RLG00000023732

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0013255
Physical Location & Seq
Forward (+)
27609083 .. 27610613
1531 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000023732

Sequence Viewer

Length: 543 bp
ATGTCGTCCCGGCACGTCTTCCTCCGGCAAGCCTCCTCCGGGAACCGCCGGCGGCCTCTGCTCAAAACCCACGTCAGCGACTGCAAGGACCGGGTGGCCGAAGTCGCCGGCGGGACCGCCGCCGAGTGCGCCGCCGTGTGTTGCTGCTGCCCATGCGGGCTGGTCAATCTGCTAGTCCTGGTAATCTACAAGGTCCCGGTCAGGCTCTGCCGCCGCGTCATGAGGAAGAACCGCCGGCAGAGGCTCATCAAGAAAGGGCTTCTTCCGCCGCGCCACGTGCAATGCACGTGCGGCTGCGACGGCCTGGAGCTGCAGTTCCACCCGGTCGGGTTCGAGTCGCTGACGTCGGAGTTCAAGGCGGTGCAGAAGTTGGTGATGGAAGAGGAGTCGTCTGACGAGGATGTGATTCAGCTCGAGAAGGAGATGTGGGATCGGTTTTACGGCACTGGGTTTTGGAGAAGCCCAACTCAGAGAGAAACTCCCTCCATCTCCAACTCCAACTCCAACTCCAACTCAAACTCCAAGATGATCACCGGCCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

181

Amino Acids

20.29

Weight (kDa)

9.16

Isoelectric Point (pI)

