FvH4_6g19290

1-aminocyclopropane-1-carboxylate oxidase homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0010514
Physical Location & Seq
Forward (+)
12799956 .. 12800684
729 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g19290.t1

Sequence Viewer

Length: 627 bp
ATGGCACCAGAGGAGTTGAAGGTGGAGGAGGACATCCCAGATATATGTCGTAAGGAGATTATTGCATGGGATTTTCATGCATCCAAAGTAGCCGAGGCGTTATTTGAGCTGTTGGCCGAGGGGCTTGGTTTGGAAGCTGGGAAGTTCAAGGAGTTGGGTTTTATGAAAGCTAGGTTATTCTTGGGGCACTGCTATCCCTACTGTCCGCAACCCGATCTGACAGTGGGGATCAAATCTCATATTGATGCAGGTTTGACGGTGTTGCTGCAGAACCAGGTCGGAGGGTTGCAAGTGAAGCATGGGAGTGAGTGGGTGGATGTCAAACCATTGCCTGGGGCATTGACTGTTAATATTGGCGACTTCATACAGATCATATCGAATGGGGAGTATCAAAGCGTAGAGCATCGAGTATTGGCAAACTCATCAAAAGAACCAAGAATCTCAGTCTTGATTTTTAATAACGTGGGGGGTTGGAAAGAGTCGGATGAATTTGGGCCATTTCCAGAATTACTATCTGCAGAGAAAGCAGCCCTTTATCGAGGATTCACTGTGCCAGAGTTCCTTCAGAGCTTTTACAGTAAAGGGTTAGATGGCAAAAACATAGTAGAAAGAGTTACACTACACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

209

Amino Acids

23.07

Weight (kDa)

5.17

Isoelectric Point (pI)

