MD12G1021500.v1.1

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0005763
Physical Location & Seq
Reverse (-)
2161242 .. 2165732
4491 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1021500.v1.1.491

Sequence Viewer

Length: 2727 bp
ATGGGTTCATTCGCCGGTACATCTGAAATTGTGGAAGCGAAAGATGAGCTGACTACAAGTCAATATTTTCACGGAATACATCAGCTGAATTCTGGATTTGGTAAAGATGACAAAGATTGGAAGCTTCCTCCTGTTCTAAAACTGGGATACAAGGATTCTCTAGAAGATGATATTAATAAGCTCTTCGAGGCAATCAAAACTCCATCCAAGGGTTCAGATCATTCAAAGCAAGTAGGCACTAGCCCTTTAAATAGAAGTGCCTTAAAGAAACCAATTACAGTGGGTGTGTCTCGCTCACCAGGGATTGGTAATTCAGAACCGGCGACACTGAAGCAGGCGTTAAGAGAGCTCTCTATTACCAAGGCATCAGAAATGGCTGCTATGAAACGATTATCAAAATTGACAAGTCCTGCAAGACCCTCGGAAGCTGGGAGGATCAAGACGTTATACAATGCAGTTGTTGTTCAATCCAGTCAATCTGGTGCCTCCTCAAGTGAAGATAGGGGAAATATGGTTGAAATCTCTCTGGTGCCAGAAGAAAGCATTTCAAATTCTTGTGAGGAGTTGTCTCAGCATCCTCAAATATCCAAGATGAAGTCATCAAGCCAGAGTGGACATTCTTCTCCTCGATTTGCCATTGGGAAAACAGAAAACAACTCTGGGAGCACAATAGTGCAAAATGAAAGTACTTCCAGTTGCAAGTTAAGGACAGTAGGGGAGCAAACAGTGAAGGCGGAACTGGGACAGAAGAAGAAAAATAAATCTAAAAGTGATCATATTTCAGGACTTGCTGAAAATGTTCCTTCCAGAACCATGTTACAGACAAAAACTAAGACGGAGCTGGTGAAGAAAGAGAAACTTAAATCTACAAGTGGCAATACATCAGTACTTGTTCAAAATGTTCCTGCCAAAACCAAGTTAGCTAATAAGCTATCAGCAGCAAAACCAGGGCGGAAAGTCAAATTGCATGTAGTACCTTCTTCATCGAGTTCGGTGAATCGAAATCCGGCAAGCAAAGTATCCAGAAATGCCCTCCGCTCTGTAAAAGCAGCCAGCAGGAATAGGAACATCGTAAAGAAGAAGACAAGGCAAGAGTCAAGTTCTGCGGCCTGCAACTCTAGTAATTATAATGAAGTTGATGCTGATTTTGATCCTTGTACAAGTCAGCTGGTCTGCGAGAGATGTCAATGTGCAATACAAGGAGCTGGACAACAATCCAATCAAGGTTCTTTGGCACCCCAGTTTAACGGTCTTACTGCTGAGGTAAACTCAAATAATGGGCAAAGCATTCCAGGTAAAGCAGGCTTCACCTTCGACAGCAGTAATACTGGCAATAAAGGGGGAGATGACATTATGAAAGCAAAAAAGAACCCCAAATCAAAAGAAAAAGGGGAATTCTCCCAAAGCTCAAAAAGTAGCGAAGGTGATCATAGTAGTAGTACGAGTTTGAGTGACGAGAGCAATGCCAGTGGATCTAGTTGCAGCAATAAGCCTCACATGTCGAAGGATGTGAGATGGGAGGCCATCCGTCATGTTCGGTTGCAGCATGGATCCCTGGGCTTGAGACACTTCAATCTTTTGAAGAAGCTTGGTTGTGGAGACATAGGAACCGTATATCTTGCTGAGCTCATTAGTACGAATTGCCTTTTTGCCATAAAGGTAATGGACAATGAGTTCTTGGCTAGAAGGAAGAAGATGCCTAGGGCGCAGACCGAAAGCGAAATACTGAGGATGCTTGATCATCCTTTTCTCCCTACACTGTATACCCAATTTACATCAGATAATTTGTCATGCTTAGTTATGGAGTATTGCCCAGGTGGAGATCTTCATGTCCTCCGGCAGAAGCAAATTGGAAGGTGTTTTTCTGAAGACGCAACAAGGTTCTATGTTGCGGAAGTTCTCCTTGCTTTGGAGTATTTACACATGCTTGGGGTAGTTTATCGGGATTTGAAACCAGAAAACATTCTAGTCCGGGAAGATGGTCACATCATGCTCACAGATTTTGACCTGTCTCTCAGGTGCACTGTCAGTCCAACTCTATTGAAATCACCTTCATGCGATGGTGATCGTGCAAGATTGTCTGGTCCATGTACACAATCTAGCTGCGCTGAGCCGTTCTGCATTGAACCCTCCTGTCAAGTCTCATGCTTCACCCCTAGGTTTTTACCGGCTGCTACAAAAACAAAGAAGTCCAAAAATGACCCTGCATCCCATGTTAGATCATTGCCGCAGCTTGTGGCAGAGCCTACTGATGCGCGGTCCAATTCCTTTGTCGGGACCCATGAGTACTTGGCTCCGGAGATCATCAAAGGAGAGGGTCATGGAGCTGCAGTTGATTGGTGGACGTTCGGAATCTTCCTTTACGAGCTTCTATATGGCAGAACACCGTTCAAGGGTATTAACAACGAGGAAACGTTGGCCAATGTGGTGTTGCAGAGCCTAAGGTTCCCAGAGGGCCCCCTTGTTAGTTTCCAGGCTCGAGATCTCATCAGAGGTCTGTTGGTAAAGGAGCCAGAGAATCGGTTGGGATCAGAGAAAGGGGCCGGTGAGATCAAGCAGCATCCGTTCTTTGAGGGCCTGAATTGGGCACTGATACGCTGCGCCATTCCGCCAGAACTGCCCGAGTTTTGTGATTTTGAAATTCCCGACATGCCTGCTGCTCAGGAGAATACCAAGTATTTGGAGTTAACAGCGACGGGAGAGCACCTAGAGTTTGAGTTATTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

909

Amino Acids

99.25

Weight (kDa)

8.81

Isoelectric Point (pI)

52.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 524 - 675 3.3e-36 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 525 - 673 2.4e-19 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 754 - 857 1.5e-20 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 761 - 841 3.5e-07 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1128
AccB1I GGYRCC 3 cut(s) 482, 529, 1234
AccB7I CCANNNNNTGG 1 cut(s) 305
AccBSI CCGCTC 1 cut(s) 1038
AccI GTMKAC 1 cut(s) 1763
AccII CGCG 1 cut(s) 2257
AccIII TCCGGA 1 cut(s) 2296
AciI CCGC 8 cut(s) 734, 952, 1036, 1106, 1892, 2228, 2257, 2609
AclI AACGTT 1 cut(s) 2414
AclWI GGATC 6 cut(s) 443, 1145, 1480, 1545, 1558, 2536
AcoI YGGCCR 1 cut(s) 2418
AcsI RAATTY 4 cut(s) 88, 550, 1394, 2640
AcuI CTGAAG 2 cut(s) 350, 1887
AfaI GTAC 9 cut(s) 19, 688, 888, 975, 1159, 1441, 1636, 2092, 2288
AfiI CCNNNNNNNGG 6 cut(s) 209, 305, 1909, 2274, 2393, 2584
AflIII ACRYGT 1 cut(s) 1497
AhdI GACNNNNNGTC 1 cut(s) 57
AjnI CCWGG 6 cut(s) 298, 946, 1291, 1554, 1813, 2472
AleI CACNNNNGTG 1 cut(s) 671
AloI GAACNNNNNNTCC 2 cut(s) 1658, 1690
Alw21I GWGCWC 5 cut(s) 351, 668, 1629, 2024, 2706
