FvH4_6g20920

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0013255
Physical Location & Seq
Reverse (-)
14413693 .. 14414229
537 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g20920.t1

Sequence Viewer

Length: 537 bp
ATGTCGTCATCTTCCCGGCACCTCTTCCTCCGGCAAGGCTCCTGGAGCCGCCGGCGTCCTCTGCTCAAGACCCACGTCTCCAACTGCAAGGACCGGTTGGCCGAAGTGGCCGGCGGGACCGCCGCCGAGTGCGCCGCCGTGTGCTGCTGCTGCCCTTGCGGGCTGGTCAATCTCCTCGTGCTCGTAATCTACAAGGTTCCAGCGGGGCTCTGCCGCCGCGTGCTGAGAAAGAAGCGACGGCAGAGACTCATCAAGAAAGGGCTGCTCCAGCCGAGCCACCTGCATTGCACGTGCGGCTGCGACGGCCGGGAGCTGCAGTTCCACCCGGTCGGGTTCGAGTCGCTGTCGTCGGAGTTCAAGAAGTATATGATGGAGGACGACGAGTCGTCTGACCAGGATGTGATTCAGCTCGAGAAGGAGATGTGGGATCGGTTTTACGGCACCGGGTTTTGGAGAAGCCCAACTCAGAGAGAAAATCCCTCCATGTCCAACTCCTCCTCCGACTCCTCCGACTCCAAGGTCGTCACCGGTACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

179

Amino Acids

20.07

Weight (kDa)

9.02

Isoelectric Point (pI)

