Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr5

Orthogroup ID

OG0013292

Physical Location & Seq

Genomic Coordinates Forward (+)

35597918 .. 35602998

Gene Structure 5081 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000024414

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2385 bp

ATGTACGGCGCGGCGGGGAAGAGCACGAGCTCGAGCTCGAGCTACGGCGCTTCATACGCGTCTGGGATCAGCGAGATCTGGGCCGCGTACTTGGCCGAAATCGACCTCGGCGACGACAAACCCTGGCTCAACCTCACACGCTCCCTCGTCAGCTTCTTCACTACGCCGCCGGGCCAAGCTCTTCTCTCTCAGGTCAAGGACGAGGACGGCGTGGTTGCGGTGTGGGTTGACTTTGAGAAATTTCGGAAAGAGTGTGAAGTTGAGGAGTTTTATGAAGCGTTGGAGGAGAAGCCGAAGGTAACCCTACTGTGCATGAGTATTGCAGTGCACAAGGTTCTGTTGACTCAATGGGAAAGTGATAGAATGGAGGATGATATCCGAGTTAATATTCGGTTGCATAACTACCCAGAATCGATGATTGCTTTGAAGAACTTAAAAGCTGCTTACATAGACAAGCTTGTATCTGTGCGCGGTAGTGTAGTAAAAGTTAGCACTGTGAGGCCTTTTGTGGTTCAAATGGATTTTGACTGTGTGAAGTGTAAGACGAGCATTACCCGTATGTTCCCCGATGGGAAGTTTTCCCCACCATTGAAATGTGATCTACAAGGGTGCAAGAGCAGAACTTTTATTCCAAATCGATCTACTGCTCAAACGATAGATTTTCAAAAGATAAGGATACAGGAGCTGCTGAAACCTGAGGATCATGAAGAAGGACGGGTGCCTCGAACAGTAGAATGTGAACTTCTTGAAGATCTTGTTGATGCATGCATCCCTGGAGATGTGGTGACTGTCACTGGGATAATAAGAGTCATTAACAATTACATGGACATTGGGGGAGGAAAATCGAAAGGCAAAAGTCAGGGAATTTACTATTTGTATCTAGAAGCTGTTTCAATTAAAAACTCCAAGTCACAGTCCATGCCTGAAGGTGTACAAGATTCTAGTTCTAATGCTGGGTCTGTAGCACTTGTTGACTCATTTTCCCCAAGGGATTTGGAATTCATTGTCAAGTTCTCGGAGGAACATGGTCCAGATACCTTCCGGCAACTAATCCAATCTATCTGTCCATCCATCTATGGCCATGAGCTCGTTAAAGCGGGGATAATACTGGCACTCTTTGGAGGTGTAAGGAAGCACTCAAATGATCAAAACAAGGTTCCTGTTAGAGGAGACATTCATGTCCTTATTGTTGGTGATCCTGGACTAGGAAAGAGCCAAATACTTCAAGCAGCAGCTGCTGTTTCTCCGCGAGGCATTTATGTATGTGGTAATGCCACAACCAGAGCTGGGCTCACTGTAGCTGTTGTAAAGGATTCTATGACAAGTGATTATGCGTTTGAAGCAGGTGCCATGGTACTTGCAGATAGTGGCTTGTGCTGCATTGATGAGTTCGACAAAATGACTTCAGAGCATCAGGCCCTACTGGAAGCCATGGAACAACAGTGTGTGTCTATTGCAAAGGCTGGATTAGTGGCAAGTCTGTCAGCACGAACTTCTGTCTTAGCAGCAGCAAACCCTGTTGGTGGTCATTATAATCGTGCCAAAACTGTGAATGAAAATCTGAAAATAAGTGCTGCTCTCCTCTCACGATTTGATTTGGTTTTCATATTACTTGACAAACCCGATGAACTTCTGGATAAGCGAGTCTCAGAGCACATTATGTCACTTCATACTGGATATGGAGAGCATTCGCCAGCTGCAAAAAAGATTCGTAGAGAAAATGCAGCATCACAAAGTGCAGAAGGCATAGATATGAGCGGAGGTCGTTCTTTGGTTTCAAGGTTGAGGCTTGACCCAAAGAAGGATGCTGATTTTGTTCCATTACCCATGCAACTTCTTCGCAAGTATATTGCTTATGCAAAAACGTTTGTCTTTCCAAGGATGTCAAAGCCGGCAGCAGAAATCCTGCAGAAATTTTACTTGAAACTGAGAGACCATGCTACATCTGCTGATAGCACACCAATAACAGCGAGGCAACTGGAGAGTCTGGTGAGGCTGACAGAAGCTCGAGCTAGGTTAGATTTAAGGGAGGAAATTACAGCCCAAGATGCAACGGAAGTTGTTGAAATCATGAGAGAATCCTTGTATGACAAGTACATCGATGAGCATGGTGTTGTGGATTTTGGTCGGAGTGGAGGGATGAGTCAACAAAAAGAAGCAAAGCGGTTTTTAAGTGCCCTTAATAAGCAATCAGAGTTGCACCAGAAAGACACTTTCTCAATTTCTGAAATATACAGTTTGGCAGATAGGATTGGCCTAAGAGTTCCAGACATTGACACATTCGTTGATAACTTAAACAGTGTTGGCTATCTACTCAAGAAGGGGGGGAAGACATACCAGCTACTATCATCATCTTATTCTAGGAGTCAATCATCATCAAGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000280 GO:0000724 GO:0000725 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006259 GO:0006281 GO:0006302 GO:0006310 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007049 GO:0007140 GO:0007143 GO:0007275 GO:0007276 GO:0007292 GO:0008150 GO:0008152 GO:0009555 GO:0009987 GO:0016043 GO:0019953 GO:0022402 GO:0022412 GO:0022414 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032504 GO:0032991 GO:0033554 GO:0034641 GO:0042555 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0044703 GO:0046483 GO:0048229 GO:0048232 GO:0048285 GO:0048609 GO:0048856 GO:0050896 GO:0051321 GO:0051704 GO:0051716 GO:0070013 GO:0071704 GO:0071840 GO:0090304 GO:0097362 GO:0140013 GO:1901360 GO:1903046

