RLG00000024469

ankyrin repeat-containing protein At5g02620-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0004020
Physical Location & Seq
Reverse (-)
36123588 .. 36125645
2058 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000024469

Sequence Viewer

Length: 1638 bp
ATGGAAGTACCTGTGAGGCAGCAAAGCTTCTGCGAGAAGAAGATGACAAAGCAGTTGACAGGGAAGCGCGAAGATGCCCCCTTGCATTTGGCAGCAAGAGCAGGAAATTTGGGATTGGTGTTAGAAATCATCTCAAATGGTGGGGATGCAGAGTTGAATGAAGTGTTGATGAAGCAGAATCACTCGGGTGAAACCGCCTTGTATGTGGCGGCTGAGTGTGGCTATGTTGATTTGGTGAAGGAAATGATCAAGTACTGTGATGTTGGTTTAGCTAGCATCAAAGCTAGAAATGGCTGTGATGCATTTCACATTGCTTCCAAGCAAGGTCACTTGGAGGTATTGAAGGTTCTCATGAAGGCAATACCAGAACTTTCAATGACCGTTGATCAAACAAACACCACAGCATTGCACGCCGCAGCAGCGCAGGGTCACATTGAAGTAGTAAATTTCCTTTTGGAGAATGGTTGCAGCTTGCTGACCATAGCAAGAAGCAATGCAAAAACCGCCTTTCATTCTGCAGCAAGAAATGGTCATTTAGATGTCATCAAGGCACTCTTGAGAAAAGAACCTGAAATTGCAACGAGAAAGGATAAGAAGGGGCAGACAGCTCTCCATATGGCAGTTAAGGGACAGAATGTTGAGCTGGTGGATGAGTTAGCAAAAGCGGATCCTTCTATGATAAATATGATGGATAACAAGGGCAACACTGCCTTGCATATAGCAACCCGGAAGGGTCGAGCTCAGATTGTTCAGCAGCTGTTGGGTTATAAGGGAATTGCCACAAGTACCATTAACAACTCTGGTGAAACTGTTCTTGACACTGCACAGAAAAATGGGCAATCTGTGATTGCTGCCATTCTAGAAGAGAAAGGCATTCAAAGTGCCAAATCCATGAAACCACCAACCATAATCGCAAACCGAGAGCTCAAACAAACTGTCAGTGACATAAAACATGAGGTGCATGACCAGATCAAGCAAACACGACATACACGAAAGCAAGTACATGGTATGGTAAAACGGCTCAGCAAAATGCACTCAGATGGGCTTAACAACGCAATAAACTCCACCACTGTTGTGGCTGTCCTCATTGCCACCGTTGCCTTTGCTGCAATTTTCACTGTCCCAGGCCAATACCCCAATGACCAGGATAATCTTCCACCTGGATACTCTCCTGGAGAAGCAAACATTGCCCCAAAAGCCGGGTTCATAGTCTTCTTCATCTTTGATTCTTTTGCACTCTTCATATCATTGGCTGTAGTGGTGGTTCAGACTTCTATTGTTGTTATAGAGAGGAAGCAAAAAGAGAAACTGATGACAGTTATAAACAAGCTGATGTGGATGGCTTGTGTGATGGTTTCGGTTGCATTTCTTGCACTGTCTTATGTAATCGTCGGAGAGAAAGAGAAATGGTTGGCTGTTGGAGTAACAGGTATAGGTACAGTGATTATGGGAATGACATTGGGAACAATGTGTTATTGGATGGTTGTGCAGAGAATTGAGGCTTCAAAACTTCGTAGCATCACTCGGAGATCATCAATGAGCACTAGGTCACACTCACGTTCTGGGTCTTTCTCAGTGATGTCAGACTCTGAGCTTATCAACACTGAGTTTAAGAAGAAGAAAGTGTATGCAATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005886 GO:0012505 GO:0016020 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 Ank_3 Ank_4 Ank_5 PGG

