MD12G1132900.v1.1

ankyrin repeat-containing protein At5g02620-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0004020
Physical Location & Seq
Reverse (-)
20845998 .. 20848984
2987 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1132900.v1.1.491

Sequence Viewer

Length: 1656 bp
ATGGAAGCTCCAGTGAGGCAGCAAAGCTTTGTTGGGAAGAAGATGACTAAGCAGTTGACGGGAAAGCGCGAAGACGGGCCGTTGCATTTGGCAGCAAGAGAAGGAGATTTGGGATTGGTGGTACAAATCTTGTCCAAGTGTGGGGAGGCAGAGCTGAATGAACTTTTGTCGAAACAGAACCACATGGGGGAAACCCCCTTGTATCTGGCGGCTGATTGTGGATATGTTGATTTGGTTAAGGAAATGATGAAGTACTATGATGTTGGTTCAGCTGGTATCAAAGCTCGAAATGGCTGTGATGCATTTCACATTGCTTCCAAGCAAGGGCACTTAGAGGTACTGAAGGTCCTCATGGAGGCGATTCCAGAACTTTCAATGACCGTTGATCGCACAAACACCACGGCATTGCACATCGCTGCAGCACAGGGACACACAGAAGTAGTAAGTTTTCTTTTGGAGACTGGTTGCAGCTTGGTGTCCATAGCAAGAAGCAATGGGAAAACTGCCTTGCATTCTGCAGCAAGGAAAGGGCATTTGGAGGTGATCCAGGCCCTCTTGAGCAAAGATACCGGAATTGCATTAAGAAGAGATAATAAGGGCCAGACAGCTCTCCATATGGCAGTCAAGGGACAGAATGTCGAATTGGTGGGTGAGCTAGCGAAGGCAGATCCTTCGGTGATAAACGTGGTGGATAACAAGGGAAACACTGCCTTGCATGTGGCAACAGGGAAGGGTCACGCTCAGATTGTTCAAACGCTGTTAAGTCATAAGGGAGTGGCGAAAACCATCATTAACAGATCTGGAGAATCTGTTTTCGACACTGCTGAGAAATCTGGGCACACAAAGATTGCTACCATTCTAGCAGAGCATGGGATTCAAGGTGCCAAATCCATGAAGCTGCCAACTATGAACCCAAATCGAGAACTCAAACAAACTGTAAGCGAAATAAAACATGAGGTGCATGATCAGCTTAAGCAAACGAGACAAACACGAAAGCAAGTGCATGGAATGGTAAAGCGGCTCAGCAAAATGCATTTGGAGGGGCTTAACAATGCAATAAACTCCACCACAGTTGTGGCTGTCCTCATTGCCACTGTCGCCTTTGCTGCAATCTTCACTGTCCCAGGCCAATACCCTGACCCTGAGAAACCTCTTCCCCGTGGATTCTCTCCCGGAGAAGCAAACATTGCCCCCAAACCTGAGTTCCTAGTCTTCTTCATCTTTGACTCTTTTGCTCTCTTCATATCGTTGGCTGTCGTGGTGGTTCAAACTTCTATTGTTGTCGTAGAGAGGGAGGCAAAAGAGAAGATGATGTCAATCATAAACAAGCTAATGTGGATGGCTTGTGTGATGATTTCAGTTTCATTTCTTGCACTGTCATATATAATTGTTGGAAAAGAAGAGAAATGGCTGGCTATCGGAGTGACAGCTATAGGGACAGTGATCATGGGAATGACATTAGGAACAATGTGTTATTGGATGGTTGTGCAGCGCGTTGAAGCTTCTAAACATCGTCGCAGCCTTCGGAGATCATCAATGAGCACAAACTCACACTCACTCTCGTGCTCGCACTCTCACTCACCCTCTTTCTCAGTGATTTCGGATTCAGAGCTTTTGAACACCGAGTATAAGAGTAAGAAAGTTTATGCAATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005886 GO:0012505 GO:0016020 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 Ank_3 Ank_4 Ank_5 PGG

