MD10G1176100.v1.1

GMC oxidoreductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0003277
Physical Location & Seq
Forward (+)
26845060 .. 26852392
7333 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1176100.v1.1.491

Sequence Viewer

Length: 3552 bp
ATGAAGAAATTACAAGGAAGTAGTTTTGGTGATGGGGAAGCCGAAGAGTATGATGTGGTTGTTGTGGGGTCGGGCTACGGTGGTTCTGTGGTTGCCTGCAGGTTGTCGATGGCCGGCGGCAGAAGCGTTTGTCTGCTTGAGAAAGGCCGCAGATGGGAAGCTAAGGATTTCCCAACAGACAGCTTCAAGTTGTTATCAGCTTCGAGGGTTGAAAGCCGGAACTTAGGCCTTAGCCTTGGATCCAAAGATGCACTAATCCAGGTTCTATTTGGACACAAGGCCCAACCTTCAAAGCTCACTGTCAATCCGGCTTACCGGAAAAATTGGGCGAAGTGTGGAACGATTCACGTCGGAACAAGGGTGTGCATTTTTCAGTTTGGTGTGATGTCTTCCTTCGTGGAGGTAGCAGCAGTTGTGGCGGTGGTGACTGATTTTTGGCGCTTGGAGCTTAGGGTTTTACTGTTCACTGTTTCTGTTGTATATGAGCAAAACGATTCTGTAGCAGTAGTGGGTTGCGGACTCGGTGGAGGTTCACTAGTGAATGCAGGAGTTATGATGCCGACACCAGTTCGAGCCAGACGGCATCCAAAATGGCCAAAGCAATGGGAAAAGAATTGGGATAGTTGTGAAGCTTCTGCAGCTGCCATGCTCAAAGTACAAAGCCTTCCTGACAAGTTTTCTGTTGCGAAAGTCTTGGAAGATCATATAACTAGTGAAGAAACTTTTGAGACTCCGGTGAAGCTGACTATGAATTTTGACTTGGAAGATGGTCAACGTCCACTATCCAATAATAATCCAATTAAGAAGGTTCAACAGATGAGAGGAGTTTTTGGCACGACTGAGATCCTCTTTCAGTCACAAATGAGAGGACTAAGAGTTTCAGAAACACTTGGTTCAGGATTCAGCTGCAACGGAAACACTGTTGCCTATCTCGCGGGAAGCCCGATGCCGTTGAATGGTTATGGACTAGATAAGAAGCAACTCTTTAAGATTCCATTTCAAGCAAGGCCAGGACCATCCATCTCATCATCGTACACTTCTTCGCTGGGGTTTACAATTCAGAGTGCAATACTTCCATCAGCTTATCCGAACCTTATGTTTAAAGGAATTGTGACTTATGGATGGCCTGCCGGGTACTGGTTCTTTCATGGGATCATTGACAAGATAAAGCTTGCAATGGGTTTTAAAGCTACCCATGCAGTGGCTCTTCTTGCGTTGGGATATGATGAGAGTGATGGCAAAATCATGTTAGAAAGGGGCACCAACAAAATCAGCTTTAATCCACCTCGTGATCCCCTTCTTGCCCGAAAAATTACAGCTTTTCAAAAGCTCACTAAGAAATTAGGAGGAGTTCTCTTCATGTCGAAGTACCGAAGCACAGCTGTTCATCTTTTAGGTGGGTGCAATGCATCATCAAGTCCGTTACATGGTGTTTGCAACCATAATGGGCAGGTTTTTGACCCAGCTCATGATGAGTTACCTGCCGCTGCAGTACACCCCGGCCTATATGTGTGTGATGCTTCTTTGATTCCATGTTCTGTTGGCATAAATCCATCTCTTACTATTGCCACTGCAGCTGAGCACATAAGTAGGCAACTTGTGAAGGATGTTCTCGAGTACAGCACCAACAATATCGACAATAAAGGTCCAGATTCCCTCACTGATAAAACAAAGACTATAGCTATAGGTAATGGTAAAAAATCAGATCTCGCGTTCAAAGAAACCATGAGAGGACATGTTGGGGGTATGCCATGCACAGCTTATCTCAAAATGAAGATGAACCCTAATGATGAGAACTATATGGACTATGACAAGTTGGGTACCAATATTCGCCGTGAGTCCCATCCCCTTCTAAGAGGGAAAGTTGGAGGGCACGTAGAACTTAGAGGCTTTGAGAAGGATAATTTGCATATCATAGATGGAGACGTAAATTTGTGTGAAGTAGATTGCAGAACTCCTTACACACAATATATGCTTTATCGTCTTTGCCTTGTGGCTTCTACTGGTTCAAGGTATATTCTGCAGGGGAGAAAGATTATGAATCCTTATCTCTTTGCATTATATGCTTGGAGGGAAACGACCACAATGCATGTAACATTTGAAAAAGTTCCCGAGAAAAACTCAGTGGATCATGATCATGAGAATGTGATCTTAAAAGGGGAGCTTAGTATTTCCATGACGGAGCTTCTTAAGAGCTTGGTGAGCTTCGAAGGAAACAAGAAAATAAAGTTCTTATGCCTCCTATCAGGGACTTTCTTGAGAACCTATTTCTTGAAGATACCTCGAGGGAACCAAGAGTACTTTAATTTGTTAGACTGTGAACATAAATATTCTTATCCAAGCAGCACCCTGCACGATATAGAAACAGAAGATGGAGTCGTGATCAGTTGCAGGCAATGGAATTGCCATGATTTGTCAAAATTTAGAGGATCAGATGGACAACGAAACCCGATTCTCCTTGTTAATGGTTATGCTGTTGAGAGTTACTGGCTTCCGACGGAGCCAAATGACTTGATCAGAAGTTTACTTGAAAAAGGGCATGAAACATGGTTATTACAATCCAGGCTGCACGCTCGTACTCCTCCAAACACTTTTACCCTAGAAGATGTTGGAAGATTTGATATCCCTGCAGCAATTAATAAGATGCTTGAATTGCTTGGACCGAGCGTAAAGGTGCATGTAGTTGCACACTGTGTTGGAGGCTTAGCTATTCACATAGCTCTCATGGGAGGTCATGTCTGTGGAAACCATATAGCTTCTCTGTCTTGCACCAACTCTTCAATGTTCTTCAAGCTTAATGCTTTGTCCAAAGTCAAAATGTGGCTTCCTATACTGCCAATATCCATGTTGATACTTGGAAATAACAAGACACTTCCGCTTGTGGAAACATCAACGCCAGTGAGCTTACGTCATAGAATCTTAAAACTTGTAGCGCGCTTGATACCGCGTTACGAGAGATGCACCTGTAATGAATGTGAAGTTGTTTCCGGCATATTTGGAAACGCATTTTGGCACGAAAATATAAGCCCAACTGTGCACCAGTGGTTGAACAAGGAAAGCTCAAAAAGGCTTCCTATGGCAGCATTTCCCCACCTCAGAAAGATATGCAAATCTGGTTTTATATTAGACAGCAATGGTAATAACTCCTACTTGATCCACCCAGAAAGAATGGCACTCCCAACACTTTATATATCGGGCGGGCGGTCTCTTCTTGTGACTCCTCAAACATCTTTTCTTGCTCATAAATACATGAAGTTGCACCAACCAGGGTTTAGACATGAAAGGGTAGTTGTGGAGGGTTTTGGGCATTCAGATTTGCTGATTGGAGAAGAGTCTTGCAAGAAGGTCTTTCCTCACATTTTATCTCATATAAGAGTAGCTGAGGAAAAGAAATACTACTGCAGGAAAGAGGCCTCGGATTCAGAAGCAGATCATTATCAATATGAGGAAGGATTTGGAGAATTTGGAACTTGGTTTTCTCCTTTTATTATTCTTCTGCTAGCCTTTATATCCCTTTCTTTGTTGGTCCGATTATTTTTACTATCAAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GMC_oxred_C GMC_oxred_N

Protein Analysis

1184

Amino Acids

131.61

Weight (kDa)

8.88

Isoelectric Point (pI)

42.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GMC_oxred_C PF05199 454 - 526 3.7e-07 GMC oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 2283
Acc36I ACCTGC 3 cut(s) 90, 1441, 1489
Acc65I GGTACC 1 cut(s) 1820
AccB1I GGYRCC 2 cut(s) 1259, 1820
AccB7I CCANNNNNTGG 1 cut(s) 1201
