FvH4_6g20760

Protein ROOT PRIMORDIUM DEFECTIVE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0009141
Physical Location & Seq
Forward (+)
14301277 .. 14303574
2298 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g20760.t1

Sequence Viewer

Length: 1281 bp
ATGTTTGGGAAGAGATACCGGTCCTCCGCGGAGTGGTGGTCAAAGAATCAGAACCGGTTCATGACCACCAGTAAGCGTGTGCAGGACAGGAGCAAAATGAAGAGGGTTCATGATCTTGAGGTTGTGACTGAGAAATGGAAGATAGCATCCAAGGTGTTGTTCTTGATGGAGTTACTGAAGAAGGAGCTGGAAATGATAATGCCGGTGAGGTCATTGGACAAGTTCCGTGCGCAGATTAGTCTTCCTAAGCCGCATAGGATGTCTGATTTCATTAGGAAATCTCCGAAACTGTTTGAGTTGTATAAGGACCGTAGGGGAGTTTTGTGGTGTGGGATGACTGAGCAAGCTGAGGAGTTGGTTGAGGAAGAGGATAGGATACTTGAGGAGCATCAAGATAAGGCTGCTGAGTATGTTACGCGATTTCTTATGATGTCTGTTGATAAGCGGCTTCGAGTTGACAAGATTGCTCATTACAGGAGAGACTTCGCTCTTCCTAATGATTTTAGGACTACCTGGGTGCATAAGTATCCACAATACTTTAGACTGGTCCGGGATAATGAAGACATTGAATACTTGGAACTTGTTTCTTGGAATCCTGCTTGGGCTATAACGGAGTTGGAGAAGAAGACAATGGGGGTTACTGATGCCAGTGCTCATACCCCTGGATTGCTGACACTCCCGTTTCCTTTGAAGTTCCCTCCCAATTACAAGAAGAGAGTGTACAAGTATCGGGGAGCACTTGATCATTTTCAGAAAAGGTCTTATTTGTCACCTTATGCAGATGCCAGGGGACTCAAAGCCGGGTCGAAAGAATTTGACAAGAGGGCAGTGGCTATTATGCATGAGCTTCTTAGCTTTACTGTAGAGAAGAGATTGGTGACTGATCACCTCACTCACTTCAGACAGGAACTTGTGATGCCCCAAAAGCTTATGAGACTATTTCTGAAGCATTTTGGCATTTTTTATGTCTCGGAGAGGGGGAAGAGATTTAGTGTCTTCTTGACAGAAGCTTATGAAGGTTCAGAGCTCATTGAGAAGTGCCCTTTGGTTATTTGGAAGGAAAAAGTTCAGAGCCTTATTGGTTACAGGAAAAAGAAAAAGATCGAGACTTTTACTGACATGTCAGACATGGAGGACAGTAGTTTATATAGGAGCAGTTCTGAAAGTGAAAGTGATTCTGAAGAAGAGAGCATTCACTTGGAGGATAACTCACATACAGATAATTCTGAAATGGAGATTGACGAGGTTTTCAGAGCTTACGAGGACTCTAAAACATCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PORR

Protein Analysis

427

Amino Acids

50.75

Weight (kDa)

8.89

Isoelectric Point (pI)

