Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb6

Orthogroup ID

OG0013292

Physical Location & Seq

Genomic Coordinates Reverse (-)

9521790 .. 9529658

Gene Structure 7869 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_6g15280.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2274 bp

ATGTACGGCGCGGCGGGGAAGAGCACGAGCTCGAGCTACGGCGATTTTGGGCTCGCCGAGTTATGGGTCGCGTACTTGGCCCAGATCGAGCTCGGCGACGACAAACCCTGGCTCAACCTCACTAAATCACTCATCAGCTTCTTCAAAACGGCGCCGGGAGAAGCTCTTCTTTCTCAGGTGAAGGACGAGGACGGCGTCGTTGTGCTGTGGGTTGACTTTGAGAAGTTTCGGAAAGAGTGTGGTGTTGAGGAGTTTTGTGAAGCGTTGGAGGAGAAGCCGAAGGTGACGCTGTTGTGCATGAGTATTGCAGTGCACAAGGTTCTGTTGAGTCATTGGGAAAGTAACAGAATGGATGATGATTTCCGAGTTAATATCCGGTTGCATAACCATCCGAAATCGATGATCGCTTTGAAGAACTTAAAAGCTGCATATATAGACAAGCTTGTATCTGTGCGCGGTAGTGTAGTCAAAGTTAGCACTGTGAGGCCTTTTGTGGTGCAAATGGATTTTGACTGTGGGAAGTGTAAGACGAGCATTACGCGTATGTTTCCTGATGGGAAGTTTTCGCCACCGTTGAAATGTGATCTACATGCATGCAAGAGCAGAACTTTTGTTCCAGATCGATCTACTGCTCAAACGATAGATTTTCAGAAGATAAGGATACAGGAGCTGCTAAAGCCTGAAGATCATGAAGAAGGCAGGGTGCCTCGAACAGTAGAATGTGAACTGCTTGAAGATCTTGTTGATGCATGCATCCCTGGAGATGTGGTGACTGTCACTGGGATAATAAGAGTCATTAACAATTACATGGACATTGGGGGAGGAAAATCAAAAGGCAAAAGTCAGGGAATATACTATCTGTATCTAGAAGCTGTTTCAATTAAAAACTCTAAGTCACAGTCTATGCCTGAGGGTGCACAAGATTCTAGTTCTAATGCTGGGTCGTTAGCACTTGTTGACTCATTTTCCCCTAGGGATTTGGAATTCATTGTCAAGTTCTCGGAGGAACATGGTCCAGATACCTTCCGGCAACTTCTCCAATCTGTTTGTCCATCCATCTATGGTCATGAGCTTGTTAAAGCGGGGATAATACTGGCACTCTTTGGAGGTGTAAGGAAGCACTCAAATGATCAAAACAAGGTTCCTGTTAGAGGAGACATTCATGTCATTATTGTTGGTGATCCTGGACTTGGAAAGAGCCAAATACTTCAAGCAGCAGCAGCTGTTTCTCCGCGAGGCATTTATGTATGTGGTAATGCCACAACCAGAGCCGGACTCACTGTAGCTGTTGTAAAGGATTCTATGACAAGTGATTATGCATTCGAAGCAGGTGCCATGGTACTTGCAGACAGTGGCTTGTGTTGTATTGACGAATTTGACAAAATGACTTCAGAGCATCAGGCCCTACTAGAAGCCATGGAACAACAGTGTGTGTCTATTGCAAAGGCTGGATTAGTGGCAAGTCTGTCAGCACGAACCTCTGTCTTGGCAGCAGCAAACCCTGTTGGTGGTCATTATAATCGTGCCAAAACCGTGAATGAAAATCTGAAAATAGGTGCAGCTCTCCTCTCACGATTTGATTTGGTTTTCATATTACTTGACAAACCTGATGAACTTCTGGATAAGCGAGTTTCAGAGCACATTATGTCACTTCATACTGGATATGGAGAGCACTCACCAGCTGCAAAAAAGATTAGTAGAGAAAATGCAGCATCACAAAGTGCAGAAGACATAGATATGAGTGGAGGTCGTTCTTTGGTTTCAAGGTTGAAGCTTGACCCAAAGAAGGATGCTGATTTCGTTCCATTACCGATGCAACTTCTCCGCAAGTACATTGCTTATGCAAGGACTTTTGTCTTTCCAAGGATGTCAAAGCCGGCAGCAGAAATCCTGCAGAAATTTTACTTGAAACTGAGAGACCATGCTACCTCCGCTGATAGCACACCAATAACAGCGAGACAACTGGAGAGTCTGGTGAGGCTGACAGAAGCTCGAGCTAGGTTGGATTTAAGGGAGGAAATTACTGCCCAAGATGCAATGGATGTAGTTGAAATCATGAGAGAATCCTTGTATGACAAGTACATTGATGAGCATGGTGTTGTGGATTTTGGTCGCAGTGGAGGGATGAGTCAACAAAAAGAAGCAAAGCGGTTTTTAAGTGCCCTTAATAAGCAATCAGAGTTGCAGCAGAAAGACACTTTCACTATTTCTTGTTGGCTATCTACTCAAAAAAGGGGCGAAGACATACCAGGTACTATCATCATCTTATTCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000280 GO:0000724 GO:0000725 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006259 GO:0006281 GO:0006302 GO:0006310 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007049 GO:0007140 GO:0007143 GO:0007275 GO:0007276 GO:0007292 GO:0008150 GO:0008152 GO:0009555 GO:0009987 GO:0016043 GO:0019953 GO:0022402 GO:0022412 GO:0022414 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032504 GO:0032991 GO:0033554 GO:0034641 GO:0042555 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0044703 GO:0046483 GO:0048229 GO:0048232 GO:0048285 GO:0048609 GO:0048856 GO:0050896 GO:0051321 GO:0051704 GO:0051716 GO:0070013 GO:0071704 GO:0071840 GO:0090304 GO:0097362 GO:0140013 GO:1901360 GO:1903046

