Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0013292

Physical Location & Seq

Genomic Coordinates Forward (+)

19194588 .. 19202722

Gene Structure 8135 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1104500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2388 bp

ATGTACGGAGCACAACGGAGAAGCTTAAGCTCGAACTACGGCTCCTCCTACGCACCTGATCTCCACACCATCTGGCCTCTCTACTTTGCTCAAATCGAGCTCGGCGACGACAAGCCATGGCTCAGTCTCACACACTCCCTCATCGGCTTCTTCTCCACGACGCCGGGGCAAGCTCTCCTTTCCCAGGTGAAGGATGATGGTGGTGTGGCGGTGTTGCCGATTGATTTCGAGCAATTTCGAAAAATTTGTGATGTTGAGGAGTTTTATGAAGCTTTAGAGGAGAAACCCAAGATAACCCTCTTATGCATGAGTGCTGCAGTTCACAAAGTCCTGCTGACTCAATGGGACAATTGCAGGGTAGAGGACGGTACCAAAATTAATATTCGATTGCATAACTACCCCGAATCCATGATTGCCTTGAAGAACTTAAAAGCTGCATGTATTGACAAGATTGTATCTGTGCGTGGTACAGTAGTAAAAGTTAGCAATGTCAAACCTTTTGTGGTGCAAATGGATTTTGACTGTGTGAAGTGTAAAACAAGTATTACACGCATGTGTCCTGATGGGAAATTTTCACCGCCAACAACATGCGATCTAAATGGGTGCAAGAGCAGAAATTTTGACCCCATTCGATCTACTGCTGTGACGATAGATTTTCAGAAGATAAGGATACAGGAGCTACTAAAGCCTGAAGATCATGAAGAAGGCAGGATGCCTCGAACAGTAGAATGTGAACTTTTTGAAGATCTTGTTGATGCATGCATCCCAGGAGATGTGGTGACTCTCACTGGGATTATAAGAGTCATTAACAATTACATGGACATTGGAGGAGGCAAATCGAAGGGAAAAACTCAAGGAATTTACTATTTGTATCTAGAAGCTATTTCCATTAAAAACACAAAGTCACAATCTATGCCCGAGGATTTACAAGATTCTAGTTCTAATGACAGACCTATGGCACTTGTTGATCTTTCCTCATTTTCCCCAAGGGATTTGGAATTCATTGTGAAGTTCACAGAGGAACATGGTCCAGATACCTTCAGGCAACTACTTCAATCTATTTGTCCATCAATCTTTGGTCACGAGCTTGTTAAAGCGGGAATATTGCTGGCACTCTTTGGAGGTGTGCGGAAGAATTCAAATGATCAGAACAAGGTTCCTGTTAGAGGAGATATCCATGTCATAATTGTTGGTGATCCCGGACTAGGCAAGAGCCAGCTTCTCCAAGCAGCGGCAGCCATTTCTCCACGAGGCATTTATGTGTGTGGTAATGCCACAACCAAAGCTGGCCTCACTGTTGCTGTTGTAAAGGATCCTATGACAAGTGATTATGGTTTTGAAGCTGGTGCAATGGTAATTGCGGACAGTGGGTTGTGCTGTATTGATGAGTTTGACAAAATGTCTTCAGAGCATCAGGCTCTACTGGAAGCCATGGAACAACAGTGTGTCTCAGTCGCAAAGGCTGGACTGGTGGCAAGTTTGTCGGCACGAACTTCTGTCTTAGCAGCTGCAAATCCTGTTGGTGGTCATTATAACCGTGCAAAAACAGTGAATGAAAATTTGAAAATAAGTGCTGCTCTCTTATCGCGATTTGATTTGGTTTTCATATTACTTGACAAACCTAATGAACTTTTGGATAAGCGAGTCTCAGAACACATCATGTCACTTCATGCTGGATCTAGAGAAAATTCGCCAGCTGCAAAAAAGATTCGTAGAGCATCACAGAGTGCGGAAGGCATAGATATTAGTGGAGAAGGTGGTTCTTTGGTTTCAAGATTGAGGCTTGACCCGAAGAAGGATGAGGATTTTGTTCCATTACCAGTACAACTCCTCCGCAAGTATATTGCTTATGCAAGAACTTTTGTGTTTCCTAGGATGTCAAAGCCTGCAGCAGAAATCCTGCAAAAGTTTTACTTAAAACTGAGAGACCATGCGACATCTGCTGATAGCACACCAATAACAGCAAGGCAATTAGAGAGTCTGGTGAGGCTGACCGAAGCTCGAGCGAGGCTAGATTTGAGAGAAGAAATAACAGCCCAAGATGCAATGGATGTGGTTGAAATCATGAGAGAATCCTTGTATGACAAGTACATTGATGAGCATGGTGTTGTGGATTTTGGTCGGAGTGGAGGGATGAGTCAACAAAAAGAAGCAAAACGGTTCTTAAGTGCCCTCAATAGGCAATCAGAGTTGCAGCAGAAAGACTGTTTTACAATTTCTGAAATATACAGTTTGGCAGATAGGATTGGTCTAAGAGTTCCAGACATCGACACATTTGTTGACAACTTAAACAGTGTTGGTTATCTACTGAAGAAAGGAGCAAAGACATACCAGGTACTATCATCGTCTTATTCTCGGAGTCAATCGTCCTCAAGATCAAGAGGCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000280 GO:0000724 GO:0000725 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006259 GO:0006281 GO:0006302 GO:0006310 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007049 GO:0007140 GO:0007143 GO:0007275 GO:0007276 GO:0007292 GO:0008150 GO:0008152 GO:0009555 GO:0009987 GO:0016043 GO:0019953 GO:0022402 GO:0022412 GO:0022414 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032504 GO:0032991 GO:0033554 GO:0034641 GO:0042555 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0044703 GO:0046483 GO:0048229 GO:0048232 GO:0048285 GO:0048609 GO:0048856 GO:0050896 GO:0051321 GO:0051704 GO:0051716 GO:0070013 GO:0071704 GO:0071840 GO:0090304 GO:0097362 GO:0140013 GO:1901360 GO:1903046