AccII CGCG 4 cut(s) 935, 1712, 2935, 2947
AclWI GGATC 8 cut(s) 234, 247, 838, 1160, 1286, 2136, 2437, 3148
AcoI YGGCCR 2 cut(s) 111, 593
AcsI RAATTY 4 cut(s) 751, 1930, 2420, 3461
AdeI CACNNNGTG 2 cut(s) 1289, 2693
AfaI GTAC 9 cut(s) 657, 1034, 1136, 1370, 1494, 1619, 1822, 2300, 2578
AfiI CCNNNNNNNGG 5 cut(s) 154, 956, 1137, 1201, 1427
AflII CTTAAG 1 cut(s) 2189
AflIII ACRYGT 1 cut(s) 1735
AhlI ACTAGT 2 cut(s) 535, 710
AjiI CACGTC 1 cut(s) 349
AjnI CCWGG 4 cut(s) 258, 1009, 2561, 3265
AjuI GAANNNNNNNTTGG 4 cut(s) 743, 775, 2992, 3024
Alw21I GWGCWC 2 cut(s) 1584, 3039
Alw26I GTCTC 3 cut(s) 722, 1917, 3210
Alw44I GTGCAC 1 cut(s) 3035
AlwI GGATC 8 cut(s) 234, 247, 838, 1160, 1286, 2136, 2437, 3148
Ama87I CYCGRG 3 cut(s) 1613, 2111, 2283
AoxI GGCC 9 cut(s) 111, 145, 226, 279, 593, 1007, 1124, 1501, 3411
ApaLI GTGCAC 1 cut(s) 3035
ApoI RAATTY 4 cut(s) 751, 1930, 2420, 3461
AseI ATTAAT 1 cut(s) 2637
Asp700I GAANNNNTTC 1 cut(s) 2106
Asp718I GGTACC 1 cut(s) 1820
AspLEI GCGC 3 cut(s) 441, 2935, 2937
AspS9I GGNCC 5 cut(s) 280, 1013, 1646, 2660, 3526
AsuC2I CCSGG 2 cut(s) 1132, 1500
AsuHPI GGTGA 4 cut(s) 41, 436, 748, 2212
AsuII TTCGAA 1 cut(s) 2208
AsuNHI GCTAGC 1 cut(s) 3499
AvaI CYCGRG 3 cut(s) 1613, 2111, 2283
AvaII GGWCC 4 cut(s) 1013, 1646, 2660, 3526
BaeGI GKGCMC 3 cut(s) 1262, 1875, 3039
BalI TGGCCA 1 cut(s) 595
BamHI GGATCC 1 cut(s) 239
BanI GGYRCC 2 cut(s) 1259, 1820
BarI GAAGNNNNNNTAC 2 cut(s) 648, 680
BauI CACGAG 1 cut(s) 1287
BbsI GAAGAC 1 cut(s) 381
Bbv12I GWGCWC 2 cut(s) 1584, 3039
BbvCI CCTCAGC 1 cut(s) 3381
BceAI ACGGC 3 cut(s) 596, 934, 1818
BcgI CGANNNNNNTGC 2 cut(s) 2068, 2102
BciT130I CCWGG 4 cut(s) 260, 1011, 2563, 3267
BclI TGATCA 3 cut(s) 2134, 2382, 2514
BcnI CCSGG 2 cut(s) 1132, 1500
BcoDI GTCTC 3 cut(s) 722, 1917, 3210
BcuI ACTAGT 2 cut(s) 535, 710
BfaI CTAG 5 cut(s) 536, 711, 968, 2600, 3500
BfoI RGCGCY 1 cut(s) 442
BfrI CTTAAG 1 cut(s) 2189
BfuAI ACCTGC 3 cut(s) 90, 1441, 1489
BglII AGATCT 1 cut(s) 1705
BlpI GCTNAGC 2 cut(s) 1578, 2704
BmcAI AGTACT 1 cut(s) 2300
Bme1390I CCNGG 6 cut(s) 260, 1011, 1132, 1500, 2563, 3267
Bme18I GGWCC 4 cut(s) 1013, 1646, 2660, 3526
BmeT110I CYCGRG 3 cut(s) 1613, 2111, 2283
