Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000006

Orthogroup ID

OG0013292

Physical Location & Seq

Genomic Coordinates Forward (+)

35135260 .. 35141284

Gene Structure 6025 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_6G00412640.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2355 bp

ATGTACGGCGCGGCGGGGAAGAGCACGAGCTCGAGCTACGGCGCTTCATACGCGTCTGGGCTCAGCGAGATCTGGGCCGCGTACTTGGCCCAAATCGAGCTCGGCGACGACAAACCCTGGCTCAACCTCACACGCTCCCTCGTCAGCTTCTTCACTACGCCGCCGGGCCAAGCTCTTCTCTCTCAGGTCAAGGACGAGGACGGCGTGGTTGCTGTGTGGGTTAACTTTGAGAAATTTCGGAAAGAGTGTGAAGTTGAGGAGTTTTATGAAGCGTTGGAGGAGAAACCGAAGGTAACCCTACTCTGCATGAGTATTGCAGTGCACAAGGTTCTGTTGACTCAATGGGAAAGTGATAGAATGGAGGATGATATCCGAGTTAATATTCGGTTGCATAACTACCCGGAATCGATGATTGCTTTGAAGAACTTAAAAGCTGCATATATAGACAAGCTTGTATCTGTGCGTGGTAGTGTAGTAAAAGTTAGCACTGTGAGGCCTTTTGTGGTGCAAATGGATTTTGACTGTGTGAAGTGTAAGACGAGCATTACGCGTATGTTCCCTGATGGGAAGTTTTCCCCACCATTGAAATGTGATCTTCATGGGTGCAAGAGCAGAACTTTTATTCCAAATCGATCTACTGCTCAAACGATAGATTTTCAAAAGATAAGGATACAGGAGCTACTGAAGCCTGAGGATCATGAAGAAGGCCGGGTGCCTCGAACAGTAGAATGTGAACTTCTTGAAGATCTTGTTGATGCATGCATCCCTGGAGATGTGGTGACTGTCACTGGGATAATAAGAGTCATTAACAATTACATGGACATTGGGGGAGGAAAATCGAAAGGCAAAAGTCAGGGAATTTACTATTTGTATCTAGAAGCTGTTTCAATTAAAAACTCCAAGTCACAGTCTATGCCTGAAGGTGTACAAGATTCTAGTTCTAATGCTGGGTCTGTAGCACTTGTTGACTCATTTTCCCCAAGGGATTTGGAATTCATTGTCAAGTTCTCAGAGGAACATGGTCCAGATACCTTCCGGCAACTAATCCAATCTATCTGTCCATCCATCTATGGCCATGAGCTCGTTAAAGCGGGGATAATACTGGCACTCTTTGGAGGTGTAAGGAAGCACTCAAATGATCAAAACAAGGTTCCTGTCAGAGGAGACATTCATGTCATTATTGTTGGTGATCCTGGACTAGGAAAGAGCCAAATACTTCAAGCAGCAGCAGCTGTTTCTCCGCGAGGCATTTATGTATGTGGTAATGCCACAACCAGAGCTGGGCTCACTGTAGCTGTTGTAAAGGATTCTATGACAAGTGATTATGCGTTTGAAGCAGGTGCCATGGTACTTGCAGATAGTGGCTTGTGCTGCATTGATGAGTTCGACAAAATGACTTCAGAGCATCAGGCCCTACTGGAAGCCATGGAACAACAGTGTGTGTCTATTGCAAAGGCTGGATTAGTGGCAAGTCTGTCAGCACGAACTTCTGTCTTAGCAGCAGCAAACCCTGTTGGTGGTCATTATAATCGTGCCAAAACTGTGAATGAAAATCTGAAAATAAGTGCTGCTCTCCTCTCACGATTTGATTTGGTTTTCATATTACTTGACAAACCCGATGAACTTCTGGATAAGCGAGTCTCAGAGCACATTATGTCACTTCATACTGGATATGGAGAGCATTCGCCAGCTGCAAAAAAGATTCGTAGAGAAAATGCAGCATCACAAAGTGCAGAAGGCATAGATATGAGCGGAGGTCGTTCTTTGGTTTCAAGGTTGAGGCTTGACCCAAAGAAGGATGCTGATTTTGTTCCATTACCCATGCAACTTCTTCGCAAGTACATTGCTTATGCAAAAACGTTTGTCTTTCCAAGGATGTCAAGGCCGGCAGCAGAAATCCTGCAGAAATTTTACTTGAAACTGAGAGACCATGCTACATCTGCTGATAGCACACCAATAACAGCGAGGCAACTGGAGAGTCTGGTGAGGCTGACAGAAGCTCGAGCTAGGTTAGATTTAAGGGAGGAAATTACAGCCCAAGATGCAACGGAAGTGGTTGAAATCATGAGAGAATCCTTGTATGACAAATACATCGATGAGCATGGTGTTGTGGATTTTGGTAGGAGTGGAGGGATGAGTCAACAAAAAGAAGCAAAGCGGTTTTTAAGTGCCCTTAATAAGCAATCAGAGTTGCACCAGAAAGACACTTTCTCAATTTCTGAAATATACAGTTTGGCAGATAGGATTGGCCTAAGAGTTCCAGACATTGACACATTCGTTGATAACTTAAACAGTGTGAAGAGGCTGAGTGAACTTGGTCTTATTTTTCACCTTCCTTTTTCGCTGCTACCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000280 GO:0000724 GO:0000725 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006259 GO:0006281 GO:0006302 GO:0006310 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007049 GO:0007140 GO:0007143 GO:0007275 GO:0007276 GO:0007292 GO:0008150 GO:0008152 GO:0009555 GO:0009987 GO:0016043 GO:0019953 GO:0022402 GO:0022412 GO:0022414 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032504 GO:0032991 GO:0033554 GO:0034641 GO:0042555 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0044703 GO:0046483 GO:0048229 GO:0048232 GO:0048285 GO:0048609 GO:0048856 GO:0050896 GO:0051321 GO:0051704 GO:0051716 GO:0070013 GO:0071704 GO:0071840 GO:0090304 GO:0097362 GO:0140013 GO:1901360 GO:1903046

