MD14G1018600.v1.1

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0005763
Physical Location & Seq
Reverse (-)
1748282 .. 1752726
4445 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1018600.v1.1.491

Sequence Viewer

Length: 2649 bp
ATGGCTTCGTCTTCTGGTACTTCTGAAATTGTTGAAGCAAAAGACGAGCTAATTACAGGTCAATATTCTCACGGAATACATCGAACGAATCCTGGATTGAGTAGGGGTGAGAAAGATCGGAAGCTTCCTCCTGTTCTAAAACTGGGATACAAGGATTCTCTAGAAGATGATATTAATAAGCTCTTTGAGGCAATCAAAACATCATCCAAGGGTTCAGATCGTTCAAAGCAAGTAGGCACTAGCCCTTTAAATAGAAATGCCTTAAAGAAACCAATTACAGTGGGTGTGTCTCACTCACCAGGGATTGGGAATTCAGAACCGGCGACTCTGAAGCAGGCGTTAAGAGAGCTCTCTATTACCAAGGCATCAGAAATGGCTGCTATGAAACGGTTATCAAAATTGACAAGTCCTTCAAGAGCCTCGGAATCTGGGAGGATCAAGACGTTATACAATGCAGTTGTTGTTCAATCTAGTCACTCTGGCGCCTCCTCAAGTGAAGATAGGGGAAATATGGTTGAAATCTCTCTGGTGCCAGAAGAAAGCATTTCAAATTCTTGCAGGGAGCTGTCCGAGCATCCTCAAATGTCCAAGATGAAGTCATCAAGCCAGAGTGGACATTCTTCTCCTCGATTTGCCATTGGGAAAACAGAAAATAATTCCGGGAGCACAATAGTGCAAAATGAAAGTACTTCCAGTTGCAAGTTAAGGACAGTAGGGGAGCAAACAGTAAAGGTGGAAATGGGAAAGAAGGAGAACAATAAATCTACGAATGACCATATTTCAGAACTTGCTGAAAATGGCCCTTCCAACACCGTGTTACGGACAAAAACTAAGTCGGAGCTGGGGAAGAAAGAGAAACTTAAATCTACAAGTGGCAATACTTCAGGACTTGTTCAAAATGTTCCTGCCAAGACCAAGTTAGCGAATAAGGTATCAGCAGTAAAACCAGGGCGAAAAGTCAAGTTGCATGTAGTACCTTCTTCATCTAGTTTGGTGAATGTAAATCCGGCAAGCAAAGTATCCAGAAATACCGTCCGCTCTGTAAAACAAGCCAGCAGGAATAGGAATATCTTAAAGAAGAAGACAAGGCAACTGGTCTGTGAGAGATGCCAATGTGCAATACAAGGTGCTGGACAACAATCCAATGAAGGTTCTTTGTCTCCCCAGTTTAACGGTCTTGCTAATGAAATAAACTCAAGTAATGTGCACAGCACTCCAGGTAAACCAGGCTTCACCTTGGACAACAAGAAGACTGGCAATACAGGGGGAGCTGGCATCATGAAATCAAAAACGAACTCCAATTCAAAAGAAAGAGGGGAGTTCTCCCAAAGCTCAAAAAGTAGCCAAGGTGATCATAGTAGTAGTACAAGTTTGAGTGACGAGAGCAATGTAAGTGGATCTAGTTATAGCAATAAACCTCACATGTCAAAGGATGTGAGGTGGGAGGCCATCCGTCATGTTCGGTTGCAGCATGGATCCTTGGGCTTGAGACACTTTAACCTTTTGAAAAAGCTTGGTTGTGGAGACATAGGAACTGTATATCTTGCTGAACTCATTAGTACGAACTGTCATTTTGCCATAAAGGTAATGGACAATGAATTCTTGGCTAGAAGGAAGAAGATGCCAAGGGCGCAGACTGAAAGAGAAATACTGAGGATGCTTGATCATCCTTTTCTCCCTACGCTTTATACTCAATTTACATCAGATAATCTGTCATGTTTAGTTACGGAGTATTGCCCAGGCGGAGATCTTCATGTCCTCCGACAGAAGCAAATTGGAAGGTGTTTTTCTGAAGCAGCAACAAGGTTCTATGTTGCGGAAATTCTCCTTGCTTTGGAGTATTTACACATGCTTGGGGTAGTTTATCGGGATTTGAAACCAGAAAACATTCTAGTTCGGGAAGATGGTCATATCATGCTCACAGATTTTGACCTGTCTCTTAGGTGCACTGTCAGTCCAACTCTATTGAAATCACCTTCATGCGATGTTTATCGTGCAAGAGTGTCTGGTCCGTGTACACAATCTAGCTGCGCCGAGCCCTTCTGCATTGAACCCTCCTGTCAAGTCCCATGCTTCAGCCCTAGGTTTCCTGCTGCTGCAAAAGCAAAGAAGTCCAAAAATGACCCTGCATCCCATGTTAGATCATTGCCACAGCTTGTGGCAGAGCCTACTGATGCGCGGTCCAATTCCTTTGTCGGGACCCATGAGTACTTGGCTCCGGAGATCATCAAAGGAGAGGGTCATGGAGCTGCAGTTGATTGGTGGACGTTTGGAATCTTCCTTTACGAGCTTCTGTACGGCAGAACACCATTCAAAGGTATTAACAACGAGGAAACGTTGGCCAATGTGGTGTTGCAGAGCCTAAGGTTCCCAGAGGACCCCCATGTTAGCTTCCAGGCTCGAGATCTCATCAGAGATCTGTTGGTAAAGGAGCCAGAGAATCGGTTGGGATCAGAGAAAGGGGCCGGTGAGATCAAGCAGCATCCGTTCTTTGAGGGCCTGAATTGGGCACTGATACGCTGCGCCACTCCGCCAGAACTGCCCGAGTATTGCGATTTTGAGATCCCGGACATGCCTGCTGCTCAGGAGAATACCAAGTATTTGGAGTTCAAGGCGACGGGAGAGCACCTGGAGTTTGAGTTATTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

883

Amino Acids

96.78

Weight (kDa)

9.13

Isoelectric Point (pI)

48.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 499 - 652 8.2e-36 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 500 - 649 1.6e-19 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 728 - 831 1.7e-21 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 735 - 816 2.7e-08 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 482, 529
AccB7I CCANNNNNTGG 1 cut(s) 305
AccBSI CCGCTC 1 cut(s) 1038
AccII CGCG 1 cut(s) 2179
AccIII TCCGGA 1 cut(s) 2218
AciI CCGC 5 cut(s) 1036, 1743, 1817, 2179, 2531
AclI AACGTT 1 cut(s) 2336
AclWI GGATC 6 cut(s) 443, 1405, 1470, 1483, 2458, 2557
AcoI YGGCCR 1 cut(s) 2340
AcsI RAATTY 4 cut(s) 310, 550, 1598, 1821
AcuI CTGAAG 4 cut(s) 350, 867, 1812, 2059
AcyI GRCGYC 1 cut(s) 483
AfaI GTAC 8 cut(s) 19, 688, 975, 1366, 1561, 2017, 2210, 2297
AfiI CCNNNNNNNGG 6 cut(s) 305, 819, 1834, 2196, 2315, 2506
AflIII ACRYGT 1 cut(s) 1422
AjnI CCWGG 8 cut(s) 91, 298, 946, 1216, 1225, 1738, 2394, 2628
AleI CACNNNNGTG 1 cut(s) 671
Alw21I GWGCWC 5 cut(s) 351, 668, 1209, 1949, 2628
Alw26I GTCTC 5 cut(s) 294, 1164, 1483, 1518, 1941
Alw44I GTGCAC 2 cut(s) 1205, 1945
AlwI GGATC 6 cut(s) 443, 1405, 1470, 1483, 2458, 2557
Ama87I CYCGRG 2 cut(s) 2400, 2543
Aor13HI TCCGGA 1 cut(s) 2218
AoxI GGCC 5 cut(s) 799, 1446, 2340, 2463, 2497
ApaLI GTGCAC 2 cut(s) 1205, 1945
ApoI RAATTY 4 cut(s) 310, 550, 1598, 1821
ArsI GACNNNNNNTTYG 2 cut(s) 818, 850
AseI ATTAAT 1 cut(s) 174
Asp700I GAANNNNTTC 1 cut(s) 1887
AspA2I CCTAGG 1 cut(s) 2081