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MCM MCM_OB

Protein Analysis

758

Amino Acids

83.58

Weight (kDa)

6.32

Isoelectric Point (pI)

43.74

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MCM_OB	PF17207	137 - 266	9.7e-29	MCM OB domain
MCM	PF00493	338 - 552	2.4e-89	MCM P-loop domain
AAA_5	PF07728	388 - 507	4.7e-07	AAA domain (dynein-related subfamily)
MCM_lid	PF17855	607 - 690	5.6e-26	MCM AAA-lid domain
WHD_MCM8	PF25051	702 - 737	5.5e-10	MCM8 winged helix domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0013292)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G09660 AT3G09660 AT3G09660
fragaria_vesca	FvH4_6g15280 FvH4_6g15280
malus_domestica	MD04G1104500.v1.1
prunus_persica	Prupe.6G241500_v2.0.a1 Prupe.6G241500_v2.0.a1
pyrus_communis	pycom04g09950
rosa_chinensis	RchiOBHm_Chr3g0468711
rosa_laevigata	RLG00000024414
rosa_multiflora	Rmu_sc0011538.1_g000001
rosa_roxburghii	Rroxscaffold_6G00412640
rosa_rugosa	Rorug03G0096200
rosa_samantha	Rh3AG145200 Rh3BG167800 Rh3DG166500
rosa_wichuraiana	Rw3G013700