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MCM MCM_OB

Protein Analysis

784

Amino Acids

86.72

Weight (kDa)

6.24

Isoelectric Point (pI)

45.02

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MCM_OB	PF17207	140 - 269	1.1e-28	MCM OB domain
MCM	PF00493	341 - 555	2.3e-90	MCM P-loop domain
AAA_5	PF07728	391 - 529	2e-07	AAA domain (dynein-related subfamily)
MCM_lid	PF17855	610 - 693	9.2e-26	MCM AAA-lid domain
WHD_MCM8	PF25051	705 - 765	3.1e-21	MCM8 winged helix domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0013292)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G09660 AT3G09660 AT3G09660
fragaria_vesca	FvH4_6g15280 FvH4_6g15280
malus_domestica	MD04G1104500.v1.1
prunus_persica	Prupe.6G241500_v2.0.a1 Prupe.6G241500_v2.0.a1
pyrus_communis	pycom04g09950
rosa_chinensis	RchiOBHm_Chr3g0468711
rosa_laevigata	RLG00000024414
rosa_multiflora	Rmu_sc0011538.1_g000001
rosa_roxburghii	Rroxscaffold_6G00412640
rosa_rugosa	Rorug03G0096200
rosa_samantha	Rh3AG145200 Rh3BG167800 Rh3DG166500
rosa_wichuraiana	Rw3G013700