Alw26I GTCTC 6 cut(s) 294, 573, 1558, 1593, 2016, 2146
Alw44I GTGCAC 1 cut(s) 2020
AlwI GGATC 6 cut(s) 443, 1145, 1480, 1545, 1558, 2536
Ama87I CYCGRG 2 cut(s) 2478, 2621
Aor13HI TCCGGA 1 cut(s) 2296
AoxI GGCC 6 cut(s) 1107, 1521, 2418, 2455, 2541, 2575
ApaI GGGCCC 1 cut(s) 2459
ApaLI GTGCAC 1 cut(s) 2020
ApoI RAATTY 4 cut(s) 88, 550, 1394, 2640
AseI ATTAAT 1 cut(s) 174
Asp700I GAANNNNTTC 2 cut(s) 798, 1962
AspA2I CCTAGG 2 cut(s) 1700, 2156
AspLEI GCGC 4 cut(s) 1708, 2108, 2257, 2603
AspS9I GGNCC 7 cut(s) 2084, 2259, 2277, 2455, 2456, 2541, 2575
AsuC2I CCSGG 1 cut(s) 1973
AsuHPI GGTGA 9 cut(s) 288, 856, 1006, 1300, 1436, 2040, 2075, 2143, 2558
AvaI CYCGRG 2 cut(s) 2478, 2621
AvaII GGWCC 3 cut(s) 2084, 2259, 2277
AvrII CCTAGG 2 cut(s) 1700, 2156
AxyI CCTNAGG 1 cut(s) 2441
BaeGI GKGCMC 3 cut(s) 2024, 2459, 2590
BalI TGGCCA 1 cut(s) 2420
BamHI GGATCC 1 cut(s) 1550
BanI GGYRCC 3 cut(s) 482, 529, 1234
BanII GRGCYC 3 cut(s) 351, 1629, 2459
BbsI GAAGAC 2 cut(s) 1088, 1875
Bbv12I GWGCWC 5 cut(s) 351, 668, 1629, 2024, 2706
BbvCI CCTCAGC 1 cut(s) 1260
BccI CCATC 5 cut(s) 211, 1509, 1532, 1973, 2054
BceAI ACGGC 1 cut(s) 2098
BcgI CGANNNNNNTGC 6 cut(s) 402, 436, 1445, 1479, 2636, 2670
BciT130I CCWGG 6 cut(s) 300, 948, 1293, 1556, 1815, 2474
BciVI GTATCC 2 cut(s) 140, 1030
BclI TGATCA 3 cut(s) 772, 1426, 1738
BcnI CCSGG 1 cut(s) 1973
BcoDI GTCTC 6 cut(s) 294, 573, 1558, 1593, 2016, 2146
BfmI CTRYAG 1 cut(s) 2328
BfuI GTATCC 2 cut(s) 140, 1030
BglII AGATCT 2 cut(s) 1822, 2482
BlnI CCTAGG 2 cut(s) 1700, 2156
BlpI GCTNAGC 2 cut(s) 1623, 2109
BmcAI AGTACT 3 cut(s) 688, 888, 2288
Bme1390I CCNGG 7 cut(s) 300, 948, 1293, 1556, 1815, 1973, 2474
Bme18I GGWCC 3 cut(s) 2084, 2259, 2277
BmeRI GACNNNNNGTC 1 cut(s) 57
BmeT110I CYCGRG 2 cut(s) 2478, 2621
BmgT120I GGNCC 7 cut(s) 2084, 2259, 2277, 2455, 2456, 2541, 2575
BmrFI CCNGG 7 cut(s) 300, 948, 1293, 1556, 1815, 1973, 2474
BmrI ACTGGG 3 cut(s) 152, 749, 1234
BmsI GCATC 8 cut(s) 374, 583, 1129, 1686, 1722, 2216, 2242, 2569
BmuI ACTGGG 3 cut(s) 152, 749, 1234
BpiI GAAGAC 2 cut(s) 1088, 1875
BplI GAGNNNNNCTC 2 cut(s) 1253, 1285
Bpu10I CCTNAGC 2 cut(s) 1260, 2661
