Rw3G013140

ankyrin repeat-containing protein At5g02620-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0004020
Physical Location & Seq
Forward (+)
13013084 .. 13021730
8647 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G013140.1

Sequence Viewer

Length: 3468 bp
ATGCTGAATGTCCGTTTGAGAACGGGACAAGATGCAGGTGAGAGGGACCTGGAGAAGGGGCTGCTGGAGCCCTCAACGCCGGGAACTCGATCGGAGCCGCCGATGTCACCGCACACATCGAGGCTGGCGCCGTTTTTGTCCAATTCCAGCAAGTCTCTGCTGGTTTCGAATTCTAGCAAGTCTCTGCTGGTTTCGAATTCCGGCAAGTCTCTGATGGTTTCGAATTCCAGTAAGTCTCTGGTTATGTCGAATTCCGGCAAGCGCTTGGATCAGAAGAAGAAGTACGTGCGGCAGGTGACCGGCCGCTTCAATGACACGGAGCTCCACCTGGCGGCGAAGAGGGGCGACGTCGCCGCCGTGAAGCATATCATCGGCGAGATTGATGCACAGATGATGGGGACCACGAGTGGGGCTGATTTTGATGCGGAGGTGGCTGAGATTAGGTCTGCGGTTGTGAATGAGGTCAATGAGTTGGGAGAGACGCCGCTTCTCGCCGCCGCCGAGAAGGGGCACCTTGGTGTGGTGAAGGAGCTCTTGCCTTTCACCACCGATGAGGGGATTTACATGAAGAATAGGATCGGCTACAATCCATTGCATATTGCTGCTAGTCAAGGGCACCATGCCATTATCCAGGTCCTGTTAGACCATGAACCAGGACTTAGCAAAACACTTGGCCCGTCAAATGCAACAGCTCTTGTGGCTGCAGCTACGAGAGGGCATACAGATGTGGTTAAGGAGTTAATGAATCATGATCCTAGCTTGGTAGAAATTTCTAAATCCAATGGTAAGAATGCATTGCATTTTGCGGCACGGCAGGGCCATGTAGATATTGTGAAAGCTTTACTTGACAAGGATCCACAACTAGTAAGAAGAATTGACAAGAAAGGACAGACTGCTTTGCATATGGCCGTAAAAGGTCATAGCTGTGAGGTGGTGAAGTTGCTACTTGACGCAGATTCAGCTCTTGTCATGCTCCCAGACAAGTTCGGTAATTTACCATTACATGTGGCCACGAGGAAGAAGAGAACAGAGATAGTAAATGAGTTGTTAGCCCTTCGTGACACAAATGTGAATGCTTTAACAAGAGAACAGAAAACACCCCTTGATATAGTTGAAGCACTTCCTCTCTCTGAAGAATCCACGGCAATTAAGGAAAGCTTAACTGAGTATGGTGGTCTCCGAGCCCAGGACCTTAACCGGCCAAGAGATGAGCTTAGAAACACAGTGTCAGCTATAAAAAAAGATGTCCACACTCAGCTGGATCAAGCCCGCAAGACCAGTCGGAGTGTTACTGGAATTGCCAAGGAGATCCGTAGGCTACAAAGGGAAGGAGTCAATATTGCAACTAACTCTGTCACAGTGGTTGCATCGCTCATCGCAACAATAGCATTTGCAGCTATATTTACTGTACCTGGTGGTGATTTAGATTCTGGGAAGTCTGTGACGGTTTCAACTACATCGTTCAAGATGTTTTATTTTTTTAATGCTATTTCACTCTTCGTAGCATTGGCTGTTGTCGTGGTACAGATCACCCTTATTCGGGGGGAGACGAAGACTGAGAGGAGGGTTATAACAGTGATTAATAAGGCGATGTGGTTGGCCTCTATGACCACTACAGTAGCATTTATTTCTGCAGCATATATAGTGGTTGGTCGACATAATCGGTGGGCCGCAATCTTTATCACAGTTATAGGAGGGATTATAATTTCAGCCGTTCTTTGTATCATGACATATTTTGTGATAAAGTCCAAAAAGATTCGTAGATTGAGAAAGAGAGAGAAGCTTCTTACAAGGACTGGATCCTCCTTGCACTCAGACTCCGGTTCAGAAGTGAATCCACCTGTGAGGCAGCAAAGCTTCTGCGAGAAGAAGATGACAAAGCAGTTCACAGGGAAGCGCGAAGATGCCCCCTTGCATTTGGCAGCAAGAGCAGGAAATTTGGGATTGGTGTTAGAAATCATCTCAAATGGTGGGGATGCAGAGTTGAATGAAGTGTTGATGAAGCAGAATCACTCGGGTGAAACCGCCTTGTATGTGGCGGCTGAGTGTGGCTATGTTGATTTGGTGAAGGAAATGATCAAGTACTGTGATGTTGGTTTAGCTAGCATCAAAGCTAGAAATGGCTGTGATGCATTTCACATTGCTTCCAAGCAAGGTCACTTGGAGGTATTGAAGGTTCTCATGAAGGCAATACCAGAACTTTCAATGACTGTTGATCAAACAAACACCACAGCGTTGCACGCCGCAGCAGCGCAGGGTCACATTGAAGTAGTAAATTTCCTTTTGGAGAATGGTTGCAGCTTGCTGACCATAGCAAGAAGCAATGCAAAAACCGCCTTTCATTCTGCAGCAAGAAATGGGCATTTAGATGTCGTCAAGGCACTCTTGAGAAAAGGACCTGAAATTGCAACGAGAAAGGATAAGAAGGGGCAGACAGCTCTCCATATGGCAGTTAAGGGACAGAGTGTTGAGCTGGTGGATGAGTTAGCAAAAGCGGATCCTTCTATGATAAATATGATGGATAACAAGGGCAACACTGCCTTGCATATAGCAACCCGGAAGGGTCGAGCTCAGATTGTTCAGCAGCTGTTGGGTTATAAGGGAATTGCCACAAGTACCATTAACAACTCTGGTGAAACTGTTCTTGACACTGCACAGAAAAATGGGCAATCTGTGATTGCTGTCATTCTAGAAGAGAAAGGCATTCAAAGTGCCAAATCCATGAAACCACCAACCATAATCGCAAACCGAGAGCTCAAACAAACTGTCAGTGACATAAAACATGAGGTGCATGACCAGATCAAGCAAACACGACATACACGAAAGCAAGTACATGGTATGGTAAAACGGCTCAGCAAAATGCACTCAGATGGGCTTAACAACGCAATAAACTCCACCACTGTTGTGGCTGTCCTCATTGCCACCGTTGCCTTTGCTGCAATTTTCACTGTCCCAGGCCAATACCCCCAAGACCAGGATAATCTTCCACCTGGATACTCTCCTGGAGAAGCAAACATTGCCCCAAAAGCCGGGTTCATAGTCTTCTTCATCTTTGATTCTTTTGCACTCTTCATATCATTGGCTGTAGTGGTGGTTCAGACTTCTATTGTTGTTATAGAGAGGAAGCAAAAAGAGAAACTGATGACAGTTATAAACAAGCTGATGTGGATGGCTTGTGTGATGGTTTCGGTTGCATTTCTTGCACTGTCTTATGTAATCGTCGGAGAGAAAGAGAAATGGTTGGCTGTTGGAGTAACAGGTATAGGTACAGTGATTATGGGAATGACATTGGGAACAATGTGTTATTGGATGGTTGTGCAGAGAATTGAGGCTTCAAAACTTCGTAGCATCACTCGGAGATCATCAATGAGCACTAGGTCACACTCACATTCTGGGTCTTTCTCAGTGATGTCAGACTCTGAGCTTATCAACACTGAGTTTAAGAAGAAGAAAGTGTATGCAATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005886 GO:0012505 GO:0016020 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 Ank_3 Ank_4 Ank_5 PGG

Protein Analysis

1155

Amino Acids

125.76

Weight (kDa)

9.57

Isoelectric Point (pI)

35.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 108 - 217 2.4e-13 Ankyrin repeats (3 copies)
Ank_4 PF13637 163 - 214 3.9e-07 Ankyrin repeats (many copies)
Ank_2 PF12796 227 - 286 6.8e-13 Ankyrin repeats (3 copies)
Ank_4 PF13637 233 - 282 1e-07 Ankyrin repeats (many copies)
Ank_4 PF13637 266 - 316 4.5e-10 Ankyrin repeats (many copies)
Ank_5 PF13857 281 - 318 8.2e-06 Ankyrin repeats (many copies)
Ank_2 PF12796 292 - 358 1.4e-09 Ankyrin repeats (3 copies)
PGG PF13962 445 - 549 3.7e-21 Domain of unknown function
