FvH4_6g19350

GMC oxidoreductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0003277
Physical Location & Seq
Reverse (-)
12872592 .. 12879363
6772 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g19350.t4

Sequence Viewer

Length: 3558 bp
ATGGTTGAGAAACAGGCAGCTGTAGAAGAAAGTTTTGGTGATGGACAAGAAGATAGTTATGATGCTGTTGTTGTGGGGTCTGGATATGGTGGTTCTGTTGCAGCTTGCCGCTTGTCTATGGCAGGTGTAAGAGTATGTCTGATTGAGAAGGGCCGGAAATGGGAGTCTCAGGATTTTCCAACTGATGTCTCCAAAATCTTGTCGGCTTTCAGAATGGAGAACCAGAACTTAGGCTTGAGCTTTGGACCAAAGGATGCTTTATTCCAGGTATACGAACAAAATGATTCTCTAGCAGCTGTTGCTTGTGGGCTTGGTGGAGGATCACTCATCAATGCAGGAGTTATGATACCAACACCAGTCCGTGCTAGATGTCATCCAAAATGGCCAAAGGAATGGGAAAGGAACTGGGATAATTGTGAAGCTTCTGCAACAGCCATGCTGAAAATACAAAGTACACCTGTCAAGTTTCCTTCTGCGAAAACCTTGGAAGAAGTTGCATATGGAGAGCTTGGAGAAACTTTTGAGATGTCAATGAAGCTGAGTGTCAATTTTGATATTGAAGAACCAATAACCAAGGGAATGAAGCCTCCACAGATGAGTAGCTGCTTAGCCTGTGGGAACTGTCTTTCTGGATGTCCTTACAATGCCAAAAGTTCTACAGACAAGAACTATATACTCTCAGCAATCCAGGAGGGATGCATTGTTAAGACAGAATGTCAAGTGAAGTATGTAGTGAGGAACCTGTATGAAAATTTCCCATGTGAAAGAAAGATTGTCAGGAAAAATAGAAGATGGCGTGTTTACTTCAATGAGATTGATTATATAACTTCTGATTTTGTAATTCTATCAGCTGGCGTGTTTGGCACAACTGAGATACTCTTCCAATCCCAAATGAGAGGACTGAAACTTTCAGAAGCACTTGGATCTGGATTCAGCTGTAATGGGAATACTGTGGCTTATCTTGCTGGAAGTCCAGCGCCATTGGGTGCTTATGGATTAGACAGAAAGCAGATGTTTAAGACTCCTTTTGAGAAAAGGCCAGGACCATCAATCACTTCATCTTACACCTCTTCACTGGGATTTACAATCCAGAGTGCTGTACTTCCAACAGCTTATCCATACCTGCTGTTTAAAGGGATTACAACATATGGGTGGCCTGCCGGATACTGGTTTTTTCATGGGATTATAGACAAGATAAAACATATTATAGGTTTCAAAGCATGCCAATCAATGGTCCTTATTGCACTAGGACATGATGAGAGTGATGGTAAGATCACATTAGAAAAGGGAACAAACAAAATCTGCTTCACTCCACCTCATGATCCCCTTCTTCCACGAAAAATTAAAGCTTTTCAAAGGCTGACTAAGAAATTAGGAGGATTTCTTTTTATGGCAAAATACCGAAGTGCATCAGTACATCTTCTAGGAGGGTGTAATGCATCATCAGATCCTTCACATGGTGTTTGCAACCCTGACGGGCAGGTTTTTGACCCAAAGTCACCTGCCACAATTCATCCCGGCCTGTATGTCTGTGATGCTTCTTTGATTCCATGTTCTGTCGGCATAAACCCATCTCTTACTATTGCTACTGTAGCTGAGCATGTGAGTAGGAACCTTGTGCAGGATGTTCTCATGTACAAGAGCAGGGAAGGTCTTGAATGTGTTCTTAAAACTCCTAATCAAGATACAGAATCCTTCATTGATAGGAAGGCTATAAATGGCCAGAGATCATTGGTCACAATTAGAGAAACTATGAGAGGTTATATTGGCGGTATGCCATGCACAGCTTATCTCATAATGAAAATGAACTCCCAGGGCCAGACGGGCTCTCCTAAATGGAAGTTGGGTAATGGAGAATCCCATCCCCTTCTAAGAGGGAAAGTTGGAGGGCATGTGGAAATGATATCTTTTGAGAAGGACAAGTTACATATTCTAGACGGGAATGTAAATTTGTGTGCAGTAGATGACAGAACTCCTTACACACAGTATATGCATTATCGCCTTCTTCTTGCAGCTTCTACTGGCTCAAGATATATTCTTGAGGGAAGAAAGATAATGAACCCTTATCTCTTTCCCGTATATGCTTGGAGGGAAATGACAACACTGTATGTGACATTTGCAAAAGTTGCAGAGAGGAACTCAAAGGAAGAGAAGACGATTTTGAAAGGGGAGCTTAATATTTCTATGACAGAGCTTCTTAAGAGCACCATAAGCCTTAAAGGTAACAATAGAGGAAGGTTCATATGCCTCCTATTGGGGTCTCTCTTAAGAACCTATTTTTTGCAGATACCTCGAGGGAACCACCAGGATTTAAATCTGTCAGTTTATCACATGAAGACTTATCCAAGTGGCACTCTTCATGAAATAAAAACAGAAGATGGATTCATTATCAGTTGCAAGCAATGGAAGCCCCACCATATTTTGTCAAAACTCAAACGAGATGAACATCAAAATCCTGTTCTCCTTCTTAATGGATATTCTACTGAGAGTTACTGGCTGCCAACAGAAGCCAATGACTTAGTAAGATATTTACTCGAAGAAGGGCATGATGCATGGCTTTTACAACCAAGATTCCACCCTCTGAATCCTTCAAACAATTTTACGATTGAAGATGTTGCAAGATTTGATATCCCTGCTGCAATAAATAAGATCCTTGAACTTCATGGACCAAGGGCCAAGGTGCATGTAGTTGCACATTGTGTTGGAGGGTTAGCCATACACATGGCAGTCATGGGAGGTCATGTATCTGCAACCCATGTAGCTTCTCTGTCTTGCACTAACTCTTCAATGTTCTTCAAGCTTAATGCTTTCTCCAGAGTCAAAATGTGGCTTCCTCTGATCCCAATATCAATGTTCATACTTGGAGAAGACAAAACAGTTCCTCTATTGGAAATATCAAAGCTCACCTCGCGGCACCACCTCCTGAGACGTATAGCCCGCTTCATACCCCGGTATGAGAGGTGCACCTGCAATGAATGTGTAGTTTTTTCCGGCATGTTTGGAAACACCTTTTGGCACGAAAATGTTACCCCCAGCATGCATCAGTGGTTGAACAAGCAAAGCTCAACAAGGCTTCCTATGTCAGCATTTCCCCACCTCAGGAAAATATGCAACTCTGGTTTTATTGTAGACTGCAATGGTTGCAACTCATACCTGATCCATCCAGAAAGAATGGCACTCCCAACCTTATATATATCGGGCGGGCGATCTCTCCTTGTGACTCCTCAGACTTCTTTCCTAGCGCATAAGTATATGAAGCTGCACCAACCTGGCTTTAGACATGAAAGGGTGGTTGTGGAGGGTTTCGGGCATTCAGATCTATTGATTGGAGAAGAGTCTCATAAGAAGGTGTTTCCTCACATTCTGTCCCATATTAGATTAGCTGATGAAGGAGAAATTTATACCAATAGAAACAGGTGCAGTAAAGAGGCATTGGATTGGGAAGCTGATCAGCACTATGAAGGCAGCTTTGGACCTTGGGGAACTTGGTTTTCTCCTTTTGTGGTTGTTTCGTTGTTTATTATGTTAGTTTCTTTGCTGATCCTATTGGTTTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GMC_oxred_C GMC_oxred_N

Protein Analysis

1186

Amino Acids

132.58

Weight (kDa)

8.79

Isoelectric Point (pI)

41.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GMC_oxred_C PF05199 351 - 418 4.2e-10 GMC oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 3 cut(s) 113, 1510, 2975
AbsI CCTCGAGG 1 cut(s) 2292
Acc36I ACCTGC 5 cut(s) 113, 1131, 1471, 1510, 2975
AccB1I GGYRCC 1 cut(s) 2913
AccB7I CCANNNNNTGG 1 cut(s) 1231
AccI GTMKAC 2 cut(s) 270, 3129
AccII CGCG 1 cut(s) 2911
