pycom04g10720

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0004020
Physical Location & Seq
Reverse (-)
13465600 .. 13467735
2136 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g10720.1

Sequence Viewer

Length: 1671 bp
ATGGAATTGAATACAACGGAAGCTCCAGTAAGGCAAGAAAGCTTTGCCGGGAAGAAGATGACTAAGCAGTTGGCAGGAAAGCGCGACAACGGTCCCTTGCATTTGGCAGCTAGAGCAGGAGACTTGGGACTGGTTGTAGAAATCATGTCTAATTGTGGGGAGGAGGAGTTGAATGAACTGTCGTCGAAACAGAATTATGCGGGTGAAACTGCCTTGTATCTGGCGGCTGGTTGTGGATATGTTGGTTTGGTTAAGGAAATGATGAAGTACTATGATGTTGGTGCAGCTAGTATCAAAGCTGGGAACGGCTGTGATGCATTTCACGTTGCTTCCAAGCAAGGGCACTTAGAGGTACTGAAGATCCTCATGGAGGCGATTCCAGAACTTTCAATGACCGTTGATCGCGCAAACACCACGGCATTGCACATTGCTGCAGCACAGGGACACACTGAAGTAGTAAGTTTTCTTTTGGAGACTGGTTGCAGCTTGATGTCCATAGCAAGAAGCAATGGGAAAACTGCCTTGCATTCTGCAGCAAGGAAGGGGCATTTGGAGGTGGTCCAGGCCCTCTTGAGCAAAGATACCGGAACTGCAATAAGAAAAGATAGGAAGGGCCAGACAGCTCTCCATATGGCAGTCAAGGGACAGAATGTCGAATTGGTGGATGAGCTAGCGAAGGCAAATCCTTCATCGATAAACGAGGTAGATAACAAGGGAAACACTGCCTTGCACGTGGCAACCGGGAAGGGTCGAGCTCAGATTGTTCGAACGCTGTTAAGTTATAAGGGAGTGGCAAAAACCATCATTAACAGATCTGGAGAATCTGTTTTTGACACTGCTGAGAAATCTGGGCACTCAAAGATTGCCACCATTCTAGCAGAGCATGGGATTCAAAGTGCCAAATCCATGAAGCTGCCGACTTTGAACCCAAATCGAGAACTCAAACAAACTGTAAGTGAAATAAAACATGAGGTGCATGATCAGCTTAAGCAAACGAGACAAACAAGAAAGAAAGTGCATGAAATGGTAAAGCGGCTCGGCAAAATGCATTCGGAAGGGCTTAACAACGCAATAAACTCCACCACAGTTGTGGCTGTACTCACTGCCACTGTTGCCTTTGCTGCAATCTTCAGTGTCCCGGGCCAATACCCTGACCCTAAAAAACCTCTTCCTGATGGATTCTCTCCCGGAGAAGCAAACATTGCCCCCAAAACTGAGTTCATAGTCTTCTTCATCTTTGACTCATTTGCTCTCTTCATATCATTGGGTGTTGTGGTGGTTCAAACTTCTATTGTTGTCGTAGAGAGGGAGGCAAAAGAGAGGCTAATGGCAGTCATAAACAAGCTAATGTGGATGGCGTGTGTGATGGTTTCAGTTGCATTTCTTGCACTATCATATGTAATTGTTGGAAAAGAAGAAAAATGGCTGGCTGTTGGAGTGACAGCTATAGGGACAGTGATCATGGGAATGACACTAGGAACAATGTGTTATTGGATGGTTGTGCAGCGCGTTGAAGCATCTAAATATCGTTGTCACATTCGGAGATCATCGCTGAGCATCCACTCACACTCACACTCAAACTCAAACTCACACTCTTGCTCTTTCTCAGTGATATCGGATTCGGAGCTTCTGAACACCGAGTTTAAGAGTAAGAAAGTTTATGCAATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005886 GO:0012505 GO:0016020 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 Ank_3 Ank_4 PGG

Protein Analysis

557