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MCM MCM_OB

Protein Analysis

796

Amino Acids

87.95

Weight (kDa)

6.57

Isoelectric Point (pI)

47.44

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MCM_OB	PF17207	140 - 269	9.1e-28	MCM OB domain
MCM	PF00493	343 - 557	4.4e-89	MCM P-loop domain
AAA_5	PF07728	393 - 531	1.9e-07	AAA domain (dynein-related subfamily)
MCM_lid	PF17855	610 - 693	6e-26	MCM AAA-lid domain
WHD_MCM8	PF25051	705 - 780	5.3e-31	MCM8 winged helix domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0013292)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G09660 AT3G09660 AT3G09660
fragaria_vesca	FvH4_6g15280 FvH4_6g15280
malus_domestica	MD04G1104500.v1.1
prunus_persica	Prupe.6G241500_v2.0.a1 Prupe.6G241500_v2.0.a1
pyrus_communis	pycom04g09950
rosa_chinensis	RchiOBHm_Chr3g0468711
rosa_laevigata	RLG00000024414
rosa_multiflora	Rmu_sc0011538.1_g000001
rosa_roxburghii	Rroxscaffold_6G00412640
rosa_rugosa	Rorug03G0096200
rosa_samantha	Rh3AG145200 Rh3BG167800 Rh3DG166500
rosa_wichuraiana	Rw3G013700