AciI CCGC 5 cut(s) 109, 1770, 2911, 2938, 3201
AclWI GGATC 8 cut(s) 328, 931, 1316, 1442, 2644, 2833, 3151, 3535
AcoI YGGCCR 2 cut(s) 383, 1720
AcsI RAATTY 3 cut(s) 751, 1948, 3396
AdeI CACNNNGTG 2 cut(s) 1460, 2699
AfaI GTAC 4 cut(s) 454, 1101, 1416, 1637
AfiI CCNNNNNNNGG 7 cut(s) 160, 1167, 1231, 1457, 1621, 2254, 3099
AflII CTTAAG 2 cut(s) 2198, 2266
AhdI GACNNNNNGTC 2 cut(s) 714, 1495
AjnI CCWGG 6 cut(s) 264, 687, 1039, 1812, 2304, 3268
AjuI GAANNNNNNNTTGG 6 cut(s) 18, 50, 2995, 3027, 3453, 3485
Alw21I GWGCWC 2 cut(s) 2207, 2966
Alw26I GTCTC 5 cut(s) 171, 193, 2265, 2920, 3342
Alw44I GTGCAC 1 cut(s) 2962
AlwI GGATC 8 cut(s) 328, 931, 1316, 1442, 2644, 2833, 3151, 3535
Ama87I CYCGRG 1 cut(s) 2292
AoxI GGCC 8 cut(s) 151, 383, 1037, 1154, 1519, 1720, 1816, 2673
ApaLI GTGCAC 1 cut(s) 2962
ApeKI GCWGC 9 cut(s) 17, 101, 293, 603, 2012, 2497, 2636, 3259, 3465
ApoI RAATTY 3 cut(s) 751, 1948, 3396
Asp700I GAANNNNTTC 1 cut(s) 1662
AspLEI GCGC 2 cut(s) 979, 3244
AspS9I GGNCC 8 cut(s) 151, 245, 1043, 1234, 1816, 2666, 2673, 3473
AsuC2I CCSGG 2 cut(s) 1518, 2950
AsuHPI GGTGA 3 cut(s) 50, 1491, 2896
AvaI CYCGRG 1 cut(s) 2292
AvaII GGWCC 5 cut(s) 245, 1043, 1234, 2666, 3473
AxyI CCTNAGG 1 cut(s) 3098
BaeGI GKGCMC 1 cut(s) 2966
BalI TGGCCA 2 cut(s) 385, 1722
BanI GGYRCC 1 cut(s) 2913
BanII GRGCYC 1 cut(s) 1829
BbsI GAAGAC 3 cut(s) 2159, 2342, 2874
Bbv12I GWGCWC 2 cut(s) 2207, 2966
BbvI GCAGC 9 cut(s) 29, 113, 305, 590, 2024, 2484, 2623, 3246, 3477
BccI CCATC 8 cut(s) 35, 786, 1054, 1259, 1579, 1869, 2372, 3168
BcgI CGANNNNNNTGC 2 cut(s) 2273, 2307
BciT130I CCWGG 6 cut(s) 266, 689, 1041, 1814, 2306, 3270
BciVI GTATCC 1 cut(s) 1157
BclI TGATCA 1 cut(s) 3448
BcnI CCSGG 2 cut(s) 1518, 2950
BcoDI GTCTC 5 cut(s) 171, 193, 2265, 2920, 3342
BfaI CTAG 6 cut(s) 290, 366, 1247, 1424, 1934, 3239
BfmI CTRYAG 3 cut(s) 21, 657, 1590
BfoI RGCGCY 1 cut(s) 980
BfrI CTTAAG 2 cut(s) 2198, 2266
BfuAI ACCTGC 5 cut(s) 113, 1131, 1471, 1510, 2975
BfuI GTATCC 1 cut(s) 1157
BglI GCCNNNNNGGC 1 cut(s) 1824
BglII AGATCT 1 cut(s) 3316
BlpI GCTNAGC 2 cut(s) 607, 1596
Bme1390I CCNGG 8 cut(s) 266, 689, 1041, 1518, 1814, 2306, 2950, 3270
Bme18I GGWCC 5 cut(s) 245, 1043, 1234, 2666, 3473
BmeRI GACNNNNNGTC 2 cut(s) 714, 1495