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MCM MCM_OB

Protein Analysis

795

Amino Acids

87.65

Weight (kDa)

6.36

Isoelectric Point (pI)

45.6

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MCM_OB	PF17207	142 - 271	8.7e-29	MCM OB domain
MCM	PF00493	343 - 557	9.9e-91	MCM P-loop domain
AAA_5	PF07728	393 - 531	2e-07	AAA domain (dynein-related subfamily)
MCM_lid	PF17855	612 - 695	3.9e-26	MCM AAA-lid domain
WHD_MCM8	PF25051	707 - 782	3.6e-30	MCM8 winged helix domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0013292)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G09660 AT3G09660 AT3G09660
fragaria_vesca	FvH4_6g15280 FvH4_6g15280
malus_domestica	MD04G1104500.v1.1
prunus_persica	Prupe.6G241500_v2.0.a1 Prupe.6G241500_v2.0.a1
pyrus_communis	pycom04g09950
rosa_chinensis	RchiOBHm_Chr3g0468711
rosa_laevigata	RLG00000024414
rosa_multiflora	Rmu_sc0011538.1_g000001
rosa_roxburghii	Rroxscaffold_6G00412640
rosa_rugosa	Rorug03G0096200
rosa_samantha	Rh3AG145200 Rh3BG167800 Rh3DG166500
rosa_wichuraiana	Rw3G013700