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	797, 1533
Acc65I	GGTACC	1 cut(s)	368
AccB1I	GGYRCC	1 cut(s)	368
AccII	CGCG	1 cut(s)	1588
AciI	CCGC	8 cut(s)	209, 578, 1097, 1129, 1232, 1361, 1730, 1834
AclWI	GGATC	4 cut(s)	1190, 1307, 1320, 1684
AcsI	RAATTY	8 cut(s)	243, 569, 616, 858, 998, 1135, 1558, 1687
AcuI	CTGAAG	4 cut(s)	711, 1024, 1389, 2330
AcyI	GRCGYC	1 cut(s)	161
AdeI	CACNNNGTG	1 cut(s)	1727
AfaI	GTAC	6 cut(s)	5, 370, 469, 1824, 2090, 2337
AfiI	CCNNNNNNNGG	8 cut(s)	184, 190, 1166, 1205, 1231, 1523, 1795, 2178
AflII	CTTAAG	2 cut(s)	25, 2164
AgsI	TTSAA	8 cut(s)	421, 743, 1055, 1140, 1340, 1564, 1773, 2060
AhdI	GACNNNNNGTC	1 cut(s)	1399
AjnI	CCWGG	3 cut(s)	183, 766, 2331
AleI	CACNNNNGTG	1 cut(s)	553
AloI	GAACNNNNNNTCC	2 cut(s)	26, 58
Alw21I	GWGCWC	2 cut(s)	13, 102
Alw26I	GTCTC	4 cut(s)	131, 1453, 1651, 1920
AlwI	GGATC	4 cut(s)	1190, 1307, 1320, 1684
Ama87I	CYCGRG	2 cut(s)	917, 2001
AoxI	GGCC	2 cut(s)	74, 1288
ApoI	RAATTY	8 cut(s)	243, 569, 616, 858, 998, 1135, 1558, 1687
AseI	ATTAAT	1 cut(s)	378
Asp718I	GGTACC	1 cut(s)	368
AspA2I	CCTAGG	1 cut(s)	1871
AspS9I	GGNCC	1 cut(s)	1028
AsuC2I	CCSGG	2 cut(s)	165, 1200
AsuHPI	GGTGA	5 cut(s)	199, 567, 790, 1205, 1996
AsuII	TTCGAA	1 cut(s)	238
AvaI	CYCGRG	2 cut(s)	917, 2001
AvaII	GGWCC	1 cut(s)	1028
AvrII	CCTAGG	1 cut(s)	1871
BaeGI	GKGCMC	1 cut(s)	2173
BaeI	ACNNNNGTAYC	2 cut(s)	459, 492
BamHI	GGATCC	1 cut(s)	1312
BanI	GGYRCC	1 cut(s)	368
BanII	GRGCYC	1 cut(s)	102
BauI	CACGAG	2 cut(s)	1082, 1248
BbsI	GAAGAC	1 cut(s)	1395
Bbv12I	GWGCWC	2 cut(s)	13, 102
BccI	CCATC	4 cut(s)	77, 191, 557, 1075
BceAI	ACGGC	1 cut(s)	55
BcgI	CGANNNNNNTGC	2 cut(s)	1464, 1498
BciT130I	CCWGG	3 cut(s)	185, 768, 2333
BciVI	GTATCC	1 cut(s)	663
BclI	TGATCA	1 cut(s)	1144
BcnI	CCSGG	2 cut(s)	165, 1200
BcoDI	GTCTC	4 cut(s)	131, 1453, 1651, 1920
BfaI	CTAG	6 cut(s)	875, 936, 1205, 1680, 1872, 2012
BfmI	CTRYAG	2 cut(s)	315, 1887
BfrI	CTTAAG	2 cut(s)	25, 2164
BfuI	GTATCC	1 cut(s)	663
BglII	AGATCT	1 cut(s)	745
BlnI	CCTAGG	1 cut(s)	1871
Bme1390I	CCNGG	5 cut(s)	165, 185, 768, 1200, 2333
Bme18I	GGWCC	1 cut(s)	1028
BmeRI	GACNNNNNGTC	1 cut(s)	1399
BmeT110I	CYCGRG	2 cut(s)	917, 2001
BmgT120I	GGNCC	1 cut(s)	1028
BmiI	GGNNCC	4 cut(s)	43, 370, 1158, 1314
BmrFI	CCNGG	5 cut(s)	165, 185, 768, 1200, 2333
BmrI	ACTGGG	1 cut(s)	798