BmeT110I CYCGRG 1 cut(s) 2292
BmgT120I GGNCC 8 cut(s) 151, 245, 1043, 1234, 1816, 2666, 2673, 3473
BmiI GGNNCC 4 cut(s) 740, 1613, 2300, 2915
BmrFI CCNGG 8 cut(s) 266, 689, 1041, 1518, 1814, 2306, 2950, 3270
BmrI ACTGGG 2 cut(s) 415, 1085
BmsI GCATC 8 cut(s) 52, 244, 686, 1418, 1448, 1525, 2539, 3049
BmuI ACTGGG 2 cut(s) 415, 1085
BpiI GAAGAC 3 cut(s) 2159, 2342, 2874
BplI GAGNNNNNCTC 4 cut(s) 309, 341, 2123, 2155
BpmI CTGGAG 1 cut(s) 2797
Bpu1102I GCTNAGC 2 cut(s) 607, 1596
BpuEI CTTGAG 3 cut(s) 256, 2011, 2060
BpuMI CCSGG 2 cut(s) 1518, 2950
BsaBI GATNNNNATC 2 cut(s) 2313, 2445
BsaI GGTCTC 1 cut(s) 2265
BsaJI CCNNGG 8 cut(s) 483, 573, 1812, 1813, 2669, 2676, 2948, 3476
BsaXI ACNNNNNCTCC 6 cut(s) 1813, 1843, 1878, 1908, 2727, 2757
Bsc4I CCNNNNNNNGG 7 cut(s) 160, 1167, 1231, 1457, 1621, 2254, 3099
Bse1I ACTGG 6 cut(s) 356, 410, 1080, 1172, 2026, 2498
Bse21I CCTNAGG 1 cut(s) 3098
Bse3DI GCAATG 3 cut(s) 2408, 2977, 3142
Bse8I GATNNNNATC 2 cut(s) 2313, 2445
BseBI CCWGG 6 cut(s) 266, 689, 1041, 1814, 2306, 3270
BseDI CCNNGG 8 cut(s) 483, 573, 1812, 1813, 2669, 2676, 2948, 3476
BseGI GGATG 8 cut(s) 259, 373, 638, 701, 1513, 1630, 1861, 3160
BseJI GATNNNNATC 2 cut(s) 2313, 2445
BseLI CCNNNNNNNGG 7 cut(s) 160, 1167, 1231, 1457, 1621, 2254, 3099
BseMI GCAATG 3 cut(s) 2408, 2977, 3142
BseMII CTCAG 9 cut(s) 182, 530, 693, 861, 1587, 2475, 2915, 3112, 3239
BseNI ACTGG 6 cut(s) 356, 410, 1080, 1172, 2026, 2498
BseRI GAGGAG 1 cut(s) 3213
BseSI GKGCMC 1 cut(s) 2966
BseXI GCAGC 9 cut(s) 29, 113, 305, 590, 2024, 2484, 2623, 3246, 3477
BseYI CCCAGC 1 cut(s) 3032
BsgI GTGCAG 4 cut(s) 1640, 1977, 3245, 3439
Bsh1236I CGCG 1 cut(s) 2911
BshFI GGCC 8 cut(s) 153, 385, 1039, 1156, 1521, 1722, 1818, 2675
BshNI GGYRCC 1 cut(s) 2913
BsiHKAI GWGCWC 2 cut(s) 2207, 2966
BsiHKCI CYCGRG 1 cut(s) 2292
BsiSI CCGG 5 cut(s) 154, 1161, 1518, 2950, 2991
BslFI GGGAC 1 cut(s) 3352
BslI CCNNNNNNNGG 7 cut(s) 160, 1167, 1231, 1457, 1621, 2254, 3099
BsmAI GTCTC 5 cut(s) 171, 193, 2265, 2920, 3342
BsmBI CGTCTC 1 cut(s) 2920
BsmFI GGGAC 1 cut(s) 3352
BsmI GAATGC 1 cut(s) 3310
BsnI GGCC 8 cut(s) 153, 385, 1039, 1156, 1521, 1722, 1818, 2675
Bso31I GGTCTC 1 cut(s) 2265
