FvH4_6g18180

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0005763
Physical Location & Seq
Reverse (-)
11993739 .. 11998878
5140 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g18180.t1

Sequence Viewer

Length: 2715 bp
ATGGGTTCATTTGCGGGTACTTCTGAAATTGTTGAAGCGACAGACGAGCCCAGCTCAGGTCAATATTCTCGTGCGACATATCGACCGAATTCGGGGTTTGGTGAGAATGATAAGAAGCTTCCTGCACTTAAACTAGGGTCATCCAGAGATTCTATAGAAGATGATATTAATAAGCTTTTTGCGGCAATCCATCTCAAAACTTCATCCAAGGGTTCAGATCATTCAAAGCAAATAGCCACTAGCCCTTTGAACAAGAGTGCCTTAAAAAAGCCAATTACAGTGGGTGTGTCTCACTCACCAAGGATTGGGACTTCTGAGCCAGCAACTCTAAAGCAGGCATTAAGGGAGCTGTCTATTACCAAGGCATCAGAAATGGCTGCTATGAAACGGTTAACAAAGTTAACAAGTCCTTCAAGAGCCTCTGAAGCTGGGAGGATCAAGACATTGTACAATGCGGTTGTTGTTCAAGCCACTCAATGCGGTCCTTCCTCACATGAAGACAGGGGAAATGTAGTTGAAATCTCTCTGGTCCCGGAAGAAAGCACATCATATTCACGTGAGAAGATGCCTGAGCATCCTCCAATGCCCAAGACGAAGTCATCAAGCCAGACTGCACATTCTTCTCCTCGATTTGTAAATGGGACAACAAAAAATAGTGCTGGGTGCACAGCAGTACAAAATGAAAGTGGTTCCACGGTGAGGACGGTTGGGGTACAAACAATAAAGTCGGAGCTGGGTCTAGAAAATGAAAATGAATCTGGTAGCGAGCTTATTTCTGAAGTTGCTGAAAATGCACCTGTAAAAGCTGTTTTGAAGACAACATTAAAGGCAGAGCTGGGGAAGAAAGTGAAACGAAAGTCCACAAAGGAAAACAATTCAGTTTCTTATAGAAATAGTCCTACCAAAACCAAGTTAGTAAATAAGGTTTCAGCAGCAAAAGCGGGCCGAAAAGTCAAGGTGCATGGAGTGCCTTCTTCATCTAGCTTTGTCAATGGAAATGTGGCAAGCAAGTTTTCCAGAAACACACTCCGCAGTATCAAACCATCCAGCAGGAATAAAAGTATTGTTAAGAAGAAGCAAAACCAAGATTCAGTTTCTCCTGCCTGCAAGTCTAATAAGGGTAATGGAGCTGATGCCGATATCGATCCATGTACAAGTCAGCTGGTCTGTGAGAGATGCCATATTGCCTTAAAAGGTGCAGGAAAACAATACAATCAAGCTTTGGAAACCCCCACTCTTGCTGCTGAAATAAGCTCAAGCAATGTACAGTCCACTCCAAGTAAACCAGGCTTCACCGTAGACAGCTGTAAGACTGGTAGTAGAGGTGGAGGTGATGTCGTGAAAGCAAATAAGAACCCAAAATCAAAAGGAACAGGGGAGTTCTCCCAAAGCTCAAAAAGTAGCCAAGGTGAGTATAGTAGTAGTACAAGTTTGAGTGATGAGAGTAATTTAAGTAGGACTAGTTGTAGCAATAAACCTCACATGTCGAAGGATGTGAGGTGGGAAGCCATCCGTCATGTTCGGATGCAGCATGGAGCCCTGGGCTTGAAACACTTCAATCTTTTGAAGAAGCTTGGTTGTGGAGACATTGGAACTGTATATCTTGCTGAGCTAATCAGTACAAACTGCCTTTTTGCCATAAAGGTAATGGACAATGAGTTCTTGTCAAGAAGGAAGAAGATGCCAAGGGCTCACACAGAAAGTGAAATACTGAGGATGCTTGATCATCCTTTTCTCCCTACATTATATTCCCAGTTTACATCAGATAATCTTTCATGTTTAGTAATGGAGTATTGTCCTGGGGGAGACCTTCATGTCCTCCGGCAGAAGCAGGTCGGAAGGTGTTTTTCTGAAGCAGCTACAAGGTTTTATGTGGCTGAAGTCCTCCTCGCTTTGGAGTACTTGCACATGCTTGGTATAGTTTATCGGGATCTGAAGCCAGAAAACATTCTTGTCCGGGAAGATGGTCACATTATGCTCACAGATTTTGACCTGTCTCTTAGATGCTCTGTCAATCCAACTCTTTTGAAATCACCTTCATGTGATGTGGATCCTGCAAGAATGTCGGGTCCATGTACAGCATCTAGCTGCGCCGAGCCCTTCTGCATTGAGCCCTCCTCTTGTCAAGTCTCATGCTTTAGCCCTAGGTTTTTACCTGCTGCCGCAAAAGCAAGGAAGGCAAAAAATGACGCTGCATCTCAAGTCAGATCATTGCCACAGCTTGTGGCAGAGCCTAGCGACGCAAGGTCCAATTCCTTTGTTGGGACCCACGAGTACTTGGCTCCTGAGATCATCAAAGGAGACGGTCATGGTGCTGCAGTTGATTGGTGGACGTTTGGTGTCTTTCTTTACGAGCTTCTATATGGCAGAACACCATTTAAGGGTGCTACCAATGAGGAAACATTAGGCAATGTGGTCTTGCAGAGCCTAAGATTCCCTGACACCCCAATTGTTAGCTTCCATGCTAGGGATCTCATCAGAGCTCTGTTGGTAAAGGAGCCAGAGAATCGGTTGGGCACGGAGAAAGGAGCTGGTGAGATTAAGCAACATCCGTTCTTTGAGGGTTTGAACTGGGCACTAATCCGCTGTGCTATTCCGCCGGAACTGCCTGATTTTTGTGATTTTGACGTTCCAGACATGCCTGTTCAGGACGATGACACCAAGTATTTGGAGTTTAAAGCAACGGGAGGCCACCTAGAGTTTGAGTTATTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

905

Amino Acids

98.28

Weight (kDa)

8.96

Isoelectric Point (pI)

48.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 519 - 669 2.9e-36 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 520 - 669 1.8e-19 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 749 - 853 1.3e-19 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 757 - 838 8.2e-07 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1814
Acc36I ACCTGC 2 cut(s) 1822, 2164
AccB7I CCANNNNNTGG 1 cut(s) 305
AccI GTMKAC 1 cut(s) 1299
AciI CCGC 9 cut(s) 14, 182, 455, 480, 941, 1030, 2163, 2584, 2597
AclWI GGATC 6 cut(s) 443, 1139, 1938, 2045, 2058, 2478
AcsI RAATTY 1 cut(s) 88
AcuI CTGAAG 5 cut(s) 444, 798, 1872, 1899, 1955
AcvI CACGTG 1 cut(s) 557
AfiI CCNNNNNNNGG 8 cut(s) 56, 92, 305, 699, 1894, 2262, 2381, 2467
AflIII ACRYGT 1 cut(s) 1482
AhdI GACNNNNNGTC 1 cut(s) 2245
AhlI ACTAGT 1 cut(s) 1460
AjnI CCWGG 3 cut(s) 1285, 1539, 1798
AloI GAACNNNNNNTCC 2 cut(s) 1643, 1675
Alw21I GWGCWC 2 cut(s) 668, 2485
Alw26I GTCTC 6 cut(s) 294, 1578, 1800, 2001, 2134, 2295
Alw44I GTGCAC 1 cut(s) 664
AlwI GGATC 6 cut(s) 443, 1139, 1938, 2045, 2058, 2478
AoxI GGCC 2 cut(s) 943, 2689
ApaLI GTGCAC 1 cut(s) 664
ApeKI GCWGC 9 cut(s) 377, 932, 1241, 1528, 1856, 2088, 2159, 2192, 2315
ApoI RAATTY 1 cut(s) 88
AseI ATTAAT 1 cut(s) 168
Asp700I GAANNNNTTC 2 cut(s) 1553, 1947
AspA2I CCTAGG 1 cut(s) 2144
AspLEI GCGC 1 cut(s) 2093
AspS9I GGNCC 6 cut(s) 482, 529, 943, 2069, 2247, 2265
AsuC2I CCSGG 2 cut(s) 533, 1958
AsuHPI GGTGA 8 cut(s) 113, 288, 709, 1285, 1343, 1421, 2025, 2546
AvaII GGWCC 5 cut(s) 482, 529, 2069, 2247, 2265