33.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2OG-FeII_Oxy PF03171 63 - 152 7.4e-28 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 239
AccB1I GGYRCC 1 cut(s) 4
AccB7I CCANNNNNTGG 1 cut(s) 332
AciI CCGC 1 cut(s) 206
AclWI GGATC 1 cut(s) 236
AcoI YGGCCR 1 cut(s) 114
AcsI RAATTY 1 cut(s) 488
AcuI CTGAAG 1 cut(s) 548
AfiI CCNNNNNNNGG 1 cut(s) 332
AgsI TTSAA 2 cut(s) 19, 148
AjnI CCWGG 2 cut(s) 273, 331
AluBI AGCT 4 cut(s) 109, 137, 170, 570
AluI AGCT 4 cut(s) 109, 137, 170, 570
AlwI GGATC 1 cut(s) 236
AoxI GGCC 2 cut(s) 114, 494
ApeKI GCWGC 2 cut(s) 265, 527
ApoI RAATTY 1 cut(s) 488
AspS9I GGNCC 1 cut(s) 494
BaeGI GKGCMC 1 cut(s) 189
BanI GGYRCC 1 cut(s) 4
BbvI GCAGC 2 cut(s) 252, 539
BccI CCATC 1 cut(s) 584
BciT130I CCWGG 2 cut(s) 275, 333
BfaI CTAG 2 cut(s) 171, 625
BfmI CTRYAG 2 cut(s) 266, 516
BfuAI ACCTGC 1 cut(s) 239
BisI GCNGC 2 cut(s) 266, 528
BlsI GCNGC 2 cut(s) 267, 529
Bme1390I CCNGG 2 cut(s) 275, 333
BmgT120I GGNCC 1 cut(s) 494
BmiI GGNNCC 1 cut(s) 6
BmrFI CCNGG 2 cut(s) 275, 333
BmsI GCATC 3 cut(s) 89, 235, 412
BsaJI CCNNGG 3 cut(s) 93, 117, 332
Bsc4I CCNNNNNNNGG 1 cut(s) 332
Bse3DI GCAATG 1 cut(s) 326
BseBI CCWGG 2 cut(s) 275, 333
BseDI CCNNGG 3 cut(s) 93, 117, 332
BseGI GGATG 4 cut(s) 33, 80, 322, 490
BseLI CCNNNNNNNGG 1 cut(s) 332
BseMI GCAATG 1 cut(s) 326
BseMII CTCAG 1 cut(s) 456
BseRI GAGGAG 2 cut(s) 26, 41
BseSI GKGCMC 1 cut(s) 189
BseXI GCAGC 2 cut(s) 252, 539
BseYI CCCAGC 1 cut(s) 137
BshFI GGCC 2 cut(s) 116, 496
BshNI GGYRCC 1 cut(s) 4
BslI CCNNNNNNNGG 1 cut(s) 332
BsnI GGCC 2 cut(s) 116, 496
Bsp1286I GDGCHC 1 cut(s) 189
Bsp143I GATC 3 cut(s) 214, 228, 369
BspACI CCGC 1 cut(s) 206
BspANI GGCC 2 cut(s) 116, 496
BspCNI CTCAG 1 cut(s) 455
BspLI GGNNCC 1 cut(s) 6
BspMAI CTGCAG 2 cut(s) 270, 520
BspMI ACCTGC 1 cut(s) 239
BspPI GGATC 1 cut(s) 236
BspT107I GGYRCC 1 cut(s) 4
BsrDI GCAATG 1 cut(s) 326
BssECI CCNNGG 3 cut(s) 93, 117, 332
BssMI GATC 3 cut(s) 214, 228, 369
Bst2UI CCWGG 2 cut(s) 275, 333
Bst4CI ACNGT 6 cut(s) 203, 223, 259, 346, 550, 578
BstDEI CTNAG 1 cut(s) 442
BstF5I GGATG 4 cut(s) 33, 80, 322, 490
BstKTI GATC 3 cut(s) 217, 231, 372
BstMBI GATC 3 cut(s) 214, 228, 369
BstMWI GCNNNNNNNGC 2 cut(s) 295, 524
BstNI CCWGG 2 cut(s) 275, 333
BstSCI CCNGG 2 cut(s) 273, 331
BstSFI CTRYAG 2 cut(s) 266, 516
BstSLI GKGCMC 1 cut(s) 189
BstV1I GCAGC 2 cut(s) 252, 539
BsuRI GGCC 2 cut(s) 116, 496
BtsCI GGATG 4 cut(s) 33, 80, 322, 490
BtsI GCAGTG 1 cut(s) 187
BtsIMutI CAGTG 3 cut(s) 187, 228, 546
BveI ACCTGC 1 cut(s) 239
Cfr13I GGNCC 1 cut(s) 494
CsiI ACCWGGT 1 cut(s) 273
CviAII CATG 3 cut(s) 66, 77, 299
CviJI RGCY 9 cut(s) 92, 109, 116, 124, 137, 170, 496, 530, 570
CviKI_1 RGCY 9 cut(s) 92, 109, 116, 124, 137, 170, 496, 530, 570
DdeI CTNAG 1 cut(s) 442
DpnI GATC 3 cut(s) 216, 230, 371
DpnII GATC 3 cut(s) 214, 228, 369
EaeI YGGCCR 1 cut(s) 114
Eco57I CTGAAG 1 cut(s) 548
EcoRII CCWGG 2 cut(s) 273, 331
EcoT22I ATGCAT 1 cut(s) 82
