MD14G1152200.v1.1

Protein ROOT PRIMORDIUM DEFECTIVE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0009141
Physical Location & Seq
Reverse (-)
24641286 .. 24642581
1296 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1152200.v1.1.491

Sequence Viewer

Length: 1296 bp
ATGTTTGTGAGACGATACTGGTCCGGTGAGTGGTGGTCAATGAACCAGAAGCGGTTCATGACCACCAGCAAGAGGGTGCAAGACAGAAGCAAATTGAAGAGGATTCATGATCTCGAGATTGTGACTGAGAAATGGAAGATAGCATCCAAAGTGTTGTTCTTGATGGAAATTCTGAAAAAAGAGCCCGAAATGATCATTCCCGTCAGGCAATTGGATCAGTACCGTAGCCAGATTAGTCTCCCGAAGCCCCGCAGGGTGTCTGATTTTATTCGAAAATCGCCGAAACTGTTTGAATTGTACAAGGATCACAGAGGGATCTTATGGTGTGGGATGACCAAGCAAGGTGAGGAATTGGTACAAGAGGAGAACAGAATGCTTGAGGAGCATCAAGATACCGCGGCCGAATTCGTGACACGCTTTCTTATGATGTCTGTCGATAAAAGGCTGGCATTGGACAAGATTGTCCATTTCAGGAGGGATTTTGCTCTGCCAGTTGATTTCAGGACTAATTGGGTTCACAAATATCCCCAATATTTCAAGGTGGTTAAAGATAATGAGGATGTTGAGCATTTGGAACTTGTTAATTGGAATCATGCGTGGGCTATAACCGAGCTGGAGAAGAAGACAATGGGGATCACTGAGGCCAGTGATGATCATGTCCCGGGCTTGCTTTCGCTCTCTTTTCCGTTGAAGTTCCCCCCAAATTATAAAAAGGCGTACAAGTACAAAGGAGCCATCGATCATTTTCAGAAAAGGTCTTATTTATCTCCATACGCGGATGCCAGGGGACTCAAAGCCGGCTCTAAAGAATTTGACAAGAGGGCAGTTGCTATTATGCATGAGTTGCTTAGTTTTACTGTGGAGAAGAGATTGGTTACTGATCACCTTACTCATTTTCGACGCGAACTTGTGATGCCACAGAAGCTTATGAGGCTTCTGCTGAAGCATTTTGGCATCTTCTATGTTTCTGAGAGGGGGAAGAGGTTTAGCGTGTTCTTGAAGGAAGCTTACAAAGGTTCGGAGCTGATTGAGAAATGCCCTTTGGTGGTTTGGAAGGAAAAAGTCCAGAGCCTTATCGGTTATAGGGGAAAGAAGAAAATTGAAGCTTTCAGTGACATGGAAGACATGGAAGAGAATGGTTTCTATGAGAGCAGTTCAGAAAGTGAGGACACCATTGACTTGCAGCTTGAGCAAGAGGAGAAGGTGGGTGAGTTGGAGGATGATTCACTTGCGGTTTATTCTGAAATGGACATTGAAGAGATTTGCAGGGCATATGAGGATACAAAAAGATGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PORR

Protein Analysis

432

Amino Acids

51.3

Weight (kDa)

8.36

Isoelectric Point (pI)