Amino Acids

60.29

Weight (kDa)

9.13

Isoelectric Point (pI)

30.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 33 - 124 8.2e-10 Ankyrin repeats (3 copies)
Ank_2 PF12796 99 - 163 4.7e-11 Ankyrin repeats (3 copies)
Ank_2 PF12796 107 - 194 5.1e-19 Ankyrin repeats (3 copies)
Ank_4 PF13637 108 - 157 3.5e-09 Ankyrin repeats (many copies)
Ank_2 PF12796 169 - 227 2.9e-12 Ankyrin repeats (3 copies)
Ank PF00023 170 - 192 7.5e-06 Ankyrin repeat
Ank_4 PF13637 175 - 221 1.4e-07 Ankyrin repeats (many copies)
Ank_5 PF13857 200 - 246 2.1e-08 Ankyrin repeats (many copies)
Ank_2 PF12796 209 - 298 3.1e-12 Ankyrin repeats (3 copies)
Ank_4 PF13637 209 - 259 4.8e-07 Ankyrin repeats (many copies)
PGG PF13962 352 - 467 2.3e-27 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 783
AccII CGCG 3 cut(s) 84, 405, 1509
AciI CCGC 3 cut(s) 200, 224, 1033
AclWI GGATC 1 cut(s) 355
AcuI CTGAAG 3 cut(s) 377, 471, 1114
AcvI CACGTG 1 cut(s) 733
AfaI GTAC 3 cut(s) 269, 354, 1098
AfiI CCNNNNNNNGG 2 cut(s) 339, 370
AflII CTTAAG 1 cut(s) 986
AgsI TTSAA 7 cut(s) 10, 172, 390, 893, 925, 1283, 1514
AhdI GACNNNNNGTC 1 cut(s) 650
AjnI CCWGG 1 cut(s) 561
AjuI GAANNNNNNNTTGG 6 cut(s) 533, 565, 641, 673, 1202, 1234
AleI CACNNNNGTG 1 cut(s) 1088
Alw21I GWGCWC 1 cut(s) 757
Alw26I GTCTC 3 cut(s) 114, 467, 991
AlwI GGATC 1 cut(s) 355
Ama87I CYCGRG 1 cut(s) 1138
AoxI GGCC 3 cut(s) 564, 613, 1141
ApeKI GCWGC 9 cut(s) 107, 284, 431, 434, 483, 533, 913, 1121, 1504
AspLEI GCGC 3 cut(s) 84, 407, 1509
AspS9I GGNCC 5 cut(s) 92, 559, 565, 613, 1141
AsuC2I CCSGG 5 cut(s) 49, 742, 1139, 1140, 1188
AsuHPI GGTGA 1 cut(s) 215
AsuII TTCGAA 1 cut(s) 766
AsuNHI GCTAGC 1 cut(s) 670
AvaI CYCGRG 1 cut(s) 1138
AvaII GGWCC 2 cut(s) 92, 559
BaeGI GKGCMC 2 cut(s) 345, 855
BanII GRGCYC 1 cut(s) 757
BbrPI CACGTG 1 cut(s) 733
BbsI GAAGAC 1 cut(s) 1219
Bbv12I GWGCWC 1 cut(s) 757
BbvI GCAGC 9 cut(s) 119, 296, 418, 446, 495, 545, 900, 1108, 1516
BccI CCATC 5 cut(s) 809, 1169, 1348, 1360, 1489
BceAI ACGGC 2 cut(s) 322, 432
BciT130I CCWGG 1 cut(s) 563
BclI TGATCA 2 cut(s) 979, 1458
BcnI CCSGG 5 cut(s) 49, 742, 1139, 1140, 1188