53.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PORR PF11955 34 - 364 1e-106 Plant organelle RNA recognition domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 231
AccII CGCG 2 cut(s) 29, 418
AciI CCGC 4 cut(s) 27, 29, 251, 445
AcsI RAATTY 1 cut(s) 812
AcuI CTGAAG 4 cut(s) 197, 883, 965, 1200
AfaI GTAC 1 cut(s) 722
AfiI CCNNNNNNNGG 1 cut(s) 33
AflIII ACRYGT 1 cut(s) 1119
AgeI ACCGGT 2 cut(s) 18, 54
AgsI TTSAA 2 cut(s) 569, 691
AjnI CCWGG 3 cut(s) 512, 661, 785
AjuI GAANNNNNNNTTGG 4 cut(s) 143, 175, 1028, 1060
AluBI AGCT 8 cut(s) 187, 347, 847, 855, 928, 1010, 1027, 1256
AluI AGCT 8 cut(s) 187, 347, 847, 855, 928, 1010, 1027, 1256
Alw21I GWGCWC 3 cut(s) 655, 739, 1029
Alw26I GTCTC 4 cut(s) 474, 928, 973, 1100
ApeKI GCWGC 1 cut(s) 401
ApoI RAATTY 1 cut(s) 812
AsiGI ACCGGT 2 cut(s) 18, 54
Asp700I GAANNNNTTC 2 cut(s) 56, 1065
AspLEI GCGC 1 cut(s) 232
AspS9I GGNCC 3 cut(s) 21, 307, 547
AsuC2I CCSGG 2 cut(s) 551, 802
AsuHPI GGTGA 4 cut(s) 217, 762, 878, 889
AvaII GGWCC 3 cut(s) 21, 307, 547
BaeGI GKGCMC 1 cut(s) 1043
BanII GRGCYC 1 cut(s) 1029
BarI GAAGNNNNNNTAC 2 cut(s) 704, 736
BbsI GAAGAC 4 cut(s) 233, 567, 632, 988
Bbv12I GWGCWC 3 cut(s) 655, 739, 1029
BbvCI CCTCAGC 1 cut(s) 348
BbvI GCAGC 1 cut(s) 388
BccI CCATC 1 cut(s) 160
BciT130I CCWGG 3 cut(s) 514, 663, 787
BciVI GTATCC 2 cut(s) 369, 537
BclI TGATCA 2 cut(s) 742, 883
BcnI CCSGG 2 cut(s) 551, 802
BcoDI GTCTC 4 cut(s) 474, 928, 973, 1100
BfmI CTRYAG 1 cut(s) 861
BfuI GTATCC 2 cut(s) 369, 537
BisI GCNGC 3 cut(s) 251, 402, 446
BlsI GCNGC 3 cut(s) 252, 403, 447
Bme1390I CCNGG 5 cut(s) 514, 551, 663, 787, 802
Bme18I GGWCC 3 cut(s) 21, 307, 547
BmgT120I GGNCC 3 cut(s) 21, 307, 547
BmrFI CCNGG 5 cut(s) 514, 551, 663, 787, 802
BmsI GCATC 5 cut(s) 155, 397, 634, 772, 906
BpiI GAAGAC 4 cut(s) 233, 567, 632, 988
BplI GAGNNNNNCTC 2 cut(s) 1193, 1225
Bpu10I CCTNAGC 2 cut(s) 246, 348
BpuEI CTTGAG 2 cut(s) 137, 401
BpuMI CCSGG 2 cut(s) 551, 802
BsaJI CCNNGG 5 cut(s) 27, 150, 513, 661, 786
BsaWI WCCGGW 2 cut(s) 18, 54
BsaXI ACNNNNNCTCC 4 cut(s) 8, 23, 38, 53
Bsc4I CCNNNNNNNGG 1 cut(s) 33
Bse118I RCCGGY 3 cut(s) 18, 54, 202
Bse1I ACTGG 3 cut(s) 69, 549, 648
BseBI CCWGG 3 cut(s) 514, 663, 787
BseDI CCNNGG 5 cut(s) 27, 150, 513, 661, 786
BseGI GGATG 3 cut(s) 146, 264, 339
BseLI CCNNNNNNNGG 1 cut(s) 33