Protein Analysis

546

Amino Acids

59.51

Weight (kDa)

9.37

Isoelectric Point (pI)

36.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 20 - 88 1.1e-07 Ankyrin repeats (3 copies)
Ank_2 PF12796 95 - 158 8.9e-12 Ankyrin repeats (3 copies)
Ank_4 PF13637 103 - 152 3.9e-07 Ankyrin repeats (many copies)
Ank_4 PF13637 136 - 186 4.4e-09 Ankyrin repeats (many copies)
Ank_2 PF12796 165 - 224 6.5e-11 Ankyrin repeats (3 copies)
Ank_4 PF13637 171 - 216 2.6e-08 Ankyrin repeats (many copies)
Ank_5 PF13857 186 - 241 8.2e-08 Ankyrin repeats (many copies)
Ank_2 PF12796 215 - 292 7.7e-09 Ankyrin repeats (3 copies)
PGG PF13962 348 - 462 1.5e-29 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 768, 1322
AccII CGCG 1 cut(s) 69
AciI CCGC 5 cut(s) 195, 209, 414, 504, 665
AclWI GGATC 2 cut(s) 662, 675
AcsI RAATTY 2 cut(s) 106, 445
AfaI GTAC 5 cut(s) 9, 254, 787, 1002, 1438
AfiI CCNNNNNNNGG 1 cut(s) 1199
AgsI TTSAA 6 cut(s) 157, 343, 375, 437, 878, 1506
AjnI CCWGG 4 cut(s) 1123, 1143, 1159, 1171
AleI CACNNNNGTG 2 cut(s) 186, 1073
Alw21I GWGCWC 3 cut(s) 742, 927, 1544
AlwI GGATC 2 cut(s) 662, 675
AlwNI CAGNNNCTG 2 cut(s) 757, 1589
Ama87I CYCGRG 1 cut(s) 184
AoxI GGCC 1 cut(s) 1126
ApeKI GCWGC 9 cut(s) 19, 92, 416, 419, 468, 518, 754, 851, 1106
ApoI RAATTY 2 cut(s) 106, 445
ArsI GACNNNNNNTTYG 2 cut(s) 921, 953
Asp700I GAANNNNTTC 1 cut(s) 810
AspLEI GCGC 2 cut(s) 69, 424
AsuC2I CCSGG 2 cut(s) 727, 1201
AsuHPI GGTGA 3 cut(s) 200, 247, 815
AsuNHI GCTAGC 1 cut(s) 272
AvaI CYCGRG 1 cut(s) 184
BamHI GGATCC 1 cut(s) 667
BanII GRGCYC 2 cut(s) 742, 927
BbsI GAAGAC 1 cut(s) 1204
Bbv12I GWGCWC 3 cut(s) 742, 927, 1544
BbvI GCAGC 9 cut(s) 31, 104, 428, 431, 480, 530, 766, 838, 1093
BccI CCATC 5 cut(s) 682, 1034, 1333, 1345, 1474
BceAI ACGGC 1 cut(s) 1034
BciT130I CCWGG 4 cut(s) 1125, 1145, 1161, 1173
BciVI GTATCC 1 cut(s) 1157
BclI TGATCA 2 cut(s) 246, 385
BcnI CCSGG 2 cut(s) 727, 1201
BfaI CTAG 4 cut(s) 273, 285, 860, 1545
BfmI CTRYAG 2 cut(s) 516, 1254
BfuI GTATCC 1 cut(s) 1157
BlpI GCTNAGC 1 cut(s) 1022
BmcAI AGTACT 1 cut(s) 254