BcoDI GTCTC 3 cut(s) 114, 467, 991
BfaI CTAG 5 cut(s) 111, 288, 671, 875, 1475
BfmI CTRYAG 3 cut(s) 432, 531, 1446
BfrI CTTAAG 1 cut(s) 986
BglII AGATCT 1 cut(s) 812
BlpI GCTNAGC 1 cut(s) 1553
BmcAI AGTACT 1 cut(s) 269
Bme1390I CCNGG 6 cut(s) 49, 563, 742, 1139, 1140, 1188
Bme18I GGWCC 2 cut(s) 92, 559
BmeRI GACNNNNNGTC 1 cut(s) 650
BmeT110I CYCGRG 1 cut(s) 1138
BmgT120I GGNCC 5 cut(s) 92, 559, 565, 613, 1141
BmiI GGNNCC 1 cut(s) 94
BmrFI CCNGG 6 cut(s) 49, 563, 742, 1139, 1140, 1188
BmsI GCATC 3 cut(s) 304, 1526, 1566
BmtI GCTAGC 1 cut(s) 674
BoxI GACNNNNGTC 1 cut(s) 90
BpiI GAAGAC 1 cut(s) 1219
BpmI CTGGAG 2 cut(s) 9, 837
Bpu1102I GCTNAGC 1 cut(s) 1553
Bpu14I TTCGAA 1 cut(s) 766
BpuEI CTTGAG 1 cut(s) 592
BpuMI CCSGG 5 cut(s) 49, 742, 1139, 1140, 1188
Bsa29I ATCGAT 1 cut(s) 692
BsaAI YACGTR 1 cut(s) 733
BsaJI CCNNGG 2 cut(s) 414, 1138
BsaWI WCCGGW 1 cut(s) 584
BsaXI ACNNNNNCTCC 6 cut(s) 7, 37, 152, 182, 810, 840
Bsc4I CCNNNNNNNGG 2 cut(s) 339, 370
Bse1I ACTGG 3 cut(s) 26, 135, 481
Bse3DI GCAATG 4 cut(s) 419, 426, 514, 1200
BseBI CCWGG 1 cut(s) 563
BseCI ATCGAT 1 cut(s) 692
BseDI CCNNGG 2 cut(s) 414, 1138
BseGI GGATG 4 cut(s) 670, 1359, 1500, 1557
BseLI CCNNNNNNNGG 2 cut(s) 339, 370
BseMI GCAATG 4 cut(s) 419, 426, 514, 1200
BseMII CTCAG 5 cut(s) 770, 831, 1206, 1544, 1620
BseNI ACTGG 3 cut(s) 26, 135, 481
BseRI GAGGAG 2 cut(s) 176, 179
BseSI GKGCMC 2 cut(s) 345, 855
BseXI GCAGC 9 cut(s) 119, 296, 418, 446, 495, 545, 900, 1108, 1516
BseYI CCCAGC 1 cut(s) 299
BsgI GTGCAG 2 cut(s) 303, 1523
Bsh1236I CGCG 3 cut(s) 84, 405, 1509
BshFI GGCC 3 cut(s) 566, 615, 1143
BshVI ATCGAT 1 cut(s) 692
BsiHKAI GWGCWC 1 cut(s) 757
BsiHKCI CYCGRG 1 cut(s) 1138
BsiSI CCGG 5 cut(s) 48, 585, 741, 1139, 1188
BslFI GGGAC 6 cut(s) 78, 141, 456, 657, 1121, 1465
BslI CCNNNNNNNGG 2 cut(s) 339, 370
BsmAI GTCTC 3 cut(s) 114, 467, 991
BsmFI GGGAC 6 cut(s) 78, 141, 456, 657, 1121, 1465
BsmI GAATGC 2 cut(s) 526, 1048
BsnI GGCC 3 cut(s) 566, 615, 1143
BsoBI CYCGRG 1 cut(s) 1138
Bsp119I TTCGAA 1 cut(s) 766
Bsp1286I GDGCHC 3 cut(s) 345, 757, 855
Bsp143I GATC 6 cut(s) 360, 400, 812, 979, 1458, 1544
