FvH4_6g14691

ankyrin repeat-containing protein At5g02620-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0004020
Physical Location & Seq
Forward (+)
9048178 .. 9051822
3645 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g14691.t4

Sequence Viewer

Length: 1632 bp
ATGGAAGCACCTGTGAGGCAGCAAAGCTTCTGTGAGAAGAAGATGACGAAGCAGTTGACAGGGAAGCGGGAAGATGCACCCTTGCATTTGGCAGCAAGAACAGGAAATTTGGGATTGGTGTTAGAGATCATCTCAAATTGTGGGGAGGCAGAGTTGGATGAAGTGTTGATGAAGCAGAATCACTCGGGTGAAATCGCCTTGTATGTGGCGGCTGAGTGTGGCTATGTTGATGTGGTGAAGGAAATGATGAAGTACTGTGATGCTGGTTTAGCTAGTATCAAAGCTAGAAATGGGTTTGATGCATTTCACATTGCTTCCAAGCAAGGCCACTTAGAGGTATTGAAGGTACTCATGGAGGCAATACCAGAACTTTCGATGACGGTTGATCAAACAAACACCACAGCACTGCACACTGCAGCAGCGCAGGGTCACATTGAAGTAGTAAATTTCCTTTTGGAGAATGATTGCCGCTTGGTATCCATAGCACGAAGCAATGCAAAAACTGCCTTTCATTCTGCAGCAAGAAATGGGCATTTAGATGTCATCAAGGCACTCTTGAGAAAAGAACCTGAAACTGCAACGAGGAAGGATAAGAAGGGGCAGACAGCTCTCCATATGGCAGTTAAGGGACAGAATGTTGAGCTGGTGGATGAGCTAGCAAAAGCGGATCCTTCTATGATAAACATGGTGGATAACAAGGGTAACACTGCCTTGCATATAGCAACCCGGAAGGGTAGAGCTCAGATTGTTCAGCAGCTGTTGGGTTACAAGGGAATTGCCACGACTATCATTAACAAATCTGGTGAAACTGTTCTTGACACTGCTCAGAAAAATGGGCAATCTGTAATTGCTGCCATTCTAGAAGAGCAAGGCATTCAAAGTGCCAAATCCATGAGACCACCAACCATAATGGCAAACCGAGAGCTCAAACAAACTGTCAGTGACATAAAACATGAGGTGCATGACCAGATCAAGCAAACACGGCATACACGAAAGCAAGTGCATGGTATGGTAAAACGGCTCAGCAAAATGCACTCGGATGGGCTTAACAATGCAATAAACTCCACCACATTTGTGGCTGTCCTCATTGCCACCGTTGCCTTTGCTGCAATTTTCACAGTCCCAGGCCAATACCCCAAAGAAGAGGATAATCTGCCACCTGAATACTCTCCTGGAGAAGCGAACATTGCTCCAAAAGCCGAGTTTATAGTCTTCTTCGTCTTTGATTCTTTTGCACTCTTCATATCTTTGGCTGTAGTGGTGGTTCAAACTTCTATTGTTGTTATAGAGAGGAAGCAAAAAGAGCAGTTGATGTCAATCATAAACAAGCTAATGTGGATGGCTTGTGTGATGGTTTCGGTTGCATTTCTTGCACTGTCTTATGTGATTGTCGGAGAGAAAGAGAAATGGTTGGCTGTTGGAGTAACAGGTATAGGTACAGTGATTTTGGGAATGACATTGGGAACAATGTGTTATTGGATGGTTGTGCAGAGACTTGAGGCTTCAAAACTTCGTAGCAGCACTCGAAGATCATCAATGAGCACTAGGTCACATTCTGGGTCTTTTTCAGTGATGTCAGATTCTGAGCTCATCAACACTGAGTTTAAAAAGAAGAGAGTGTATGCAATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005886 GO:0012505 GO:0016020 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 Ank_3 Ank_4 Ank_5 PGG

