Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr4

Orthogroup ID

OG0013292

Physical Location & Seq

Genomic Coordinates Forward (+)

12689221 .. 12693001

Gene Structure 3781 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom04g09950.10

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1449 bp

ATGACAGACCCATGGCACTTGCTGATCGATCTTTCCTCATTTTCCCCAAGGGATTTGGAATTCATTGTGAAGTTCTCTGGGGAACATGGTCCAGATACCTTCAGGCAACTACTTCAATCTATTTGTCCATCAATCTTTGGTCACGAGCTTGTTAAAGCGGGAATATTGCTGGCACTCTTTGGAGGTGTGCGGAAGAATTCAAATGATCAGAACAAGGTTCCTGTTAGAGGAGACATCCATGTGATAATTGTTGGTGATCCCGGACTAGGCAAGAGCCAGCTTCTCCAAGCAGCGGCAGCCATTTCTCCACGAGGCATTTATGTGTGTGGTAATGCCACAACCAAAGCTGGCCTCACTGTTGCTGTTGTAAAGGATCCTATGACAAGTGATTATGGTTTTGAAGCTGGTGCAATGGTAATTGCAGACAGTGGGTTGTGCTGTATTGATGAGTTTGACAAAATGTCTTCAGAGCATCAGGCTCTACTGGAAGCCATGGAACAACAGTGTGTCTCAGTCGCAAAGGCTGGACTGGTGGCAAGTTTGTCGGCACGAACTTCTGTCTTAGCAGCTGCAAATCCTGTTGGTGGTCATTATAACCGTGCAAAAACAGTGAATGAAAATTTGAAAATAAGTGCTGCTCTCTTATCGCGATTTGATTTGGTTTTCATATTACTTGACAAACCTAATGAACTTTTGGATAAGCGAGTCTCAGAACACATCATGTCACTTCATGCTGGATCTGGAGAAAATTCGCCAGCTGCAAAAAAGATTCGTAGAGCATCGCAGAGTGCAGAAGGCATAGATATCAGTGGAGAAGGTGGTTCTTTGGTTTCAAGATTGAGGCTTGACCCGAAGAAGGATGAGGATTTTGTTCCATTACCAGTACAACTCCTCCGCAAGTATATTGCTTATGCAAGAACTTTTGTGTTTCCTAGGATGTCAAAGCCTGCGGCAGAAATCCTGCAAAAGTTTTACTTAAAACTGAGAGACCATGCGACATCTGCTGATAGCACACCAATAACAGCAAGGCAGTTAGAGAGTCTGGTGAGGCTGACCGAAGCTCGAGCGAGGCTAGATTTGAGAGAAGAAATAACAGCCCAAGATGCAATGGATGTGGTTGAAATCATGAGAGAATCCTTGTATGACAAGTACATTGATGAGCATGGTGTTGTGGATTTTGGTCGGAGTGGAGGGATGAGTCAACAAAAAGAAGCAAAACGGTTCTTAAGTGCCCTCAATAGGCAATCAGAGTTGCAGCAGAAAGACTGTTTTACAATTTCTGAAATATACAGTTTGGCAGATAGGATTGGTCTAAGAGTTCCAGACATCGACACATTTGTTGACAACTTAAACAGTGTTGGTTATCTACTGAAGAAAGGAGCAAAGACATACCAGGTACTATCATCGTCTTATTCTCGGAGTCAATCGTCCTCAAGGTCAAGAGGCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000280 GO:0000724 GO:0000725 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006259 GO:0006281 GO:0006302 GO:0006310 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007049 GO:0007140 GO:0007143 GO:0007275 GO:0007276 GO:0007292 GO:0008150 GO:0008152 GO:0009555 GO:0009987 GO:0016043 GO:0019953 GO:0022402 GO:0022412 GO:0022414 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032504 GO:0032991 GO:0033554 GO:0034641 GO:0042555 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0044703 GO:0046483 GO:0048229 GO:0048232 GO:0048285 GO:0048609 GO:0048856 GO:0050896 GO:0051321 GO:0051704 GO:0051716 GO:0070013 GO:0071704 GO:0071840 GO:0090304 GO:0097362 GO:0140013 GO:1901360 GO:1903046