BmsI	GCATC	6 cut(s)	702, 745, 771, 1420, 1727, 2032
BmuI	ACTGGG	1 cut(s)	798
BpiI	GAAGAC	1 cut(s)	1395
Bpu14I	TTCGAA	1 cut(s)	238
BpuEI	CTTGAG	2 cut(s)	837, 2356
BpuMI	CCSGG	2 cut(s)	165, 1200
BsaHI	GRCGYC	1 cut(s)	161
BsaI	GGTCTC	1 cut(s)	1920
BsaJI	CCNNGG	8 cut(s)	116, 164, 183, 766, 918, 986, 1431, 1871
Bsc4I	CCNNNNNNNGG	8 cut(s)	184, 190, 1166, 1205, 1231, 1523, 1795, 2178
Bse1I	ACTGG	4 cut(s)	793, 1428, 1473, 1820
Bse3DI	GCAATG	3 cut(s)	493, 1356, 2052
BseBI	CCWGG	3 cut(s)	185, 768, 2333
BseDI	CCNNGG	8 cut(s)	116, 164, 183, 766, 918, 986, 1431, 1871
BseGI	GGATG	7 cut(s)	199, 717, 762, 1804, 1881, 2056, 2139
BseLI	CCNNNNNNNGG	8 cut(s)	184, 190, 1166, 1205, 1231, 1523, 1795, 2178
BseMI	GCAATG	3 cut(s)	493, 1356, 2052
BseMII	CTCAG	4 cut(s)	136, 1464, 1662, 1913
BseNI	ACTGG	4 cut(s)	793, 1428, 1473, 1820
BseRI	GAGGAG	6 cut(s)	34, 272, 293, 843, 1182, 1820
BseSI	GKGCMC	1 cut(s)	2173
Bsh1236I	CGCG	1 cut(s)	1588
BshFI	GGCC	2 cut(s)	76, 1290
BshNI	GGYRCC	1 cut(s)	368
BsiHKAI	GWGCWC	2 cut(s)	13, 102
BsiHKCI	CYCGRG	2 cut(s)	917, 2001
BsiSI	CCGG	2 cut(s)	164, 1200
BslFI	GGGAC	1 cut(s)	359
BslI	CCNNNNNNNGG	8 cut(s)	184, 190, 1166, 1205, 1231, 1523, 1795, 2178
BsmAI	GTCTC	4 cut(s)	131, 1453, 1651, 1920
BsmFI	GGGAC	1 cut(s)	359
BsnI	GGCC	2 cut(s)	76, 1290
Bso31I	GGTCTC	1 cut(s)	1920
BsoBI	CYCGRG	2 cut(s)	917, 2001
Bsp119I	TTCGAA	1 cut(s)	238
Bsp1286I	GDGCHC	3 cut(s)	13, 102, 2173
Bsp19I	CCATGG	2 cut(s)	116, 1431
Bsp68I	TCGCGA	1 cut(s)	1588
BspACI	CCGC	8 cut(s)	209, 578, 1097, 1129, 1232, 1361, 1730, 1834
BspANI	GGCC	2 cut(s)	76, 1290
BspCNI	CTCAG	4 cut(s)	135, 1463, 1661, 1914
BspFNI	CGCG	1 cut(s)	1588
BspHI	TCATGA	2 cut(s)	697, 2064
BspLI	GGNNCC	4 cut(s)	43, 370, 1158, 1314
BspMAI	CTGCAG	2 cut(s)	319, 1891
BspPI	GGATC	4 cut(s)	1190, 1307, 1320, 1684
BspT104I	TTCGAA	1 cut(s)	238
BspT107I	GGYRCC	1 cut(s)	368
BspTI	CTTAAG	2 cut(s)	25, 2164
BspTNI	GGTCTC	1 cut(s)	1920
BsrDI	GCAATG	3 cut(s)	493, 1356, 2052
BsrI	ACTGG	4 cut(s)	793, 1428, 1473, 1820
BssECI	CCNNGG	8 cut(s)	116, 164, 183, 766, 918, 986, 1431, 1871
BssNI	GRCGYC	1 cut(s)	161
BssSI	CACGAG	2 cut(s)	1082, 1248
BssT1I	CCWWGG	4 cut(s)	116, 986, 1431, 1871
Bst2BI	CACGAG	2 cut(s)	1082, 1248
Bst2UI	CCWGG	3 cut(s)	185, 768, 2333
BstACI	GRCGYC	1 cut(s)	161
BstAFI	CTTAAG	2 cut(s)	25, 2164
BstBI	TTCGAA	1 cut(s)	238
