pycom14g03730

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0013255
Physical Location & Seq
Reverse (-)
3133915 .. 3134430
516 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g03730.1

Sequence Viewer

Length: 516 bp
ATGTCAGCATCGATAACCTCATCGCGGCAACTGTTTCTCCGGCAGACTTCTTCGCCGCGCCGGCAGCCGCTGCTACGGACCCAAGTCTCAACCAGCACCACCCGATTTGCTGAAGTCGTCGGCGGCACCACCGCCGATTGCGCTGCCGTGTGCTGCTGCTGCCCGTGGGGCCTCGTCAACCTCTTAGTTCTCGCAATCTACAAGGTCCCGGCGGGGCTCTGCCGCCGCGTTCTGAAGAAGAAACACCGCCAGCGGCTGATCAAAAAGGGGCTGTTGCAGCCCCGGAATCGGAAGTGCAGTTGCGGGTTCGCCGGCTCGGAGCTCCAGTTCCACCCGGTGGGTTTGCAGTGCGTGGCGGAGATCAACAACAGGTCGCAGAAGGTCTCCGACGATGAGTCGTGTGACGAGGATGTGATGCAGCTCGAGAAGGAGATGTGGGACCGGTTTTACACTACCGGGTTTTGGAGGAGCCCATCCCAGAGGGAACCGTCGTCCAAGGTTCTAACTGGTACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

172

Amino Acids

19.2

Weight (kDa)

9.55

Isoelectric Point (pI)

59.79

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013255)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G11690 AT5G06380
fragaria_vesca FvH4_6g20920
malus_domestica MD12G1045100.v1.1 MD14G1044000.v1.1
prunus_persica Prupe.7G090300_v2.0.a1
pyrus_communis pycom14g03730
rosa_chinensis RchiOBHm_Chr3g0477261
rosa_laevigata RLG00000023732
rosa_multiflora Rmu_co8078510.1_g000001 Rmu_sc0000348.1_g000009
rosa_roxburghii Rroxscaffold_6G00404280 Rroxscaffold_6G00404760
rosa_rugosa Rorug03G0159400
rosa_samantha Rh3AG209700 Rh3BG243400
rosa_wichuraiana Rw0G000260 Rw0G021730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 125
AccB7I CCANNNNNTGG 1 cut(s) 337
AccII CGCG 3 cut(s) 25, 58, 228
AcuI CTGAAG 2 cut(s) 132, 254
AdeI CACNNNGTG 1 cut(s) 337
AfaI GTAC 1 cut(s) 511
AfiI CCNNNNNNNGG 4 cut(s) 24, 288, 337, 462
AgeI ACCGGT 1 cut(s) 441
AhdI GACNNNNNGTC 1 cut(s) 394
AloI GAACNNNNNNTCC 2 cut(s) 311, 343
AluBI AGCT 2 cut(s) 322, 421
AluI AGCT 2 cut(s) 322, 421
Alw21I GWGCWC 1 cut(s) 324
Alw26I GTCTC 2 cut(s) 91, 388
AlwNI CAGNNNCTG 2 cut(s) 70, 256
Ama87I CYCGRG 1 cut(s) 422
AoxI GGCC 1 cut(s) 169
ApeKI GCWGC 8 cut(s) 64, 70, 143, 153, 156, 159, 277, 418
AsiGI ACCGGT 1 cut(s) 441
AspLEI GCGC 2 cut(s) 60, 143
AspS9I GGNCC 4 cut(s) 78, 169, 205, 439
AsuC2I CCSGG 4 cut(s) 209, 283, 335, 457
AvaI CYCGRG 1 cut(s) 422
AvaII GGWCC 3 cut(s) 78, 205, 439
BanI GGYRCC 1 cut(s) 125
BanII GRGCYC 3 cut(s) 219, 324, 473
Bbv12I GWGCWC 1 cut(s) 324
BbvI GCAGC 8 cut(s) 57, 76, 130, 140, 143, 146, 289, 430
BccI CCATC 1 cut(s) 481
BceAI ACGGC 1 cut(s) 131
BcgI CGANNNNNNTGC 2 cut(s) 125, 159
BclI TGATCA 1 cut(s) 258
BcnI CCSGG 4 cut(s) 209, 283, 335, 457
BcoDI GTCTC 2 cut(s) 91, 388
BglI GCCNNNNNGGC 2 cut(s) 61, 168
Bme1390I CCNGG 4 cut(s) 209, 283, 335, 457
Bme18I GGWCC 3 cut(s) 78, 205, 439
BmeRI GACNNNNNGTC 1 cut(s) 394
BmeT110I CYCGRG 1 cut(s) 422
BmgT120I GGNCC 4 cut(s) 78, 169, 205, 439
BmiI GGNNCC 7 cut(s) 80, 127, 170, 207, 440, 470, 486
BmrFI CCNGG 4 cut(s) 209, 283, 335, 457
BmsI GCATC 2 cut(s) 17, 405
BoxI GACNNNNGTC 1 cut(s) 83
BpmI CTGGAG 1 cut(s) 308
BpuMI CCSGG 4 cut(s) 209, 283, 335, 457
Bsa29I ATCGAT 1 cut(s) 11
BsaI GGTCTC 1 cut(s) 388
BsaJI CCNNGG 3 cut(s) 164, 281, 495
BsaWI WCCGGW 1 cut(s) 441
BsaXI ACNNNNNCTCC 4 cut(s) 21, 51, 311, 341
Bsc4I CCNNNNNNNGG 4 cut(s) 24, 288, 337, 462
Bse118I RCCGGY 3 cut(s) 60, 311, 441
Bse1I ACTGG 2 cut(s) 325, 511
BseCI ATCGAT 1 cut(s) 11
BseDI CCNNGG 3 cut(s) 164, 281, 495
BseGI GGATG 2 cut(s) 415, 473
BseLI CCNNNNNNNGG 4 cut(s) 24, 288, 337, 462
BseNI ACTGG 2 cut(s) 325, 511
BseRI GAGGAG 1 cut(s) 481
BseXI GCAGC 8 cut(s) 57, 76, 130, 140, 143, 146, 289, 430
BsgI GTGCAG 1 cut(s) 316
Bsh1236I CGCG 3 cut(s) 25, 58, 228
BshFI GGCC 1 cut(s) 171
BshNI GGYRCC 1 cut(s) 125
BshTI ACCGGT 1 cut(s) 441
BshVI ATCGAT 1 cut(s) 11
BsiHKAI GWGCWC 1 cut(s) 324
BsiHKCI CYCGRG 1 cut(s) 422