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MCM MCM_OB

Protein Analysis

483

Amino Acids

52.81

Weight (kDa)

7.64

Isoelectric Point (pI)

47.97

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013292)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G09660 AT3G09660 AT3G09660
fragaria_vesca	FvH4_6g15280 FvH4_6g15280
malus_domestica	MD04G1104500.v1.1
prunus_persica	Prupe.6G241500_v2.0.a1 Prupe.6G241500_v2.0.a1
pyrus_communis	pycom04g09950
rosa_chinensis	RchiOBHm_Chr3g0468711
rosa_laevigata	RLG00000024414
rosa_multiflora	Rmu_sc0011538.1_g000001
rosa_roxburghii	Rroxscaffold_6G00412640
rosa_rugosa	Rorug03G0096200
rosa_samantha	Rh3AG145200 Rh3BG167800 Rh3DG166500
rosa_wichuraiana	Rw3G013700

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	594
AccII	CGCG	1 cut(s)	649
AciI	CCGC	5 cut(s)	158, 190, 293, 895, 950
AclWI	GGATC	4 cut(s)	251, 368, 381, 745
AcsI	RAATTY	4 cut(s)	59, 196, 619, 748
AcuI	CTGAAG	3 cut(s)	85, 450, 1391
AfaI	GTAC	3 cut(s)	885, 1151, 1398
AfiI	CCNNNNNNNGG	6 cut(s)	227, 266, 292, 584, 856, 1239
AflII	CTTAAG	1 cut(s)	1225
AgsI	TTSAA	6 cut(s)	116, 201, 401, 625, 834, 1121
AhdI	GACNNNNNGTC	1 cut(s)	460
AjnI	CCWGG	1 cut(s)	1392
AluBI	AGCT	7 cut(s)	148, 280, 347, 404, 569, 758, 1061
AluI	AGCT	7 cut(s)	148, 280, 347, 404, 569, 758, 1061
Alw26I	GTCTC	4 cut(s)	225, 514, 712, 981
AlwI	GGATC	4 cut(s)	251, 368, 381, 745
Ama87I	CYCGRG	1 cut(s)	1062
AoxI	GGCC	1 cut(s)	349
ApeKI	GCWGC	7 cut(s)	290, 296, 566, 569, 635, 758, 1255
ApoI	RAATTY	4 cut(s)	59, 196, 619, 748
AspA2I	CCTAGG	1 cut(s)	932
AspS9I	GGNCC	1 cut(s)	89
AsuC2I	CCSGG	1 cut(s)	261
AsuHPI	GGTGA	2 cut(s)	266, 1057
AvaI	CYCGRG	1 cut(s)	1062
AvaII	GGWCC	1 cut(s)	89
AvrII	CCTAGG	1 cut(s)	932
BaeGI	GKGCMC	1 cut(s)	1234
BamHI	GGATCC	1 cut(s)	373
BauI	CACGAG	2 cut(s)	143, 309
BbsI	GAAGAC	1 cut(s)	456
BbvI	GCAGC	7 cut(s)	302, 308, 556, 578, 622, 745, 1267
BccI	CCATC	1 cut(s)	136
BcgI	CGANNNNNNTGC	2 cut(s)	525, 559
BciT130I	CCWGG	1 cut(s)	1394
BclI	TGATCA	1 cut(s)	205
BcnI	CCSGG	1 cut(s)	261
BcoDI	GTCTC	4 cut(s)	225, 514, 712, 981
BfaI	CTAG	3 cut(s)	266, 933, 1073
BfrI	CTTAAG	1 cut(s)	1225
BisI	GCNGC	9 cut(s)	291, 294, 297, 567, 570, 636, 759, 951, 1256
BlnI	CCTAGG	1 cut(s)	932
