Rroxscaffold_6G00407210

1-aminocyclopropane-1-carboxylate oxidase homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0010514
Physical Location & Seq
Reverse (-)
29843409 .. 29844964
1556 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00407210.1

Sequence Viewer

Length: 609 bp
ATGAAGGTGGAGGATGACATCCCAGAGGTTTGCCGTAAGGAGGTGATTGCATGGGATTTTCATGCAGCTAAAGTAGCCGCGGCATTATTTGAGTTGTTGGCTGAAGGTCTTGGTTTGGAACCTAGGAAGTTCAAGGAGTTGGGTTTTATGAAAGCTAGATTATTTTTGGGGCACTGTTATCCCTACTGTCCGCAACCAGATCTGACGGTGGGAATCAAATCTCATACTGATGCCGGTTTGACAGTGTTGCTGCAGAACCAGGTCGGAGGGTTGCAAGTGAAGCAAGGGAGTGAGTGGGTGGATGTTAAGCCCTTGCCTGGGGCATTGACTGTTAATATTGGTGACTTCATACAGATCATATCAAATGGGGATTATCAAAGCGTGGAGCATCGAGTATTGGCAAACTCATCGAAGGAACCAAGAATCTCGGTATTGATTTTTAATAACTTGGGGGTTGGAAAGAGTCGGATGAATTTGGGCCACTTCCGAATTATTATCAGCAGAGAAACCAGCCCTTTATCGAGGATTCACTATGCCGAGTTTGTTCAAAGCTTTTATAGCAAAGGATTAGATGGCAAAAACATAGTAGAAAGGGTTACACTACACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

202

Amino Acids

22.51

Weight (kDa)

7.76

Isoelectric Point (pI)

