Rh3BG160200

ankyrin repeat-containing protein At5g02620-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0004020
Physical Location & Seq
Forward (+)
13827265 .. 13830678
3414 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG160200.1

Sequence Viewer

Length: 1596 bp
ATGACAAAGCAGTTGACAGGGAAGCGCGAAGATGCCCCCTTGCATTTGGCAGCAAGAGCAGGAAATTTGGGATTGGTGTTAGAAATCATCTCAAATGGTGGGGATGCAGAGTTGAATGAAGTGTTGATGAAGCAGAATCACTCGGGTGAAACCGCCTTGTATGTGGCGGCTGAGTGTGGCTATGTTGATTTGGTGAAGGAAATGATCAAGTACTGTGATGTTGGTTTAGCTAGCATCAAAGCTAGAAATGGCTGTGATGCATTTCACATTGCTTCCAAGCAAGGTCACTTGGAGGTATTGAAGGTTCTCATGAAGGCAATACCAGAACTTTCAATGACTGTTGATCAAACAAACACCACAGCGTTGCACGCCGCAGCAGCGCAGGGTCACATTGAAGTAGTAAATTTCCTTTTGGAGAATGGTTGCAGCTTGCTGACCATAGCAAGAAGCAATGCAAAAACCGCCTTTCATTCTGCAGCAAGAAATGGGCATTTAGATGTCGTCAAGGCACTCTTGAGAAAAGGACCTGAAATTGCAACGAGAAAGGATAAGAAGGGGCAGACAGCTCTCCATATGGCAGTTAAGGGACAGAATGTTGAGCTGGTGGATGAGTTAGCAAAAGCGGATCCTTCTATGATAAATATGATGGATAACAAGGGCAACACTGCCTTGCATATAGCAACCCGGAAGGGTCGAGCTCAGATTGTTCAGCAGCTGTTGGGTTATAAGGGAATTGCCACAAGTACCATTAACAACTCTGGTGAAACTGTTCTTGACACTGCACAGAAAAATGGGCAATCTGTGATTGCTGCCATTCTAGAAGAGAAAGGCATTCAAAGTGCCAAATCCATGAAACCACCAACCATAATCGCAAACCGAGAGCTCAAACAAACTGTCAGTGACATAAAACATGAGGTGCATGACCAGATCAAGCAAACACGACATACACGAAAGCAAGTACATGGAATGGTAAAACGGCTGAGCAAAATGCACTCAGATGGGCTTAACAACGCAATAAACTCCACCACTGTTGTGGCTGTCCTCATTGCCACCGTTGCCTTTGCTGCAATTTTCACTGTCCCAGGCCAATACCCCAATGACCAGGATAATCTTCCACCTGGATACTCTCCTGGAGAAGCAAACATTGCCCCAAAAGCCGGGTTCATAGTCTTCTTCATCTTTGATTCTTTTGCACTCTTCATATCATTGGCTGTAGTGGTGGTTCAGACTTCTATTGTTGTTATAGAGAGGAAGCAAAAAGAGAAACTGATGACAGTTATAAACAAGCTGATGTGGATGGCTTGTGTGATGGTTTCGGTTGCATTTCTTGCACTGTCTTATGTAATCGTCGGAGAGAAAGAGAAATGGTTGGCTGTTGGAGTAACAGGCATAGGTACAGTGATTATGGGAATGACATTGGGAACAATGTGTTATTGGATGGTTGTGCAGAGAATTGAGGCTTCAAAACTTCGTAGCATCACTCGGAGATCATCAATGAGCACTAGGTCACACTCACATTCTGGGTCTTTCTCAGTGATGTCAGACTCTGAGCTTATCAACACTGAGTTCAAGAAGAAGAAAGTGTATGCAATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005886 GO:0012505 GO:0016020 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 Ank_3 Ank_4 Ank_5 PGG

Protein Analysis

531

Amino Acids

57.72

Weight (kDa)