Protein Analysis

552

Amino Acids

60.05

Weight (kDa)

9.24

Isoelectric Point (pI)

33.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 23 - 87 1.2e-06 Ankyrin repeats (3 copies)
Ank_2 PF12796 93 - 158 2.1e-11 Ankyrin repeats (3 copies)
Ank_4 PF13637 103 - 152 6e-09 Ankyrin repeats (many copies)
Ank_2 PF12796 164 - 224 1.2e-11 Ankyrin repeats (3 copies)
Ank_4 PF13637 170 - 216 4.8e-08 Ankyrin repeats (many copies)
Ank_5 PF13857 195 - 241 4.1e-06 Ankyrin repeats (many copies)
Ank_2 PF12796 214 - 293 1.7e-09 Ankyrin repeats (3 copies)
PGG PF13962 347 - 462 6.4e-29 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 881
AccII CGCG 2 cut(s) 69, 1494
AciI CCGC 2 cut(s) 209, 1018
AclWI GGATC 2 cut(s) 538, 662
AcuI CTGAAG 1 cut(s) 362
AfaI GTAC 3 cut(s) 123, 254, 339
AfiI CCNNNNNNNGG 4 cut(s) 141, 187, 324, 355
AflII CTTAAG 1 cut(s) 971
AgsI TTSAA 6 cut(s) 375, 752, 878, 1268, 1499, 1618
AhdI GACNNNNNGTC 1 cut(s) 635
AjnI CCWGG 2 cut(s) 546, 1123
AjuI GAANNNNNNNTTGG 6 cut(s) 518, 550, 626, 658, 1187, 1219
AleI CACNNNNGTG 2 cut(s) 1073, 1561
Alw21I GWGCWC 2 cut(s) 1544, 1568
Alw26I GTCTC 2 cut(s) 452, 976
AlwI GGATC 2 cut(s) 538, 662
AoxI GGCC 4 cut(s) 77, 549, 598, 1126
AspLEI GCGC 2 cut(s) 69, 1494
AspS9I GGNCC 4 cut(s) 77, 346, 550, 598
AsuC2I CCSGG 1 cut(s) 1173
AsuHPI GGTGA 4 cut(s) 553, 662, 688, 1572
AsuNHI GCTAGC 1 cut(s) 655
AvaII GGWCC 1 cut(s) 346
BaeGI GKGCMC 2 cut(s) 330, 840
BanI GGYRCC 1 cut(s) 881
BauI CACGAG 1 cut(s) 1561
BbsI GAAGAC 2 cut(s) 78, 1204
Bbv12I GWGCWC 2 cut(s) 1544, 1568
BccI CCATC 3 cut(s) 794, 1333, 1474
BceAI ACGGC 2 cut(s) 64, 417
BcgI CGANNNNNNTGC 2 cut(s) 654, 688
BciT130I CCWGG 2 cut(s) 548, 1125
BclI TGATCA 2 cut(s) 964, 1443
BcnI CCSGG 1 cut(s) 1173
BcoDI GTCTC 2 cut(s) 452, 976
BfaI CTAG 3 cut(s) 656, 860, 1208
BfmI CTRYAG 3 cut(s) 417, 516, 1431
BfrI CTTAAG 1 cut(s) 971
BglII AGATCT 1 cut(s) 797
BlpI GCTNAGC 1 cut(s) 1022
BmcAI AGTACT 1 cut(s) 254
Bme1390I CCNGG 3 cut(s) 548, 1125, 1173
Bme18I GGWCC 1 cut(s) 346
BmeRI GACNNNNNGTC 1 cut(s) 635
BmgT120I GGNCC 4 cut(s) 77, 346, 550, 598
BmiI GGNNCC 1 cut(s) 883
BmrFI CCNGG 3 cut(s) 548, 1125, 1173