BsoBI CYCGRG 1 cut(s) 2292
Bsp1286I GDGCHC 3 cut(s) 1829, 2207, 2966
Bsp1407I TGTACA 1 cut(s) 1635
Bsp1720I GCTNAGC 2 cut(s) 607, 1596
BspACI CCGC 5 cut(s) 109, 1770, 2911, 2938, 3201
BspANI GGCC 8 cut(s) 153, 385, 1039, 1156, 1521, 1722, 1818, 2675
BspCNI CTCAG 9 cut(s) 181, 531, 692, 862, 1588, 2476, 2916, 3111, 3238
BspFNI CGCG 1 cut(s) 2911
BspHI TCATGA 2 cut(s) 1318, 2359
BspLI GGNNCC 4 cut(s) 740, 1613, 2300, 2915
BspMI ACCTGC 5 cut(s) 113, 1131, 1471, 1510, 2975
BspPI GGATC 8 cut(s) 328, 931, 1316, 1442, 2644, 2833, 3151, 3535
BspT107I GGYRCC 1 cut(s) 2913
BspTI CTTAAG 2 cut(s) 2198, 2266
BspTNI GGTCTC 1 cut(s) 2265
BsrDI GCAATG 3 cut(s) 2408, 2977, 3142
BsrGI TGTACA 1 cut(s) 1635
BsrI ACTGG 6 cut(s) 356, 410, 1080, 1172, 2026, 2498
BssECI CCNNGG 8 cut(s) 483, 573, 1812, 1813, 2669, 2676, 2948, 3476
BssNAI GTATAC 1 cut(s) 271
BssT1I CCWWGG 5 cut(s) 483, 573, 2669, 2676, 3476
Bst1107I GTATAC 1 cut(s) 271
Bst2UI CCWGG 6 cut(s) 266, 689, 1041, 1814, 2306, 3270
Bst4CI ACNGT 6 cut(s) 623, 952, 1591, 1986, 2106, 2878
Bst6I CTCTTC 6 cut(s) 884, 1075, 2142, 2361, 2788, 3327
BstAFI CTTAAG 2 cut(s) 2198, 2266
BstAPI GCANNNNNTGC 3 cut(s) 299, 2126, 3141
BstAUI TGTACA 1 cut(s) 1635
BstC8I GCNNGC 8 cut(s) 106, 853, 1158, 1222, 2399, 2938, 3038, 3203
BstENI CCTNNNNNAGG 1 cut(s) 1619
BstF5I GGATG 8 cut(s) 259, 373, 638, 701, 1513, 1630, 1861, 3160
BstFNI CGCG 1 cut(s) 2911
BstH2I RGCGCY 1 cut(s) 980
BstHHI GCGC 2 cut(s) 979, 3244
BstMAI GTCTC 5 cut(s) 171, 193, 2265, 2920, 3342
BstNI CCWGG 6 cut(s) 266, 689, 1041, 1814, 2306, 3270
BstNSI RCATGY 6 cut(s) 1224, 1604, 1895, 2687, 2998, 3040
BstSCI CCNGG 8 cut(s) 264, 687, 1039, 1516, 1812, 2304, 2948, 3268
BstSFI CTRYAG 3 cut(s) 21, 657, 1590
BstSLI GKGCMC 1 cut(s) 2966
BstUI CGCG 1 cut(s) 2911
BstV1I GCAGC 9 cut(s) 29, 113, 305, 590, 2024, 2484, 2623, 3246, 3477
BstV2I GAAGAC 3 cut(s) 2159, 2342, 2874
BstX2I RGATCY 4 cut(s) 923, 1447, 2649, 3316
BstXI CCANNNNNNTGG 2 cut(s) 393, 2722
BstYI RGATCY 4 cut(s) 923, 1447, 2649, 3316
BstZ17I GTATAC 1 cut(s) 271
Bsu36I CCTNAGG 1 cut(s) 3098
BsuI GTATCC 1 cut(s) 1157
BsuRI GGCC 8 cut(s) 153, 385, 1039, 1156, 1521, 1722, 1818, 2675
BtsCI GGATG 8 cut(s) 259, 373, 638, 701, 1513, 1630, 1861, 3160