BstC8I	GCNNGC	7 cut(s)	171, 760, 1110, 1217, 1288, 1695, 1887
BstDEI	CTNAG	6 cut(s)	122, 1450, 1501, 1648, 1922, 2252
BstDSI	CCRYGG	2 cut(s)	116, 1431
BstENI	CCTNNNNNAGG	2 cut(s)	182, 1164
BstF5I	GGATG	7 cut(s)	199, 717, 762, 1804, 1881, 2056, 2139
BstFNI	CGCG	1 cut(s)	1588
BstMAI	GTCTC	4 cut(s)	131, 1453, 1651, 1920
BstMWI	GCNNNNNNNGC	4 cut(s)	685, 1235, 1940, 2042
BstNI	CCWGG	3 cut(s)	185, 768, 2333
BstNSI	RCATGY	4 cut(s)	441, 556, 591, 762
BstSCI	CCNGG	5 cut(s)	163, 183, 766, 1198, 2331
BstSFI	CTRYAG	2 cut(s)	315, 1887
BstSLI	GKGCMC	1 cut(s)	2173
BstUI	CGCG	1 cut(s)	1588
BstV2I	GAAGAC	1 cut(s)	1395
BstX2I	RGATCY	3 cut(s)	745, 1312, 1676
BstYI	RGATCY	3 cut(s)	745, 1312, 1676
BsuI	GTATCC	1 cut(s)	663
BsuRI	GGCC	2 cut(s)	76, 1290
BtgI	CCRYGG	2 cut(s)	116, 1431
BtsCI	GGATG	7 cut(s)	199, 717, 762, 1804, 1881, 2056, 2139
BtsIMutI	CAGTG	6 cut(s)	786, 1293, 1372, 1448, 1554, 2299
BtuMI	TCGCGA	1 cut(s)	1588
Cac8I	GCNNGC	7 cut(s)	171, 760, 1110, 1217, 1288, 1695, 1887
CciI	TCATGA	2 cut(s)	697, 2064
Cfr13I	GGNCC	1 cut(s)	1028
CseI	GACGC	1 cut(s)	169
CsiI	ACCWGGT	1 cut(s)	2331
Csp6I	GTAC	6 cut(s)	4, 369, 468, 1823, 2089, 2336
CspCI	CAANNNNNGTGG	2 cut(s)	2034, 2069
CviQI	GTAC	6 cut(s)	4, 369, 468, 1823, 2089, 2336
DdeI	CTNAG	6 cut(s)	122, 1450, 1501, 1648, 1922, 2252
DraIII	CACNNNGTG	1 cut(s)	1727
DriI	GACNNNNNGTC	1 cut(s)	1399
Eam1105I	GACNNNNNGTC	1 cut(s)	1399
Ecl136II	GAGCTC	1 cut(s)	100
Eco130I	CCWWGG	4 cut(s)	116, 986, 1431, 1871
Eco24I	GRGCYC	1 cut(s)	102
Eco31I	GGTCTC	1 cut(s)	1920
Eco32I	GATATC	1 cut(s)	1174
Eco47I	GGWCC	1 cut(s)	1028
Eco53kI	GAGCTC	1 cut(s)	100
Eco57I	CTGAAG	4 cut(s)	711, 1024, 1389, 2330
Eco88I	CYCGRG	2 cut(s)	917, 2001
EcoICRI	GAGCTC	1 cut(s)	100
EcoNI	CCTNNNNNAGG	2 cut(s)	182, 1164
EcoRI	GAATTC	2 cut(s)	998, 1135
EcoRII	CCWGG	3 cut(s)	183, 766, 2331
EcoRV	GATATC	1 cut(s)	1174
EcoT14I	CCWWGG	4 cut(s)	116, 986, 1431, 1871
EcoT22I	ATGCAT	3 cut(s)	308, 760, 764
EcoT38I	GRGCYC	1 cut(s)	102
ErhI	CCWWGG	4 cut(s)	116, 986, 1431, 1871
FalI	AAGNNNNNCTT	4 cut(s)	162, 194, 1898, 1930
FaqI	GGGAC	1 cut(s)	359
FauI	CCCGC	1 cut(s)	1090
FbaI	TGATCA	1 cut(s)	1144
FokI	GGATG	7 cut(s)	206, 724, 749, 1811, 1888, 2063, 2146
FriOI	GRGCYC	1 cut(s)	102
FspBI	CTAG	6 cut(s)	875, 936, 1205, 1680, 1872, 2012
HaeIII	GGCC	2 cut(s)	76, 1290