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1527
AarI	CACCTGC	1 cut(s)	1328
AasI	GACNNNNNNGTC	1 cut(s)	1172
Acc36I	ACCTGC	1 cut(s)	1328
AccB1I	GGYRCC	2 cut(s)	712, 1340
AccBSI	CCGCTC	1 cut(s)	1752
AccII	CGCG	5 cut(s)	11, 53, 80, 550, 1243
AciI	CCGC	8 cut(s)	11, 14, 78, 161, 1091, 1241, 1752, 2158
AclI	AACGTT	1 cut(s)	1859
AclWI	GGATC	2 cut(s)	702, 1184
AcoI	YGGCCR	1 cut(s)	1072
AcsI	RAATTY	4 cut(s)	233, 858, 992, 1907
AcuI	CTGAAG	3 cut(s)	704, 939, 1383
AdeI	CACNNNGTG	1 cut(s)	1730
AfaI	GTAC	5 cut(s)	5, 83, 927, 1350, 1841
AfiI	CCNNNNNNNGG	5 cut(s)	1160, 1199, 1281, 1517, 1795
AflIII	ACRYGT	2 cut(s)	51, 548
AjnI	CCWGG	3 cut(s)	116, 766, 1192
AloI	GAACNNNNNNTCC	2 cut(s)	607, 639
Alw21I	GWGCWC	6 cut(s)	26, 32, 102, 324, 1083, 1650
Alw26I	GTCTC	3 cut(s)	1158, 1645, 1920
Alw44I	GTGCAC	1 cut(s)	320
AlwI	GGATC	2 cut(s)	702, 1184
AlwNI	CAGNNNCTG	1 cut(s)	1232
Ama87I	CYCGRG	2 cut(s)	31, 2001
AoxI	GGCC	9 cut(s)	75, 87, 166, 494, 706, 1072, 1410, 1883, 2248
ApaLI	GTGCAC	1 cut(s)	320
ApoI	RAATTY	4 cut(s)	233, 858, 992, 1907
AspLEI	GCGC	2 cut(s)	11, 44
AspS9I	GGNCC	5 cut(s)	75, 88, 166, 1022, 1411
AsuC2I	CCSGG	3 cut(s)	165, 401, 710
AsuHPI	GGTGA	4 cut(s)	790, 1199, 1996, 2321
AvaI	CYCGRG	2 cut(s)	31, 2001
AvaII	GGWCC	1 cut(s)	1022
AxyI	CCTNAGG	1 cut(s)	690
BaeGI	GKGCMC	2 cut(s)	324, 2173
BalI	TGGCCA	1 cut(s)	1074
BanI	GGYRCC	2 cut(s)	712, 1340
BanII	GRGCYC	5 cut(s)	32, 63, 102, 1083, 1287
BauI	CACGAG	1 cut(s)	25
Bbv12I	GWGCWC	6 cut(s)	26, 32, 102, 324, 1083, 1650
BccI	CCATC	3 cut(s)	557, 1069, 1073
BceAI	ACGGC	3 cut(s)	22, 55, 217
BcgI	CGANNNNNNTGC	2 cut(s)	1814, 1848
BciT130I	CCWGG	3 cut(s)	118, 768, 1194
BciVI	GTATCC	1 cut(s)	663
BclI	TGATCA	1 cut(s)	1138
BcnI	CCSGG	3 cut(s)	165, 401, 710
BcoDI	GTCTC	3 cut(s)	1158, 1645, 1920
BfaI	CTAG	4 cut(s)	875, 936, 1199, 2007
BfmI	CTRYAG	3 cut(s)	954, 1290, 1901
BfoI	RGCGCY	1 cut(s)	45
BfuAI	ACCTGC	1 cut(s)	1328
BfuI	GTATCC	1 cut(s)	663
BglII	AGATCT	2 cut(s)	69, 745
BlpI	GCTNAGC	1 cut(s)	62
Bme1390I	CCNGG	6 cut(s)	118, 165, 401, 710, 768, 1194
Bme18I	GGWCC	1 cut(s)	1022
BmeT110I	CYCGRG	2 cut(s)	31, 2001
BmgT120I	GGNCC	5 cut(s)	75, 88, 166, 1022, 1411
BmiI	GGNNCC	3 cut(s)	714, 1152, 1342
BmrFI	CCNGG	6 cut(s)	118, 165, 401, 710, 768, 1194
BmrI	ACTGGG	1 cut(s)	798
BmsI	GCATC	6 cut(s)	745, 771, 1414, 1730, 1789, 2032
BmuI	ACTGGG	1 cut(s)	798
