Rh2AG301200

Protein ROOT PRIMORDIUM DEFECTIVE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0009141
Physical Location & Seq
Forward (+)
37624338 .. 37627377
3040 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG301200.1

Sequence Viewer

Length: 741 bp
ATGTTTGCGAGAAGATACTGGTCCTCCATTGGTTGGTGGTCGAAGAATCAGAATCGGTTCATGACCACCAGTAAGCGTGTGCAGGACAGGAGCAAATTGAAGAGGGTTCATGATCTTGAAATTGTCACTGAGAAATGGAAGATAGCTTCCAAGGTGCTTTTTTTGATGGAATTACTCAAGAAGGAGCCGGAAATGATAATTCCGGTGAGGTCATTGGACAGGTTCCGGGTGCAGATTAGTCTTCCTAAGCCCCATAGGATGTCTGATTTCATTAGGAAATCGCTGAAACTGTTTGAATTGTATAGGGTTCGTAGGGGAGTTTTACGGTGTGGGATGACTGAGCAAGCTGAGGAGTTGGTAGAGGAGGAGGATAGGTTACTTGAGCAGCATCAAGATAAGGCTGCTGAGTATGTTACTCGTTTTCTTATGATGTCTGTTGATAAGCGGCTTCGAGTTGACAAGATTGCTCATTATAGGAAAGACTTTGCCCTTCCAAATGATTTTAGGACTACCTGGGTGCACAAGCATCCCCAACACTTTAGGCTGGTAAGGGATAATGAGGATATCGAGTACTTGGAACTTGTTGCTTGGAATCCTGCTTGGGCTATAACGGAGTTGGAGAAGAAGACAATGGGGGTTACTGATGCCGGTGCTCATACCCCTGGCTTGCTTTCACTCTCGTTTCCTTTGAAGTTCCCTCCAAATTACAAGAAGAGTGTACAAATATCGGGGAGCGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PORR

Protein Analysis

246

Amino Acids

29.29

Weight (kDa)

9.81

Isoelectric Point (pI)