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1533
AarI	CACCTGC	1 cut(s)	1334
AasI	GACNNNNNNGTC	1 cut(s)	1178
Acc36I	ACCTGC	1 cut(s)	1334
AccB1I	GGYRCC	2 cut(s)	718, 1346
AccBSI	CCGCTC	1 cut(s)	1758
AccII	CGCG	5 cut(s)	11, 59, 86, 471, 1249
AclI	AACGTT	1 cut(s)	1865
AclWI	GGATC	3 cut(s)	74, 708, 1190
AcoI	YGGCCR	2 cut(s)	93, 1078
AcsI	RAATTY	4 cut(s)	239, 864, 998, 1913
AcuI	CTGAAG	2 cut(s)	945, 1389
AdeI	CACNNNGTG	1 cut(s)	1736
AfaI	GTAC	5 cut(s)	5, 89, 933, 1356, 2096
AfiI	CCNNNNNNNGG	5 cut(s)	1166, 1205, 1287, 1523, 1801
AflIII	ACRYGT	1 cut(s)	57
AjnI	CCWGG	3 cut(s)	122, 772, 1198
AloI	GAACNNNNNNTCC	2 cut(s)	613, 645
Alw21I	GWGCWC	6 cut(s)	26, 32, 38, 330, 1089, 1656
Alw26I	GTCTC	3 cut(s)	1164, 1651, 1926
Alw44I	GTGCAC	1 cut(s)	326
AlwI	GGATC	3 cut(s)	74, 708, 1190
AlwNI	CAGNNNCTG	3 cut(s)	685, 1235, 1238
Ama87I	CYCGRG	3 cut(s)	31, 37, 2007
AoxI	GGCC	7 cut(s)	81, 93, 172, 500, 1078, 1416, 2254
ApaLI	GTGCAC	1 cut(s)	326
ApoI	RAATTY	4 cut(s)	239, 864, 998, 1913
AspLEI	GCGC	3 cut(s)	11, 50, 471
AspS9I	GGNCC	4 cut(s)	81, 172, 1028, 1417
AsuC2I	CCSGG	1 cut(s)	171
AsuHPI	GGTGA	3 cut(s)	796, 1205, 2002
AvaI	CYCGRG	3 cut(s)	31, 37, 2007
AvaII	GGWCC	1 cut(s)	1028
AxyI	CCTNAGG	1 cut(s)	696
BaeGI	GKGCMC	2 cut(s)	330, 2179
BalI	TGGCCA	1 cut(s)	1080
BanI	GGYRCC	2 cut(s)	718, 1346
BanII	GRGCYC	4 cut(s)	32, 38, 1089, 1293
BauI	CACGAG	1 cut(s)	25
BbsI	GAAGAC	1 cut(s)	2336
Bbv12I	GWGCWC	6 cut(s)	26, 32, 38, 330, 1089, 1656
BccI	CCATC	3 cut(s)	563, 1075, 1079
BceAI	ACGGC	3 cut(s)	22, 61, 223
BcgI	CGANNNNNNTGC	2 cut(s)	1820, 1854
BciT130I	CCWGG	3 cut(s)	124, 774, 1200
BciVI	GTATCC	1 cut(s)	669
BclI	TGATCA	1 cut(s)	1144
BcnI	CCSGG	1 cut(s)	171
BcoDI	GTCTC	3 cut(s)	1164, 1651, 1926
BfaI	CTAG	5 cut(s)	881, 942, 1205, 2013, 2361
BfmI	CTRYAG	3 cut(s)	960, 1296, 1907
BfoI	RGCGCY	1 cut(s)	51
BfuAI	ACCTGC	1 cut(s)	1334
BfuI	GTATCC	1 cut(s)	669
BglII	AGATCT	2 cut(s)	75, 751
Bme1390I	CCNGG	4 cut(s)	124, 171, 774, 1200
Bme18I	GGWCC	1 cut(s)	1028
BmeT110I	CYCGRG	3 cut(s)	31, 37, 2007
BmgT120I	GGNCC	4 cut(s)	81, 172, 1028, 1417
BmiI	GGNNCC	3 cut(s)	720, 1158, 1348
BmrFI	CCNGG	4 cut(s)	124, 171, 774, 1200
BmrI	ACTGGG	1 cut(s)	804
BmsI	GCATC	6 cut(s)	751, 777, 1420, 1736, 1795, 2038
BmuI	ACTGGG	1 cut(s)	804
BpiI	GAAGAC	1 cut(s)	2336
BplI	GAGNNNNNCTC	2 cut(s)	1275, 1307