9.37

Isoelectric Point (pI)

32.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 9 - 74 2.5e-07 Ankyrin repeats (3 copies)
Ank_2 PF12796 81 - 144 8.6e-12 Ankyrin repeats (3 copies)
Ank_4 PF13637 89 - 138 3.8e-07 Ankyrin repeats (many copies)
Ank_4 PF13637 122 - 172 3.4e-09 Ankyrin repeats (many copies)
Ank_2 PF12796 151 - 210 2.1e-11 Ankyrin repeats (3 copies)
Ank_4 PF13637 157 - 202 1.2e-09 Ankyrin repeats (many copies)
Ank_5 PF13857 171 - 227 2.8e-08 Ankyrin repeats (many copies)
Ank_2 PF12796 199 - 278 3.3e-09 Ankyrin repeats (3 copies)
PGG PF13962 334 - 448 1.4e-29 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 726, 1280
AccII CGCG 1 cut(s) 27
AciI CCGC 5 cut(s) 153, 167, 372, 462, 623
AclWI GGATC 2 cut(s) 620, 633
AcsI RAATTY 2 cut(s) 64, 403
AfaI GTAC 4 cut(s) 212, 745, 960, 1396
AfiI CCNNNNNNNGG 1 cut(s) 1157
AgsI TTSAA 7 cut(s) 115, 301, 333, 395, 836, 1464, 1570
AjnI CCWGG 4 cut(s) 1081, 1101, 1117, 1129
AleI CACNNNNGTG 2 cut(s) 144, 1031
Alw21I GWGCWC 3 cut(s) 700, 885, 1502
AlwI GGATC 2 cut(s) 620, 633
AlwNI CAGNNNCTG 2 cut(s) 715, 1547
Ama87I CYCGRG 1 cut(s) 142
AoxI GGCC 1 cut(s) 1084
ApeKI GCWGC 8 cut(s) 50, 374, 377, 426, 476, 712, 809, 1064
ApoI RAATTY 2 cut(s) 64, 403
ArsI GACNNNNNNTTYG 2 cut(s) 879, 911
Asp700I GAANNNNTTC 1 cut(s) 768
AspLEI GCGC 2 cut(s) 27, 382
AspS9I GGNCC 1 cut(s) 524
AsuC2I CCSGG 2 cut(s) 685, 1159
AsuHPI GGTGA 3 cut(s) 158, 205, 773
AsuNHI GCTAGC 1 cut(s) 230
AvaI CYCGRG 1 cut(s) 142
AvaII GGWCC 1 cut(s) 524
BamHI GGATCC 1 cut(s) 625
BanII GRGCYC 2 cut(s) 700, 885
BbsI GAAGAC 1 cut(s) 1162
Bbv12I GWGCWC 3 cut(s) 700, 885, 1502
BbvI GCAGC 8 cut(s) 62, 386, 389, 438, 488, 724, 796, 1051
BccI CCATC 5 cut(s) 640, 992, 1291, 1303, 1432
BceAI ACGGC 1 cut(s) 992
BciT130I CCWGG 4 cut(s) 1083, 1103, 1119, 1131
BciVI GTATCC 1 cut(s) 1115
BclI TGATCA 2 cut(s) 204, 343
BcnI CCSGG 2 cut(s) 685, 1159
BfaI CTAG 4 cut(s) 231, 243, 818, 1503
BfmI CTRYAG 2 cut(s) 474, 1212
BfuI GTATCC 1 cut(s) 1115
BlpI GCTNAGC 1 cut(s) 980
BmcAI AGTACT 1 cut(s) 212
Bme1390I CCNGG 6 cut(s) 685, 1083, 1103, 1119, 1131, 1159
Bme18I GGWCC 1 cut(s) 524