HapII	CCGG	2 cut(s)	164, 1200
HgaI	GACGC	1 cut(s)	169
Hin1I	GRCGYC	1 cut(s)	161
HincII	GTYRAC	2 cut(s)	2141, 2281
HindII	GTYRAC	2 cut(s)	2141, 2281
HindIII	AAGCTT	2 cut(s)	22, 270
HpaII	CCGG	2 cut(s)	164, 1200
HphI	GGTGA	5 cut(s)	199, 567, 790, 1205, 1996
Hpy166II	GTNNAC	5 cut(s)	322, 734, 1014, 2141, 2281
Hpy188I	TCNGA	8 cut(s)	660, 1149, 1408, 1651, 2124, 2188, 2221, 2358
Hpy8I	GTNNAC	5 cut(s)	322, 734, 1014, 2141, 2281
Hpy99I	CGWCG	2 cut(s)	110, 163
HpyAV	CCTTC	7 cut(s)	184, 698, 835, 1048, 1727, 1748, 1789
HpyF10VI	GCNNNNNNNGC	4 cut(s)	685, 1235, 1940, 2042
HpyF3I	CTNAG	6 cut(s)	122, 1450, 1501, 1648, 1922, 2252
Hsp92I	GRCGYC	1 cut(s)	161
KpnI	GGTACC	1 cut(s)	372
Ksp22I	TGATCA	1 cut(s)	1144
LmnI	GCTCC	4 cut(s)	8, 47, 676, 2318
LweI	GCATC	6 cut(s)	702, 745, 771, 1420, 1727, 2032
MabI	ACCWGGT	1 cut(s)	2331
MaeI	CTAG	6 cut(s)	875, 936, 1205, 1680, 1872, 2012
MaeIII	GTNAC	5 cut(s)	643, 778, 903, 1079, 1662
MfeI	CAATTG	1 cut(s)	349
MflI	RGATCY	3 cut(s)	745, 1312, 1676
MhlI	GDGCHC	3 cut(s)	13, 102, 2173
MlyI	GAGTC	7 cut(s)	331, 775, 810, 1653, 1987, 2146, 2368
MmeI	TCCRAC	1 cut(s)	2102
Mph1103I	ATGCAT	3 cut(s)	308, 760, 764
MseI	TTAA	9 cut(s)	26, 378, 428, 807, 891, 1092, 1916, 2165, 2288
MslI	CAYNNNNRTG	2 cut(s)	553, 1259
MspA1I	CMGCKG	3 cut(s)	1232, 1508, 1697
MspCI	CTTAAG	2 cut(s)	25, 2164
MspI	CCGG	2 cut(s)	164, 1200
MspR9I	CCNGG	5 cut(s)	165, 185, 768, 1200, 2333
MunI	CAATTG	1 cut(s)	349
MvaI	CCWGG	3 cut(s)	185, 768, 2333
MvnI	CGCG	1 cut(s)	1588
MwoI	GCNNNNNNNGC	4 cut(s)	685, 1235, 1940, 2042
NciI	CCSGG	2 cut(s)	165, 1200
NcoI	CCATGG	2 cut(s)	116, 1431
NlaIV	GGNNCC	4 cut(s)	43, 370, 1158, 1314
NmeAIII	GCCGAG	1 cut(s)	81
NmuCI	GTSAC	5 cut(s)	643, 778, 903, 1079, 1662
NruI	TCGCGA	1 cut(s)	1588
NsiI	ATGCAT	3 cut(s)	308, 760, 764
NspI	RCATGY	4 cut(s)	441, 556, 591, 762
NspV	TTCGAA	1 cut(s)	238
OliI	CACNNNNGTG	1 cut(s)	553
PaeI	GCATGC	1 cut(s)	762
PaeR7I	CTCGAG	1 cut(s)	2001
PagI	TCATGA	2 cut(s)	697, 2064
PcsI	WCGNNNNNNNCGW	1 cut(s)	102
PfeI	GAWTC	4 cut(s)	404, 932, 1708, 2072
PfoI	TCCNGGA	1 cut(s)	1198
PleI	GAGTC	7 cut(s)	331, 775, 809, 1652, 1986, 2145, 2367
PpsI	GAGTC	7 cut(s)	331, 775, 809, 1652, 1986, 2145, 2367
PshBI	ATTAAT	1 cut(s)	378
PsiI	TTATAA	2 cut(s)	797, 1533
Psp124BI	GAGCTC	1 cut(s)	102
Psp6I	CCWGG	3 cut(s)	183, 766, 2331