BpmI	CTGGAG	2 cut(s)	795, 2000
BpuEI	CTTGAG	1 cut(s)	2300
BpuMI	CCSGG	1 cut(s)	171
Bsa29I	ATCGAT	3 cut(s)	413, 637, 2100
BsaI	GGTCTC	1 cut(s)	1926
BsaJI	CCNNGG	7 cut(s)	106, 122, 772, 986, 1350, 1431, 1877
BsaXI	ACNNNNNCTCC	2 cut(s)	768, 798
Bsc4I	CCNNNNNNNGG	5 cut(s)	1166, 1205, 1287, 1523, 1801
Bse118I	RCCGGY	1 cut(s)	1891
Bse1I	ACTGG	5 cut(s)	799, 1113, 1428, 1678, 1983
Bse21I	CCTNAGG	1 cut(s)	696
BseBI	CCWGG	3 cut(s)	124, 774, 1200
BseCI	ATCGAT	3 cut(s)	413, 637, 2100
BseDI	CCNNGG	7 cut(s)	106, 122, 772, 986, 1350, 1431, 1877
BseGI	GGATG	6 cut(s)	376, 768, 1067, 1810, 1887, 2145
BseLI	CCNNNNNNNGG	5 cut(s)	1166, 1205, 1287, 1523, 1801
BseMII	CTCAG	4 cut(s)	203, 687, 1662, 1919
BseNI	ACTGG	5 cut(s)	799, 1113, 1428, 1678, 1983
BseRI	GAGGAG	4 cut(s)	278, 299, 1182, 1571
BseSI	GKGCMC	2 cut(s)	330, 2179
BseYI	CCCAGC	2 cut(s)	953, 1286
BsgI	GTGCAG	1 cut(s)	1758
Bsh1236I	CGCG	5 cut(s)	11, 59, 86, 471, 1249
BshFI	GGCC	7 cut(s)	83, 95, 174, 502, 1080, 1418, 2256
BshNI	GGYRCC	2 cut(s)	718, 1346
BshVI	ATCGAT	3 cut(s)	413, 637, 2100
BsiHKAI	GWGCWC	6 cut(s)	26, 32, 38, 330, 1089, 1656
BsiHKCI	CYCGRG	3 cut(s)	31, 37, 2007
BsiSI	CCGG	3 cut(s)	170, 1042, 1892
BslI	CCNNNNNNNGG	5 cut(s)	1166, 1205, 1287, 1523, 1801
BsmAI	GTCTC	3 cut(s)	1164, 1651, 1926
BsmI	GAATGC	1 cut(s)	1687
BsnI	GGCC	7 cut(s)	83, 95, 174, 502, 1080, 1418, 2256
Bso31I	GGTCTC	1 cut(s)	1926
BsoBI	CYCGRG	3 cut(s)	31, 37, 2007
Bsp1286I	GDGCHC	8 cut(s)	26, 32, 38, 330, 1089, 1293, 1656, 2179
Bsp1407I	TGTACA	1 cut(s)	931
Bsp143I	GATC	8 cut(s)	66, 75, 598, 638, 700, 751, 1144, 1195
Bsp19I	CCATGG	2 cut(s)	1350, 1431
BspANI	GGCC	7 cut(s)	83, 95, 174, 502, 1080, 1418, 2256
BspCNI	CTCAG	4 cut(s)	202, 688, 1661, 1920
BspDI	ATCGAT	3 cut(s)	413, 637, 2100
BspFNI	CGCG	5 cut(s)	11, 59, 86, 471, 1249
BspHI	TCATGA	2 cut(s)	703, 2070
BspLI	GGNNCC	3 cut(s)	720, 1158, 1348
BspMAI	CTGCAG	1 cut(s)	1911
BspMI	ACCTGC	1 cut(s)	1334
BspPI	GGATC	3 cut(s)	74, 708, 1190
BspQI	GCTCTTC	2 cut(s)	14, 186
BspT107I	GGYRCC	2 cut(s)	718, 1346
BspTNI	GGTCTC	1 cut(s)	1926
BsrBI	CCGCTC	1 cut(s)	1758
BsrFI	RCCGGY	1 cut(s)	1891
BsrGI	TGTACA	1 cut(s)	931
BsrI	ACTGG	5 cut(s)	799, 1113, 1428, 1678, 1983
BssAI	RCCGGY	1 cut(s)	1891
BssECI	CCNNGG	7 cut(s)	106, 122, 772, 986, 1350, 1431, 1877
BssMI	GATC	8 cut(s)	66, 75, 598, 638, 700, 751, 1144, 1195