BseMII CTCAG 4 cut(s) 120, 330, 339, 396
BseNI ACTGG 3 cut(s) 69, 549, 648
BseRI GAGGAG 2 cut(s) 365, 398
BseSI GKGCMC 1 cut(s) 1043
BseXI GCAGC 1 cut(s) 388
BsgI GTGCAG 1 cut(s) 101
Bsh1236I CGCG 2 cut(s) 29, 418
BshTI ACCGGT 2 cut(s) 18, 54
BsiHKAI GWGCWC 3 cut(s) 655, 739, 1029
BsiSI CCGG 5 cut(s) 19, 55, 203, 550, 801
BslFI GGGAC 1 cut(s) 804
BslI CCNNNNNNNGG 1 cut(s) 33
BsmAI GTCTC 4 cut(s) 474, 928, 973, 1100
BsmFI GGGAC 1 cut(s) 804
BsmI GAATGC 1 cut(s) 1191
Bsp1286I GDGCHC 4 cut(s) 655, 739, 1029, 1043
Bsp1407I TGTACA 1 cut(s) 720
Bsp143I GATC 4 cut(s) 112, 742, 883, 1101
BspACI CCGC 4 cut(s) 27, 29, 251, 445
BspCNI CTCAG 4 cut(s) 121, 331, 340, 397
BspFNI CGCG 2 cut(s) 29, 418
BspHI TCATGA 2 cut(s) 60, 109
BspQI GCTCTTC 1 cut(s) 495
BsrFI RCCGGY 3 cut(s) 18, 54, 202
BsrGI TGTACA 1 cut(s) 720
BsrI ACTGG 3 cut(s) 69, 549, 648
BssAI RCCGGY 3 cut(s) 18, 54, 202
BssECI CCNNGG 5 cut(s) 27, 150, 513, 661, 786
BssMI GATC 4 cut(s) 112, 742, 883, 1101
BssT1I CCWWGG 1 cut(s) 150
Bst2UI CCWGG 3 cut(s) 514, 663, 787
Bst4CI ACNGT 4 cut(s) 291, 311, 862, 1139
Bst6I CTCTTC 8 cut(s) 5, 95, 360, 495, 707, 863, 977, 1179
BstAUI TGTACA 1 cut(s) 720
BstC8I GCNNGC 1 cut(s) 345
BstDEI CTNAG 7 cut(s) 129, 246, 339, 348, 405, 851, 1278
BstDSI CCRYGG 1 cut(s) 27
BstF5I GGATG 3 cut(s) 146, 264, 339
BstFNI CGCG 2 cut(s) 29, 418
BstHHI GCGC 1 cut(s) 232
BstKTI GATC 4 cut(s) 115, 745, 886, 1104
BstMAI GTCTC 4 cut(s) 474, 928, 973, 1100
BstMBI GATC 4 cut(s) 112, 742, 883, 1101
BstMWI GCNNNNNNNGC 1 cut(s) 925
BstNI CCWGG 3 cut(s) 514, 663, 787
BstNSI RCATGY 1 cut(s) 1123
BstSCI CCNGG 5 cut(s) 512, 549, 661, 785, 800
BstSFI CTRYAG 1 cut(s) 861
BstSLI GKGCMC 1 cut(s) 1043
BstUI CGCG 2 cut(s) 29, 418
BstV1I GCAGC 1 cut(s) 388
BstV2I GAAGAC 4 cut(s) 233, 567, 632, 988
BsuI GTATCC 2 cut(s) 369, 537
BtgI CCRYGG 1 cut(s) 27
BtsCI GGATG 3 cut(s) 146, 264, 339
BtsI GCAGTG 1 cut(s) 834
BtsIMutI CAGTG 2 cut(s) 655, 834
Cac8I GCNNGC 1 cut(s) 345
CciI TCATGA 2 cut(s) 60, 109
CfoI GCGC 1 cut(s) 232
Cfr10I RCCGGY 3 cut(s) 18, 54, 202
Cfr13I GGNCC 3 cut(s) 21, 307, 547
Cfr42I CCGCGG 1 cut(s) 30
Csp6I GTAC 1 cut(s) 721
CspAI ACCGGT 2 cut(s) 18, 54
CviAII CATG 5 cut(s) 61, 110, 842, 1120, 1129
CviQI GTAC 1 cut(s) 721
DdeI CTNAG 7 cut(s) 129, 246, 339, 348, 405, 851, 1278
