF17123 97 - 126 1.9e-08 RING-like zinc finger
zf-rbx1 PF12678 98 - 140 9.7e-08 RING-H2 zinc finger domain
zf-C3HC4 PF00097 98 - 139 6.5e-07 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012772)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G37580
fragaria_vesca FvH4_6g14030
malus_domestica MD12G1078700.v1.1
prunus_persica Prupe.6G253000_v2.0.a1
pyrus_communis pycom04g11330 pycom12g13480
rosa_chinensis RchiOBHm_Chr3g0466911
rosa_laevigata RLG00000020757 RLG00000024546
rosa_multiflora Rmu_co8052150.1_g000001 Rmu_sc0001193.1_g000017
rosa_roxburghii Rroxscaffold_6G00414070
rosa_rugosa Rorug03G0084200
rosa_samantha Rh3AG132100 Rh3BG153200 Rh3CG153200 Rh3DG154100
rosa_wichuraiana Rw3G012470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 269
AccB1I GGYRCC 1 cut(s) 49
AccBSI CCGCTC 1 cut(s) 615
AccI GTMKAC 1 cut(s) 66
AccII CGCG 3 cut(s) 58, 420, 422
AclWI GGATC 1 cut(s) 402
AcoI YGGCCR 1 cut(s) 15
AcsI RAATTY 1 cut(s) 553
AcvI CACGTG 1 cut(s) 372
AcyI GRCGYC 3 cut(s) 50, 264, 467
AfiI CCNNNNNNNGG 4 cut(s) 225, 232, 274, 438
AflIII ACRYGT 1 cut(s) 381
AjiI CACGTC 1 cut(s) 271
AluBI AGCT 2 cut(s) 9, 192
AluI AGCT 2 cut(s) 9, 192
Alw21I GWGCWC 2 cut(s) 11, 194
AlwI GGATC 1 cut(s) 402
Ama87I CYCGRG 2 cut(s) 80, 230
AoxI GGCC 3 cut(s) 15, 94, 227
ApeKI GCWGC 1 cut(s) 60
ApoI RAATTY 1 cut(s) 553
AspLEI GCGC 3 cut(s) 52, 60, 422
AspS9I GGNCC 2 cut(s) 228, 285
AsuC2I CCSGG 3 cut(s) 47, 226, 296
AsuHPI GGTGA 4 cut(s) 3, 103, 136, 247
AvaI CYCGRG 2 cut(s) 80, 230
AvaII GGWCC 1 cut(s) 285
BaeGI GKGCMC 1 cut(s) 296
BanI GGYRCC 1 cut(s) 49
BanII GRGCYC 2 cut(s) 11, 194
BbrPI CACGTG 1 cut(s) 372
BbsI GAAGAC 1 cut(s) 130
Bbv12I GWGCWC 2 cut(s) 11, 194
BbvI GCAGC 1 cut(s) 72
BccI CCATC 3 cut(s) 26, 155, 183
BceAI ACGGC 4 cut(s) 89, 337, 416, 477
BcnI CCSGG 3 cut(s) 47, 226, 296
BfoI RGCGCY 1 cut(s) 53
BisI GCNGC 3 cut(s) 61, 418, 465
BlsI GCNGC 3 cut(s) 62, 419, 466
Bme1390I CCNGG 3 cut(s) 47, 226, 296
Bme18I GGWCC 1 cut(s) 285
BmeT110I CYCGRG 2 cut(s) 80, 230
BmgBI CACGTC 1 cut(s) 271
BmgT120I GGNCC 2 cut(s) 228, 285
BmiI GGNNCC 2 cut(s) 51, 286
BmrFI CCNGG 3 cut(s) 47, 226, 296
BmsI GCATC 1 cut(s) 502
BpiI GAAGAC 1 cut(s) 130
BpuEI CTTGAG 1 cut(s) 374
BpuMI CCSGG 3 cut(s) 47, 226, 296
