Rroxscaffold_6G00408870

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0005763
Physical Location & Seq
Forward (+)
31389424 .. 31394250
4827 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00408870.1

Sequence Viewer

Length: 2715 bp
ATGGGTTCCTTTGCGGGTACTTCTGAGATTGTTGAAGCGACAGACGAGTCCAGTTCAGGTCAATATTCTCGTGGAACATATCAACCAAATACATGTTTGGGTGAGAATGATAGGAAGCTTCCTGCACTTAAACTAGGATCATCCAGGGATTCTATAGAAGATGATATTAATAAGCTTTTTGCGGCAATCCATCTCAAAGCTTCATCCAAGGGTTCAGATCATTCGAAGCAAGTAGGCACTAGCCCTTTGAGTAAAAATGCCTTAAAGAAGCCAATTACAATGGGTGTGTCTCACTCACCAAGGTTTGGGACTTCTGAGCCAGCGACTCTAAAGCAGGCATTAAGGGAGCTGTCTATTACCAAGGCATCGGAAATGGCTGCTATGAAACGGTTAACAAAGTTGACAAGTCCTTCAAGAGCCTCCGAAGCTGGGAGGATCAAGACATTGTACAATGCGGTTGTTGTTCAAGCCACTCAATGTGGTCCTTCCTCACATGAAGACAGGGGAAATATAGTTGAAATATCTCTGGTCCCAGAAGAAAGCACATCATATTCTCGTCAGAAGATGCCTGATCATCCTCCAATGCCCAAGACGAAGTCATCAAGCCAGAGTTCACATTCTTCTCCTCGATTTGTAAATGGGACAACAGGAACTAGTGCTGGGAGCACAGCAGTACAAAATGAAAGTGCTTCCACGTTGAGGACAGTTGGGGTACAAACAATAAAGTCGGAGTTGGGTCTGGAAGATAAAAATGAATCTGGTAGCGACCTTATTTCAGAAGTTGCTGAAAATTTACCTGCCAAAGCTGTTTTGAAGACAACATTAAAGGCAGAGCTGGGGAAGAAAGAAAAACATAAATCTACAAAGGAAAATAATTCAGTATCTGCTCGAAATAGTCCTACCAAAACCAAGTTAGCAAATAAGGTCTCAGCAGCAAAACCGGGGCGAAAAGTCAAGGTGCATGGAGTGCCTTCTTCATCTAGCTTCGTCAATGGAAATGTGGCAAGCAAGTTTTCCAGAAACACCCTCCGCAGTGTCAAACCATCCAGCAGGAACAAAAGTATTGTAAAGAAGAAGCAAAAACAAGATTCAATGTCTCCTTCCTGGAAAACTAGTAAGAATAATGAAGCTGATGCTGATATTGATGCATGTACAAGTCAGCTGGTCTGTGAAAGATGCCATATTGCCTTAAAAGGTGCCGGAAAACAATCCAATCAAGCTTTGGCAACCCCCAGTCTTGCTGCTGATATAAGCTCAAGCAATGTACGGTCCTGTCCAAGTAAACCAGGCTTCACTGTAGACAGCTGTAAGACCGGTAATACAGGTGGGGGTAATGTCGCAAAAGCAAAAAAGAACCCAAAATCAAAAGGAACAGGGGAGTTCTCCCAAAGCTCAAAAAGTAGCCAAGGTGAGTATAGTAGTAGTACAAGTTTGAGTGATGAGAGTAATTTAAGTAGGACTAGTTGTAGCAATAAACCTCACATGTCGAAGGACGTGAGGTGGGAAGCCATCCGTCATGTTCGGACGCAGTATGGAGCCCTGGGCTTGAGACACTTTAATCTTTTGAAGAAGCTTGGTTGTGGAGACATTGGAACTGTATATCTTGCTGAGCTAATCAGTACAAACTGCCTTTTCGCCATAAAGGTAATGGACAATGAGTTCCTGGCAAGAAGGAAGAAGATGCCAAGGGCTCACACAGAAAGTGAAATACTGAGGATGCTTGATCATCCTTTTCTCCCTACATTGTATTCCCAGTTTACATCAGATAATCTTTCATGTTTAGTAATGGAGTATTGTCCTGGTGGAGATCTTCATGTCCTCCGGCAGAAACAGGTCGGAAGGTGTTTTTCTGAAGCAGCTACAAGGTTTTATGTGGCTGAAGTCCTGCTTGCTTTGGAGTACTTGCACATGCTTGGTGTAGTTTATCGGGATCTGAAACCGGAAAACATTCTTGTCCGGGATGATGGTCACATAATGCTTACAGATTTTGACCTGTCTCTCAGATGCTCTGTCAGTCCAACTCTTTTGAAATCACCTTCATGTGATGCAGATCCTGCAAGAATGTCTGGTCCATGTACAGCATCTAACTGCGCTGAGCCCTTCTGCATTGAACCCTCCTCTTGTCAAGTCTCATGCTTCAGCCCTAGGTTTCTACCTGCTGCCGCAAAAACAAGGAAGTTGAAAAATGACCCTACATCTCAAGTCAGATCATTGCCACAGCTTGTGGCGGAGCCTAGCGACGCACGGTCCAATTCCTTTGTTGGGACCCACGAGTACTTGGCCCCCGAGATCATCAAAGGAGACGGTCATGGTGCTGCAGTTGATTGGTGGACGTTTGGAATCTTTCTTTACGAGCTTCTATATGGCAGAACACCGTTTAAGGGTGTTAATAACGAGGACACATTAGGCAATGTGGTCATGCAGAGCCTAAGATTCCCCGACACCCCGATTGTGAGCTTCCAGGCTAGGGATCTCATCAGAGGGCTGTTGGTAAAGGAGCCAGAGAATCGGTTGGGCACGGAGAAAGGAGCCGGTGAGATTAAGCAGCATCCATTCTTTGAAGGTTTGAATTGGGCACTAATCCGCTGTGCTGTTCCACCCGAACTGCCTGATTTTTGCGACTTTGAAGTTCCAGACATGCCTGCTCAGGACGATAACGCCAAGTACTTGGAGTTCAAAGCTACCGGAGAGCACCTAGAGTTTGAGTTATTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

904

Amino Acids

98.32

Weight (kDa)

9.0

Isoelectric Point (pI)

49.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 519 - 670 8.9e-36 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 520 - 669 3.4e-19 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 749 - 853 3.4e-20 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 757 - 838 4.7e-07 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 46
Acc36I ACCTGC 2 cut(s) 805, 2164
AccB1I GGYRCC 1 cut(s) 1196
AccB7I CCANNNNNTGG 1 cut(s) 305
AccI GTMKAC 1 cut(s) 1299
AciI CCGC 7 cut(s) 14, 182, 455, 1030, 2163, 2228, 2584
AclWI GGATC 5 cut(s) 145, 443, 1938, 2045, 2478
AcsI RAATTY 1 cut(s) 790
AcuI CTGAAG 3 cut(s) 1872, 1899, 2122
AfiI CCNNNNNNNGG 6 cut(s) 305, 429, 699, 2262, 2381, 2467
AflIII ACRYGT 2 cut(s) 92, 1482
AgeI ACCGGT 1 cut(s) 1313
AhdI GACNNNNNGTC 1 cut(s) 2245
AhlI ACTAGT 3 cut(s) 653, 1112, 1460
AjiI CACGTC 1 cut(s) 1495
AjnI CCWGG 7 cut(s) 143, 1103, 1285, 1539, 1662, 1798, 2460
AloI GAACNNNNNNTCC 2 cut(s) 1643, 1675
Alw21I GWGCWC 2 cut(s) 668, 2694
Alw26I GTCTC 8 cut(s) 294, 931, 1101, 1543, 1578, 2001, 2134, 2295
AlwI GGATC 5 cut(s) 145, 443, 1938, 2045, 2478
AlwNI CAGNNNCTG 2 cut(s) 884, 2054
Ama87I CYCGRG 1 cut(s) 2285
AoxI GGCC 1 cut(s) 2280
ApeKI GCWGC 7 cut(s) 377, 932, 1241, 1856, 2159, 2315, 2545
ApoI RAATTY 1 cut(s) 790
AseI ATTAAT 1 cut(s) 168
AsiGI ACCGGT 1 cut(s) 1313
Asp700I GAANNNNTTC 1 cut(s) 1947
AspA2I CCTAGG 1 cut(s) 2144
AspLEI GCGC 1 cut(s) 2093
AspS9I GGNCC 7 cut(s) 482, 529, 1269, 2069, 2247, 2265, 2281
AsuC2I CCSGG 2 cut(s) 942, 1958
AsuHPI GGTGA 5 cut(s) 113, 288, 1421, 2025, 2546