BssSI	CACGAG	1 cut(s)	25
BssT1I	CCWWGG	4 cut(s)	986, 1350, 1431, 1877
Bst2BI	CACGAG	1 cut(s)	25
Bst2UI	CCWGG	3 cut(s)	124, 774, 1200
Bst6I	CTCTTC	2 cut(s)	14, 186
BstAPI	GCANNNNNTGC	1 cut(s)	1235
BstAUI	TGTACA	1 cut(s)	931
BstC8I	GCNNGC	3 cut(s)	766, 1695, 1893
BstDEI	CTNAG	6 cut(s)	189, 696, 1501, 1648, 1928, 2258
BstDSI	CCRYGG	2 cut(s)	1350, 1431
BstEII	GGTNACC	1 cut(s)	298
BstENI	CCTNNNNNAGG	2 cut(s)	1164, 1203
BstF5I	GGATG	6 cut(s)	376, 768, 1067, 1810, 1887, 2145
BstFNI	CGCG	5 cut(s)	11, 59, 86, 471, 1249
BstH2I	RGCGCY	1 cut(s)	51
BstHHI	GCGC	3 cut(s)	11, 50, 471
BstKTI	GATC	8 cut(s)	69, 78, 601, 641, 703, 754, 1147, 1198
BstMAI	GTCTC	3 cut(s)	1164, 1651, 1926
BstMBI	GATC	8 cut(s)	66, 75, 598, 638, 700, 751, 1144, 1195
BstMWI	GCNNNNNNNGC	7 cut(s)	56, 92, 1235, 1340, 1377, 1946, 2048
BstNI	CCWGG	3 cut(s)	124, 774, 1200
BstNSI	RCATGY	1 cut(s)	768
BstPI	GGTNACC	1 cut(s)	298
BstSCI	CCNGG	4 cut(s)	122, 169, 772, 1198
BstSFI	CTRYAG	3 cut(s)	960, 1296, 1907
BstSLI	GKGCMC	2 cut(s)	330, 2179
BstUI	CGCG	5 cut(s)	11, 59, 86, 471, 1249
BstV2I	GAAGAC	1 cut(s)	2336
BstX2I	RGATCY	2 cut(s)	75, 751
BstYI	RGATCY	2 cut(s)	75, 751
Bsu15I	ATCGAT	3 cut(s)	413, 637, 2100
Bsu36I	CCTNAGG	1 cut(s)	696
BsuI	GTATCC	1 cut(s)	669
BsuRI	GGCC	7 cut(s)	83, 95, 174, 502, 1080, 1418, 2256
BsuTUI	ATCGAT	3 cut(s)	413, 637, 2100
BtgI	CCRYGG	2 cut(s)	1350, 1431
BtsCI	GGATG	6 cut(s)	376, 768, 1067, 1810, 1887, 2145
BtsI	GCAGTG	1 cut(s)	330
BtsIMutI	CAGTG	6 cut(s)	330, 492, 792, 1293, 1448, 2305
BveI	ACCTGC	1 cut(s)	1334
Cac8I	GCNNGC	3 cut(s)	766, 1695, 1893
CaiI	CAGNNNCTG	3 cut(s)	685, 1235, 1238
CciI	TCATGA	2 cut(s)	703, 2070
CfoI	GCGC	3 cut(s)	11, 50, 471
Cfr10I	RCCGGY	1 cut(s)	1891
Cfr13I	GGNCC	4 cut(s)	81, 172, 1028, 1417
ClaI	ATCGAT	3 cut(s)	413, 637, 2100
CseI	GACGC	1 cut(s)	48
Csp6I	GTAC	5 cut(s)	4, 88, 932, 1355, 2095
CviQI	GTAC	5 cut(s)	4, 88, 932, 1355, 2095
DdeI	CTNAG	6 cut(s)	189, 696, 1501, 1648, 1928, 2258
DpnI	GATC	8 cut(s)	68, 77, 600, 640, 702, 753, 1146, 1197
DpnII	GATC	8 cut(s)	66, 75, 598, 638, 700, 751, 1144, 1195
DraIII	CACNNNGTG	1 cut(s)	1736
DrdI	GACNNNNNNGTC	1 cut(s)	1178
DseDI	GACNNNNNNGTC	1 cut(s)	1178
EaeI	YGGCCR	2 cut(s)	93, 1078
Eam1104I	CTCTTC	2 cut(s)	14, 186
EarI	CTCTTC	2 cut(s)	14, 186
Ecl136II	GAGCTC	3 cut(s)	30, 36, 1087