AvrII CCTAGG 1 cut(s) 2144
BaeGI GKGCMC 3 cut(s) 668, 2519, 2578
BamHI GGATCC 1 cut(s) 2050
BanII GRGCYC 6 cut(s) 51, 1540, 1693, 2100, 2115, 2485
BauI CACGAG 2 cut(s) 69, 2270
BbrPI CACGTG 1 cut(s) 557
BbsI GAAGAC 2 cut(s) 504, 821
Bbv12I GWGCWC 2 cut(s) 668, 2485
BbvI GCAGC 9 cut(s) 364, 944, 1228, 1540, 1868, 2075, 2146, 2179, 2302
BccI CCATC 4 cut(s) 198, 1051, 1517, 1958
BcgI CGANNNNNNTGC 2 cut(s) 2046, 2080
BciT130I CCWGG 3 cut(s) 1287, 1541, 1800
BclI TGATCA 1 cut(s) 1723
BcnI CCSGG 2 cut(s) 533, 1958
BcoDI GTCTC 6 cut(s) 294, 1578, 1800, 2001, 2134, 2295
BcuI ACTAGT 1 cut(s) 1460
BfmI CTRYAG 2 cut(s) 153, 2316
BfuAI ACCTGC 2 cut(s) 1822, 2164
BlnI CCTAGG 1 cut(s) 2144
BlpI GCTNAGC 1 cut(s) 1608
BmcAI AGTACT 2 cut(s) 1901, 2276
Bme1390I CCNGG 5 cut(s) 533, 1287, 1541, 1800, 1958
Bme18I GGWCC 5 cut(s) 482, 529, 2069, 2247, 2265
BmeRI GACNNNNNGTC 1 cut(s) 2245
BmgT120I GGNCC 6 cut(s) 482, 529, 943, 2069, 2247, 2265
BmiI GGNNCC 9 cut(s) 531, 691, 1537, 2052, 2070, 2266, 2267, 2283, 2499
BmrFI CCNGG 5 cut(s) 533, 1287, 1541, 1800, 1958
BmrI ACTGGG 2 cut(s) 1747, 2581
BmuI ACTGGG 2 cut(s) 1747, 2581
BpiI GAAGAC 2 cut(s) 504, 821
BplI GAGNNNNNCTC 4 cut(s) 38, 70, 2102, 2134
Bpu10I CCTNAGC 2 cut(s) 55, 570
Bpu1102I GCTNAGC 1 cut(s) 1608
BpuEI CTTGAG 2 cut(s) 1240, 2184
BpuMI CCSGG 2 cut(s) 533, 1958
Bsa29I ATCGAT 1 cut(s) 1143
BsaAI YACGTR 1 cut(s) 557
BsaBI GATNNNNATC 2 cut(s) 1143, 2049
BsaI GGTCTC 1 cut(s) 1800
BsaXI ACNNNNNCTCC 2 cut(s) 1320, 1350
Bsc4I CCNNNNNNNGG 8 cut(s) 56, 92, 305, 699, 1894, 2262, 2381, 2467
Bse1I ACTGG 3 cut(s) 1318, 1753, 2576
Bse3DI GCAATG 3 cut(s) 1267, 2210, 2416
Bse8I GATNNNNATC 2 cut(s) 1143, 2049
BseBI CCWGG 3 cut(s) 1287, 1541, 1800
BseCI ATCGAT 1 cut(s) 1143
BseJI GATNNNNATC 2 cut(s) 1143, 2049
BseLI CCNNNNNNNGG 8 cut(s) 56, 92, 305, 699, 1894, 2262, 2381, 2467
BseMI GCAATG 3 cut(s) 1267, 2210, 2416
BseMII CTCAG 6 cut(s) 69, 306, 561, 1599, 1703, 2277
BseNI ACTGG 3 cut(s) 1318, 1753, 2576
BseRI GAGGAG 3 cut(s) 615, 1877, 2107
BseSI GKGCMC 3 cut(s) 668, 2519, 2578
BseXI GCAGC 9 cut(s) 364, 944, 1228, 1540, 1868, 2075, 2146, 2179, 2302
BseYI CCCAGC 5 cut(s) 50, 428, 659, 733, 835
BsgI GTGCAG 3 cut(s) 108, 597, 1218
Bsh1285I CGRYCG 1 cut(s) 86
BshFI GGCC 2 cut(s) 945, 2691
BshVI ATCGAT 1 cut(s) 1143
