pycom14g12660

Protein ROOT PRIMORDIUM DEFECTIVE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0009141
Physical Location & Seq
Reverse (-)
15700310 .. 15701605
1296 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g12660.1

Sequence Viewer

Length: 1296 bp
ATGTTTGTGAGACGATACTGGTCCGGTGAGTGGTGGCCAATGAACCAGAAGCGGTTCATGACCACCAGCAAGAGGGTGCAAGACAGAAGCAAATTGAAGAGGATTCATGATCTCGAGATTGTGACTGAGAAATGGAAGATAGCATCCAAAGTGTTGTTCTTGATGGAAATTCTGAAAAAAGAGCCCGAAATGATCATTCCCGTCAGGCAATTGGATCAGTACCGCAGCCAGATTAGTCTCCCAAAGCCCAACAGGGTGTCTGATTTTATTCGAAAATCGCCGAAACTGTTTGAATTGTACAAGGATCACAGAGGGATCTTATGGTGTGGGATGACCAAGCAAGGTGAGGAATTGGTACAAGAGGAGGACAGAATACTTGAGGAGCATCAAGATACGGCGGCCGAATTCGTGACACGCTTTCTTATGATGTCTGTAGATAAAAGGCTGGCATTGGACAAGATTGTCCATTTCAGGAGGGATTTTGCTCTGCCAGTTGATTTCAGGACTAATTGGGTGCACAAATATCCCCAACATTTCAAGGTGGTTAAAGATAATGAGGAAGTTGAGCATTTGGAACTTGTTAATTGGAATCCTGCATGGGCTATAACCGAGTTGGAGAAGAAGACAATGGGGATCACTGAGGCCAGTGATGATCATGACCCGGGCTTGCTTTTGCTCTCTTTTCCGTTGAAGTTCCCCCCGAATTACAAAAAGGCGTACAAGTACAAAGGAGCCATTGATCATTTTCAGAAAAGGTCTTATTTATCTCCATATGCAGATGCCATGGGACTCAAAGCCGGCTCGAAAGAATTTGACAAGAGGGCAGTTGCTATTATGCATGAGTTGCTTAGCTTTACTGTGGAGAAGAGATTGGTTACTGATCACCTCACTCATTTTCGACGCGAACTTGTGATGCCCCAGAAGCTTATGAGGCTTCTACTGAAGCATTTTGGCATCTTCTATGTTTCAGAGAGGGGTAAGAGGTTTAGCGTGTTCTTGAAAGAAGCTTACAAGGGTTCGGACCTGATTGAGAAATGCCCTTTGGTGGTTTGGAAGGAAAAAGTCCAGAGCCTTATTGGTTATAGGGGAAAGAAGAAGATTGAAGCTTTTAGTGACATGGAAGACATGGAAGAGAATGGTTTCCATGAGAGCAATTCAGAAAGTGAGGACACCATTGACTTGCAGCTTGAGCAAGAGGAGAAAGTAGGTGACTTGGAGGATGATTCACTTGCAGGTTATTCTGAAATGGAGATTAAAGAGATTTGCAGTGCATATGAGGATGCTAAAAGATGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PORR

Protein Analysis

432

Amino Acids

51.06

Weight (kDa)

7.24

Isoelectric Point (pI)