BsaAI YACGTR 1 cut(s) 372
BsaHI GRCGYC 3 cut(s) 50, 264, 467
BsaJI CCNNGG 2 cut(s) 91, 231
BsaWI WCCGGW 1 cut(s) 470
BsaXI ACNNNNNCTCC 2 cut(s) 433, 463
Bsc4I CCNNNNNNNGG 4 cut(s) 225, 232, 274, 438
Bse118I RCCGGY 1 cut(s) 13
BseDI CCNNGG 2 cut(s) 91, 231
BseGI GGATG 3 cut(s) 18, 87, 493
BseLI CCNNNNNNNGG 4 cut(s) 225, 232, 274, 438
BseMII CTCAG 1 cut(s) 355
BseRI GAGGAG 1 cut(s) 89
BseSI GKGCMC 1 cut(s) 296
BseXI GCAGC 1 cut(s) 72
Bsh1236I CGCG 3 cut(s) 58, 420, 422
BshFI GGCC 3 cut(s) 17, 96, 229
BshNI GGYRCC 1 cut(s) 49
BsiHKAI GWGCWC 2 cut(s) 11, 194
BsiHKCI CYCGRG 2 cut(s) 80, 230
BsiSI CCGG 7 cut(s) 14, 47, 187, 226, 296, 471, 519
BslFI GGGAC 2 cut(s) 101, 298
BslI CCNNNNNNNGG 4 cut(s) 225, 232, 274, 438
BsmFI GGGAC 2 cut(s) 101, 298
BsnI GGCC 3 cut(s) 17, 96, 229
BsoBI CYCGRG 2 cut(s) 80, 230
Bsp1286I GDGCHC 3 cut(s) 11, 194, 296
Bsp143I GATC 3 cut(s) 394, 436, 490
BspANI GGCC 3 cut(s) 17, 96, 229
BspCNI CTCAG 1 cut(s) 354
BspFNI CGCG 3 cut(s) 58, 420, 422
BspLI GGNNCC 2 cut(s) 51, 286
BspPI GGATC 1 cut(s) 402
BspT107I GGYRCC 1 cut(s) 49
BsrBI CCGCTC 1 cut(s) 615
BsrFI RCCGGY 1 cut(s) 13
BssAI RCCGGY 1 cut(s) 13
BssECI CCNNGG 2 cut(s) 91, 231
BssMI GATC 3 cut(s) 394, 436, 490
BssNI GRCGYC 3 cut(s) 50, 264, 467
Bst4CI ACNGT 1 cut(s) 115
BstACI GRCGYC 3 cut(s) 50, 264, 467
BstBAI YACGTR 1 cut(s) 372
BstC8I GCNNGC 1 cut(s) 353
BstDEI CTNAG 1 cut(s) 341
BstF5I GGATG 3 cut(s) 18, 87, 493
BstFNI CGCG 3 cut(s) 58, 420, 422
BstH2I RGCGCY 1 cut(s) 53
BstHHI GCGC 3 cut(s) 52, 60, 422
BstKTI GATC 3 cut(s) 397, 439, 493
BstMBI GATC 3 cut(s) 394, 436, 490
BstMWI GCNNNNNNNGC 4 cut(s) 215, 300, 414, 428
BstNSI RCATGY 1 cut(s) 385
BstSCI CCNGG 3 cut(s) 45, 224, 294
BstSLI GKGCMC 1 cut(s) 296
BstUI CGCG 3 cut(s) 58, 420, 422
BstV1I GCAGC 1 cut(s) 72
BstV2I GAAGAC 1 cut(s) 130
BsuRI GGCC 3 cut(s) 17, 96, 229
BtgZI GCGATG 1 cut(s) 412
BtrI CACGTC 1 cut(s) 271
BtsCI GGATG 3 cut(s) 18, 87, 493
BtsIMutI CAGTG 1 cut(s) 349
Cac8I GCNNGC 1 cut(s) 353
CfoI GCGC 3 cut(s) 52, 60, 422
Cfr10I RCCGGY 1 cut(s) 13
Cfr13I GGNCC 2 cut(s) 228, 285
CseI GACGC 3 cut(s) 52, 272, 456
CviAII CATG 3 cut(s) 221, 365, 382
CviJI RGCY 7 cut(s) 9, 17, 96, 192, 229, 388, 522
CviKI_1 RGCY 7 cut(s) 9, 17, 96, 192, 229, 388, 522
