MD12G1045100.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0013255
Physical Location & Seq
Reverse (-)
5031493 .. 5032036
544 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1045100.v1.1.491

Sequence Viewer

Length: 504 bp
ATGTCAGCATCGATAACCTCATCGCGGCAGCTATTTCTCCGGCAGACTTCGTCCCCGCGCCGGCAACCGCTGCTCCGGACCCAAGTCTCAACCAGCAGCGCCCGATTGGCTGAAGTCGCAGGCGGCACAGCCGCCGATTGCGCCGCCGTGTGCTGTTGCTGCCCGTGCGGCCTCGCCAACCTCCTAGTCCTCGTGATCTATAAGGTCCCGGCGGGGCTCTGCCGCCGCGTTCTGAAGAAGAGGCGCCGCCAGAGGCTCATCAAGAAGGGGCTGCAACATCGCAAGTGCAGTTGCGGATTCGCCGGCTCGGAGCTCCAGTTTGACACGGTGGGTTTGGAGTGCGTGGCGGGGATCAACGACATGTCGCAGAAGATTTCCGACGACGAGTCGTATGATGAGGATGTGATGCAGCTCGAGAAGGAGATGTGGGACCGGTTTTACAGTACGGGGTTTTGGCGAAGCCCATCCCAGAGGGAACCGTCCAAGGTTCTCACTGGTAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

168

Amino Acids

18.57

Weight (kDa)

9.32

Isoelectric Point (pI)