46.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PORR PF11955 33 - 361 7.2e-108 Plant organelle RNA recognition domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 708
AasI GACNNNNNNGTC 1 cut(s) 461
AccII CGCG 3 cut(s) 398, 776, 903
AciI CCGC 6 cut(s) 52, 250, 396, 398, 776, 1232
AclWI GGATC 4 cut(s) 222, 312, 323, 641
AcoI YGGCCR 1 cut(s) 399
AcsI RAATTY 3 cut(s) 168, 404, 809
AcuI CTGAAG 1 cut(s) 962
AfaI GTAC 5 cut(s) 221, 299, 357, 719, 725
AfiI CCNNNNNNNGG 3 cut(s) 30, 72, 1045
AgsI TTSAA 7 cut(s) 97, 293, 538, 691, 1000, 1103, 1256
AjnI CCWGG 1 cut(s) 782
AjuI GAANNNNNNNTTGG 4 cut(s) 140, 172, 1025, 1057
AluBI AGCT 6 cut(s) 613, 925, 1007, 1024, 1106, 1186
AluI AGCT 6 cut(s) 613, 925, 1007, 1024, 1106, 1186
Alw26I GTCTC 2 cut(s) 4, 242
AlwI GGATC 4 cut(s) 222, 312, 323, 641
Ama87I CYCGRG 2 cut(s) 113, 661
AoxI GGCC 2 cut(s) 399, 642
ApeKI GCWGC 1 cut(s) 1183
ApoI RAATTY 3 cut(s) 168, 404, 809
Asp700I GAANNNNTTC 2 cut(s) 53, 1139
AspS9I GGNCC 1 cut(s) 21
AsuC2I CCSGG 2 cut(s) 662, 663
AsuHPI GGTGA 4 cut(s) 38, 356, 875, 1220
AsuII TTCGAA 1 cut(s) 271
AvaI CYCGRG 2 cut(s) 113, 661
AvaII GGWCC 1 cut(s) 21
BanII GRGCYC 1 cut(s) 186
BarI GAAGNNNNNNTAC 2 cut(s) 992, 1024
BbsI GAAGAC 2 cut(s) 629, 1128
BbvI GCAGC 1 cut(s) 1195
BccI CCATC 2 cut(s) 157, 743
BciT130I CCWGG 1 cut(s) 784
BciVI GTATCC 1 cut(s) 1273
BclI TGATCA 3 cut(s) 192, 652, 880
BcnI CCSGG 2 cut(s) 662, 663
BcoDI GTCTC 2 cut(s) 4, 242
BfuI GTATCC 1 cut(s) 1273
BisI GCNGC 2 cut(s) 399, 1184
BlsI GCNGC 2 cut(s) 400, 1185
Bme1390I CCNGG 3 cut(s) 662, 663, 784
Bme18I GGWCC 1 cut(s) 21
BmeT110I CYCGRG 2 cut(s) 113, 661
BmgT120I GGNCC 1 cut(s) 21
BmiI GGNNCC 1 cut(s) 733
BmrFI CCNGG 3 cut(s) 662, 663, 784
BmsI GCATC 5 cut(s) 152, 394, 769, 903, 963
BpiI GAAGAC 2 cut(s) 629, 1128
BpmI CTGGAG 1 cut(s) 635
Bpu14I TTCGAA 1 cut(s) 271
BpuEI CTTGAG 2 cut(s) 398, 1208
BpuMI CCSGG 2 cut(s) 662, 663
Bsa29I ATCGAT 1 cut(s) 738
BsaJI CCNNGG 3 cut(s) 396, 661, 783
BsaWI WCCGGW 1 cut(s) 23
Bsc4I CCNNNNNNNGG 3 cut(s) 30, 72, 1045
Bse118I RCCGGY 1 cut(s) 797
Bse1I ACTGG 3 cut(s) 23, 491, 645
BseBI CCWGG 1 cut(s) 784
BseCI ATCGAT 1 cut(s) 738
BseDI CCNNGG 3 cut(s) 396, 661, 783
BseGI GGATG 5 cut(s) 143, 336, 565, 784, 1225
BseLI CCNNNNNNNGG 3 cut(s) 30, 72, 1045
BseMII CTCAG 3 cut(s) 117, 630, 960
BseNI ACTGG 3 cut(s) 23, 491, 645
BseRI GAGGAG 3 cut(s) 377, 395, 1211
BseX3I CGGCCG 1 cut(s) 399
BseXI GCAGC 1 cut(s) 1195
Bsh1236I CGCG 3 cut(s) 398, 776, 903
Bsh1285I CGRYCG 1 cut(s) 402