Bsp1720I GCTNAGC 1 cut(s) 1553
BspACI CCGC 3 cut(s) 200, 224, 1033
BspANI GGCC 3 cut(s) 566, 615, 1143
BspCNI CTCAG 5 cut(s) 769, 832, 1207, 1545, 1619
BspDI ATCGAT 1 cut(s) 692
BspFNI CGCG 3 cut(s) 84, 405, 1509
BspLI GGNNCC 1 cut(s) 94
BspMAI CTGCAG 2 cut(s) 436, 535
BspOI GCTAGC 1 cut(s) 674
BspPI GGATC 1 cut(s) 355
BspT104I TTCGAA 1 cut(s) 766
BspTI CTTAAG 1 cut(s) 986
BsrDI GCAATG 4 cut(s) 419, 426, 514, 1200
BsrI ACTGG 3 cut(s) 26, 135, 481
BssECI CCNNGG 2 cut(s) 414, 1138
BssMI GATC 6 cut(s) 360, 400, 812, 979, 1458, 1544
Bst2UI CCWGG 1 cut(s) 563
Bst4CI ACNGT 7 cut(s) 92, 180, 397, 952, 1087, 1111, 1456
Bst6I CTCTTC 2 cut(s) 1173, 1259
BstAFI CTTAAG 1 cut(s) 986
BstAPI GCANNNNNTGC 2 cut(s) 1202, 1385
BstBAI YACGTR 1 cut(s) 733
BstBI TTCGAA 1 cut(s) 766
BstC8I GCNNGC 2 cut(s) 672, 1428
BstDEI CTNAG 7 cut(s) 63, 346, 756, 840, 1215, 1553, 1606
BstDSI CCRYGG 1 cut(s) 414
BstENI CCTNNNNNAGG 1 cut(s) 368
BstF5I GGATG 4 cut(s) 670, 1359, 1500, 1557
BstFNI CGCG 3 cut(s) 84, 405, 1509
BstHHI GCGC 3 cut(s) 84, 407, 1509
BstKTI GATC 6 cut(s) 363, 403, 815, 982, 1461, 1547
BstMAI GTCTC 3 cut(s) 114, 467, 991
BstMBI GATC 6 cut(s) 360, 400, 812, 979, 1458, 1544
BstMWI GCNNNNNNNGC 6 cut(s) 113, 982, 1112, 1121, 1202, 1385
BstNI CCWGG 1 cut(s) 563
BstPAI GACNNNNGTC 1 cut(s) 90
BstSCI CCNGG 6 cut(s) 47, 561, 740, 1137, 1138, 1186
BstSFI CTRYAG 3 cut(s) 432, 531, 1446
BstSLI GKGCMC 2 cut(s) 345, 855
BstUI CGCG 3 cut(s) 84, 405, 1509
BstV1I GCAGC 9 cut(s) 119, 296, 418, 446, 495, 545, 900, 1108, 1516
BstV2I GAAGAC 1 cut(s) 1219
BstX2I RGATCY 2 cut(s) 360, 812
BstXI CCANNNNNNTGG 1 cut(s) 1090
BstYI RGATCY 2 cut(s) 360, 812
Bsu15I ATCGAT 1 cut(s) 692
BsuRI GGCC 3 cut(s) 566, 615, 1143
BsuTUI ATCGAT 1 cut(s) 692
BtgI CCRYGG 1 cut(s) 414
BtgZI GCGATG 1 cut(s) 1533
BtsCI GGATG 4 cut(s) 670, 1359, 1500, 1557
BtsI GCAGTG 3 cut(s) 720, 834, 1101
BtsIMutI CAGTG 8 cut(s) 447, 720, 834, 1101, 1107, 1138, 1461, 1614
Cac8I GCNNGC 2 cut(s) 672, 1428
CfoI GCGC 3 cut(s) 84, 407, 1509
Cfr13I GGNCC 5 cut(s) 92, 559, 565, 613, 1141
Cfr9I CCCGGG 1 cut(s) 1138