Ank_2 PF12796 633 - 698 3.6e-07 Ankyrin repeats (3 copies)
Ank_2 PF12796 705 - 768 2.4e-11 Ankyrin repeats (3 copies)
Ank_4 PF13637 713 - 762 9.7e-07 Ankyrin repeats (many copies)
Ank_4 PF13637 746 - 796 8.9e-09 Ankyrin repeats (many copies)
Ank_2 PF12796 775 - 834 9.9e-11 Ankyrin repeats (3 copies)
Ank_4 PF13637 781 - 826 1.4e-08 Ankyrin repeats (many copies)
Ank_5 PF13857 795 - 851 1.3e-07 Ankyrin repeats (many copies)
Ank_2 PF12796 836 - 902 4.7e-08 Ankyrin repeats (3 copies)
PGG PF13962 958 - 1072 1.9e-29 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 4 cut(s) 1571, 1703, 2598, 3152
AarI CACCTGC 2 cut(s) 26, 283
AatII GACGTC 1 cut(s) 351
Acc36I ACCTGC 2 cut(s) 26, 283
AccB1I GGYRCC 3 cut(s) 127, 510, 615
AccI GTMKAC 1 cut(s) 1654
AccII CGCG 1 cut(s) 1899
AcoI YGGCCR 4 cut(s) 301, 906, 1008, 1199
AcsI RAATTY 7 cut(s) 169, 196, 223, 250, 768, 1936, 2275
AcuI CTGAAG 1 cut(s) 1152
AcyI GRCGYC 3 cut(s) 128, 348, 482
AfaI GTAC 7 cut(s) 284, 1410, 1524, 2084, 2617, 2832, 3268
AfeI AGCGCT 1 cut(s) 263
AflIII ACRYGT 1 cut(s) 1003
AhlI ACTAGT 1 cut(s) 862
AleI CACNNNNGTG 4 cut(s) 516, 1067, 2016, 2903
Alw21I GWGCWC 5 cut(s) 324, 534, 2572, 2757, 3374
Alw26I GTCTC 7 cut(s) 159, 186, 213, 240, 473, 1181, 1541
AlwNI CAGNNNCTG 3 cut(s) 637, 2587, 3419
Ama87I CYCGRG 1 cut(s) 2014
Aor51HI AGCGCT 1 cut(s) 263
AoxI GGCC 9 cut(s) 301, 673, 817, 906, 1008, 1199, 1599, 1668, 2956
ApoI RAATTY 7 cut(s) 169, 196, 223, 250, 768, 1936, 2275
ArsI GACNNNNNNTTYG 2 cut(s) 2751, 2783
AseI ATTAAT 1 cut(s) 1581
Asp700I GAANNNNTTC 2 cut(s) 1119, 2640
AspLEI GCGC 4 cut(s) 130, 264, 1899, 2254
AspS9I GGNCC 8 cut(s) 46, 399, 634, 674, 817, 1189, 1668, 2396
AsuC2I CCSGG 3 cut(s) 81, 2557, 3031
AsuII TTCGAA 3 cut(s) 167, 194, 221
AsuNHI GCTAGC 1 cut(s) 2102
AvaI CYCGRG 1 cut(s) 2014
AvaII GGWCC 5 cut(s) 46, 399, 634, 1189, 2396
BaeGI GKGCMC 2 cut(s) 513, 618
BalI TGGCCA 1 cut(s) 1010
BamHI GGATCC 3 cut(s) 853, 1799, 2497
BanI GGYRCC 3 cut(s) 127, 510, 615
BanII GRGCYC 6 cut(s) 72, 324, 534, 1186, 2572, 2757
BarI GAAGNNNNNNTAC 2 cut(s) 266, 298
BauI CACGAG 2 cut(s) 403, 1012
BbsI GAAGAC 2 cut(s) 1559, 3034
Bbv12I GWGCWC 5 cut(s) 324, 534, 2572, 2757, 3374
BccI CCATC 7 cut(s) 208, 388, 2512, 2864, 3163, 3175, 3304
BceAI ACGGC 7 cut(s) 115, 341, 827, 893, 1158, 1697, 2864