BshFI GGCC 2 cut(s) 401, 644
BshVI ATCGAT 1 cut(s) 738
BsiEI CGRYCG 1 cut(s) 402
BsiHKCI CYCGRG 2 cut(s) 113, 661
BsiSI CCGG 3 cut(s) 24, 662, 798
BslFI GGGAC 2 cut(s) 644, 801
BslI CCNNNNNNNGG 3 cut(s) 30, 72, 1045
BsmAI GTCTC 2 cut(s) 4, 242
BsmBI CGTCTC 1 cut(s) 4
BsmFI GGGAC 2 cut(s) 644, 801
BsmI GAATGC 1 cut(s) 378
BsnI GGCC 2 cut(s) 401, 644
BsoBI CYCGRG 2 cut(s) 113, 661
Bsp119I TTCGAA 1 cut(s) 271
Bsp1286I GDGCHC 1 cut(s) 186
Bsp1407I TGTACA 1 cut(s) 297
Bsp143I GATC 9 cut(s) 109, 192, 214, 304, 315, 633, 652, 739, 880
BspACI CCGC 6 cut(s) 52, 250, 396, 398, 776, 1232
BspANI GGCC 2 cut(s) 401, 644
BspCNI CTCAG 3 cut(s) 118, 631, 961
BspDI ATCGAT 1 cut(s) 738
BspFNI CGCG 3 cut(s) 398, 776, 903
BspHI TCATGA 2 cut(s) 57, 106
BspLI GGNNCC 1 cut(s) 733
BspPI GGATC 4 cut(s) 222, 312, 323, 641
BspT104I TTCGAA 1 cut(s) 271
BsrFI RCCGGY 1 cut(s) 797
BsrGI TGTACA 1 cut(s) 297
BsrI ACTGG 3 cut(s) 23, 491, 645
BssAI RCCGGY 1 cut(s) 797
BssECI CCNNGG 3 cut(s) 396, 661, 783
BssMI GATC 9 cut(s) 109, 192, 214, 304, 315, 633, 652, 739, 880
Bst2UI CCWGG 1 cut(s) 784
Bst4CI ACNGT 3 cut(s) 224, 288, 859
Bst6I CTCTTC 5 cut(s) 92, 860, 974, 1125, 1251
BstAPI GCANNNNNTGC 1 cut(s) 844
BstAUI TGTACA 1 cut(s) 297
BstBI TTCGAA 1 cut(s) 271
BstC8I GCNNGC 3 cut(s) 447, 668, 799
BstDEI CTNAG 4 cut(s) 126, 639, 848, 969
BstDSI CCRYGG 1 cut(s) 396
BstF5I GGATG 5 cut(s) 143, 336, 565, 784, 1225
BstFNI CGCG 3 cut(s) 398, 776, 903
BstKTI GATC 9 cut(s) 112, 195, 217, 307, 318, 636, 655, 742, 883
BstMAI GTCTC 2 cut(s) 4, 242
BstMBI GATC 9 cut(s) 109, 192, 214, 304, 315, 633, 652, 739, 880
BstMCI CGRYCG 1 cut(s) 402
BstMWI GCNNNNNNNGC 5 cut(s) 382, 844, 922, 931, 1189
BstNI CCWGG 1 cut(s) 784
BstSCI CCNGG 3 cut(s) 660, 661, 782
BstUI CGCG 3 cut(s) 398, 776, 903
BstV1I GCAGC 1 cut(s) 1195
BstV2I GAAGAC 2 cut(s) 629, 1128
BstX2I RGATCY 1 cut(s) 315
BstYI RGATCY 1 cut(s) 315
BstZI CGGCCG 1 cut(s) 399
Bsu15I ATCGAT 1 cut(s) 738
BsuI GTATCC 1 cut(s) 1273
BsuRI GGCC 2 cut(s) 401, 644
BsuTUI ATCGAT 1 cut(s) 738
BtgI CCRYGG 1 cut(s) 396
BtsCI GGATG 5 cut(s) 143, 336, 565, 784, 1225
BtsIMutI CAGTG 3 cut(s) 636, 652, 1117
Cac8I GCNNGC 3 cut(s) 447, 668, 799
CciI TCATGA 2 cut(s) 57, 106
Cfr10I RCCGGY 1 cut(s) 797
Cfr13I GGNCC 1 cut(s) 21
Cfr42I CCGCGG 1 cut(s) 399
Cfr9I CCCGGG 1 cut(s) 661
ClaI ATCGAT 1 cut(s) 738
CseI GACGC 1 cut(s) 909
Csp6I GTAC 5 cut(s) 220, 298, 356, 718, 724
CviAII CATG 7 cut(s) 58, 107, 593, 656, 839, 1117, 1126
CviQI GTAC 5 cut(s) 220, 298, 356, 718, 724