Bpu1102I GCTNAGC 2 cut(s) 1623, 2109
BpuEI CTTGAG 2 cut(s) 475, 1582
BpuMI CCSGG 1 cut(s) 1973
BsaBI GATNNNNATC 2 cut(s) 1149, 2064
BsaWI WCCGGW 1 cut(s) 2296
BsaXI ACNNNNNCTCC 2 cut(s) 2316, 2346
Bsc4I CCNNNNNNNGG 6 cut(s) 209, 305, 1909, 2274, 2393, 2584
Bse118I RCCGGY 4 cut(s) 14, 319, 2167, 2543
Bse1I ACTGG 7 cut(s) 147, 471, 693, 744, 1240, 1333, 1467
Bse21I CCTNAGG 1 cut(s) 2441
Bse3DI GCAATG 2 cut(s) 1468, 2222
Bse8I GATNNNNATC 2 cut(s) 1149, 2064
BseAI TCCGGA 1 cut(s) 2296
BseBI CCWGG 6 cut(s) 300, 948, 1293, 1556, 1815, 2474
BseGI GGATG 8 cut(s) 203, 574, 1513, 1524, 1737, 1741, 2207, 2560
BseJI GATNNNNATC 2 cut(s) 1149, 2064
BseLI CCNNNNNNNGG 6 cut(s) 209, 305, 1909, 2274, 2393, 2584
BseMI GCAATG 2 cut(s) 1468, 2222
BseMII CTCAG 7 cut(s) 584, 1251, 1614, 1718, 2029, 2100, 2675
BseNI ACTGG 7 cut(s) 147, 471, 693, 744, 1240, 1333, 1467
BseRI GAGGAG 3 cut(s) 478, 575, 615
BseSI GKGCMC 3 cut(s) 2024, 2459, 2590
BseYI CCCAGC 1 cut(s) 428
Bsh1236I CGCG 1 cut(s) 2257
BshFI GGCC 6 cut(s) 1109, 1523, 2420, 2457, 2543, 2577
BshNI GGYRCC 3 cut(s) 482, 529, 1234
BsiHKAI GWGCWC 5 cut(s) 351, 668, 1629, 2024, 2706
BsiHKCI CYCGRG 2 cut(s) 2478, 2621
BsiSI CCGG 8 cut(s) 15, 320, 1007, 1837, 1972, 2168, 2297, 2544
BslFI GGGAC 2 cut(s) 756, 2290
BslI CCNNNNNNNGG 6 cut(s) 209, 305, 1909, 2274, 2393, 2584
BsmAI GTCTC 6 cut(s) 294, 573, 1558, 1593, 2016, 2146
BsmFI GGGAC 2 cut(s) 756, 2290
BsmI GAATGC 1 cut(s) 1287
BsnI GGCC 6 cut(s) 1109, 1523, 2420, 2457, 2543, 2577
BsoBI CYCGRG 2 cut(s) 2478, 2621
Bsp120I GGGCCC 1 cut(s) 2455
Bsp1286I GDGCHC 7 cut(s) 351, 668, 1629, 2024, 2459, 2590, 2706
Bsp13I TCCGGA 1 cut(s) 2296
Bsp1407I TGTACA 2 cut(s) 1157, 2090
Bsp1720I GCTNAGC 2 cut(s) 1623, 2109
BspACI CCGC 8 cut(s) 734, 952, 1036, 1106, 1892, 2228, 2257, 2609
BspANI GGCC 6 cut(s) 1109, 1523, 2420, 2457, 2543, 2577
BspCNI CTCAG 7 cut(s) 583, 1252, 1615, 1719, 2028, 2101, 2674
BspEI TCCGGA 1 cut(s) 2296
BspFNI CGCG 1 cut(s) 2257
BspMAI CTGCAG 1 cut(s) 2332
BspPI GGATC 6 cut(s) 443, 1145, 1480, 1545, 1558, 2536
BspQI GCTCTTC 1 cut(s) 188
BspT107I GGYRCC 3 cut(s) 482, 529, 1234