BplI	GAGNNNNNCTC	2 cut(s)	1269, 1301
BpmI	CTGGAG	2 cut(s)	789, 1994
Bpu1102I	GCTNAGC	1 cut(s)	62
BpuMI	CCSGG	3 cut(s)	165, 401, 710
Bsa29I	ATCGAT	3 cut(s)	407, 631, 2094
BsaI	GGTCTC	1 cut(s)	1920
BsaJI	CCNNGG	6 cut(s)	116, 766, 980, 1344, 1425, 1871
BsaXI	ACNNNNNCTCC	2 cut(s)	762, 792
Bsc4I	CCNNNNNNNGG	5 cut(s)	1160, 1199, 1281, 1517, 1795
Bse118I	RCCGGY	1 cut(s)	1885
Bse1I	ACTGG	5 cut(s)	793, 1107, 1422, 1672, 1977
Bse21I	CCTNAGG	1 cut(s)	690
Bse3DI	GCAATG	1 cut(s)	1842
BseBI	CCWGG	3 cut(s)	118, 768, 1194
BseCI	ATCGAT	3 cut(s)	407, 631, 2094
BseDI	CCNNGG	6 cut(s)	116, 766, 980, 1344, 1425, 1871
BseGI	GGATG	6 cut(s)	370, 762, 1061, 1804, 1881, 2139
BseLI	CCNNNNNNNGG	5 cut(s)	1160, 1199, 1281, 1517, 1795
BseMI	GCAATG	1 cut(s)	1842
BseMII	CTCAG	7 cut(s)	76, 197, 681, 1023, 1656, 1913, 2297
BseNI	ACTGG	5 cut(s)	793, 1107, 1422, 1672, 1977
BseRI	GAGGAG	4 cut(s)	272, 293, 1176, 1565
BseSI	GKGCMC	2 cut(s)	324, 2173
BseYI	CCCAGC	2 cut(s)	947, 1280
BsgI	GTGCAG	1 cut(s)	1752
Bsh1236I	CGCG	5 cut(s)	11, 53, 80, 550, 1243
BshFI	GGCC	9 cut(s)	77, 89, 168, 496, 708, 1074, 1412, 1885, 2250
BshNI	GGYRCC	2 cut(s)	712, 1340
BshVI	ATCGAT	3 cut(s)	407, 631, 2094
BsiHKAI	GWGCWC	6 cut(s)	26, 32, 102, 324, 1083, 1650
BsiHKCI	CYCGRG	2 cut(s)	31, 2001
BsiSI	CCGG	5 cut(s)	164, 401, 709, 1036, 1886
BslI	CCNNNNNNNGG	5 cut(s)	1160, 1199, 1281, 1517, 1795
BsmAI	GTCTC	3 cut(s)	1158, 1645, 1920
BsmI	GAATGC	1 cut(s)	1681
BsnI	GGCC	9 cut(s)	77, 89, 168, 496, 708, 1074, 1412, 1885, 2250
Bso31I	GGTCTC	1 cut(s)	1920
BsoBI	CYCGRG	2 cut(s)	31, 2001
Bsp1286I	GDGCHC	9 cut(s)	26, 32, 63, 102, 324, 1083, 1287, 1650, 2173
Bsp1407I	TGTACA	1 cut(s)	925
Bsp143I	GATC	7 cut(s)	69, 592, 632, 694, 745, 1138, 1189
Bsp1720I	GCTNAGC	1 cut(s)	62
Bsp19I	CCATGG	2 cut(s)	1344, 1425
BspACI	CCGC	8 cut(s)	11, 14, 78, 161, 1091, 1241, 1752, 2158
BspANI	GGCC	9 cut(s)	77, 89, 168, 496, 708, 1074, 1412, 1885, 2250
BspCNI	CTCAG	7 cut(s)	75, 196, 682, 1022, 1655, 1914, 2298
BspDI	ATCGAT	3 cut(s)	407, 631, 2094
BspFNI	CGCG	5 cut(s)	11, 53, 80, 550, 1243
BspHI	TCATGA	2 cut(s)	697, 2064
BspLI	GGNNCC	3 cut(s)	714, 1152, 1342
BspMAI	CTGCAG	1 cut(s)	1905
BspMI	ACCTGC	1 cut(s)	1328
BspPI	GGATC	2 cut(s)	702, 1184
BspQI	GCTCTTC	2 cut(s)	14, 180
BspT107I	GGYRCC	2 cut(s)	712, 1340
BspTNI	GGTCTC	1 cut(s)	1920
BsrBI	CCGCTC	1 cut(s)	1752