BmeT110I CYCGRG 1 cut(s) 142
BmgT120I GGNCC 1 cut(s) 524
BmiI GGNNCC 1 cut(s) 627
BmrFI CCNGG 6 cut(s) 685, 1083, 1103, 1119, 1131, 1159
BmsI GCATC 5 cut(s) 22, 94, 243, 247, 1485
BmtI GCTAGC 1 cut(s) 234
BpiI GAAGAC 1 cut(s) 1162
BpmI CTGGAG 1 cut(s) 1152
Bpu1102I GCTNAGC 1 cut(s) 980
BpuEI CTTGAG 1 cut(s) 535
BpuMI CCSGG 2 cut(s) 685, 1159
BsaJI CCNNGG 1 cut(s) 1081
Bsc4I CCNNNNNNNGG 1 cut(s) 1157
Bse3DI GCAATG 4 cut(s) 267, 457, 1044, 1143
BseBI CCWGG 4 cut(s) 1083, 1103, 1119, 1131
BseDI CCNNGG 1 cut(s) 1081
BseGI GGATG 4 cut(s) 109, 613, 1302, 1443
BseLI CCNNNNNNNGG 1 cut(s) 1157
BseMI GCAATG 4 cut(s) 267, 457, 1044, 1143
BseMII CTCAG 7 cut(s) 162, 713, 971, 1008, 1539, 1545, 1554
BseXI GCAGC 8 cut(s) 62, 386, 389, 438, 488, 724, 796, 1051
BsgI GTGCAG 2 cut(s) 765, 1466
Bsh1236I CGCG 1 cut(s) 27
BshFI GGCC 1 cut(s) 1086
BsiHKAI GWGCWC 3 cut(s) 700, 885, 1502
BsiHKCI CYCGRG 1 cut(s) 142
BsiSI CCGG 2 cut(s) 685, 1158
BslFI GGGAC 2 cut(s) 600, 1064
BslI CCNNNNNNNGG 1 cut(s) 1157
BsmFI GGGAC 2 cut(s) 600, 1064
BsmI GAATGC 1 cut(s) 831
BsnI GGCC 1 cut(s) 1086
BsoBI CYCGRG 1 cut(s) 142
Bsp1286I GDGCHC 3 cut(s) 700, 885, 1502
Bsp143I GATC 5 cut(s) 204, 343, 625, 927, 1487
Bsp1720I GCTNAGC 1 cut(s) 980
BspACI CCGC 5 cut(s) 153, 167, 372, 462, 623
BspANI GGCC 1 cut(s) 1086
BspCNI CTCAG 7 cut(s) 163, 712, 972, 1007, 1540, 1544, 1555
BspFNI CGCG 1 cut(s) 27
BspHI TCATGA 1 cut(s) 309
BspLI GGNNCC 1 cut(s) 627
BspMAI CTGCAG 1 cut(s) 478
BspOI GCTAGC 1 cut(s) 234
BspPI GGATC 2 cut(s) 620, 633
BsrDI GCAATG 4 cut(s) 267, 457, 1044, 1143
BssECI CCNNGG 1 cut(s) 1081
BssMI GATC 5 cut(s) 204, 343, 625, 927, 1487
Bst2UI CCWGG 4 cut(s) 1083, 1103, 1119, 1131
Bst6I CTCTTC 2 cut(s) 816, 1202
BstAPI GCANNNNNTGC 2 cut(s) 1145, 1328
BstC8I GCNNGC 3 cut(s) 232, 369, 431
BstDEI CTNAG 7 cut(s) 171, 699, 980, 994, 1531, 1548, 1563
BstF5I GGATG 4 cut(s) 109, 613, 1302, 1443
BstFNI CGCG 1 cut(s) 27
BstHHI GCGC 2 cut(s) 27, 382
BstKTI GATC 5 cut(s) 207, 346, 628, 930, 1490
BstMBI GATC 5 cut(s) 204, 343, 625, 927, 1487
BstMWI GCNNNNNNNGC 9 cut(s) 56, 368, 377, 461, 1055, 1064, 1145, 1154, 1328