57.52

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0013255)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G11690 AT5G06380
fragaria_vesca FvH4_6g20920
malus_domestica MD12G1045100.v1.1 MD14G1044000.v1.1
prunus_persica Prupe.7G090300_v2.0.a1
pyrus_communis pycom14g03730
rosa_chinensis RchiOBHm_Chr3g0477261
rosa_laevigata RLG00000023732
rosa_multiflora Rmu_co8078510.1_g000001 Rmu_sc0000348.1_g000009
rosa_roxburghii Rroxscaffold_6G00404280 Rroxscaffold_6G00404760
rosa_rugosa Rorug03G0159400
rosa_samantha Rh3AG209700 Rh3BG243400
rosa_wichuraiana Rw0G000260 Rw0G021730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 347
AccII CGCG 2 cut(s) 216, 271
AclWI GGATC 1 cut(s) 438
AcoI YGGCCR 2 cut(s) 96, 535
AcvI CACGTG 2 cut(s) 277, 288
AcyI GRCGYC 1 cut(s) 344
AfiI CCNNNNNNNGG 1 cut(s) 39
AgsI TTSAA 1 cut(s) 355
AjiI CACGTC 2 cut(s) 16, 73
AjnI CCWGG 2 cut(s) 177, 303
AloI GAACNNNNNNTCC 2 cut(s) 299, 331
AluBI AGCT 2 cut(s) 310, 412
AluI AGCT 2 cut(s) 310, 412
AlwI GGATC 1 cut(s) 438
AlwNI CAGNNNCTG 2 cut(s) 81, 207
Ama87I CYCGRG 1 cut(s) 413
AoxI GGCC 4 cut(s) 53, 96, 301, 535
ApeKI GCWGC 4 cut(s) 144, 147, 294, 310
ArsI GACNNNNNNTTYG 2 cut(s) 57, 89
AspLEI GCGC 2 cut(s) 131, 273
AspS9I GGNCC 3 cut(s) 88, 114, 193
AsuC2I CCSGG 5 cut(s) 10, 40, 92, 197, 323
AsuHPI GGTGA 2 cut(s) 385, 523
AvaI CYCGRG 1 cut(s) 413
AvaII GGWCC 3 cut(s) 88, 114, 193
BbrPI CACGTG 2 cut(s) 277, 288
BbsI GAAGAC 1 cut(s) 10
BbvI GCAGC 4 cut(s) 131, 134, 281, 297
BccI CCATC 2 cut(s) 370, 494
BceAI ACGGC 3 cut(s) 119, 316, 457
BciT130I CCWGG 2 cut(s) 179, 305
BclI TGATCA 1 cut(s) 528
BcnI CCSGG 5 cut(s) 10, 40, 92, 197, 323
BfaI CTAG 1 cut(s) 173
BfmI CTRYAG 1 cut(s) 311
Bme1390I CCNGG 7 cut(s) 10, 40, 92, 179, 197, 305, 323
Bme18I GGWCC 3 cut(s) 88, 114, 193
BmeT110I CYCGRG 1 cut(s) 413
BmgBI CACGTC 2 cut(s) 16, 73
BmgT120I GGNCC 3 cut(s) 88, 114, 193
BmiI GGNNCC 3 cut(s) 44, 115, 195
BmrFI CCNGG 7 cut(s) 10, 40, 92, 179, 197, 305, 323
BmrI ACTGGG 1 cut(s) 456
BmuI ACTGGG 1 cut(s) 456
BpiI GAAGAC 1 cut(s) 10
BplI GAGNNNNNCTC 2 cut(s) 463, 495
BpmI CTGGAG 1 cut(s) 326
BpuMI CCSGG 5 cut(s) 10, 40, 92, 197, 323
BsaAI YACGTR 2 cut(s) 277, 288
BsaHI GRCGYC 1 cut(s) 344
BsaXI ACNNNNNCTCC 8 cut(s) 299, 329, 485, 491, 503, 515, 521, 533
Bsc4I CCNNNNNNNGG 1 cut(s) 39
Bse118I RCCGGY 4 cut(s) 48, 107, 234, 533
Bse1I ACTGG 1 cut(s) 451
Bse3DI GCAATG 1 cut(s) 287
BseBI CCWGG 2 cut(s) 179, 305
BseGI GGATG 1 cut(s) 406
BseLI CCNNNNNNNGG 1 cut(s) 39
BseMI GCAATG 1 cut(s) 287
BseMII CTCAG 1 cut(s) 482
BseNI ACTGG 1 cut(s) 451
BseRI GAGGAG 2 cut(s) 25, 398
BseXI GCAGC 4 cut(s) 131, 134, 281, 297
BsgI GTGCAG 1 cut(s) 383
Bsh1236I CGCG 2 cut(s) 216, 271
Bsh1285I CGRYCG 1 cut(s) 327
BshFI GGCC 4 cut(s) 55, 98, 303, 537
BsiEI CGRYCG 1 cut(s) 327