BsrDI	GCAATG	1 cut(s)	1842
BsrFI	RCCGGY	1 cut(s)	1885
BsrGI	TGTACA	1 cut(s)	925
BsrI	ACTGG	5 cut(s)	793, 1107, 1422, 1672, 1977
BssAI	RCCGGY	1 cut(s)	1885
BssECI	CCNNGG	6 cut(s)	116, 766, 980, 1344, 1425, 1871
BssMI	GATC	7 cut(s)	69, 592, 632, 694, 745, 1138, 1189
BssSI	CACGAG	1 cut(s)	25
BssT1I	CCWWGG	4 cut(s)	980, 1344, 1425, 1871
Bst2BI	CACGAG	1 cut(s)	25
Bst2UI	CCWGG	3 cut(s)	118, 768, 1194
Bst6I	CTCTTC	3 cut(s)	14, 180, 2294
BstAUI	TGTACA	1 cut(s)	925
BstC8I	GCNNGC	3 cut(s)	760, 1689, 1887
BstDEI	CTNAG	9 cut(s)	62, 183, 690, 1009, 1495, 1642, 1922, 2252, 2306
BstDSI	CCRYGG	2 cut(s)	1344, 1425
BstEII	GGTNACC	1 cut(s)	292
BstENI	CCTNNNNNAGG	2 cut(s)	1158, 1197
BstF5I	GGATG	6 cut(s)	370, 762, 1061, 1804, 1881, 2139
BstFNI	CGCG	5 cut(s)	11, 53, 80, 550, 1243
BstH2I	RGCGCY	1 cut(s)	45
BstHHI	GCGC	2 cut(s)	11, 44
BstKTI	GATC	7 cut(s)	72, 595, 635, 697, 748, 1141, 1192
BstMAI	GTCTC	3 cut(s)	1158, 1645, 1920
BstMBI	GATC	7 cut(s)	69, 592, 632, 694, 745, 1138, 1189
BstMWI	GCNNNNNNNGC	8 cut(s)	50, 86, 685, 1229, 1334, 1371, 1940, 2042
BstNI	CCWGG	3 cut(s)	118, 768, 1194
BstNSI	RCATGY	1 cut(s)	762
BstPI	GGTNACC	1 cut(s)	292
BstSCI	CCNGG	6 cut(s)	116, 163, 399, 708, 766, 1192
BstSFI	CTRYAG	3 cut(s)	954, 1290, 1901
BstSLI	GKGCMC	2 cut(s)	324, 2173
BstUI	CGCG	5 cut(s)	11, 53, 80, 550, 1243
BstX2I	RGATCY	2 cut(s)	69, 745
BstYI	RGATCY	2 cut(s)	69, 745
Bsu15I	ATCGAT	3 cut(s)	407, 631, 2094
Bsu36I	CCTNAGG	1 cut(s)	690
BsuI	GTATCC	1 cut(s)	663
BsuRI	GGCC	9 cut(s)	77, 89, 168, 496, 708, 1074, 1412, 1885, 2250
BsuTUI	ATCGAT	3 cut(s)	407, 631, 2094
BtgI	CCRYGG	2 cut(s)	1344, 1425
BtsCI	GGATG	6 cut(s)	370, 762, 1061, 1804, 1881, 2139
BtsI	GCAGTG	1 cut(s)	324
BtsIMutI	CAGTG	6 cut(s)	324, 486, 786, 1287, 1442, 2299
BveI	ACCTGC	1 cut(s)	1328
Cac8I	GCNNGC	3 cut(s)	760, 1689, 1887
CaiI	CAGNNNCTG	1 cut(s)	1232
CciI	TCATGA	2 cut(s)	697, 2064
CfoI	GCGC	2 cut(s)	11, 44
Cfr10I	RCCGGY	1 cut(s)	1885
Cfr13I	GGNCC	5 cut(s)	75, 88, 166, 1022, 1411
ClaI	ATCGAT	3 cut(s)	407, 631, 2094
CseI	GACGC	1 cut(s)	42
Csp6I	GTAC	5 cut(s)	4, 82, 926, 1349, 1840
CspCI	CAANNNNNGTGG	2 cut(s)	2034, 2069
CviQI	GTAC	5 cut(s)	4, 82, 926, 1349, 1840
DdeI	CTNAG	9 cut(s)	62, 183, 690, 1009, 1495, 1642, 1922, 2252, 2306
DpnI	GATC	7 cut(s)	71, 594, 634, 696, 747, 1140, 1191
DpnII	GATC	7 cut(s)	69, 592, 632, 694, 745, 1138, 1189