BmgBI CACGTC 1 cut(s) 349
BmgT120I GGNCC 5 cut(s) 280, 1013, 1646, 2660, 3526
BmiI GGNNCC 5 cut(s) 241, 1261, 1822, 2291, 2502
BmrFI CCNGG 6 cut(s) 260, 1011, 1132, 1500, 2563, 3267
BmsI GCATC 8 cut(s) 238, 546, 592, 936, 1418, 1507, 2634, 2948
BmtI GCTAGC 1 cut(s) 3503
BpiI GAAGAC 1 cut(s) 381
BplI GAGNNNNNCTC 4 cut(s) 1338, 1370, 2105, 2137
Bpu10I CCTNAGC 4 cut(s) 162, 230, 449, 3381
Bpu1102I GCTNAGC 2 cut(s) 1578, 2704
Bpu14I TTCGAA 1 cut(s) 2208
BpuEI CTTGAG 2 cut(s) 158, 2278
BpuMI CCSGG 2 cut(s) 1132, 1500
BsaAI YACGTR 1 cut(s) 1876
BsaBI GATNNNNATC 2 cut(s) 2133, 3435
BsaI GGTCTC 1 cut(s) 3210
BsaJI CCNNGG 4 cut(s) 235, 1498, 3266, 3414
BsaWI WCCGGW 2 cut(s) 315, 733
BsaXI ACNNNNNCTCC 4 cut(s) 1860, 1890, 2721, 2751
Bsc4I CCNNNNNNNGG 5 cut(s) 154, 956, 1137, 1201, 1427
Bse118I RCCGGY 1 cut(s) 113
Bse1I ACTGG 6 cut(s) 566, 1142, 2008, 2492, 2897, 3040
Bse3DI GCAATG 5 cut(s) 608, 1182, 1411, 2402, 3139
Bse8I GATNNNNATC 2 cut(s) 2133, 3435
BseBI CCWGG 4 cut(s) 260, 1011, 2563, 3267
BseDI CCNNGG 4 cut(s) 235, 1498, 3266, 3414
BseGI GGATG 5 cut(s) 583, 1016, 1127, 1612, 1843
BseJI GATNNNNATC 2 cut(s) 2133, 3435
BseLI CCNNNNNNNGG 5 cut(s) 154, 956, 1137, 1201, 1427
BseMI GCAATG 5 cut(s) 608, 1182, 1411, 2402, 3139
BseMII CTCAG 5 cut(s) 831, 1569, 2136, 3109, 3372
BseNI ACTGG 6 cut(s) 566, 1142, 2008, 2492, 2897, 3040
BsePI GCGCGC 1 cut(s) 2933
BseRI GAGGAG 4 cut(s) 837, 1362, 2571, 3210
BseSI GKGCMC 3 cut(s) 1262, 1875, 3039
BseYI CCCAGC 2 cut(s) 1045, 1462
BsgI GTGCAG 2 cut(s) 2336, 2552
Bsh1236I CGCG 4 cut(s) 935, 1712, 2935, 2947
BshFI GGCC 9 cut(s) 113, 147, 228, 281, 595, 1009, 1126, 1503, 3413
BshNI GGYRCC 2 cut(s) 1259, 1820
BsiHKAI GWGCWC 2 cut(s) 1584, 3039
BsiHKCI CYCGRG 3 cut(s) 1613, 2111, 2283
BsiSI CCGG 8 cut(s) 114, 217, 308, 316, 734, 1131, 1500, 2988
BslFI GGGAC 2 cut(s) 1825, 2263
BslI CCNNNNNNNGG 5 cut(s) 154, 956, 1137, 1201, 1427
BsmAI GTCTC 3 cut(s) 722, 1917, 3210
BsmBI CGTCTC 1 cut(s) 1917
BsmFI GGGAC 2 cut(s) 1825, 2263
BsmI GAATGC 2 cut(s) 547, 3307
BsnI GGCC 9 cut(s) 113, 147, 228, 281, 595, 1009, 1126, 1503, 3413
Bso31I GGTCTC 1 cut(s) 3210
BsoBI CYCGRG 3 cut(s) 1613, 2111, 2283