37.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PORR PF11955 34 - 241 1.9e-51 Plant organelle RNA recognition domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 33
AciI CCGC 1 cut(s) 445
AfaI GTAC 2 cut(s) 572, 720
AfeI AGCGCT 1 cut(s) 736
AfiI CCNNNNNNNGG 1 cut(s) 33
AgsI TTSAA 4 cut(s) 100, 119, 296, 691
AjnI CCWGG 2 cut(s) 512, 661
AluBI AGCT 2 cut(s) 146, 347
AluI AGCT 2 cut(s) 146, 347
Alw21I GWGCWC 2 cut(s) 522, 655
Alw44I GTGCAC 1 cut(s) 518
Aor51HI AGCGCT 1 cut(s) 736
ApaLI GTGCAC 1 cut(s) 518
ApeKI GCWGC 2 cut(s) 385, 401
Asp700I GAANNNNTTC 1 cut(s) 56
AspLEI GCGC 1 cut(s) 737
AspS9I GGNCC 1 cut(s) 21
AsuC2I CCSGG 1 cut(s) 227
AsuHPI GGTGA 1 cut(s) 217
AvaII GGWCC 1 cut(s) 21
BaeGI GKGCMC 1 cut(s) 522
BbsI GAAGAC 2 cut(s) 233, 632
Bbv12I GWGCWC 2 cut(s) 522, 655
BbvCI CCTCAGC 1 cut(s) 348
BbvI GCAGC 2 cut(s) 388, 397
BccI CCATC 1 cut(s) 160
BciT130I CCWGG 2 cut(s) 514, 663
BcnI CCSGG 1 cut(s) 227
BfoI RGCGCY 1 cut(s) 738
BisI GCNGC 3 cut(s) 386, 402, 446
BlsI GCNGC 3 cut(s) 387, 403, 447
BmcAI AGTACT 1 cut(s) 572
Bme1390I CCNGG 3 cut(s) 227, 514, 663
Bme18I GGWCC 1 cut(s) 21
BmgT120I GGNCC 1 cut(s) 21
BmiI GGNNCC 2 cut(s) 186, 224
BmrFI CCNGG 3 cut(s) 227, 514, 663
BmsI GCATC 3 cut(s) 397, 535, 634
BpiI GAAGAC 2 cut(s) 233, 632
Bpu10I CCTNAGC 2 cut(s) 246, 348
BpuEI CTTGAG 2 cut(s) 161, 401
BpuMI CCSGG 1 cut(s) 227
BsaJI CCNNGG 3 cut(s) 150, 513, 661
BsaWI WCCGGW 1 cut(s) 202
BsaXI ACNNNNNCTCC 4 cut(s) 8, 38, 359, 389
Bsc4I CCNNNNNNNGG 1 cut(s) 33
Bse118I RCCGGY 1 cut(s) 647
Bse1I ACTGG 2 cut(s) 23, 69
BseBI CCWGG 2 cut(s) 514, 663
BseDI CCNNGG 3 cut(s) 150, 513, 661
BseGI GGATG 3 cut(s) 264, 339, 526
BseLI CCNNNNNNNGG 1 cut(s) 33
BseMII CTCAG 4 cut(s) 120, 330, 339, 396
BseNI ACTGG 2 cut(s) 23, 69
BseRI GAGGAG 3 cut(s) 365, 377, 380
BseSI GKGCMC 1 cut(s) 522
BseXI GCAGC 2 cut(s) 388, 397
BsgI GTGCAG 2 cut(s) 101, 251
BsiHKAI GWGCWC 2 cut(s) 522, 655
BsiSI CCGG 4 cut(s) 188, 203, 226, 648
BslI CCNNNNNNNGG 1 cut(s) 33
Bsp1286I GDGCHC 2 cut(s) 522, 655
Bsp1407I TGTACA 1 cut(s) 718
Bsp143I GATC 1 cut(s) 112
BspACI CCGC 1 cut(s) 445
BspCNI CTCAG 4 cut(s) 121, 331, 340, 397
BspHI TCATGA 2 cut(s) 60, 109
BspLI GGNNCC 2 cut(s) 186, 224
BsrFI RCCGGY 1 cut(s) 647
BsrGI TGTACA 1 cut(s) 718
BsrI ACTGG 2 cut(s) 23, 69
BssAI RCCGGY 1 cut(s) 647
BssECI CCNNGG 3 cut(s) 150, 513, 661
BssMI GATC 1 cut(s) 112
BssT1I CCWWGG 1 cut(s) 150
Bst2UI CCWGG 2 cut(s) 514, 663
Bst4CI ACNGT 2 cut(s) 291, 327
Bst6I CTCTTC 2 cut(s) 95, 707
BstAUI TGTACA 1 cut(s) 718
BstC8I GCNNGC 2 cut(s) 345, 668
BstDEI CTNAG 5 cut(s) 129, 246, 339, 348, 405
BstF5I GGATG 3 cut(s) 264, 339, 526
BstH2I RGCGCY 1 cut(s) 738
BstHHI GCGC 1 cut(s) 737
BstKTI GATC 1 cut(s) 115
BstMBI GATC 1 cut(s) 112
BstNI CCWGG 2 cut(s) 514, 663
BstSCI CCNGG 3 cut(s) 225, 512, 661
BstSLI GKGCMC 1 cut(s) 522
BstV1I GCAGC 2 cut(s) 388, 397
BstV2I GAAGAC 2 cut(s) 233, 632
BtsCI GGATG 3 cut(s) 264, 339, 526
BtsIMutI CAGTG 1 cut(s) 126
Cac8I GCNNGC 2 cut(s) 345, 668
CciI TCATGA 2 cut(s) 60, 109
CfoI GCGC 1 cut(s) 737
Cfr10I RCCGGY 1 cut(s) 647
Cfr13I GGNCC 1 cut(s) 21
Csp6I GTAC 2 cut(s) 571, 719
CviAII CATG 2 cut(s) 61, 110
CviJI RGCY 9 cut(s) 146, 187, 250, 347, 401, 448, 544, 605, 666
CviKI_1 RGCY 9 cut(s) 146, 187, 250, 347, 401, 448, 544, 605, 666
CviQI GTAC 2 cut(s) 571, 719
DdeI CTNAG 5 cut(s) 129, 246, 339, 348, 405