ClaI ATCGAT 1 cut(s) 692
Csp6I GTAC 3 cut(s) 268, 353, 1097
CspCI CAANNNNNGTGG 2 cut(s) 1069, 1104
CviAII CATG 8 cut(s) 145, 367, 884, 907, 968, 977, 1019, 1462
CviQI GTAC 3 cut(s) 268, 353, 1097
DdeI CTNAG 7 cut(s) 63, 346, 756, 840, 1215, 1553, 1606
DpnI GATC 6 cut(s) 362, 402, 814, 981, 1460, 1546
DpnII GATC 6 cut(s) 360, 400, 812, 979, 1458, 1544
DriI GACNNNNNGTC 1 cut(s) 650
Eam1104I CTCTTC 2 cut(s) 1173, 1259
Eam1105I GACNNNNNGTC 1 cut(s) 650
EarI CTCTTC 2 cut(s) 1173, 1259
Ecl136II GAGCTC 1 cut(s) 755
Eco24I GRGCYC 1 cut(s) 757
Eco32I GATATC 1 cut(s) 1614
Eco47I GGWCC 2 cut(s) 92, 559
Eco53kI GAGCTC 1 cut(s) 755
Eco57I CTGAAG 3 cut(s) 377, 471, 1114
Eco72I CACGTG 1 cut(s) 733
Eco88I CYCGRG 1 cut(s) 1138
EcoICRI GAGCTC 1 cut(s) 755
EcoNI CCTNNNNNAGG 1 cut(s) 368
EcoO109I RGGNCCY 1 cut(s) 565
EcoRII CCWGG 1 cut(s) 561
EcoRV GATATC 1 cut(s) 1614
EcoT22I ATGCAT 2 cut(s) 319, 1050
EcoT38I GRGCYC 1 cut(s) 757
FaeI CATG 8 cut(s) 148, 370, 887, 910, 971, 980, 1022, 1465
FaqI GGGAC 6 cut(s) 78, 141, 456, 657, 1121, 1465
FatI CATG 8 cut(s) 144, 366, 883, 906, 967, 976, 1018, 1461
FauI CCCGC 1 cut(s) 193
FauNDI CATATG 2 cut(s) 630, 1396
FbaI TGATCA 2 cut(s) 979, 1458
FokI GGATG 4 cut(s) 677, 1366, 1507, 1544
FriOI GRGCYC 1 cut(s) 757
FspBI CTAG 5 cut(s) 111, 288, 671, 875, 1475
GlaI GCGC 3 cut(s) 83, 406, 1508
GsaI CCCAGC 1 cut(s) 303
GsuI CTGGAG 2 cut(s) 9, 837
HaeIII GGCC 3 cut(s) 566, 615, 1143
HapII CCGG 5 cut(s) 48, 585, 741, 1139, 1188
HhaI GCGC 3 cut(s) 84, 407, 1509
Hin1II CATG 8 cut(s) 148, 370, 887, 910, 971, 980, 1022, 1465
Hin6I GCGC 3 cut(s) 82, 405, 1507
HinP1I GCGC 3 cut(s) 82, 405, 1507
HindIII AAGCTT 1 cut(s) 40
HinfI GANTC 6 cut(s) 376, 821, 889, 1179, 1241, 1619
HpaII CCGG 5 cut(s) 48, 585, 741, 1139, 1188
HphI GGTGA 1 cut(s) 215
Hpy188I TCNGA 6 cut(s) 759, 1054, 1542, 1618, 1624, 1632
Hpy188III TCNNGA 5 cut(s) 380, 571, 816, 935, 1172
Hpy99I CGWCG 1 cut(s) 187
HpyAV CCTTC 6 cut(s) 535, 604, 670, 696, 739, 1049
HpyCH4III ACNGT 7 cut(s) 92, 180, 397, 952, 1087, 1111, 1456
HpyCH4IV ACGT 2 cut(s) 324, 732