BsrBI CCGCTC 1 cut(s) 1038
BsrDI GCAATG 2 cut(s) 1468, 2222
BsrFI RCCGGY 4 cut(s) 14, 319, 2167, 2543
BsrGI TGTACA 2 cut(s) 1157, 2090
BsrI ACTGG 7 cut(s) 147, 471, 693, 744, 1240, 1333, 1467
BssAI RCCGGY 4 cut(s) 14, 319, 2167, 2543
BssNAI GTATAC 1 cut(s) 1764
BssT1I CCWWGG 4 cut(s) 207, 360, 1700, 2156
Bst1107I GTATAC 1 cut(s) 1764
Bst2UI CCWGG 6 cut(s) 300, 948, 1293, 1556, 1815, 2474
Bst4CI ACNGT 8 cut(s) 280, 712, 727, 1250, 1612, 1761, 2026, 2388
Bst6I CTCTTC 1 cut(s) 188
BstAUI TGTACA 2 cut(s) 1157, 2090
BstC8I GCNNGC 6 cut(s) 336, 1012, 1054, 1111, 1303, 2655
BstF5I GGATG 8 cut(s) 203, 574, 1513, 1524, 1737, 1741, 2207, 2560
BstFNI CGCG 1 cut(s) 2257
BstHHI GCGC 4 cut(s) 1708, 2108, 2257, 2603
BstMAI GTCTC 6 cut(s) 294, 573, 1558, 1593, 2016, 2146
BstMWI GCNNNNNNNGC 2 cut(s) 1705, 2617
BstNI CCWGG 6 cut(s) 300, 948, 1293, 1556, 1815, 2474
BstNSI RCATGY 4 cut(s) 971, 1501, 1927, 2653
BstSCI CCNGG 7 cut(s) 298, 946, 1291, 1554, 1813, 1971, 2472
BstSFI CTRYAG 1 cut(s) 2328
BstSLI GKGCMC 3 cut(s) 2024, 2459, 2590
BstUI CGCG 1 cut(s) 2257
BstV2I GAAGAC 2 cut(s) 1088, 1875
BstX2I RGATCY 4 cut(s) 1472, 1550, 1822, 2482
BstXI CCANNNNNNTGG 1 cut(s) 2680
BstYI RGATCY 4 cut(s) 1472, 1550, 1822, 2482
BstZ17I GTATAC 1 cut(s) 1764
Bsu36I CCTNAGG 1 cut(s) 2441
BsuI GTATCC 2 cut(s) 140, 1030
BsuRI GGCC 6 cut(s) 1109, 1523, 2420, 2457, 2543, 2577
BtgZI GCGATG 1 cut(s) 2073
BtsCI GGATG 8 cut(s) 203, 574, 1513, 1524, 1737, 1741, 2207, 2560
BtsIMutI CAGTG 7 cut(s) 285, 326, 732, 1474, 1757, 2022, 2588
Cac8I GCNNGC 6 cut(s) 336, 1012, 1054, 1111, 1303, 2655
CfoI GCGC 4 cut(s) 1708, 2108, 2257, 2603
Cfr10I RCCGGY 4 cut(s) 14, 319, 2167, 2543
Cfr13I GGNCC 7 cut(s) 2084, 2259, 2277, 2455, 2456, 2541, 2575
CseI GACGC 1 cut(s) 1880
Csp6I GTAC 9 cut(s) 18, 687, 887, 974, 1158, 1440, 1635, 2091, 2287
CspCI CAANNNNNGTGG 4 cut(s) 261, 296, 1450, 1485
CviQI GTAC 9 cut(s) 18, 687, 887, 974, 1158, 1440, 1635, 2091, 2287
DraI TTTAAA 1 cut(s) 249
DriI GACNNNNNGTC 1 cut(s) 57
EaeI YGGCCR 1 cut(s) 2418
Eam1104I CTCTTC 1 cut(s) 188
Eam1105I GACNNNNNGTC 1 cut(s) 57
EarI CTCTTC 1 cut(s) 188