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1518
AarI	CACCTGC	1 cut(s)	1319
AasI	GACNNNNNNGTC	1 cut(s)	1163
Acc36I	ACCTGC	1 cut(s)	1319
AccB1I	GGYRCC	3 cut(s)	151, 703, 1331
AccII	CGCG	5 cut(s)	11, 71, 456, 541, 1234
AciI	CCGC	8 cut(s)	11, 14, 456, 1082, 1232, 1825, 1932, 2149
AclWI	GGATC	1 cut(s)	1175
AcsI	RAATTY	3 cut(s)	983, 1373, 1898
AcuI	CTGAAG	2 cut(s)	702, 1374
AcyI	GRCGYC	2 cut(s)	152, 195
AdeI	CACNNNGTG	1 cut(s)	1721
AfaI	GTAC	6 cut(s)	5, 74, 1341, 1832, 2081, 2254
AfiI	CCNNNNNNNGG	5 cut(s)	63, 1151, 1190, 1508, 1786
AflIII	ACRYGT	1 cut(s)	539
AjnI	CCWGG	4 cut(s)	107, 757, 1183, 2248
AloI	GAACNNNNNNTCC	2 cut(s)	598, 630
Alw21I	GWGCWC	7 cut(s)	26, 32, 93, 315, 919, 1641, 1674
Alw26I	GTCTC	3 cut(s)	1149, 1911, 1951
Alw44I	GTGCAC	2 cut(s)	311, 915
AlwI	GGATC	1 cut(s)	1175
AlwNI	CAGNNNCTG	2 cut(s)	670, 1223
Ama87I	CYCGRG	2 cut(s)	31, 1992
AoxI	GGCC	3 cut(s)	78, 485, 1401
ApaLI	GTGCAC	2 cut(s)	311, 915
ApoI	RAATTY	3 cut(s)	983, 1373, 1898
Asp700I	GAANNNNTTC	1 cut(s)	165
AspA2I	CCTAGG	1 cut(s)	971
AspLEI	GCGC	3 cut(s)	11, 154, 456
AspS9I	GGNCC	3 cut(s)	79, 1013, 1402
AsuC2I	CCSGG	1 cut(s)	156
AsuHPI	GGTGA	6 cut(s)	190, 295, 781, 1190, 1668, 1987
AsuII	TTCGAA	1 cut(s)	1323
AvaI	CYCGRG	2 cut(s)	31, 1992
AvaII	GGWCC	1 cut(s)	1013
AvrII	CCTAGG	1 cut(s)	971
AxyI	CCTNAGG	1 cut(s)	909
BaeGI	GKGCMC	3 cut(s)	315, 919, 2164
BanI	GGYRCC	3 cut(s)	151, 703, 1331
BanII	GRGCYC	3 cut(s)	32, 54, 93
BauI	CACGAG	1 cut(s)	25
BbsI	GAAGAC	2 cut(s)	1734, 2247
Bbv12I	GWGCWC	7 cut(s)	26, 32, 93, 315, 919, 1641, 1674
BccI	CCATC	4 cut(s)	396, 548, 1060, 1064
BceAI	ACGGC	4 cut(s)	22, 55, 165, 208
BcgI	CGANNNNNNTGC	2 cut(s)	1313, 1347
BciT130I	CCWGG	4 cut(s)	109, 759, 1185, 2250
BciVI	GTATCC	1 cut(s)	654
BclI	TGATCA	1 cut(s)	1129
BcnI	CCSGG	1 cut(s)	156
BcoDI	GTCTC	3 cut(s)	1149, 1911, 1951
BfaI	CTAG	6 cut(s)	866, 927, 972, 1409, 1998, 2272
BfmI	CTRYAG	2 cut(s)	1281, 1892
BfoI	RGCGCY	1 cut(s)	155
BfuAI	ACCTGC	1 cut(s)	1319
BfuI	GTATCC	1 cut(s)	654
BglII	AGATCT	1 cut(s)	736
BlnI	CCTAGG	1 cut(s)	971
Bme1390I	CCNGG	5 cut(s)	109, 156, 759, 1185, 2250
Bme18I	GGWCC	1 cut(s)	1013
BmeT110I	CYCGRG	2 cut(s)	31, 1992
BmgT120I	GGNCC	3 cut(s)	79, 1013, 1402
BmiI	GGNNCC	4 cut(s)	153, 705, 1143, 1333
BmrFI	CCNGG	5 cut(s)	109, 156, 759, 1185, 2250
BmrI	ACTGGG	1 cut(s)	789
BmsI	GCATC	7 cut(s)	736, 762, 1405, 1721, 1780, 1803, 2023
BmuI	ACTGGG	1 cut(s)	789