BmsI GCATC 1 cut(s) 289
BmtI GCTAGC 1 cut(s) 659
BpiI GAAGAC 2 cut(s) 78, 1204
BpmI CTGGAG 1 cut(s) 822
Bpu1102I GCTNAGC 1 cut(s) 1022
BpuEI CTTGAG 1 cut(s) 577
BpuMI CCSGG 1 cut(s) 1173
BsaBI GATNNNNATC 1 cut(s) 1316
BsaJI CCNNGG 3 cut(s) 399, 1123, 1159
BsaWI WCCGGW 1 cut(s) 569
BsaXI ACNNNNNCTCC 2 cut(s) 795, 825
Bsc4I CCNNNNNNNGG 4 cut(s) 141, 187, 324, 355
Bse1I ACTGG 2 cut(s) 11, 466
Bse3DI GCAATG 5 cut(s) 309, 404, 499, 1086, 1185
Bse8I GATNNNNATC 1 cut(s) 1316
BseBI CCWGG 2 cut(s) 548, 1125
BseDI CCNNGG 3 cut(s) 399, 1123, 1159
BseGI GGATG 2 cut(s) 1344, 1485
BseJI GATNNNNATC 1 cut(s) 1316
BseLI CCNNNNNNNGG 4 cut(s) 141, 187, 324, 355
BseMI GCAATG 5 cut(s) 309, 404, 499, 1086, 1185
BseMII CTCAG 6 cut(s) 755, 816, 1036, 1134, 1191, 1605
BseNI ACTGG 2 cut(s) 11, 466
BseSI GKGCMC 2 cut(s) 330, 840
BsgI GTGCAG 1 cut(s) 1508
Bsh1236I CGCG 2 cut(s) 69, 1494
BshFI GGCC 4 cut(s) 79, 551, 600, 1128
BshNI GGYRCC 1 cut(s) 881
BsiHKAI GWGCWC 2 cut(s) 1544, 1568
BsiSI CCGG 2 cut(s) 570, 1173
BslFI GGGAC 4 cut(s) 441, 642, 1106, 1450
BslI CCNNNNNNNGG 4 cut(s) 141, 187, 324, 355
BsmAI GTCTC 2 cut(s) 452, 976
BsmFI GGGAC 4 cut(s) 441, 642, 1106, 1450
BsmI GAATGC 1 cut(s) 511
BsnI GGCC 4 cut(s) 79, 551, 600, 1128
Bsp1286I GDGCHC 4 cut(s) 330, 840, 1544, 1568
Bsp143I GATC 7 cut(s) 385, 543, 667, 797, 964, 1443, 1529
Bsp1720I GCTNAGC 1 cut(s) 1022
BspACI CCGC 2 cut(s) 209, 1018
BspANI GGCC 4 cut(s) 79, 551, 600, 1128
BspCNI CTCAG 6 cut(s) 754, 817, 1035, 1135, 1192, 1604
BspFNI CGCG 2 cut(s) 69, 1494
BspLI GGNNCC 1 cut(s) 883
BspMAI CTGCAG 2 cut(s) 421, 520
BspOI GCTAGC 1 cut(s) 659
BspPI GGATC 2 cut(s) 538, 662
BspT107I GGYRCC 1 cut(s) 881
BspTI CTTAAG 1 cut(s) 971
BsrDI GCAATG 5 cut(s) 309, 404, 499, 1086, 1185
BsrI ACTGG 2 cut(s) 11, 466
BssECI CCNNGG 3 cut(s) 399, 1123, 1159
BssMI GATC 7 cut(s) 385, 543, 667, 797, 964, 1443, 1529
BssSI CACGAG 1 cut(s) 1561
Bst2BI CACGAG 1 cut(s) 1561
Bst2UI CCWGG 2 cut(s) 548, 1125
Bst4CI ACNGT 7 cut(s) 382, 937, 1072, 1096, 1120, 1377, 1441
Bst6I CTCTTC 4 cut(s) 580, 1158, 1244, 1395
BstAFI CTTAAG 1 cut(s) 971
BstAPI GCANNNNNTGC 1 cut(s) 1187
BstC8I GCNNGC 3 cut(s) 657, 1413, 1568