71.21

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013255)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G11690 AT5G06380
fragaria_vesca FvH4_6g20920
malus_domestica MD12G1045100.v1.1 MD14G1044000.v1.1
prunus_persica Prupe.7G090300_v2.0.a1
pyrus_communis pycom14g03730
rosa_chinensis RchiOBHm_Chr3g0477261
rosa_laevigata RLG00000023732
rosa_multiflora Rmu_co8078510.1_g000001 Rmu_sc0000348.1_g000009
rosa_roxburghii Rroxscaffold_6G00404280 Rroxscaffold_6G00404760
rosa_rugosa Rorug03G0159400
rosa_samantha Rh3AG209700 Rh3BG243400
rosa_wichuraiana Rw0G000260 Rw0G021730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 288
AasI GACNNNNNNGTC 1 cut(s) 518
Acc36I ACCTGC 1 cut(s) 288
AccB1I GGYRCC 2 cut(s) 18, 440
AccII CGCG 1 cut(s) 219
AclWI GGATC 1 cut(s) 435
AcoI YGGCCR 3 cut(s) 99, 108, 304
AcvI CACGTG 1 cut(s) 291
AcyI GRCGYC 1 cut(s) 55
AfaI GTAC 1 cut(s) 532
AfiI CCNNNNNNNGG 1 cut(s) 450
AgeI ACCGGT 2 cut(s) 93, 527
AgsI TTSAA 1 cut(s) 358
AhdI GACNNNNNGTC 2 cut(s) 382, 385
AjiI CACGTC 1 cut(s) 76
AjnI CCWGG 2 cut(s) 41, 393
AloI GAACNNNNNNTCC 2 cut(s) 302, 334
AluBI AGCT 2 cut(s) 313, 409
AluI AGCT 2 cut(s) 313, 409
Alw21I GWGCWC 1 cut(s) 183
Alw26I GTCTC 2 cut(s) 82, 238
AlwI GGATC 1 cut(s) 435
Ama87I CYCGRG 1 cut(s) 410
AoxI GGCC 3 cut(s) 99, 108, 304
ApeKI GCWGC 6 cut(s) 144, 147, 150, 262, 297, 313
AsiGI ACCGGT 2 cut(s) 93, 527
AspLEI GCGC 1 cut(s) 134
AspS9I GGNCC 2 cut(s) 91, 117
AsuC2I CCSGG 4 cut(s) 16, 308, 326, 445
AsuHPI GGTGA 1 cut(s) 517
AvaI CYCGRG 1 cut(s) 410
AvaII GGWCC 2 cut(s) 91, 117
BanI GGYRCC 2 cut(s) 18, 440
BanII GRGCYC 1 cut(s) 210
BauI CACGAG 1 cut(s) 176
BbrPI CACGTG 1 cut(s) 291
Bbv12I GWGCWC 1 cut(s) 183
BbvI GCAGC 6 cut(s) 131, 134, 137, 249, 284, 300
BccI CCATC 1 cut(s) 364
BceAI ACGGC 4 cut(s) 122, 254, 319, 454
BcgI CGANNNNNNTGC 2 cut(s) 262, 296
BciT130I CCWGG 2 cut(s) 43, 395
BcnI CCSGG 4 cut(s) 16, 308, 326, 445
BcoDI GTCTC 2 cut(s) 82, 238
BfmI CTRYAG 1 cut(s) 314
BfuAI ACCTGC 1 cut(s) 288
BglI GCCNNNNNGGC 1 cut(s) 107
Bme1390I CCNGG 6 cut(s) 16, 43, 308, 326, 395, 445
Bme18I GGWCC 2 cut(s) 91, 117
BmeRI GACNNNNNGTC 2 cut(s) 382, 385
BmeT110I CYCGRG 1 cut(s) 410
BmgBI CACGTC 1 cut(s) 76
BmgT120I GGNCC 2 cut(s) 91, 117
BmiI GGNNCC 6 cut(s) 20, 40, 47, 118, 198, 442
BmrFI CCNGG 6 cut(s) 16, 43, 308, 326, 395, 445
BoxI GACNNNNGTC 1 cut(s) 74