66.35

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013255)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G11690 AT5G06380
fragaria_vesca FvH4_6g20920
malus_domestica MD12G1045100.v1.1 MD14G1044000.v1.1
prunus_persica Prupe.7G090300_v2.0.a1
pyrus_communis pycom14g03730
rosa_chinensis RchiOBHm_Chr3g0477261
rosa_laevigata RLG00000023732
rosa_multiflora Rmu_co8078510.1_g000001 Rmu_sc0000348.1_g000009
rosa_roxburghii Rroxscaffold_6G00404280 Rroxscaffold_6G00404760
rosa_rugosa Rorug03G0159400
rosa_samantha Rh3AG209700 Rh3BG243400
rosa_wichuraiana Rw0G000260 Rw0G021730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 243
AccII CGCG 3 cut(s) 25, 58, 228
AccIII TCCGGA 1 cut(s) 75
AclWI GGATC 1 cut(s) 359
AcuI CTGAAG 2 cut(s) 132, 254
AcyI GRCGYC 1 cut(s) 244
AfaI GTAC 1 cut(s) 445
AfiI CCNNNNNNNGG 2 cut(s) 24, 60
AflIII ACRYGT 1 cut(s) 360
AgeI ACCGGT 1 cut(s) 432
AhdI GACNNNNNGTC 1 cut(s) 385
AluBI AGCT 3 cut(s) 31, 313, 412
AluI AGCT 3 cut(s) 31, 313, 412
Alw21I GWGCWC 1 cut(s) 315
Alw26I GTCTC 1 cut(s) 91
AlwI GGATC 1 cut(s) 359
Ama87I CYCGRG 1 cut(s) 413
Aor13HI TCCGGA 1 cut(s) 75
AoxI GGCC 1 cut(s) 169
ApeKI GCWGC 6 cut(s) 28, 70, 96, 159, 271, 409
AsiGI ACCGGT 1 cut(s) 432
AspLEI GCGC 4 cut(s) 60, 101, 143, 246
AspS9I GGNCC 3 cut(s) 78, 205, 430
AsuC2I CCSGG 1 cut(s) 209
AvaI CYCGRG 1 cut(s) 413
AvaII GGWCC 3 cut(s) 78, 205, 430
BanI GGYRCC 1 cut(s) 243
BanII GRGCYC 2 cut(s) 219, 315
BauI CACGAG 1 cut(s) 191
Bbv12I GWGCWC 1 cut(s) 315
BbvI GCAGC 6 cut(s) 40, 57, 108, 146, 258, 421
BccI CCATC 1 cut(s) 472
BceAI ACGGC 1 cut(s) 131
BcnI CCSGG 1 cut(s) 209
BcoDI GTCTC 1 cut(s) 91
BfaI CTAG 1 cut(s) 185
BfoI RGCGCY 2 cut(s) 102, 247
BglI GCCNNNNNGGC 2 cut(s) 107, 168
Bme1390I CCNGG 1 cut(s) 209
Bme18I GGWCC 3 cut(s) 78, 205, 430
BmeRI GACNNNNNGTC 1 cut(s) 385
BmeT110I CYCGRG 1 cut(s) 413
BmgT120I GGNCC 3 cut(s) 78, 205, 430
BmiI GGNNCC 5 cut(s) 80, 207, 245, 431, 477
BmrFI CCNGG 1 cut(s) 209
BmsI GCATC 2 cut(s) 17, 396
BoxI GACNNNNGTC 1 cut(s) 83
BpmI CTGGAG 1 cut(s) 299
BpuMI CCSGG 1 cut(s) 209
Bsa29I ATCGAT 1 cut(s) 11
BsaHI GRCGYC 1 cut(s) 244
BsaJI CCNNGG 1 cut(s) 483
BsaWI WCCGGW 2 cut(s) 75, 432
BsaXI ACNNNNNCTCC 4 cut(s) 57, 87, 302, 332
Bsc4I CCNNNNNNNGG 2 cut(s) 24, 60
Bse118I RCCGGY 3 cut(s) 60, 302, 432
Bse1I ACTGG 2 cut(s) 316, 499
BseAI TCCGGA 1 cut(s) 75
BseCI ATCGAT 1 cut(s) 11
BseDI CCNNGG 1 cut(s) 483
BseGI GGATG 2 cut(s) 406, 464
BseLI CCNNNNNNNGG 2 cut(s) 24, 60
BseNI ACTGG 2 cut(s) 316, 499
BseXI GCAGC 6 cut(s) 40, 57, 108, 146, 258, 421
BsgI GTGCAG 1 cut(s) 307
Bsh1236I CGCG 3 cut(s) 25, 58, 228
BshFI GGCC 1 cut(s) 171
BshNI GGYRCC 1 cut(s) 243
BshTI ACCGGT 1 cut(s) 432
BshVI ATCGAT 1 cut(s) 11
BsiHKAI GWGCWC 1 cut(s) 315
BsiHKCI CYCGRG 1 cut(s) 413
BsiSI CCGG 6 cut(s) 40, 61, 76, 209, 303, 433
BslFI GGGAC 3 cut(s) 37, 191, 443
BslI CCNNNNNNNGG 2 cut(s) 24, 60
BsmAI GTCTC 1 cut(s) 91
BsmFI GGGAC 3 cut(s) 37, 191, 443