BstDEI CTNAG 8 cut(s) 48, 331, 741, 825, 1022, 1143, 1200, 1591
BstDSI CCRYGG 2 cut(s) 399, 1159
BstENI CCTNNNNNAGG 1 cut(s) 353
BstF5I GGATG 2 cut(s) 1344, 1485
BstFNI CGCG 2 cut(s) 69, 1494
BstHHI GCGC 2 cut(s) 69, 1494
BstKTI GATC 7 cut(s) 388, 546, 670, 800, 967, 1446, 1532
BstMAI GTCTC 2 cut(s) 452, 976
BstMBI GATC 7 cut(s) 385, 543, 667, 797, 964, 1443, 1529
BstMWI GCNNNNNNNGC 4 cut(s) 967, 1097, 1106, 1187
BstNI CCWGG 2 cut(s) 548, 1125
BstNSI RCATGY 1 cut(s) 719
BstSCI CCNGG 3 cut(s) 546, 1123, 1171
BstSFI CTRYAG 3 cut(s) 417, 516, 1431
BstSLI GKGCMC 2 cut(s) 330, 840
BstUI CGCG 2 cut(s) 69, 1494
BstV2I GAAGAC 2 cut(s) 78, 1204
BstX2I RGATCY 2 cut(s) 667, 797
BstXI CCANNNNNNTGG 1 cut(s) 1075
BstYI RGATCY 2 cut(s) 667, 797
BsuRI GGCC 4 cut(s) 79, 551, 600, 1128
BtgI CCRYGG 2 cut(s) 399, 1159
BtgZI GCGATG 1 cut(s) 397
BtsCI GGATG 2 cut(s) 1344, 1485
BtsI GCAGTG 2 cut(s) 705, 819
BtsIMutI CAGTG 8 cut(s) 18, 705, 819, 1092, 1116, 1373, 1446, 1599
Cac8I GCNNGC 3 cut(s) 657, 1413, 1568
CfoI GCGC 2 cut(s) 69, 1494
Cfr13I GGNCC 4 cut(s) 77, 346, 550, 598
Csp6I GTAC 3 cut(s) 122, 253, 338
CspCI CAANNNNNGTGG 2 cut(s) 1054, 1089
CviAII CATG 9 cut(s) 184, 352, 716, 869, 892, 953, 962, 1004, 1447
CviQI GTAC 3 cut(s) 122, 253, 338
DdeI CTNAG 8 cut(s) 48, 331, 741, 825, 1022, 1143, 1200, 1591
DpnI GATC 7 cut(s) 387, 545, 669, 799, 966, 1445, 1531
DpnII GATC 7 cut(s) 385, 543, 667, 797, 964, 1443, 1529
DriI GACNNNNNGTC 1 cut(s) 635
Eam1104I CTCTTC 4 cut(s) 580, 1158, 1244, 1395
Eam1105I GACNNNNNGTC 1 cut(s) 635
EarI CTCTTC 4 cut(s) 580, 1158, 1244, 1395
Eco47I GGWCC 1 cut(s) 346
Eco57I CTGAAG 1 cut(s) 362
EcoNI CCTNNNNNAGG 1 cut(s) 353
EcoO109I RGGNCCY 2 cut(s) 346, 550
EcoRII CCWGG 2 cut(s) 546, 1123
EcoT22I ATGCAT 2 cut(s) 304, 1035
FaeI CATG 9 cut(s) 187, 355, 719, 872, 895, 956, 965, 1007, 1450
FaqI GGGAC 4 cut(s) 441, 642, 1106, 1450
FatI CATG 9 cut(s) 183, 351, 715, 868, 891, 952, 961, 1003, 1446
FauNDI CATATG 1 cut(s) 615
FbaI TGATCA 2 cut(s) 964, 1443
FokI GGATG 2 cut(s) 1351, 1492
FspBI CTAG 3 cut(s) 656, 860, 1208
GlaI GCGC 2 cut(s) 68, 1493
GsuI CTGGAG 1 cut(s) 822
HaeIII GGCC 4 cut(s) 79, 551, 600, 1128