BsiEI CGRYCG 1 cut(s) 86
BsiHKAI GWGCWC 2 cut(s) 668, 2485
BsiSI CCGG 4 cut(s) 533, 1822, 1957, 2600
BslFI GGGAC 4 cut(s) 322, 515, 655, 2278
BslI CCNNNNNNNGG 8 cut(s) 56, 92, 305, 699, 1894, 2262, 2381, 2467
BsmAI GTCTC 6 cut(s) 294, 1578, 1800, 2001, 2134, 2295
BsmBI CGTCTC 1 cut(s) 2295
BsmFI GGGAC 4 cut(s) 322, 515, 655, 2278
BsnI GGCC 2 cut(s) 945, 2691
Bso31I GGTCTC 1 cut(s) 1800
Bsp1286I GDGCHC 9 cut(s) 51, 668, 1540, 1693, 2100, 2115, 2485, 2519, 2578
Bsp1407I TGTACA 4 cut(s) 447, 1151, 1264, 2075
Bsp143I GATC 9 cut(s) 217, 435, 1144, 1723, 1930, 2050, 2207, 2289, 2470
Bsp1720I GCTNAGC 1 cut(s) 1608
BspACI CCGC 9 cut(s) 14, 182, 455, 480, 941, 1030, 2163, 2584, 2597
BspANI GGCC 2 cut(s) 945, 2691
BspCNI CTCAG 6 cut(s) 68, 307, 562, 1600, 1704, 2278
BspDI ATCGAT 1 cut(s) 1143
BspLI GGNNCC 9 cut(s) 531, 691, 1537, 2052, 2070, 2266, 2267, 2283, 2499
BspMAI CTGCAG 1 cut(s) 2320
BspMI ACCTGC 2 cut(s) 1822, 2164
BspPI GGATC 6 cut(s) 443, 1139, 1938, 2045, 2058, 2478
BspTNI GGTCTC 1 cut(s) 1800
BsrDI GCAATG 3 cut(s) 1267, 2210, 2416
BsrGI TGTACA 4 cut(s) 447, 1151, 1264, 2075
BsrI ACTGG 3 cut(s) 1318, 1753, 2576
BssMI GATC 9 cut(s) 217, 435, 1144, 1723, 1930, 2050, 2207, 2289, 2470
BssSI CACGAG 2 cut(s) 69, 2270
BssT1I CCWWGG 6 cut(s) 207, 299, 360, 1405, 1685, 2144
Bst2BI CACGAG 2 cut(s) 69, 2270
Bst2UI CCWGG 3 cut(s) 1287, 1541, 1800
Bst4CI ACNGT 8 cut(s) 280, 390, 697, 706, 1269, 1297, 1597, 2306
BstAPI GCANNNNNTGC 1 cut(s) 967
BstAUI TGTACA 4 cut(s) 447, 1151, 1264, 2075
BstBAI YACGTR 1 cut(s) 557
BstC8I GCNNGC 6 cut(s) 321, 336, 767, 943, 1006, 1105
BstDEI CTNAG 8 cut(s) 55, 315, 570, 1608, 1712, 2000, 2286, 2429
BstDSI CCRYGG 1 cut(s) 693
BstHHI GCGC 1 cut(s) 2093
BstKTI GATC 9 cut(s) 220, 438, 1147, 1726, 1933, 2053, 2210, 2292, 2473
BstMAI GTCTC 6 cut(s) 294, 1578, 1800, 2001, 2134, 2295
BstMBI GATC 9 cut(s) 217, 435, 1144, 1723, 1930, 2050, 2207, 2289, 2470
BstMCI CGRYCG 1 cut(s) 86
BstMWI GCNNNNNNNGC 7 cut(s) 425, 791, 938, 967, 2168, 2177, 2605
BstNI CCWGG 3 cut(s) 1287, 1541, 1800
BstNSI RCATGY 3 cut(s) 1486, 1912, 2641
BstSCI CCNGG 5 cut(s) 531, 1285, 1539, 1798, 1956
BstSFI CTRYAG 2 cut(s) 153, 2316
BstSLI GKGCMC 3 cut(s) 668, 2519, 2578
BstV1I GCAGC 9 cut(s) 364, 944, 1228, 1540, 1868, 2075, 2146, 2179, 2302
BstV2I GAAGAC 2 cut(s) 504, 821
