Rorug03G0159400

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0013255
Physical Location & Seq
Forward (+)
13215377 .. 13216000
624 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0159400.1

Sequence Viewer

Length: 624 bp
ATGCACTATGAGAGCGAAGAAGACTACACAACCGTATATAGTGGTGATGAAGATGCAGGGATCTACAGCGCATCCGATGACGATCTTGATCTTCCAGAAGATGACAAAGTCAATGGTTCTGGTGAGATTAATTATACTTTTCTCAAAGAAGAAGAAATTGCCAAGCTTCAGAAAGATTATATTACAGAAGTGTCCACGGTCCTTTCCATATCAAAATCCGATGCATGCTTACTGCTCCTTTACTTTAATTGGAGGGACGAGGAACTAAAGGATGAATGTTTTGCTGATGAAGAGAAAGTTCGAGAGAATGCAGGCTTGTTGGAGAAACCAATGGTTAAACATAGTGCCAGTGCAAATCAACCAATTACTTGCCGGATTTGTTTTGATGAGTACTACTATTCATCAGAGAGTACTATTAGTAGTATTTTGTCAACCTCTTGTGGTCATCCTTATTGTCGAGATTGCTGGGAAGGTTATATTAGCACATTAATCTGTGACTCCGGTCCGGCATGTTTGATGCTGAGATGTCCTGAACCATCTTGTAAGGCAGCAGTTGGTCCTGAATTAATCGAAAACGTGCTGGTGGTGACAGGAAAAAAAGCCGAGTATGACAAGTACAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

207

Amino Acids

23.32

Weight (kDa)

4.33

Isoelectric Point (pI)