DpnI GATC 4 cut(s) 114, 744, 885, 1103
DpnII GATC 4 cut(s) 112, 742, 883, 1101
Eam1104I CTCTTC 8 cut(s) 5, 95, 360, 495, 707, 863, 977, 1179
EarI CTCTTC 8 cut(s) 5, 95, 360, 495, 707, 863, 977, 1179
Ecl136II GAGCTC 1 cut(s) 1027
Eco130I CCWWGG 1 cut(s) 150
Eco24I GRGCYC 1 cut(s) 1029
Eco47I GGWCC 3 cut(s) 21, 307, 547
Eco53kI GAGCTC 1 cut(s) 1027
Eco57I CTGAAG 4 cut(s) 197, 883, 965, 1200
EcoICRI GAGCTC 1 cut(s) 1027
EcoRII CCWGG 3 cut(s) 512, 661, 785
EcoT14I CCWWGG 1 cut(s) 150
EcoT22I ATGCAT 1 cut(s) 843
EcoT38I GRGCYC 1 cut(s) 1029
ErhI CCWWGG 1 cut(s) 150
FaeI CATG 5 cut(s) 64, 113, 845, 1123, 1132
FaqI GGGAC 1 cut(s) 804
FatI CATG 5 cut(s) 60, 109, 841, 1119, 1128
FbaI TGATCA 2 cut(s) 742, 883
Fnu4HI GCNGC 3 cut(s) 251, 402, 446
FokI GGATG 3 cut(s) 133, 271, 346
FriOI GRGCYC 1 cut(s) 1029
Fsp4HI GCNGC 3 cut(s) 251, 402, 446
FspI TGCGCA 1 cut(s) 231
GlaI GCGC 1 cut(s) 231
GluI GCNGC 3 cut(s) 251, 402, 446
HapII CCGG 5 cut(s) 19, 55, 203, 550, 801
HhaI GCGC 1 cut(s) 232
Hin1II CATG 5 cut(s) 64, 113, 845, 1123, 1132
Hin6I GCGC 1 cut(s) 230
HinP1I GCGC 1 cut(s) 230
HincII GTYRAC 1 cut(s) 457
HindII GTYRAC 1 cut(s) 457
HindIII AAGCTT 2 cut(s) 926, 1008
HinfI GANTC 5 cut(s) 46, 592, 792, 1175, 1265
HpaII CCGG 5 cut(s) 19, 55, 203, 550, 801
HphI GGTGA 4 cut(s) 217, 762, 878, 889
Hpy166II GTNNAC 2 cut(s) 457, 721
Hpy188III TCNNGA 7 cut(s) 61, 110, 116, 163, 392, 1000, 1105
Hpy8I GTNNAC 2 cut(s) 457, 721
HpyAV CCTTC 3 cut(s) 175, 1010, 1051
HpyCH4III ACNGT 4 cut(s) 291, 311, 862, 1139
HpyCH4V TGCA 4 cut(s) 82, 520, 779, 841
HpyF10VI GCNNNNNNNGC 1 cut(s) 925
HpyF3I CTNAG 7 cut(s) 129, 246, 339, 348, 405, 851, 1278
Hsp92II CATG 5 cut(s) 64, 113, 845, 1123, 1132
HspAI GCGC 1 cut(s) 230
Ksp22I TGATCA 2 cut(s) 742, 883
KspI CCGCGG 1 cut(s) 30
Kzo9I GATC 4 cut(s) 112, 742, 883, 1101
LguI GCTCTTC 1 cut(s) 495
LmnI GCTCC 5 cut(s) 90, 184, 385, 734, 1152
Lsp1109I GCAGC 1 cut(s) 388
LweI GCATC 5 cut(s) 155, 397, 634, 772, 906
MaeIII GTNAC 7 cut(s) 124, 171, 412, 637, 768, 877, 1082
MalI GATC 4 cut(s) 114, 744, 885, 1103
MboI GATC 4 cut(s) 112, 742, 883, 1101
MhlI GDGCHC 4 cut(s) 655, 739, 1029, 1043
MluCI AATT 3 cut(s) 703, 812, 1222
MlyI GAGTC 2 cut(s) 786, 1259
MmeI TCCRAC 1 cut(s) 597
Mph1103I ATGCAT 1 cut(s) 843
MroXI GAANNNNTTC 2 cut(s) 56, 1065
MspA1I CMGCKG 1 cut(s) 29