AsuII TTCGAA 1 cut(s) 224
AvaI CYCGRG 1 cut(s) 2285
AvaII GGWCC 6 cut(s) 482, 529, 1269, 2069, 2247, 2265
AvrII CCTAGG 1 cut(s) 2144
BaeGI GKGCMC 2 cut(s) 2519, 2578
BanI GGYRCC 1 cut(s) 1196
BanII GRGCYC 3 cut(s) 1540, 1693, 2100
BauI CACGAG 2 cut(s) 69, 2270
BbsI GAAGAC 2 cut(s) 504, 821
Bbv12I GWGCWC 2 cut(s) 668, 2694
BbvI GCAGC 7 cut(s) 364, 944, 1228, 1868, 2146, 2302, 2557
BccI CCATC 4 cut(s) 198, 1051, 1517, 1958
BciT130I CCWGG 7 cut(s) 145, 1105, 1287, 1541, 1664, 1800, 2462
BclI TGATCA 2 cut(s) 571, 1723
BcnI CCSGG 2 cut(s) 942, 1958
BcoDI GTCTC 8 cut(s) 294, 931, 1101, 1543, 1578, 2001, 2134, 2295
BcuI ACTAGT 3 cut(s) 653, 1112, 1460
BfmI CTRYAG 3 cut(s) 153, 1296, 2316
BfuAI ACCTGC 2 cut(s) 805, 2164
BglII AGATCT 1 cut(s) 1807
BisI GCNGC 9 cut(s) 183, 378, 933, 1242, 1857, 2160, 2163, 2316, 2546
BlnI CCTAGG 1 cut(s) 2144
BlpI GCTNAGC 2 cut(s) 1608, 2094
BlsI GCNGC 9 cut(s) 184, 379, 934, 1243, 1858, 2161, 2164, 2317, 2547
BmcAI AGTACT 3 cut(s) 1901, 2276, 2666
Bme1390I CCNGG 9 cut(s) 145, 942, 1105, 1287, 1541, 1664, 1800, 1958, 2462
Bme18I GGWCC 6 cut(s) 482, 529, 1269, 2069, 2247, 2265
BmeRI GACNNNNNGTC 1 cut(s) 2245
BmeT110I CYCGRG 1 cut(s) 2285
BmgBI CACGTC 1 cut(s) 1495
BmgT120I GGNCC 7 cut(s) 482, 529, 1269, 2069, 2247, 2265, 2281
BmrFI CCNGG 9 cut(s) 145, 942, 1105, 1287, 1541, 1664, 1800, 1958, 2462
BmrI ACTGGG 2 cut(s) 1227, 1747
BmuI ACTGGG 2 cut(s) 1227, 1747
BpiI GAAGAC 2 cut(s) 504, 821
Bpu10I CCTNAGC 1 cut(s) 2646
Bpu1102I GCTNAGC 2 cut(s) 1608, 2094
Bpu14I TTCGAA 1 cut(s) 224
BpuEI CTTGAG 3 cut(s) 1240, 1567, 2184
BpuMI CCSGG 2 cut(s) 942, 1958
BsaBI GATNNNNATC 1 cut(s) 2049
BsaI GGTCTC 1 cut(s) 931
BsaWI WCCGGW 3 cut(s) 1313, 1939, 2684
Bsc4I CCNNNNNNNGG 6 cut(s) 305, 429, 699, 2262, 2381, 2467
Bse118I RCCGGY 2 cut(s) 1313, 2531
Bse1I ACTGG 3 cut(s) 51, 1233, 1753
Bse3DI GCAATG 3 cut(s) 1267, 2210, 2416
Bse8I GATNNNNATC 1 cut(s) 2049
BseBI CCWGG 7 cut(s) 145, 1105, 1287, 1541, 1664, 1800, 2462
BseGI GGATG 9 cut(s) 140, 203, 574, 1043, 1509, 1722, 1726, 1966, 2548
BseJI GATNNNNATC 1 cut(s) 2049
BseLI CCNNNNNNNGG 6 cut(s) 305, 429, 699, 2262, 2381, 2467
BseMI GCAATG 3 cut(s) 1267, 2210, 2416
BseMII CTCAG 8 cut(s) 15, 306, 942, 1599, 1703, 2014, 2085, 2660
BseNI ACTGG 3 cut(s) 51, 1233, 1753
BseRI GAGGAG 2 cut(s) 615, 2107