BstNI CCWGG 4 cut(s) 1083, 1103, 1119, 1131
BstSCI CCNGG 6 cut(s) 683, 1081, 1101, 1117, 1129, 1157
BstSFI CTRYAG 2 cut(s) 474, 1212
BstUI CGCG 1 cut(s) 27
BstV1I GCAGC 8 cut(s) 62, 386, 389, 438, 488, 724, 796, 1051
BstV2I GAAGAC 1 cut(s) 1162
BstX2I RGATCY 1 cut(s) 625
BstXI CCANNNNNNTGG 1 cut(s) 1033
BstYI RGATCY 1 cut(s) 625
BsuI GTATCC 1 cut(s) 1115
BsuRI GGCC 1 cut(s) 1086
BtsCI GGATG 4 cut(s) 109, 613, 1302, 1443
BtsI GCAGTG 2 cut(s) 663, 777
BtsIMutI CAGTG 9 cut(s) 663, 777, 904, 1026, 1074, 1331, 1404, 1539, 1560
Cac8I GCNNGC 3 cut(s) 232, 369, 431
CaiI CAGNNNCTG 2 cut(s) 715, 1547
CciI TCATGA 1 cut(s) 309
CfoI GCGC 2 cut(s) 27, 382
Cfr13I GGNCC 1 cut(s) 524
Csp6I GTAC 4 cut(s) 211, 744, 959, 1395
CspCI CAANNNNNGTGG 2 cut(s) 1012, 1047
CviAII CATG 5 cut(s) 310, 850, 911, 920, 962
CviQI GTAC 4 cut(s) 211, 744, 959, 1395
DdeI CTNAG 7 cut(s) 171, 699, 980, 994, 1531, 1548, 1563
DpnI GATC 5 cut(s) 206, 345, 627, 929, 1489
DpnII GATC 5 cut(s) 204, 343, 625, 927, 1487
Eam1104I CTCTTC 2 cut(s) 816, 1202
EarI CTCTTC 2 cut(s) 816, 1202
Ecl136II GAGCTC 2 cut(s) 698, 883
Eco24I GRGCYC 2 cut(s) 700, 885
Eco47I GGWCC 1 cut(s) 524
Eco53kI GAGCTC 2 cut(s) 698, 883
Eco88I CYCGRG 1 cut(s) 142
EcoICRI GAGCTC 2 cut(s) 698, 883
EcoO109I RGGNCCY 1 cut(s) 524
EcoRII CCWGG 4 cut(s) 1081, 1101, 1117, 1129
EcoT22I ATGCAT 1 cut(s) 262
EcoT38I GRGCYC 2 cut(s) 700, 885
FaeI CATG 5 cut(s) 313, 853, 914, 923, 965
FalI AAGNNNNNCTT 2 cut(s) 497, 529
FaqI GGGAC 2 cut(s) 600, 1064
FatI CATG 5 cut(s) 309, 849, 910, 919, 961
FauNDI CATATG 1 cut(s) 573
FbaI TGATCA 2 cut(s) 204, 343
FokI GGATG 4 cut(s) 116, 620, 1309, 1450
FriOI GRGCYC 2 cut(s) 700, 885
FspBI CTAG 4 cut(s) 231, 243, 818, 1503
GlaI GCGC 2 cut(s) 26, 381
GsuI CTGGAG 1 cut(s) 1152
HaeIII GGCC 1 cut(s) 1086
HapII CCGG 2 cut(s) 685, 1158
HhaI GCGC 2 cut(s) 27, 382
Hin1II CATG 5 cut(s) 313, 853, 914, 923, 965
Hin6I GCGC 2 cut(s) 25, 380
HinP1I GCGC 2 cut(s) 25, 380
HincII GTYRAC 1 cut(s) 15
HindII GTYRAC 1 cut(s) 15
HinfI GANTC 3 cut(s) 136, 1184, 1544
HpaII CCGG 2 cut(s) 685, 1158
HphI GGTGA 3 cut(s) 158, 205, 773