BoxI	GACNNNNGTC	1 cut(s)	1853
BpiI	GAAGAC	2 cut(s)	1734, 2247
BplI	GAGNNNNNCTC	2 cut(s)	1260, 1292
BpmI	CTGGAG	2 cut(s)	780, 1985
Bpu14I	TTCGAA	1 cut(s)	1323
BpuMI	CCSGG	1 cut(s)	156
Bsa29I	ATCGAT	2 cut(s)	398, 622
BsaHI	GRCGYC	2 cut(s)	152, 195
BsaI	GGTCTC	1 cut(s)	1911
BsaJI	CCNNGG	6 cut(s)	107, 757, 971, 1335, 1416, 1862
BsaWI	WCCGGW	1 cut(s)	375
BsaXI	ACNNNNNCTCC	2 cut(s)	753, 783
Bsc4I	CCNNNNNNNGG	5 cut(s)	63, 1151, 1190, 1508, 1786
Bse118I	RCCGGY	1 cut(s)	1876
Bse1I	ACTGG	4 cut(s)	784, 1098, 1663, 1968
Bse21I	CCTNAGG	1 cut(s)	909
Bse3DI	GCAATG	2 cut(s)	1833, 2043
BseBI	CCWGG	4 cut(s)	109, 759, 1185, 2250
BseCI	ATCGAT	2 cut(s)	398, 622
BseDI	CCNNGG	6 cut(s)	107, 757, 971, 1335, 1416, 1862
BseGI	GGATG	8 cut(s)	358, 388, 753, 1052, 1795, 1872, 2047, 2130
BseLI	CCNNNNNNNGG	5 cut(s)	63, 1151, 1190, 1508, 1786
BseMI	GCAATG	2 cut(s)	1833, 2043
BseMII	CTCAG	3 cut(s)	188, 900, 1904
BseNI	ACTGG	4 cut(s)	784, 1098, 1663, 1968
BseRI	GAGGAG	4 cut(s)	263, 284, 1167, 1556
BseSI	GKGCMC	3 cut(s)	315, 919, 2164
BseYI	CCCAGC	1 cut(s)	938
BsgI	GTGCAG	2 cut(s)	1578, 1743
Bsh1236I	CGCG	5 cut(s)	11, 71, 456, 541, 1234
BshFI	GGCC	3 cut(s)	80, 487, 1403
BshNI	GGYRCC	3 cut(s)	151, 703, 1331
BshVI	ATCGAT	2 cut(s)	398, 622
BsiHKAI	GWGCWC	7 cut(s)	26, 32, 93, 315, 919, 1641, 1674
BsiHKCI	CYCGRG	2 cut(s)	31, 1992
BsiSI	CCGG	5 cut(s)	155, 376, 1027, 1272, 1877
BslI	CCNNNNNNNGG	5 cut(s)	63, 1151, 1190, 1508, 1786
BsmAI	GTCTC	3 cut(s)	1149, 1911, 1951
BsmI	GAATGC	1 cut(s)	1319
BsnI	GGCC	3 cut(s)	80, 487, 1403
Bso31I	GGTCTC	1 cut(s)	1911
BsoBI	CYCGRG	2 cut(s)	31, 1992
Bsp119I	TTCGAA	1 cut(s)	1323
Bsp1286I	GDGCHC	9 cut(s)	26, 32, 54, 93, 315, 919, 1641, 1674, 2164
Bsp143I	GATC	9 cut(s)	84, 402, 583, 619, 623, 685, 736, 1129, 1180
Bsp19I	CCATGG	2 cut(s)	1335, 1416
BspACI	CCGC	8 cut(s)	11, 14, 456, 1082, 1232, 1825, 1932, 2149
BspANI	GGCC	3 cut(s)	80, 487, 1403
BspCNI	CTCAG	3 cut(s)	187, 901, 1905
BspDI	ATCGAT	2 cut(s)	398, 622
BspFNI	CGCG	5 cut(s)	11, 71, 456, 541, 1234
BspHI	TCATGA	3 cut(s)	688, 1066, 2055
BspLI	GGNNCC	4 cut(s)	153, 705, 1143, 1333
BspMAI	CTGCAG	1 cut(s)	1896
BspMI	ACCTGC	1 cut(s)	1319
BspPI	GGATC	1 cut(s)	1175
BspQI	GCTCTTC	2 cut(s)	14, 171
BspT104I	TTCGAA	1 cut(s)	1323
BspT107I	GGYRCC	3 cut(s)	151, 703, 1331
BspTNI	GGTCTC	1 cut(s)	1911
BsrDI	GCAATG	2 cut(s)	1833, 2043
BsrFI	RCCGGY	1 cut(s)	1876
BsrI	ACTGG	4 cut(s)	784, 1098, 1663, 1968
BssAI	RCCGGY	1 cut(s)	1876