BpmI CTGGAG 2 cut(s) 64, 251
BpuEI CTTGAG 1 cut(s) 50
BpuMI CCSGG 4 cut(s) 16, 308, 326, 445
BsaAI YACGTR 1 cut(s) 291
BsaHI GRCGYC 1 cut(s) 55
BsaJI CCNNGG 1 cut(s) 516
BsaWI WCCGGW 2 cut(s) 93, 527
BsaXI ACNNNNNCTCC 4 cut(s) 302, 332, 482, 512
Bsc4I CCNNNNNNNGG 1 cut(s) 450
Bse118I RCCGGY 4 cut(s) 51, 93, 110, 527
Bse3DI GCAATG 1 cut(s) 283
BseBI CCWGG 2 cut(s) 43, 395
BseDI CCNNGG 1 cut(s) 516
BseGI GGATG 1 cut(s) 403
BseLI CCNNNNNNNGG 1 cut(s) 450
BseMI GCAATG 1 cut(s) 283
BseMII CTCAG 2 cut(s) 215, 479
BseRI GAGGAG 4 cut(s) 164, 484, 487, 496
BseX3I CGGCCG 1 cut(s) 304
BseXI GCAGC 6 cut(s) 131, 134, 137, 249, 284, 300
Bsh1236I CGCG 1 cut(s) 219
Bsh1285I CGRYCG 2 cut(s) 307, 330
BshFI GGCC 3 cut(s) 101, 110, 306
BshNI GGYRCC 2 cut(s) 18, 440
BshTI ACCGGT 2 cut(s) 93, 527
BsiEI CGRYCG 2 cut(s) 307, 330
BsiHKAI GWGCWC 1 cut(s) 183
BsiHKCI CYCGRG 1 cut(s) 410
BsiSI CCGG 9 cut(s) 16, 31, 52, 94, 111, 307, 326, 444, 528
BslFI GGGAC 1 cut(s) 130
BslI CCNNNNNNNGG 1 cut(s) 450
BsmAI GTCTC 2 cut(s) 82, 238
BsmBI CGTCTC 1 cut(s) 82
BsmFI GGGAC 1 cut(s) 130
BsnI GGCC 3 cut(s) 101, 110, 306
BsoBI CYCGRG 1 cut(s) 410
Bsp1286I GDGCHC 2 cut(s) 183, 210
Bsp143I GATC 1 cut(s) 427
BspANI GGCC 3 cut(s) 101, 110, 306
BspCNI CTCAG 2 cut(s) 216, 478
BspFNI CGCG 1 cut(s) 219
BspLI GGNNCC 6 cut(s) 20, 40, 47, 118, 198, 442
BspMAI CTGCAG 1 cut(s) 318
BspMI ACCTGC 1 cut(s) 288
BspPI GGATC 1 cut(s) 435
BspT107I GGYRCC 2 cut(s) 18, 440
BsrDI GCAATG 1 cut(s) 283
BsrFI RCCGGY 4 cut(s) 51, 93, 110, 527
BssAI RCCGGY 4 cut(s) 51, 93, 110, 527
BssECI CCNNGG 1 cut(s) 516
BssMI GATC 1 cut(s) 427
BssNI GRCGYC 1 cut(s) 55
BssSI CACGAG 1 cut(s) 176
BssT1I CCWWGG 1 cut(s) 516
Bst2BI CACGAG 1 cut(s) 176
Bst2UI CCWGG 2 cut(s) 43, 395
Bst6I CTCTTC 1 cut(s) 29
BstACI GRCGYC 1 cut(s) 55
BstBAI YACGTR 1 cut(s) 291
BstC8I GCNNGC 4 cut(s) 53, 112, 161, 221
BstDEI CTNAG 2 cut(s) 224, 465
BstF5I GGATG 1 cut(s) 403
BstFNI CGCG 1 cut(s) 219
BstHHI GCGC 1 cut(s) 134
BstKTI GATC 1 cut(s) 430
BstMAI GTCTC 2 cut(s) 82, 238
BstMBI GATC 1 cut(s) 427
BstMCI CGRYCG 2 cut(s) 307, 330
BstMWI GCNNNNNNNGC 9 cut(s) 45, 61, 107, 131, 150, 156, 268, 294, 303
BstNI CCWGG 2 cut(s) 43, 395
BstPAI GACNNNNGTC 1 cut(s) 74
BstSCI CCNGG 6 cut(s) 14, 41, 306, 324, 393, 443
BstSFI CTRYAG 1 cut(s) 314
BstUI CGCG 1 cut(s) 219
BstV1I GCAGC 6 cut(s) 131, 134, 137, 249, 284, 300