Hpy166II GTNNAC 1 cut(s) 15
Hpy188I TCNGA 7 cut(s) 702, 997, 1227, 1352, 1485, 1543, 1549
Hpy188III TCNNGA 5 cut(s) 310, 514, 773, 818, 1570
Hpy8I GTNNAC 1 cut(s) 15
Hpy99I CGWCG 1 cut(s) 1352
HpyAV CCTTC 6 cut(s) 190, 295, 307, 547, 639, 682
HpyF10VI GCNNNNNNNGC 9 cut(s) 56, 368, 377, 461, 1055, 1064, 1145, 1154, 1328
HpyF3I CTNAG 7 cut(s) 171, 699, 980, 994, 1531, 1548, 1563
Hsp92II CATG 5 cut(s) 313, 853, 914, 923, 965
HspAI GCGC 2 cut(s) 25, 380
Ksp22I TGATCA 2 cut(s) 204, 343
Kzo9I GATC 5 cut(s) 204, 343, 625, 927, 1487
Lsp1109I GCAGC 8 cut(s) 62, 386, 389, 438, 488, 724, 796, 1051
LweI GCATC 5 cut(s) 22, 94, 243, 247, 1485
MaeI CTAG 4 cut(s) 231, 243, 818, 1503
MaeIII GTNAC 5 cut(s) 284, 386, 899, 1381, 1506
MalI GATC 5 cut(s) 206, 345, 627, 929, 1489
MboI GATC 5 cut(s) 204, 343, 625, 927, 1487
MboII GAAGA 8 cut(s) 41, 833, 1103, 1162, 1165, 1189, 1585, 1588
MflI RGATCY 1 cut(s) 625
MhlI GDGCHC 3 cut(s) 700, 885, 1502
MluCI AATT 7 cut(s) 64, 403, 531, 732, 1068, 1452, 1590
MlyI GAGTC 1 cut(s) 1538
MmeI TCCRAC 2 cut(s) 1330, 1357
MnlI CCTC 5 cut(s) 286, 907, 1052, 1242, 1450
Mph1103I ATGCAT 1 cut(s) 262
MroXI GAANNNNTTC 1 cut(s) 768
MseI TTAA 3 cut(s) 582, 750, 1005
MslI CAYNNNNRTG 4 cut(s) 144, 495, 996, 1031
MspA1I CMGCKG 1 cut(s) 715
MspI CCGG 2 cut(s) 685, 1158
MspR9I CCNGG 6 cut(s) 685, 1083, 1103, 1119, 1131, 1159
Mva1269I GAATGC 1 cut(s) 831
MvaI CCWGG 4 cut(s) 1083, 1103, 1119, 1131
MvnI CGCG 1 cut(s) 27
MwoI GCNNNNNNNGC 9 cut(s) 56, 368, 377, 461, 1055, 1064, 1145, 1154, 1328
NciI CCSGG 2 cut(s) 685, 1159
NdeI CATATG 1 cut(s) 573
NdeII GATC 5 cut(s) 204, 343, 625, 927, 1487
NheI GCTAGC 1 cut(s) 230
NlaIII CATG 5 cut(s) 313, 853, 914, 923, 965
NlaIV GGNNCC 1 cut(s) 627
NmuCI GTSAC 4 cut(s) 284, 386, 899, 1506
NsiI ATGCAT 1 cut(s) 262
OliI CACNNNNGTG 2 cut(s) 144, 1031
PagI TCATGA 1 cut(s) 309
PcsI WCGNNNNNNNCGW 1 cut(s) 946
PctI GAATGC 1 cut(s) 831
PdmI GAANNNNTTC 1 cut(s) 768
PfeI GAWTC 2 cut(s) 136, 1184
PfoI TCCNGGA 1 cut(s) 1129
PleI GAGTC 1 cut(s) 1538
PpsI GAGTC 1 cut(s) 1538
PpuMI RGGWCCY 1 cut(s) 524
PsiI TTATAA 2 cut(s) 726, 1280