DpnI GATC 1 cut(s) 114
DpnII GATC 1 cut(s) 112
Eam1104I CTCTTC 2 cut(s) 95, 707
EarI CTCTTC 2 cut(s) 95, 707
Eco130I CCWWGG 1 cut(s) 150
Eco32I GATATC 1 cut(s) 565
Eco47I GGWCC 1 cut(s) 21
Eco47III AGCGCT 1 cut(s) 736
EcoRII CCWGG 2 cut(s) 512, 661
EcoRV GATATC 1 cut(s) 565
EcoT14I CCWWGG 1 cut(s) 150
ErhI CCWWGG 1 cut(s) 150
FaeI CATG 2 cut(s) 64, 113
FaiI YATR 9 cut(s) 62, 111, 255, 303, 411, 428, 474, 608, 657
FatI CATG 2 cut(s) 60, 109
Fnu4HI GCNGC 3 cut(s) 386, 402, 446
FokI GGATG 3 cut(s) 271, 346, 513
Fsp4HI GCNGC 3 cut(s) 386, 402, 446
GlaI GCGC 1 cut(s) 736
GluI GCNGC 3 cut(s) 386, 402, 446
HaeII RGCGCY 1 cut(s) 738
HapII CCGG 4 cut(s) 188, 203, 226, 648
HhaI GCGC 1 cut(s) 737
Hin1II CATG 2 cut(s) 64, 113
Hin6I GCGC 1 cut(s) 735
HinP1I GCGC 1 cut(s) 735
HincII GTYRAC 1 cut(s) 457
HindII GTYRAC 1 cut(s) 457
HinfI GANTC 3 cut(s) 46, 52, 592
HpaII CCGG 4 cut(s) 188, 203, 226, 648
HphI GGTGA 1 cut(s) 217
Hpy166II GTNNAC 3 cut(s) 457, 520, 719
Hpy188I TCNGA 2 cut(s) 51, 265
Hpy188III TCNNGA 5 cut(s) 61, 110, 116, 178, 392
Hpy8I GTNNAC 3 cut(s) 457, 520, 719
HpyAV CCTTC 2 cut(s) 175, 500
HpyCH4III ACNGT 2 cut(s) 291, 327
HpyCH4V TGCA 3 cut(s) 82, 232, 520
HpyF3I CTNAG 5 cut(s) 129, 246, 339, 348, 405
Hsp92II CATG 2 cut(s) 64, 113
HspAI GCGC 1 cut(s) 735
Kzo9I GATC 1 cut(s) 112
LmnI GCTCC 3 cut(s) 90, 184, 732
Lsp1109I GCAGC 2 cut(s) 388, 397
LweI GCATC 3 cut(s) 397, 535, 634
MaeIII GTNAC 4 cut(s) 124, 375, 412, 637
MalI GATC 1 cut(s) 114
MboI GATC 1 cut(s) 112
MboII GAAGA 8 cut(s) 24, 55, 112, 151, 233, 634, 637, 724
MhlI GDGCHC 2 cut(s) 522, 655
MluCI AATT 6 cut(s) 95, 120, 170, 198, 296, 703
MmeI TCCRAC 1 cut(s) 597
MnlI CCTC 9 cut(s) 34, 96, 201, 343, 355, 358, 361, 553, 708
MroXI GAANNNNTTC 1 cut(s) 56
MspI CCGG 4 cut(s) 188, 203, 226, 648
MspR9I CCNGG 3 cut(s) 227, 514, 663
MvaI CCWGG 2 cut(s) 514, 663
NciI CCSGG 1 cut(s) 227
NdeII GATC 1 cut(s) 112
NlaIII CATG 2 cut(s) 64, 113
NlaIV GGNNCC 2 cut(s) 186, 224
NmuCI GTSAC 1 cut(s) 124
PagI TCATGA 2 cut(s) 60, 109
PdmI GAANNNNTTC 1 cut(s) 56
PfeI GAWTC 3 cut(s) 46, 52, 592
PflMI CCANNNNNTGG 1 cut(s) 33
PkrI GCNGC 3 cut(s) 387, 403, 447
Psp6I CCWGG 2 cut(s) 512, 661
PspGI CCWGG 2 cut(s) 512, 661
PspN4I GGNNCC 2 cut(s) 186, 224
PspPI GGNCC 1 cut(s) 21
RsaI GTAC 2 cut(s) 572, 720
RsaNI GTAC 2 cut(s) 571, 719
SatI GCNGC 3 cut(s) 386, 402, 446
Sau3AI GATC 1 cut(s) 112
Sau96I GGNCC 1 cut(s) 21
ScaI AGTACT 1 cut(s) 572
ScrFI CCNGG 3 cut(s) 227, 514, 663
SduI GDGCHC 2 cut(s) 522, 655
SetI ASST 7 cut(s) 148, 156, 212, 224, 349, 377, 515
SfaNI GCATC 3 cut(s) 397, 535, 634
SinI GGWCC 1 cut(s) 21
SmlI CTYRAG 2 cut(s) 176, 380
SmoI CTYRAG 2 cut(s) 176, 380
Sse9I AATT 6 cut(s) 95, 120, 170, 198, 296, 703
SsiI CCGC 1 cut(s) 445
StyD4I CCNGG 3 cut(s) 225, 512, 661
StyI CCWWGG 1 cut(s) 150
TaaI ACNGT 2 cut(s) 291, 327
TaqI TCGA 3 cut(s) 41, 451, 567
TasI AATT 6 cut(s) 95, 120, 170, 198, 296, 703
TatI WGTACW 2 cut(s) 570, 718
TauI GCSGC 1 cut(s) 448
TfiI GAWTC 3 cut(s) 46, 52, 592
TscAI CASTG 1 cut(s) 133
TseFI GTSAC 1 cut(s) 124
TseI GCWGC 2 cut(s) 385, 401
Tsp45I GTSAC 1 cut(s) 124
TspDTI ATGAA 3 cut(s) 49, 98, 259
TspGWI ACGGA 1 cut(s) 626
TspRI CASTG 1 cut(s) 133
Van91I CCANNNNNTGG 1 cut(s) 33
VneI GTGCAC 1 cut(s) 518
VpaK11BI GGWCC 1 cut(s) 21
XmnI GAANNNNTTC 1 cut(s) 56
ZrmI AGTACT 1 cut(s) 572
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.