BtsIMutI CAGTG 3 cut(s) 1073, 2102, 3050
BveI ACCTGC 5 cut(s) 113, 1131, 1471, 1510, 2975
Cac8I GCNNGC 8 cut(s) 106, 853, 1158, 1222, 2399, 2938, 3038, 3203
CciI TCATGA 2 cut(s) 1318, 2359
CfoI GCGC 2 cut(s) 979, 3244
Cfr13I GGNCC 8 cut(s) 151, 245, 1043, 1234, 1816, 2666, 2673, 3473
Csp6I GTAC 4 cut(s) 453, 1100, 1415, 1636
CviQI GTAC 4 cut(s) 453, 1100, 1415, 1636
DraI TTTAAA 2 cut(s) 1132, 2313
DraIII CACNNNGTG 2 cut(s) 1460, 2699
DriI GACNNNNNGTC 2 cut(s) 714, 1495
EaeI YGGCCR 2 cut(s) 383, 1720
Eam1104I CTCTTC 6 cut(s) 884, 1075, 2142, 2361, 2788, 3327
Eam1105I GACNNNNNGTC 2 cut(s) 714, 1495
EarI CTCTTC 6 cut(s) 884, 1075, 2142, 2361, 2788, 3327
Eco130I CCWWGG 5 cut(s) 483, 573, 2669, 2676, 3476
Eco24I GRGCYC 1 cut(s) 1829
Eco31I GGTCTC 1 cut(s) 2265
Eco32I GATATC 2 cut(s) 1905, 2629
Eco47I GGWCC 5 cut(s) 245, 1043, 1234, 2666, 3473
Eco81I CCTNAGG 1 cut(s) 3098
Eco88I CYCGRG 1 cut(s) 2292
EcoNI CCTNNNNNAGG 1 cut(s) 1619
EcoRII CCWGG 6 cut(s) 264, 687, 1039, 1812, 2304, 3268
EcoRV GATATC 2 cut(s) 1905, 2629
EcoT14I CCWWGG 5 cut(s) 483, 573, 2669, 2676, 3476
EcoT22I ATGCAT 5 cut(s) 701, 1441, 1995, 2554, 3042
EcoT38I GRGCYC 1 cut(s) 1829
ErhI CCWWGG 5 cut(s) 483, 573, 2669, 2676, 3476
Esp3I CGTCTC 1 cut(s) 2920
FalI AAGNNNNNCTT 2 cut(s) 2157, 2189
FaqI GGGAC 1 cut(s) 3352
FauI CCCGC 2 cut(s) 2945, 3194
FauNDI CATATG 3 cut(s) 499, 1147, 2243
FbaI TGATCA 1 cut(s) 3448
FblI GTMKAC 2 cut(s) 270, 3129
FokI GGATG 8 cut(s) 266, 360, 645, 708, 1500, 1637, 1848, 3147
FriOI GRGCYC 1 cut(s) 1829
FspBI CTAG 6 cut(s) 290, 366, 1247, 1424, 1934, 3239
GlaI GCGC 2 cut(s) 978, 3243
GsaI CCCAGC 1 cut(s) 3036
GsuI CTGGAG 1 cut(s) 2797
HaeII RGCGCY 1 cut(s) 980
HaeIII GGCC 8 cut(s) 153, 385, 1039, 1156, 1521, 1722, 1818, 2675
HapII CCGG 5 cut(s) 154, 1161, 1518, 2950, 2991
HhaI GCGC 2 cut(s) 979, 3244
Hin6I GCGC 2 cut(s) 977, 3242
HinP1I GCGC 2 cut(s) 977, 3242
HindIII AAGCTT 3 cut(s) 420, 1347, 2798
HpaII CCGG 5 cut(s) 154, 1161, 1518, 2950, 2991
HphI GGTGA 3 cut(s) 50, 1491, 2896
Hpy166II GTNNAC 5 cut(s) 271, 455, 802, 2964, 3130
Hpy188I TCNGA 9 cut(s) 141, 212, 832, 913, 1447, 2583, 2838, 3228, 3316
Hpy8I GTNNAC 5 cut(s) 271, 455, 802, 2964, 3130