Eco130I	CCWWGG	4 cut(s)	986, 1350, 1431, 1877
Eco147I	AGGCCT	1 cut(s)	502
Eco24I	GRGCYC	4 cut(s)	32, 38, 1089, 1293
Eco31I	GGTCTC	1 cut(s)	1926
Eco32I	GATATC	1 cut(s)	376
Eco47I	GGWCC	1 cut(s)	1028
Eco53kI	GAGCTC	3 cut(s)	30, 36, 1087
Eco57I	CTGAAG	2 cut(s)	945, 1389
Eco81I	CCTNAGG	1 cut(s)	696
Eco88I	CYCGRG	3 cut(s)	31, 37, 2007
Eco91I	GGTNACC	1 cut(s)	298
EcoICRI	GAGCTC	3 cut(s)	30, 36, 1087
EcoNI	CCTNNNNNAGG	2 cut(s)	1164, 1203
EcoO109I	RGGNCCY	1 cut(s)	1417
EcoO65I	GGTNACC	1 cut(s)	298
EcoRI	GAATTC	1 cut(s)	998
EcoRII	CCWGG	3 cut(s)	122, 772, 1198
EcoRV	GATATC	1 cut(s)	376
EcoT14I	CCWWGG	4 cut(s)	986, 1350, 1431, 1877
EcoT22I	ATGCAT	2 cut(s)	766, 770
EcoT38I	GRGCYC	4 cut(s)	32, 38, 1089, 1293
ErhI	CCWWGG	4 cut(s)	986, 1350, 1431, 1877
FauI	CCCGC	2 cut(s)	7, 1090
FbaI	TGATCA	1 cut(s)	1144
FokI	GGATG	6 cut(s)	383, 755, 1054, 1817, 1894, 2152
FriOI	GRGCYC	4 cut(s)	32, 38, 1089, 1293
FspBI	CTAG	5 cut(s)	881, 942, 1205, 2013, 2361
GlaI	GCGC	3 cut(s)	10, 49, 470
GsaI	CCCAGC	2 cut(s)	957, 1290
GsuI	CTGGAG	2 cut(s)	795, 2000
HaeII	RGCGCY	1 cut(s)	51
HaeIII	GGCC	7 cut(s)	83, 95, 174, 502, 1080, 1418, 2256
HapII	CCGG	3 cut(s)	170, 1042, 1892
HgaI	GACGC	1 cut(s)	48
HhaI	GCGC	3 cut(s)	11, 50, 471
Hin6I	GCGC	3 cut(s)	9, 48, 469
HinP1I	GCGC	3 cut(s)	9, 48, 469
HincII	GTYRAC	4 cut(s)	229, 342, 973, 2147
HindII	GTYRAC	4 cut(s)	229, 342, 973, 2147
HindIII	AAGCTT	1 cut(s)	455
HpaII	CCGG	3 cut(s)	170, 1042, 1892
HphI	GGTGA	3 cut(s)	796, 1205, 2002
Hpy166II	GTNNAC	7 cut(s)	229, 328, 342, 740, 932, 973, 2147
Hpy188I	TCNGA	9 cut(s)	246, 380, 1018, 1408, 1563, 1651, 2130, 2194, 2227
Hpy188III	TCNNGA	9 cut(s)	704, 746, 881, 1031, 1587, 1634, 2071, 2267, 2317
Hpy8I	GTNNAC	7 cut(s)	229, 328, 342, 740, 932, 973, 2147
Hpy99I	CGWCG	1 cut(s)	116
HpyAV	CCTTC	7 cut(s)	289, 704, 920, 1048, 1736, 1795, 2314
HpyCH4IV	ACGT	1 cut(s)	1865
HpyF10VI	GCNNNNNNNGC	7 cut(s)	56, 92, 1235, 1340, 1377, 1946, 2048
HpyF3I	CTNAG	6 cut(s)	189, 696, 1501, 1648, 1928, 2258
HpySE526I	ACGT	1 cut(s)	1865
HspAI	GCGC	3 cut(s)	9, 48, 469
KroI	GCCGGC	1 cut(s)	1891
KroNI	GCCGGC	1 cut(s)	1893
Ksp22I	TGATCA	1 cut(s)	1144
Kzo9I	GATC	8 cut(s)	66, 75, 598, 638, 700, 751, 1144, 1195
LguI	GCTCTTC	2 cut(s)	14, 186
LmnI	GCTCC	2 cut(s)	146, 682
LweI	GCATC	6 cut(s)	751, 777, 1420, 1736, 1795, 2038