BlsI	GCNGC	9 cut(s)	292, 295, 298, 568, 571, 637, 760, 952, 1257
Bme1390I	CCNGG	2 cut(s)	261, 1394
Bme18I	GGWCC	1 cut(s)	89
BmeRI	GACNNNNNGTC	1 cut(s)	460
BmeT110I	CYCGRG	1 cut(s)	1062
BmgT120I	GGNCC	1 cut(s)	89
BmiI	GGNNCC	2 cut(s)	219, 375
BmrFI	CCNGG	2 cut(s)	261, 1394
BmsI	GCATC	3 cut(s)	481, 788, 1093
BpiI	GAAGAC	1 cut(s)	456
BpmI	CTGGAG	1 cut(s)	762
BpuEI	CTTGAG	1 cut(s)	1417
BpuMI	CCSGG	1 cut(s)	261
Bsa29I	ATCGAT	1 cut(s)	27
BsaI	GGTCTC	1 cut(s)	981
BsaJI	CCNNGG	4 cut(s)	11, 47, 492, 932
BsaXI	ACNNNNNCTCC	2 cut(s)	876, 906
Bsc4I	CCNNNNNNNGG	6 cut(s)	227, 266, 292, 584, 856, 1239
Bse1I	ACTGG	3 cut(s)	489, 534, 881
Bse3DI	GCAATG	2 cut(s)	417, 1113
BseBI	CCWGG	1 cut(s)	1394
BseCI	ATCGAT	1 cut(s)	27
BseDI	CCNNGG	4 cut(s)	11, 47, 492, 932
BseGI	GGATG	5 cut(s)	234, 865, 942, 1117, 1200
BseLI	CCNNNNNNNGG	6 cut(s)	227, 266, 292, 584, 856, 1239
BseMI	GCAATG	2 cut(s)	417, 1113
BseMII	CTCAG	3 cut(s)	525, 723, 974
BseNI	ACTGG	3 cut(s)	489, 534, 881
BseRI	GAGGAG	2 cut(s)	243, 881
BseSI	GKGCMC	1 cut(s)	1234
BseXI	GCAGC	7 cut(s)	302, 308, 556, 578, 622, 745, 1267
BsgI	GTGCAG	1 cut(s)	810
Bsh1236I	CGCG	1 cut(s)	649
BshFI	GGCC	1 cut(s)	351
BshVI	ATCGAT	1 cut(s)	27
BsiHKCI	CYCGRG	1 cut(s)	1062
BsiSI	CCGG	1 cut(s)	261
BslI	CCNNNNNNNGG	6 cut(s)	227, 266, 292, 584, 856, 1239
BsmAI	GTCTC	4 cut(s)	225, 514, 712, 981
BsnI	GGCC	1 cut(s)	351
Bso31I	GGTCTC	1 cut(s)	981
BsoBI	CYCGRG	1 cut(s)	1062
Bsp1286I	GDGCHC	1 cut(s)	1234
Bsp143I	GATC	6 cut(s)	24, 28, 205, 256, 373, 737
Bsp19I	CCATGG	2 cut(s)	11, 492
Bsp68I	TCGCGA	1 cut(s)	649
BspACI	CCGC	5 cut(s)	158, 190, 293, 895, 950
BspANI	GGCC	1 cut(s)	351
BspCNI	CTCAG	3 cut(s)	524, 722, 975
BspDI	ATCGAT	1 cut(s)	27
BspFNI	CGCG	1 cut(s)	649
BspHI	TCATGA	1 cut(s)	1125
BspLI	GGNNCC	2 cut(s)	219, 375
BspPI	GGATC	4 cut(s)	251, 368, 381, 745
BspTI	CTTAAG	1 cut(s)	1225
BspTNI	GGTCTC	1 cut(s)	981
BsrDI	GCAATG	2 cut(s)	417, 1113
BsrI	ACTGG	3 cut(s)	489, 534, 881
BssECI	CCNNGG	4 cut(s)	11, 47, 492, 932
BssMI	GATC	6 cut(s)	24, 28, 205, 256, 373, 737
BssSI	CACGAG	2 cut(s)	143, 309
BssT1I	CCWWGG	4 cut(s)	11, 47, 492, 932
Bst2BI	CACGAG	2 cut(s)	143, 309
Bst2UI	CCWGG	1 cut(s)	1394
Bst4CI	ACNGT	9 cut(s)	358, 428, 504, 599, 610, 1221, 1268, 1292, 1355