BsiHKCI CYCGRG 1 cut(s) 413
BslFI GGGAC 2 cut(s) 127, 179
BslI CCNNNNNNNGG 1 cut(s) 39
BsmFI GGGAC 2 cut(s) 127, 179
BsnI GGCC 4 cut(s) 55, 98, 303, 537
BsoBI CYCGRG 1 cut(s) 413
Bsp143I GATC 2 cut(s) 430, 528
BspANI GGCC 4 cut(s) 55, 98, 303, 537
BspCNI CTCAG 1 cut(s) 481
BspFNI CGCG 2 cut(s) 216, 271
BspHI TCATGA 1 cut(s) 219
BspLI GGNNCC 3 cut(s) 44, 115, 195
BspMAI CTGCAG 1 cut(s) 315
BspPI GGATC 1 cut(s) 438
BsrDI GCAATG 1 cut(s) 287
BsrFI RCCGGY 4 cut(s) 48, 107, 234, 533
BsrI ACTGG 1 cut(s) 451
BssAI RCCGGY 4 cut(s) 48, 107, 234, 533
BssMI GATC 2 cut(s) 430, 528
BssNI GRCGYC 1 cut(s) 344
Bst2UI CCWGG 2 cut(s) 179, 305
Bst6I CTCTTC 1 cut(s) 375
BstACI GRCGYC 1 cut(s) 344
BstBAI YACGTR 2 cut(s) 277, 288
BstC8I GCNNGC 5 cut(s) 30, 50, 109, 158, 236
BstDEI CTNAG 1 cut(s) 468
BstF5I GGATG 1 cut(s) 406
BstFNI CGCG 2 cut(s) 216, 271
BstHHI GCGC 2 cut(s) 131, 273
BstKTI GATC 2 cut(s) 433, 531
BstMBI GATC 2 cut(s) 430, 528
BstMCI CGRYCG 1 cut(s) 327
BstMWI GCNNNNNNNGC 8 cut(s) 58, 104, 128, 153, 265, 277, 291, 300
BstNI CCWGG 2 cut(s) 179, 305
BstSCI CCNGG 7 cut(s) 8, 38, 90, 177, 195, 303, 321
BstSFI CTRYAG 1 cut(s) 311
BstUI CGCG 2 cut(s) 216, 271
BstV1I GCAGC 4 cut(s) 131, 134, 281, 297
BstV2I GAAGAC 1 cut(s) 10
BsuRI GGCC 4 cut(s) 55, 98, 303, 537
BtrI CACGTC 2 cut(s) 16, 73
BtsCI GGATG 1 cut(s) 406
BtsIMutI CAGTG 1 cut(s) 444
Cac8I GCNNGC 5 cut(s) 30, 50, 109, 158, 236
CaiI CAGNNNCTG 2 cut(s) 81, 207
CciI TCATGA 1 cut(s) 219
CfoI GCGC 2 cut(s) 131, 273
Cfr10I RCCGGY 4 cut(s) 48, 107, 234, 533
Cfr13I GGNCC 3 cut(s) 88, 114, 193
CseI GACGC 1 cut(s) 205
CviAII CATG 2 cut(s) 153, 220
DdeI CTNAG 1 cut(s) 468
DpnI GATC 2 cut(s) 432, 530
DpnII GATC 2 cut(s) 430, 528
EaeI YGGCCR 2 cut(s) 96, 535
Eam1104I CTCTTC 1 cut(s) 375
EarI CTCTTC 1 cut(s) 375
EciI GGCGGA 1 cut(s) 255
Eco47I GGWCC 3 cut(s) 88, 114, 193
Eco72I CACGTG 2 cut(s) 277, 288
Eco88I CYCGRG 1 cut(s) 413
EcoO109I RGGNCCY 1 cut(s) 193
EcoRII CCWGG 2 cut(s) 177, 303
FaeI CATG 2 cut(s) 156, 223
FaiI YATR 2 cut(s) 154, 221
FalI AAGNNNNNCTT 2 cut(s) 246, 278
FaqI GGGAC 2 cut(s) 127, 179
FatI CATG 2 cut(s) 152, 219
FauI CCCGC 2 cut(s) 104, 149
FbaI TGATCA 1 cut(s) 528
FokI GGATG 1 cut(s) 413
FspBI CTAG 1 cut(s) 173
GlaI GCGC 2 cut(s) 130, 272
GsuI CTGGAG 1 cut(s) 326
HaeIII GGCC 4 cut(s) 55, 98, 303, 537
HgaI GACGC 1 cut(s) 205
HhaI GCGC 2 cut(s) 131, 273
Hin1I GRCGYC 1 cut(s) 344
Hin1II CATG 2 cut(s) 156, 223
Hin6I GCGC 2 cut(s) 129, 271
HinP1I GCGC 2 cut(s) 129, 271
HinfI GANTC 3 cut(s) 335, 386, 406
HphI GGTGA 2 cut(s) 385, 523
Hpy188I TCNGA 3 cut(s) 349, 394, 471
Hpy188III TCNNGA 3 cut(s) 220, 250, 415
Hpy99I CGWCG 2 cut(s) 302, 349
HpyAV CCTTC 1 cut(s) 412
HpyCH4IV ACGT 5 cut(s) 15, 72, 276, 287, 344
HpyCH4V TGCA 5 cut(s) 84, 280, 285, 313, 364