EciI GGCGGA 3 cut(s) 749, 967, 2598
Ecl136II GAGCTC 2 cut(s) 349, 1627
Eco130I CCWWGG 4 cut(s) 207, 360, 1700, 2156
Eco24I GRGCYC 3 cut(s) 351, 1629, 2459
Eco47I GGWCC 3 cut(s) 2084, 2259, 2277
Eco53kI GAGCTC 2 cut(s) 349, 1627
Eco57I CTGAAG 2 cut(s) 350, 1887
Eco81I CCTNAGG 1 cut(s) 2441
Eco88I CYCGRG 2 cut(s) 2478, 2621
EcoICRI GAGCTC 2 cut(s) 349, 1627
EcoO109I RGGNCCY 4 cut(s) 2277, 2455, 2456, 2575
EcoRI GAATTC 2 cut(s) 88, 1394
EcoRII CCWGG 6 cut(s) 298, 946, 1291, 1554, 1813, 2472
EcoT14I CCWWGG 4 cut(s) 207, 360, 1700, 2156
EcoT38I GRGCYC 3 cut(s) 351, 1629, 2459
ErhI CCWWGG 4 cut(s) 207, 360, 1700, 2156
FalI AAGNNNNNCTT 4 cut(s) 843, 875, 1887, 1919
FaqI GGGAC 2 cut(s) 756, 2290
FbaI TGATCA 3 cut(s) 772, 1426, 1738
FblI GTMKAC 1 cut(s) 1763
FokI GGATG 8 cut(s) 190, 561, 1511, 1520, 1728, 1744, 2194, 2547
FriOI GRGCYC 3 cut(s) 351, 1629, 2459
GlaI GCGC 4 cut(s) 1707, 2107, 2256, 2602
GsaI CCCAGC 1 cut(s) 432
HaeIII GGCC 6 cut(s) 1109, 1523, 2420, 2457, 2543, 2577
HapII CCGG 8 cut(s) 15, 320, 1007, 1837, 1972, 2168, 2297, 2544
HgaI GACGC 1 cut(s) 1880
HhaI GCGC 4 cut(s) 1708, 2108, 2257, 2603
Hin6I GCGC 4 cut(s) 1706, 2106, 2255, 2601
HinP1I GCGC 4 cut(s) 1706, 2106, 2255, 2601
HincII GTYRAC 1 cut(s) 2688
HindII GTYRAC 1 cut(s) 2688
HindIII AAGCTT 2 cut(s) 122, 1586
HinfI GANTC 5 cut(s) 155, 997, 1094, 2352, 2518
HpaI GTTAAC 1 cut(s) 2688
HpaII CCGG 8 cut(s) 15, 320, 1007, 1837, 1972, 2168, 2297, 2544
HphI GGTGA 9 cut(s) 288, 856, 1006, 1300, 1436, 2040, 2075, 2143, 2558
Hpy166II GTNNAC 7 cut(s) 614, 1267, 1764, 2022, 2093, 2343, 2688
Hpy8I GTNNAC 7 cut(s) 614, 1267, 1764, 2022, 2093, 2343, 2688
Hpy99I CGWCG 1 cut(s) 2698
HpyAV CCTTC 9 cut(s) 724, 813, 987, 1321, 1415, 1498, 1680, 1848, 2061
HpyCH4III ACNGT 8 cut(s) 280, 712, 727, 1250, 1612, 1761, 2026, 2388
HpyCH4IV ACGT 3 cut(s) 443, 2345, 2414
HpyF10VI GCNNNNNNNGC 2 cut(s) 1705, 2617
HpySE526I ACGT 3 cut(s) 443, 2345, 2414
HspAI GCGC 4 cut(s) 1706, 2106, 2255, 2601
KflI GGGWCCC 1 cut(s) 2277
Kpn2I TCCGGA 1 cut(s) 2296
Ksp22I TGATCA 3 cut(s) 772, 1426, 1738
KspAI GTTAAC 1 cut(s) 2688
LguI GCTCTTC 1 cut(s) 188