HpyCH4III ACNGT 6 cut(s) 623, 952, 1591, 1986, 2106, 2878
HpyCH4IV ACGT 1 cut(s) 2929
HpySE526I ACGT 1 cut(s) 2929
HspAI GCGC 2 cut(s) 977, 3242
Ksp22I TGATCA 1 cut(s) 3448
LmnI GCTCC 1 cut(s) 2170
Lsp1109I GCAGC 9 cut(s) 29, 113, 305, 590, 2024, 2484, 2623, 3246, 3477
LweI GCATC 8 cut(s) 52, 244, 686, 1418, 1448, 1525, 2539, 3049
MaeI CTAG 6 cut(s) 290, 366, 1247, 1424, 1934, 3239
MaeII ACGT 1 cut(s) 2929
MaeIII GTNAC 8 cut(s) 1497, 1735, 1923, 2110, 2222, 2489, 3025, 3217
MflI RGATCY 4 cut(s) 923, 1447, 2649, 3316
MhlI GDGCHC 3 cut(s) 1829, 2207, 2966
MlsI TGGCCA 2 cut(s) 385, 1722
MluNI TGGCCA 2 cut(s) 385, 1722
MlyI GAGTC 5 cut(s) 173, 1015, 2826, 3214, 3344
MmeI TCCRAC 4 cut(s) 203, 1130, 1864, 2683
Mox20I TGGCCA 2 cut(s) 385, 1722
Mph1103I ATGCAT 5 cut(s) 701, 1441, 1995, 2554, 3042
MroXI GAANNNNTTC 1 cut(s) 1662
MscI TGGCCA 2 cut(s) 385, 1722
MslI CAYNNNNRTG 3 cut(s) 1150, 2720, 3021
Msp20I TGGCCA 2 cut(s) 385, 1722
MspA1I CMGCKG 4 cut(s) 20, 296, 851, 936
MspCI CTTAAG 2 cut(s) 2198, 2266
MspI CCGG 5 cut(s) 154, 1161, 1518, 2950, 2991
MspR9I CCNGG 8 cut(s) 266, 689, 1041, 1518, 1814, 2306, 2950, 3270
Mva1269I GAATGC 1 cut(s) 3310
MvaI CCWGG 6 cut(s) 266, 689, 1041, 1814, 2306, 3270
MvnI CGCG 1 cut(s) 2911
NciI CCSGG 2 cut(s) 1518, 2950
NdeI CATATG 3 cut(s) 499, 1147, 2243
NlaIV GGNNCC 4 cut(s) 740, 1613, 2300, 2915
NmuCI GTSAC 4 cut(s) 1497, 1735, 2110, 3217
NsiI ATGCAT 5 cut(s) 701, 1441, 1995, 2554, 3042
NspI RCATGY 6 cut(s) 1224, 1604, 1895, 2687, 2998, 3040
PaeI GCATGC 2 cut(s) 1224, 3040
PaeR7I CTCGAG 1 cut(s) 2292
PagI TCATGA 2 cut(s) 1318, 2359
PaqCI CACCTGC 3 cut(s) 113, 1510, 2975
PasI CCCWGGG 1 cut(s) 1813
PctI GAATGC 1 cut(s) 3310
PdmI GAANNNNTTC 1 cut(s) 1662
PfeI GAWTC 8 cut(s) 284, 930, 1546, 1691, 1856, 2382, 2571, 2584
PflMI CCANNNNNTGG 1 cut(s) 1231
PfoI TCCNGGA 1 cut(s) 687
PleI GAGTC 5 cut(s) 172, 1015, 2825, 3214, 3343
PpsI GAGTC 5 cut(s) 172, 1015, 2825, 3214, 3343
Psp6I CCWGG 6 cut(s) 264, 687, 1039, 1812, 2304, 3268
PspFI CCCAGC 1 cut(s) 3032
PspGI CCWGG 6 cut(s) 264, 687, 1039, 1812, 2304, 3268
PspN4I GGNNCC 4 cut(s) 740, 1613, 2300, 2915
PspPI GGNCC 8 cut(s) 151, 245, 1043, 1234, 1816, 2666, 2673, 3473