PspGI	CCWGG	3 cut(s)	183, 766, 2331
PspN4I	GGNNCC	4 cut(s)	43, 370, 1158, 1314
PspPI	GGNCC	1 cut(s)	1028
PspXI	VCTCGAGB	1 cut(s)	2001
PstI	CTGCAG	2 cut(s)	319, 1891
PsuI	RGATCY	3 cut(s)	745, 1312, 1676
PvuII	CAGCTG	2 cut(s)	1508, 1697
RruI	TCGCGA	1 cut(s)	1588
RsaI	GTAC	6 cut(s)	5, 370, 469, 1824, 2090, 2337
RsaNI	GTAC	6 cut(s)	4, 369, 468, 1823, 2089, 2336
RseI	CAYNNNNRTG	2 cut(s)	553, 1259
SacI	GAGCTC	1 cut(s)	102
SaqAI	TTAA	9 cut(s)	26, 378, 428, 807, 891, 1092, 1916, 2165, 2288
Sau96I	GGNCC	1 cut(s)	1028
SchI	GAGTC	7 cut(s)	331, 775, 810, 1653, 1987, 2146, 2368
ScrFI	CCNGG	5 cut(s)	165, 185, 768, 1200, 2333
SduI	GDGCHC	3 cut(s)	13, 102, 2173
SexAI	ACCWGGT	1 cut(s)	2331
SfaNI	GCATC	6 cut(s)	702, 745, 771, 1420, 1727, 2032
SfcI	CTRYAG	2 cut(s)	315, 1887
Sfr274I	CTCGAG	1 cut(s)	2001
SfuI	TTCGAA	1 cut(s)	238
SinI	GGWCC	1 cut(s)	1028
SlaI	CTCGAG	1 cut(s)	2001
SmiMI	CAYNNNNRTG	2 cut(s)	553, 1259
SmlI	CTYRAG	5 cut(s)	25, 852, 2001, 2164, 2371
SmoI	CTYRAG	5 cut(s)	25, 852, 2001, 2164, 2371
SphI	GCATGC	1 cut(s)	762
SsiI	CCGC	8 cut(s)	209, 578, 1097, 1129, 1232, 1361, 1730, 1834
SspI	AATATT	2 cut(s)	382, 1104
SspMI	CTAG	6 cut(s)	875, 936, 1205, 1680, 1872, 2012
SstI	GAGCTC	1 cut(s)	102
StyD4I	CCNGG	5 cut(s)	163, 183, 766, 1198, 2331
StyI	CCWWGG	4 cut(s)	116, 986, 1431, 1871
TaqII	GACCGA	1 cut(s)	2009
TatI	WGTACW	2 cut(s)	1822, 2088
TauI	GCSGC	1 cut(s)	1235
TfiI	GAWTC	4 cut(s)	404, 932, 1708, 2072
Tru1I	TTAA	9 cut(s)	26, 378, 428, 807, 891, 1092, 1916, 2165, 2288
Tru9I	TTAA	9 cut(s)	26, 378, 428, 807, 891, 1092, 1916, 2165, 2288
TscAI	CASTG	6 cut(s)	793, 1300, 1372, 1448, 1554, 2299
TseFI	GTSAC	5 cut(s)	643, 778, 903, 1079, 1662
Tsp45I	GTSAC	5 cut(s)	643, 778, 903, 1079, 1662
TspDTI	ATGAA	7 cut(s)	282, 714, 991, 1569, 1594, 1641, 1658
TspGWI	ACGGA	2 cut(s)	21, 31
TspRI	CASTG	6 cut(s)	793, 1300, 1372, 1448, 1554, 2299
Vha464I	CTTAAG	2 cut(s)	25, 2164
VpaK11BI	GGWCC	1 cut(s)	1028
VspI	ATTAAT	1 cut(s)	378
XagI	CCTNNNNNAGG	2 cut(s)	182, 1164
XapI	RAATTY	8 cut(s)	243, 569, 616, 858, 998, 1135, 1558, 1687
XbaI	TCTAGA	2 cut(s)	874, 1679
XceI	RCATGY	4 cut(s)	441, 556, 591, 762
XhoI	CTCGAG	1 cut(s)	2001
XmaJI	CCTAGG	1 cut(s)	1871
XspI	CTAG	6 cut(s)	875, 936, 1205, 1680, 1872, 2012
Zsp2I	ATGCAT	3 cut(s)	308, 760, 764

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.