BstZI CGGCCG 1 cut(s) 304
BsuRI GGCC 3 cut(s) 101, 110, 306
BtrI CACGTC 1 cut(s) 76
BtsCI GGATG 1 cut(s) 403
BveI ACCTGC 1 cut(s) 288
Cac8I GCNNGC 4 cut(s) 53, 112, 161, 221
CfoI GCGC 1 cut(s) 134
Cfr10I RCCGGY 4 cut(s) 51, 93, 110, 527
Cfr13I GGNCC 2 cut(s) 91, 117
CseI GACGC 1 cut(s) 44
Csp6I GTAC 1 cut(s) 531
CspAI ACCGGT 2 cut(s) 93, 527
CspCI CAANNNNNGTGG 2 cut(s) 266, 301
CviAII CATG 1 cut(s) 484
CviQI GTAC 1 cut(s) 531
DdeI CTNAG 2 cut(s) 224, 465
DpnI GATC 1 cut(s) 429
DpnII GATC 1 cut(s) 427
DrdI GACNNNNNNGTC 1 cut(s) 518
DriI GACNNNNNGTC 2 cut(s) 382, 385
DseDI GACNNNNNNGTC 1 cut(s) 518
EaeI YGGCCR 3 cut(s) 99, 108, 304
EagI CGGCCG 1 cut(s) 304
Eam1104I CTCTTC 1 cut(s) 29
Eam1105I GACNNNNNGTC 2 cut(s) 382, 385
EarI CTCTTC 1 cut(s) 29
EclXI CGGCCG 1 cut(s) 304
Eco130I CCWWGG 1 cut(s) 516
Eco24I GRGCYC 1 cut(s) 210
Eco47I GGWCC 2 cut(s) 91, 117
Eco52I CGGCCG 1 cut(s) 304
Eco72I CACGTG 1 cut(s) 291
Eco88I CYCGRG 1 cut(s) 410
EcoRII CCWGG 2 cut(s) 41, 393
EcoT14I CCWWGG 1 cut(s) 516
EcoT38I GRGCYC 1 cut(s) 210
ErhI CCWWGG 1 cut(s) 516
Esp3I CGTCTC 1 cut(s) 82
FaeI CATG 1 cut(s) 487
FaiI YATR 4 cut(s) 366, 368, 485, 535
FaqI GGGAC 1 cut(s) 130
FatI CATG 1 cut(s) 483
FauI CCCGC 3 cut(s) 107, 152, 196
FokI GGATG 1 cut(s) 410
FriOI GRGCYC 1 cut(s) 210
GlaI GCGC 1 cut(s) 133
GsuI CTGGAG 2 cut(s) 64, 251
HaeIII GGCC 3 cut(s) 101, 110, 306
HapII CCGG 9 cut(s) 16, 31, 52, 94, 111, 307, 326, 444, 528
HgaI GACGC 1 cut(s) 44
HhaI GCGC 1 cut(s) 134
Hin1I GRCGYC 1 cut(s) 55
Hin1II CATG 1 cut(s) 487
Hin6I GCGC 1 cut(s) 132
HinP1I GCGC 1 cut(s) 132
HinfI GANTC 6 cut(s) 246, 338, 383, 403, 503, 512
HpaII CCGG 9 cut(s) 16, 31, 52, 94, 111, 307, 326, 444, 528
HphI GGTGA 1 cut(s) 517
Hpy188I TCNGA 5 cut(s) 352, 391, 468, 502, 511
Hpy188III TCNNGA 4 cut(s) 67, 253, 358, 412
Hpy99I CGWCG 4 cut(s) 240, 305, 352, 383
HpyAV CCTTC 1 cut(s) 409
HpyCH4IV ACGT 2 cut(s) 75, 290
HpyCH4V TGCA 4 cut(s) 87, 283, 288, 316
HpyF10VI GCNNNNNNNGC 9 cut(s) 45, 61, 107, 131, 150, 156, 268, 294, 303
HpyF3I CTNAG 2 cut(s) 224, 465
HpySE526I ACGT 2 cut(s) 75, 290
Hsp92I GRCGYC 1 cut(s) 55
Hsp92II CATG 1 cut(s) 487
HspAI GCGC 1 cut(s) 132
KroI GCCGGC 2 cut(s) 51, 110
KroNI GCCGGC 2 cut(s) 53, 112
Kzo9I GATC 1 cut(s) 427
LmnI GCTCC 4 cut(s) 44, 45, 270, 310
Lsp1109I GCAGC 6 cut(s) 131, 134, 137, 249, 284, 300