MspI CCGG 5 cut(s) 19, 55, 203, 550, 801
MspR9I CCNGG 5 cut(s) 514, 551, 663, 787, 802
Mva1269I GAATGC 1 cut(s) 1191
MvaI CCWGG 3 cut(s) 514, 663, 787
MvnI CGCG 2 cut(s) 29, 418
MwoI GCNNNNNNNGC 1 cut(s) 925
NciI CCSGG 2 cut(s) 551, 802
NdeII GATC 4 cut(s) 112, 742, 883, 1101
NlaIII CATG 5 cut(s) 64, 113, 845, 1123, 1132
NmuCI GTSAC 3 cut(s) 124, 768, 877
NsbI TGCGCA 1 cut(s) 231
NsiI ATGCAT 1 cut(s) 843
NspI RCATGY 1 cut(s) 1123
PagI TCATGA 2 cut(s) 60, 109
PciI ACATGT 1 cut(s) 1119
PciSI GCTCTTC 1 cut(s) 495
PctI GAATGC 1 cut(s) 1191
PdmI GAANNNNTTC 2 cut(s) 56, 1065
PfeI GAWTC 3 cut(s) 46, 592, 1175
PfoI TCCNGGA 1 cut(s) 549
PinAI ACCGGT 2 cut(s) 18, 54
PkrI GCNGC 3 cut(s) 252, 403, 447
PleI GAGTC 2 cut(s) 786, 1259
PpsI GAGTC 2 cut(s) 786, 1259
PscI ACATGT 1 cut(s) 1119
Psp124BI GAGCTC 1 cut(s) 1029
Psp6I CCWGG 3 cut(s) 512, 661, 785
PspGI CCWGG 3 cut(s) 512, 661, 785
PspPI GGNCC 3 cut(s) 21, 307, 547
RsaI GTAC 1 cut(s) 722
RsaNI GTAC 1 cut(s) 721
SacI GAGCTC 1 cut(s) 1029
SacII CCGCGG 1 cut(s) 30
SapI GCTCTTC 1 cut(s) 495
SatI GCNGC 3 cut(s) 251, 402, 446
Sau3AI GATC 4 cut(s) 112, 742, 883, 1101
Sau96I GGNCC 3 cut(s) 21, 307, 547
SchI GAGTC 2 cut(s) 786, 1259
ScrFI CCNGG 5 cut(s) 514, 551, 663, 787, 802
SduI GDGCHC 4 cut(s) 655, 739, 1029, 1043
SfaNI GCATC 5 cut(s) 155, 397, 634, 772, 906
SfcI CTRYAG 1 cut(s) 861
Sfr303I CCGCGG 1 cut(s) 30
SgrBI CCGCGG 1 cut(s) 30
SinI GGWCC 3 cut(s) 21, 307, 547
SmlI CTYRAG 2 cut(s) 116, 380
SmoI CTYRAG 2 cut(s) 116, 380
Sse9I AATT 3 cut(s) 703, 812, 1222
SsiI CCGC 4 cut(s) 27, 29, 251, 445
SstI GAGCTC 1 cut(s) 1029
StyD4I CCNGG 5 cut(s) 512, 549, 661, 785, 800
StyI CCWWGG 1 cut(s) 150
TaaI ACNGT 4 cut(s) 291, 311, 862, 1139
TaqI TCGA 3 cut(s) 451, 806, 1104
TasI AATT 3 cut(s) 703, 812, 1222
TatI WGTACW 1 cut(s) 720
TauI GCSGC 2 cut(s) 253, 448
TfiI GAWTC 3 cut(s) 46, 592, 1175
TscAI CASTG 2 cut(s) 655, 834
TseFI GTSAC 3 cut(s) 124, 768, 877
TseI GCWGC 1 cut(s) 401
Tsp45I GTSAC 3 cut(s) 124, 768, 877
TspDTI ATGAA 6 cut(s) 49, 98, 113, 259, 573, 1029
TspGWI ACGGA 2 cut(s) 215, 626
TspRI CASTG 2 cut(s) 655, 834
VpaK11BI GGWCC 3 cut(s) 21, 307, 547
XapI RAATTY 1 cut(s) 812
XceI RCATGY 1 cut(s) 1123
XmnI GAANNNNTTC 2 cut(s) 56, 1065
Zsp2I ATGCAT 1 cut(s) 843
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.