Bme1390I CCNGG 6 cut(s) 727, 1125, 1145, 1161, 1173, 1201
BmeT110I CYCGRG 1 cut(s) 184
BmiI GGNNCC 1 cut(s) 669
BmrFI CCNGG 6 cut(s) 727, 1125, 1145, 1161, 1173, 1201
BmsI GCATC 5 cut(s) 64, 136, 285, 289, 1527
BmtI GCTAGC 1 cut(s) 276
BpiI GAAGAC 1 cut(s) 1204
BpmI CTGGAG 1 cut(s) 1194
Bpu1102I GCTNAGC 1 cut(s) 1022
BpuEI CTTGAG 1 cut(s) 577
BpuMI CCSGG 2 cut(s) 727, 1201
BsaJI CCNNGG 1 cut(s) 1123
Bsc4I CCNNNNNNNGG 1 cut(s) 1199
Bse3DI GCAATG 5 cut(s) 309, 404, 499, 1086, 1185
BseBI CCWGG 4 cut(s) 1125, 1145, 1161, 1173
BseDI CCNNGG 1 cut(s) 1123
BseGI GGATG 4 cut(s) 151, 655, 1344, 1485
BseLI CCNNNNNNNGG 1 cut(s) 1199
BseMI GCAATG 5 cut(s) 309, 404, 499, 1086, 1185
BseMII CTCAG 7 cut(s) 204, 755, 1036, 1050, 1581, 1587, 1596
BseXI GCAGC 9 cut(s) 31, 104, 428, 431, 480, 530, 766, 838, 1093
BsgI GTGCAG 2 cut(s) 807, 1508
Bsh1236I CGCG 1 cut(s) 69
BshFI GGCC 1 cut(s) 1128
BsiHKAI GWGCWC 3 cut(s) 742, 927, 1544
BsiHKCI CYCGRG 1 cut(s) 184
BsiSI CCGG 2 cut(s) 727, 1200
BslFI GGGAC 2 cut(s) 642, 1106
BslI CCNNNNNNNGG 1 cut(s) 1199
BsmFI GGGAC 2 cut(s) 642, 1106
BsmI GAATGC 1 cut(s) 873
BsnI GGCC 1 cut(s) 1128
BsoBI CYCGRG 1 cut(s) 184
Bsp1286I GDGCHC 3 cut(s) 742, 927, 1544
Bsp143I GATC 5 cut(s) 246, 385, 667, 969, 1529
Bsp1720I GCTNAGC 1 cut(s) 1022
BspACI CCGC 5 cut(s) 195, 209, 414, 504, 665
BspANI GGCC 1 cut(s) 1128
BspCNI CTCAG 7 cut(s) 205, 754, 1035, 1049, 1582, 1586, 1597
BspFNI CGCG 1 cut(s) 69
BspHI TCATGA 1 cut(s) 351
BspLI GGNNCC 1 cut(s) 669
BspMAI CTGCAG 1 cut(s) 520
BspOI GCTAGC 1 cut(s) 276
BspPI GGATC 2 cut(s) 662, 675
BsrDI GCAATG 5 cut(s) 309, 404, 499, 1086, 1185
BssECI CCNNGG 1 cut(s) 1123
BssMI GATC 5 cut(s) 246, 385, 667, 969, 1529
Bst2UI CCWGG 4 cut(s) 1125, 1145, 1161, 1173
Bst6I CTCTTC 2 cut(s) 858, 1244
BstAPI GCANNNNNTGC 2 cut(s) 1187, 1370
BstC8I GCNNGC 3 cut(s) 274, 411, 473
BstDEI CTNAG 7 cut(s) 213, 741, 1022, 1036, 1573, 1590, 1605
BstF5I GGATG 4 cut(s) 151, 655, 1344, 1485
BstFNI CGCG 1 cut(s) 69
BstHHI GCGC 2 cut(s) 69, 424
BstKTI GATC 5 cut(s) 249, 388, 670, 972, 1532
BstMBI GATC 5 cut(s) 246, 385, 667, 969, 1529