HpyF10VI GCNNNNNNNGC 6 cut(s) 113, 982, 1112, 1121, 1202, 1385
HpyF3I CTNAG 7 cut(s) 63, 346, 756, 840, 1215, 1553, 1606
HpySE526I ACGT 2 cut(s) 324, 732
Hsp92II CATG 8 cut(s) 148, 370, 887, 910, 971, 980, 1022, 1465
HspAI GCGC 3 cut(s) 82, 405, 1507
Ksp22I TGATCA 2 cut(s) 979, 1458
Kzo9I GATC 6 cut(s) 360, 400, 812, 979, 1458, 1544
LmnI GCTCC 2 cut(s) 28, 1624
Lsp1109I GCAGC 9 cut(s) 119, 296, 418, 446, 495, 545, 900, 1108, 1516
LweI GCATC 3 cut(s) 304, 1526, 1566
MaeI CTAG 5 cut(s) 111, 288, 671, 875, 1475
MaeII ACGT 2 cut(s) 324, 732
MaeIII GTNAC 2 cut(s) 1438, 1532
MalI GATC 6 cut(s) 362, 402, 814, 981, 1460, 1546
MboI GATC 6 cut(s) 360, 400, 812, 979, 1458, 1544
MboII GAAGA 9 cut(s) 64, 67, 370, 1120, 1160, 1219, 1222, 1246, 1427
MflI RGATCY 2 cut(s) 360, 812
MhlI GDGCHC 3 cut(s) 345, 757, 855
MluCI AATT 6 cut(s) 5, 151, 193, 656, 1401, 1665
MlyI GAGTC 1 cut(s) 1235
MmeI TCCRAC 2 cut(s) 1387, 1414
Mph1103I ATGCAT 2 cut(s) 319, 1050
MseI TTAA 6 cut(s) 252, 776, 807, 987, 1062, 1644
MslI CAYNNNNRTG 2 cut(s) 1088, 1466
MspCI CTTAAG 1 cut(s) 986
MspI CCGG 5 cut(s) 48, 585, 741, 1139, 1188
MspR9I CCNGG 6 cut(s) 49, 563, 742, 1139, 1140, 1188
Mva1269I GAATGC 2 cut(s) 526, 1048
MvaI CCWGG 1 cut(s) 563
MvnI CGCG 3 cut(s) 84, 405, 1509
MwoI GCNNNNNNNGC 6 cut(s) 113, 982, 1112, 1121, 1202, 1385
NciI CCSGG 5 cut(s) 49, 742, 1139, 1140, 1188
NdeI CATATG 2 cut(s) 630, 1396
NdeII GATC 6 cut(s) 360, 400, 812, 979, 1458, 1544
NheI GCTAGC 1 cut(s) 670
NlaIII CATG 8 cut(s) 148, 370, 887, 910, 971, 980, 1022, 1465
NlaIV GGNNCC 1 cut(s) 94
NmeAIII GCCGAG 1 cut(s) 1017
NmuCI GTSAC 2 cut(s) 1438, 1532
NsiI ATGCAT 2 cut(s) 319, 1050
NspV TTCGAA 1 cut(s) 766
OliI CACNNNNGTG 1 cut(s) 1088
PctI GAATGC 2 cut(s) 526, 1048
PfeI GAWTC 5 cut(s) 376, 821, 889, 1179, 1619
PfoI TCCNGGA 1 cut(s) 1186
PleI GAGTC 1 cut(s) 1235
PmaCI CACGTG 1 cut(s) 733
PmlI CACGTG 1 cut(s) 733
PpsI GAGTC 1 cut(s) 1235
Ppu21I YACGTR 1 cut(s) 733
PshAI GACNNNNGTC 1 cut(s) 90
PsiI TTATAA 1 cut(s) 783
Psp124BI GAGCTC 1 cut(s) 757
Psp6I CCWGG 1 cut(s) 561
PspCI CACGTG 1 cut(s) 733
PspFI CCCAGC 1 cut(s) 299
PspGI CCWGG 1 cut(s) 561