Bsp119I TTCGAA 1 cut(s) 2208
Bsp1286I GDGCHC 4 cut(s) 1262, 1584, 1875, 3039
Bsp1720I GCTNAGC 2 cut(s) 1578, 2704
BspANI GGCC 9 cut(s) 113, 147, 228, 281, 595, 1009, 1126, 1503, 3413
BspCNI CTCAG 5 cut(s) 832, 1570, 2135, 3108, 3373
BspFNI CGCG 4 cut(s) 935, 1712, 2935, 2947
BspHI TCATGA 3 cut(s) 1468, 2131, 2137
BspLI GGNNCC 5 cut(s) 241, 1261, 1822, 2291, 2502
BspMAI CTGCAG 7 cut(s) 101, 640, 1492, 1576, 2025, 2632, 3404
BspMI ACCTGC 3 cut(s) 90, 1441, 1489
BspOI GCTAGC 1 cut(s) 3503
BspPI GGATC 8 cut(s) 234, 247, 838, 1160, 1286, 2136, 2437, 3148
BspQI GCTCTTC 1 cut(s) 1212
BspT104I TTCGAA 1 cut(s) 2208
BspT107I GGYRCC 2 cut(s) 1259, 1820
BspTI CTTAAG 1 cut(s) 2189
BspTNI GGTCTC 1 cut(s) 3210
BsrDI GCAATG 5 cut(s) 608, 1182, 1411, 2402, 3139
BsrFI RCCGGY 1 cut(s) 113
BsrI ACTGG 6 cut(s) 566, 1142, 2008, 2492, 2897, 3040
BssAI RCCGGY 1 cut(s) 113
BssECI CCNNGG 4 cut(s) 235, 1498, 3266, 3414
BssHII GCGCGC 1 cut(s) 2933
BssSI CACGAG 1 cut(s) 1287
BssT1I CCWWGG 1 cut(s) 235
Bst2BI CACGAG 1 cut(s) 1287
Bst2UI CCWGG 4 cut(s) 260, 1011, 2563, 3267
Bst4CI ACNGT 8 cut(s) 80, 301, 462, 469, 922, 2318, 2693, 3034
Bst6I CTCTTC 6 cut(s) 39, 1212, 1361, 2782, 3213, 3324
BstAFI CTTAAG 1 cut(s) 2189
BstAPI GCANNNNNTGC 1 cut(s) 2063
BstBAI YACGTR 1 cut(s) 1876
BstBI TTCGAA 1 cut(s) 2208
BstC8I GCNNGC 9 cut(s) 97, 115, 1128, 1173, 2393, 2571, 2935, 3200, 3501
BstF5I GGATG 5 cut(s) 583, 1016, 1127, 1612, 1843
BstFNI CGCG 4 cut(s) 935, 1712, 2935, 2947
BstH2I RGCGCY 1 cut(s) 442
BstHHI GCGC 3 cut(s) 441, 2935, 2937
BstMAI GTCTC 3 cut(s) 722, 1917, 3210
BstNI CCWGG 4 cut(s) 260, 1011, 2563, 3267
BstNSI RCATGY 3 cut(s) 1739, 2093, 2681
BstSCI CCNGG 6 cut(s) 258, 1009, 1130, 1498, 2561, 3265
BstSLI GKGCMC 3 cut(s) 1262, 1875, 3039
BstUI CGCG 4 cut(s) 935, 1712, 2935, 2947
BstV2I GAAGAC 1 cut(s) 381
BstX2I RGATCY 3 cut(s) 239, 843, 1705
BstXI CCANNNNNNTGG 1 cut(s) 603
BstYI RGATCY 3 cut(s) 239, 843, 1705
BsuRI GGCC 9 cut(s) 113, 147, 228, 281, 595, 1009, 1126, 1503, 3413
BtrI CACGTC 1 cut(s) 349
BtsCI GGATG 5 cut(s) 583, 1016, 1127, 1612, 1843
BtsI GCAGTG 2 cut(s) 1206, 1569
BveI ACCTGC 3 cut(s) 90, 1441, 1489