HapII CCGG 2 cut(s) 570, 1173
HhaI GCGC 2 cut(s) 69, 1494
Hin1II CATG 9 cut(s) 187, 355, 719, 872, 895, 956, 965, 1007, 1450
Hin6I GCGC 2 cut(s) 67, 1492
HinP1I GCGC 2 cut(s) 67, 1492
HincII GTYRAC 1 cut(s) 57
HindII GTYRAC 1 cut(s) 57
HindIII AAGCTT 2 cut(s) 25, 1500
HinfI GANTC 6 cut(s) 361, 806, 874, 1164, 1226, 1604
HpaII CCGG 2 cut(s) 570, 1173
HphI GGTGA 4 cut(s) 553, 662, 688, 1572
Hpy166II GTNNAC 1 cut(s) 57
Hpy188I TCNGA 5 cut(s) 744, 1421, 1527, 1603, 1609
Hpy188III TCNNGA 4 cut(s) 365, 556, 801, 920
Hpy8I GTNNAC 1 cut(s) 57
Hpy99I CGWCG 1 cut(s) 1518
HpyAV CCTTC 6 cut(s) 95, 337, 655, 681, 724, 1532
HpyCH4III ACNGT 7 cut(s) 382, 937, 1072, 1096, 1120, 1377, 1441
HpyCH4IV ACGT 1 cut(s) 684
HpyF10VI GCNNNNNNNGC 4 cut(s) 967, 1097, 1106, 1187
HpyF3I CTNAG 8 cut(s) 48, 331, 741, 825, 1022, 1143, 1200, 1591
HpySE526I ACGT 1 cut(s) 684
Hsp92II CATG 9 cut(s) 187, 355, 719, 872, 895, 956, 965, 1007, 1450
HspAI GCGC 2 cut(s) 67, 1492
Ksp22I TGATCA 2 cut(s) 964, 1443
Kzo9I GATC 7 cut(s) 385, 543, 667, 797, 964, 1443, 1529
LmnI GCTCC 1 cut(s) 13
LweI GCATC 1 cut(s) 289
MaeI CTAG 3 cut(s) 656, 860, 1208
MaeII ACGT 1 cut(s) 684
MaeIII GTNAC 2 cut(s) 734, 1423
MalI GATC 7 cut(s) 387, 545, 669, 799, 966, 1445, 1531
MboI GATC 7 cut(s) 385, 543, 667, 797, 964, 1443, 1529
MflI RGATCY 2 cut(s) 667, 797
MhlI GDGCHC 4 cut(s) 330, 840, 1544, 1568
MluCI AATT 4 cut(s) 573, 641, 1386, 1650
MlyI GAGTC 1 cut(s) 1220
MmeI TCCRAC 1 cut(s) 1372
Mph1103I ATGCAT 2 cut(s) 304, 1035
MseI TTAA 6 cut(s) 237, 581, 761, 792, 972, 1047
MslI CAYNNNNRTG 3 cut(s) 1073, 1451, 1561
MspA1I CMGCKG 1 cut(s) 272
MspCI CTTAAG 1 cut(s) 971
MspI CCGG 2 cut(s) 570, 1173
MspR9I CCNGG 3 cut(s) 548, 1125, 1173
Mva1269I GAATGC 1 cut(s) 511
MvaI CCWGG 2 cut(s) 548, 1125
MvnI CGCG 2 cut(s) 69, 1494
MwoI GCNNNNNNNGC 4 cut(s) 967, 1097, 1106, 1187
NciI CCSGG 1 cut(s) 1173
NdeI CATATG 1 cut(s) 615
NdeII GATC 7 cut(s) 385, 543, 667, 797, 964, 1443, 1529
NheI GCTAGC 1 cut(s) 655
NlaIII CATG 9 cut(s) 187, 355, 719, 872, 895, 956, 965, 1007, 1450
NlaIV GGNNCC 1 cut(s) 883
NmuCI GTSAC 2 cut(s) 734, 1423
NsiI ATGCAT 2 cut(s) 304, 1035