DdeI CTNAG 4 cut(s) 126, 639, 848, 969
DpnI GATC 9 cut(s) 111, 194, 216, 306, 317, 635, 654, 741, 882
DpnII GATC 9 cut(s) 109, 192, 214, 304, 315, 633, 652, 739, 880
DrdI GACNNNNNNGTC 1 cut(s) 461
DseDI GACNNNNNNGTC 1 cut(s) 461
EaeI YGGCCR 1 cut(s) 399
EagI CGGCCG 1 cut(s) 399
Eam1104I CTCTTC 5 cut(s) 92, 860, 974, 1125, 1251
EarI CTCTTC 5 cut(s) 92, 860, 974, 1125, 1251
EclXI CGGCCG 1 cut(s) 399
Eco24I GRGCYC 1 cut(s) 186
Eco47I GGWCC 1 cut(s) 21
Eco52I CGGCCG 1 cut(s) 399
Eco57I CTGAAG 1 cut(s) 962
Eco88I CYCGRG 2 cut(s) 113, 661
EcoRI GAATTC 1 cut(s) 404
EcoRII CCWGG 1 cut(s) 782
EcoT22I ATGCAT 1 cut(s) 840
EcoT38I GRGCYC 1 cut(s) 186
Esp3I CGTCTC 1 cut(s) 4
FaeI CATG 7 cut(s) 61, 110, 596, 659, 842, 1120, 1129
FaqI GGGAC 2 cut(s) 644, 801
FatI CATG 7 cut(s) 57, 106, 592, 655, 838, 1116, 1125
FauI CCCGC 1 cut(s) 257
FauNDI CATATG 1 cut(s) 1273
FbaI TGATCA 3 cut(s) 192, 652, 880
Fnu4HI GCNGC 2 cut(s) 399, 1184
FokI GGATG 5 cut(s) 130, 343, 572, 791, 1232
FriOI GRGCYC 1 cut(s) 186
Fsp4HI GCNGC 2 cut(s) 399, 1184
GluI GCNGC 2 cut(s) 399, 1184
GsuI CTGGAG 1 cut(s) 635
HaeIII GGCC 2 cut(s) 401, 644
HapII CCGG 3 cut(s) 24, 662, 798
HgaI GACGC 1 cut(s) 909
Hin1II CATG 7 cut(s) 61, 110, 596, 659, 842, 1120, 1129
HindIII AAGCTT 3 cut(s) 923, 1005, 1104
HinfI GANTC 4 cut(s) 103, 589, 789, 1223
HpaII CCGG 3 cut(s) 24, 662, 798
HphI GGTGA 4 cut(s) 38, 356, 875, 1220
Hpy166II GTNNAC 1 cut(s) 517
Hpy188I TCNGA 7 cut(s) 174, 262, 750, 970, 1021, 1159, 1243
Hpy8I GTNNAC 1 cut(s) 517
Hpy99I CGWCG 1 cut(s) 903
HpyAV CCTTC 3 cut(s) 994, 1048, 1195
HpyCH4III ACNGT 3 cut(s) 224, 288, 859
HpyCH4V TGCA 4 cut(s) 79, 838, 1183, 1266
HpyF10VI GCNNNNNNNGC 5 cut(s) 382, 844, 922, 931, 1189
HpyF3I CTNAG 4 cut(s) 126, 639, 848, 969
Hsp92II CATG 7 cut(s) 61, 110, 596, 659, 842, 1120, 1129
KroI GCCGGC 1 cut(s) 797
KroNI GCCGGC 1 cut(s) 799
Ksp22I TGATCA 3 cut(s) 192, 652, 880
KspI CCGCGG 1 cut(s) 399
Kzo9I GATC 9 cut(s) 109, 192, 214, 304, 315, 633, 652, 739, 880
LmnI GCTCC 3 cut(s) 382, 731, 1021
Lsp1109I GCAGC 1 cut(s) 1195
LweI GCATC 5 cut(s) 152, 394, 769, 903, 963
MaeIII GTNAC 4 cut(s) 121, 409, 874, 1112
MalI GATC 9 cut(s) 111, 194, 216, 306, 317, 635, 654, 741, 882
MboI GATC 9 cut(s) 109, 192, 214, 304, 315, 633, 652, 739, 880
MfeI CAATTG 1 cut(s) 209
MflI RGATCY 1 cut(s) 315
MhlI GDGCHC 1 cut(s) 186
MlyI GAGTC 1 cut(s) 783
MmeI TCCRAC 1 cut(s) 1194
Mph1103I ATGCAT 1 cut(s) 840
MroNI GCCGGC 1 cut(s) 797
MroXI GAANNNNTTC 2 cut(s) 53, 1139
MseI TTAA 3 cut(s) 546, 582, 1294