BstAFI	CTTAAG	1 cut(s)	1225
BstC8I	GCNNGC	5 cut(s)	171, 278, 349, 756, 948
BstDEI	CTNAG	5 cut(s)	511, 562, 709, 983, 1313
BstDSI	CCRYGG	2 cut(s)	11, 492
BstENI	CCTNNNNNAGG	1 cut(s)	225
BstF5I	GGATG	5 cut(s)	234, 865, 942, 1117, 1200
BstFNI	CGCG	1 cut(s)	649
BstKTI	GATC	6 cut(s)	27, 31, 208, 259, 376, 740
BstMAI	GTCTC	4 cut(s)	225, 514, 712, 981
BstMBI	GATC	6 cut(s)	24, 28, 205, 256, 373, 737
BstMWI	GCNNNNNNNGC	3 cut(s)	296, 1001, 1103
BstNI	CCWGG	1 cut(s)	1394
BstSCI	CCNGG	2 cut(s)	259, 1392
BstSLI	GKGCMC	1 cut(s)	1234
BstUI	CGCG	1 cut(s)	649
BstV1I	GCAGC	7 cut(s)	302, 308, 556, 578, 622, 745, 1267
BstV2I	GAAGAC	1 cut(s)	456
BstX2I	RGATCY	2 cut(s)	373, 737
BstYI	RGATCY	2 cut(s)	373, 737
Bsu15I	ATCGAT	1 cut(s)	27
BsuRI	GGCC	1 cut(s)	351
BsuTUI	ATCGAT	1 cut(s)	27
BtgI	CCRYGG	2 cut(s)	11, 492
BtgZI	GCGATG	1 cut(s)	765
BtsCI	GGATG	5 cut(s)	234, 865, 942, 1117, 1200
BtsIMutI	CAGTG	6 cut(s)	354, 433, 509, 615, 814, 1360
BtuMI	TCGCGA	1 cut(s)	649
Cac8I	GCNNGC	5 cut(s)	171, 278, 349, 756, 948
CciI	TCATGA	1 cut(s)	1125
Cfr13I	GGNCC	1 cut(s)	89
ClaI	ATCGAT	1 cut(s)	27
CsiI	ACCWGGT	1 cut(s)	1392
Csp6I	GTAC	3 cut(s)	884, 1150, 1397
CspCI	CAANNNNNGTGG	2 cut(s)	1095, 1130
CviAII	CATG	9 cut(s)	12, 86, 239, 493, 721, 731, 992, 1126, 1163
CviQI	GTAC	3 cut(s)	884, 1150, 1397
DdeI	CTNAG	5 cut(s)	511, 562, 709, 983, 1313
DpnI	GATC	6 cut(s)	26, 30, 207, 258, 375, 739
DpnII	GATC	6 cut(s)	24, 28, 205, 256, 373, 737
DriI	GACNNNNNGTC	1 cut(s)	460
Eam1105I	GACNNNNNGTC	1 cut(s)	460
Eco130I	CCWWGG	4 cut(s)	11, 47, 492, 932
Eco31I	GGTCTC	1 cut(s)	981
Eco32I	GATATC	1 cut(s)	805
Eco47I	GGWCC	1 cut(s)	89
Eco57I	CTGAAG	3 cut(s)	85, 450, 1391
Eco88I	CYCGRG	1 cut(s)	1062
EcoNI	CCTNNNNNAGG	1 cut(s)	225
EcoRI	GAATTC	2 cut(s)	59, 196
EcoRII	CCWGG	1 cut(s)	1392
EcoRV	GATATC	1 cut(s)	805
EcoT14I	CCWWGG	4 cut(s)	11, 47, 492, 932
ErhI	CCWWGG	4 cut(s)	11, 47, 492, 932
FaeI	CATG	9 cut(s)	15, 89, 242, 496, 724, 734, 995, 1129, 1166
FalI	AAGNNNNNCTT	2 cut(s)	959, 991
FatI	CATG	9 cut(s)	11, 85, 238, 492, 720, 730, 991, 1125, 1162
FauI	CCCGC	1 cut(s)	151
FbaI	TGATCA	1 cut(s)	205
Fnu4HI	GCNGC	9 cut(s)	291, 294, 297, 567, 570, 636, 759, 951, 1256