LmnI GCTCC 7 cut(s) 663, 718, 838, 1202, 2299, 2324, 2509
LweI GCATC 8 cut(s) 374, 583, 1129, 1686, 1722, 2216, 2242, 2569
MaeII ACGT 3 cut(s) 443, 2345, 2414
MaeIII GTNAC 3 cut(s) 816, 1451, 1982
MbiI CCGCTC 1 cut(s) 1038
MflI RGATCY 4 cut(s) 1472, 1550, 1822, 2482
MhlI GDGCHC 7 cut(s) 351, 668, 1629, 2024, 2459, 2590, 2706
MlsI TGGCCA 1 cut(s) 2420
MluNI TGGCCA 1 cut(s) 2420
MlyI GAGTC 1 cut(s) 1103
MmeI TCCRAC 1 cut(s) 2057
Mox20I TGGCCA 1 cut(s) 2420
MroI TCCGGA 1 cut(s) 2296
MroXI GAANNNNTTC 2 cut(s) 798, 1962
MscI TGGCCA 1 cut(s) 2420
MseI TTAA 9 cut(s) 174, 248, 263, 341, 704, 861, 1245, 2400, 2687
MslI CAYNNNNRTG 2 cut(s) 671, 2053
Msp20I TGGCCA 1 cut(s) 2420
MspA1I CMGCKG 2 cut(s) 85, 1168
MspI CCGG 8 cut(s) 15, 320, 1007, 1837, 1972, 2168, 2297, 2544
MspR9I CCNGG 7 cut(s) 300, 948, 1293, 1556, 1815, 1973, 2474
Mva1269I GAATGC 1 cut(s) 1287
MvaI CCWGG 6 cut(s) 300, 948, 1293, 1556, 1815, 2474
MvnI CGCG 1 cut(s) 2257
MwoI GCNNNNNNNGC 2 cut(s) 1705, 2617
NciI CCSGG 1 cut(s) 1973
NmuCI GTSAC 2 cut(s) 1451, 1982
NspI RCATGY 4 cut(s) 971, 1501, 1927, 2653
OliI CACNNNNGTG 1 cut(s) 671
PaeR7I CTCGAG 1 cut(s) 2478
PasI CCCWGGG 1 cut(s) 1555
PciI ACATGT 1 cut(s) 1497
PciSI GCTCTTC 1 cut(s) 188
PctI GAATGC 1 cut(s) 1287
PdmI GAANNNNTTC 2 cut(s) 798, 1962
PfeI GAWTC 4 cut(s) 155, 997, 2352, 2518
PflMI CCANNNNNTGG 1 cut(s) 305
PfoI TCCNGGA 1 cut(s) 1971
PleI GAGTC 1 cut(s) 1102
PpsI GAGTC 1 cut(s) 1102
PpuMI RGGWCCY 1 cut(s) 2277
PscI ACATGT 1 cut(s) 1497
PshBI ATTAAT 1 cut(s) 174
PsiI TTATAA 1 cut(s) 1128
Psp124BI GAGCTC 2 cut(s) 351, 1629
Psp1406I AACGTT 1 cut(s) 2414
Psp5II RGGWCCY 1 cut(s) 2277
Psp6I CCWGG 6 cut(s) 298, 946, 1291, 1554, 1813, 2472
PspFI CCCAGC 1 cut(s) 428
PspGI CCWGG 6 cut(s) 298, 946, 1291, 1554, 1813, 2472
PspOMI GGGCCC 1 cut(s) 2455
PspPI GGNCC 7 cut(s) 2084, 2259, 2277, 2455, 2456, 2541, 2575
PspPPI RGGWCCY 1 cut(s) 2277
PstI CTGCAG 1 cut(s) 2332
PsuI RGATCY 4 cut(s) 1472, 1550, 1822, 2482
PvuII CAGCTG 2 cut(s) 85, 1168
RsaI GTAC 9 cut(s) 19, 688, 888, 975, 1159, 1441, 1636, 2092, 2288
RsaNI GTAC 9 cut(s) 18, 687, 887, 974, 1158, 1440, 1635, 2091, 2287