BseSI GKGCMC 2 cut(s) 2519, 2578
BseXI GCAGC 7 cut(s) 364, 944, 1228, 1868, 2146, 2302, 2557
BseYI CCCAGC 3 cut(s) 428, 659, 835
BsgI GTGCAG 1 cut(s) 108
BshFI GGCC 1 cut(s) 2282
BshNI GGYRCC 1 cut(s) 1196
BshTI ACCGGT 1 cut(s) 1313
BsiHKAI GWGCWC 2 cut(s) 668, 2694
BsiHKCI CYCGRG 1 cut(s) 2285
BsiSI CCGG 8 cut(s) 941, 1200, 1314, 1822, 1940, 1957, 2532, 2685
BslFI GGGAC 4 cut(s) 322, 515, 655, 2278
BslI CCNNNNNNNGG 6 cut(s) 305, 429, 699, 2262, 2381, 2467
BsmAI GTCTC 8 cut(s) 294, 931, 1101, 1543, 1578, 2001, 2134, 2295
BsmBI CGTCTC 1 cut(s) 2295
BsmFI GGGAC 4 cut(s) 322, 515, 655, 2278
BsnI GGCC 1 cut(s) 2282
Bso31I GGTCTC 1 cut(s) 931
BsoBI CYCGRG 1 cut(s) 2285
Bsp119I TTCGAA 1 cut(s) 224
Bsp1286I GDGCHC 7 cut(s) 668, 1540, 1693, 2100, 2519, 2578, 2694
Bsp1407I TGTACA 3 cut(s) 447, 1151, 2075
Bsp1720I GCTNAGC 2 cut(s) 1608, 2094
BspACI CCGC 7 cut(s) 14, 182, 455, 1030, 2163, 2228, 2584
BspANI GGCC 1 cut(s) 2282
BspCNI CTCAG 8 cut(s) 16, 307, 941, 1600, 1704, 2013, 2086, 2659
BspMAI CTGCAG 1 cut(s) 2320
BspMI ACCTGC 2 cut(s) 805, 2164
BspPI GGATC 5 cut(s) 145, 443, 1938, 2045, 2478
BspT104I TTCGAA 1 cut(s) 224
BspT107I GGYRCC 1 cut(s) 1196
BspTNI GGTCTC 1 cut(s) 931
BsrDI GCAATG 3 cut(s) 1267, 2210, 2416
BsrFI RCCGGY 2 cut(s) 1313, 2531
BsrGI TGTACA 3 cut(s) 447, 1151, 2075
BsrI ACTGG 3 cut(s) 51, 1233, 1753
BssAI RCCGGY 2 cut(s) 1313, 2531
BssSI CACGAG 2 cut(s) 69, 2270
BssT1I CCWWGG 6 cut(s) 207, 299, 360, 1405, 1685, 2144
Bst2BI CACGAG 2 cut(s) 69, 2270
Bst2UI CCWGG 7 cut(s) 145, 1105, 1287, 1541, 1664, 1800, 2462
Bst4CI ACNGT 8 cut(s) 390, 706, 1269, 1297, 1597, 2247, 2306, 2376
BstAPI GCANNNNNTGC 2 cut(s) 967, 2054
BstAUI TGTACA 3 cut(s) 447, 1151, 2075
BstBI TTCGAA 1 cut(s) 224
BstC8I GCNNGC 5 cut(s) 321, 336, 1006, 1890, 2643
BstDEI CTNAG 9 cut(s) 24, 315, 928, 1608, 1712, 2000, 2094, 2429, 2646
BstF5I GGATG 9 cut(s) 140, 203, 574, 1043, 1509, 1722, 1726, 1966, 2548
BstHHI GCGC 1 cut(s) 2093
BstMAI GTCTC 8 cut(s) 294, 931, 1101, 1543, 1578, 2001, 2134, 2295
BstMWI GCNNNNNNNGC 3 cut(s) 425, 967, 2054
BstNI CCWGG 7 cut(s) 145, 1105, 1287, 1541, 1664, 1800, 2462
BstNSI RCATGY 5 cut(s) 96, 1152, 1486, 1912, 2641
BstSCI CCNGG 9 cut(s) 143, 940, 1103, 1285, 1539, 1662, 1798, 1956, 2460
BstSFI CTRYAG 3 cut(s) 153, 1296, 2316
BstSLI GKGCMC 2 cut(s) 2519, 2578