AspLEI GCGC 5 cut(s) 485, 1633, 2033, 2179, 2525
AspS9I GGNCC 7 cut(s) 800, 2009, 2181, 2199, 2377, 2463, 2497
AsuC2I CCSGG 2 cut(s) 661, 2567
AsuHPI GGTGA 7 cut(s) 119, 288, 1006, 1225, 1361, 1965, 2480
AvaI CYCGRG 2 cut(s) 2400, 2543
AvaII GGWCC 4 cut(s) 2009, 2181, 2199, 2377
AvrII CCTAGG 1 cut(s) 2081
AxyI CCTNAGG 1 cut(s) 2363
BaeGI GKGCMC 3 cut(s) 1209, 1949, 2512
BalI TGGCCA 1 cut(s) 2342
BamHI GGATCC 1 cut(s) 1475
BanI GGYRCC 2 cut(s) 482, 529
BanII GRGCYC 2 cut(s) 351, 2040
BbsI GAAGAC 3 cut(s) 3, 1088, 1256
Bbv12I GWGCWC 5 cut(s) 351, 668, 1209, 1949, 2628
BccI CCATC 2 cut(s) 1457, 1898
BceAI ACGGC 1 cut(s) 2314
BciT130I CCWGG 8 cut(s) 93, 300, 948, 1218, 1227, 1740, 2396, 2630
BciVI GTATCC 2 cut(s) 140, 1030
BclI TGATCA 2 cut(s) 1351, 1663
BcnI CCSGG 2 cut(s) 661, 2567
BcoDI GTCTC 5 cut(s) 294, 1164, 1483, 1518, 1941
BfaI CTAG 9 cut(s) 161, 240, 471, 987, 1401, 1608, 1892, 2025, 2082
BfmI CTRYAG 1 cut(s) 2250
BfoI RGCGCY 1 cut(s) 486
BfuI GTATCC 2 cut(s) 140, 1030
BglII AGATCT 3 cut(s) 1747, 2404, 2416
BlnI CCTAGG 1 cut(s) 2081
BmcAI AGTACT 2 cut(s) 688, 2210
Bme18I GGWCC 4 cut(s) 2009, 2181, 2199, 2377
BmeT110I CYCGRG 2 cut(s) 2400, 2543
BmgT120I GGNCC 7 cut(s) 800, 2009, 2181, 2199, 2377, 2463, 2497
BmrI ACTGGG 2 cut(s) 152, 1159
BmsI GCATC 9 cut(s) 374, 583, 1097, 1284, 1611, 1647, 2138, 2164, 2491
BmuI ACTGGG 2 cut(s) 152, 1159
BpiI GAAGAC 3 cut(s) 3, 1088, 1256
BpmI CTGGAG 1 cut(s) 1200
Bpu10I CCTNAGC 1 cut(s) 2583
BpuEI CTTGAG 3 cut(s) 475, 1180, 1507
BpuMI CCSGG 2 cut(s) 661, 2567
BsaBI GATNNNNATC 1 cut(s) 1989
BsaHI GRCGYC 1 cut(s) 483
BsaWI WCCGGW 1 cut(s) 2218
BsaXI ACNNNNNCTCC 3 cut(s) 2238, 2268, 2624
Bsc4I CCNNNNNNNGG 6 cut(s) 305, 819, 1834, 2196, 2315, 2506
Bse118I RCCGGY 2 cut(s) 319, 2465
Bse1I ACTGG 5 cut(s) 147, 693, 1098, 1165, 1258
Bse21I CCTNAGG 1 cut(s) 2363
Bse3DI GCAATG 2 cut(s) 1393, 2144
Bse8I GATNNNNATC 1 cut(s) 1989
BseAI TCCGGA 1 cut(s) 2218
BseBI CCWGG 8 cut(s) 93, 300, 948, 1218, 1227, 1740, 2396, 2630
BseGI GGATG 8 cut(s) 203, 574, 1438, 1449, 1662, 1666, 2129, 2482
BseJI GATNNNNATC 1 cut(s) 1989
BseLI CCNNNNNNNGG 6 cut(s) 305, 819, 1834, 2196, 2315, 2506
BseMI GCAATG 2 cut(s) 1393, 2144
BseMII CTCAG 2 cut(s) 1643, 2597