Protein Analysis

544

Amino Acids

59.73

Weight (kDa)

9.0

Isoelectric Point (pI)

40.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 18 - 87 9.3e-06 Ankyrin repeats (3 copies)
Ank_2 PF12796 95 - 157 3.5e-11 Ankyrin repeats (3 copies)
Ank_4 PF13637 103 - 152 2.8e-07 Ankyrin repeats (many copies)
Ank_4 PF13637 136 - 186 2.9e-08 Ankyrin repeats (many copies)
Ank_2 PF12796 165 - 224 1.1e-10 Ankyrin repeats (3 copies)
Ank_4 PF13637 171 - 216 6.5e-07 Ankyrin repeats (many copies)
Ank_5 PF13857 195 - 241 4.1e-08 Ankyrin repeats (many copies)
Ank_4 PF13637 204 - 253 5.5e-07 Ankyrin repeats (many copies)
Ank_2 PF12796 213 - 292 7.2e-09 Ankyrin repeats (3 copies)
PGG PF13962 348 - 462 2.8e-29 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 67, 209, 469, 665
AclWI GGATC 2 cut(s) 662, 675
AcsI RAATTY 2 cut(s) 106, 445
AfaI GTAC 3 cut(s) 254, 348, 1438
AfiI CCNNNNNNNGG 1 cut(s) 334
AgsI TTSAA 5 cut(s) 343, 437, 878, 1268, 1506
AjnI CCWGG 2 cut(s) 1123, 1171
AleI CACNNNNGTG 2 cut(s) 186, 1073
Alw21I GWGCWC 4 cut(s) 742, 927, 1544, 1590
Alw26I GTCTC 2 cut(s) 889, 1486
AlwI GGATC 2 cut(s) 662, 675
AlwNI CAGNNNCTG 2 cut(s) 757, 1583
Ama87I CYCGRG 1 cut(s) 184
AoxI GGCC 2 cut(s) 325, 1126
ApeKI GCWGC 9 cut(s) 19, 92, 416, 419, 518, 754, 851, 1106, 1518
ApoI RAATTY 2 cut(s) 106, 445
ArsI GACNNNNNNTTYG 2 cut(s) 921, 953
Asp700I GAANNNNTTC 1 cut(s) 810
AspLEI GCGC 1 cut(s) 424
AsuC2I CCSGG 1 cut(s) 727
AsuHPI GGTGA 3 cut(s) 200, 247, 815
AsuNHI GCTAGC 1 cut(s) 655
AvaI CYCGRG 1 cut(s) 184
BamHI GGATCC 1 cut(s) 667
BanII GRGCYC 3 cut(s) 742, 927, 1590
BbsI GAAGAC 1 cut(s) 1204
Bbv12I GWGCWC 4 cut(s) 742, 927, 1544, 1590
BbvI GCAGC 9 cut(s) 31, 104, 428, 431, 530, 766, 838, 1093, 1530
BccI CCATC 4 cut(s) 1034, 1333, 1345, 1474
BceAI ACGGC 2 cut(s) 998, 1034
BciT130I CCWGG 2 cut(s) 1125, 1173
BciVI GTATCC 1 cut(s) 487
BclI TGATCA 1 cut(s) 385
BcnI CCSGG 1 cut(s) 727
BcoDI GTCTC 2 cut(s) 889, 1486
BfaI CTAG 5 cut(s) 273, 285, 656, 860, 1545
BfmI CTRYAG 3 cut(s) 414, 516, 1254
BfuI GTATCC 1 cut(s) 487
BlpI GCTNAGC 1 cut(s) 1022
BmcAI AGTACT 1 cut(s) 254