FokI	GGATG	5 cut(s)	221, 872, 949, 1124, 1207
Fsp4HI	GCNGC	9 cut(s)	291, 294, 297, 567, 570, 636, 759, 951, 1256
FspBI	CTAG	3 cut(s)	266, 933, 1073
GluI	GCNGC	9 cut(s)	291, 294, 297, 567, 570, 636, 759, 951, 1256
GsuI	CTGGAG	1 cut(s)	762
HaeIII	GGCC	1 cut(s)	351
HapII	CCGG	1 cut(s)	261
Hin1II	CATG	9 cut(s)	15, 89, 242, 496, 724, 734, 995, 1129, 1166
HincII	GTYRAC	2 cut(s)	1202, 1342
HindII	GTYRAC	2 cut(s)	1202, 1342
HinfI	GANTC	6 cut(s)	705, 769, 1039, 1133, 1198, 1420
HpaII	CCGG	1 cut(s)	261
HphI	GGTGA	2 cut(s)	266, 1057
Hpy166II	GTNNAC	2 cut(s)	1202, 1342
Hpy188I	TCNGA	7 cut(s)	210, 469, 712, 1185, 1249, 1282, 1419
Hpy188III	TCNNGA	8 cut(s)	92, 143, 648, 741, 834, 1126, 1322, 1440
Hpy8I	GTNNAC	2 cut(s)	1202, 1342
HpyAV	CCTTC	4 cut(s)	109, 788, 809, 850
HpyCH4III	ACNGT	9 cut(s)	358, 428, 504, 599, 610, 1221, 1268, 1292, 1355
HpyF10VI	GCNNNNNNNGC	3 cut(s)	296, 1001, 1103
HpyF3I	CTNAG	5 cut(s)	511, 562, 709, 983, 1313
Hsp92II	CATG	9 cut(s)	15, 89, 242, 496, 724, 734, 995, 1129, 1166
Ksp22I	TGATCA	1 cut(s)	205
Kzo9I	GATC	6 cut(s)	24, 28, 205, 256, 373, 737
LmnI	GCTCC	1 cut(s)	1379
Lsp1109I	GCAGC	7 cut(s)	302, 308, 556, 578, 622, 745, 1267
LweI	GCATC	3 cut(s)	481, 788, 1093
MabI	ACCWGGT	1 cut(s)	1392
MaeI	CTAG	3 cut(s)	266, 933, 1073
MaeIII	GTNAC	2 cut(s)	140, 723
MalI	GATC	6 cut(s)	26, 30, 207, 258, 375, 739
MboI	GATC	6 cut(s)	24, 28, 205, 256, 373, 737
MboII	GAAGA	5 cut(s)	205, 456, 865, 1097, 1384
MflI	RGATCY	2 cut(s)	373, 737
MhlI	GDGCHC	1 cut(s)	1234
MluCI	AATT	7 cut(s)	59, 196, 246, 417, 619, 748, 1275
MlyI	GAGTC	4 cut(s)	714, 1048, 1207, 1429
MmeI	TCCRAC	1 cut(s)	1163
MseI	TTAA	4 cut(s)	153, 977, 1226, 1349
MslI	CAYNNNNRTG	2 cut(s)	239, 320
MspA1I	CMGCKG	3 cut(s)	293, 569, 758
MspCI	CTTAAG	1 cut(s)	1225
MspI	CCGG	1 cut(s)	261
MspR9I	CCNGG	2 cut(s)	261, 1394
MvaI	CCWGG	1 cut(s)	1394
MvnI	CGCG	1 cut(s)	649
MwoI	GCNNNNNNNGC	3 cut(s)	296, 1001, 1103
NciI	CCSGG	1 cut(s)	261
NcoI	CCATGG	2 cut(s)	11, 492
NdeII	GATC	6 cut(s)	24, 28, 205, 256, 373, 737
NlaIII	CATG	9 cut(s)	15, 89, 242, 496, 724, 734, 995, 1129, 1166
NlaIV	GGNNCC	2 cut(s)	219, 375
NmuCI	GTSAC	2 cut(s)	140, 723
NruI	TCGCGA	1 cut(s)	649
PaeR7I	CTCGAG	1 cut(s)	1062
PagI	TCATGA	1 cut(s)	1125
PfeI	GAWTC	2 cut(s)	769, 1133