RseI CAYNNNNRTG 2 cut(s) 671, 2053
SacI GAGCTC 2 cut(s) 351, 1629
SapI GCTCTTC 1 cut(s) 188
SaqAI TTAA 9 cut(s) 174, 248, 263, 341, 704, 861, 1245, 2400, 2687
Sau96I GGNCC 7 cut(s) 2084, 2259, 2277, 2455, 2456, 2541, 2575
ScaI AGTACT 3 cut(s) 688, 888, 2288
SchI GAGTC 1 cut(s) 1103
ScrFI CCNGG 7 cut(s) 300, 948, 1293, 1556, 1815, 1973, 2474
SduI GDGCHC 7 cut(s) 351, 668, 1629, 2024, 2459, 2590, 2706
SfaNI GCATC 8 cut(s) 374, 583, 1129, 1686, 1722, 2216, 2242, 2569
SfcI CTRYAG 1 cut(s) 2328
Sfr274I CTCGAG 1 cut(s) 2478
SinI GGWCC 3 cut(s) 2084, 2259, 2277
SlaI CTCGAG 1 cut(s) 2478
SmiMI CAYNNNNRTG 2 cut(s) 671, 2053
SmlI CTYRAG 3 cut(s) 490, 1561, 2478
SmoI CTYRAG 3 cut(s) 490, 1561, 2478
SsiI CCGC 8 cut(s) 734, 952, 1036, 1106, 1892, 2228, 2257, 2609
SspI AATATT 1 cut(s) 65
SstI GAGCTC 2 cut(s) 351, 1629
StyD4I CCNGG 7 cut(s) 298, 946, 1291, 1554, 1813, 1971, 2472
StyI CCWWGG 4 cut(s) 207, 360, 1700, 2156
TaaI ACNGT 8 cut(s) 280, 712, 727, 1250, 1612, 1761, 2026, 2388
TaiI ACGT 3 cut(s) 446, 2348, 2417
TaqI TCGA 7 cut(s) 186, 628, 986, 1000, 1314, 1502, 2479
TaqII GACCGA 1 cut(s) 1727
TatI WGTACW 5 cut(s) 686, 886, 1157, 2090, 2286
TauI GCSGC 2 cut(s) 1109, 2230
TfiI GAWTC 4 cut(s) 155, 997, 2352, 2518
Tru1I TTAA 9 cut(s) 174, 248, 263, 341, 704, 861, 1245, 2400, 2687
Tru9I TTAA 9 cut(s) 174, 248, 263, 341, 704, 861, 1245, 2400, 2687
TscAI CASTG 7 cut(s) 285, 333, 732, 1474, 1764, 2029, 2595
TseFI GTSAC 2 cut(s) 1451, 1982
Tsp45I GTSAC 2 cut(s) 1451, 1982
TspDTI ATGAA 8 cut(s) 398, 608, 696, 972, 1146, 1370, 1817, 2043
TspGWI ACGGA 4 cut(s) 87, 851, 1517, 2553
TspRI CASTG 7 cut(s) 285, 333, 732, 1474, 1764, 2029, 2595
Van91I CCANNNNNTGG 1 cut(s) 305
VneI GTGCAC 1 cut(s) 2020
VpaK11BI GGWCC 3 cut(s) 2084, 2259, 2277
VspI ATTAAT 1 cut(s) 174
XapI RAATTY 4 cut(s) 88, 550, 1394, 2640
XbaI TCTAGA 1 cut(s) 160
XceI RCATGY 4 cut(s) 971, 1501, 1927, 2653
XcmI CCANNNNNNNNNTGG 1 cut(s) 1660
XhoI CTCGAG 1 cut(s) 2478
XmaJI CCTAGG 2 cut(s) 1700, 2156
XmiI GTMKAC 1 cut(s) 1763
XmnI GAANNNNTTC 2 cut(s) 798, 1962
ZrmI AGTACT 3 cut(s) 688, 888, 2288
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.