Cac8I GCNNGC 9 cut(s) 97, 115, 1128, 1173, 2393, 2571, 2935, 3200, 3501
CciI TCATGA 3 cut(s) 1468, 2131, 2137
CfoI GCGC 3 cut(s) 441, 2935, 2937
Cfr10I RCCGGY 1 cut(s) 113
Cfr13I GGNCC 5 cut(s) 280, 1013, 1646, 2660, 3526
Csp6I GTAC 9 cut(s) 656, 1033, 1135, 1369, 1493, 1618, 1821, 2299, 2577
CviQI GTAC 9 cut(s) 656, 1033, 1135, 1369, 1493, 1618, 1821, 2299, 2577
DraI TTTAAA 2 cut(s) 1102, 1186
DraIII CACNNNGTG 2 cut(s) 1289, 2693
EaeI YGGCCR 2 cut(s) 111, 593
Eam1104I CTCTTC 6 cut(s) 39, 1212, 1361, 2782, 3213, 3324
EarI CTCTTC 6 cut(s) 39, 1212, 1361, 2782, 3213, 3324
Eco130I CCWWGG 1 cut(s) 235
Eco147I AGGCCT 2 cut(s) 228, 3413
Eco31I GGTCTC 1 cut(s) 3210
Eco32I GATATC 1 cut(s) 2623
Eco47I GGWCC 4 cut(s) 1013, 1646, 2660, 3526
Eco88I CYCGRG 3 cut(s) 1613, 2111, 2283
EcoRII CCWGG 4 cut(s) 258, 1009, 2561, 3265
EcoRV GATATC 1 cut(s) 2623
EcoT14I CCWWGG 1 cut(s) 235
EcoT22I ATGCAT 2 cut(s) 1411, 2091
ErhI CCWWGG 1 cut(s) 235
Esp3I CGTCTC 1 cut(s) 1917
FalI AAGNNNNNCTT 6 cut(s) 968, 1000, 2148, 2180, 3332, 3364
FaqI GGGAC 2 cut(s) 1825, 2263
FauI CCCGC 2 cut(s) 928, 3191
FbaI TGATCA 3 cut(s) 2134, 2382, 2514
FokI GGATG 5 cut(s) 570, 1003, 1134, 1619, 1830
FspBI CTAG 5 cut(s) 536, 711, 968, 2600, 3500
GlaI GCGC 3 cut(s) 440, 2934, 2936
GsaI CCCAGC 2 cut(s) 1049, 1466
HaeII RGCGCY 1 cut(s) 442
HaeIII GGCC 9 cut(s) 113, 147, 228, 281, 595, 1009, 1126, 1503, 3413
HapII CCGG 8 cut(s) 114, 217, 308, 316, 734, 1131, 1500, 2988
HhaI GCGC 3 cut(s) 441, 2935, 2937
Hin6I GCGC 3 cut(s) 439, 2933, 2935
HinP1I GCGC 3 cut(s) 439, 2933, 2935
HincII GTYRAC 1 cut(s) 773
HindII GTYRAC 1 cut(s) 773
HindIII AAGCTT 3 cut(s) 630, 1169, 2792
HpaII CCGG 8 cut(s) 114, 217, 308, 316, 734, 1131, 1500, 2988
HphI GGTGA 4 cut(s) 41, 436, 748, 2212
Hpy99I CGWCG 2 cut(s) 353, 2500
HpyCH4III ACNGT 8 cut(s) 80, 301, 462, 469, 922, 2318, 2693, 3034
HpyCH4IV ACGT 5 cut(s) 348, 775, 1875, 1926, 2908
HpySE526I ACGT 5 cut(s) 348, 775, 1875, 1926, 2908
HspAI GCGC 3 cut(s) 439, 2933, 2935
KpnI GGTACC 1 cut(s) 1824
KroI GCCGGC 1 cut(s) 113
KroNI GCCGGC 1 cut(s) 115
Ksp22I TGATCA 3 cut(s) 2134, 2382, 2514
LguI GCTCTTC 1 cut(s) 1212