BstV1I GCAGC 7 cut(s) 364, 944, 1228, 1868, 2146, 2302, 2557
BstV2I GAAGAC 2 cut(s) 504, 821
BstX2I RGATCY 4 cut(s) 1807, 1930, 2050, 2470
BstXI CCANNNNNNTGG 1 cut(s) 2668
BstYI RGATCY 4 cut(s) 1807, 1930, 2050, 2470
BsuRI GGCC 1 cut(s) 2282
BtrI CACGTC 1 cut(s) 1495
BtsCI GGATG 9 cut(s) 140, 203, 574, 1043, 1509, 1722, 1726, 1966, 2548
BtsI GCAGTG 1 cut(s) 1039
BtsIMutI CAGTG 2 cut(s) 1039, 1293
BveI ACCTGC 2 cut(s) 805, 2164
Cac8I GCNNGC 5 cut(s) 321, 336, 1006, 1890, 2643
CaiI CAGNNNCTG 2 cut(s) 884, 2054
CfoI GCGC 1 cut(s) 2093
Cfr10I RCCGGY 2 cut(s) 1313, 2531
Cfr13I GGNCC 7 cut(s) 482, 529, 1269, 2069, 2247, 2265, 2281
CseI GACGC 2 cut(s) 1534, 2249
CspAI ACCGGT 1 cut(s) 1313
DdeI CTNAG 9 cut(s) 24, 315, 928, 1608, 1712, 2000, 2094, 2429, 2646
DrdI GACNNNNNNGTC 1 cut(s) 46
DriI GACNNNNNGTC 1 cut(s) 2245
DseDI GACNNNNNNGTC 1 cut(s) 46
Eam1105I GACNNNNNGTC 1 cut(s) 2245
EciI GGCGGA 1 cut(s) 2243
Eco130I CCWWGG 6 cut(s) 207, 299, 360, 1405, 1685, 2144
Eco24I GRGCYC 3 cut(s) 1540, 1693, 2100
Eco31I GGTCTC 1 cut(s) 931
Eco47I GGWCC 6 cut(s) 482, 529, 1269, 2069, 2247, 2265
Eco57I CTGAAG 3 cut(s) 1872, 1899, 2122
Eco88I CYCGRG 1 cut(s) 2285
EcoO109I RGGNCCY 1 cut(s) 2265
EcoRII CCWGG 7 cut(s) 143, 1103, 1285, 1539, 1662, 1798, 2460
EcoT14I CCWWGG 6 cut(s) 207, 299, 360, 1405, 1685, 2144
EcoT22I ATGCAT 1 cut(s) 1150
EcoT38I GRGCYC 3 cut(s) 1540, 1693, 2100
ErhI CCWWGG 6 cut(s) 207, 299, 360, 1405, 1685, 2144
Esp3I CGTCTC 1 cut(s) 2295
FalI AAGNNNNNCTT 2 cut(s) 1872, 1904
FaqI GGGAC 4 cut(s) 322, 515, 655, 2278
FauI CCCGC 1 cut(s) 7
FbaI TGATCA 2 cut(s) 571, 1723
FblI GTMKAC 1 cut(s) 1299
Fnu4HI GCNGC 9 cut(s) 183, 378, 933, 1242, 1857, 2160, 2163, 2316, 2546
FokI GGATG 9 cut(s) 127, 190, 561, 1030, 1496, 1713, 1729, 1973, 2535
FriOI GRGCYC 3 cut(s) 1540, 1693, 2100
Fsp4HI GCNGC 9 cut(s) 183, 378, 933, 1242, 1857, 2160, 2163, 2316, 2546
GlaI GCGC 1 cut(s) 2092
GluI GCNGC 9 cut(s) 183, 378, 933, 1242, 1857, 2160, 2163, 2316, 2546
GsaI CCCAGC 3 cut(s) 432, 663, 839
HaeIII GGCC 1 cut(s) 2282
HapII CCGG 8 cut(s) 941, 1200, 1314, 1822, 1940, 1957, 2532, 2685
HgaI GACGC 2 cut(s) 1534, 2249
HhaI GCGC 1 cut(s) 2093
Hin6I GCGC 1 cut(s) 2091
HinP1I GCGC 1 cut(s) 2091
HincII GTYRAC 2 cut(s) 393, 402
HindII GTYRAC 2 cut(s) 393, 402
HindIII AAGCTT 5 cut(s) 116, 173, 198, 1218, 1571