BsiSI CCGG 8 cut(s) 40, 61, 209, 283, 312, 335, 442, 456
BslFI GGGAC 2 cut(s) 191, 452
BslI CCNNNNNNNGG 4 cut(s) 24, 288, 337, 462
BsmAI GTCTC 2 cut(s) 91, 388
BsmFI GGGAC 2 cut(s) 191, 452
BsnI GGCC 1 cut(s) 171
Bso31I GGTCTC 1 cut(s) 388
BsoBI CYCGRG 1 cut(s) 422
Bsp1286I GDGCHC 3 cut(s) 219, 324, 473
Bsp143I GATC 2 cut(s) 258, 360
BspANI GGCC 1 cut(s) 171
BspDI ATCGAT 1 cut(s) 11
BspFNI CGCG 3 cut(s) 25, 58, 228
BspLI GGNNCC 7 cut(s) 80, 127, 170, 207, 440, 470, 486
BspT107I GGYRCC 1 cut(s) 125
BspTNI GGTCTC 1 cut(s) 388
BsrFI RCCGGY 3 cut(s) 60, 311, 441
BsrI ACTGG 2 cut(s) 325, 511
BssAI RCCGGY 3 cut(s) 60, 311, 441
BssECI CCNNGG 3 cut(s) 164, 281, 495
BssMI GATC 2 cut(s) 258, 360
BssT1I CCWWGG 1 cut(s) 495
Bst4CI ACNGT 2 cut(s) 33, 489
BstAPI GCANNNNNTGC 1 cut(s) 70
BstC8I GCNNGC 3 cut(s) 62, 251, 313
BstDEI CTNAG 1 cut(s) 184
BstDSI CCRYGG 1 cut(s) 164
BstF5I GGATG 2 cut(s) 415, 473
BstFNI CGCG 3 cut(s) 25, 58, 228
BstHHI GCGC 2 cut(s) 60, 143
BstKTI GATC 2 cut(s) 261, 363
BstMAI GTCTC 2 cut(s) 91, 388
BstMBI GATC 2 cut(s) 258, 360
BstMWI GCNNNNNNNGC 7 cut(s) 61, 64, 70, 140, 159, 168, 277
BstPAI GACNNNNGTC 1 cut(s) 83
BstSCI CCNGG 4 cut(s) 207, 281, 333, 455
BstUI CGCG 3 cut(s) 25, 58, 228
BstV1I GCAGC 8 cut(s) 57, 76, 130, 140, 143, 146, 289, 430
Bsu15I ATCGAT 1 cut(s) 11
BsuRI GGCC 1 cut(s) 171
BsuTUI ATCGAT 1 cut(s) 11
BtgI CCRYGG 1 cut(s) 164
BtgZI GCGATG 1 cut(s) 6
BtsCI GGATG 2 cut(s) 415, 473
BtsI GCAGTG 1 cut(s) 353
BtsIMutI CAGTG 1 cut(s) 353
Cac8I GCNNGC 3 cut(s) 62, 251, 313
CaiI CAGNNNCTG 2 cut(s) 70, 256
CfoI GCGC 2 cut(s) 60, 143
Cfr10I RCCGGY 3 cut(s) 60, 311, 441
Cfr13I GGNCC 4 cut(s) 78, 169, 205, 439
ClaI ATCGAT 1 cut(s) 11
Csp6I GTAC 1 cut(s) 510
CspAI ACCGGT 1 cut(s) 441
CspCI CAANNNNNGTGG 2 cut(s) 88, 123
CviQI GTAC 1 cut(s) 510
DdeI CTNAG 1 cut(s) 184
DpnI GATC 2 cut(s) 260, 362
DpnII GATC 2 cut(s) 258, 360
DraIII CACNNNGTG 1 cut(s) 337
DriI GACNNNNNGTC 1 cut(s) 394
Eam1105I GACNNNNNGTC 1 cut(s) 394
EciI GGCGGA 1 cut(s) 371
Ecl136II GAGCTC 1 cut(s) 322
Eco130I CCWWGG 1 cut(s) 495
Eco24I GRGCYC 3 cut(s) 219, 324, 473
Eco31I GGTCTC 1 cut(s) 388
Eco47I GGWCC 3 cut(s) 78, 205, 439
Eco53kI GAGCTC 1 cut(s) 322
Eco57I CTGAAG 2 cut(s) 132, 254
Eco88I CYCGRG 1 cut(s) 422
EcoICRI GAGCTC 1 cut(s) 322
EcoO109I RGGNCCY 2 cut(s) 169, 205
EcoT14I CCWWGG 1 cut(s) 495
EcoT38I GRGCYC 3 cut(s) 219, 324, 473
ErhI CCWWGG 1 cut(s) 495
FaiI YATR 1 cut(s) 514
FaqI GGGAC 2 cut(s) 191, 452
FauI CCCGC 2 cut(s) 205, 296
FbaI TGATCA 1 cut(s) 258
FokI GGATG 2 cut(s) 422, 460
FriOI GRGCYC 3 cut(s) 219, 324, 473
GlaI GCGC 2 cut(s) 59, 142
GsuI CTGGAG 1 cut(s) 308
HaeIII GGCC 1 cut(s) 171
HapII CCGG 8 cut(s) 40, 61, 209, 283, 312, 335, 442, 456
HhaI GCGC 2 cut(s) 60, 143
Hin6I GCGC 2 cut(s) 58, 141
HinP1I GCGC 2 cut(s) 58, 141
HincII GTYRAC 1 cut(s) 178
HindII GTYRAC 1 cut(s) 178
HinfI GANTC 2 cut(s) 286, 395