BstMWI GCNNNNNNNGC 9 cut(s) 98, 410, 419, 503, 1097, 1106, 1187, 1196, 1370
BstNI CCWGG 4 cut(s) 1125, 1145, 1161, 1173
BstSCI CCNGG 6 cut(s) 725, 1123, 1143, 1159, 1171, 1199
BstSFI CTRYAG 2 cut(s) 516, 1254
BstUI CGCG 1 cut(s) 69
BstV1I GCAGC 9 cut(s) 31, 104, 428, 431, 480, 530, 766, 838, 1093
BstV2I GAAGAC 1 cut(s) 1204
BstX2I RGATCY 1 cut(s) 667
BstXI CCANNNNNNTGG 1 cut(s) 1075
BstYI RGATCY 1 cut(s) 667
BsuI GTATCC 1 cut(s) 1157
BsuRI GGCC 1 cut(s) 1128
BtsCI GGATG 4 cut(s) 151, 655, 1344, 1485
BtsI GCAGTG 2 cut(s) 705, 819
BtsIMutI CAGTG 9 cut(s) 705, 819, 946, 1068, 1116, 1373, 1446, 1581, 1602
Cac8I GCNNGC 3 cut(s) 274, 411, 473
CaiI CAGNNNCTG 2 cut(s) 757, 1589
CciI TCATGA 1 cut(s) 351
CfoI GCGC 2 cut(s) 69, 424
Csp6I GTAC 5 cut(s) 8, 253, 786, 1001, 1437
CspCI CAANNNNNGTGG 2 cut(s) 1054, 1089
CviAII CATG 5 cut(s) 352, 892, 953, 962, 1004
CviQI GTAC 5 cut(s) 8, 253, 786, 1001, 1437
DdeI CTNAG 7 cut(s) 213, 741, 1022, 1036, 1573, 1590, 1605
DpnI GATC 5 cut(s) 248, 387, 669, 971, 1531
DpnII GATC 5 cut(s) 246, 385, 667, 969, 1529
Eam1104I CTCTTC 2 cut(s) 858, 1244
EarI CTCTTC 2 cut(s) 858, 1244
Ecl136II GAGCTC 2 cut(s) 740, 925
Eco24I GRGCYC 2 cut(s) 742, 927
Eco53kI GAGCTC 2 cut(s) 740, 925
Eco88I CYCGRG 1 cut(s) 184
EcoICRI GAGCTC 2 cut(s) 740, 925
EcoRII CCWGG 4 cut(s) 1123, 1143, 1159, 1171
EcoT22I ATGCAT 1 cut(s) 304
EcoT38I GRGCYC 2 cut(s) 742, 927
FaeI CATG 5 cut(s) 355, 895, 956, 965, 1007
FalI AAGNNNNNCTT 2 cut(s) 539, 571
FaqI GGGAC 2 cut(s) 642, 1106
FatI CATG 5 cut(s) 351, 891, 952, 961, 1003
FauNDI CATATG 1 cut(s) 615
FbaI TGATCA 2 cut(s) 246, 385
FokI GGATG 4 cut(s) 158, 662, 1351, 1492
FriOI GRGCYC 2 cut(s) 742, 927
FspBI CTAG 4 cut(s) 273, 285, 860, 1545
GlaI GCGC 2 cut(s) 68, 423
GsuI CTGGAG 1 cut(s) 1194
HaeIII GGCC 1 cut(s) 1128
HapII CCGG 2 cut(s) 727, 1200
HhaI GCGC 2 cut(s) 69, 424
Hin1II CATG 5 cut(s) 355, 895, 956, 965, 1007
Hin6I GCGC 2 cut(s) 67, 422
HinP1I GCGC 2 cut(s) 67, 422
HincII GTYRAC 1 cut(s) 57
HindII GTYRAC 1 cut(s) 57
HindIII AAGCTT 1 cut(s) 25
HinfI GANTC 3 cut(s) 178, 1226, 1586
HpaII CCGG 2 cut(s) 727, 1200