DraIII	CACNNNGTG	1 cut(s)	1730
DrdI	GACNNNNNNGTC	1 cut(s)	1172
DseDI	GACNNNNNNGTC	1 cut(s)	1172
EaeI	YGGCCR	1 cut(s)	1072
Eam1104I	CTCTTC	3 cut(s)	14, 180, 2294
EarI	CTCTTC	3 cut(s)	14, 180, 2294
Ecl136II	GAGCTC	3 cut(s)	30, 100, 1081
Eco130I	CCWWGG	4 cut(s)	980, 1344, 1425, 1871
Eco147I	AGGCCT	1 cut(s)	496
Eco24I	GRGCYC	5 cut(s)	32, 63, 102, 1083, 1287
Eco31I	GGTCTC	1 cut(s)	1920
Eco32I	GATATC	1 cut(s)	370
Eco47I	GGWCC	1 cut(s)	1022
Eco53kI	GAGCTC	3 cut(s)	30, 100, 1081
Eco57I	CTGAAG	3 cut(s)	704, 939, 1383
Eco81I	CCTNAGG	1 cut(s)	690
Eco88I	CYCGRG	2 cut(s)	31, 2001
Eco91I	GGTNACC	1 cut(s)	292
EcoICRI	GAGCTC	3 cut(s)	30, 100, 1081
EcoNI	CCTNNNNNAGG	2 cut(s)	1158, 1197
EcoO109I	RGGNCCY	1 cut(s)	1411
EcoO65I	GGTNACC	1 cut(s)	292
EcoRI	GAATTC	1 cut(s)	992
EcoRII	CCWGG	3 cut(s)	116, 766, 1192
EcoRV	GATATC	1 cut(s)	370
EcoT14I	CCWWGG	4 cut(s)	980, 1344, 1425, 1871
EcoT22I	ATGCAT	2 cut(s)	760, 764
EcoT38I	GRGCYC	5 cut(s)	32, 63, 102, 1083, 1287
ErhI	CCWWGG	4 cut(s)	980, 1344, 1425, 1871
FauI	CCCGC	2 cut(s)	7, 1084
FbaI	TGATCA	1 cut(s)	1138
FokI	GGATG	6 cut(s)	377, 749, 1048, 1811, 1888, 2146
FriOI	GRGCYC	5 cut(s)	32, 63, 102, 1083, 1287
FspBI	CTAG	4 cut(s)	875, 936, 1199, 2007
GlaI	GCGC	2 cut(s)	10, 43
GsaI	CCCAGC	2 cut(s)	951, 1284
GsuI	CTGGAG	2 cut(s)	789, 1994
HaeII	RGCGCY	1 cut(s)	45
HaeIII	GGCC	9 cut(s)	77, 89, 168, 496, 708, 1074, 1412, 1885, 2250
HapII	CCGG	5 cut(s)	164, 401, 709, 1036, 1886
HgaI	GACGC	1 cut(s)	42
HhaI	GCGC	2 cut(s)	11, 44
Hin6I	GCGC	2 cut(s)	9, 42
HinP1I	GCGC	2 cut(s)	9, 42
HincII	GTYRAC	4 cut(s)	223, 336, 967, 2141
HindII	GTYRAC	4 cut(s)	223, 336, 967, 2141
HindIII	AAGCTT	1 cut(s)	449
HpaI	GTTAAC	1 cut(s)	223
HpaII	CCGG	5 cut(s)	164, 401, 709, 1036, 1886
HphI	GGTGA	4 cut(s)	790, 1199, 1996, 2321
Hpy166II	GTNNAC	8 cut(s)	223, 322, 336, 734, 926, 967, 2141, 2312
Hpy188I	TCNGA	9 cut(s)	240, 374, 1012, 1160, 1402, 1557, 1645, 2188, 2221
Hpy188III	TCNNGA	8 cut(s)	698, 740, 875, 1025, 1581, 1628, 2065, 2261
Hpy8I	GTNNAC	8 cut(s)	223, 322, 336, 734, 926, 967, 2141, 2312
Hpy99I	CGWCG	1 cut(s)	110
HpyAV	CCTTC	7 cut(s)	283, 698, 914, 1042, 1730, 1789, 2342
HpyCH4IV	ACGT	1 cut(s)	1859
HpyF10VI	GCNNNNNNNGC	8 cut(s)	50, 86, 685, 1229, 1334, 1371, 1940, 2042
HpyF3I	CTNAG	9 cut(s)	62, 183, 690, 1009, 1495, 1642, 1922, 2252, 2306
HpySE526I	ACGT	1 cut(s)	1859