PspN4I GGNNCC 1 cut(s) 94
PspPI GGNCC 5 cut(s) 92, 559, 565, 613, 1141
PstI CTGCAG 2 cut(s) 436, 535
PsuI RGATCY 2 cut(s) 360, 812
RsaI GTAC 3 cut(s) 269, 354, 1098
RsaNI GTAC 3 cut(s) 268, 353, 1097
RseI CAYNNNNRTG 2 cut(s) 1088, 1466
SacI GAGCTC 1 cut(s) 757
SaqAI TTAA 6 cut(s) 252, 776, 807, 987, 1062, 1644
Sau3AI GATC 6 cut(s) 360, 400, 812, 979, 1458, 1544
Sau96I GGNCC 5 cut(s) 92, 559, 565, 613, 1141
ScaI AGTACT 1 cut(s) 269
SchI GAGTC 1 cut(s) 1235
ScrFI CCNGG 6 cut(s) 49, 563, 742, 1139, 1140, 1188
SduI GDGCHC 3 cut(s) 345, 757, 855
SfaNI GCATC 3 cut(s) 304, 1526, 1566
SfcI CTRYAG 3 cut(s) 432, 531, 1446
SfuI TTCGAA 1 cut(s) 766
SinI GGWCC 2 cut(s) 92, 559
SmaI CCCGGG 1 cut(s) 1140
SmiMI CAYNNNNRTG 2 cut(s) 1088, 1466
SmlI CTYRAG 2 cut(s) 571, 986
SmoI CTYRAG 2 cut(s) 571, 986
Sse9I AATT 6 cut(s) 5, 151, 193, 656, 1401, 1665
SsiI CCGC 3 cut(s) 200, 224, 1033
SspMI CTAG 5 cut(s) 111, 288, 671, 875, 1475
SstI GAGCTC 1 cut(s) 757
StyD4I CCNGG 6 cut(s) 47, 561, 740, 1137, 1138, 1186
TaaI ACNGT 7 cut(s) 92, 180, 397, 952, 1087, 1111, 1456
TaiI ACGT 2 cut(s) 327, 735
TaqI TCGA 6 cut(s) 185, 654, 692, 751, 766, 934
TasI AATT 6 cut(s) 5, 151, 193, 656, 1401, 1665
TatI WGTACW 2 cut(s) 267, 1096
TauI GCSGC 2 cut(s) 227, 1036
TfiI GAWTC 5 cut(s) 376, 821, 889, 1179, 1619
Tru1I TTAA 6 cut(s) 252, 776, 807, 987, 1062, 1644
Tru9I TTAA 6 cut(s) 252, 776, 807, 987, 1062, 1644
TscAI CASTG 8 cut(s) 454, 727, 841, 1108, 1114, 1138, 1461, 1614
TseFI GTSAC 2 cut(s) 1438, 1532
TseI GCWGC 9 cut(s) 107, 284, 431, 434, 483, 533, 913, 1121, 1504
Tsp45I GTSAC 2 cut(s) 1438, 1532
TspDTI ATGAA 8 cut(s) 189, 278, 678, 923, 1035, 1210, 1222, 1246
TspGWI ACGGA 1 cut(s) 32
TspMI CCCGGG 1 cut(s) 1138
TspRI CASTG 8 cut(s) 454, 727, 841, 1108, 1114, 1138, 1461, 1614
Vha464I CTTAAG 1 cut(s) 986
VpaK11BI GGWCC 2 cut(s) 92, 559
XagI CCTNNNNNAGG 1 cut(s) 368
XcmI CCANNNNNNNNNTGG 1 cut(s) 1087
XmaI CCCGGG 1 cut(s) 1138
XspI CTAG 5 cut(s) 111, 288, 671, 875, 1475
ZrmI AGTACT 1 cut(s) 269
Zsp2I ATGCAT 2 cut(s) 319, 1050
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.