MaeI	CTAG	5 cut(s)	881, 942, 1205, 2013, 2361
MaeII	ACGT	1 cut(s)	1865
MaeIII	GTNAC	5 cut(s)	298, 784, 790, 909, 1662
MalI	GATC	8 cut(s)	68, 77, 600, 640, 702, 753, 1146, 1197
MbiI	CCGCTC	1 cut(s)	1758
MboI	GATC	8 cut(s)	66, 75, 598, 638, 700, 751, 1144, 1195
MboII	GAAGA	8 cut(s)	31, 148, 173, 439, 719, 761, 1829, 2341
MflI	RGATCY	2 cut(s)	75, 751
MhlI	GDGCHC	8 cut(s)	26, 32, 38, 330, 1089, 1293, 1656, 2179
MlsI	TGGCCA	1 cut(s)	1080
MluCI	AATT	8 cut(s)	239, 817, 864, 894, 998, 1913, 2034, 2220
MluI	ACGCGT	1 cut(s)	57
MluNI	TGGCCA	1 cut(s)	1080
MlyI	GAGTC	7 cut(s)	337, 816, 968, 1653, 1993, 2152, 2374
MmeI	TCCRAC	2 cut(s)	261, 2108
Mox20I	TGGCCA	1 cut(s)	1080
Mph1103I	ATGCAT	2 cut(s)	766, 770
MroNI	GCCGGC	1 cut(s)	1891
MscI	TGGCCA	1 cut(s)	1080
MseI	TTAA	9 cut(s)	384, 434, 813, 897, 1092, 2024, 2171, 2181, 2294
MslI	CAYNNNNRTG	4 cut(s)	592, 1140, 1751, 2380
Msp20I	TGGCCA	1 cut(s)	1080
MspA1I	CMGCKG	2 cut(s)	1235, 1697
MspI	CCGG	3 cut(s)	170, 1042, 1892
MspR9I	CCNGG	4 cut(s)	124, 171, 774, 1200
Mva1269I	GAATGC	1 cut(s)	1687
MvaI	CCWGG	3 cut(s)	124, 774, 1200
MvnI	CGCG	5 cut(s)	11, 59, 86, 471, 1249
MwoI	GCNNNNNNNGC	7 cut(s)	56, 92, 1235, 1340, 1377, 1946, 2048
NaeI	GCCGGC	1 cut(s)	1893
NciI	CCSGG	1 cut(s)	171
NcoI	CCATGG	2 cut(s)	1350, 1431
NdeII	GATC	8 cut(s)	66, 75, 598, 638, 700, 751, 1144, 1195
NgoMIV	GCCGGC	1 cut(s)	1891
NlaIV	GGNNCC	3 cut(s)	720, 1158, 1348
NmeAIII	GCCGAG	1 cut(s)	87
NmuCI	GTSAC	4 cut(s)	784, 790, 909, 1662
NsiI	ATGCAT	2 cut(s)	766, 770
NspI	RCATGY	1 cut(s)	768
PaeI	GCATGC	1 cut(s)	768
PaeR7I	CTCGAG	3 cut(s)	31, 37, 2007
PagI	TCATGA	2 cut(s)	703, 2070
PaqCI	CACCTGC	1 cut(s)	1334
PceI	AGGCCT	1 cut(s)	502
PciSI	GCTCTTC	2 cut(s)	14, 186
PcsI	WCGNNNNNNNCGW	3 cut(s)	108, 207, 721
PctI	GAATGC	1 cut(s)	1687
PdiI	GCCGGC	1 cut(s)	1893
PfeI	GAWTC	5 cut(s)	410, 938, 1313, 1708, 2078
PfoI	TCCNGGA	1 cut(s)	1198
PleI	GAGTC	7 cut(s)	337, 815, 968, 1652, 1992, 2151, 2373
PpsI	GAGTC	7 cut(s)	337, 815, 968, 1652, 1992, 2151, 2373
PsiI	TTATAA	1 cut(s)	1533
Psp124BI	GAGCTC	3 cut(s)	32, 38, 1089
Psp1406I	AACGTT	1 cut(s)	1865
Psp6I	CCWGG	3 cut(s)	122, 772, 1198
PspEI	GGTNACC	1 cut(s)	298
PspFI	CCCAGC	2 cut(s)	953, 1286
PspGI	CCWGG	3 cut(s)	122, 772, 1198
PspN4I	GGNNCC	3 cut(s)	720, 1158, 1348
PspPI	GGNCC	4 cut(s)	81, 172, 1028, 1417
PspXI	VCTCGAGB	3 cut(s)	31, 37, 2007