BseNI ACTGG 5 cut(s) 147, 693, 1098, 1165, 1258
BseRI GAGGAG 2 cut(s) 478, 615
BseSI GKGCMC 3 cut(s) 1209, 1949, 2512
BseYI CCCAGC 1 cut(s) 841
Bsh1236I CGCG 1 cut(s) 2179
BshFI GGCC 5 cut(s) 801, 1448, 2342, 2465, 2499
BshNI GGYRCC 2 cut(s) 482, 529
BsiHKAI GWGCWC 5 cut(s) 351, 668, 1209, 1949, 2628
BsiHKCI CYCGRG 2 cut(s) 2400, 2543
BsiSI CCGG 6 cut(s) 320, 660, 1007, 2219, 2466, 2567
BslFI GGGAC 2 cut(s) 2051, 2212
BslI CCNNNNNNNGG 6 cut(s) 305, 819, 1834, 2196, 2315, 2506
BsmAI GTCTC 5 cut(s) 294, 1164, 1483, 1518, 1941
BsmFI GGGAC 2 cut(s) 2051, 2212
BsnI GGCC 5 cut(s) 801, 1448, 2342, 2465, 2499
BsoBI CYCGRG 2 cut(s) 2400, 2543
Bsp1286I GDGCHC 7 cut(s) 351, 668, 1209, 1949, 2040, 2512, 2628
Bsp13I TCCGGA 1 cut(s) 2218
Bsp1407I TGTACA 1 cut(s) 2015
BspACI CCGC 5 cut(s) 1036, 1743, 1817, 2179, 2531
BspANI GGCC 5 cut(s) 801, 1448, 2342, 2465, 2499
BspCNI CTCAG 2 cut(s) 1644, 2596
BspEI TCCGGA 1 cut(s) 2218
BspFNI CGCG 1 cut(s) 2179
BspHI TCATGA 1 cut(s) 1278
BspMAI CTGCAG 1 cut(s) 2254
BspPI GGATC 6 cut(s) 443, 1405, 1470, 1483, 2458, 2557
BspT107I GGYRCC 2 cut(s) 482, 529
BsrBI CCGCTC 1 cut(s) 1038
BsrDI GCAATG 2 cut(s) 1393, 2144
BsrFI RCCGGY 2 cut(s) 319, 2465
BsrGI TGTACA 1 cut(s) 2015
BsrI ACTGG 5 cut(s) 147, 693, 1098, 1165, 1258
BssAI RCCGGY 2 cut(s) 319, 2465
BssNI GRCGYC 1 cut(s) 483
BssT1I CCWWGG 7 cut(s) 207, 360, 1236, 1345, 1479, 1625, 2081
Bst2UI CCWGG 8 cut(s) 93, 300, 948, 1218, 1227, 1740, 2396, 2630
BstACI GRCGYC 1 cut(s) 483
BstAUI TGTACA 1 cut(s) 2015
BstC8I GCNNGC 5 cut(s) 336, 1012, 1054, 1273, 2577
BstDEI CTNAG 5 cut(s) 831, 1652, 1940, 2363, 2583
BstF5I GGATG 8 cut(s) 203, 574, 1438, 1449, 1662, 1666, 2129, 2482
BstFNI CGCG 1 cut(s) 2179
BstH2I RGCGCY 1 cut(s) 486
BstHHI GCGC 5 cut(s) 485, 1633, 2033, 2179, 2525
BstMAI GTCTC 5 cut(s) 294, 1164, 1483, 1518, 1941
BstMWI GCNNNNNNNGC 4 cut(s) 571, 1630, 2102, 2539
BstNI CCWGG 8 cut(s) 93, 300, 948, 1218, 1227, 1740, 2396, 2630
BstNSI RCATGY 4 cut(s) 971, 1426, 1852, 2575
BstSFI CTRYAG 1 cut(s) 2250
BstSLI GKGCMC 3 cut(s) 1209, 1949, 2512
BstUI CGCG 1 cut(s) 2179
BstV2I GAAGAC 3 cut(s) 3, 1088, 1256
BstX2I RGATCY 6 cut(s) 1397, 1475, 1747, 2404, 2416, 2562
BstXI CCANNNNNNTGG 1 cut(s) 2602
BstYI RGATCY 6 cut(s) 1397, 1475, 1747, 2404, 2416, 2562