BstX2I RGATCY 3 cut(s) 1930, 2050, 2470
BstXI CCANNNNNNTGG 1 cut(s) 2668
BstYI RGATCY 3 cut(s) 1930, 2050, 2470
Bsu15I ATCGAT 1 cut(s) 1143
BsuRI GGCC 2 cut(s) 945, 2691
BsuTUI ATCGAT 1 cut(s) 1143
BtgI CCRYGG 1 cut(s) 693
BtsIMutI CAGTG 1 cut(s) 285
BveI ACCTGC 2 cut(s) 1822, 2164
Cac8I GCNNGC 6 cut(s) 321, 336, 767, 943, 1006, 1105
CfoI GCGC 1 cut(s) 2093
Cfr13I GGNCC 6 cut(s) 482, 529, 943, 2069, 2247, 2265
ClaI ATCGAT 1 cut(s) 1143
CseI GACGC 2 cut(s) 2198, 2249
CspCI CAANNNNNGTGG 2 cut(s) 261, 296
DdeI CTNAG 8 cut(s) 55, 315, 570, 1608, 1712, 2000, 2286, 2429
DpnI GATC 9 cut(s) 219, 437, 1146, 1725, 1932, 2052, 2209, 2291, 2472
DpnII GATC 9 cut(s) 217, 435, 1144, 1723, 1930, 2050, 2207, 2289, 2470
DraI TTTAAA 1 cut(s) 2677
DrdI GACNNNNNNGTC 1 cut(s) 1814
DriI GACNNNNNGTC 1 cut(s) 2245
DseDI GACNNNNNNGTC 1 cut(s) 1814
Eam1105I GACNNNNNGTC 1 cut(s) 2245
EciI GGCGGA 1 cut(s) 2586
Ecl136II GAGCTC 1 cut(s) 2483
Eco130I CCWWGG 6 cut(s) 207, 299, 360, 1405, 1685, 2144
Eco24I GRGCYC 6 cut(s) 51, 1540, 1693, 2100, 2115, 2485
Eco31I GGTCTC 1 cut(s) 1800
Eco32I GATATC 1 cut(s) 1141
Eco47I GGWCC 5 cut(s) 482, 529, 2069, 2247, 2265
Eco53kI GAGCTC 1 cut(s) 2483
Eco57I CTGAAG 5 cut(s) 444, 798, 1872, 1899, 1955
Eco72I CACGTG 1 cut(s) 557
EcoICRI GAGCTC 1 cut(s) 2483
EcoO109I RGGNCCY 1 cut(s) 2265
EcoRI GAATTC 1 cut(s) 88
EcoRII CCWGG 3 cut(s) 1285, 1539, 1798
EcoRV GATATC 1 cut(s) 1141
EcoT14I CCWWGG 6 cut(s) 207, 299, 360, 1405, 1685, 2144
EcoT38I GRGCYC 6 cut(s) 51, 1540, 1693, 2100, 2115, 2485
ErhI CCWWGG 6 cut(s) 207, 299, 360, 1405, 1685, 2144
Esp3I CGTCTC 1 cut(s) 2295
FalI AAGNNNNNCTT 2 cut(s) 245, 277
FaqI GGGAC 4 cut(s) 322, 515, 655, 2278
FauI CCCGC 2 cut(s) 7, 934
FbaI TGATCA 1 cut(s) 1723
FblI GTMKAC 1 cut(s) 1299
FriOI GRGCYC 6 cut(s) 51, 1540, 1693, 2100, 2115, 2485
GlaI GCGC 1 cut(s) 2092
GsaI CCCAGC 5 cut(s) 54, 432, 663, 737, 839
HaeIII GGCC 2 cut(s) 945, 2691
HapII CCGG 4 cut(s) 533, 1822, 1957, 2600
HgaI GACGC 2 cut(s) 2198, 2249
HhaI GCGC 1 cut(s) 2093
Hin6I GCGC 1 cut(s) 2091
HinP1I GCGC 1 cut(s) 2091
HincII GTYRAC 2 cut(s) 393, 402
HindII GTYRAC 2 cut(s) 393, 402
HindIII AAGCTT 4 cut(s) 116, 173, 1218, 1571
HinfI GANTC 5 cut(s) 149, 755, 1088, 2433, 2506
HpaI GTTAAC 2 cut(s) 393, 402
HpaII CCGG 4 cut(s) 533, 1822, 1957, 2600