43.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PORR PF11955 33 - 361 4.4e-107 Plant organelle RNA recognition domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 461
Acc36I ACCTGC 1 cut(s) 1223
AccII CGCG 1 cut(s) 903
AciI CCGC 3 cut(s) 52, 223, 398
AclWI GGATC 4 cut(s) 222, 312, 323, 641
AcoI YGGCCR 2 cut(s) 35, 399
AcsI RAATTY 3 cut(s) 168, 404, 809
AcuI CTGAAG 1 cut(s) 962
AfaI GTAC 5 cut(s) 221, 299, 357, 719, 725
AfiI CCNNNNNNNGG 3 cut(s) 30, 72, 1045
AgsI TTSAA 6 cut(s) 97, 293, 538, 691, 1000, 1103
AjuI GAANNNNNNNTTGG 4 cut(s) 140, 172, 1025, 1057
AluBI AGCT 5 cut(s) 852, 925, 1007, 1106, 1186
AluI AGCT 5 cut(s) 852, 925, 1007, 1106, 1186
Alw21I GWGCWC 1 cut(s) 519
Alw26I GTCTC 2 cut(s) 4, 242
Alw44I GTGCAC 1 cut(s) 515
AlwI GGATC 4 cut(s) 222, 312, 323, 641
Ama87I CYCGRG 2 cut(s) 113, 661
AoxI GGCC 3 cut(s) 35, 399, 642
ApaLI GTGCAC 1 cut(s) 515
ApeKI GCWGC 2 cut(s) 225, 1183
ApoI RAATTY 3 cut(s) 168, 404, 809
Asp700I GAANNNNTTC 2 cut(s) 53, 1139
AspS9I GGNCC 2 cut(s) 21, 1021
AsuC2I CCSGG 2 cut(s) 662, 663
AsuHPI GGTGA 4 cut(s) 38, 356, 875, 1220
AsuII TTCGAA 1 cut(s) 271
AvaI CYCGRG 2 cut(s) 113, 661
AvaII GGWCC 2 cut(s) 21, 1021
BaeGI GKGCMC 1 cut(s) 519
BalI TGGCCA 1 cut(s) 37
BanII GRGCYC 1 cut(s) 186
BbsI GAAGAC 2 cut(s) 629, 1128
Bbv12I GWGCWC 1 cut(s) 519
BbvI GCAGC 2 cut(s) 237, 1195
BccI CCATC 1 cut(s) 157
BceAI ACGGC 1 cut(s) 411
BclI TGATCA 4 cut(s) 192, 652, 739, 880
BcnI CCSGG 2 cut(s) 662, 663
BcoDI GTCTC 2 cut(s) 4, 242
BfmI CTRYAG 1 cut(s) 432
BfuAI ACCTGC 1 cut(s) 1223
BisI GCNGC 3 cut(s) 226, 399, 1184
BlpI GCTNAGC 1 cut(s) 848
BlsI GCNGC 3 cut(s) 227, 400, 1185
Bme1390I CCNGG 2 cut(s) 662, 663
Bme18I GGWCC 2 cut(s) 21, 1021
BmeT110I CYCGRG 2 cut(s) 113, 661
BmgT120I GGNCC 2 cut(s) 21, 1021
BmiI GGNNCC 1 cut(s) 733
BmrFI CCNGG 2 cut(s) 662, 663
BmsI GCATC 6 cut(s) 152, 394, 769, 903, 963, 1270
BpiI GAAGAC 2 cut(s) 629, 1128
Bpu1102I GCTNAGC 1 cut(s) 848
Bpu14I TTCGAA 1 cut(s) 271
BpuEI CTTGAG 2 cut(s) 398, 1208
BpuMI CCSGG 2 cut(s) 662, 663
BsaJI CCNNGG 2 cut(s) 661, 783
BsaWI WCCGGW 1 cut(s) 23
Bsc4I CCNNNNNNNGG 3 cut(s) 30, 72, 1045
Bse118I RCCGGY 1 cut(s) 797
Bse1I ACTGG 3 cut(s) 23, 491, 645
BseDI CCNNGG 2 cut(s) 661, 783
BseGI GGATG 4 cut(s) 143, 336, 1225, 1285
BseLI CCNNNNNNNGG 3 cut(s) 30, 72, 1045
BseMII CTCAG 2 cut(s) 117, 630
BseNI ACTGG 3 cut(s) 23, 491, 645
BseRI GAGGAG 3 cut(s) 377, 395, 1211
BseSI GKGCMC 1 cut(s) 519
BseX3I CGGCCG 1 cut(s) 399
BseXI GCAGC 2 cut(s) 237, 1195
Bsh1236I CGCG 1 cut(s) 903