Bsu36I CCTNAGG 1 cut(s) 2363
BsuI GTATCC 2 cut(s) 140, 1030
BsuRI GGCC 5 cut(s) 801, 1448, 2342, 2465, 2499
BtgZI GCGATG 1 cut(s) 1998
BtsCI GGATG 8 cut(s) 203, 574, 1438, 1449, 1662, 1666, 2129, 2482
BtsIMutI CAGTG 3 cut(s) 285, 1947, 2510
Cac8I GCNNGC 5 cut(s) 336, 1012, 1054, 1273, 2577
CciI TCATGA 1 cut(s) 1278
CfoI GCGC 5 cut(s) 485, 1633, 2033, 2179, 2525
Cfr10I RCCGGY 2 cut(s) 319, 2465
Cfr13I GGNCC 7 cut(s) 800, 2009, 2181, 2199, 2377, 2463, 2497
Csp6I GTAC 8 cut(s) 18, 687, 974, 1365, 1560, 2016, 2209, 2296
CspCI CAANNNNNGTGG 4 cut(s) 261, 296, 1375, 1410
CviQI GTAC 8 cut(s) 18, 687, 974, 1365, 1560, 2016, 2209, 2296
DdeI CTNAG 5 cut(s) 831, 1652, 1940, 2363, 2583
DinI GGCGCC 1 cut(s) 484
DraI TTTAAA 1 cut(s) 249
EaeI YGGCCR 1 cut(s) 2340
EciI GGCGGA 2 cut(s) 1758, 2520
Ecl136II GAGCTC 1 cut(s) 349
Eco130I CCWWGG 7 cut(s) 207, 360, 1236, 1345, 1479, 1625, 2081
Eco24I GRGCYC 2 cut(s) 351, 2040
Eco47I GGWCC 4 cut(s) 2009, 2181, 2199, 2377
Eco53kI GAGCTC 1 cut(s) 349
Eco57I CTGAAG 4 cut(s) 350, 867, 1812, 2059
Eco81I CCTNAGG 1 cut(s) 2363
Eco88I CYCGRG 2 cut(s) 2400, 2543
EcoICRI GAGCTC 1 cut(s) 349
EcoO109I RGGNCCY 3 cut(s) 2199, 2377, 2497
EcoRI GAATTC 2 cut(s) 310, 1598
EcoRII CCWGG 8 cut(s) 91, 298, 946, 1216, 1225, 1738, 2394, 2628
EcoT14I CCWWGG 7 cut(s) 207, 360, 1236, 1345, 1479, 1625, 2081
EcoT38I GRGCYC 2 cut(s) 351, 2040
EgeI GGCGCC 1 cut(s) 484
EheI GGCGCC 1 cut(s) 484
ErhI CCWWGG 7 cut(s) 207, 360, 1236, 1345, 1479, 1625, 2081
FalI AAGNNNNNCTT 2 cut(s) 843, 875
FaqI GGGAC 2 cut(s) 2051, 2212
FbaI TGATCA 2 cut(s) 1351, 1663
FokI GGATG 8 cut(s) 190, 561, 1436, 1445, 1653, 1669, 2116, 2469
FriOI GRGCYC 2 cut(s) 351, 2040
FspBI CTAG 9 cut(s) 161, 240, 471, 987, 1401, 1608, 1892, 2025, 2082
GlaI GCGC 5 cut(s) 484, 1632, 2032, 2178, 2524
GsaI CCCAGC 1 cut(s) 845
GsuI CTGGAG 1 cut(s) 1200
HaeII RGCGCY 1 cut(s) 486
HaeIII GGCC 5 cut(s) 801, 1448, 2342, 2465, 2499
HapII CCGG 6 cut(s) 320, 660, 1007, 2219, 2466, 2567
HhaI GCGC 5 cut(s) 485, 1633, 2033, 2179, 2525
Hin1I GRCGYC 1 cut(s) 483
Hin6I GCGC 5 cut(s) 483, 1631, 2031, 2177, 2523
HinP1I GCGC 5 cut(s) 483, 1631, 2031, 2177, 2523
HindIII AAGCTT 2 cut(s) 122, 1511
HinfI GANTC 6 cut(s) 88, 155, 325, 425, 2274, 2440