BcgI CGANNNNNNTGC 2 cut(s) 365, 399
BciVI GTATCC 1 cut(s) 2987
BclI TGATCA 2 cut(s) 2076, 2215
BcnI CCSGG 3 cut(s) 81, 2557, 3031
BcoDI GTCTC 7 cut(s) 159, 186, 213, 240, 473, 1181, 1541
BcuI ACTAGT 1 cut(s) 862
BfaI CTAG 8 cut(s) 174, 606, 756, 863, 2103, 2115, 2690, 3375
BfmI CTRYAG 5 cut(s) 702, 1614, 1632, 2346, 3084
BfoI RGCGCY 2 cut(s) 131, 265
BfuAI ACCTGC 2 cut(s) 26, 283
BfuI GTATCC 1 cut(s) 2987
BlpI GCTNAGC 1 cut(s) 2852
BmcAI AGTACT 1 cut(s) 2084
Bme18I GGWCC 5 cut(s) 46, 399, 634, 1189, 2396
BmeT110I CYCGRG 1 cut(s) 2014
BmgT120I GGNCC 8 cut(s) 46, 399, 634, 674, 817, 1189, 1668, 2396
BmsI GCATC 9 cut(s) 22, 373, 412, 1376, 1894, 1966, 2115, 2119, 3357
BmtI GCTAGC 1 cut(s) 2106
BpiI GAAGAC 2 cut(s) 1559, 3034
BpmI CTGGAG 3 cut(s) 71, 86, 3024
Bpu1102I GCTNAGC 1 cut(s) 2852
Bpu14I TTCGAA 3 cut(s) 167, 194, 221
BpuEI CTTGAG 1 cut(s) 2407
BpuMI CCSGG 3 cut(s) 81, 2557, 3031
BsaAI YACGTR 1 cut(s) 286
BsaHI GRCGYC 3 cut(s) 128, 348, 482
BsaI GGTCTC 1 cut(s) 1181
BsaJI CCNNGG 5 cut(s) 514, 1140, 1185, 1302, 2953
BsaWI WCCGGW 1 cut(s) 1820
BsaXI ACNNNNNCTCC 2 cut(s) 1802, 1832
Bse118I RCCGGY 2 cut(s) 299, 1197
Bse1I ACTGG 4 cut(s) 228, 1278, 1297, 1801
Bse3DI GCAATG 6 cut(s) 590, 794, 2139, 2329, 2916, 3015
BseDI CCNNGG 5 cut(s) 514, 1140, 1185, 1302, 2953
BseGI GGATG 4 cut(s) 1981, 2485, 3174, 3315
BseMI GCAATG 6 cut(s) 590, 794, 2139, 2329, 2916, 3015
BseNI ACTGG 4 cut(s) 228, 1278, 1297, 1801
BseRI GAGGAG 1 cut(s) 1576
BseSI GKGCMC 2 cut(s) 513, 618
BseX3I CGGCCG 1 cut(s) 301
BsgI GTGCAG 2 cut(s) 2637, 3338
Bsh1236I CGCG 1 cut(s) 1899
Bsh1285I CGRYCG 2 cut(s) 92, 304
BshFI GGCC 9 cut(s) 303, 675, 819, 908, 1010, 1201, 1601, 1670, 2958
BshNI GGYRCC 3 cut(s) 127, 510, 615
BsiEI CGRYCG 2 cut(s) 92, 304
BsiHKAI GWGCWC 5 cut(s) 324, 534, 2572, 2757, 3374
BsiHKCI CYCGRG 1 cut(s) 2014
BsiSI CCGG 8 cut(s) 80, 201, 255, 300, 1198, 1821, 2557, 3030
BslFI GGGAC 5 cut(s) 39, 59, 412, 2472, 2936
BsmAI GTCTC 7 cut(s) 159, 186, 213, 240, 473, 1181, 1541
BsmBI CGTCTC 2 cut(s) 473, 1541
BsmFI GGGAC 5 cut(s) 39, 59, 412, 2472, 2936
BsmI GAATGC 3 cut(s) 796, 1078, 2703
BsnI GGCC 9 cut(s) 303, 675, 819, 908, 1010, 1201, 1601, 1670, 2958
Bso31I GGTCTC 1 cut(s) 1181
BsoBI CYCGRG 1 cut(s) 2014
Bsp119I TTCGAA 3 cut(s) 167, 194, 221