PfoI	TCCNGGA	1 cut(s)	259
PkrI	GCNGC	9 cut(s)	292, 295, 298, 568, 571, 637, 760, 952, 1257
PleI	GAGTC	4 cut(s)	713, 1047, 1206, 1428
PpsI	GAGTC	4 cut(s)	713, 1047, 1206, 1428
PsiI	TTATAA	1 cut(s)	594
Psp6I	CCWGG	1 cut(s)	1392
PspGI	CCWGG	1 cut(s)	1392
PspN4I	GGNNCC	2 cut(s)	219, 375
PspPI	GGNCC	1 cut(s)	89
PspXI	VCTCGAGB	1 cut(s)	1062
PsuI	RGATCY	2 cut(s)	373, 737
PvuII	CAGCTG	2 cut(s)	569, 758
RruI	TCGCGA	1 cut(s)	649
RsaI	GTAC	3 cut(s)	885, 1151, 1398
RsaNI	GTAC	3 cut(s)	884, 1150, 1397
RseI	CAYNNNNRTG	2 cut(s)	239, 320
SaqAI	TTAA	4 cut(s)	153, 977, 1226, 1349
SatI	GCNGC	9 cut(s)	291, 294, 297, 567, 570, 636, 759, 951, 1256
Sau3AI	GATC	6 cut(s)	24, 28, 205, 256, 373, 737
Sau96I	GGNCC	1 cut(s)	89
SchI	GAGTC	4 cut(s)	714, 1048, 1207, 1429
ScrFI	CCNGG	2 cut(s)	261, 1394
SduI	GDGCHC	1 cut(s)	1234
SexAI	ACCWGGT	1 cut(s)	1392
SfaNI	GCATC	3 cut(s)	481, 788, 1093
Sfr274I	CTCGAG	1 cut(s)	1062
SinI	GGWCC	1 cut(s)	89
SlaI	CTCGAG	1 cut(s)	1062
SmiMI	CAYNNNNRTG	2 cut(s)	239, 320
SmlI	CTYRAG	3 cut(s)	1062, 1225, 1432
SmoI	CTYRAG	3 cut(s)	1062, 1225, 1432
Sse9I	AATT	7 cut(s)	59, 196, 246, 417, 619, 748, 1275
SsiI	CCGC	5 cut(s)	158, 190, 293, 895, 950
SspI	AATATT	1 cut(s)	165
SspMI	CTAG	3 cut(s)	266, 933, 1073
StyD4I	CCNGG	2 cut(s)	259, 1392
StyI	CCWWGG	4 cut(s)	11, 47, 492, 932
TaaI	ACNGT	9 cut(s)	358, 428, 504, 599, 610, 1221, 1268, 1292, 1355
TaqI	TCGA	3 cut(s)	27, 1063, 1329
TaqII	GACCGA	1 cut(s)	1070
TasI	AATT	7 cut(s)	59, 196, 246, 417, 619, 748, 1275
TatI	WGTACW	2 cut(s)	883, 1149
TauI	GCSGC	2 cut(s)	296, 953
TfiI	GAWTC	2 cut(s)	769, 1133
Tru1I	TTAA	4 cut(s)	153, 977, 1226, 1349
Tru9I	TTAA	4 cut(s)	153, 977, 1226, 1349
TscAI	CASTG	6 cut(s)	361, 433, 509, 615, 814, 1360
TseFI	GTSAC	2 cut(s)	140, 723
TseI	GCWGC	7 cut(s)	290, 296, 566, 569, 635, 758, 1255
Tsp45I	GTSAC	2 cut(s)	140, 723
TspDTI	ATGAA	5 cut(s)	52, 630, 655, 702, 719
TspRI	CASTG	6 cut(s)	361, 433, 509, 615, 814, 1360
Vha464I	CTTAAG	1 cut(s)	1225
VpaK11BI	GGWCC	1 cut(s)	89
XagI	CCTNNNNNAGG	1 cut(s)	225
XapI	RAATTY	4 cut(s)	59, 196, 619, 748
XhoI	CTCGAG	1 cut(s)	1062
XmaJI	CCTAGG	1 cut(s)	932
XspI	CTAG	3 cut(s)	266, 933, 1073

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.