Bme1390I CCNGG 3 cut(s) 727, 1125, 1173
BmeT110I CYCGRG 1 cut(s) 184
BmiI GGNNCC 1 cut(s) 669
BmrFI CCNGG 3 cut(s) 727, 1125, 1173
BmsI GCATC 3 cut(s) 64, 250, 289
BmtI GCTAGC 1 cut(s) 659
BpiI GAAGAC 1 cut(s) 1204
BplI GAGNNNNNCTC 2 cut(s) 116, 148
BpmI CTGGAG 1 cut(s) 1194
Bpu1102I GCTNAGC 1 cut(s) 1022
BpuEI CTTGAG 2 cut(s) 577, 1517
BpuMI CCSGG 1 cut(s) 727
BsaBI GATNNNNATC 1 cut(s) 1316
BsaI GGTCTC 1 cut(s) 889
BsaJI CCNNGG 1 cut(s) 1123
BsaXI ACNNNNNCTCC 2 cut(s) 137, 167
Bsc4I CCNNNNNNNGG 1 cut(s) 334
Bse3DI GCAATG 4 cut(s) 309, 499, 1086, 1185
Bse8I GATNNNNATC 1 cut(s) 1316
BseBI CCWGG 2 cut(s) 1125, 1173
BseDI CCNNGG 1 cut(s) 1123
BseGI GGATG 5 cut(s) 163, 655, 1045, 1344, 1485
BseJI GATNNNNATC 1 cut(s) 1316
BseLI CCNNNNNNNGG 1 cut(s) 334
BseMI GCAATG 4 cut(s) 309, 499, 1086, 1185
BseMII CTCAG 6 cut(s) 204, 755, 839, 1036, 1575, 1590
BseXI GCAGC 9 cut(s) 31, 104, 428, 431, 530, 766, 838, 1093, 1530
BsgI GTGCAG 2 cut(s) 392, 1508
BshFI GGCC 2 cut(s) 327, 1128
BsiHKAI GWGCWC 4 cut(s) 742, 927, 1544, 1590
BsiHKCI CYCGRG 1 cut(s) 184
BsiSI CCGG 1 cut(s) 727
BslFI GGGAC 2 cut(s) 642, 1106
BslI CCNNNNNNNGG 1 cut(s) 334
BsmAI GTCTC 2 cut(s) 889, 1486
BsmFI GGGAC 2 cut(s) 642, 1106
BsmI GAATGC 1 cut(s) 873
BsnI GGCC 2 cut(s) 327, 1128
Bso31I GGTCTC 1 cut(s) 889
BsoBI CYCGRG 1 cut(s) 184
Bsp1286I GDGCHC 4 cut(s) 742, 927, 1544, 1590
Bsp143I GATC 5 cut(s) 126, 385, 667, 969, 1529
Bsp1720I GCTNAGC 1 cut(s) 1022
BspACI CCGC 4 cut(s) 67, 209, 469, 665
BspANI GGCC 2 cut(s) 327, 1128
BspCNI CTCAG 6 cut(s) 205, 754, 838, 1035, 1576, 1591
BspLI GGNNCC 1 cut(s) 669
BspMAI CTGCAG 2 cut(s) 418, 520
BspOI GCTAGC 1 cut(s) 659
BspPI GGATC 2 cut(s) 662, 675
BspQI GCTCTTC 1 cut(s) 858
BspTNI GGTCTC 1 cut(s) 889
BsrDI GCAATG 4 cut(s) 309, 499, 1086, 1185
BssECI CCNNGG 1 cut(s) 1123
BssMI GATC 5 cut(s) 126, 385, 667, 969, 1529
Bst2UI CCWGG 2 cut(s) 1125, 1173
Bst4CI ACNGT 8 cut(s) 257, 382, 811, 937, 1096, 1120, 1377, 1441
Bst6I CTCTTC 4 cut(s) 858, 1137, 1244, 1607
BstAPI GCANNNNNTGC 2 cut(s) 503, 1370
BstC8I GCNNGC 1 cut(s) 657
BstDEI CTNAG 7 cut(s) 213, 331, 741, 825, 1022, 1584, 1599