Psp124BI GAGCTC 2 cut(s) 700, 885
Psp5II RGGWCCY 1 cut(s) 524
Psp6I CCWGG 4 cut(s) 1081, 1101, 1117, 1129
PspGI CCWGG 4 cut(s) 1081, 1101, 1117, 1129
PspN4I GGNNCC 1 cut(s) 627
PspPI GGNCC 1 cut(s) 524
PspPPI RGGWCCY 1 cut(s) 524
PsrI GAACNNNNNNTAC 4 cut(s) 288, 320, 1206, 1238
PstI CTGCAG 1 cut(s) 478
PstNI CAGNNNCTG 2 cut(s) 715, 1547
PsuI RGATCY 1 cut(s) 625
PvuII CAGCTG 1 cut(s) 715
RsaI GTAC 4 cut(s) 212, 745, 960, 1396
RsaNI GTAC 4 cut(s) 211, 744, 959, 1395
RseI CAYNNNNRTG 4 cut(s) 144, 495, 996, 1031
SacI GAGCTC 2 cut(s) 700, 885
SaqAI TTAA 3 cut(s) 582, 750, 1005
Sau3AI GATC 5 cut(s) 204, 343, 625, 927, 1487
Sau96I GGNCC 1 cut(s) 524
ScaI AGTACT 1 cut(s) 212
SchI GAGTC 1 cut(s) 1538
ScrFI CCNGG 6 cut(s) 685, 1083, 1103, 1119, 1131, 1159
SduI GDGCHC 3 cut(s) 700, 885, 1502
SfaNI GCATC 5 cut(s) 22, 94, 243, 247, 1485
SfcI CTRYAG 2 cut(s) 474, 1212
SinI GGWCC 1 cut(s) 524
SmiMI CAYNNNNRTG 4 cut(s) 144, 495, 996, 1031
SmlI CTYRAG 1 cut(s) 514
SmoI CTYRAG 1 cut(s) 514
Sse9I AATT 7 cut(s) 64, 403, 531, 732, 1068, 1452, 1590
SsiI CCGC 5 cut(s) 153, 167, 372, 462, 623
SspMI CTAG 4 cut(s) 231, 243, 818, 1503
SstI GAGCTC 2 cut(s) 700, 885
StyD4I CCNGG 6 cut(s) 683, 1081, 1101, 1117, 1129, 1157
TaqI TCGA 1 cut(s) 694
TasI AATT 7 cut(s) 64, 403, 531, 732, 1068, 1452, 1590
TatI WGTACW 2 cut(s) 210, 958
TauI GCSGC 2 cut(s) 170, 374
TfiI GAWTC 2 cut(s) 136, 1184
Tru1I TTAA 3 cut(s) 582, 750, 1005
Tru9I TTAA 3 cut(s) 582, 750, 1005
TscAI CASTG 9 cut(s) 670, 784, 904, 1033, 1081, 1338, 1404, 1539, 1567
TseFI GTSAC 4 cut(s) 284, 386, 899, 1506
TseI GCWGC 8 cut(s) 50, 374, 377, 426, 476, 712, 809, 1064
Tsp45I GTSAC 4 cut(s) 284, 386, 899, 1506
TspDTI ATGAA 8 cut(s) 132, 143, 326, 458, 866, 1153, 1165, 1189
TspRI CASTG 9 cut(s) 670, 784, 904, 1033, 1081, 1338, 1404, 1539, 1567
VpaK11BI GGWCC 1 cut(s) 524
XapI RAATTY 2 cut(s) 64, 403
XbaI TCTAGA 1 cut(s) 817
XcmI CCANNNNNNNNNTGG 1 cut(s) 1030
XmnI GAANNNNTTC 1 cut(s) 768
XspI CTAG 4 cut(s) 231, 243, 818, 1503
ZrmI AGTACT 1 cut(s) 212
Zsp2I ATGCAT 1 cut(s) 262
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.