MaeII ACGT 2 cut(s) 75, 290
MaeIII GTNAC 1 cut(s) 523
MalI GATC 1 cut(s) 429
MboI GATC 1 cut(s) 427
MboII GAAGA 2 cut(s) 3, 16
MhlI GDGCHC 2 cut(s) 183, 210
MlyI GAGTC 5 cut(s) 240, 347, 392, 497, 506
MmeI TCCRAC 5 cut(s) 105, 330, 513, 525, 534
MnlI CCTC 9 cut(s) 32, 38, 69, 185, 367, 490, 505, 508, 517
MreI CGCCGGCG 1 cut(s) 51
MroNI GCCGGC 2 cut(s) 51, 110
MspA1I CMGCKG 1 cut(s) 203
MspI CCGG 9 cut(s) 16, 31, 52, 94, 111, 307, 326, 444, 528
MspR9I CCNGG 6 cut(s) 16, 43, 308, 326, 395, 445
MvaI CCWGG 2 cut(s) 43, 395
MvnI CGCG 1 cut(s) 219
MwoI GCNNNNNNNGC 9 cut(s) 45, 61, 107, 131, 150, 156, 268, 294, 303
NaeI GCCGGC 2 cut(s) 53, 112
NciI CCSGG 4 cut(s) 16, 308, 326, 445
NdeII GATC 1 cut(s) 427
NgoMIV GCCGGC 2 cut(s) 51, 110
NlaIII CATG 1 cut(s) 487
NlaIV GGNNCC 6 cut(s) 20, 40, 47, 118, 198, 442
NmeAIII GCCGAG 2 cut(s) 151, 297
NmuCI GTSAC 1 cut(s) 523
PaeR7I CTCGAG 1 cut(s) 410
PaqCI CACCTGC 1 cut(s) 288
PdiI GCCGGC 2 cut(s) 53, 112
PfeI GAWTC 1 cut(s) 403
PfoI TCCNGGA 1 cut(s) 41
PinAI ACCGGT 2 cut(s) 93, 527
PleI GAGTC 5 cut(s) 240, 346, 391, 497, 506
PmaCI CACGTG 1 cut(s) 291
PmlI CACGTG 1 cut(s) 291
PpsI GAGTC 5 cut(s) 240, 346, 391, 497, 506
Ppu21I YACGTR 1 cut(s) 291
PshAI GACNNNNGTC 1 cut(s) 74
Psp6I CCWGG 2 cut(s) 41, 393
PspCI CACGTG 1 cut(s) 291
PspGI CCWGG 2 cut(s) 41, 393
PspN4I GGNNCC 6 cut(s) 20, 40, 47, 118, 198, 442
PspPI GGNCC 2 cut(s) 91, 117
PstI CTGCAG 1 cut(s) 318
RsaI GTAC 1 cut(s) 532
RsaNI GTAC 1 cut(s) 531
Sau3AI GATC 1 cut(s) 427
Sau96I GGNCC 2 cut(s) 91, 117
SchI GAGTC 5 cut(s) 240, 347, 392, 497, 506
ScrFI CCNGG 6 cut(s) 16, 43, 308, 326, 395, 445
SduI GDGCHC 2 cut(s) 183, 210
SetI ASST 8 cut(s) 24, 78, 198, 282, 293, 315, 411, 522
SfcI CTRYAG 1 cut(s) 314
SfiI GGCCNNNNNGGCC 1 cut(s) 107
Sfr274I CTCGAG 1 cut(s) 410
SgrAI CRCCGGYG 1 cut(s) 51
SinI GGWCC 2 cut(s) 91, 117
SlaI CTCGAG 1 cut(s) 410
SmlI CTYRAG 2 cut(s) 65, 410
SmoI CTYRAG 2 cut(s) 65, 410
StyD4I CCNGG 6 cut(s) 14, 41, 306, 324, 393, 443
StyI CCWWGG 1 cut(s) 516
TaiI ACGT 2 cut(s) 78, 293
TaqI TCGA 2 cut(s) 336, 411
TauI GCSGC 6 cut(s) 51, 125, 137, 216, 219, 297
TfiI GAWTC 1 cut(s) 403
TseFI GTSAC 1 cut(s) 523
TseI GCWGC 6 cut(s) 144, 147, 150, 262, 297, 313
Tsp45I GTSAC 1 cut(s) 523
VpaK11BI GGWCC 2 cut(s) 91, 117
XhoI CTCGAG 1 cut(s) 410
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.