HpaII CCGG 8 cut(s) 40, 61, 209, 283, 312, 335, 442, 456
Hpy166II GTNNAC 1 cut(s) 178
Hpy188I TCNGA 4 cut(s) 234, 291, 319, 388
Hpy188III TCNNGA 1 cut(s) 424
Hpy8I GTNNAC 1 cut(s) 178
Hpy99I CGWCG 3 cut(s) 122, 392, 493
HpyAV CCTTC 2 cut(s) 373, 421
HpyCH4III ACNGT 2 cut(s) 33, 489
HpyCH4V TGCA 4 cut(s) 277, 297, 346, 418
HpyF10VI GCNNNNNNNGC 7 cut(s) 61, 64, 70, 140, 159, 168, 277
HpyF3I CTNAG 1 cut(s) 184
HspAI GCGC 2 cut(s) 58, 141
KroI GCCGGC 2 cut(s) 60, 311
KroNI GCCGGC 2 cut(s) 62, 313
Ksp22I TGATCA 1 cut(s) 258
Kzo9I GATC 2 cut(s) 258, 360
LmnI GCTCC 3 cut(s) 319, 327, 468
Lsp1109I GCAGC 8 cut(s) 57, 76, 130, 140, 143, 146, 289, 430
LweI GCATC 2 cut(s) 17, 405
MaeIII GTNAC 1 cut(s) 401
MalI GATC 2 cut(s) 260, 362
MboI GATC 2 cut(s) 258, 360
MboII GAAGA 3 cut(s) 42, 247, 250
MhlI GDGCHC 3 cut(s) 219, 324, 473
MlyI GAGTC 1 cut(s) 404
MmeI TCCRAC 1 cut(s) 411
MnlI CCTC 6 cut(s) 28, 182, 191, 400, 459, 474
MroNI GCCGGC 2 cut(s) 60, 311
MspA1I CMGCKG 2 cut(s) 70, 253
MspI CCGG 8 cut(s) 40, 61, 209, 283, 312, 335, 442, 456
MspR9I CCNGG 4 cut(s) 209, 283, 335, 457
MvnI CGCG 3 cut(s) 25, 58, 228
MwoI GCNNNNNNNGC 7 cut(s) 61, 64, 70, 140, 159, 168, 277
NaeI GCCGGC 2 cut(s) 62, 313
NciI CCSGG 4 cut(s) 209, 283, 335, 457
NdeII GATC 2 cut(s) 258, 360
NgoMIV GCCGGC 2 cut(s) 60, 311
NlaIV GGNNCC 7 cut(s) 80, 127, 170, 207, 440, 470, 486
NmuCI GTSAC 1 cut(s) 401
PaeR7I CTCGAG 1 cut(s) 422
PdiI GCCGGC 2 cut(s) 62, 313
PfeI GAWTC 1 cut(s) 286
PflMI CCANNNNNTGG 1 cut(s) 337
PinAI ACCGGT 1 cut(s) 441
PleI GAGTC 1 cut(s) 403
PpsI GAGTC 1 cut(s) 403
PpuMI RGGWCCY 1 cut(s) 205
PshAI GACNNNNGTC 1 cut(s) 83
Psp124BI GAGCTC 1 cut(s) 324
Psp5II RGGWCCY 1 cut(s) 205
PspN4I GGNNCC 7 cut(s) 80, 127, 170, 207, 440, 470, 486
PspPI GGNCC 4 cut(s) 78, 169, 205, 439
PspPPI RGGWCCY 1 cut(s) 205
PstNI CAGNNNCTG 2 cut(s) 70, 256
RsaI GTAC 1 cut(s) 511
RsaNI GTAC 1 cut(s) 510
SacI GAGCTC 1 cut(s) 324
Sau3AI GATC 2 cut(s) 258, 360
Sau96I GGNCC 4 cut(s) 78, 169, 205, 439
SchI GAGTC 1 cut(s) 404
ScrFI CCNGG 4 cut(s) 209, 283, 335, 457
SduI GDGCHC 3 cut(s) 219, 324, 473
SetI ASST 8 cut(s) 20, 183, 207, 324, 374, 384, 423, 501
SfaNI GCATC 2 cut(s) 17, 405
Sfr274I CTCGAG 1 cut(s) 422
SinI GGWCC 3 cut(s) 78, 205, 439
SlaI CTCGAG 1 cut(s) 422
SmlI CTYRAG 1 cut(s) 422
SmoI CTYRAG 1 cut(s) 422
SstI GAGCTC 1 cut(s) 324
StyD4I CCNGG 4 cut(s) 207, 281, 333, 455
StyI CCWWGG 1 cut(s) 495
TaaI ACNGT 2 cut(s) 33, 489
TaqI TCGA 2 cut(s) 11, 423
TauI GCSGC 7 cut(s) 28, 58, 70, 126, 225, 228, 256
TfiI GAWTC 1 cut(s) 286
TscAI CASTG 1 cut(s) 353
TseFI GTSAC 1 cut(s) 401
TseI GCWGC 8 cut(s) 64, 70, 143, 153, 156, 159, 277, 418
Tsp45I GTSAC 1 cut(s) 401
TspGWI ACGGA 1 cut(s) 91
TspRI CASTG 1 cut(s) 353
Van91I CCANNNNNTGG 1 cut(s) 337
VpaK11BI GGWCC 3 cut(s) 78, 205, 439
XhoI CTCGAG 1 cut(s) 422
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.