BstF5I GGATG 5 cut(s) 163, 655, 1045, 1344, 1485
BstHHI GCGC 1 cut(s) 424
BstKTI GATC 5 cut(s) 129, 388, 670, 972, 1532
BstMAI GTCTC 2 cut(s) 889, 1486
BstMBI GATC 5 cut(s) 126, 385, 667, 969, 1529
BstMWI GCNNNNNNNGC 9 cut(s) 269, 503, 982, 1097, 1106, 1187, 1196, 1303, 1370
BstNI CCWGG 2 cut(s) 1125, 1173
BstSCI CCNGG 3 cut(s) 725, 1123, 1171
BstSFI CTRYAG 3 cut(s) 414, 516, 1254
BstV1I GCAGC 9 cut(s) 31, 104, 428, 431, 530, 766, 838, 1093, 1530
BstV2I GAAGAC 1 cut(s) 1204
BstX2I RGATCY 1 cut(s) 667
BstXI CCANNNNNNTGG 1 cut(s) 1075
BstYI RGATCY 1 cut(s) 667
BsuI GTATCC 1 cut(s) 487
BsuRI GGCC 2 cut(s) 327, 1128
BtsCI GGATG 5 cut(s) 163, 655, 1045, 1344, 1485
BtsI GCAGTG 4 cut(s) 404, 411, 705, 819
BtsIMutI CAGTG 9 cut(s) 404, 411, 705, 819, 946, 1373, 1446, 1575, 1596
Cac8I GCNNGC 1 cut(s) 657
CaiI CAGNNNCTG 2 cut(s) 757, 1583
CfoI GCGC 1 cut(s) 424
Csp6I GTAC 3 cut(s) 253, 347, 1437
CspCI CAANNNNNGTGG 2 cut(s) 1054, 1089
CviAII CATG 6 cut(s) 352, 685, 892, 953, 962, 1004
CviQI GTAC 3 cut(s) 253, 347, 1437
DdeI CTNAG 7 cut(s) 213, 331, 741, 825, 1022, 1584, 1599
DpnI GATC 5 cut(s) 128, 387, 669, 971, 1531
DpnII GATC 5 cut(s) 126, 385, 667, 969, 1529
DraI TTTAAA 1 cut(s) 1606
Eam1104I CTCTTC 4 cut(s) 858, 1137, 1244, 1607
EarI CTCTTC 4 cut(s) 858, 1137, 1244, 1607
Ecl136II GAGCTC 3 cut(s) 740, 925, 1588
Eco24I GRGCYC 3 cut(s) 742, 927, 1590
Eco31I GGTCTC 1 cut(s) 889
Eco53kI GAGCTC 3 cut(s) 740, 925, 1588
Eco88I CYCGRG 1 cut(s) 184
EcoICRI GAGCTC 3 cut(s) 740, 925, 1588
EcoRII CCWGG 2 cut(s) 1123, 1171
EcoT22I ATGCAT 1 cut(s) 304
EcoT38I GRGCYC 3 cut(s) 742, 927, 1590
FaeI CATG 6 cut(s) 355, 688, 895, 956, 965, 1007
FalI AAGNNNNNCTT 2 cut(s) 539, 571
FaqI GGGAC 2 cut(s) 642, 1106
FatI CATG 6 cut(s) 351, 684, 891, 952, 961, 1003
FauI CCCGC 1 cut(s) 60
FauNDI CATATG 1 cut(s) 615
FbaI TGATCA 1 cut(s) 385
FokI GGATG 5 cut(s) 170, 662, 1052, 1351, 1492
FriOI GRGCYC 3 cut(s) 742, 927, 1590
FspBI CTAG 5 cut(s) 273, 285, 656, 860, 1545
GlaI GCGC 1 cut(s) 423
GsuI CTGGAG 1 cut(s) 1194
HaeIII GGCC 2 cut(s) 327, 1128
HapII CCGG 1 cut(s) 727