BssECI	CCNNGG	6 cut(s)	107, 757, 971, 1335, 1416, 1862
BssMI	GATC	9 cut(s)	84, 402, 583, 619, 623, 685, 736, 1129, 1180
BssNI	GRCGYC	2 cut(s)	152, 195
BssSI	CACGAG	1 cut(s)	25
BssT1I	CCWWGG	4 cut(s)	971, 1335, 1416, 1862
Bst2BI	CACGAG	1 cut(s)	25
Bst2UI	CCWGG	4 cut(s)	109, 759, 1185, 2250
Bst6I	CTCTTC	2 cut(s)	14, 171
BstACI	GRCGYC	2 cut(s)	152, 195
BstBI	TTCGAA	1 cut(s)	1323
BstC8I	GCNNGC	4 cut(s)	54, 595, 751, 1878
BstDEI	CTNAG	4 cut(s)	174, 891, 909, 1913
BstDSI	CCRYGG	2 cut(s)	1335, 1416
BstENI	CCTNNNNNAGG	1 cut(s)	1149
BstF5I	GGATG	8 cut(s)	358, 388, 753, 1052, 1795, 1872, 2047, 2130
BstFNI	CGCG	5 cut(s)	11, 71, 456, 541, 1234
BstH2I	RGCGCY	1 cut(s)	155
BstHHI	GCGC	3 cut(s)	11, 154, 456
BstKTI	GATC	9 cut(s)	87, 405, 586, 622, 626, 688, 739, 1132, 1183
BstMAI	GTCTC	3 cut(s)	1149, 1911, 1951
BstMBI	GATC	9 cut(s)	84, 402, 583, 619, 623, 685, 736, 1129, 1180
BstMWI	GCNNNNNNNGC	6 cut(s)	77, 676, 1220, 1325, 1931, 2033
BstNI	CCWGG	4 cut(s)	109, 759, 1185, 2250
BstNSI	RCATGY	3 cut(s)	593, 597, 753
BstPAI	GACNNNNGTC	1 cut(s)	1853
BstSCI	CCNGG	5 cut(s)	107, 154, 757, 1183, 2248
BstSFI	CTRYAG	2 cut(s)	1281, 1892
BstSLI	GKGCMC	3 cut(s)	315, 919, 2164
BstUI	CGCG	5 cut(s)	11, 71, 456, 541, 1234
BstV2I	GAAGAC	2 cut(s)	1734, 2247
BstX2I	RGATCY	1 cut(s)	736
BstYI	RGATCY	1 cut(s)	736
Bsu15I	ATCGAT	2 cut(s)	398, 622
Bsu36I	CCTNAGG	1 cut(s)	909
BsuI	GTATCC	1 cut(s)	654
BsuRI	GGCC	3 cut(s)	80, 487, 1403
BsuTUI	ATCGAT	2 cut(s)	398, 622
BtgI	CCRYGG	2 cut(s)	1335, 1416
BtsCI	GGATG	8 cut(s)	358, 388, 753, 1052, 1795, 1872, 2047, 2130
BtsI	GCAGTG	2 cut(s)	315, 2122
BtsIMutI	CAGTG	7 cut(s)	315, 477, 777, 1278, 1357, 1433, 2122
BveI	ACCTGC	1 cut(s)	1319
Cac8I	GCNNGC	4 cut(s)	54, 595, 751, 1878
CaiI	CAGNNNCTG	2 cut(s)	670, 1223
CciI	TCATGA	3 cut(s)	688, 1066, 2055
CfoI	GCGC	3 cut(s)	11, 154, 456
Cfr10I	RCCGGY	1 cut(s)	1876
Cfr13I	GGNCC	3 cut(s)	79, 1013, 1402
ClaI	ATCGAT	2 cut(s)	398, 622
CseI	GACGC	2 cut(s)	184, 295
CsiI	ACCWGGT	1 cut(s)	2248
Csp6I	GTAC	6 cut(s)	4, 73, 1340, 1831, 2080, 2253
CviQI	GTAC	6 cut(s)	4, 73, 1340, 1831, 2080, 2253
DdeI	CTNAG	4 cut(s)	174, 891, 909, 1913
DinI	GGCGCC	1 cut(s)	153
DpnI	GATC	9 cut(s)	86, 404, 585, 621, 625, 687, 738, 1131, 1182
DpnII	GATC	9 cut(s)	84, 402, 583, 619, 623, 685, 736, 1129, 1180
DraIII	CACNNNGTG	1 cut(s)	1721
DrdI	GACNNNNNNGTC	1 cut(s)	1163
DseDI	GACNNNNNNGTC	1 cut(s)	1163
Eam1104I	CTCTTC	2 cut(s)	14, 171