BsnI GGCC 1 cut(s) 171
BsoBI CYCGRG 1 cut(s) 413
Bsp1286I GDGCHC 2 cut(s) 219, 315
Bsp13I TCCGGA 1 cut(s) 75
Bsp143I GATC 2 cut(s) 195, 351
BspANI GGCC 1 cut(s) 171
BspDI ATCGAT 1 cut(s) 11
BspEI TCCGGA 1 cut(s) 75
BspFNI CGCG 3 cut(s) 25, 58, 228
BspLI GGNNCC 5 cut(s) 80, 207, 245, 431, 477
BspPI GGATC 1 cut(s) 359
BspT107I GGYRCC 1 cut(s) 243
BsrFI RCCGGY 3 cut(s) 60, 302, 432
BsrI ACTGG 2 cut(s) 316, 499
BssAI RCCGGY 3 cut(s) 60, 302, 432
BssECI CCNNGG 1 cut(s) 483
BssMI GATC 2 cut(s) 195, 351
BssNI GRCGYC 1 cut(s) 244
BssSI CACGAG 1 cut(s) 191
BssT1I CCWWGG 1 cut(s) 483
Bst2BI CACGAG 1 cut(s) 191
Bst4CI ACNGT 3 cut(s) 328, 443, 480
Bst6I CTCTTC 1 cut(s) 233
BstACI GRCGYC 1 cut(s) 244
BstAPI GCANNNNNTGC 1 cut(s) 70
BstC8I GCNNGC 3 cut(s) 62, 121, 304
BstF5I GGATG 2 cut(s) 406, 464
BstFNI CGCG 3 cut(s) 25, 58, 228
BstH2I RGCGCY 2 cut(s) 102, 247
BstHHI GCGC 4 cut(s) 60, 101, 143, 246
BstKTI GATC 2 cut(s) 198, 354
BstMAI GTCTC 1 cut(s) 91
BstMBI GATC 2 cut(s) 195, 351
BstMWI GCNNNNNNNGC 7 cut(s) 70, 107, 116, 140, 159, 165, 168
BstNSI RCATGY 1 cut(s) 364
BstPAI GACNNNNGTC 1 cut(s) 83
BstSCI CCNGG 1 cut(s) 207
BstUI CGCG 3 cut(s) 25, 58, 228
BstV1I GCAGC 6 cut(s) 40, 57, 108, 146, 258, 421
Bsu15I ATCGAT 1 cut(s) 11
BsuRI GGCC 1 cut(s) 171
BsuTUI ATCGAT 1 cut(s) 11
BtgZI GCGATG 2 cut(s) 6, 263
BtsCI GGATG 2 cut(s) 406, 464
BtsIMutI CAGTG 1 cut(s) 492
Cac8I GCNNGC 3 cut(s) 62, 121, 304
CfoI GCGC 4 cut(s) 60, 101, 143, 246
Cfr10I RCCGGY 3 cut(s) 60, 302, 432
Cfr13I GGNCC 3 cut(s) 78, 205, 430
ClaI ATCGAT 1 cut(s) 11
Csp6I GTAC 1 cut(s) 444
CspAI ACCGGT 1 cut(s) 432
CviAII CATG 1 cut(s) 361
CviQI GTAC 1 cut(s) 444
DinI GGCGCC 1 cut(s) 245
DpnI GATC 2 cut(s) 197, 353
DpnII GATC 2 cut(s) 195, 351
DriI GACNNNNNGTC 1 cut(s) 385
Eam1104I CTCTTC 1 cut(s) 233
Eam1105I GACNNNNNGTC 1 cut(s) 385
EarI CTCTTC 1 cut(s) 233
Ecl136II GAGCTC 1 cut(s) 313
Eco130I CCWWGG 1 cut(s) 483
Eco24I GRGCYC 2 cut(s) 219, 315
Eco47I GGWCC 3 cut(s) 78, 205, 430
Eco53kI GAGCTC 1 cut(s) 313
Eco57I CTGAAG 2 cut(s) 132, 254
Eco88I CYCGRG 1 cut(s) 413
EcoICRI GAGCTC 1 cut(s) 313
EcoO109I RGGNCCY 1 cut(s) 205
EcoT14I CCWWGG 1 cut(s) 483
EcoT38I GRGCYC 2 cut(s) 219, 315
EgeI GGCGCC 1 cut(s) 245
EheI GGCGCC 1 cut(s) 245
ErhI CCWWGG 1 cut(s) 483
FaeI CATG 1 cut(s) 364
FaiI YATR 3 cut(s) 201, 362, 393
FaqI GGGAC 3 cut(s) 37, 191, 443
FatI CATG 1 cut(s) 360
FauI CCCGC 3 cut(s) 63, 205, 340
FokI GGATG 2 cut(s) 413, 451
FriOI GRGCYC 2 cut(s) 219, 315
FspBI CTAG 1 cut(s) 185
GlaI GCGC 4 cut(s) 59, 100, 142, 245
GsuI CTGGAG 1 cut(s) 299
HaeII RGCGCY 2 cut(s) 102, 247
HaeIII GGCC 1 cut(s) 171
HapII CCGG 6 cut(s) 40, 61, 76, 209, 303, 433
HhaI GCGC 4 cut(s) 60, 101, 143, 246
Hin1I GRCGYC 1 cut(s) 244
Hin1II CATG 1 cut(s) 364
Hin6I GCGC 4 cut(s) 58, 99, 141, 244
HinP1I GCGC 4 cut(s) 58, 99, 141, 244
HinfI GANTC 2 cut(s) 297, 386
HpaII CCGG 6 cut(s) 40, 61, 76, 209, 303, 433