HpaII CCGG 6 cut(s) 320, 660, 1007, 2219, 2466, 2567
HphI GGTGA 7 cut(s) 119, 288, 1006, 1225, 1361, 1965, 2480
Hpy166II GTNNAC 7 cut(s) 614, 1207, 1223, 1947, 2016, 2018, 2265
Hpy8I GTNNAC 7 cut(s) 614, 1207, 1223, 1947, 2016, 2018, 2265
Hpy99I CGWCG 1 cut(s) 2620
HpyAV CCTTC 9 cut(s) 420, 742, 813, 987, 1142, 1605, 1773, 1986, 2050
HpyCH4IV ACGT 3 cut(s) 443, 2267, 2336
HpyF10VI GCNNNNNNNGC 4 cut(s) 571, 1630, 2102, 2539
HpyF3I CTNAG 5 cut(s) 831, 1652, 1940, 2363, 2583
HpySE526I ACGT 3 cut(s) 443, 2267, 2336
Hsp92I GRCGYC 1 cut(s) 483
HspAI GCGC 5 cut(s) 483, 1631, 2031, 2177, 2523
KasI GGCGCC 1 cut(s) 482
KflI GGGWCCC 1 cut(s) 2199
Kpn2I TCCGGA 1 cut(s) 2218
Ksp22I TGATCA 2 cut(s) 1351, 1663
LmnI GCTCC 8 cut(s) 562, 663, 718, 838, 1268, 2221, 2246, 2431
LweI GCATC 9 cut(s) 374, 583, 1097, 1284, 1611, 1647, 2138, 2164, 2491
MaeI CTAG 9 cut(s) 161, 240, 471, 987, 1401, 1608, 1892, 2025, 2082
MaeII ACGT 3 cut(s) 443, 2267, 2336
MaeIII GTNAC 4 cut(s) 473, 816, 1376, 1723
MbiI CCGCTC 1 cut(s) 1038
MflI RGATCY 6 cut(s) 1397, 1475, 1747, 2404, 2416, 2562
MhlI GDGCHC 7 cut(s) 351, 668, 1209, 1949, 2040, 2512, 2628
MlsI TGGCCA 1 cut(s) 2342
MluNI TGGCCA 1 cut(s) 2342
Mly113I GGCGCC 1 cut(s) 483
MlyI GAGTC 1 cut(s) 319
MmeI TCCRAC 4 cut(s) 816, 831, 1786, 1982
Mox20I TGGCCA 1 cut(s) 2342
MroI TCCGGA 1 cut(s) 2218
MroXI GAANNNNTTC 1 cut(s) 1887
MscI TGGCCA 1 cut(s) 2342
MslI CAYNNNNRTG 2 cut(s) 671, 1978
Msp20I TGGCCA 1 cut(s) 2342
MspI CCGG 6 cut(s) 320, 660, 1007, 2219, 2466, 2567
MvaI CCWGG 8 cut(s) 93, 300, 948, 1218, 1227, 1740, 2396, 2630
MvnI CGCG 1 cut(s) 2179
MwoI GCNNNNNNNGC 4 cut(s) 571, 1630, 2102, 2539
NarI GGCGCC 1 cut(s) 483
NciI CCSGG 2 cut(s) 661, 2567
NmeAIII GCCGAG 1 cut(s) 2059
NmuCI GTSAC 2 cut(s) 473, 1376
NspI RCATGY 4 cut(s) 971, 1426, 1852, 2575
OliI CACNNNNGTG 1 cut(s) 671
PaeR7I CTCGAG 1 cut(s) 2400
PagI TCATGA 1 cut(s) 1278
PciI ACATGT 1 cut(s) 1422
PdmI GAANNNNTTC 1 cut(s) 1887
PfeI GAWTC 5 cut(s) 88, 155, 425, 2274, 2440
PflMI CCANNNNNTGG 1 cut(s) 305
PfoI TCCNGGA 3 cut(s) 91, 659, 2565
PleI GAGTC 1 cut(s) 319
PluTI GGCGCC 1 cut(s) 486
PpsI GAGTC 1 cut(s) 319
PpuMI RGGWCCY 2 cut(s) 2199, 2377
PscI ACATGT 1 cut(s) 1422
PshBI ATTAAT 1 cut(s) 174
Psp124BI GAGCTC 1 cut(s) 351
Psp1406I AACGTT 1 cut(s) 2336