MspA1I CMGCKG 1 cut(s) 398
MspI CCGG 3 cut(s) 24, 662, 798
MspR9I CCNGG 3 cut(s) 662, 663, 784
MunI CAATTG 1 cut(s) 209
Mva1269I GAATGC 1 cut(s) 378
MvaI CCWGG 1 cut(s) 784
MvnI CGCG 3 cut(s) 398, 776, 903
MwoI GCNNNNNNNGC 5 cut(s) 382, 844, 922, 931, 1189
NaeI GCCGGC 1 cut(s) 799
NciI CCSGG 2 cut(s) 662, 663
NdeI CATATG 1 cut(s) 1273
NdeII GATC 9 cut(s) 109, 192, 214, 304, 315, 633, 652, 739, 880
NgoMIV GCCGGC 1 cut(s) 797
NlaIII CATG 7 cut(s) 61, 110, 596, 659, 842, 1120, 1129
NlaIV GGNNCC 1 cut(s) 733
NmuCI GTSAC 3 cut(s) 121, 409, 1112
NsiI ATGCAT 1 cut(s) 840
NspV TTCGAA 1 cut(s) 271
PaeR7I CTCGAG 1 cut(s) 113
PagI TCATGA 2 cut(s) 57, 106
PctI GAATGC 1 cut(s) 378
PdiI GCCGGC 1 cut(s) 799
PdmI GAANNNNTTC 2 cut(s) 53, 1139
PfeI GAWTC 3 cut(s) 103, 589, 1223
PkrI GCNGC 2 cut(s) 400, 1185
PleI GAGTC 1 cut(s) 783
PpsI GAGTC 1 cut(s) 783
PsiI TTATAA 1 cut(s) 708
Psp6I CCWGG 1 cut(s) 782
PspGI CCWGG 1 cut(s) 782
PspN4I GGNNCC 1 cut(s) 733
PspPI GGNCC 1 cut(s) 21
PsuI RGATCY 1 cut(s) 315
RsaI GTAC 5 cut(s) 221, 299, 357, 719, 725
RsaNI GTAC 5 cut(s) 220, 298, 356, 718, 724
SacII CCGCGG 1 cut(s) 399
SaqAI TTAA 3 cut(s) 546, 582, 1294
SatI GCNGC 2 cut(s) 399, 1184
Sau3AI GATC 9 cut(s) 109, 192, 214, 304, 315, 633, 652, 739, 880
Sau96I GGNCC 1 cut(s) 21
SchI GAGTC 1 cut(s) 783
ScrFI CCNGG 3 cut(s) 662, 663, 784
SduI GDGCHC 1 cut(s) 186
SfaNI GCATC 5 cut(s) 152, 394, 769, 903, 963
Sfr274I CTCGAG 1 cut(s) 113
Sfr303I CCGCGG 1 cut(s) 399
SfuI TTCGAA 1 cut(s) 271
SgrBI CCGCGG 1 cut(s) 399
SinI GGWCC 1 cut(s) 21
SlaI CTCGAG 1 cut(s) 113
SmaI CCCGGG 1 cut(s) 663
SmlI CTYRAG 3 cut(s) 113, 377, 1187
SmoI CTYRAG 3 cut(s) 113, 377, 1187
SsiI CCGC 6 cut(s) 52, 250, 396, 398, 776, 1232
SspI AATATT 1 cut(s) 533
StyD4I CCNGG 3 cut(s) 660, 661, 782
TaaI ACNGT 3 cut(s) 224, 288, 859
TaqI TCGA 5 cut(s) 114, 271, 435, 738, 898
TatI WGTACW 2 cut(s) 297, 723
TauI GCSGC 1 cut(s) 401
TfiI GAWTC 3 cut(s) 103, 589, 1223
Tru1I TTAA 3 cut(s) 546, 582, 1294
Tru9I TTAA 3 cut(s) 546, 582, 1294
TscAI CASTG 3 cut(s) 643, 652, 1117
TseFI GTSAC 3 cut(s) 121, 409, 1112
TseI GCWGC 1 cut(s) 1183
Tsp45I GTSAC 3 cut(s) 121, 409, 1112
TspDTI ATGAA 3 cut(s) 46, 56, 95
TspGWI ACGGA 1 cut(s) 675
TspMI CCCGGG 1 cut(s) 661
TspRI CASTG 3 cut(s) 643, 652, 1117
VpaK11BI GGWCC 1 cut(s) 21
XapI RAATTY 3 cut(s) 168, 404, 809
XhoI CTCGAG 1 cut(s) 113
XmaI CCCGGG 1 cut(s) 661
XmnI GAANNNNTTC 2 cut(s) 53, 1139
Zsp2I ATGCAT 1 cut(s) 840
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.