PspXI VCTCGAGB 1 cut(s) 2292
PsuI RGATCY 4 cut(s) 923, 1447, 2649, 3316
PvuII CAGCTG 4 cut(s) 20, 296, 851, 936
RsaI GTAC 4 cut(s) 454, 1101, 1416, 1637
RsaNI GTAC 4 cut(s) 453, 1100, 1415, 1636
RseI CAYNNNNRTG 3 cut(s) 1150, 2720, 3021
Sau96I GGNCC 8 cut(s) 151, 245, 1043, 1234, 1816, 2666, 2673, 3473
SchI GAGTC 5 cut(s) 173, 1015, 2826, 3214, 3344
ScrFI CCNGG 8 cut(s) 266, 689, 1041, 1518, 1814, 2306, 2950, 3270
SduI GDGCHC 3 cut(s) 1829, 2207, 2966
SfaNI GCATC 8 cut(s) 52, 244, 686, 1418, 1448, 1525, 2539, 3049
SfcI CTRYAG 3 cut(s) 21, 657, 1590
Sfr274I CTCGAG 1 cut(s) 2292
SinI GGWCC 5 cut(s) 245, 1043, 1234, 2666, 3473
SlaI CTCGAG 1 cut(s) 2292
SmiI ATTTAAAT 1 cut(s) 2313
SmiMI CAYNNNNRTG 3 cut(s) 1150, 2720, 3021
SmlI CTYRAG 6 cut(s) 235, 2026, 2039, 2198, 2266, 2292
SmoI CTYRAG 6 cut(s) 235, 2026, 2039, 2198, 2266, 2292
SphI GCATGC 2 cut(s) 1224, 3040
SsiI CCGC 5 cut(s) 109, 1770, 2911, 2938, 3201
SspI AATATT 1 cut(s) 2179
SspMI CTAG 6 cut(s) 290, 366, 1247, 1424, 1934, 3239
StyD4I CCNGG 8 cut(s) 264, 687, 1039, 1516, 1812, 2304, 2948, 3268
StyI CCWWGG 5 cut(s) 483, 573, 2669, 2676, 3476
SwaI ATTTAAAT 1 cut(s) 2313
TaaI ACNGT 6 cut(s) 623, 952, 1591, 1986, 2106, 2878
TaiI ACGT 1 cut(s) 2932
TaqI TCGA 2 cut(s) 2293, 2535
TatI WGTACW 4 cut(s) 452, 1099, 1414, 1635
TauI GCSGC 2 cut(s) 111, 2914
TfiI GAWTC 8 cut(s) 284, 930, 1546, 1691, 1856, 2382, 2571, 2584
TscAI CASTG 3 cut(s) 1080, 2109, 3050
TseFI GTSAC 4 cut(s) 1497, 1735, 2110, 3217
TseI GCWGC 9 cut(s) 17, 101, 293, 603, 2012, 2497, 2636, 3259, 3465
Tsp45I GTSAC 4 cut(s) 1497, 1735, 2110, 3217
TspGWI ACGGA 1 cut(s) 350
TspRI CASTG 3 cut(s) 1080, 2109, 3050
Van91I CCANNNNNTGG 1 cut(s) 1231
Vha464I CTTAAG 2 cut(s) 2198, 2266
VneI GTGCAC 1 cut(s) 2962
VpaK11BI GGWCC 5 cut(s) 245, 1043, 1234, 2666, 3473
XagI CCTNNNNNAGG 1 cut(s) 1619
XapI RAATTY 3 cut(s) 751, 1948, 3396
XbaI TCTAGA 1 cut(s) 1933
XceI RCATGY 6 cut(s) 1224, 1604, 1895, 2687, 2998, 3040
XhoI CTCGAG 1 cut(s) 2292
XmiI GTMKAC 2 cut(s) 270, 3129
XmnI GAANNNNTTC 1 cut(s) 1662
XspI CTAG 6 cut(s) 290, 366, 1247, 1424, 1934, 3239
Zsp2I ATGCAT 5 cut(s) 701, 1441, 1995, 2554, 3042
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.