Bsp1286I GDGCHC 9 cut(s) 72, 324, 513, 534, 618, 1186, 2572, 2757, 3374
Bsp1720I GCTNAGC 1 cut(s) 2852
BspANI GGCC 9 cut(s) 303, 675, 819, 908, 1010, 1201, 1601, 1670, 2958
BspFNI CGCG 1 cut(s) 1899
BspHI TCATGA 3 cut(s) 748, 1725, 2181
BspMAI CTGCAG 3 cut(s) 706, 1636, 2350
BspMI ACCTGC 2 cut(s) 26, 283
BspOI GCTAGC 1 cut(s) 2106
BspT104I TTCGAA 3 cut(s) 167, 194, 221
BspT107I GGYRCC 3 cut(s) 127, 510, 615
BspTNI GGTCTC 1 cut(s) 1181
BsrDI GCAATG 6 cut(s) 590, 794, 2139, 2329, 2916, 3015
BsrFI RCCGGY 2 cut(s) 299, 1197
BsrI ACTGG 4 cut(s) 228, 1278, 1297, 1801
BssAI RCCGGY 2 cut(s) 299, 1197
BssECI CCNNGG 5 cut(s) 514, 1140, 1185, 1302, 2953
BssNI GRCGYC 3 cut(s) 128, 348, 482
BssSI CACGAG 2 cut(s) 403, 1012
BssT1I CCWWGG 2 cut(s) 514, 1302
Bst2BI CACGAG 2 cut(s) 403, 1012
Bst6I CTCTTC 5 cut(s) 332, 1016, 1502, 2688, 3074
BstACI GRCGYC 3 cut(s) 128, 348, 482
BstAPI GCANNNNNTGC 2 cut(s) 3017, 3200
BstBAI YACGTR 1 cut(s) 286
BstBI TTCGAA 3 cut(s) 167, 194, 221
BstC8I GCNNGC 6 cut(s) 126, 260, 1270, 2104, 2241, 2303
BstDSI CCRYGG 1 cut(s) 1140
BstEII GGTNACC 1 cut(s) 295
BstENI CCTNNNNNAGG 1 cut(s) 53
BstF5I GGATG 4 cut(s) 1981, 2485, 3174, 3315
BstFNI CGCG 1 cut(s) 1899
BstH2I RGCGCY 2 cut(s) 131, 265
BstHHI GCGC 4 cut(s) 130, 264, 1899, 2254
BstMAI GTCTC 7 cut(s) 159, 186, 213, 240, 473, 1181, 1541
BstMCI CGRYCG 2 cut(s) 92, 304
BstNSI RCATGY 1 cut(s) 1007
BstPI GGTNACC 1 cut(s) 295
BstSFI CTRYAG 5 cut(s) 702, 1614, 1632, 2346, 3084
BstSLI GKGCMC 2 cut(s) 513, 618
BstUI CGCG 1 cut(s) 1899
BstV2I GAAGAC 2 cut(s) 1559, 3034
BstX2I RGATCY 4 cut(s) 853, 1308, 1799, 2497
BstXI CCANNNNNNTGG 1 cut(s) 2905
BstYI RGATCY 4 cut(s) 853, 1308, 1799, 2497
BstZI CGGCCG 1 cut(s) 301
BsuI GTATCC 1 cut(s) 2987
BsuRI GGCC 9 cut(s) 303, 675, 819, 908, 1010, 1201, 1601, 1670, 2958
BtgI CCRYGG 1 cut(s) 1140
BtgZI GCGATG 3 cut(s) 1353, 1360, 1604
BtsCI GGATG 4 cut(s) 1981, 2485, 3174, 3315
BtsI GCAGTG 2 cut(s) 2535, 2649
BveI ACCTGC 2 cut(s) 26, 283
Cac8I GCNNGC 6 cut(s) 126, 260, 1270, 2104, 2241, 2303
CaiI CAGNNNCTG 3 cut(s) 637, 2587, 3419
CciI TCATGA 3 cut(s) 748, 1725, 2181
CfoI GCGC 4 cut(s) 130, 264, 1899, 2254
Cfr10I RCCGGY 2 cut(s) 299, 1197
Cfr13I GGNCC 8 cut(s) 46, 399, 634, 674, 817, 1189, 1668, 2396
CseI GACGC 2 cut(s) 490, 959