DdeI CTNAG 1 cut(s) 341
DinI GGCGCC 1 cut(s) 51
DpnI GATC 3 cut(s) 396, 438, 492
DpnII GATC 3 cut(s) 394, 436, 490
DraI TTTAAA 1 cut(s) 552
DrdI GACNNNNNNGTC 1 cut(s) 269
DseDI GACNNNNNNGTC 1 cut(s) 269
EaeI YGGCCR 1 cut(s) 15
EciI GGCGGA 2 cut(s) 64, 161
Ecl136II GAGCTC 2 cut(s) 9, 192
Eco24I GRGCYC 2 cut(s) 11, 194
Eco47I GGWCC 1 cut(s) 285
Eco53kI GAGCTC 2 cut(s) 9, 192
Eco72I CACGTG 1 cut(s) 372
Eco88I CYCGRG 2 cut(s) 80, 230
EcoICRI GAGCTC 2 cut(s) 9, 192
EcoT38I GRGCYC 2 cut(s) 11, 194
EgeI GGCGCC 1 cut(s) 51
EheI GGCGCC 1 cut(s) 51
FaeI CATG 3 cut(s) 224, 368, 385
FaiI YATR 5 cut(s) 222, 366, 383, 415, 602
FaqI GGGAC 2 cut(s) 101, 298
FatI CATG 3 cut(s) 220, 364, 381
FauI CCCGC 4 cut(s) 45, 191, 214, 608
FblI GTMKAC 1 cut(s) 66
Fnu4HI GCNGC 3 cut(s) 61, 418, 465
FokI GGATG 3 cut(s) 5, 74, 500
FriOI GRGCYC 2 cut(s) 11, 194
Fsp4HI GCNGC 3 cut(s) 61, 418, 465
GlaI GCGC 3 cut(s) 51, 59, 421
GluI GCNGC 3 cut(s) 61, 418, 465
HaeII RGCGCY 1 cut(s) 53
HaeIII GGCC 3 cut(s) 17, 96, 229
HapII CCGG 7 cut(s) 14, 47, 187, 226, 296, 471, 519
HgaI GACGC 3 cut(s) 52, 272, 456
HhaI GCGC 3 cut(s) 52, 60, 422
Hin1I GRCGYC 3 cut(s) 50, 264, 467
Hin1II CATG 3 cut(s) 224, 368, 385
Hin6I GCGC 3 cut(s) 50, 58, 420
HinP1I GCGC 3 cut(s) 50, 58, 420
HincII GTYRAC 1 cut(s) 67
HindII GTYRAC 1 cut(s) 67
HpaII CCGG 7 cut(s) 14, 47, 187, 226, 296, 471, 519
HphI GGTGA 4 cut(s) 3, 103, 136, 247
Hpy166II GTNNAC 1 cut(s) 67
Hpy188I TCNGA 3 cut(s) 244, 441, 531
Hpy188III TCNNGA 2 cut(s) 283, 485
Hpy8I GTNNAC 1 cut(s) 67
Hpy99I CGWCG 2 cut(s) 68, 323
HpyAV CCTTC 2 cut(s) 52, 253
HpyCH4III ACNGT 1 cut(s) 115
HpyCH4IV ACGT 2 cut(s) 270, 371
HpyCH4V TGCA 4 cut(s) 351, 375, 496, 578
HpyF10VI GCNNNNNNNGC 4 cut(s) 215, 300, 414, 428
HpyF3I CTNAG 1 cut(s) 341
HpySE526I ACGT 2 cut(s) 270, 371
Hsp92I GRCGYC 3 cut(s) 50, 264, 467
Hsp92II CATG 3 cut(s) 224, 368, 385
HspAI GCGC 3 cut(s) 50, 58, 420
KasI GGCGCC 1 cut(s) 49
Kzo9I GATC 3 cut(s) 394, 436, 490
LmnI GCTCC 2 cut(s) 189, 197
Lsp1109I GCAGC 1 cut(s) 72
LweI GCATC 1 cut(s) 502
MaeII ACGT 2 cut(s) 270, 371
MaeIII GTNAC 1 cut(s) 449
MalI GATC 3 cut(s) 396, 438, 492
MbiI CCGCTC 1 cut(s) 615
MboI GATC 3 cut(s) 394, 436, 490