FaeI CATG 3 cut(s) 69, 80, 302
FalI AAGNNNNNCTT 2 cut(s) 516, 548
FatI CATG 3 cut(s) 65, 76, 298
Fnu4HI GCNGC 2 cut(s) 266, 528
FokI GGATG 4 cut(s) 20, 67, 329, 497
Fsp4HI GCNGC 2 cut(s) 266, 528
FspBI CTAG 2 cut(s) 171, 625
GluI GCNGC 2 cut(s) 266, 528
GsaI CCCAGC 1 cut(s) 141
HaeIII GGCC 2 cut(s) 116, 496
Hin1II CATG 3 cut(s) 69, 80, 302
HinfI GANTC 3 cut(s) 438, 479, 543
Hpy188I TCNGA 4 cut(s) 219, 281, 484, 567
Hpy188III TCNNGA 2 cut(s) 448, 503
HpyAV CCTTC 2 cut(s) 13, 572
HpyCH4III ACNGT 6 cut(s) 203, 223, 259, 346, 550, 578
HpyCH4IV ACGT 1 cut(s) 462
HpyCH4V TGCA 6 cut(s) 65, 80, 248, 268, 289, 518
HpyF10VI GCNNNNNNNGC 2 cut(s) 295, 524
HpyF3I CTNAG 1 cut(s) 442
HpySE526I ACGT 1 cut(s) 462
Hsp92II CATG 3 cut(s) 69, 80, 302
Kzo9I GATC 3 cut(s) 214, 228, 369
Lsp1109I GCAGC 2 cut(s) 252, 539
LweI GCATC 3 cut(s) 89, 235, 412
MabI ACCWGGT 1 cut(s) 273
MaeI CTAG 2 cut(s) 171, 625
MaeII ACGT 1 cut(s) 462
MaeIII GTNAC 1 cut(s) 613
MalI GATC 3 cut(s) 216, 230, 371
MboI GATC 3 cut(s) 214, 228, 369
MhlI GDGCHC 1 cut(s) 189
MluCI AATT 2 cut(s) 488, 506
MlyI GAGTC 1 cut(s) 488
MmeI TCCRAC 3 cut(s) 259, 452, 462
MnlI CCTC 7 cut(s) 4, 19, 22, 88, 112, 275, 533
Mph1103I ATGCAT 1 cut(s) 82
MseI TTAA 2 cut(s) 348, 456
MslI CAYNNNNRTG 2 cut(s) 243, 303
MspR9I CCNGG 2 cut(s) 275, 333
MvaI CCWGG 2 cut(s) 275, 333
MwoI GCNNNNNNNGC 2 cut(s) 295, 524
NdeII GATC 3 cut(s) 214, 228, 369
NlaIII CATG 3 cut(s) 69, 80, 302
NlaIV GGNNCC 1 cut(s) 6
NmeAIII GCCGAG 2 cut(s) 118, 142
NsiI ATGCAT 1 cut(s) 82
PfeI GAWTC 2 cut(s) 438, 543
PflMI CCANNNNNTGG 1 cut(s) 332
PkrI GCNGC 2 cut(s) 267, 529
PleI GAGTC 1 cut(s) 487
PpsI GAGTC 1 cut(s) 487
Psp6I CCWGG 2 cut(s) 273, 331
PspFI CCCAGC 1 cut(s) 137
PspGI CCWGG 2 cut(s) 273, 331
PspN4I GGNNCC 1 cut(s) 6
PspPI GGNCC 1 cut(s) 494
PstI CTGCAG 2 cut(s) 270, 520
RseI CAYNNNNRTG 2 cut(s) 243, 303
SaqAI TTAA 2 cut(s) 348, 456
SatI GCNGC 2 cut(s) 266, 528
Sau3AI GATC 3 cut(s) 214, 228, 369
Sau96I GGNCC 1 cut(s) 494
SchI GAGTC 1 cut(s) 488
ScrFI CCNGG 2 cut(s) 275, 333
SduI GDGCHC 1 cut(s) 189
SetI ASST 9 cut(s) 24, 111, 139, 172, 176, 253, 279, 465, 572
SexAI ACCWGGT 1 cut(s) 273
SfaNI GCATC 3 cut(s) 89, 235, 412
SfcI CTRYAG 2 cut(s) 266, 516
SmiMI CAYNNNNRTG 2 cut(s) 243, 303
Sse9I AATT 2 cut(s) 488, 506
SsiI CCGC 1 cut(s) 206
SspI AATATT 1 cut(s) 352
SspMI CTAG 2 cut(s) 171, 625
StyD4I CCNGG 2 cut(s) 273, 331
TaaI ACNGT 6 cut(s) 203, 223, 259, 346, 550, 578
TaiI ACGT 1 cut(s) 465
TaqI TCGA 3 cut(s) 377, 406, 538
TasI AATT 2 cut(s) 488, 506
TfiI GAWTC 2 cut(s) 438, 543
Tru1I TTAA 2 cut(s) 348, 456
Tru9I TTAA 2 cut(s) 348, 456
TscAI CASTG 3 cut(s) 194, 228, 553
TseI GCWGC 2 cut(s) 265, 527
TspDTI ATGAA 4 cut(s) 65, 179, 352, 501
TspRI CASTG 3 cut(s) 194, 228, 553
Van91I CCANNNNNTGG 1 cut(s) 332
XapI RAATTY 1 cut(s) 488
XspI CTAG 2 cut(s) 171, 625
Zsp2I ATGCAT 1 cut(s) 82
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.