CsiI ACCWGGT 1 cut(s) 1411
Csp6I GTAC 7 cut(s) 283, 1409, 1523, 2083, 2616, 2831, 3267
CspCI CAANNNNNGTGG 2 cut(s) 2884, 2919
CviQI GTAC 7 cut(s) 283, 1409, 1523, 2083, 2616, 2831, 3267
DinI GGCGCC 1 cut(s) 129
EaeI YGGCCR 4 cut(s) 301, 906, 1008, 1199
EagI CGGCCG 1 cut(s) 301
Eam1104I CTCTTC 5 cut(s) 332, 1016, 1502, 2688, 3074
EarI CTCTTC 5 cut(s) 332, 1016, 1502, 2688, 3074
Ecl136II GAGCTC 4 cut(s) 322, 532, 2570, 2755
EclXI CGGCCG 1 cut(s) 301
Eco130I CCWWGG 2 cut(s) 514, 1302
Eco24I GRGCYC 6 cut(s) 72, 324, 534, 1186, 2572, 2757
Eco31I GGTCTC 1 cut(s) 1181
Eco47I GGWCC 5 cut(s) 46, 399, 634, 1189, 2396
Eco47III AGCGCT 1 cut(s) 263
Eco52I CGGCCG 1 cut(s) 301
Eco53kI GAGCTC 4 cut(s) 322, 532, 2570, 2755
Eco57I CTGAAG 1 cut(s) 1152
Eco88I CYCGRG 1 cut(s) 2014
Eco91I GGTNACC 1 cut(s) 295
EcoICRI GAGCTC 4 cut(s) 322, 532, 2570, 2755
EcoNI CCTNNNNNAGG 1 cut(s) 53
EcoO109I RGGNCCY 4 cut(s) 46, 634, 1189, 2396
EcoO65I GGTNACC 1 cut(s) 295
EcoRI GAATTC 4 cut(s) 169, 196, 223, 250
EcoT14I CCWWGG 2 cut(s) 514, 1302
EcoT22I ATGCAT 2 cut(s) 796, 2134
EcoT38I GRGCYC 6 cut(s) 72, 324, 534, 1186, 2572, 2757
EgeI GGCGCC 1 cut(s) 129
EheI GGCGCC 1 cut(s) 129
ErhI CCWWGG 2 cut(s) 514, 1302
Esp3I CGTCTC 2 cut(s) 473, 1541
FalI AAGNNNNNCTT 8 cut(s) 518, 550, 828, 860, 1142, 1174, 2369, 2401
FaqI GGGAC 5 cut(s) 39, 59, 412, 2472, 2936
FauI CCCGC 1 cut(s) 1277
FauNDI CATATG 2 cut(s) 903, 2445
FbaI TGATCA 2 cut(s) 2076, 2215
FblI GTMKAC 1 cut(s) 1654
FokI GGATG 4 cut(s) 1988, 2492, 3181, 3322
FriOI GRGCYC 6 cut(s) 72, 324, 534, 1186, 2572, 2757
FspBI CTAG 8 cut(s) 174, 606, 756, 863, 2103, 2115, 2690, 3375
GlaI GCGC 4 cut(s) 129, 263, 1898, 2253
GsuI CTGGAG 3 cut(s) 71, 86, 3024
HaeII RGCGCY 2 cut(s) 131, 265
HaeIII GGCC 9 cut(s) 303, 675, 819, 908, 1010, 1201, 1601, 1670, 2958
HapII CCGG 8 cut(s) 80, 201, 255, 300, 1198, 1821, 2557, 3030
HgaI GACGC 2 cut(s) 490, 959
HhaI GCGC 4 cut(s) 130, 264, 1899, 2254
Hin1I GRCGYC 3 cut(s) 128, 348, 482
Hin6I GCGC 4 cut(s) 128, 262, 1897, 2252
HinP1I GCGC 4 cut(s) 128, 262, 1897, 2252
HincII GTYRAC 1 cut(s) 1655
HindII GTYRAC 1 cut(s) 1655
HindIII AAGCTT 4 cut(s) 837, 1156, 1781, 1855
HpaII CCGG 8 cut(s) 80, 201, 255, 300, 1198, 1821, 2557, 3030
Hpy166II GTNNAC 3 cut(s) 1249, 1655, 1887