30.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2OG-FeII_Oxy PF03171 58 - 147 2.6e-28 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 80
AciI CCGC 3 cut(s) 78, 80, 191
AcsI RAATTY 1 cut(s) 472
AcuI CTGAAG 1 cut(s) 123
AfiI CCNNNNNNNGG 3 cut(s) 40, 317, 318
AgsI TTSAA 2 cut(s) 133, 548
AjnI CCWGG 2 cut(s) 258, 316
AluBI AGCT 3 cut(s) 68, 155, 552
AluI AGCT 3 cut(s) 68, 155, 552
AoxI GGCC 1 cut(s) 478
ApeKI GCWGC 2 cut(s) 65, 250
ApoI RAATTY 1 cut(s) 472
AspA2I CCTAGG 1 cut(s) 122
AspS9I GGNCC 1 cut(s) 478
AsuHPI GGTGA 2 cut(s) 55, 353
AvrII CCTAGG 1 cut(s) 122
BaeGI GKGCMC 1 cut(s) 174
BbvI GCAGC 2 cut(s) 77, 237
BccI CCATC 1 cut(s) 566
BceAI ACGGC 1 cut(s) 18
BcgI CGANNNNNNTGC 2 cut(s) 390, 424
BciT130I CCWGG 2 cut(s) 260, 318
BfaI CTAG 3 cut(s) 123, 156, 607
BfmI CTRYAG 1 cut(s) 251
BglII AGATCT 1 cut(s) 199
BisI GCNGC 4 cut(s) 66, 78, 81, 251
BlnI CCTAGG 1 cut(s) 122
BlsI GCNGC 4 cut(s) 67, 79, 82, 252
Bme1390I CCNGG 2 cut(s) 260, 318
BmgT120I GGNCC 1 cut(s) 478
BmiI GGNNCC 2 cut(s) 120, 417
BmrFI CCNGG 2 cut(s) 260, 318
BmsI GCATC 2 cut(s) 220, 397
BsaJI CCNNGG 3 cut(s) 78, 122, 317
Bsc4I CCNNNNNNNGG 3 cut(s) 40, 317, 318
Bse118I RCCGGY 1 cut(s) 233
BseBI CCWGG 2 cut(s) 260, 318
BseDI CCNNGG 3 cut(s) 78, 122, 317
BseGI GGATG 4 cut(s) 18, 19, 307, 474
BseLI CCNNNNNNNGG 3 cut(s) 40, 317, 318
BseSI GKGCMC 1 cut(s) 174
BseXI GCAGC 2 cut(s) 77, 237
Bsh1236I CGCG 1 cut(s) 80
BshFI GGCC 1 cut(s) 480
BsiSI CCGG 1 cut(s) 234
BslI CCNNNNNNNGG 3 cut(s) 40, 317, 318
BsnI GGCC 1 cut(s) 480
Bsp1286I GDGCHC 1 cut(s) 174
Bsp143I GATC 2 cut(s) 199, 354
BspACI CCGC 3 cut(s) 78, 80, 191
BspANI GGCC 1 cut(s) 480
BspFNI CGCG 1 cut(s) 80
BspLI GGNNCC 2 cut(s) 120, 417
BspMAI CTGCAG 1 cut(s) 255
BsrFI RCCGGY 1 cut(s) 233
BssAI RCCGGY 1 cut(s) 233
BssECI CCNNGG 3 cut(s) 78, 122, 317
BssMI GATC 2 cut(s) 199, 354
BssT1I CCWWGG 1 cut(s) 122
Bst2UI CCWGG 2 cut(s) 260, 318
Bst4CI ACNGT 5 cut(s) 176, 188, 208, 244, 331
BstDSI CCRYGG 1 cut(s) 78
BstF5I GGATG 4 cut(s) 18, 19, 307, 474
BstFNI CGCG 1 cut(s) 80
BstKTI GATC 2 cut(s) 202, 357
BstMBI GATC 2 cut(s) 199, 354
BstMWI GCNNNNNNNGC 3 cut(s) 74, 280, 558
BstNI CCWGG 2 cut(s) 260, 318
BstSCI CCNGG 2 cut(s) 258, 316
BstSFI CTRYAG 1 cut(s) 251
BstSLI GKGCMC 1 cut(s) 174
BstUI CGCG 1 cut(s) 80
BstV1I GCAGC 2 cut(s) 77, 237
BstX2I RGATCY 1 cut(s) 199
BstYI RGATCY 1 cut(s) 199
BsuRI GGCC 1 cut(s) 480
BtgI CCRYGG 1 cut(s) 78
BtsCI GGATG 4 cut(s) 18, 19, 307, 474
BtsIMutI CAGTG 2 cut(s) 172, 249
Cfr10I RCCGGY 1 cut(s) 233
Cfr13I GGNCC 1 cut(s) 478
Cfr42I CCGCGG 1 cut(s) 81
CsiI ACCWGGT 1 cut(s) 258
CviAII CATG 2 cut(s) 51, 62
CviJI RGCY 8 cut(s) 68, 77, 101, 155, 310, 480, 513, 552
CviKI_1 RGCY 8 cut(s) 68, 77, 101, 155, 310, 480, 513, 552
DpnI GATC 2 cut(s) 201, 356
DpnII GATC 2 cut(s) 199, 354
Eco130I CCWWGG 1 cut(s) 122
Eco57I CTGAAG 1 cut(s) 123
EcoRII CCWGG 2 cut(s) 258, 316
EcoT14I CCWWGG 1 cut(s) 122
ErhI CCWWGG 1 cut(s) 122
FaeI CATG 2 cut(s) 54, 65
FaiI YATR 9 cut(s) 52, 63, 149, 225, 350, 359, 534, 558, 584