HphI GGTGA 3 cut(s) 200, 247, 815
Hpy166II GTNNAC 1 cut(s) 57
Hpy188I TCNGA 7 cut(s) 744, 1039, 1269, 1394, 1527, 1585, 1591
Hpy188III TCNNGA 4 cut(s) 352, 556, 815, 860
Hpy8I GTNNAC 1 cut(s) 57
Hpy99I CGWCG 1 cut(s) 1394
HpyAV CCTTC 6 cut(s) 232, 337, 349, 589, 681, 724
HpyCH4IV ACGT 1 cut(s) 1558
HpyF10VI GCNNNNNNNGC 9 cut(s) 98, 410, 419, 503, 1097, 1106, 1187, 1196, 1370
HpyF3I CTNAG 7 cut(s) 213, 741, 1022, 1036, 1573, 1590, 1605
HpySE526I ACGT 1 cut(s) 1558
Hsp92II CATG 5 cut(s) 355, 895, 956, 965, 1007
HspAI GCGC 2 cut(s) 67, 422
Ksp22I TGATCA 2 cut(s) 246, 385
Kzo9I GATC 5 cut(s) 246, 385, 667, 969, 1529
Lsp1109I GCAGC 9 cut(s) 31, 104, 428, 431, 480, 530, 766, 838, 1093
LweI GCATC 5 cut(s) 64, 136, 285, 289, 1527
MaeI CTAG 4 cut(s) 273, 285, 860, 1545
MaeII ACGT 1 cut(s) 1558
MaeIII GTNAC 5 cut(s) 326, 428, 941, 1423, 1548
MalI GATC 5 cut(s) 248, 387, 669, 971, 1531
MboI GATC 5 cut(s) 246, 385, 667, 969, 1529
MflI RGATCY 1 cut(s) 667
MhlI GDGCHC 3 cut(s) 742, 927, 1544
MluCI AATT 7 cut(s) 106, 445, 573, 774, 1110, 1494, 1632
MlyI GAGTC 1 cut(s) 1580
MmeI TCCRAC 2 cut(s) 1372, 1399
MnlI CCTC 6 cut(s) 9, 328, 949, 1094, 1284, 1492
Mph1103I ATGCAT 1 cut(s) 304
MroXI GAANNNNTTC 1 cut(s) 810
MseI TTAA 4 cut(s) 624, 792, 1047, 1611
MslI CAYNNNNRTG 4 cut(s) 186, 537, 1038, 1073
MspA1I CMGCKG 1 cut(s) 757
MspI CCGG 2 cut(s) 727, 1200
MspR9I CCNGG 6 cut(s) 727, 1125, 1145, 1161, 1173, 1201
Mva1269I GAATGC 1 cut(s) 873
MvaI CCWGG 4 cut(s) 1125, 1145, 1161, 1173
MvnI CGCG 1 cut(s) 69
MwoI GCNNNNNNNGC 9 cut(s) 98, 410, 419, 503, 1097, 1106, 1187, 1196, 1370
NciI CCSGG 2 cut(s) 727, 1201
NdeI CATATG 1 cut(s) 615
NdeII GATC 5 cut(s) 246, 385, 667, 969, 1529
NheI GCTAGC 1 cut(s) 272
NlaIII CATG 5 cut(s) 355, 895, 956, 965, 1007
NlaIV GGNNCC 1 cut(s) 669
NmuCI GTSAC 4 cut(s) 326, 428, 941, 1548
NsiI ATGCAT 1 cut(s) 304
OliI CACNNNNGTG 2 cut(s) 186, 1073
PagI TCATGA 1 cut(s) 351
PcsI WCGNNNNNNNCGW 1 cut(s) 988
PctI GAATGC 1 cut(s) 873
PdmI GAANNNNTTC 1 cut(s) 810
PfeI GAWTC 2 cut(s) 178, 1226
PfoI TCCNGGA 1 cut(s) 1171