HhaI GCGC 1 cut(s) 424
Hin1II CATG 6 cut(s) 355, 688, 895, 956, 965, 1007
Hin6I GCGC 1 cut(s) 422
HinP1I GCGC 1 cut(s) 422
HincII GTYRAC 1 cut(s) 57
HindII GTYRAC 1 cut(s) 57
HindIII AAGCTT 1 cut(s) 25
HinfI GANTC 3 cut(s) 178, 1226, 1580
HpaII CCGG 1 cut(s) 727
HphI GGTGA 3 cut(s) 200, 247, 815
Hpy166II GTNNAC 1 cut(s) 57
Hpy188I TCNGA 6 cut(s) 744, 828, 1039, 1394, 1579, 1585
Hpy188III TCNNGA 3 cut(s) 556, 815, 860
Hpy8I GTNNAC 1 cut(s) 57
HpyAV CCTTC 6 cut(s) 232, 337, 580, 589, 681, 724
HpyCH4III ACNGT 8 cut(s) 257, 382, 811, 937, 1096, 1120, 1377, 1441
HpyF10VI GCNNNNNNNGC 9 cut(s) 269, 503, 982, 1097, 1106, 1187, 1196, 1303, 1370
HpyF3I CTNAG 7 cut(s) 213, 331, 741, 825, 1022, 1584, 1599
Hsp92II CATG 6 cut(s) 355, 688, 895, 956, 965, 1007
HspAI GCGC 1 cut(s) 422
Ksp22I TGATCA 1 cut(s) 385
Kzo9I GATC 5 cut(s) 126, 385, 667, 969, 1529
LguI GCTCTTC 1 cut(s) 858
LmnI GCTCC 1 cut(s) 1195
Lsp1109I GCAGC 9 cut(s) 31, 104, 428, 431, 530, 766, 838, 1093, 1530
LweI GCATC 3 cut(s) 64, 250, 289
MaeI CTAG 5 cut(s) 273, 285, 656, 860, 1545
MaeIII GTNAC 6 cut(s) 428, 701, 764, 941, 1423, 1548
MalI GATC 5 cut(s) 128, 387, 669, 971, 1531
MboI GATC 5 cut(s) 126, 385, 667, 969, 1529
MflI RGATCY 1 cut(s) 667
MhlI GDGCHC 4 cut(s) 742, 927, 1544, 1590
MluCI AATT 7 cut(s) 106, 136, 445, 774, 846, 1110, 1626
MmeI TCCRAC 3 cut(s) 135, 1372, 1399
Mph1103I ATGCAT 1 cut(s) 304
MroXI GAANNNNTTC 1 cut(s) 810
MseI TTAA 4 cut(s) 624, 792, 1047, 1605
MslI CAYNNNNRTG 4 cut(s) 186, 537, 1038, 1073
MspA1I CMGCKG 1 cut(s) 757
MspI CCGG 1 cut(s) 727
MspR9I CCNGG 3 cut(s) 727, 1125, 1173
Mva1269I GAATGC 1 cut(s) 873
MvaI CCWGG 2 cut(s) 1125, 1173
MwoI GCNNNNNNNGC 9 cut(s) 269, 503, 982, 1097, 1106, 1187, 1196, 1303, 1370
NciI CCSGG 1 cut(s) 727
NdeI CATATG 1 cut(s) 615
NdeII GATC 5 cut(s) 126, 385, 667, 969, 1529
NheI GCTAGC 1 cut(s) 655
NlaIII CATG 6 cut(s) 355, 688, 895, 956, 965, 1007
NlaIV GGNNCC 1 cut(s) 669
NmeAIII GCCGAG 1 cut(s) 1225
NmuCI GTSAC 3 cut(s) 428, 941, 1548
NsiI ATGCAT 1 cut(s) 304
OliI CACNNNNGTG 2 cut(s) 186, 1073
PciSI GCTCTTC 1 cut(s) 858
PcsI WCGNNNNNNNCGW 1 cut(s) 988