Psp5II RGGWCCY 2 cut(s) 2199, 2377
Psp6I CCWGG 8 cut(s) 91, 298, 946, 1216, 1225, 1738, 2394, 2628
PspFI CCCAGC 1 cut(s) 841
PspGI CCWGG 8 cut(s) 91, 298, 946, 1216, 1225, 1738, 2394, 2628
PspPI GGNCC 7 cut(s) 800, 2009, 2181, 2199, 2377, 2463, 2497
PspPPI RGGWCCY 2 cut(s) 2199, 2377
PsrI GAACNNNNNNTAC 2 cut(s) 2591, 2623
PstI CTGCAG 1 cut(s) 2254
PsuI RGATCY 6 cut(s) 1397, 1475, 1747, 2404, 2416, 2562
RsaI GTAC 8 cut(s) 19, 688, 975, 1366, 1561, 2017, 2210, 2297
RsaNI GTAC 8 cut(s) 18, 687, 974, 1365, 1560, 2016, 2209, 2296
RseI CAYNNNNRTG 2 cut(s) 671, 1978
SacI GAGCTC 1 cut(s) 351
Sau96I GGNCC 7 cut(s) 800, 2009, 2181, 2199, 2377, 2463, 2497
ScaI AGTACT 2 cut(s) 688, 2210
SchI GAGTC 1 cut(s) 319
SduI GDGCHC 7 cut(s) 351, 668, 1209, 1949, 2040, 2512, 2628
SfaNI GCATC 9 cut(s) 374, 583, 1097, 1284, 1611, 1647, 2138, 2164, 2491
SfcI CTRYAG 1 cut(s) 2250
SfoI GGCGCC 1 cut(s) 484
Sfr274I CTCGAG 1 cut(s) 2400
SinI GGWCC 4 cut(s) 2009, 2181, 2199, 2377
SlaI CTCGAG 1 cut(s) 2400
SmiMI CAYNNNNRTG 2 cut(s) 671, 1978
SmlI CTYRAG 4 cut(s) 490, 1195, 1486, 2400
SmoI CTYRAG 4 cut(s) 490, 1195, 1486, 2400
SsiI CCGC 5 cut(s) 1036, 1743, 1817, 2179, 2531
SspDI GGCGCC 1 cut(s) 482
SspI AATATT 1 cut(s) 65
SspMI CTAG 9 cut(s) 161, 240, 471, 987, 1401, 1608, 1892, 2025, 2082
SstI GAGCTC 1 cut(s) 351
StyI CCWWGG 7 cut(s) 207, 360, 1236, 1345, 1479, 1625, 2081
TaiI ACGT 3 cut(s) 446, 2270, 2339
TaqI TCGA 3 cut(s) 82, 628, 2401
TatI WGTACW 4 cut(s) 686, 1364, 2015, 2208
TfiI GAWTC 5 cut(s) 88, 155, 425, 2274, 2440
TscAI CASTG 3 cut(s) 285, 1954, 2517
TseFI GTSAC 2 cut(s) 473, 1376
Tsp45I GTSAC 2 cut(s) 473, 1376
TspGWI ACGGA 6 cut(s) 87, 835, 1442, 1742, 2001, 2475
TspRI CASTG 3 cut(s) 285, 1954, 2517
Van91I CCANNNNNTGG 1 cut(s) 305
VneI GTGCAC 2 cut(s) 1205, 1945
VpaK11BI GGWCC 4 cut(s) 2009, 2181, 2199, 2377
VspI ATTAAT 1 cut(s) 174
XapI RAATTY 4 cut(s) 310, 550, 1598, 1821
XbaI TCTAGA 1 cut(s) 160
XceI RCATGY 4 cut(s) 971, 1426, 1852, 2575
XcmI CCANNNNNNNNNTGG 1 cut(s) 1585
XhoI CTCGAG 1 cut(s) 2400
XmaJI CCTAGG 1 cut(s) 2081
XmnI GAANNNNTTC 1 cut(s) 1887
XspI CTAG 9 cut(s) 161, 240, 471, 987, 1401, 1608, 1892, 2025, 2082
ZrmI AGTACT 2 cut(s) 688, 2210
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.