HpyF10VI GCNNNNNNNGC 8 cut(s) 58, 104, 128, 153, 265, 277, 291, 300
HpyF3I CTNAG 1 cut(s) 468
HpySE526I ACGT 5 cut(s) 15, 72, 276, 287, 344
Hsp92I GRCGYC 1 cut(s) 344
Hsp92II CATG 2 cut(s) 156, 223
HspAI GCGC 2 cut(s) 129, 271
KroI GCCGGC 3 cut(s) 48, 107, 234
KroNI GCCGGC 3 cut(s) 50, 109, 236
Ksp22I TGATCA 1 cut(s) 528
Kzo9I GATC 2 cut(s) 430, 528
LmnI GCTCC 1 cut(s) 307
Lsp1109I GCAGC 4 cut(s) 131, 134, 281, 297
MaeI CTAG 1 cut(s) 173
MaeII ACGT 5 cut(s) 15, 72, 276, 287, 344
MalI GATC 2 cut(s) 432, 530
MboI GATC 2 cut(s) 430, 528
MboII GAAGA 4 cut(s) 10, 238, 254, 392
MlyI GAGTC 2 cut(s) 344, 395
MmeI TCCRAC 5 cut(s) 327, 516, 522, 528, 534
MnlI CCTC 9 cut(s) 32, 43, 46, 66, 216, 234, 376, 391, 493
MreI CGCCGGCG 2 cut(s) 48, 107
MroNI GCCGGC 3 cut(s) 48, 107, 234
MseI TTAA 1 cut(s) 541
MspR9I CCNGG 7 cut(s) 10, 40, 92, 179, 197, 305, 323
MvaI CCWGG 2 cut(s) 179, 305
MvnI CGCG 2 cut(s) 216, 271
MwoI GCNNNNNNNGC 8 cut(s) 58, 104, 128, 153, 265, 277, 291, 300
NaeI GCCGGC 3 cut(s) 50, 109, 236
NciI CCSGG 5 cut(s) 10, 40, 92, 197, 323
NdeII GATC 2 cut(s) 430, 528
NgoMIV GCCGGC 3 cut(s) 48, 107, 234
NlaIII CATG 2 cut(s) 156, 223
NlaIV GGNNCC 3 cut(s) 44, 115, 195
NmeAIII GCCGAG 1 cut(s) 148
PaeR7I CTCGAG 1 cut(s) 413
PagI TCATGA 1 cut(s) 219
PdiI GCCGGC 3 cut(s) 50, 109, 236
PfeI GAWTC 1 cut(s) 406
PfoI TCCNGGA 1 cut(s) 38
PleI GAGTC 2 cut(s) 343, 394
PmaCI CACGTG 2 cut(s) 277, 288
PmlI CACGTG 2 cut(s) 277, 288
PpsI GAGTC 2 cut(s) 343, 394
Ppu21I YACGTR 2 cut(s) 277, 288
PpuMI RGGWCCY 1 cut(s) 193
Psp5II RGGWCCY 1 cut(s) 193
Psp6I CCWGG 2 cut(s) 177, 303
PspCI CACGTG 2 cut(s) 277, 288
PspGI CCWGG 2 cut(s) 177, 303
PspN4I GGNNCC 3 cut(s) 44, 115, 195
PspPI GGNCC 3 cut(s) 88, 114, 193
PspPPI RGGWCCY 1 cut(s) 193
PstI CTGCAG 1 cut(s) 315
PstNI CAGNNNCTG 2 cut(s) 81, 207
SaqAI TTAA 1 cut(s) 541
Sau3AI GATC 2 cut(s) 430, 528
Sau96I GGNCC 3 cut(s) 88, 114, 193
SchI GAGTC 2 cut(s) 344, 395
ScrFI CCNGG 7 cut(s) 10, 40, 92, 179, 197, 305, 323
SetI ASST 8 cut(s) 18, 75, 195, 279, 290, 312, 347, 414
SfcI CTRYAG 1 cut(s) 311
Sfr274I CTCGAG 1 cut(s) 413
SgrAI CRCCGGYG 2 cut(s) 48, 107
SinI GGWCC 3 cut(s) 88, 114, 193
SlaI CTCGAG 1 cut(s) 413
SmlI CTYRAG 1 cut(s) 413
SmoI CTYRAG 1 cut(s) 413
SspMI CTAG 1 cut(s) 173
StyD4I CCNGG 7 cut(s) 8, 38, 90, 177, 195, 303, 321
TaiI ACGT 5 cut(s) 18, 75, 279, 290, 347
TaqI TCGA 2 cut(s) 333, 414
TauI GCSGC 7 cut(s) 55, 122, 134, 213, 216, 271, 294
TfiI GAWTC 1 cut(s) 406
Tru1I TTAA 1 cut(s) 541
Tru9I TTAA 1 cut(s) 541
TscAI CASTG 1 cut(s) 451
TseI GCWGC 4 cut(s) 144, 147, 294, 310
TspRI CASTG 1 cut(s) 451
VpaK11BI GGWCC 3 cut(s) 88, 114, 193
XhoI CTCGAG 1 cut(s) 413
XspI CTAG 1 cut(s) 173
ZraI GACGTC 1 cut(s) 345
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.