55.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UBA_ARI1 PF21235 56 - 89 2.2e-08 E3 ubiquitin-protein ligase ARIH1, UBA-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013255)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G11690 AT5G06380
fragaria_vesca FvH4_6g20920
malus_domestica MD12G1045100.v1.1 MD14G1044000.v1.1
prunus_persica Prupe.7G090300_v2.0.a1
pyrus_communis pycom14g03730
rosa_chinensis RchiOBHm_Chr3g0477261
rosa_laevigata RLG00000023732
rosa_multiflora Rmu_co8078510.1_g000001 Rmu_sc0000348.1_g000009
rosa_roxburghii Rroxscaffold_6G00404280 Rroxscaffold_6G00404760
rosa_rugosa Rorug03G0159400
rosa_samantha Rh3AG209700 Rh3BG243400
rosa_wichuraiana Rw0G000260 Rw0G021730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 68
AcuI CTGAAG 1 cut(s) 152
AfaI GTAC 3 cut(s) 392, 412, 617
AluBI AGCT 1 cut(s) 166
AluI AGCT 1 cut(s) 166
AlwI GGATC 1 cut(s) 68
ApeKI GCWGC 1 cut(s) 548
AseI ATTAAT 3 cut(s) 129, 488, 566
AspLEI GCGC 1 cut(s) 71
AspS9I GGNCC 3 cut(s) 199, 503, 557
AsuHPI GGTGA 3 cut(s) 56, 134, 598
AvaII GGWCC 3 cut(s) 199, 503, 557
BbsI GAAGAC 1 cut(s) 27
BbvI GCAGC 1 cut(s) 560
BccI CCATC 1 cut(s) 544
BfmI CTRYAG 1 cut(s) 64
BisI GCNGC 1 cut(s) 549
BlsI GCNGC 1 cut(s) 550
BmcAI AGTACT 2 cut(s) 392, 412
Bme18I GGWCC 3 cut(s) 199, 503, 557
BmgT120I GGNCC 3 cut(s) 199, 503, 557
BmsI GCATC 4 cut(s) 43, 80, 211, 507
BoxI GACNNNNGTC 1 cut(s) 501
BpiI GAAGAC 1 cut(s) 27
BsaBI GATNNNNATC 2 cut(s) 81, 87
BsaJI CCNNGG 1 cut(s) 195
BsaWI WCCGGW 1 cut(s) 500
Bse1I ACTGG 1 cut(s) 348
Bse8I GATNNNNATC 2 cut(s) 81, 87
BseDI CCNNGG 1 cut(s) 195
BseGI GGATG 3 cut(s) 71, 277, 445
BseJI GATNNNNATC 2 cut(s) 81, 87
BseMII CTCAG 1 cut(s) 512
BseNI ACTGG 1 cut(s) 348
BseXI GCAGC 1 cut(s) 560
BseYI CCCAGC 1 cut(s) 465
BsiSI CCGG 3 cut(s) 373, 501, 506
BslFI GGGAC 1 cut(s) 269
BsmFI GGGAC 1 cut(s) 269
BsmI GAATGC 1 cut(s) 313
Bsp143I GATC 3 cut(s) 60, 82, 88
BspCNI CTCAG 1 cut(s) 513
BspPI GGATC 1 cut(s) 68
BsrI ACTGG 1 cut(s) 348
BssECI CCNNGG 1 cut(s) 195
BssMI GATC 3 cut(s) 60, 82, 88
Bst4CI ACNGT 2 cut(s) 34, 199
Bst6I CTCTTC 1 cut(s) 285
BstC8I GCNNGC 2 cut(s) 226, 313
BstDEI CTNAG 1 cut(s) 521
BstDSI CCRYGG 1 cut(s) 195
BstF5I GGATG 3 cut(s) 71, 277, 445
BstHHI GCGC 1 cut(s) 71
BstKTI GATC 3 cut(s) 63, 85, 91
BstMBI GATC 3 cut(s) 60, 82, 88
BstNSI RCATGY 2 cut(s) 228, 513
BstPAI GACNNNNGTC 1 cut(s) 501
BstSFI CTRYAG 1 cut(s) 64
BstV1I GCAGC 1 cut(s) 560
BstV2I GAAGAC 1 cut(s) 27
BstX2I RGATCY 1 cut(s) 60
BstYI RGATCY 1 cut(s) 60
BtgI CCRYGG 1 cut(s) 195
BtsCI GGATG 3 cut(s) 71, 277, 445
BtsIMutI CAGTG 1 cut(s) 355
Cac8I GCNNGC 2 cut(s) 226, 313
CfoI GCGC 1 cut(s) 71
Cfr13I GGNCC 3 cut(s) 199, 503, 557
CpoI CGGWCCG 1 cut(s) 503
Csp6I GTAC 3 cut(s) 391, 411, 616
CspI CGGWCCG 1 cut(s) 503
CviAII CATG 2 cut(s) 225, 510
CviJI RGCY 3 cut(s) 166, 315, 602
CviKI_1 RGCY 3 cut(s) 166, 315, 602
CviQI GTAC 3 cut(s) 391, 411, 616
DdeI CTNAG 1 cut(s) 521
DpnI GATC 3 cut(s) 62, 84, 90
DpnII GATC 3 cut(s) 60, 82, 88
Eam1104I CTCTTC 1 cut(s) 285
EarI CTCTTC 1 cut(s) 285
Eco47I GGWCC 3 cut(s) 199, 503, 557
Eco57I CTGAAG 1 cut(s) 152
EcoT22I ATGCAT 1 cut(s) 226
FaeI CATG 2 cut(s) 228, 513