Hpy188III TCNNGA 8 cut(s) 749, 1058, 1465, 1726, 2182, 2386, 2645, 2690
Hpy8I GTNNAC 3 cut(s) 1249, 1655, 1887
Hpy99I CGWCG 3 cut(s) 350, 353, 3224
HpyCH4IV ACGT 2 cut(s) 285, 348
HpySE526I ACGT 2 cut(s) 285, 348
Hsp92I GRCGYC 3 cut(s) 128, 348, 482
HspAI GCGC 4 cut(s) 128, 262, 1897, 2252
KasI GGCGCC 1 cut(s) 127
Ksp22I TGATCA 2 cut(s) 2076, 2215
LmnI GCTCC 6 cut(s) 67, 94, 319, 327, 529, 978
LweI GCATC 9 cut(s) 22, 373, 412, 1376, 1894, 1966, 2115, 2119, 3357
MabI ACCWGGT 1 cut(s) 1411
MaeI CTAG 8 cut(s) 174, 606, 756, 863, 2103, 2115, 2690, 3375
MaeII ACGT 2 cut(s) 285, 348
MflI RGATCY 4 cut(s) 853, 1308, 1799, 2497
MhlI GDGCHC 9 cut(s) 72, 324, 513, 534, 618, 1186, 2572, 2757, 3374
MlsI TGGCCA 1 cut(s) 1010
MluNI TGGCCA 1 cut(s) 1010
Mly113I GGCGCC 1 cut(s) 128
MlyI GAGTC 3 cut(s) 1341, 1811, 3410
MmeI TCCRAC 3 cut(s) 1262, 3202, 3229
Mox20I TGGCCA 1 cut(s) 1010
Mph1103I ATGCAT 2 cut(s) 796, 2134
MroXI GAANNNNTTC 2 cut(s) 1119, 2640
MscI TGGCCA 1 cut(s) 1010
MslI CAYNNNNRTG 8 cut(s) 516, 723, 924, 1067, 2016, 2367, 2868, 2903
Msp20I TGGCCA 1 cut(s) 1010
MspA1I CMGCKG 2 cut(s) 1258, 2587
MspI CCGG 8 cut(s) 80, 201, 255, 300, 1198, 1821, 2557, 3030
Mva1269I GAATGC 3 cut(s) 796, 1078, 2703
MvnI CGCG 1 cut(s) 1899
NarI GGCGCC 1 cut(s) 128
NciI CCSGG 3 cut(s) 81, 2557, 3031
NdeI CATATG 2 cut(s) 903, 2445
NheI GCTAGC 1 cut(s) 2102
NmeAIII GCCGAG 1 cut(s) 526
NmuCI GTSAC 9 cut(s) 105, 295, 1058, 1354, 1441, 2156, 2258, 2771, 3378
NsiI ATGCAT 2 cut(s) 796, 2134
NspI RCATGY 1 cut(s) 1007
NspV TTCGAA 3 cut(s) 167, 194, 221
OliI CACNNNNGTG 4 cut(s) 516, 1067, 2016, 2903
PagI TCATGA 3 cut(s) 748, 1725, 2181
PaqCI CACCTGC 2 cut(s) 26, 283
PciI ACATGT 1 cut(s) 1003
PcsI WCGNNNNNNNCGW 4 cut(s) 98, 354, 498, 2818
PctI GAATGC 3 cut(s) 796, 1078, 2703
PdmI GAANNNNTTC 2 cut(s) 1119, 2640
PfeI GAWTC 8 cut(s) 745, 956, 1136, 1427, 1756, 1834, 2008, 3056
PfoI TCCNGGA 1 cut(s) 3001
Ple19I CGATCG 1 cut(s) 92
PleI GAGTC 3 cut(s) 1340, 1811, 3410
PluTI GGCGCC 1 cut(s) 131
PpsI GAGTC 3 cut(s) 1340, 1811, 3410
Ppu21I YACGTR 1 cut(s) 286
PpuMI RGGWCCY 4 cut(s) 46, 634, 1189, 2396
PscI ACATGT 1 cut(s) 1003
PshBI ATTAAT 1 cut(s) 1581
PsiI TTATAA 4 cut(s) 1571, 1703, 2598, 3152
Psp124BI GAGCTC 4 cut(s) 324, 534, 2572, 2757
Psp5II RGGWCCY 4 cut(s) 46, 634, 1189, 2396