EarI	CTCTTC	2 cut(s)	14, 171
Ecl136II	GAGCTC	2 cut(s)	30, 91
Eco130I	CCWWGG	4 cut(s)	971, 1335, 1416, 1862
Eco147I	AGGCCT	1 cut(s)	487
Eco24I	GRGCYC	3 cut(s)	32, 54, 93
Eco31I	GGTCTC	1 cut(s)	1911
Eco47I	GGWCC	1 cut(s)	1013
Eco53kI	GAGCTC	2 cut(s)	30, 91
Eco57I	CTGAAG	2 cut(s)	702, 1374
Eco81I	CCTNAGG	1 cut(s)	909
Eco88I	CYCGRG	2 cut(s)	31, 1992
EcoICRI	GAGCTC	2 cut(s)	30, 91
EcoNI	CCTNNNNNAGG	1 cut(s)	1149
EcoO109I	RGGNCCY	1 cut(s)	1402
EcoRI	GAATTC	1 cut(s)	983
EcoRII	CCWGG	4 cut(s)	107, 757, 1183, 2248
EcoT14I	CCWWGG	4 cut(s)	971, 1335, 1416, 1862
EcoT22I	ATGCAT	4 cut(s)	595, 751, 755, 1321
EcoT38I	GRGCYC	3 cut(s)	32, 54, 93
EgeI	GGCGCC	1 cut(s)	153
EheI	GGCGCC	1 cut(s)	153
ErhI	CCWWGG	4 cut(s)	971, 1335, 1416, 1862
FalI	AAGNNNNNCTT	2 cut(s)	153, 185
FauI	CCCGC	2 cut(s)	7, 1075
FbaI	TGATCA	1 cut(s)	1129
FokI	GGATG	8 cut(s)	365, 375, 740, 1039, 1802, 1879, 2054, 2137
FriOI	GRGCYC	3 cut(s)	32, 54, 93
FspBI	CTAG	6 cut(s)	866, 927, 972, 1409, 1998, 2272
GlaI	GCGC	3 cut(s)	10, 153, 455
GsaI	CCCAGC	1 cut(s)	942
GsuI	CTGGAG	2 cut(s)	780, 1985
HaeII	RGCGCY	1 cut(s)	155
HaeIII	GGCC	3 cut(s)	80, 487, 1403
HapII	CCGG	5 cut(s)	155, 376, 1027, 1272, 1877
HgaI	GACGC	2 cut(s)	184, 295
HhaI	GCGC	3 cut(s)	11, 154, 456
Hin1I	GRCGYC	2 cut(s)	152, 195
Hin6I	GCGC	3 cut(s)	9, 152, 454
HinP1I	GCGC	3 cut(s)	9, 152, 454
HincII	GTYRAC	3 cut(s)	214, 958, 2132
HindII	GTYRAC	3 cut(s)	214, 958, 2132
HindIII	AAGCTT	2 cut(s)	440, 1772
HinfI	GANTC	9 cut(s)	328, 792, 923, 959, 1275, 1298, 1969, 2063, 2128
HpaII	CCGG	5 cut(s)	155, 376, 1027, 1272, 1877
HphI	GGTGA	6 cut(s)	190, 295, 781, 1190, 1668, 1987
Hpy166II	GTNNAC	6 cut(s)	214, 313, 725, 917, 958, 2132
Hpy188I	TCNGA	9 cut(s)	231, 365, 393, 651, 1003, 1393, 1548, 1636, 2179
Hpy188III	TCNNGA	9 cut(s)	551, 617, 689, 866, 1016, 1067, 1572, 1619, 2056
Hpy8I	GTNNAC	6 cut(s)	214, 313, 725, 917, 958, 2132
Hpy99I	CGWCG	2 cut(s)	101, 200
HpyAV	CCTTC	5 cut(s)	175, 274, 689, 1033, 1780
HpyF10VI	GCNNNNNNNGC	6 cut(s)	77, 676, 1220, 1325, 1931, 2033
HpyF3I	CTNAG	4 cut(s)	174, 891, 909, 1913
Hsp92I	GRCGYC	2 cut(s)	152, 195
HspAI	GCGC	3 cut(s)	9, 152, 454
KasI	GGCGCC	1 cut(s)	151
KroI	GCCGGC	1 cut(s)	1876
KroNI	GCCGGC	1 cut(s)	1878
Ksp22I	TGATCA	1 cut(s)	1129
Kzo9I	GATC	9 cut(s)	84, 402, 583, 619, 623, 685, 736, 1129, 1180
LguI	GCTCTTC	2 cut(s)	14, 171
LmnI	GCTCC	1 cut(s)	667
LweI	GCATC	7 cut(s)	736, 762, 1405, 1721, 1780, 1803, 2023