PctI GAATGC 1 cut(s) 873
PdmI GAANNNNTTC 1 cut(s) 810
PfeI GAWTC 3 cut(s) 178, 1226, 1580
PfoI TCCNGGA 1 cut(s) 1171
Psp124BI GAGCTC 3 cut(s) 742, 927, 1590
Psp6I CCWGG 2 cut(s) 1123, 1171
PspGI CCWGG 2 cut(s) 1123, 1171
PspN4I GGNNCC 1 cut(s) 669
PsrI GAACNNNNNNTAC 2 cut(s) 1248, 1280
PstI CTGCAG 2 cut(s) 418, 520
PstNI CAGNNNCTG 2 cut(s) 757, 1583
PsuI RGATCY 1 cut(s) 667
PvuII CAGCTG 1 cut(s) 757
RsaI GTAC 3 cut(s) 254, 348, 1438
RsaNI GTAC 3 cut(s) 253, 347, 1437
RseI CAYNNNNRTG 4 cut(s) 186, 537, 1038, 1073
SacI GAGCTC 3 cut(s) 742, 927, 1590
SapI GCTCTTC 1 cut(s) 858
SaqAI TTAA 4 cut(s) 624, 792, 1047, 1605
Sau3AI GATC 5 cut(s) 126, 385, 667, 969, 1529
ScaI AGTACT 1 cut(s) 254
ScrFI CCNGG 3 cut(s) 727, 1125, 1173
SduI GDGCHC 4 cut(s) 742, 927, 1544, 1590
SfaNI GCATC 3 cut(s) 64, 250, 289
SfcI CTRYAG 3 cut(s) 414, 516, 1254
SmiMI CAYNNNNRTG 4 cut(s) 186, 537, 1038, 1073
SmlI CTYRAG 2 cut(s) 556, 1496
SmoI CTYRAG 2 cut(s) 556, 1496
Sse9I AATT 7 cut(s) 106, 136, 445, 774, 846, 1110, 1626
SsiI CCGC 4 cut(s) 67, 209, 469, 665
SspMI CTAG 5 cut(s) 273, 285, 656, 860, 1545
SstI GAGCTC 3 cut(s) 742, 927, 1590
StyD4I CCNGG 3 cut(s) 725, 1123, 1171
TaaI ACNGT 8 cut(s) 257, 382, 811, 937, 1096, 1120, 1377, 1441
TaqI TCGA 2 cut(s) 374, 1525
TasI AATT 7 cut(s) 106, 136, 445, 774, 846, 1110, 1626
TatI WGTACW 1 cut(s) 252
TauI GCSGC 2 cut(s) 212, 471
TfiI GAWTC 3 cut(s) 178, 1226, 1580
Tru1I TTAA 4 cut(s) 624, 792, 1047, 1605
Tru9I TTAA 4 cut(s) 624, 792, 1047, 1605
TscAI CASTG 9 cut(s) 411, 418, 712, 826, 946, 1380, 1446, 1575, 1603
TseFI GTSAC 3 cut(s) 428, 941, 1548
TseI GCWGC 9 cut(s) 19, 92, 416, 419, 518, 754, 851, 1106, 1518
Tsp45I GTSAC 3 cut(s) 428, 941, 1548
TspDTI ATGAA 5 cut(s) 174, 185, 263, 500, 1231
TspRI CASTG 9 cut(s) 411, 418, 712, 826, 946, 1380, 1446, 1575, 1603
XapI RAATTY 2 cut(s) 106, 445
XbaI TCTAGA 1 cut(s) 859
XcmI CCANNNNNNNNNTGG 1 cut(s) 1072
XmnI GAANNNNTTC 1 cut(s) 810
XspI CTAG 5 cut(s) 273, 285, 656, 860, 1545
ZrmI AGTACT 1 cut(s) 254
Zsp2I ATGCAT 1 cut(s) 304
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.