FaqI GGGAC 1 cut(s) 269
FatI CATG 2 cut(s) 224, 509
Fnu4HI GCNGC 1 cut(s) 549
FokI GGATG 3 cut(s) 58, 284, 432
Fsp4HI GCNGC 1 cut(s) 549
GlaI GCGC 1 cut(s) 70
GluI GCNGC 1 cut(s) 549
GsaI CCCAGC 1 cut(s) 469
HapII CCGG 3 cut(s) 373, 501, 506
HhaI GCGC 1 cut(s) 71
Hin1II CATG 2 cut(s) 228, 513
Hin6I GCGC 1 cut(s) 69
HinP1I GCGC 1 cut(s) 69
HincII GTYRAC 1 cut(s) 432
HindII GTYRAC 1 cut(s) 432
HindIII AAGCTT 1 cut(s) 164
HinfI GANTC 1 cut(s) 497
HpaII CCGG 3 cut(s) 373, 501, 506
HphI GGTGA 3 cut(s) 56, 134, 598
Hpy166II GTNNAC 2 cut(s) 195, 432
Hpy188I TCNGA 4 cut(s) 76, 171, 220, 406
Hpy188III TCNNGA 6 cut(s) 86, 95, 302, 458, 530, 560
Hpy8I GTNNAC 2 cut(s) 195, 432
HpyAV CCTTC 1 cut(s) 464
HpyCH4III ACNGT 2 cut(s) 34, 199
HpyCH4IV ACGT 1 cut(s) 576
HpyCH4V TGCA 5 cut(s) 4, 56, 224, 311, 353
HpyF3I CTNAG 1 cut(s) 521
HpySE526I ACGT 1 cut(s) 576
Hsp92II CATG 2 cut(s) 228, 513
HspAI GCGC 1 cut(s) 69
Kzo9I GATC 3 cut(s) 60, 82, 88
LmnI GCTCC 1 cut(s) 240
Lsp1109I GCAGC 1 cut(s) 560
LweI GCATC 4 cut(s) 43, 80, 211, 507
MaeII ACGT 1 cut(s) 576
MaeIII GTNAC 2 cut(s) 494, 586
MalI GATC 3 cut(s) 62, 84, 90
MboI GATC 3 cut(s) 60, 82, 88
MboII GAAGA 8 cut(s) 29, 32, 62, 83, 110, 161, 164, 302
MflI RGATCY 1 cut(s) 60
MluCI AATT 5 cut(s) 130, 156, 247, 363, 563
MlyI GAGTC 1 cut(s) 491
MmeI TCCRAC 1 cut(s) 300
MnlI CCTC 3 cut(s) 246, 253, 445
Mph1103I ATGCAT 1 cut(s) 226
MseI TTAA 5 cut(s) 129, 246, 336, 488, 566
MspI CCGG 3 cut(s) 373, 501, 506
Mva1269I GAATGC 1 cut(s) 313
NdeII GATC 3 cut(s) 60, 82, 88
NlaIII CATG 2 cut(s) 228, 513
NmuCI GTSAC 2 cut(s) 494, 586
NsiI ATGCAT 1 cut(s) 226
NspI RCATGY 2 cut(s) 228, 513
PaeI GCATGC 1 cut(s) 228
PctI GAATGC 1 cut(s) 313
PflFI GACNNNGTC 1 cut(s) 107
PkrI GCNGC 1 cut(s) 550
PleI GAGTC 1 cut(s) 491
PpsI GAGTC 1 cut(s) 491
PshAI GACNNNNGTC 1 cut(s) 501
PshBI ATTAAT 3 cut(s) 129, 488, 566
PspFI CCCAGC 1 cut(s) 465
PspPI GGNCC 3 cut(s) 199, 503, 557
PsuI RGATCY 1 cut(s) 60
PsyI GACNNNGTC 1 cut(s) 107
RsaI GTAC 3 cut(s) 392, 412, 617
RsaNI GTAC 3 cut(s) 391, 411, 616
Rsr2I CGGWCCG 1 cut(s) 503
RsrII CGGWCCG 1 cut(s) 503
SaqAI TTAA 5 cut(s) 129, 246, 336, 488, 566
SatI GCNGC 1 cut(s) 549
Sau3AI GATC 3 cut(s) 60, 82, 88
Sau96I GGNCC 3 cut(s) 199, 503, 557
ScaI AGTACT 2 cut(s) 392, 412
SchI GAGTC 1 cut(s) 491
SetI ASST 4 cut(s) 168, 437, 475, 579
SfaNI GCATC 4 cut(s) 43, 80, 211, 507
SfcI CTRYAG 1 cut(s) 64
SinI GGWCC 3 cut(s) 199, 503, 557
SphI GCATGC 1 cut(s) 228
Sse9I AATT 5 cut(s) 130, 156, 247, 363, 563
TaaI ACNGT 2 cut(s) 34, 199
TaiI ACGT 1 cut(s) 579
TaqI TCGA 3 cut(s) 301, 457, 570
TasI AATT 5 cut(s) 130, 156, 247, 363, 563
TatI WGTACW 3 cut(s) 390, 410, 615
Tru1I TTAA 5 cut(s) 129, 246, 336, 488, 566
Tru9I TTAA 5 cut(s) 129, 246, 336, 488, 566
TscAI CASTG 1 cut(s) 355
TseFI GTSAC 2 cut(s) 494, 586
TseI GCWGC 1 cut(s) 548
Tsp45I GTSAC 2 cut(s) 494, 586
TspDTI ATGAA 4 cut(s) 63, 288, 303, 390
TspRI CASTG 1 cut(s) 355
Tth111I GACNNNGTC 1 cut(s) 107
VpaK11BI GGWCC 3 cut(s) 199, 503, 557
VspI ATTAAT 3 cut(s) 129, 488, 566
XceI RCATGY 2 cut(s) 228, 513
ZrmI AGTACT 2 cut(s) 392, 412
Zsp2I ATGCAT 1 cut(s) 226
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.