PleI GAGTC 1 cut(s) 1580
PpsI GAGTC 1 cut(s) 1580
PsiI TTATAA 2 cut(s) 768, 1322
Psp124BI GAGCTC 2 cut(s) 742, 927
Psp6I CCWGG 4 cut(s) 1123, 1143, 1159, 1171
PspGI CCWGG 4 cut(s) 1123, 1143, 1159, 1171
PspN4I GGNNCC 1 cut(s) 669
PsrI GAACNNNNNNTAC 4 cut(s) 330, 362, 1248, 1280
PstI CTGCAG 1 cut(s) 520
PstNI CAGNNNCTG 2 cut(s) 757, 1589
PsuI RGATCY 1 cut(s) 667
PvuII CAGCTG 1 cut(s) 757
RsaI GTAC 5 cut(s) 9, 254, 787, 1002, 1438
RsaNI GTAC 5 cut(s) 8, 253, 786, 1001, 1437
RseI CAYNNNNRTG 4 cut(s) 186, 537, 1038, 1073
SacI GAGCTC 2 cut(s) 742, 927
SaqAI TTAA 4 cut(s) 624, 792, 1047, 1611
Sau3AI GATC 5 cut(s) 246, 385, 667, 969, 1529
ScaI AGTACT 1 cut(s) 254
SchI GAGTC 1 cut(s) 1580
ScrFI CCNGG 6 cut(s) 727, 1125, 1145, 1161, 1173, 1201
SduI GDGCHC 3 cut(s) 742, 927, 1544
SfaNI GCATC 5 cut(s) 64, 136, 285, 289, 1527
SfcI CTRYAG 2 cut(s) 516, 1254
SmiMI CAYNNNNRTG 4 cut(s) 186, 537, 1038, 1073
SmlI CTYRAG 1 cut(s) 556
SmoI CTYRAG 1 cut(s) 556
Sse9I AATT 7 cut(s) 106, 445, 573, 774, 1110, 1494, 1632
SsiI CCGC 5 cut(s) 195, 209, 414, 504, 665
SspMI CTAG 4 cut(s) 273, 285, 860, 1545
SstI GAGCTC 2 cut(s) 742, 927
StyD4I CCNGG 6 cut(s) 725, 1123, 1143, 1159, 1171, 1199
TaiI ACGT 1 cut(s) 1561
TaqI TCGA 1 cut(s) 736
TasI AATT 7 cut(s) 106, 445, 573, 774, 1110, 1494, 1632
TatI WGTACW 2 cut(s) 252, 1000
TauI GCSGC 2 cut(s) 212, 416
TfiI GAWTC 2 cut(s) 178, 1226
Tru1I TTAA 4 cut(s) 624, 792, 1047, 1611
Tru9I TTAA 4 cut(s) 624, 792, 1047, 1611
TscAI CASTG 9 cut(s) 712, 826, 946, 1075, 1123, 1380, 1446, 1581, 1609
TseFI GTSAC 4 cut(s) 326, 428, 941, 1548
TseI GCWGC 9 cut(s) 19, 92, 416, 419, 468, 518, 754, 851, 1106
Tsp45I GTSAC 4 cut(s) 326, 428, 941, 1548
TspDTI ATGAA 8 cut(s) 174, 185, 368, 500, 908, 1195, 1207, 1231
TspRI CASTG 9 cut(s) 712, 826, 946, 1075, 1123, 1380, 1446, 1581, 1609
XapI RAATTY 2 cut(s) 106, 445
XbaI TCTAGA 1 cut(s) 859
XcmI CCANNNNNNNNNTGG 1 cut(s) 1072
XmnI GAANNNNTTC 1 cut(s) 810
XspI CTAG 4 cut(s) 273, 285, 860, 1545
ZrmI AGTACT 1 cut(s) 254
Zsp2I ATGCAT 1 cut(s) 304
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.