LmnI GCTCC 4 cut(s) 445, 2161, 2182, 2500
LweI GCATC 8 cut(s) 238, 546, 592, 936, 1418, 1507, 2634, 2948
MaeI CTAG 5 cut(s) 536, 711, 968, 2600, 3500
MaeII ACGT 5 cut(s) 348, 775, 1875, 1926, 2908
MaeIII GTNAC 9 cut(s) 424, 855, 1111, 1422, 1476, 2092, 2483, 2948, 3214
MflI RGATCY 3 cut(s) 239, 843, 1705
MhlI GDGCHC 4 cut(s) 1262, 1584, 1875, 3039
MlsI TGGCCA 1 cut(s) 595
MluNI TGGCCA 1 cut(s) 595
MlyI GAGTC 6 cut(s) 513, 724, 1847, 2385, 3211, 3341
MmeI TCCRAC 5 cut(s) 331, 1846, 2519, 2590, 2677
Mox20I TGGCCA 1 cut(s) 595
Mph1103I ATGCAT 2 cut(s) 1411, 2091
MroNI GCCGGC 1 cut(s) 113
MroXI GAANNNNTTC 1 cut(s) 2106
MscI TGGCCA 1 cut(s) 595
MslI CAYNNNNRTG 3 cut(s) 2136, 2142, 2739
Msp20I TGGCCA 1 cut(s) 595
MspA1I CMGCKG 5 cut(s) 641, 906, 1382, 1487, 1577
MspCI CTTAAG 1 cut(s) 2189
MspI CCGG 8 cut(s) 114, 217, 308, 316, 734, 1131, 1500, 2988
MspR9I CCNGG 6 cut(s) 260, 1011, 1132, 1500, 2563, 3267
Mva1269I GAATGC 2 cut(s) 547, 3307
MvaI CCWGG 4 cut(s) 260, 1011, 2563, 3267
MvnI CGCG 4 cut(s) 935, 1712, 2935, 2947
NaeI GCCGGC 1 cut(s) 115
NciI CCSGG 2 cut(s) 1132, 1500
NgoMIV GCCGGC 1 cut(s) 113
NheI GCTAGC 1 cut(s) 3499
NlaIV GGNNCC 5 cut(s) 241, 1261, 1822, 2291, 2502
NmuCI GTSAC 4 cut(s) 424, 855, 1111, 3214
NsiI ATGCAT 2 cut(s) 1411, 2091
NspI RCATGY 3 cut(s) 1739, 2093, 2681
NspV TTCGAA 1 cut(s) 2208
PaeR7I CTCGAG 2 cut(s) 1613, 2283
PagI TCATGA 3 cut(s) 1468, 2131, 2137
PauI GCGCGC 1 cut(s) 2933
PceI AGGCCT 2 cut(s) 228, 3413
PciI ACATGT 1 cut(s) 1735
PciSI GCTCTTC 1 cut(s) 1212
PctI GAATGC 2 cut(s) 547, 3307
PdiI GCCGGC 1 cut(s) 115
PdmI GAANNNNTTC 1 cut(s) 2106
PflMI CCANNNNNTGG 1 cut(s) 1201
PleI GAGTC 6 cut(s) 513, 724, 1846, 2384, 3211, 3340
PpsI GAGTC 6 cut(s) 513, 724, 1846, 2384, 3211, 3340
Ppu21I YACGTR 1 cut(s) 1876
PscI ACATGT 1 cut(s) 1735
PshBI ATTAAT 1 cut(s) 2637
Psp6I CCWGG 4 cut(s) 258, 1009, 2561, 3265
PspFI CCCAGC 2 cut(s) 1045, 1462
PspGI CCWGG 4 cut(s) 258, 1009, 2561, 3265
PspN4I GGNNCC 5 cut(s) 241, 1261, 1822, 2291, 2502
PspPI GGNCC 5 cut(s) 280, 1013, 1646, 2660, 3526
PspXI VCTCGAGB 1 cut(s) 2283
PsrI GAACNNNNNNTAC 2 cut(s) 2282, 2314
PstI CTGCAG 7 cut(s) 101, 640, 1492, 1576, 2025, 2632, 3404