PstI	CTGCAG	1 cut(s)	1911
PstNI	CAGNNNCTG	3 cut(s)	685, 1235, 1238
PsuI	RGATCY	2 cut(s)	75, 751
PvuII	CAGCTG	2 cut(s)	1235, 1697
RsaI	GTAC	5 cut(s)	5, 89, 933, 1356, 2096
RsaNI	GTAC	5 cut(s)	4, 88, 932, 1355, 2095
RseI	CAYNNNNRTG	4 cut(s)	592, 1140, 1751, 2380
SacI	GAGCTC	3 cut(s)	32, 38, 1089
SapI	GCTCTTC	2 cut(s)	14, 186
SaqAI	TTAA	9 cut(s)	384, 434, 813, 897, 1092, 2024, 2171, 2181, 2294
Sau3AI	GATC	8 cut(s)	66, 75, 598, 638, 700, 751, 1144, 1195
Sau96I	GGNCC	4 cut(s)	81, 172, 1028, 1417
SchI	GAGTC	7 cut(s)	337, 816, 968, 1653, 1993, 2152, 2374
ScrFI	CCNGG	4 cut(s)	124, 171, 774, 1200
SduI	GDGCHC	8 cut(s)	26, 32, 38, 330, 1089, 1293, 1656, 2179
SfaNI	GCATC	6 cut(s)	751, 777, 1420, 1736, 1795, 2038
SfcI	CTRYAG	3 cut(s)	960, 1296, 1907
Sfr274I	CTCGAG	3 cut(s)	31, 37, 2007
SinI	GGWCC	1 cut(s)	1028
SlaI	CTCGAG	3 cut(s)	31, 37, 2007
SmiMI	CAYNNNNRTG	4 cut(s)	592, 1140, 1751, 2380
SmlI	CTYRAG	4 cut(s)	31, 37, 2007, 2315
SmoI	CTYRAG	4 cut(s)	31, 37, 2007, 2315
SphI	GCATGC	1 cut(s)	768
Sse9I	AATT	8 cut(s)	239, 817, 864, 894, 998, 1913, 2034, 2220
SseBI	AGGCCT	1 cut(s)	502
SspI	AATATT	1 cut(s)	388
SspMI	CTAG	5 cut(s)	881, 942, 1205, 2013, 2361
SstI	GAGCTC	3 cut(s)	32, 38, 1089
StuI	AGGCCT	1 cut(s)	502
StyD4I	CCNGG	4 cut(s)	122, 169, 772, 1198
StyI	CCWWGG	4 cut(s)	986, 1350, 1431, 1877
TaiI	ACGT	1 cut(s)	1868
TasI	AATT	8 cut(s)	239, 817, 864, 894, 998, 1913, 2034, 2220
TatI	WGTACW	2 cut(s)	931, 2094
TauI	GCSGC	3 cut(s)	14, 86, 169
TfiI	GAWTC	5 cut(s)	410, 938, 1313, 1708, 2078
Tru1I	TTAA	9 cut(s)	384, 434, 813, 897, 1092, 2024, 2171, 2181, 2294
Tru9I	TTAA	9 cut(s)	384, 434, 813, 897, 1092, 2024, 2171, 2181, 2294
TscAI	CASTG	6 cut(s)	330, 499, 799, 1300, 1448, 2305
TseFI	GTSAC	4 cut(s)	784, 790, 909, 1662
Tsp45I	GTSAC	4 cut(s)	784, 790, 909, 1662
TspDTI	ATGAA	9 cut(s)	42, 288, 720, 991, 1166, 1569, 1594, 1641, 1658
TspGWI	ACGGA	1 cut(s)	2069
TspRI	CASTG	6 cut(s)	330, 499, 799, 1300, 1448, 2305
VneI	GTGCAC	1 cut(s)	326
VpaK11BI	GGWCC	1 cut(s)	1028
XagI	CCTNNNNNAGG	2 cut(s)	1164, 1203
XapI	RAATTY	4 cut(s)	239, 864, 998, 1913
XbaI	TCTAGA	1 cut(s)	880
XceI	RCATGY	1 cut(s)	768
XhoI	CTCGAG	3 cut(s)	31, 37, 2007
XspI	CTAG	5 cut(s)	881, 942, 1205, 2013, 2361
Zsp2I	ATGCAT	2 cut(s)	766, 770

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.