MabI	ACCWGGT	1 cut(s)	2248
MaeI	CTAG	6 cut(s)	866, 927, 972, 1409, 1998, 2272
MaeIII	GTNAC	6 cut(s)	283, 341, 769, 775, 894, 1647
MalI	GATC	9 cut(s)	86, 404, 585, 621, 625, 687, 738, 1131, 1182
MboI	GATC	9 cut(s)	84, 402, 583, 619, 623, 685, 736, 1129, 1180
MflI	RGATCY	1 cut(s)	736
MhlI	GDGCHC	9 cut(s)	26, 32, 54, 93, 315, 919, 1641, 1674, 2164
MluCI	AATT	6 cut(s)	802, 879, 983, 1373, 1898, 2019
MluI	ACGCGT	1 cut(s)	539
Mly113I	GGCGCC	1 cut(s)	152
MlyI	GAGTC	6 cut(s)	337, 801, 953, 1269, 1978, 2137
MmeI	TCCRAC	2 cut(s)	246, 1983
Mph1103I	ATGCAT	4 cut(s)	595, 751, 755, 1321
MroNI	GCCGGC	1 cut(s)	1876
MroXI	GAANNNNTTC	1 cut(s)	165
MseI	TTAA	8 cut(s)	369, 419, 798, 882, 1077, 2009, 2156, 2166
MslI	CAYNNNNRTG	2 cut(s)	1125, 1736
MspA1I	CMGCKG	3 cut(s)	1223, 1682, 1934
MspI	CCGG	5 cut(s)	155, 376, 1027, 1272, 1877
MspR9I	CCNGG	5 cut(s)	109, 156, 759, 1185, 2250
Mva1269I	GAATGC	1 cut(s)	1319
MvaI	CCWGG	4 cut(s)	109, 759, 1185, 2250
MvnI	CGCG	5 cut(s)	11, 71, 456, 541, 1234
MwoI	GCNNNNNNNGC	6 cut(s)	77, 676, 1220, 1325, 1931, 2033
NaeI	GCCGGC	1 cut(s)	1878
NarI	GGCGCC	1 cut(s)	152
NciI	CCSGG	1 cut(s)	156
NcoI	CCATGG	2 cut(s)	1335, 1416
NdeII	GATC	9 cut(s)	84, 402, 583, 619, 623, 685, 736, 1129, 1180
NgoMIV	GCCGGC	1 cut(s)	1876
NlaIV	GGNNCC	4 cut(s)	153, 705, 1143, 1333
NmeAIII	GCCGAG	2 cut(s)	72, 82
NmuCI	GTSAC	5 cut(s)	283, 769, 775, 894, 1647
NsiI	ATGCAT	4 cut(s)	595, 751, 755, 1321
NspI	RCATGY	3 cut(s)	593, 597, 753
NspV	TTCGAA	1 cut(s)	1323
PaeI	GCATGC	2 cut(s)	597, 753
PaeR7I	CTCGAG	2 cut(s)	31, 1992
PagI	TCATGA	3 cut(s)	688, 1066, 2055
PaqCI	CACCTGC	1 cut(s)	1319
PceI	AGGCCT	1 cut(s)	487
PciSI	GCTCTTC	2 cut(s)	14, 171
PcsI	WCGNNNNNNNCGW	2 cut(s)	93, 192
PctI	GAATGC	1 cut(s)	1319
PdiI	GCCGGC	1 cut(s)	1878
PdmI	GAANNNNTTC	1 cut(s)	165
PfeI	GAWTC	3 cut(s)	923, 1298, 2063
PflFI	GACNNNGTC	1 cut(s)	194
PfoI	TCCNGGA	1 cut(s)	1183
PleI	GAGTC	6 cut(s)	336, 800, 953, 1269, 1977, 2136
PluTI	GGCGCC	1 cut(s)	155
PpsI	GAGTC	6 cut(s)	336, 800, 953, 1269, 1977, 2136
PshAI	GACNNNNGTC	1 cut(s)	1853
PsiI	TTATAA	1 cut(s)	1518
Psp124BI	GAGCTC	2 cut(s)	32, 93
Psp6I	CCWGG	4 cut(s)	107, 757, 1183, 2248
PspFI	CCCAGC	1 cut(s)	938
PspGI	CCWGG	4 cut(s)	107, 757, 1183, 2248
PspN4I	GGNNCC	4 cut(s)	153, 705, 1143, 1333
PspPI	GGNCC	3 cut(s)	79, 1013, 1402
PspXI	VCTCGAGB	2 cut(s)	31, 1992
PstI	CTGCAG	1 cut(s)	1896
PstNI	CAGNNNCTG	2 cut(s)	670, 1223
PsuI	RGATCY	1 cut(s)	736