HinfI GANTC 8 cut(s) 47, 149, 325, 755, 1088, 2340, 2433, 2506
HpaI GTTAAC 1 cut(s) 393
HpaII CCGG 8 cut(s) 941, 1200, 1314, 1822, 1940, 1957, 2532, 2685
HphI GGTGA 5 cut(s) 113, 288, 1421, 2025, 2546
Hpy166II GTNNAC 7 cut(s) 393, 402, 614, 1283, 1300, 1758, 2331
Hpy188III TCNNGA 7 cut(s) 414, 439, 740, 1017, 1928, 2633, 2648
Hpy8I GTNNAC 7 cut(s) 393, 402, 614, 1283, 1300, 1758, 2331
Hpy99I CGWCG 1 cut(s) 2243
HpyCH4III ACNGT 8 cut(s) 390, 706, 1269, 1297, 1597, 2247, 2306, 2376
HpyCH4IV ACGT 3 cut(s) 695, 1494, 2333
HpyCH4V TGCA 9 cut(s) 125, 961, 1148, 1906, 2048, 2057, 2106, 2318, 2422
HpyF10VI GCNNNNNNNGC 3 cut(s) 425, 967, 2054
HpyF3I CTNAG 9 cut(s) 24, 315, 928, 1608, 1712, 2000, 2094, 2429, 2646
HpySE526I ACGT 3 cut(s) 695, 1494, 2333
HspAI GCGC 1 cut(s) 2091
KflI GGGWCCC 1 cut(s) 2265
Ksp22I TGATCA 2 cut(s) 571, 1723
KspAI GTTAAC 1 cut(s) 393
LmnI GCTCC 6 cut(s) 346, 663, 1535, 2230, 2497, 2528
Lsp1109I GCAGC 7 cut(s) 364, 944, 1228, 1868, 2146, 2302, 2557
MaeII ACGT 3 cut(s) 695, 1494, 2333
MaeIII GTNAC 1 cut(s) 1967
MflI RGATCY 4 cut(s) 1807, 1930, 2050, 2470
MhlI GDGCHC 7 cut(s) 668, 1540, 1693, 2100, 2519, 2578, 2694
MluCI AATT 6 cut(s) 273, 790, 874, 1447, 2251, 2569
MlyI GAGTC 2 cut(s) 56, 319
MmeI TCCRAC 3 cut(s) 708, 1816, 2042
Mph1103I ATGCAT 1 cut(s) 1150
MroXI GAANNNNTTC 1 cut(s) 1947
MslI CAYNNNNRTG 1 cut(s) 2038
MspA1I CMGCKG 3 cut(s) 1162, 1305, 2586
MspI CCGG 8 cut(s) 941, 1200, 1314, 1822, 1940, 1957, 2532, 2685
MspR9I CCNGG 9 cut(s) 145, 942, 1105, 1287, 1541, 1664, 1800, 1958, 2462
MvaI CCWGG 7 cut(s) 145, 1105, 1287, 1541, 1664, 1800, 2462
MwoI GCNNNNNNNGC 3 cut(s) 425, 967, 2054
NciI CCSGG 2 cut(s) 942, 1958
NmuCI GTSAC 1 cut(s) 1967
NsiI ATGCAT 1 cut(s) 1150
NspI RCATGY 5 cut(s) 96, 1152, 1486, 1912, 2641
NspV TTCGAA 1 cut(s) 224
PasI CCCWGGG 1 cut(s) 1540
PciI ACATGT 2 cut(s) 92, 1482
PdmI GAANNNNTTC 1 cut(s) 1947
PfeI GAWTC 6 cut(s) 149, 755, 1088, 2340, 2433, 2506
PflFI GACNNNGTC 1 cut(s) 595
PflMI CCANNNNNTGG 1 cut(s) 305
PfoI TCCNGGA 2 cut(s) 1103, 1956
PinAI ACCGGT 1 cut(s) 1313
PkrI GCNGC 9 cut(s) 184, 379, 934, 1243, 1858, 2161, 2164, 2317, 2547
PleI GAGTC 2 cut(s) 55, 319
PpsI GAGTC 2 cut(s) 55, 319
PpuMI RGGWCCY 1 cut(s) 2265
PscI ACATGT 2 cut(s) 92, 1482
PshBI ATTAAT 1 cut(s) 168
Psp5II RGGWCCY 1 cut(s) 2265
Psp6I CCWGG 7 cut(s) 143, 1103, 1285, 1539, 1662, 1798, 2460