Bsh1285I CGRYCG 1 cut(s) 402
BshFI GGCC 3 cut(s) 37, 401, 644
BsiEI CGRYCG 1 cut(s) 402
BsiHKAI GWGCWC 1 cut(s) 519
BsiHKCI CYCGRG 2 cut(s) 113, 661
BsiSI CCGG 3 cut(s) 24, 662, 798
BslFI GGGAC 1 cut(s) 801
BslI CCNNNNNNNGG 3 cut(s) 30, 72, 1045
BsmAI GTCTC 2 cut(s) 4, 242
BsmBI CGTCTC 1 cut(s) 4
BsmFI GGGAC 1 cut(s) 801
BsnI GGCC 3 cut(s) 37, 401, 644
BsoBI CYCGRG 2 cut(s) 113, 661
Bsp119I TTCGAA 1 cut(s) 271
Bsp1286I GDGCHC 2 cut(s) 186, 519
Bsp1407I TGTACA 1 cut(s) 297
Bsp143I GATC 9 cut(s) 109, 192, 214, 304, 315, 633, 652, 739, 880
Bsp1720I GCTNAGC 1 cut(s) 848
Bsp19I CCATGG 1 cut(s) 783
BspACI CCGC 3 cut(s) 52, 223, 398
BspANI GGCC 3 cut(s) 37, 401, 644
BspCNI CTCAG 2 cut(s) 118, 631
BspFNI CGCG 1 cut(s) 903
BspHI TCATGA 3 cut(s) 57, 106, 655
BspLI GGNNCC 1 cut(s) 733
BspMI ACCTGC 1 cut(s) 1223
BspPI GGATC 4 cut(s) 222, 312, 323, 641
BspT104I TTCGAA 1 cut(s) 271
BsrFI RCCGGY 1 cut(s) 797
BsrGI TGTACA 1 cut(s) 297
BsrI ACTGG 3 cut(s) 23, 491, 645
BssAI RCCGGY 1 cut(s) 797
BssECI CCNNGG 2 cut(s) 661, 783
BssMI GATC 9 cut(s) 109, 192, 214, 304, 315, 633, 652, 739, 880
BssT1I CCWWGG 1 cut(s) 783
Bst4CI ACNGT 2 cut(s) 288, 859
Bst6I CTCTTC 3 cut(s) 92, 860, 1125
BstAPI GCANNNNNTGC 1 cut(s) 844
BstAUI TGTACA 1 cut(s) 297
BstBI TTCGAA 1 cut(s) 271
BstC8I GCNNGC 3 cut(s) 447, 668, 799
BstDEI CTNAG 3 cut(s) 126, 639, 848
BstDSI CCRYGG 1 cut(s) 783
BstF5I GGATG 4 cut(s) 143, 336, 1225, 1285
BstFNI CGCG 1 cut(s) 903
BstKTI GATC 9 cut(s) 112, 195, 217, 307, 318, 636, 655, 742, 883
BstMAI GTCTC 2 cut(s) 4, 242
BstMBI GATC 9 cut(s) 109, 192, 214, 304, 315, 633, 652, 739, 880
BstMCI CGRYCG 1 cut(s) 402
BstMWI GCNNNNNNNGC 4 cut(s) 844, 922, 931, 1189
BstSCI CCNGG 2 cut(s) 660, 661
BstSFI CTRYAG 1 cut(s) 432
BstSLI GKGCMC 1 cut(s) 519
BstUI CGCG 1 cut(s) 903
BstV1I GCAGC 2 cut(s) 237, 1195
BstV2I GAAGAC 2 cut(s) 629, 1128
BstX2I RGATCY 1 cut(s) 315
BstYI RGATCY 1 cut(s) 315
BstZI CGGCCG 1 cut(s) 399
BsuRI GGCC 3 cut(s) 37, 401, 644
BtgI CCRYGG 1 cut(s) 783
BtsCI GGATG 4 cut(s) 143, 336, 1225, 1285
BtsI GCAGTG 1 cut(s) 1273
BtsIMutI CAGTG 3 cut(s) 636, 652, 1273
BveI ACCTGC 1 cut(s) 1223
Cac8I GCNNGC 3 cut(s) 447, 668, 799
CciI TCATGA 3 cut(s) 57, 106, 655
Cfr10I RCCGGY 1 cut(s) 797
Cfr13I GGNCC 2 cut(s) 21, 1021
Cfr9I CCCGGG 1 cut(s) 661
CseI GACGC 1 cut(s) 909
Csp6I GTAC 5 cut(s) 220, 298, 356, 718, 724
CviAII CATG 9 cut(s) 58, 107, 597, 656, 784, 839, 1117, 1126, 1145
CviQI GTAC 5 cut(s) 220, 298, 356, 718, 724
DdeI CTNAG 3 cut(s) 126, 639, 848