MboII GAAGA 3 cut(s) 130, 133, 338
MhlI GDGCHC 3 cut(s) 11, 194, 296
MluCI AATT 2 cut(s) 403, 553
Mly113I GGCGCC 1 cut(s) 50
MmeI TCCRAC 1 cut(s) 267
MnlI CCTC 8 cut(s) 89, 101, 107, 110, 168, 226, 257, 327
MseI TTAA 3 cut(s) 125, 378, 551
MslI CAYNNNNRTG 1 cut(s) 380
MspI CCGG 7 cut(s) 14, 47, 187, 226, 296, 471, 519
MspR9I CCNGG 3 cut(s) 47, 226, 296
MvnI CGCG 3 cut(s) 58, 420, 422
MwoI GCNNNNNNNGC 4 cut(s) 215, 300, 414, 428
NarI GGCGCC 1 cut(s) 50
NciI CCSGG 3 cut(s) 47, 226, 296
NdeII GATC 3 cut(s) 394, 436, 490
NlaIII CATG 3 cut(s) 224, 368, 385
NlaIV GGNNCC 2 cut(s) 51, 286
NmeAIII GCCGAG 1 cut(s) 72
NspI RCATGY 1 cut(s) 385
PaeR7I CTCGAG 1 cut(s) 80
PciI ACATGT 1 cut(s) 381
PcsI WCGNNNNNNNCGW 1 cut(s) 407
PkrI GCNGC 3 cut(s) 62, 419, 466
PluTI GGCGCC 1 cut(s) 53
PmaCI CACGTG 1 cut(s) 372
PmlI CACGTG 1 cut(s) 372
Ppu21I YACGTR 1 cut(s) 372
PscI ACATGT 1 cut(s) 381
Psp124BI GAGCTC 2 cut(s) 11, 194
PspCI CACGTG 1 cut(s) 372
PspN4I GGNNCC 2 cut(s) 51, 286
PspPI GGNCC 2 cut(s) 228, 285
PspXI VCTCGAGB 1 cut(s) 80
RseI CAYNNNNRTG 1 cut(s) 380
SacI GAGCTC 2 cut(s) 11, 194
SalI GTCGAC 1 cut(s) 65
SaqAI TTAA 3 cut(s) 125, 378, 551
SatI GCNGC 3 cut(s) 61, 418, 465
Sau3AI GATC 3 cut(s) 394, 436, 490
Sau96I GGNCC 2 cut(s) 228, 285
ScrFI CCNGG 3 cut(s) 47, 226, 296
SduI GDGCHC 3 cut(s) 11, 194, 296
SetI ASST 8 cut(s) 11, 168, 194, 237, 249, 273, 374, 505
SfaNI GCATC 1 cut(s) 502
SfoI GGCGCC 1 cut(s) 51
Sfr274I CTCGAG 1 cut(s) 80
SinI GGWCC 1 cut(s) 285
SlaI CTCGAG 1 cut(s) 80
SmiMI CAYNNNNRTG 1 cut(s) 380
SmlI CTYRAG 2 cut(s) 80, 389
SmoI CTYRAG 2 cut(s) 80, 389
Sse9I AATT 2 cut(s) 403, 553
SspDI GGCGCC 1 cut(s) 49
SstI GAGCTC 2 cut(s) 11, 194
StyD4I CCNGG 3 cut(s) 45, 224, 294
TaaI ACNGT 1 cut(s) 115
TaiI ACGT 2 cut(s) 273, 374
TaqI TCGA 4 cut(s) 66, 81, 178, 401
TaqII GACCGA 2 cut(s) 298, 302
TasI AATT 2 cut(s) 403, 553
TauI GCSGC 2 cut(s) 420, 467
Tru1I TTAA 3 cut(s) 125, 378, 551
Tru9I TTAA 3 cut(s) 125, 378, 551
TscAI CASTG 1 cut(s) 349
TseI GCWGC 1 cut(s) 60
TspDTI ATGAA 2 cut(s) 76, 353
TspRI CASTG 1 cut(s) 349
VpaK11BI GGWCC 1 cut(s) 285
XapI RAATTY 1 cut(s) 553
XceI RCATGY 1 cut(s) 385
XhoI CTCGAG 1 cut(s) 80
XmiI GTMKAC 1 cut(s) 66
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.