PsyI	GACNNNGTC	1 cut(s)	194
PvuII	CAGCTG	2 cut(s)	1223, 1682
RsaI	GTAC	6 cut(s)	5, 74, 1341, 1832, 2081, 2254
RsaNI	GTAC	6 cut(s)	4, 73, 1340, 1831, 2080, 2253
RseI	CAYNNNNRTG	2 cut(s)	1125, 1736
SacI	GAGCTC	2 cut(s)	32, 93
SapI	GCTCTTC	2 cut(s)	14, 171
SaqAI	TTAA	8 cut(s)	369, 419, 798, 882, 1077, 2009, 2156, 2166
Sau3AI	GATC	9 cut(s)	84, 402, 583, 619, 623, 685, 736, 1129, 1180
Sau96I	GGNCC	3 cut(s)	79, 1013, 1402
SchI	GAGTC	6 cut(s)	337, 801, 953, 1269, 1978, 2137
ScrFI	CCNGG	5 cut(s)	109, 156, 759, 1185, 2250
SduI	GDGCHC	9 cut(s)	26, 32, 54, 93, 315, 919, 1641, 1674, 2164
SexAI	ACCWGGT	1 cut(s)	2248
SfaNI	GCATC	7 cut(s)	736, 762, 1405, 1721, 1780, 1803, 2023
SfcI	CTRYAG	2 cut(s)	1281, 1892
SfoI	GGCGCC	1 cut(s)	153
Sfr274I	CTCGAG	2 cut(s)	31, 1992
SfuI	TTCGAA	1 cut(s)	1323
SinI	GGWCC	1 cut(s)	1013
SlaI	CTCGAG	2 cut(s)	31, 1992
SmiMI	CAYNNNNRTG	2 cut(s)	1125, 1736
SmlI	CTYRAG	2 cut(s)	31, 1992
SmoI	CTYRAG	2 cut(s)	31, 1992
SphI	GCATGC	2 cut(s)	597, 753
Sse9I	AATT	6 cut(s)	802, 879, 983, 1373, 1898, 2019
SseBI	AGGCCT	1 cut(s)	487
SsiI	CCGC	8 cut(s)	11, 14, 456, 1082, 1232, 1825, 1932, 2149
SspDI	GGCGCC	1 cut(s)	151
SspMI	CTAG	6 cut(s)	866, 927, 972, 1409, 1998, 2272
SstI	GAGCTC	2 cut(s)	32, 93
StuI	AGGCCT	1 cut(s)	487
StyD4I	CCNGG	5 cut(s)	107, 154, 757, 1183, 2248
StyI	CCWWGG	4 cut(s)	971, 1335, 1416, 1862
TaqI	TCGA	7 cut(s)	32, 87, 398, 622, 709, 1323, 1993
TasI	AATT	6 cut(s)	802, 879, 983, 1373, 1898, 2019
TatI	WGTACW	2 cut(s)	1830, 2079
TauI	GCSGC	1 cut(s)	14
TfiI	GAWTC	3 cut(s)	923, 1298, 2063
Tru1I	TTAA	8 cut(s)	369, 419, 798, 882, 1077, 2009, 2156, 2166
Tru9I	TTAA	8 cut(s)	369, 419, 798, 882, 1077, 2009, 2156, 2166
TscAI	CASTG	7 cut(s)	315, 484, 784, 1285, 1357, 1433, 2122
TseFI	GTSAC	5 cut(s)	283, 769, 775, 894, 1647
Tsp45I	GTSAC	5 cut(s)	283, 769, 775, 894, 1647
TspDTI	ATGAA	7 cut(s)	705, 976, 1151, 1554, 1579, 1626, 1643
TspRI	CASTG	7 cut(s)	315, 484, 784, 1285, 1357, 1433, 2122
Tth111I	GACNNNGTC	1 cut(s)	194
VneI	GTGCAC	2 cut(s)	311, 915
VpaK11BI	GGWCC	1 cut(s)	1013
XagI	CCTNNNNNAGG	1 cut(s)	1149
XapI	RAATTY	3 cut(s)	983, 1373, 1898
XbaI	TCTAGA	1 cut(s)	865
XceI	RCATGY	3 cut(s)	593, 597, 753
XhoI	CTCGAG	2 cut(s)	31, 1992
XmaJI	CCTAGG	1 cut(s)	971
XmnI	GAANNNNTTC	1 cut(s)	165
XspI	CTAG	6 cut(s)	866, 927, 972, 1409, 1998, 2272
Zsp2I	ATGCAT	4 cut(s)	595, 751, 755, 1321

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.