PsuI RGATCY 3 cut(s) 239, 843, 1705
PteI GCGCGC 1 cut(s) 2933
PvuII CAGCTG 4 cut(s) 641, 906, 1382, 1577
RsaI GTAC 9 cut(s) 657, 1034, 1136, 1370, 1494, 1619, 1822, 2300, 2578
RsaNI GTAC 9 cut(s) 656, 1033, 1135, 1369, 1493, 1618, 1821, 2299, 2577
RseI CAYNNNNRTG 3 cut(s) 2136, 2142, 2739
SapI GCTCTTC 1 cut(s) 1212
Sau96I GGNCC 5 cut(s) 280, 1013, 1646, 2660, 3526
SbfI CCTGCAGG 1 cut(s) 101
ScaI AGTACT 1 cut(s) 2300
SchI GAGTC 6 cut(s) 513, 724, 1847, 2385, 3211, 3341
ScrFI CCNGG 6 cut(s) 260, 1011, 1132, 1500, 2563, 3267
SdaI CCTGCAGG 1 cut(s) 101
SduI GDGCHC 4 cut(s) 1262, 1584, 1875, 3039
SfaNI GCATC 8 cut(s) 238, 546, 592, 936, 1418, 1507, 2634, 2948
Sfr274I CTCGAG 2 cut(s) 1613, 2283
SfuI TTCGAA 1 cut(s) 2208
SinI GGWCC 4 cut(s) 1013, 1646, 2660, 3526
SlaI CTCGAG 2 cut(s) 1613, 2283
SmiMI CAYNNNNRTG 3 cut(s) 2136, 2142, 2739
SmlI CTYRAG 5 cut(s) 137, 1613, 2189, 2257, 2283
SmoI CTYRAG 5 cut(s) 137, 1613, 2189, 2257, 2283
SpeI ACTAGT 2 cut(s) 535, 710
Sse8387I CCTGCAGG 1 cut(s) 101
SseBI AGGCCT 2 cut(s) 228, 3413
SspI AATATT 2 cut(s) 1828, 2330
SspMI CTAG 5 cut(s) 536, 711, 968, 2600, 3500
StuI AGGCCT 2 cut(s) 228, 3413
StyD4I CCNGG 6 cut(s) 258, 1009, 1130, 1498, 2561, 3265
StyI CCWWGG 1 cut(s) 235
TaaI ACNGT 8 cut(s) 80, 301, 462, 469, 922, 2318, 2693, 3034
TaiI ACGT 5 cut(s) 351, 778, 1878, 1929, 2911
TaqI TCGA 8 cut(s) 107, 203, 571, 1364, 1614, 1635, 2208, 2284
TaqII GACCGA 1 cut(s) 2677
TatI WGTACW 4 cut(s) 655, 1492, 1617, 2298
TauI GCSGC 3 cut(s) 120, 150, 1487
TseFI GTSAC 4 cut(s) 424, 855, 1111, 3214
Tsp45I GTSAC 4 cut(s) 424, 855, 1111, 3214
TspGWI ACGGA 4 cut(s) 927, 1410, 2195, 2513
Van91I CCANNNNNTGG 1 cut(s) 1201
Vha464I CTTAAG 1 cut(s) 2189
VneI GTGCAC 1 cut(s) 3035
VpaK11BI GGWCC 4 cut(s) 1013, 1646, 2660, 3526
VspI ATTAAT 1 cut(s) 2637
XapI RAATTY 4 cut(s) 751, 1930, 2420, 3461
XceI RCATGY 3 cut(s) 1739, 2093, 2681
XcmI CCANNNNNNNNNTGG 1 cut(s) 266
XhoI CTCGAG 2 cut(s) 1613, 2283
XmnI GAANNNNTTC 1 cut(s) 2106
XspI CTAG 5 cut(s) 536, 711, 968, 2600, 3500
ZrmI AGTACT 1 cut(s) 2300
Zsp2I ATGCAT 2 cut(s) 1411, 2091
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.