HspAI	GCGC	2 cut(s)	9, 42
KroI	GCCGGC	1 cut(s)	1885
KroNI	GCCGGC	1 cut(s)	1887
Ksp22I	TGATCA	1 cut(s)	1138
KspAI	GTTAAC	1 cut(s)	223
Kzo9I	GATC	7 cut(s)	69, 592, 632, 694, 745, 1138, 1189
LguI	GCTCTTC	2 cut(s)	14, 180
LmnI	GCTCC	2 cut(s)	140, 676
LweI	GCATC	6 cut(s)	745, 771, 1414, 1730, 1789, 2032
MaeI	CTAG	4 cut(s)	875, 936, 1199, 2007
MaeII	ACGT	1 cut(s)	1859
MaeIII	GTNAC	5 cut(s)	292, 778, 784, 903, 1656
MalI	GATC	7 cut(s)	71, 594, 634, 696, 747, 1140, 1191
MbiI	CCGCTC	1 cut(s)	1752
MboI	GATC	7 cut(s)	69, 592, 632, 694, 745, 1138, 1189
MboII	GAAGA	9 cut(s)	31, 142, 167, 433, 587, 713, 755, 1823, 2311
MflI	RGATCY	2 cut(s)	69, 745
MhlI	GDGCHC	9 cut(s)	26, 32, 63, 102, 324, 1083, 1287, 1650, 2173
MlsI	TGGCCA	1 cut(s)	1074
MluCI	AATT	8 cut(s)	233, 811, 858, 888, 992, 1907, 2028, 2214
MluI	ACGCGT	2 cut(s)	51, 548
MluNI	TGGCCA	1 cut(s)	1074
MlyI	GAGTC	6 cut(s)	331, 810, 962, 1647, 1987, 2146
MmeI	TCCRAC	1 cut(s)	255
Mox20I	TGGCCA	1 cut(s)	1074
Mph1103I	ATGCAT	2 cut(s)	760, 764
MroNI	GCCGGC	1 cut(s)	1885
MscI	TGGCCA	1 cut(s)	1074
MslI	CAYNNNNRTG	3 cut(s)	586, 1134, 1745
Msp20I	TGGCCA	1 cut(s)	1074
MspA1I	CMGCKG	2 cut(s)	1232, 1691
MspI	CCGG	5 cut(s)	164, 401, 709, 1036, 1886
MspR9I	CCNGG	6 cut(s)	118, 165, 401, 710, 768, 1194
Mva1269I	GAATGC	1 cut(s)	1681
MvaI	CCWGG	3 cut(s)	118, 768, 1194
MvnI	CGCG	5 cut(s)	11, 53, 80, 550, 1243
MwoI	GCNNNNNNNGC	8 cut(s)	50, 86, 685, 1229, 1334, 1371, 1940, 2042
NaeI	GCCGGC	1 cut(s)	1887
NciI	CCSGG	3 cut(s)	165, 401, 710
NcoI	CCATGG	2 cut(s)	1344, 1425
NdeII	GATC	7 cut(s)	69, 592, 632, 694, 745, 1138, 1189
NgoMIV	GCCGGC	1 cut(s)	1885
NlaIV	GGNNCC	3 cut(s)	714, 1152, 1342
NmeAIII	GCCGAG	1 cut(s)	81
NmuCI	GTSAC	4 cut(s)	778, 784, 903, 1656
NsiI	ATGCAT	2 cut(s)	760, 764
NspI	RCATGY	1 cut(s)	762
PaeI	GCATGC	1 cut(s)	762
PaeR7I	CTCGAG	2 cut(s)	31, 2001
PagI	TCATGA	2 cut(s)	697, 2064
PaqCI	CACCTGC	1 cut(s)	1328
PceI	AGGCCT	1 cut(s)	496
PciSI	GCTCTTC	2 cut(s)	14, 180
PcsI	WCGNNNNNNNCGW	2 cut(s)	102, 201
PctI	GAATGC	1 cut(s)	1681
PdiI	GCCGGC	1 cut(s)	1887
PfeI	GAWTC	5 cut(s)	404, 932, 1307, 1702, 2072
PfoI	TCCNGGA	1 cut(s)	1192
PleI	GAGTC	6 cut(s)	331, 809, 962, 1646, 1986, 2145
PpsI	GAGTC	6 cut(s)	331, 809, 962, 1646, 1986, 2145
PsiI	TTATAA	1 cut(s)	1527
Psp124BI	GAGCTC	3 cut(s)	32, 102, 1083
Psp1406I	AACGTT	1 cut(s)	1859