DpnI GATC 9 cut(s) 111, 194, 216, 306, 317, 635, 654, 741, 882
DpnII GATC 9 cut(s) 109, 192, 214, 304, 315, 633, 652, 739, 880
DrdI GACNNNNNNGTC 1 cut(s) 461
DseDI GACNNNNNNGTC 1 cut(s) 461
EaeI YGGCCR 2 cut(s) 35, 399
EagI CGGCCG 1 cut(s) 399
Eam1104I CTCTTC 3 cut(s) 92, 860, 1125
EarI CTCTTC 3 cut(s) 92, 860, 1125
EclXI CGGCCG 1 cut(s) 399
Eco130I CCWWGG 1 cut(s) 783
Eco24I GRGCYC 1 cut(s) 186
Eco47I GGWCC 2 cut(s) 21, 1021
Eco52I CGGCCG 1 cut(s) 399
Eco57I CTGAAG 1 cut(s) 962
Eco88I CYCGRG 2 cut(s) 113, 661
EcoRI GAATTC 1 cut(s) 404
EcoT14I CCWWGG 1 cut(s) 783
EcoT22I ATGCAT 1 cut(s) 840
EcoT38I GRGCYC 1 cut(s) 186
ErhI CCWWGG 1 cut(s) 783
Esp3I CGTCTC 1 cut(s) 4
FaeI CATG 9 cut(s) 61, 110, 600, 659, 787, 842, 1120, 1129, 1148
FaqI GGGAC 1 cut(s) 801
FatI CATG 9 cut(s) 57, 106, 596, 655, 783, 838, 1116, 1125, 1144
FauNDI CATATG 2 cut(s) 772, 1273
FbaI TGATCA 4 cut(s) 192, 652, 739, 880
Fnu4HI GCNGC 3 cut(s) 226, 399, 1184
FokI GGATG 4 cut(s) 130, 343, 1232, 1292
FriOI GRGCYC 1 cut(s) 186
Fsp4HI GCNGC 3 cut(s) 226, 399, 1184
GluI GCNGC 3 cut(s) 226, 399, 1184
HaeIII GGCC 3 cut(s) 37, 401, 644
HapII CCGG 3 cut(s) 24, 662, 798
HgaI GACGC 1 cut(s) 909
Hin1II CATG 9 cut(s) 61, 110, 600, 659, 787, 842, 1120, 1129, 1148
HindIII AAGCTT 3 cut(s) 923, 1005, 1104
HinfI GANTC 4 cut(s) 103, 589, 789, 1223
HpaII CCGG 3 cut(s) 24, 662, 798
HphI GGTGA 4 cut(s) 38, 356, 875, 1220
Hpy166II GTNNAC 1 cut(s) 517
Hpy188I TCNGA 7 cut(s) 174, 262, 750, 970, 1021, 1159, 1243
Hpy8I GTNNAC 1 cut(s) 517
Hpy99I CGWCG 1 cut(s) 903
HpyAV CCTTC 1 cut(s) 1048
HpyCH4III ACNGT 2 cut(s) 288, 859
HpyCH4V TGCA 9 cut(s) 79, 517, 596, 776, 838, 1183, 1232, 1266, 1271
HpyF10VI GCNNNNNNNGC 4 cut(s) 844, 922, 931, 1189
HpyF3I CTNAG 3 cut(s) 126, 639, 848
Hsp92II CATG 9 cut(s) 61, 110, 600, 659, 787, 842, 1120, 1129, 1148
KroI GCCGGC 1 cut(s) 797
KroNI GCCGGC 1 cut(s) 799
Ksp22I TGATCA 4 cut(s) 192, 652, 739, 880
Kzo9I GATC 9 cut(s) 109, 192, 214, 304, 315, 633, 652, 739, 880
LmnI GCTCC 2 cut(s) 382, 731
Lsp1109I GCAGC 2 cut(s) 237, 1195
LweI GCATC 6 cut(s) 152, 394, 769, 903, 963, 1270
MaeIII GTNAC 5 cut(s) 121, 409, 874, 1112, 1208
MalI GATC 9 cut(s) 111, 194, 216, 306, 317, 635, 654, 741, 882
MboI GATC 9 cut(s) 109, 192, 214, 304, 315, 633, 652, 739, 880
MfeI CAATTG 1 cut(s) 209
MflI RGATCY 1 cut(s) 315
MhlI GDGCHC 2 cut(s) 186, 519
MlsI TGGCCA 1 cut(s) 37
MluNI TGGCCA 1 cut(s) 37
MlyI GAGTC 1 cut(s) 783
MmeI TCCRAC 1 cut(s) 594
Mox20I TGGCCA 1 cut(s) 37
Mph1103I ATGCAT 1 cut(s) 840
MroNI GCCGGC 1 cut(s) 797