HphI GGTGA 8 cut(s) 113, 288, 709, 1285, 1343, 1421, 2025, 2546
Hpy166II GTNNAC 9 cut(s) 393, 402, 666, 861, 1272, 1283, 1300, 1758, 2331
Hpy8I GTNNAC 9 cut(s) 393, 402, 666, 861, 1272, 1283, 1300, 1758, 2331
Hpy99I CGWCG 1 cut(s) 2243
HpyCH4III ACNGT 8 cut(s) 280, 390, 697, 706, 1269, 1297, 1597, 2306
HpyCH4IV ACGT 3 cut(s) 556, 2333, 2628
HpyF10VI GCNNNNNNNGC 7 cut(s) 425, 791, 938, 967, 2168, 2177, 2605
HpyF3I CTNAG 8 cut(s) 55, 315, 570, 1608, 1712, 2000, 2286, 2429
HpySE526I ACGT 3 cut(s) 556, 2333, 2628
HspAI GCGC 1 cut(s) 2091
KflI GGGWCCC 1 cut(s) 2265
Ksp22I TGATCA 1 cut(s) 1723
KspAI GTTAAC 2 cut(s) 393, 402
Kzo9I GATC 9 cut(s) 217, 435, 1144, 1723, 1930, 2050, 2207, 2289, 2470
LmnI GCTCC 7 cut(s) 346, 730, 1127, 1535, 2287, 2497, 2528
Lsp1109I GCAGC 9 cut(s) 364, 944, 1228, 1540, 1868, 2075, 2146, 2179, 2302
MaeII ACGT 3 cut(s) 556, 2333, 2628
MaeIII GTNAC 1 cut(s) 1967
MalI GATC 9 cut(s) 219, 437, 1146, 1725, 1932, 2052, 2209, 2291, 2472
MboI GATC 9 cut(s) 217, 435, 1144, 1723, 1930, 2050, 2207, 2289, 2470
MfeI CAATTG 1 cut(s) 2448
MflI RGATCY 3 cut(s) 1930, 2050, 2470
MhlI GDGCHC 9 cut(s) 51, 668, 1540, 1693, 2100, 2115, 2485, 2519, 2578
MluCI AATT 7 cut(s) 27, 88, 273, 874, 1447, 2251, 2448
MmeI TCCRAC 3 cut(s) 708, 1816, 2042
MroXI GAANNNNTTC 2 cut(s) 1553, 1947
MslI CAYNNNNRTG 1 cut(s) 2038
MspA1I CMGCKG 3 cut(s) 1162, 1305, 2586
MspI CCGG 4 cut(s) 533, 1822, 1957, 2600
MspR9I CCNGG 5 cut(s) 533, 1287, 1541, 1800, 1958
MunI CAATTG 1 cut(s) 2448
MvaI CCWGG 3 cut(s) 1287, 1541, 1800
MwoI GCNNNNNNNGC 7 cut(s) 425, 791, 938, 967, 2168, 2177, 2605
NciI CCSGG 2 cut(s) 533, 1958
NdeII GATC 9 cut(s) 217, 435, 1144, 1723, 1930, 2050, 2207, 2289, 2470
NlaIV GGNNCC 9 cut(s) 531, 691, 1537, 2052, 2070, 2266, 2267, 2283, 2499
NmeAIII GCCGAG 1 cut(s) 2119
NmuCI GTSAC 1 cut(s) 1967
NspI RCATGY 3 cut(s) 1486, 1912, 2641
PasI CCCWGGG 1 cut(s) 1540
PciI ACATGT 1 cut(s) 1482
PdmI GAANNNNTTC 2 cut(s) 1553, 1947
PfeI GAWTC 5 cut(s) 149, 755, 1088, 2433, 2506
PflFI GACNNNGTC 1 cut(s) 595
PflMI CCANNNNNTGG 1 cut(s) 305
PfoI TCCNGGA 2 cut(s) 531, 1956
PmaCI CACGTG 1 cut(s) 557
PmlI CACGTG 1 cut(s) 557
Ppu21I YACGTR 1 cut(s) 557
PpuMI RGGWCCY 1 cut(s) 2265
PscI ACATGT 1 cut(s) 1482
PshBI ATTAAT 1 cut(s) 168
Psp124BI GAGCTC 1 cut(s) 2485
Psp5II RGGWCCY 1 cut(s) 2265