PspFI CCCAGC 3 cut(s) 428, 659, 835
PspGI CCWGG 7 cut(s) 143, 1103, 1285, 1539, 1662, 1798, 2460
PspPI GGNCC 7 cut(s) 482, 529, 1269, 2069, 2247, 2265, 2281
PspPPI RGGWCCY 1 cut(s) 2265
PsrI GAACNNNNNNTAC 2 cut(s) 2657, 2689
PstI CTGCAG 1 cut(s) 2320
PstNI CAGNNNCTG 2 cut(s) 884, 2054
PsuI RGATCY 4 cut(s) 1807, 1930, 2050, 2470
PsyI GACNNNGTC 1 cut(s) 595
PvuII CAGCTG 2 cut(s) 1162, 1305
RseI CAYNNNNRTG 1 cut(s) 2038
SatI GCNGC 9 cut(s) 183, 378, 933, 1242, 1857, 2160, 2163, 2316, 2546
Sau96I GGNCC 7 cut(s) 482, 529, 1269, 2069, 2247, 2265, 2281
ScaI AGTACT 3 cut(s) 1901, 2276, 2666
SchI GAGTC 2 cut(s) 56, 319
ScrFI CCNGG 9 cut(s) 145, 942, 1105, 1287, 1541, 1664, 1800, 1958, 2462
SduI GDGCHC 7 cut(s) 668, 1540, 1693, 2100, 2519, 2578, 2694
SfcI CTRYAG 3 cut(s) 153, 1296, 2316
SfuI TTCGAA 1 cut(s) 224
SinI GGWCC 6 cut(s) 482, 529, 1269, 2069, 2247, 2265
SmiMI CAYNNNNRTG 1 cut(s) 2038
SmlI CTYRAG 3 cut(s) 1255, 1546, 2199
SmoI CTYRAG 3 cut(s) 1255, 1546, 2199
SpeI ACTAGT 3 cut(s) 653, 1112, 1460
Sse9I AATT 6 cut(s) 273, 790, 874, 1447, 2251, 2569
SsiI CCGC 7 cut(s) 14, 182, 455, 1030, 2163, 2228, 2584
SspI AATATT 1 cut(s) 65
StyD4I CCNGG 9 cut(s) 143, 940, 1103, 1285, 1539, 1662, 1798, 1956, 2460
StyI CCWWGG 6 cut(s) 207, 299, 360, 1405, 1685, 2144
TaaI ACNGT 8 cut(s) 390, 706, 1269, 1297, 1597, 2247, 2306, 2376
TaiI ACGT 3 cut(s) 698, 1497, 2336
TaqI TCGA 4 cut(s) 224, 628, 889, 1487
TasI AATT 6 cut(s) 273, 790, 874, 1447, 2251, 2569
TatI WGTACW 9 cut(s) 447, 673, 1151, 1424, 1619, 1899, 2075, 2274, 2664
TauI GCSGC 2 cut(s) 185, 2165
TfiI GAWTC 6 cut(s) 149, 755, 1088, 2340, 2433, 2506
TscAI CASTG 2 cut(s) 1039, 1300
TseFI GTSAC 1 cut(s) 1967
TseI GCWGC 7 cut(s) 377, 932, 1241, 1856, 2159, 2315, 2545
Tsp45I GTSAC 1 cut(s) 1967
TspGWI ACGGA 2 cut(s) 1502, 2534
TspRI CASTG 2 cut(s) 1039, 1300
Tth111I GACNNNGTC 1 cut(s) 595
Van91I CCANNNNNTGG 1 cut(s) 305
VpaK11BI GGWCC 6 cut(s) 482, 529, 1269, 2069, 2247, 2265
VspI ATTAAT 1 cut(s) 168
XapI RAATTY 1 cut(s) 790
XceI RCATGY 5 cut(s) 96, 1152, 1486, 1912, 2641
XcmI CCANNNNNNNNNTGG 2 cut(s) 1219, 1645
XmaJI CCTAGG 1 cut(s) 2144
XmiI GTMKAC 1 cut(s) 1299
XmnI GAANNNNTTC 1 cut(s) 1947
ZrmI AGTACT 3 cut(s) 1901, 2276, 2666
Zsp2I ATGCAT 1 cut(s) 1150
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.