PspEI GGTNACC 1 cut(s) 295
PspPI GGNCC 8 cut(s) 46, 399, 634, 674, 817, 1189, 1668, 2396
PspPPI RGGWCCY 4 cut(s) 46, 634, 1189, 2396
PsrI GAACNNNNNNTAC 4 cut(s) 2160, 2192, 3078, 3110
PstI CTGCAG 3 cut(s) 706, 1636, 2350
PstNI CAGNNNCTG 3 cut(s) 637, 2587, 3419
PsuI RGATCY 4 cut(s) 853, 1308, 1799, 2497
PvuI CGATCG 1 cut(s) 92
PvuII CAGCTG 2 cut(s) 1258, 2587
RsaI GTAC 7 cut(s) 284, 1410, 1524, 2084, 2617, 2832, 3268
RsaNI GTAC 7 cut(s) 283, 1409, 1523, 2083, 2616, 2831, 3267
RseI CAYNNNNRTG 8 cut(s) 516, 723, 924, 1067, 2016, 2367, 2868, 2903
SacI GAGCTC 4 cut(s) 324, 534, 2572, 2757
SalI GTCGAC 1 cut(s) 1653
Sau96I GGNCC 8 cut(s) 46, 399, 634, 674, 817, 1189, 1668, 2396
ScaI AGTACT 1 cut(s) 2084
SchI GAGTC 3 cut(s) 1341, 1811, 3410
SduI GDGCHC 9 cut(s) 72, 324, 513, 534, 618, 1186, 2572, 2757, 3374
SexAI ACCWGGT 1 cut(s) 1411
SfaNI GCATC 9 cut(s) 22, 373, 412, 1376, 1894, 1966, 2115, 2119, 3357
SfcI CTRYAG 5 cut(s) 702, 1614, 1632, 2346, 3084
SfoI GGCGCC 1 cut(s) 129
SfuI TTCGAA 3 cut(s) 167, 194, 221
SinI GGWCC 5 cut(s) 46, 399, 634, 1189, 2396
SmiMI CAYNNNNRTG 8 cut(s) 516, 723, 924, 1067, 2016, 2367, 2868, 2903
SmlI CTYRAG 1 cut(s) 2386
SmoI CTYRAG 1 cut(s) 2386
SpeI ACTAGT 1 cut(s) 862
SspDI GGCGCC 1 cut(s) 127
SspI AATATT 1 cut(s) 1339
SspMI CTAG 8 cut(s) 174, 606, 756, 863, 2103, 2115, 2690, 3375
SstI GAGCTC 4 cut(s) 324, 534, 2572, 2757
StyI CCWWGG 2 cut(s) 514, 1302
TaiI ACGT 2 cut(s) 288, 351
TaqI TCGA 8 cut(s) 88, 119, 167, 194, 221, 248, 1654, 2566
TatI WGTACW 2 cut(s) 2082, 2830
TfiI GAWTC 8 cut(s) 745, 956, 1136, 1427, 1756, 1834, 2008, 3056
TseFI GTSAC 9 cut(s) 105, 295, 1058, 1354, 1441, 2156, 2258, 2771, 3378
Tsp45I GTSAC 9 cut(s) 105, 295, 1058, 1354, 1441, 2156, 2258, 2771, 3378
TspGWI ACGGA 2 cut(s) 332, 1301
VpaK11BI GGWCC 5 cut(s) 46, 399, 634, 1189, 2396
VspI ATTAAT 1 cut(s) 1581
XagI CCTNNNNNAGG 1 cut(s) 53
XapI RAATTY 7 cut(s) 169, 196, 223, 250, 768, 1936, 2275
XbaI TCTAGA 1 cut(s) 2689
XceI RCATGY 1 cut(s) 1007
XcmI CCANNNNNNNNNTGG 2 cut(s) 235, 2902
XmiI GTMKAC 1 cut(s) 1654
XmnI GAANNNNTTC 2 cut(s) 1119, 2640
XspI CTAG 8 cut(s) 174, 606, 756, 863, 2103, 2115, 2690, 3375
ZraI GACGTC 1 cut(s) 349
ZrmI AGTACT 1 cut(s) 2084
Zsp2I ATGCAT 2 cut(s) 796, 2134
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.