Hpy188I TCNGA 3 cut(s) 234, 310, 379
Hpy188III TCNNGA 4 cut(s) 76, 193, 262, 415
Hpy99I CGWCG 2 cut(s) 383, 386
HpyAV CCTTC 2 cut(s) 259, 412
HpyCH4III ACNGT 3 cut(s) 328, 443, 480
HpyCH4V TGCA 3 cut(s) 274, 288, 409
HpyF10VI GCNNNNNNNGC 7 cut(s) 70, 107, 116, 140, 159, 165, 168
Hsp92I GRCGYC 1 cut(s) 244
Hsp92II CATG 1 cut(s) 364
HspAI GCGC 4 cut(s) 58, 99, 141, 244
KasI GGCGCC 1 cut(s) 243
Kpn2I TCCGGA 1 cut(s) 75
KroI GCCGGC 2 cut(s) 60, 302
KroNI GCCGGC 2 cut(s) 62, 304
Kzo9I GATC 2 cut(s) 195, 351
LmnI GCTCC 3 cut(s) 78, 310, 318
Lsp1109I GCAGC 6 cut(s) 40, 57, 108, 146, 258, 421
LweI GCATC 2 cut(s) 17, 396
MaeI CTAG 1 cut(s) 185
MalI GATC 2 cut(s) 197, 353
MboI GATC 2 cut(s) 195, 351
MboII GAAGA 3 cut(s) 247, 250, 382
MhlI GDGCHC 2 cut(s) 219, 315
Mly113I GGCGCC 1 cut(s) 244
MlyI GAGTC 1 cut(s) 395
MmeI TCCRAC 1 cut(s) 402
MnlI CCTC 8 cut(s) 28, 182, 191, 200, 234, 246, 391, 465
MroI TCCGGA 1 cut(s) 75
MroNI GCCGGC 2 cut(s) 60, 302
MspA1I CMGCKG 1 cut(s) 70
MspI CCGG 6 cut(s) 40, 61, 76, 209, 303, 433
MspR9I CCNGG 1 cut(s) 209
MvnI CGCG 3 cut(s) 25, 58, 228
MwoI GCNNNNNNNGC 7 cut(s) 70, 107, 116, 140, 159, 165, 168
NaeI GCCGGC 2 cut(s) 62, 304
NarI GGCGCC 1 cut(s) 244
NciI CCSGG 1 cut(s) 209
NdeII GATC 2 cut(s) 195, 351
NgoMIV GCCGGC 2 cut(s) 60, 302
NlaIII CATG 1 cut(s) 364
NlaIV GGNNCC 5 cut(s) 80, 207, 245, 431, 477
NspI RCATGY 1 cut(s) 364
PaeR7I CTCGAG 1 cut(s) 413
PciI ACATGT 1 cut(s) 360
PdiI GCCGGC 2 cut(s) 62, 304
PfeI GAWTC 1 cut(s) 297
PflFI GACNNNGTC 1 cut(s) 49
PinAI ACCGGT 1 cut(s) 432
PleI GAGTC 1 cut(s) 394
PluTI GGCGCC 1 cut(s) 247
PpsI GAGTC 1 cut(s) 394
PpuMI RGGWCCY 1 cut(s) 205
PscI ACATGT 1 cut(s) 360
PshAI GACNNNNGTC 1 cut(s) 83
Psp124BI GAGCTC 1 cut(s) 315
Psp5II RGGWCCY 1 cut(s) 205
PspN4I GGNNCC 5 cut(s) 80, 207, 245, 431, 477
PspPI GGNCC 3 cut(s) 78, 205, 430
PspPPI RGGWCCY 1 cut(s) 205
PsyI GACNNNGTC 1 cut(s) 49
RsaI GTAC 1 cut(s) 445
RsaNI GTAC 1 cut(s) 444
SacI GAGCTC 1 cut(s) 315
Sau3AI GATC 2 cut(s) 195, 351
Sau96I GGNCC 3 cut(s) 78, 205, 430
SchI GAGTC 1 cut(s) 395
ScrFI CCNGG 1 cut(s) 209
SduI GDGCHC 2 cut(s) 219, 315
SetI ASST 7 cut(s) 20, 33, 183, 207, 315, 414, 489
SfaNI GCATC 2 cut(s) 17, 396
SfoI GGCGCC 1 cut(s) 245
Sfr274I CTCGAG 1 cut(s) 413
SinI GGWCC 3 cut(s) 78, 205, 430
SlaI CTCGAG 1 cut(s) 413
SmlI CTYRAG 1 cut(s) 413
SmoI CTYRAG 1 cut(s) 413
SspDI GGCGCC 1 cut(s) 243
SspMI CTAG 1 cut(s) 185
SstI GAGCTC 1 cut(s) 315
StyD4I CCNGG 1 cut(s) 207
StyI CCWWGG 1 cut(s) 483
TaaI ACNGT 3 cut(s) 328, 443, 480
TaqI TCGA 2 cut(s) 11, 414
TauI GCSGC 8 cut(s) 28, 126, 134, 146, 171, 225, 228, 249
TfiI GAWTC 1 cut(s) 297
TscAI CASTG 1 cut(s) 499
TseI GCWGC 6 cut(s) 28, 70, 96, 159, 271, 409
TspRI CASTG 1 cut(s) 499
Tth111I GACNNNGTC 1 cut(s) 49
VpaK11BI GGWCC 3 cut(s) 78, 205, 430
XceI RCATGY 1 cut(s) 364
XhoI CTCGAG 1 cut(s) 413
XspI CTAG 1 cut(s) 185
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.