MroXI GAANNNNTTC 2 cut(s) 53, 1139
MscI TGGCCA 1 cut(s) 37
MseI TTAA 4 cut(s) 546, 582, 1254, 1294
Msp20I TGGCCA 1 cut(s) 37
MspI CCGG 3 cut(s) 24, 662, 798
MspR9I CCNGG 2 cut(s) 662, 663
MunI CAATTG 1 cut(s) 209
MvnI CGCG 1 cut(s) 903
MwoI GCNNNNNNNGC 4 cut(s) 844, 922, 931, 1189
NaeI GCCGGC 1 cut(s) 799
NciI CCSGG 2 cut(s) 662, 663
NcoI CCATGG 1 cut(s) 783
NdeI CATATG 2 cut(s) 772, 1273
NdeII GATC 9 cut(s) 109, 192, 214, 304, 315, 633, 652, 739, 880
NgoMIV GCCGGC 1 cut(s) 797
NlaIII CATG 9 cut(s) 61, 110, 600, 659, 787, 842, 1120, 1129, 1148
NlaIV GGNNCC 1 cut(s) 733
NmuCI GTSAC 4 cut(s) 121, 409, 1112, 1208
NsiI ATGCAT 1 cut(s) 840
NspV TTCGAA 1 cut(s) 271
PaeR7I CTCGAG 1 cut(s) 113
PagI TCATGA 3 cut(s) 57, 106, 655
PdiI GCCGGC 1 cut(s) 799
PdmI GAANNNNTTC 2 cut(s) 53, 1139
PfeI GAWTC 3 cut(s) 103, 589, 1223
PkrI GCNGC 3 cut(s) 227, 400, 1185
PleI GAGTC 1 cut(s) 783
PpsI GAGTC 1 cut(s) 783
PspN4I GGNNCC 1 cut(s) 733
PspPI GGNCC 2 cut(s) 21, 1021
PsuI RGATCY 1 cut(s) 315
RsaI GTAC 5 cut(s) 221, 299, 357, 719, 725
RsaNI GTAC 5 cut(s) 220, 298, 356, 718, 724
SaqAI TTAA 4 cut(s) 546, 582, 1254, 1294
SatI GCNGC 3 cut(s) 226, 399, 1184
Sau3AI GATC 9 cut(s) 109, 192, 214, 304, 315, 633, 652, 739, 880
Sau96I GGNCC 2 cut(s) 21, 1021
SchI GAGTC 1 cut(s) 783
ScrFI CCNGG 2 cut(s) 662, 663
SduI GDGCHC 2 cut(s) 186, 519
SfaNI GCATC 6 cut(s) 152, 394, 769, 903, 963, 1270
SfcI CTRYAG 1 cut(s) 432
Sfr274I CTCGAG 1 cut(s) 113
SfuI TTCGAA 1 cut(s) 271
SinI GGWCC 2 cut(s) 21, 1021
SlaI CTCGAG 1 cut(s) 113
SmaI CCCGGG 1 cut(s) 663
SmlI CTYRAG 3 cut(s) 113, 377, 1187
SmoI CTYRAG 3 cut(s) 113, 377, 1187
SsiI CCGC 3 cut(s) 52, 223, 398
StyD4I CCNGG 2 cut(s) 660, 661
StyI CCWWGG 1 cut(s) 783
TaaI ACNGT 2 cut(s) 288, 859
TaqI TCGA 4 cut(s) 114, 271, 803, 898
TatI WGTACW 2 cut(s) 297, 723
TauI GCSGC 1 cut(s) 401
TfiI GAWTC 3 cut(s) 103, 589, 1223
Tru1I TTAA 4 cut(s) 546, 582, 1254, 1294
Tru9I TTAA 4 cut(s) 546, 582, 1254, 1294
TscAI CASTG 3 cut(s) 643, 652, 1273
TseFI GTSAC 4 cut(s) 121, 409, 1112, 1208
TseI GCWGC 2 cut(s) 225, 1183
Tsp45I GTSAC 4 cut(s) 121, 409, 1112, 1208
TspDTI ATGAA 3 cut(s) 46, 56, 95
TspGWI ACGGA 1 cut(s) 675
TspMI CCCGGG 1 cut(s) 661
TspRI CASTG 3 cut(s) 643, 652, 1273
VneI GTGCAC 1 cut(s) 515
VpaK11BI GGWCC 2 cut(s) 21, 1021
XapI RAATTY 3 cut(s) 168, 404, 809
XcmI CCANNNNNNNNNTGG 1 cut(s) 1073
XhoI CTCGAG 1 cut(s) 113
XmaI CCCGGG 1 cut(s) 661
XmnI GAANNNNTTC 2 cut(s) 53, 1139
Zsp2I ATGCAT 1 cut(s) 840
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.