FatI CATG 2 cut(s) 50, 61
Fnu4HI GCNGC 4 cut(s) 66, 78, 81, 251
FokI GGATG 4 cut(s) 5, 26, 314, 481
Fsp4HI GCNGC 4 cut(s) 66, 78, 81, 251
FspBI CTAG 3 cut(s) 123, 156, 607
GluI GCNGC 4 cut(s) 66, 78, 81, 251
HaeIII GGCC 1 cut(s) 480
HapII CCGG 1 cut(s) 234
Hin1II CATG 2 cut(s) 54, 65
HindIII AAGCTT 1 cut(s) 550
HinfI GANTC 4 cut(s) 213, 423, 463, 526
HpaII CCGG 1 cut(s) 234
HphI GGTGA 2 cut(s) 55, 353
Hpy188I TCNGA 4 cut(s) 204, 266, 468, 488
HpyAV CCTTC 2 cut(s) 98, 406
HpyCH4III ACNGT 5 cut(s) 176, 188, 208, 244, 331
HpyCH4V TGCA 4 cut(s) 50, 65, 253, 274
HpyF10VI GCNNNNNNNGC 3 cut(s) 74, 280, 558
Hsp92II CATG 2 cut(s) 54, 65
KspI CCGCGG 1 cut(s) 81
Kzo9I GATC 2 cut(s) 199, 354
LmnI GCTCC 1 cut(s) 385
LpnPI CCDG 8 cut(s) 36, 210, 245, 247, 272, 303, 330, 523
Lsp1109I GCAGC 2 cut(s) 77, 237
LweI GCATC 2 cut(s) 220, 397
MabI ACCWGGT 1 cut(s) 258
MaeI CTAG 3 cut(s) 123, 156, 607
MaeIII GTNAC 2 cut(s) 341, 595
MalI GATC 2 cut(s) 201, 356
MboI GATC 2 cut(s) 199, 354
MflI RGATCY 1 cut(s) 199
MhlI GDGCHC 1 cut(s) 174
MluCI AATT 2 cut(s) 472, 489
MlyI GAGTC 1 cut(s) 472
MmeI TCCRAC 3 cut(s) 244, 436, 446
MnlI CCTC 5 cut(s) 4, 19, 34, 260, 516
MseI TTAA 3 cut(s) 306, 333, 441
MslI CAYNNNNRTG 1 cut(s) 228
MspA1I CMGCKG 1 cut(s) 80
MspI CCGG 1 cut(s) 234
MspR9I CCNGG 2 cut(s) 260, 318
MvaI CCWGG 2 cut(s) 260, 318
MvnI CGCG 1 cut(s) 80
MwoI GCNNNNNNNGC 3 cut(s) 74, 280, 558
NdeII GATC 2 cut(s) 199, 354
NlaIII CATG 2 cut(s) 54, 65
NlaIV GGNNCC 2 cut(s) 120, 417
NmeAIII GCCGAG 1 cut(s) 562
NmuCI GTSAC 1 cut(s) 341
PfeI GAWTC 3 cut(s) 213, 423, 526
PkrI GCNGC 4 cut(s) 67, 79, 82, 252
PleI GAGTC 1 cut(s) 471
PpsI GAGTC 1 cut(s) 471
Psp6I CCWGG 2 cut(s) 258, 316
PspGI CCWGG 2 cut(s) 258, 316
PspN4I GGNNCC 2 cut(s) 120, 417
PspPI GGNCC 1 cut(s) 478
PstI CTGCAG 1 cut(s) 255
PsuI RGATCY 1 cut(s) 199
RseI CAYNNNNRTG 1 cut(s) 228
SacII CCGCGG 1 cut(s) 81
SaqAI TTAA 3 cut(s) 306, 333, 441
SatI GCNGC 4 cut(s) 66, 78, 81, 251
Sau3AI GATC 2 cut(s) 199, 354
Sau96I GGNCC 1 cut(s) 478
SchI GAGTC 1 cut(s) 472
ScrFI CCNGG 2 cut(s) 260, 318
SduI GDGCHC 1 cut(s) 174
SetI ASST 9 cut(s) 9, 30, 45, 70, 109, 124, 157, 264, 554
SexAI ACCWGGT 1 cut(s) 258
SfaNI GCATC 2 cut(s) 220, 397
SfcI CTRYAG 1 cut(s) 251
Sfr303I CCGCGG 1 cut(s) 81
SgrBI CCGCGG 1 cut(s) 81
SmiMI CAYNNNNRTG 1 cut(s) 228
Sse9I AATT 2 cut(s) 472, 489
SsiI CCGC 3 cut(s) 78, 80, 191
SspI AATATT 1 cut(s) 337
SspMI CTAG 3 cut(s) 123, 156, 607
StyD4I CCNGG 2 cut(s) 258, 316
StyI CCWWGG 1 cut(s) 122
TaaI ACNGT 5 cut(s) 176, 188, 208, 244, 331
TaqI TCGA 3 cut(s) 391, 410, 521
TasI AATT 2 cut(s) 472, 489
TauI GCSGC 2 cut(s) 80, 83
TfiI GAWTC 3 cut(s) 213, 423, 526
Tru1I TTAA 3 cut(s) 306, 333, 441
Tru9I TTAA 3 cut(s) 306, 333, 441
TscAI CASTG 2 cut(s) 179, 249
TseFI GTSAC 1 cut(s) 341
TseI GCWGC 2 cut(s) 65, 250
Tsp45I GTSAC 1 cut(s) 341
TspDTI ATGAA 5 cut(s) 17, 50, 164, 337, 485
TspRI CASTG 2 cut(s) 179, 249
XapI RAATTY 1 cut(s) 472
XmaJI CCTAGG 1 cut(s) 122
XspI CTAG 3 cut(s) 123, 156, 607
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.