NspI RCATGY 1 cut(s) 719
OliI CACNNNNGTG 2 cut(s) 1073, 1561
PctI GAATGC 1 cut(s) 511
PfeI GAWTC 5 cut(s) 361, 806, 874, 1164, 1604
PfoI TCCNGGA 1 cut(s) 1171
PleI GAGTC 1 cut(s) 1220
PpsI GAGTC 1 cut(s) 1220
PpuMI RGGWCCY 1 cut(s) 346
Psp5II RGGWCCY 1 cut(s) 346
Psp6I CCWGG 2 cut(s) 546, 1123
PspGI CCWGG 2 cut(s) 546, 1123
PspN4I GGNNCC 1 cut(s) 883
PspPI GGNCC 4 cut(s) 77, 346, 550, 598
PspPPI RGGWCCY 1 cut(s) 346
PstI CTGCAG 2 cut(s) 421, 520
PsuI RGATCY 2 cut(s) 667, 797
PvuII CAGCTG 1 cut(s) 272
RsaI GTAC 3 cut(s) 123, 254, 339
RsaNI GTAC 3 cut(s) 122, 253, 338
RseI CAYNNNNRTG 3 cut(s) 1073, 1451, 1561
SaqAI TTAA 6 cut(s) 237, 581, 761, 792, 972, 1047
Sau3AI GATC 7 cut(s) 385, 543, 667, 797, 964, 1443, 1529
Sau96I GGNCC 4 cut(s) 77, 346, 550, 598
ScaI AGTACT 1 cut(s) 254
SchI GAGTC 1 cut(s) 1220
ScrFI CCNGG 3 cut(s) 548, 1125, 1173
SduI GDGCHC 4 cut(s) 330, 840, 1544, 1568
SfaNI GCATC 1 cut(s) 289
SfcI CTRYAG 3 cut(s) 417, 516, 1431
SinI GGWCC 1 cut(s) 346
SmiMI CAYNNNNRTG 3 cut(s) 1073, 1451, 1561
SmlI CTYRAG 2 cut(s) 556, 971
SmoI CTYRAG 2 cut(s) 556, 971
Sse9I AATT 4 cut(s) 573, 641, 1386, 1650
SsiI CCGC 2 cut(s) 209, 1018
SspMI CTAG 3 cut(s) 656, 860, 1208
StyD4I CCNGG 3 cut(s) 546, 1123, 1171
TaaI ACNGT 7 cut(s) 382, 937, 1072, 1096, 1120, 1377, 1441
TaiI ACGT 1 cut(s) 687
TaqI TCGA 5 cut(s) 170, 286, 639, 816, 919
TasI AATT 4 cut(s) 573, 641, 1386, 1650
TatI WGTACW 1 cut(s) 252
TauI GCSGC 2 cut(s) 212, 1021
TfiI GAWTC 5 cut(s) 361, 806, 874, 1164, 1604
Tru1I TTAA 6 cut(s) 237, 581, 761, 792, 972, 1047
Tru9I TTAA 6 cut(s) 237, 581, 761, 792, 972, 1047
TscAI CASTG 8 cut(s) 18, 712, 826, 1099, 1123, 1380, 1446, 1599
TseFI GTSAC 2 cut(s) 734, 1423
Tsp45I GTSAC 2 cut(s) 734, 1423
TspDTI ATGAA 7 cut(s) 174, 263, 908, 923, 1207, 1231, 1353
TspRI CASTG 8 cut(s) 18, 712, 826, 1099, 1123, 1380, 1446, 1599
Vha464I CTTAAG 1 cut(s) 971
VpaK11BI GGWCC 1 cut(s) 346
XagI CCTNNNNNAGG 1 cut(s) 353
XceI RCATGY 1 cut(s) 719
XcmI CCANNNNNNNNNTGG 1 cut(s) 1072
XspI CTAG 3 cut(s) 656, 860, 1208
ZrmI AGTACT 1 cut(s) 254
Zsp2I ATGCAT 2 cut(s) 304, 1035
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.