Psp6I	CCWGG	3 cut(s)	116, 766, 1192
PspEI	GGTNACC	1 cut(s)	292
PspFI	CCCAGC	2 cut(s)	947, 1280
PspGI	CCWGG	3 cut(s)	116, 766, 1192
PspN4I	GGNNCC	3 cut(s)	714, 1152, 1342
PspPI	GGNCC	5 cut(s)	75, 88, 166, 1022, 1411
PspXI	VCTCGAGB	2 cut(s)	31, 2001
PstI	CTGCAG	1 cut(s)	1905
PstNI	CAGNNNCTG	1 cut(s)	1232
PsuI	RGATCY	2 cut(s)	69, 745
PvuII	CAGCTG	2 cut(s)	1232, 1691
RsaI	GTAC	5 cut(s)	5, 83, 927, 1350, 1841
RsaNI	GTAC	5 cut(s)	4, 82, 926, 1349, 1840
RseI	CAYNNNNRTG	3 cut(s)	586, 1134, 1745
SacI	GAGCTC	3 cut(s)	32, 102, 1083
SapI	GCTCTTC	2 cut(s)	14, 180
Sau3AI	GATC	7 cut(s)	69, 592, 632, 694, 745, 1138, 1189
Sau96I	GGNCC	5 cut(s)	75, 88, 166, 1022, 1411
SchI	GAGTC	6 cut(s)	331, 810, 962, 1647, 1987, 2146
ScrFI	CCNGG	6 cut(s)	118, 165, 401, 710, 768, 1194
SduI	GDGCHC	9 cut(s)	26, 32, 63, 102, 324, 1083, 1287, 1650, 2173
SfaNI	GCATC	6 cut(s)	745, 771, 1414, 1730, 1789, 2032
SfcI	CTRYAG	3 cut(s)	954, 1290, 1901
Sfr274I	CTCGAG	2 cut(s)	31, 2001
SinI	GGWCC	1 cut(s)	1022
SlaI	CTCGAG	2 cut(s)	31, 2001
SmiMI	CAYNNNNRTG	3 cut(s)	586, 1134, 1745
SmlI	CTYRAG	2 cut(s)	31, 2001
SmoI	CTYRAG	2 cut(s)	31, 2001
SphI	GCATGC	1 cut(s)	762
Sse9I	AATT	8 cut(s)	233, 811, 858, 888, 992, 1907, 2028, 2214
SseBI	AGGCCT	1 cut(s)	496
SsiI	CCGC	8 cut(s)	11, 14, 78, 161, 1091, 1241, 1752, 2158
SspI	AATATT	1 cut(s)	382
SspMI	CTAG	4 cut(s)	875, 936, 1199, 2007
SstI	GAGCTC	3 cut(s)	32, 102, 1083
StuI	AGGCCT	1 cut(s)	496
StyD4I	CCNGG	6 cut(s)	116, 163, 399, 708, 766, 1192
StyI	CCWWGG	4 cut(s)	980, 1344, 1425, 1871
TaiI	ACGT	1 cut(s)	1862
TaqI	TCGA	9 cut(s)	32, 96, 407, 631, 718, 839, 1386, 2002, 2094
TasI	AATT	8 cut(s)	233, 811, 858, 888, 992, 1907, 2028, 2214
TatI	WGTACW	2 cut(s)	925, 1839
TauI	GCSGC	3 cut(s)	14, 80, 163
TfiI	GAWTC	5 cut(s)	404, 932, 1307, 1702, 2072
TscAI	CASTG	6 cut(s)	324, 493, 793, 1294, 1442, 2299
TseFI	GTSAC	4 cut(s)	778, 784, 903, 1656
Tsp45I	GTSAC	4 cut(s)	778, 784, 903, 1656
TspGWI	ACGGA	1 cut(s)	2063
TspRI	CASTG	6 cut(s)	324, 493, 793, 1294, 1442, 2299
VneI	GTGCAC	1 cut(s)	320
VpaK11BI	GGWCC	1 cut(s)	1022
XagI	CCTNNNNNAGG	2 cut(s)	1158, 1197
XapI	RAATTY	4 cut(s)	233, 858, 992, 1907
XbaI	TCTAGA	1 cut(s)	874
XceI	RCATGY	1 cut(s)	762
XhoI	CTCGAG	2 cut(s)	31, 2001
XspI	CTAG	4 cut(s)	875, 936, 1199, 2007
Zsp2I	ATGCAT	2 cut(s)	760, 764

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.