Psp6I CCWGG 3 cut(s) 1285, 1539, 1798
PspCI CACGTG 1 cut(s) 557
PspFI CCCAGC 5 cut(s) 50, 428, 659, 733, 835
PspGI CCWGG 3 cut(s) 1285, 1539, 1798
PspN4I GGNNCC 9 cut(s) 531, 691, 1537, 2052, 2070, 2266, 2267, 2283, 2499
PspPI GGNCC 6 cut(s) 482, 529, 943, 2069, 2247, 2265
PspPPI RGGWCCY 1 cut(s) 2265
PstI CTGCAG 1 cut(s) 2320
PsuI RGATCY 3 cut(s) 1930, 2050, 2470
PsyI GACNNNGTC 1 cut(s) 595
PvuII CAGCTG 2 cut(s) 1162, 1305
RseI CAYNNNNRTG 1 cut(s) 2038
SacI GAGCTC 1 cut(s) 2485
Sau3AI GATC 9 cut(s) 217, 435, 1144, 1723, 1930, 2050, 2207, 2289, 2470
Sau96I GGNCC 6 cut(s) 482, 529, 943, 2069, 2247, 2265
ScaI AGTACT 2 cut(s) 1901, 2276
ScrFI CCNGG 5 cut(s) 533, 1287, 1541, 1800, 1958
SduI GDGCHC 9 cut(s) 51, 668, 1540, 1693, 2100, 2115, 2485, 2519, 2578
SfcI CTRYAG 2 cut(s) 153, 2316
SinI GGWCC 5 cut(s) 482, 529, 2069, 2247, 2265
SmiMI CAYNNNNRTG 1 cut(s) 2038
SmlI CTYRAG 2 cut(s) 1255, 2199
SmoI CTYRAG 2 cut(s) 1255, 2199
SpeI ACTAGT 1 cut(s) 1460
Sse9I AATT 7 cut(s) 27, 88, 273, 874, 1447, 2251, 2448
SsiI CCGC 9 cut(s) 14, 182, 455, 480, 941, 1030, 2163, 2584, 2597
SspI AATATT 1 cut(s) 65
SstI GAGCTC 1 cut(s) 2485
StyD4I CCNGG 5 cut(s) 531, 1285, 1539, 1798, 1956
StyI CCWWGG 6 cut(s) 207, 299, 360, 1405, 1685, 2144
TaaI ACNGT 8 cut(s) 280, 390, 697, 706, 1269, 1297, 1597, 2306
TaiI ACGT 3 cut(s) 559, 2336, 2631
TaqI TCGA 4 cut(s) 82, 628, 1143, 1487
TaqII GACCGA 1 cut(s) 100
TasI AATT 7 cut(s) 27, 88, 273, 874, 1447, 2251, 2448
TatI WGTACW 9 cut(s) 447, 673, 1151, 1264, 1424, 1619, 1899, 2075, 2274
TauI GCSGC 2 cut(s) 185, 2165
TfiI GAWTC 5 cut(s) 149, 755, 1088, 2433, 2506
TscAI CASTG 1 cut(s) 285
TseFI GTSAC 1 cut(s) 1967
TseI GCWGC 9 cut(s) 377, 932, 1241, 1528, 1856, 2088, 2159, 2192, 2315
Tsp45I GTSAC 1 cut(s) 1967
TspGWI ACGGA 3 cut(s) 1502, 2534, 2541
TspRI CASTG 1 cut(s) 285
Tth111I GACNNNGTC 1 cut(s) 595
Van91I CCANNNNNTGG 1 cut(s) 305
VneI GTGCAC 1 cut(s) 664
VpaK11BI GGWCC 5 cut(s) 482, 529, 2069, 2247, 2265
VspI ATTAAT 1 cut(s) 168
XapI RAATTY 1 cut(s) 88
XbaI TCTAGA 1 cut(s) 739
XceI RCATGY 3 cut(s) 1486, 1912, 2641
XcmI CCANNNNNNNNNTGG 1 cut(s) 1645
XmaJI CCTAGG 1 cut(s) 2144
XmiI GTMKAC 1 cut(s) 1299
XmnI GAANNNNTTC 2 cut(s) 1553, 1947
ZrmI AGTACT 2 cut(s) 1901, 2276
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.